Research in the Klamath Basin 20 07 Annual Report Russet Potato Variety Development, 2007 Brian A. Charlton and Darrin A. Culp1 Introduction Since its inception in 1985, the Tri-State variety development program has primarily focused on the development of processing and dual-purpose (processing and fresh market) russets. During the past decade, Oregon has been the lead state in the release of nine varieties and is in the process of releasing two additional russets in 2008. In total, more than 25 new varieties have been released by the Tri-State variety development program since 1985. Although the development of russet varieties remain the primary focus, recent efforts have included red-skinned and specialty-type selections. Many of these selections offer unique skin and/or flesh color combinations along with enhanced nutritional qualities including elevated antioxidant and Vitamin C content. Breeding efforts have focused more on improving genetic resistance to various pests and diseases as a means of lowering production costs. For example, screening for resistance to foliar and tuber late blight occurs in Corvallis, Oregon as environmental conditions are conducive to disease development. High insect and disease pressure occurs in the long growing-season of the Columbia Basin which makes the Hermiston Agricultural Research & Extension Center (HAREC) an ideal screening location for potato virus Y (PVY), potato leaf roll virus (PLRV) and associated problems of net necrosis, early die syndrome, and more recently potato tuber moth (Phthorimaea operculella (Zeller)). Screening for resistance to various species of nematodes and related diseases is being accomplished at several locations. The Klamath Basin Research and Extension Center (KBREC) routinely screen selections for resistance to root-knot nematode (Meloidogyne chitwoodi and Meloidogyne hapla) and corky ringspot disease resulting from infection of Tobacco rattle virus which is vectored by stubby-root (Paratrichodorus spp.) nematodes. Other cooperating sites within the Tri-State area also work on resistant screening and other production limitations most suited to their respective location. The overall objective is that future releases will offer genetic resistance to many economically important pests and diseases which will help reduce production inputs as these continue to rise in cost. KBREC participation in the Tri-State evaluation of new russet lines includes the following: Preliminary Yield Trial 2 (PYT-2), Statewide Russet Trial, and the late-season Western Regional Russet Trial. In general, selections currently in the PYT-2 trial were selected two to three years earlier from single-hill selections. Clones advancing from the PYT-2 trial enter the Statewide Russet trial the following year and may advance after two 1 Assistant Professor and Faculty Research Assistant, respectively, Klamath Basin Research and Extension Center, Klamath Falls, OR. Acknowledgements: Partial financial support for this program from the Oregon Potato Commission; the Cooperative State Research, Extension, and Education Service (CSREES); and the USDA Agricultural Research Service (ARS) is gratefully recognized. Reference to a product or company is for specific information only and does not endorse or recommend that product or company to the exclusion of others that may be suitable. ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 1 Research in the Klamath Basin 20 07 Annual Report years of testing to the Tri-State trial. Clones graduating from the Tri-State trial advance to the Western Regional Russet Trial which also includes material from breeding programs in Colorado and Texas. Oregon selections remain in the Oregon Statewide Trial until they complete regional evaluation or are discarded. In most circumstances, a period of 12 to 15 years is required to release a variety following the actual breeding cross. Procedures Trials were conducted on a Poe fine sandy loam soil at KBREC. The soil has a pH of 6.8 and an organic matter content of 1.02 percent in the plow layer. The location was cropped to teff grass hay the previous year, which was treated with roundup during the fall of 2006 to aid in breakdown of material through the winter. Gypsum was applied at a rate of 2000 lbs/acre on April 11. Field preparation included discing twice, rototilling once, and moldboard plowing once to break up sod in mid-April 2007. Fumigation treatments included TeloneTM II (dichloropropene, Dow AgroSciences) at 15 gal/acre (gpa) and Vapam (sodium methyldithiocarbamate, AMVAC) at 25 gpa on April 26, 2007, followed by several harrowing passes on May 7. Dry pre-plant fertilizer was broadcasted on May 9 followed by liquid fertilizer applied at bed formation on May 10. Total pre-plant fertilizer included 165 lb Nitrogen (N), 80 lb Phosphate (P2O5), 100 lb Potash (K20) and 140 lb Sulfur (S). Seed for all trials was hand cut to approximately 1.5-2.0 oz/seed piece on May 9, 10 and 11, treated with Tops MZ (thiophamate-methyl, mancozeb, Bayer CropScience), and suberized for 2 weeks at 550F and approximately 95 percent relative humidity. All trials (Preliminary (PYT-2) Russet, Statewide Russet, and Regional Russet) were planted at 9-inch spacing in 36-inch rows with an assisted-feed, 2-row planter on May 23. The insecticide Admire Pro (imidacloprid, Bayer CropScience) at 5-6 fl. oz. / acre and the fungicide Quadris (azoxystrobin, Syngenta) at 6-8 fluid oz. / acre were applied in-furrow over the seed piece. Planted rows were bedded up and leveled off with a rolling cultivator and drag bar followed by a pre-emergence application of Dual II Magnum (s-metalachlor, Syngenta) at 1.3 pt./ acre and Prowl H2O (pendimethalin, BASF) at 1.5 pt./ acre on June 12 for broadleaf and grass weed control. Immediately following application, 0.43 inches of irrigation was supplied to incorporate the herbicides. Matrix (rimsulfuron, Dupont) was applied post-emergence at 1 oz. / acre on July 5 and incorporated with 0.25 inches of irrigation. A total of 19.7 inches of water (irrigation plus rainfall) was applied during the growing season with sprinklers arranged in a 40- by 48-ft. pattern. All trials were arranged in a randomized complete block design. The PYT-2 Russet Trial included 2 replications of 20-hill plots. Entries for the PYT-2 Russet Trial included standard varieties Russet Burbank, Ranger Russet, Shepody, and Russet Norkotah, and 66 numbered selections. The Statewide Russet Trial had 4 replications of 30-hill plots and included 15 numbered selections in addition to the same standard varieties as those in the PYT-2 trial. The Western Regional Russet Trial had 4 replications of 30-hill plots. Ranger Russet, Russet Burbank, and Russet Norkotah were the standard varieties along with 16 numbered selections. ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 2 Research in the Klamath Basin 20 07 Annual Report Plant stands were monitored on June13 and 27. Vine vigor was noted on July 25. On July 26 the fungicide Ridomil Gold Bravo (mefenoxam/chlorothalinil, Syngenta) and the insecticide Leverage (imidacloprid, cyfluthrin, Bayer CropScience) was applied aerially. Another application of Ridomil Gold was applied on August 10 at labeled rates. Plant maturity assessments were done September 6 followed by vine rolling and Reglone (diquat dibromide, Syngenta) application at 1.5 pt. /acre with a ground sprayer on September 10. Tubers were harvested using a one-row digger-bagger on October 5 (Regional Russet), October 8 (PYT-2 Russet), and October 9 (Statewide Russet). All tubers from each plot were stored at 550F until grading in early to mid November. Using USDA potato grade standards, each plot was graded into U.S. No. 1s, No. 2s, B’s (under 4 oz.) and culls. U.S. No. 1s were separated by weight to 4- to 8-oz, 8- to 12-oz, and over 12-oz groupings. Sub-samples of approximately 10 lbs from the 8- to 12 oz. fraction were used to determine specific gravity by the weight-in-air, weight-in-water method. Ten tubers from the over 12-oz were selected at random and cut lengthwise to determine internal defects. External characteristics were noted for each replication. Potatoes were downgraded to No. 2s or culls if growth cracks, knobs, or misshapen tubers were severe enough according to USDA grade standards. Yields for U.S. No. 1s were not adjusted to account for external blemishes such as Rhizoctonia or silver scurf or internal defects such as hollow heart, brown center, and others. With exception of the PYT-2 Russet Trial, data collected was analyzed using SAS for Linear Models 9.1 (SAS Institute Inc.) software. Least significant differences (LSDs) were calculated based on the student’s t test at the 5 percent probability level. Only a portion of the data obtained is reported here. Data from all trial locations were summarized and reports were reviewed by all cooperators on January 3 and 4, 2008 as the basis for decisions on disposition of trial entries. Results and Discussion Preliminary Yield Trial A partial summary of yield characteristics of the clones selected for further evaluation based on performance at KBREC is presented in Table 1. Eleven numbered selections out of 66 were retained for further evaluation. Ideally, clones retained must perform well at all locations; however, vast differences in climate, insect and disease pressures, and other production scenarios among the testing locations prevent such performance uniformity. Therefore, several selections are retained because of superior performance at one location. In general, total yield of retained selections were slightly lower than those of the standard varieties; however, yields of U.S. No. 1’s were generally higher. Specific gravities were exceptionally high in 2007 compared to previous years with several selections exceeding the 1.088 cap at which processing contracts begin to assess penalties. In general, the ‘OR’ clones and POR05V016-2 were retained for genetic resistances to various pests and diseases. These selections had poorer tuber appearance than the standard entries and would likely find difficulty in meeting fresh market appearance standards with the exception of POR05V016-2 which possessed very ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 3 Research in the Klamath Basin 20 07 Annual Report uniform tuber appearance. AO01114-4, AO02060-3, and AO03003-3 performed exceptionally well at KBREC and had excellent tuber appearance. Statewide Yield Trial All entries in this trial achieved excellent stands (Table 2) Most of the numbered selections were similar in maturity to Russet Burbank and Ranger Russet with all selections being later in maturity compared to Russet Norkotah. Hollow heart was not a problem in 2007 compared to previous