CAT Nº: 2054
Peptone 10.00 Sodium Thiosulphate 10.00
Special Bilis 5.00 Sodium Citrate
Yeast extract
Sucrose
Glucose
3.00
1.00
Sodium Cholate
20.00 Sodium Chloride
Chromogenic Mix
3.00
10.00
2.49
Lactose 0.10 Bacteriological Agar 15.00
Suspend 90 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution AVOID OVERHEATING. DO NOT AUTOCLAVE. Dispense into appropriate containers. The color of the prepared medium is amber. The prepared medium should be stored at 8-15ºC.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes, discard the medium.
VIBRIO CHROMOGENIC AGAR is recommended for isolation and selective differentiation of Vibrio species based on color development depending on their β-galactosidase and β-glucosidase enzyme activities.
The medium contains Yeast extract and Peptones which are the source of nitrogen, vitamins (particularly the B-group essential for bacterial growth), minerals and amino acids. Special Bilis are inhibitors of Gram-positive organisms.
Sucrose, glucose and Lactose ate the fermentable carbohydrates providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Sodium citrate, Sodium thiosulfate and Sodium cholate are the selective agents, inhibiting the Gram positive bacteria. Chromogenic substrate is added to detect Vibrio species by means of a color change. The released chromophore in Vibrio Chromogenic Agar is colored and target colonies are easily identified. It is designed for the development and diferentation of 3 types of Vibrio depending on enzyme of each activity. β-glucosidase activity will appear as blue-green colonies representing
; β galactosidade activity will show pink – red colonies representing
. Finally yellowish-white colonies will represent
which has β-galactosidase but does not use it due to the high concentration of sugars. The alkaline pH of the medium enhances the recovery of
.
.
The genus Vibrio consists of micro-organisms whose natural habitat is marine and fluvial ecosystems. They are frequently isolated from marine water, especially in warmer months and when the water temperature is higher than
17ºC. Vibrio species are mainly responsible for causing cholera and food poisoning in humans.
causes secretory diarrhea (cholera) due to the intake of contaminated food such as raw oysters.
is a
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major cause of food borne infections, causing food poisoning. In addition, other species as for example, V. alginolyticus, extra intestinal infections such as otitis, and infections of injury, septicemia and meningitis.
ISO 21872-1:2007 recommend a preenrichment of Vibrio species in Alkaline Peptone Water (Cat. 1407) in order to increase the recovery. Inoculate the medium and incubate at 35ºC±2ºC for 24-48 hours.
The following results were obtained from standard strains, after incubation at a temperature of 35ºC±2ºC and observed after 24-48 hours.
Microorganisms Growth Colony colour
Vibrio cholerae ATCC 14034
Vibrio alginolyticus ATCC 17749
Vibrio parahaemolyticus ATCC 17802
Psedomonas aeruginosa ATCC 27853
Good
Good
Good
Inhibited
Pink-Rose
Colorless
Green-Blue
Isabel Mª García Bermejo. Diagnóstico de las infecciones humanas causadas por especies halófilas del género Vibrio. Servicio de
Microbiología, Hospital de Getafe Koneman. Diagnostico Microbiológico/ Microbiological diagnosis: Texto Y Atlas En Color. Stephen Allen
Ed. Médica Panamericana, 30/06/2008 Romero Cabello, Raúl.
Rodríguez, E; Gamboa, M; Hernández, F; García, J. 2005. Microbiología y Parasitología Humana. Bases etiológicas de las enfermedades infecciosas y parasitarias. 3ª Edición. Médica Panamericana.
H.Y. Kudo et. al, Improved Method for Detection of Vibrio parahaemolyticus in Seafood. ASM. Vol 67, No. 12, pg 5819-5823 (2001)
PTechnical Specification ISO/TS 21872-1:2007(E) Microbiology of food and animal feeding stuffs-Horizontal method for the detection of potentially enteropathogenic Vibrio spp. Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae. Technical Specification ISO/TS
21872-2:2007(E) Microbiology of food and animal feeding stuffs-Horizontal method for the detection of potentially enteropathogenic
Vibrio spp. Part 2: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae.
8ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
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