U N I V E R S I T I E S S PAC E R E S E A R C H A S S O C I AT I O N DSLS Holiday Newsletter Passing the Leadership Torch at DSLS adopted by USRA’s Board of In an official ceremony on Trustees, thanking Adrian for November 17, 2010, the his highly successful service, leadership torch for the and presented him with an Division of Space Life official USRA baseball hat. Sciences was passed from Adrian also received a poster Adrian D. LeBlanc, PhD, of the International Space to Neal R. Pellis, PhD. Station signed by many of Adrian served as the fourth his employees and NASA Director of the division management. since 2002. A graduate of Neal R. Pellis, PhD the University of Massachu- Neal Pellis now serves as setts, Iowa State University, the fifth Director of the Division of Space Life Adrian LeBlanc, PhD Sciences. Prior to joining USRA, he was Senior Scienand the University of Kantist in the Space Life Sciences Directorate at NASA sas, he is a Professor in the Department of Medicine JSC, working on strategic planning, alliances, and at Baylor College of Medicine and an Adjunct Propartnerships to acquire basic and applied science and fessor in the Department of Orthopedic Surgery at technology for exploration the University of Texas “Leadership is understanding people and involving them to missions, and to manage Medical Branch. His prihelp you do a job. That takes all of the good characteristics, risks to crew in space and mary research interests relate to the investigation like integrity, dedication of purpose, selflessness, knowledge, on planetary missions. of the loss and recovery of skill, implacability, as well as determination not to accept Dr. Pellis received his PhD bone and muscle tissue in failure”. ~ Admiral Arleigh A. Burke in Microbiology from Miweightlessness and the deami University, Ohio and velopment of countermeasures. Thanks to his leader- was awarded a postdoctoral fellowship in Microbioloship, the division more than quadrupled its revenue gy at Stanford University, Palo Alto, CA. At the Uniduring his directorship. Adrian continues to bring to versity of Texas M. D. Anderson Cancer Center, he the DSLS considerable experience with both ground- then directed the Surgical Immunology Laboratory in based and in-flight experiments and now serves as an the Department of Surgical Oncology. Assistant to USRA’s Vice President of Science, Dr. Neal joined NASA JSC in 1994 and led the biotechDon Kniffen. nology cell science program until 2003. After a 16 months assignment to NASA Headquarters as International Space Station Program Scientist, he served as Associate Director of the Biological Sciences and Applications Office, working on exploration cell science, and was then appointed Senior Scientist in Space Life Sciences. Neal has had adjunct appointments at the University of Texas M. D. Anderson Cancer Center, the University of Texas Health Science Center at Houston Graduate School of Biomedical Sciences, the Mabee Laboratory of Biochemical and Genetic Engineering, Rice University, and the University of Texas Medical Branch Graduate School of Biomedical Sciences. L—R: Drs. Steve Mackwell, Don Kniffen, Adrian LeBlanc, and Neal Pellis. (Photo courtesy of Claudia Quintana.) We extend our heartfelt thanks to Adrian and a warm During the ceremony, Dr. Kniffen read the resolution welcome to Neal. 2 0 1 0 HO L I D A Y S Page 2 DSLS Welcomes New Employee Bill Todd Bill Todd joined USRA-DSLS in early November 2010. He works as Program Manager for Exploration Analogs at NASA Johnson Space Center and is responsible for leading simulations for NASA Astronaut and Flight Control Team training. He has also worked as an Operations Lead at the Mission Control Center in Moscow, Russia, and was the training lead for many Space Shuttle flights. In 2000, Bill identified the need for a different type of training and research for NASA and developed the NEEMO analog (“NEEMO” – NASA Extreme Environment Mission Operations). Several times a year, Bill is responsible for managing this undersea research Team. NASA utilizes the “Aquarius” undersea habitat as a research analog for