years; however, Russet Norkotah and AO96141-3 exhibited 15 and 13 percent, respectively. Although, excessive tuber size in these entries undoubtedly skewed these values to some degree. Corky ringspot was problematic in Ranger Russet, AO96164-1, AO96305-3, and AO98286-4. Three of the top yielding selections (AO96365-2, AO98282-5, and AO01057-5) at KBREC were retained for further evaluation (Table 3). Of these, AO98282-5 had the most attractive tuber appearance for fresh market. AO96365-2 tended to be quite round and most suitable for processing into chips. AO96164-1 and AO96141-3 have both performed exceptionally well in the Western Regional trial. Both have produced very consistent processing quality across all Tri-state (Oregon, Washington, and Idaho) locations. Oregon plans to lead the release of AO96164-1 in 2008. As seen in the Preliminary Yield trial, specific gravities were generally very high with the majority of entries exceeding the 1.088 threshold at which processing contracts begin to assess penalties. Total yields of retained selections were generally comparable to the four standard entries. U.S. No. 1 yields were generally higher than those of Russet Burbank and lower than Russet Norkotah with the exception of AO96365-2 and AO01057-5 (Table 3). Western Regional Trial Excellent stands were achieved by all entries in the trial. All entries were later in maturity than Russet Norkotah (Table 4). A relatively high incidence of hollow heart was observed for A96104-2, AOTX95265-2ARu and TXA549-1Ru. Vascular discoloration was problematic for A97287-6 and corky ringspot was observed in varying degrees in nearly every entry. Russet Norkotah has rarely exhibited corky ringspot (CRS) symptoms in the Klamath Basin which suggests a possible difference in virus strains compared to the Columbia Basin where Russet Norkotah routinely shows CRS symptoms. Other entries exhibiting zero or low CRS symptoms were AO96164-1, AOTX95265-3Ru, CO97087-2Ru, and CO97138-7Ru. Standard varieties produced total yields similar to those observed in the Statewide Trial (Table 5). Seven selections have been included in the trial for 3 years and have graduated. The disposition of these selections will be determined by the sponsoring states. A95409-1, A96104-2, AOA95154-1, and AOA95155-7 are Idaho selections, AO96164-1 is an Oregon selection, CO95172-3Ru is from Colorado, and TXA549-1Ru is a Texas selection. Release decisions have not been made for any of these selections with the exception of AO96164-1 which is planned for release in 2008. Three selections dropped for various reasons included A97287-6, CO97138-3Ru, and CO97138-7Ru. Selections producing acceptable yields and attractive tuber appearance comparable to Russet Norkotah at KBREC include A96104-2 and AOTX95265-4Ru. Both of these selections will be evaluated in cultural management trials at KBREC in 2008. ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 4 Research in the Klamath Basin 20 07 Annual Report Table 1. Tuber yield and specific gravity of potato entries selected from the Preliminary Yield Trial grown at the Klamath Basin Research and Extension Center, Klamath Falls, OR, 2007. Variety or selection Yield U.S. No. 1s 4-8 oz 8-12 oz >12 oz Yield Specific total <4 oz No. 2s culls total gravity cwt/acre Russet Burbank 120 116 75 311 52 162 136 661 1.093 Ranger Russet 90 177 133 399 64 90 35 588 1.099 Shepody 59 108 158 325 25 185 123 658 1.077 Russet Norkotah 122 156 130 409 48 64 49 570 1.082 AO01114-4 53 124 238 416 26 38 27 507 1.099 AO02060-3 45 167 283 495 28 73 33 628 1.097 AO02118-2 81 120 225 426 28 64 20 538 1.084 AO03003-3 121 255 167 543 102 49 39 733 1.082 AO03096-5 156 176 54 385 61 44 7 497 1.113 OR03085-5 181 194 124 499 20 32 6 557 1.097 OR03151-4 135 174 196 505 57 80 60 702 1.089 OR04018-5 49 172 172 393 44 80 57 574 1.094 OR04057-2 102 165 83 350 137 91 24 602 1.095 OR04062-1 142 165 51 357 63 24 13 457 1.100 POR05V016-2 149 222 147 518 54 52 5 629 1.085 Mean1 107 166 149 422 54 75 42 593 1.092 1 Mean for standard varieties and selected clones only. ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 5 Research in the Klamath Basin 20 07 Annual Report Table 2. Characteristics of potato entries in the Oregon Statewide Trial grown at the Klamath Basin Research and Extension Center, Klamath Falls, OR, 2007. Variety or selection Percent Vine stand Tuber characteristics3 Vine 1 2 vigor maturity skin color Hollow russeting eyes shape heart 4 Internal Corky 4 brown spot ring spot4 % Russet Burbank Ranger Shepody Norkotah 99 99 100 100 4.8 5.0 4.0 3.5 2.4 3.4 2.5 1.6 4.1 4.0 1.0 4.3 4.4 4.3 1.8 4.5 4.0 3.0 4.0 3.9 4.1 5.0 3.9 4.8 0 0 0 15 25 33 18 0 0 25 0 0 AO96164-1# AO96141-3* AO96305-3* AO96365-2* AO98282-5* 98 98 100 100 99 5.0 5.0 4.0 4.3 5.0 2.1 3.6 2.5 3.5 2.8 4.0 4.0 3.8 4.0 4.0 4.3 4.8 4.0 5.0 5.0 4.1 3.8 4.1 3.5 3.5 4.1 4.9 4.1 3.0 4.0 0 13 0 0 3 13 0 20 3 0 13 0 13 8 0 AO00057-2* AO98259-6 AO98286-4 AO01057-5* AO02027-6 99 98 95 99 99 4.3 3.5 3.3 4.0 5.0 3.1 2.8 2.6 2.3 1.9 4.0 4.0 3.0 4.1 4.0 4.5 4.5 3.0 4.8 4.3 4.1 3.8 4.3 3.8 3.8 3.8 3.8 3.5 3.6 3.9 0 0 0 0 0 13 15 28 3 8 5 0 25 0 3 AO02103-1 AO02182-1* AO02183-2* AO02196-5 OR03029-2 98 98 98 99 99 3.8 5.0 5.0 5.0 3.3 2.6 1.9 3.4 3.8 2.9 4.4 4.1 4.0 4.1 4.1 5.0 4.1 4.8 4.5 5.0 3.4 4.1 3.9 4.1 4.3 3.8 4.5 5.0 4.1 2.8 0 0 5 0 0 25 5 20 10 0 8 8 0 3 0 98.8 ------- 4.3 ------- 2.7 ------- 3.8 ------- 4.3 ------- 3.9 ------- 4.0 ------- 1.8 194.4 5.1 12.4 122.8 21.5 5.7 224.1 NS Mean CV(%) LSD (0.05) 1/ Vine vigor: 1 = small, weak; 5 = large, robust. 2/ Vine maturity: 1 = early; 5 = late. 3/ Skin color: 1 = light; 5 = dark. Russeting: 1 = light; 5 = heavy. Eyes: 1 = deep; 5 = shallow. Shape: 1 = round, 5 = long. 4/ Percent in 10 large tuber sample *Retained for further evaluation # To be released ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 6 Research in the Klamath Basin 20 07 Annual Report Table 3. Tuber yield by grade and specific gravity in the Oregon Statewide Trial grown at the Klamath Basin Research and Extension Center, Klamath Falls, OR, 2007. Variety or selection 4-8 oz Yield U.S. No. 1s 8-12 oz >12 oz Yield total Specific Bs No. 2s culls total 1 gravity rank cwt/acre Russet Burbank Ranger Shepody Norkotah 159 86 66 136 101 139 98 150 22 126 106 116 281 350 270 402 86 74 37 51 84 60 115 29 61 20 45 15 512 504 468 497 1.093 1.096 1.085 1.078 13 7 14 3 AO96164-1# AO96141-3* AO96305-3* AO96365-2* AO98282-5* 154 82 91 239 157 101 127 124 151 132 42 145 34 90 96 296 355 248 479 385 89 73 156 65 67 29 98 4 37 34 10 39 8 5 31 425 565 416 587 518 1.087 1.101 1.093 1.092 1.100 11 6 15 1 4 AO00057-2* AO98259-6 AO98286-4 AO01057-5* AO02027-6 110 110 169 146 100 132 135 136 158 101 94 40 57 127 17 336 284 363 430 218 43 34 54 43 61 37 34 31 24 117 14 19 26 8 54 430 371 474 505 450 1.089 1.084 1.077 1.088 1.091 8 12 5 2 18 AO02103-1 AO02182-1* AO02183-2* AO02196-5 OR03029-2 106 96 48 97 137 122 128 113 96 26 95 103 62 44 4 323 326 223 236 167 30 47 151 68 195 60 24 35 76 18 25 13 34 46 9 438 410 443 426 390 1.086 1.078 1.100 1.101 1.106 10 9 17 16 19 120.3 24.8 42.2 119.4 24.3 41.1 74.7 47.2 49.9 314.4 22.4 99.6 75.1 26.3 28.0 49.7 41.3 29.1 25.4 