TRYPTICASEIN SOY BLOOD AGAR BASE
CAT Nº: 1189
For the cultivation of fastidious microorganisms and detection
Of hemolytic activity of fastidious microorganisms
FORMULA IN g/l
Pancreatic Digest of Casein
14.50
Growth Factors
1.50
Soy Peptone
5.00
Bacteriological Agar
14.00
Sodium Chloride
5.00
Final pH 7.3 ± 0.2 at 25ºC
PREPARATION
Suspend 40 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation.
Boil for one minute until complete dissolution. Sterilize in autoclave at 121°C for 15 minutes. Cool to 45 - 50°C and add
50 ml of sterile defibrinated blood. Homogenize gently and pour into Petri dishes. Be careful to avoid bubble formation
when adding the blood. The prepared medium should be stored at 2-8°C. The color without blood is amber, slightly
opalescent. The prepared medium with blood is red.
The dehydrated medium should be homogeneous, free-flowing and beige in color. If there are any physical changes,
discard the medium.
USES
BLOOD AGAR BASE is used for the isolation, cultivation and detection of hemolytic reaction of fastidious microorganisms.
It is suitable for isolating and cultivating a wide range of microorganisms with difficult growth characteristics. Upon
adding blood, it can be utilized for determining hemolytic reactions.
Pancreatic digest of casein and Soy peptone provide nitrogen, vitamins, minerals and amino acids essential for growth.
Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying
agent.
The addition of blood provides extra growth factors for fastidious microorganisms and is the basis for determining
hemolytic reactions. Hemolytic patterns may vary with the type of blood or base medium used. For example,
defibrinated sheep blood allows the recovery of Thermophilus species and gives best results for Group A streptococci.
Inoculate and incubate at 37 ± 2°C for 24 hours.
Results:
1.Alpha-hemolysis: greenish discoloration of medium
2.Beta-hemolysis: clear zone surrounding colony
3.Gamma-hemolysis: no change
MICROBIOLOGICAL TEST
The following results were obtained in the performance of the medium from type cultures, with 5% of defibrinated
sheep blood, after incubation at temperature of 35 ± 2°C and observed after 24 hours.
Microorganisms
Growth with 5% sheep blood
Hemolysis
Staphylococcus aureus ATCC 25923
Good
Beta
Streptococcus pneumoniae ATCC 6305
Good
Alpha
1
LABORATORIOS CONDA, S.A.
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Streptococcus pyogenes ATCC 19615
Good
Escherichia coli ATCC 25922
Good
Beta
BIBLIOGRAPHY
Altwegg. 1999. In Murray, Baron, Pfaller, Tenover and Yolken (ed.), Manual of clinical microbiology, 7th ed. American Society for
Microbiology, Washington, D.C.
Snavely and BrahierA. J. Clin. Path. 33:511. 1 960.Hosty, Freeman and Irwin, Public, Health. Lab, 1953.
STORAGE
25ºC
Once opened keep powdered medium closed to avoid hydration.
2ºC
2
LABORATORIOS CONDA, S.A.
www.condalab.com