space missions to develop concepts for long-term space habitation and has successfully completed 15 missions with a wide variety of objectives, including astronaut training, habitability, life sciences, engineering, tele-medicine and telehealth. Bill was an aquanaut on the 2000 “NASA SEATEST” mission and then again for the Oct. 2001 NASA NEEMO 1 mission. NEEMO missions, which are currently focused on addressing NASA’s exploration objectives, have been featured in a wide variety of media and have performed live educational outreach to millions of students. As a child, Bill grew up watching the Apollo launches from his beachfront hometown of Cocoa Beach, FL, where his father was employed in the Space program. It was there that he was immersed in and captivated by both sea and space environments. Bill Todd Bill’s influences have come from a broad field including Homer and Homer Simpson, Jacques Cousteau, Scott Carpenter, Jules Verne, Charles Darwin, Thomas Jefferson, Jimmy Page, SRV and his dad. Bill is a PADI Divemaster with 1000+ dives and an instrument-rated and seaplane pilot. He serves on the advisory board for the International Association of Handicapped Divers and is an Explorers Club Fellow and ab member of the SeaSpace Symposium. His hobbies include flying, lawn work, avoiding seafood, boating, diving, snow skiing and playing guitar in his rock band, “The Rockit Scientists”. Bill and his wife Karen have two daughters, Kristen and Kari. His third child is his red 1964 amphicar. They all reside in Seabrook, Texas. Bill has his office in Bldg 1 and can be reached at (281) 483-9825 or via william.l.todd@nasa.gov. Please join us in welcoming Bill to the DSLS team! Honors and Awards Megumi Hada received, with A. G. Georgakilas, the 2010 Terashima Award from the Japan Radiation Research Society for the paper: Hada M, Georgakilas AG (2008) Formation of clustered DNA damage after high-Let irradiation: A review. JRR 49(3), 203 -210. Megumi Hada at the Terashima Award presentation Christian Otto was appointed in Kyoto, Japan NASA HRP Research Lead for the IntraCranial Pressure/Vision Risk. Christian Otto, Lori Ploutz-Snyder, and Camille Shea received a NASA Recognition of Excellence award for their 2 0 1 0 HO L I D A Y S Christian Otto, MD contributions to the Open Innovation Service Provider Pilot Program. Minli Wang received a Scholars In Training (SIT) Camille Shea, PhD travel award for the 56th Annual Meeting of the RaMinli Wang, MD diation Research Society, Maui, Hawaii. Virginia Wotring was appointed Adjunct Assistant Professor in the Department of Pharmacology & Toxicology, University of Texas Medical Branch Galveston. Ginger was also appointed Adjunct Assistant Professor, Department of Health and Human Performance, University of Ginger Wotring, PhD Houston. Page 3 Grant Proposals Submitted Seidler R, Bloomberg JJ, Mulavara A, Wood SJ, “Spaceflight Effects on Neurocognitive Performance: Extent, Longevity, and Neural Bases,” submitted Step-2 proposal, NNJ10ZSA003N. Ajit Mulavara, PhD Ploutz-Snyder L, Scott J, Ploutz-Snyder R, Everett M, English K, Martin D, “Comprehensive Morphological and Functional Muscle Assessment for Long-Duration Spaceflight,” submitted Step-2 proposal, NNJ10ZSA003N. Ryder J, Ploutz-Snyder L, Wu H, “Effects of Radiation on the Effectiveness of Exercise Countermeasures to Protect Skeletal Muscle Function During Hindlimb Unloading,” submitted Step-2 proposal, NNJ10ZSA003N. Byerly D, Arndt D, Sognier M, “Emergency Dental Care and Repair for ISS/Exploration Missions,” submitted for NASA Internal Research and Development Award. Byerly D, Arndt D, Sognier M, “PEMF: Bone Loss Treatment for ISS/Exploration Missions,” submitted for NASA Internal Research and Development Award. Byerly D, Arndt D, Sognier M, “Innovative Composites Enabling Emergency Tooth Repair for ISS/Exploration,” submitted for NASA Internal Research and Development Award. Byerly D, Arndt D, Sognier M, “Noninvasive Countermeasure for Skin Disorders for ISS and Exploration,” submitted for NASA Internal Research and Development Award. Marguerite Sognier, PhD Byerly D, Arndt