61.6 22.2 464.6 16.0 105.4 1.091 0.3 0.005 Mean CV (%) LSD (0.05) 1/ Rank: ranking in total U.S. No. 1 yield for KBREC out of 19 entries. *Retained for further evaluation # To be released ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 7 Research in the Klamath Basin 20 07 Annual Report Table 4. Characteristics of potato entries in the Western Regional Russet Trial grown at the Klamath Basin Research and Extension Center, Klamath Falls, OR, 2007. Variety or Percent Vine Tuber characteristics3 Vine 1 2 vigor maturity Hallow skin color russeting eyes shape heart 4 Vascular Corky 4 discoloration ring spot4 selection stand Ranger Russet Russet Burbank Russet Norkotah % 98 100 99 4.3 5.0 4.0 3.9 2.9 1.8 3.9 4.0 4.3 4.4 4.8 5.0 3.1 3.9 3.9 5.0 4.4 4.5 0 0 5 % 0 0 0 58 23 0 A95409-1 A96104-2 A97287-6 AC96052-1RU AO96141-3 100 100 98 99 100 4.0 4.8 5.0 5.0 5.0 3.8 2.8 3.6 3.3 3.5 3.4 4.0 3.4 4.3 3.8 3.9 4.9 4.0 5.0 4.9 3.5 3.5 3.1 4.0 3.8 3.8 4.3 4.0 3.6 4.9 0 33 0 5 0 5 3 20 3 3 28 15 13 28 20 AO96164-1 AOA95154-1 AOA95155-7 AOTX95265-2ARu AOTX95265-3Ru 100 99 100 96 99 5.0 4.8 4.5 4.3 4.5 2.5 3.4 3.5 2.0 2.5 4.0 4.0 3.6 4.5 4.4 4.5 4.8 4.8 5.0 5.0 4.0 4.1 4.1 3.9 3.8 4.6 3.9 3.8 4.0 4.5 0 3 0 10 5 0 0 0 5 3 3 18 15 8 3 AOTX95265-4Ru CO95172-3RU CO97087-2RU CO97138-3RU CO97138-7RU TXA549-1Ru 98 99 99 99 98 99 4.5 5.0 4.3 4.0 3.3 4.3 2.1 3.5 2.6 2.0 3.4 3.8 4.1 4.0 3.9 4.0 3.8 4.0 5.0 5.0 4.9 5.0 4.0 4.4 3.8 3.8 4.0 4.0 3.6 4.0 4.4 4.0 4.4 4.3 4.6 3.0 8 3 0 0 3 13 0 0 0 0 3 0 5 18 0 5 0 5 Mean CV (%) LSD (0.05) 99 ------- 4.5 3.0 ------- 4.0 ------- 4.7 ------- 3.8 ------- 4.2 ------- 4.5 134.5 8.5 2.2 240.3 7.6 13.7 147.9 28.7 1/ Vine vigor: 1 = small, weak; 5 = large, robust. Vine maturity: 1 = early; 5 = late. 3/ Skin color: 1 = light; 5 = dark. Russeting: 1 = light; 5 = heavy. Eyes: 1 = deep; 5 = shallow. Shape: 1 = round, 5 = long. 4/ Percent in 10 large tuber sample 2/ ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 8 Research in the Klamath Basin 20 07 Annual Report Table 5. Tuber yield by grade and specific gravity in the Western Regional Russet Trial grown at the Klamath Basin Research and Extension Center, Klamath Falls, OR, 2007. Variety or selection Yield U.S. No. 1s 4-8 oz 8-12 oz >12 oz Yield total <4 oz Specific No. 2s culls total 1 gravity rank cwt/acre Ranger Russet Russet Burbank Russet Norkotah 89 119 126 146 113 152 90 65 144 325 297 422 70 76 53 74 109 59 26 46 16 495 528 549 1.097 1.091 1.075 13 14 3 A95409-1 A96104-2 A97287-6 AC96052-1RU AO96141-3 99 100 115 101 52 130 156 139 90 97 174 112 100 49 63 403 368 354 239 211 41 58 48 46 96 68 36 84 15 53 11 10 24 15 31 522 472 510 315 391 1.095 1.086 1.090 1.088 1.101 4 6 8 18 19 AO96164-1 171 AOA95154-1 126 AOA95155-7 125 AOTX95265-2ARu 94 AOTX95265-3Ru 98 117 122 94 132 130 94 37 34 141 110 382 285 254 368 338 82 75 70 41 50 120 13 44 44 62 15 3 5 27 39 599 376 372 479 489 1.090 1.091 1.083 1.076 1.078 5 15 16 7 12 AOTX95265-4Ru CO95172-3RU CO97087-2RU CO97138-3RU CO97138-7RU TXA549-1Ru 82 160 124 83 90 88 127 134 83 145 135 130 134 52 37 117 229 209 344 346 244 346 454 427 39 85 60 30 31 30 57 49 92 31 78 79 22 17 16 10 25 42 461 497 412 417 589 578 1.077 1.095 1.090 1.079 1.077 1.088 11 9 17 10 1 2 Mean CV (%) LSD (0.05) 107 25 39 125 25 44 105 45 67 337 20 96 57 26 21 61 51 44 21 82 25 476 14 94 1.087 0.362 0.006 ---------- 1/ Rank: ranking in total U.S. No. 1 yield for KBREC out of 19 entries. ______________________________________________________________________________ Klamath Basin Research and Extension Center Russet Potato Variety Development, 2007 Page 9