D, Sognier M, “Super Shield-Effective Radiation Protection for Humans Using Superconducting Magnets,” submitted for funding through NASA Innovative Research and Development. Byerly D, Arndt D, Putcha L, Sognier M, “Microwave-Mediated Nanoparticle Drug Delivery for Exploration Missions,” submitted for funding through NASA Innovative Research and Development. Byerly D, Sognier M, “Microwave Wand-Effective Elimination of Contaminants and Treatment of Skin Infections,” submitted for funding through NASA Innovation Charge Account (ICA) FY 11. Westby C, Platts S, Zwart S, Ploutz-Snyder R, Martin E, Sharina I, “Regulation of Nitric Oxide Mediated Vascular Function following Fractionated Low-Dose Space-Like Radiation Combined with Modeled Microgravity,” submitted Step-2 proposal in response to the NSBRI call for NRA NNJ10ZSA003N. Chris Westby, PhD Shi S-J, Wotring V, Lee, S, Stenger, M, Platts S, “Preventing Promethazine-induced Orthostatic Intolerance,” Step-1 proposal submitted, NNJ10ZSA003N. Wotring V, Wood S, Reschke M, “Development of a Benzodiazepine Antagonist for Mitigation of Promethazine-associated Side Effects,” Step-1 proposal submitted to the NASA call for NRA NNJ10ZSA003N. Zanello S, “Epigenetic basis for the cytoprotective mechanisms of the radioadaptive response in the mouse retina,” letter of intent for individual research grant submitted to the Foundation Fighting Blindness, Basic Research, Cell and Molecular Mechanisms of Retinal Disease (CMM). Smith SM, Zwart SR, “Sulfur-containing amino acids and bone health,” submitted to the 2011 International Council on Amino Acid Science Research Funding call for proposals. Scott Wood, PhD Smith SM, Zwart SR, Pierson D, Mehta S, Sams C, Crucian B, “Innate immune function and iron status during long-duration spaceflight,” Step-1 proposal submitted to the NASA call for NRA NNJ10ZSA003N. Smith SM, Kloeris V, Perchonok M, Zwart SR, “Multi-nutrient Nutrition Countermeasures,” submitted to the JSC Center-Level IR&D FY2011 Request for Proposals,. Smith SM, Zwart SR, “Management of Iron Overload during Long-Duration Spaceflight,” submitted to the JSC Center-Level IR&D FY2011 Request for Proposals. 2 0 1 0 HO L I D A Y S Sara Zwart, PhD Page 4 New Publications by DSLS Scientists Chappell LJ, Whalen MK, Gurai S, Ponomarev A, Cucinotta FA, Pluth JM (2010) Analysis of flow cytometry Lori Chappell, MS DNA damageresponse protein activation kinetics following X rays and high energy iron nuclei exposure. Radiation Research, in press, September 2010. Cook SB, Druger M, Ploutz-Snyder LL (2010) Scientific literacy and attitudes towards American space exploration among Lori Ploutz-Snyder, PhD college undergraduates. Space Policy (accepted with revisions), September 2010. Miller CA, Peters BT, Brady RR, Cucinotta FA, Hu Richards JR, Ploutz S, Schwadron NA, -Snyder RJ, MuKozarev K, Townlavara AP, Bloomsend LW, Kim Mberg JJ (2010) HY (2010) Space Rob Ploutz-Snyder, PhD Shaowen Hu, PhD Changes in toe radiation risk limclearance during its and EarthMoon-Mars environmental models. treadmill walking after longSpace Weather 8, in press, October duration spaceflight. Aviat Space Environ Med 81(10):919-28. 2010. Kim M-HY, De Angelis G, Cucinotta FA (2010) Probabilistic Assessment of Radiation Risk for Myung-Hee Kim, PhD Astronauts in Space Missions. Acta Astronautica (2010), http://dx.doi.org/10.1016/ j.actaastro.2010.08.035 Plante I, Ponomarev AL, Cucinotta FA (2010) 3-D visualization of the stochastic patterns of Ianik Plante, MD PhD the radial dose in nanovolumes by a Monte-Carlo simulation of HZE ion track structure. Radiation Protection Dosimetry (accepted), September 2010. Artem Ponomarev, PhD 2 0 1 0 HO L I D A Y S Singh S, Wu W, Zhang L, Klammer H, Wang M, Iliakis G (2010) Widespread dependence of backup NHEJ on growth Minli Wang, MD state: Ramifications for the use of DNA-PK inhibitors. International Journal of Radiation Oncology, Biology, Physics, in press, September 2010. Smith SM, Zwart SR, McMonigal K, Huntoon C (2010) Thyroid status of Space Shuttle crewmembers: Effects of Sara Zwart, PhD iodine removal. Aviat Space Environ Med, accepted October 2010. Zwart SR, Booth SL, Peterson JW, Wang Z, Smith SM (2010) Vitamin Zsuzsa SM, K status in spaceflight and groundDimmock JA, Ploutz-Snyder based models of spaceflight. JourR, Abdul-Malak nal of Bone and Mineral Research, Y, Leontieva J, accepted October 2010. Canfield K, BatAjit Mulavara, PhD ki SA (2010) PrePoster Printer dictors of smoking severity in patients with schizophrenia and alco- Did you know that a 60” color hol dependence. Journal of Addic- poster printer is available to you at tive Diseases, accepted September USRA Houston? The library also 15, 2010, AGA 2010-0055.R1. provides a large work surface and Jones LW, Liang L, Pituskin E, Battaglini CL, Scott JM, Hornsby WE, Haykowsky MJ. (2010) Effect of Exercise Training on Cardiorespiratory Fitness in Patients with Cancer: A Meta Analysis. The Oncologist, accepted October 2010. Jess Scott, PhD cutting tools so you can trim your poster to just the right size. Reservations are required with at least three (3) days lead time. To make a poster printer reservation, please contact Donna Sims at 281-486-2192 or via email at dsims@hou.usra.edu to work out a date and time that will work with both Donna’s and your schedule. Page 5 SPOTLIGHT ON: Virginia Wotring, PhD! step-children, now grown. My husband is a chef, currently back in school improving his credentials. We share our home with three dogs: a Labrador who likes to take me out to play, a shepherd mix who guards us all, and a Mastiff puppy who prefers to lie on the sofa with her head on a pillow. Our hobby is brewing our own beer; in fact, this mutual hobby is how we met. * What are you presently working on at JSC? I have just completed the new Pharmacology Evidence Book, a comprehensive literature review of everything known about medication * Tell us briefly about yourself and use during spaceflight. I now have the green-light to move on to your family. designing the new research plan for I came to JSC 15 months ago to our group. take over as the new Discipline Lead for Pharmacology. I have a * What is really important to you BS in Chemistry form Florida regarding your work at JSC? State, and a PhD in Pharmacology Being in a position to advance my branch of science to a new place is and Physiology from Saint Louis University. I am married with two very exciting for me, especially Virginia “Ginger” Wotring, PhD joined USRA in August of 2009 and now works in the Pharmacology Lab at JSC. It is the Ginger Wotring, PhD lab’s aim to mitigate pharmacotherapeutic risk by identifying and providing safe and effective diagnostic tools, pharmaceutical preparations, therapeutic procedures, and intervention strategies to enable successful space medical operations. Recently, Ginger agreed to be interviewed for this edition’s Spotlight Article. since that new place is actually off our planet. I am still a little amazed that all of us in space life sciences have the same ultimate goal, to improve crew health. I came to JSC from academia, where every lab tends to work in isolation towards its own goal. * What are your goals for this year? My goal for the coming year is to get some of my new ideas turned into funded and active projects. There is so much more to be learned about how medications work during spaceflight! Thank you so much for being our Spotlight Scientist for this edition of the DSLS newsletter, Ginger! Call for Papers! The library at USRA Houston has launched a new revolving exhibit featuring recent scientific papers by USRA employees. So when one of your publications has been accepted, please be sure to send a copy to either morgan@dsls.usra.edu or nute@dsls.usra.edu to be included in this new display! Currently, the exhibit features papers by Johnny Conkin, Myung Kim, Rob Ploutz-Snyder, Ajit Mulavara, James Fiedler, and Megumi Hada. Grant Writers’ Seminar Coming to USRA in early 2011! A seminar and workshop entitled “Write Winning Grants” will be held at USRA on February 16, 2011. This day-long event will comprehensively address both practical and conceptual aspects that are important to the grant proposal-writing process. Participants are taught to write with a linear progression of logic, beginning with a special emphasis on the 2 0 1 0 HO L I D A Y S Specific Aims/Objectives page as the key to the rest of the proposal. All participants will receive an extensive PowerPoint handout as well as a copy of The Grant Application Writers’ Workbook. Watch your Inbox for an email in early January and be sure to enroll! Page 6 Recent Scientific Presentations 56th Annual Meeting of the Radiation Research Society, September 25-29, 2010, Maui, Hawaii Hada M, George K, Cucinotta FA. The biological effectiveness of different radiation qualities for the induction of chromosome damage in human lymphocytes. Poster presented by Hada. Hada M, Zhang Y, Feiveson A, Cucinotta FA, Wu H. Distribution of low- and Megumi Hada, PhD high-LET radiation induced breaks in chromosomes are associated with inter- and intrachromosome exchanges. Poster presented by Wu. ribose) polymerase protects neural cells from clustered DNA damage induced by ionizing radiation. Poster presented by M Wang. International Congress of Medicine in Space and Extreme Environments, Berlin, Germany Otto C. Risk of microgravity-induced visual alterations and increased intracranial pressure. Presentation to Russian colleagues during the Biomedical Subcommittee Meeting of the International Joint Working Group. Patel ZS, Grugan KD, Rustgi AK, Cucinotta FA, Huff JL. Effects of ionizing radiation on stromal-epithelial communication in esophageal carcinogenesis. Poster presented by Patel. LaVerne J and Plante I co-chaired symposium, “Bridging the gap between track structure and stable end products.” Zarana Patel, PhD Plante I and Cucinotta FA. MonteCarlo simulation of radiation tracks and calculation of dose deposition in nanovolumes. Oral talk and poster presented by Plante. Otto C. Invited co-chair for New Medical Technologies Session. Ianik Plante, MD PhD Ponomarev AL, Cornforth MN, Loucas BD, Cucinotta FA. A Monte-Carlo model for the formation of radiation-induced chromosomal aberrations. Oral talk and poster presented by Ponomarev. Iliakis G, Singh S, Wang M, Staudt C. Delayed formation of DNA double strand breaks within clustered-damage sites from thermally unstable deoxyriArtem Ponomarev, PhD bose adducts in irradiated cells. Poster presented by Iliakis. Tang X, Wang M, Tian L, Renegar JR, Chris K. Wang CK, Wang H. Interaction of PARP-1 and CDK5 in DNA damage response in neural cells following ionizing radiation. Poster presented by H. Wang. Otto C. South Pole Station: An Analogue for human performance during long duration missions to the Moon and Mars. Oral presentation. Otto C. Breaking down the Mars communication barrier: Asynchronous guidance of a non-surgeon crew medical officer performing a laparoscopic appendectomy. Oral presentation. Otto C. Enabling telehealth technologies in support of Mars Exploratory Class Missions: Lessons from the Amundsen-Scott South Pole Station. Oral presentation. Otto C. Managing Exploration Mission Medical Events from a “Medical Mission Control”: The Mount Everest Medical Operations Expedition. Oral presentation. Space Radiation Journal Club Minli Wang, MD Wang M, Tian L, Tang X, Wang H. Poly (ADP2 0 1 0 HO L I D A Y S Dr. Ushakov, IBMP, Dr. Otto, USRA, Barbara Corbin, NASA Ponomarev A. A generalized chromosomal aberrations model predicting the distribution of small deletions and associated small DNA rings produced by the (cont. on pg. 8) Page 7 Recent Presentations (cont.) restitution of DNA double-strand breaks induced by ionizing radiation in human cells concurs with the novel experimental technique that detects small deletions. NASA JSC. Applied Life Sciences, Department of Kinesiology and Community Health, October 15, 2010, UrbanaChampaign, Illinois. Also met with student groups and toured faculty labs. Huff J. Influence of ionizing radiation on stromal-epithelial communication in esophageal carcinogenesis. NASA JSC. Wotring V. Liver Function in Mice After Radiation Exposure, Invited talk for the HCAD Core Lab Open House, September 17, 2010, NASA JSC. Wang, M. High LRT radiation sensitizes TGFb-mediated epithelial to mesenchymal transition (EMT). NASA JSC. Zanello S. RNA-DNA storage at room temperature, presented to JSC Deputy Center Director, Ellen Ochoa. Janice Huff, PhD Zanello S. Effects of space Environmental Factors on the Mouse Visual System: Radiation. Invited talk for the JSC Core Lab Open House, NASA JSC. Chappell L. Astronaut chromosome aberrations and cancer risk. October 29, 2010, NASA JSC. Presentations Elsewhere Johnny Conkin, PhD Susi Zanello, PhD Conkin J. Estimated P(DCS) from Zanello S. RNA-DNA storage at room temperature. venous gas emboli. NESC Review Invited talk for the JSC Technology Working Group, of V-5 Prebreathe Protocol, October NASA JSC. 14, 2010, USRA Houston. Otto C. Testing NSBRI Smart Med- DSLS Brown Bag Seminars ical Systems in Extreme Exploration Westby C. Spaceflight: Understanding the Impact on Vascular Function. Environments. Invited presentation, NSBRI Smart Medical Systems & Technology Team Retreat with September 23, 2010, USRA Houston. NASA Exploration Medical Capabilities Team. USRA Professional Ploutz-Snyder L. Evidence based strategy for the design of exercise programs: Cardiovascular, skeletal muscle and bone health during long duration spaceflight. Invited talk, University of Illinois, College of Visitor from Japan Development Series Ploutz-Snyder, R, invited talk. OLS Regression (cont.), SMAR, USRA Houston. Rob Ploutz-Snyder, PhD On behalf of the NASA JSC Space Radiation Program Element, DSLS invited Kayo Yoshida, PhD, to conduct collaborative research at NASA JSC and Brookhaven National Lab. Drs. Megumi Hada and Kayo Yoshida 2 0 1 0 HO L I D A Y S Dr. Yoshida is an associate professor in the Department of Molecular Genetics at Osaka City University’s School of Medicine. Her main research interests are the effects of space radiation on mouse ES and iPS cell development. While in the US, Dr. Yoshida worked closely with Megumi Hada, PhD, to learn new techniques to analyze chromosome aberrations. DSLS’ Minli Wang, MD, graciously hosted Dr. Yoshida for the greater part of her visit. Drs. Kayo Yoshida and Minli Wang A return visit is already being planned for April 2011. また近いうちにお会いしましょう! Page 8 News from Other USRA Divisions NASA reported in a press release dated December 2, 2010 that the Stratospheric Observatory for Infrared Astronomy (SOFIA) completed the first of three science flights to demonstrate the aircraft's potential to make discoveries about the infrared universe. feed and grow. This premiere science flight took off from an Air Force runway in Palmdale, California, on Nov. 30, and flew for approximately 10 hours. NASA's Ames Research Center in Moffett Field, CA, manages the SOFIA science and mission operations in cooperation with the Universities Space Research Association and the Deutsches SOFIA Institut at the University of Stuttgart, Germany. Congratulations to our colleagues at Ames! SOFIA (Image Courtesy of NASA) The airborne observatory is an international collaboration between NASA and the German Aerospace Center, Deutsches Zentrum für Luft- und Raumfahrt (DLR). Infrared Image of the Center of the Orion Star Complex taken by the FORCAST mid-infrared Camera on board SOFIA. SOFIA is a heavily modified Boeing 747SP that (Image Courtesy of NASA) cruises at altitudes between 39,000 and 45,000 feet. It will allow researchers to better understand a wide For additional SOFIA images, please visit http:// range of astronomical phenomena including how stars www.nasa.gov/mission_pages/SOFIA/multimedia/ and planets are born, how organic substances form in imagegallery/index.html interstellar space, and how supermassive black holes And News Here on Earth, Closer to Home ... On October 13, 2010, Kay Nute became a grandma again: Caroline Grace was born at 11:28 am, weighing in at 9 lbs 6 oz. and measuring 21.5 inches. Introducing Ms. Caroline Grace Mulac 2 0 1 0 HO L I D A Y S Baby Girl, Mom Lindsey, and Grandma Kay are doing well, and Big Brother Adam likes her too! Jancy McPhee leads the efforts for the International Youth Art Competition in conjunction with next year’s IAA Humans in Space Symposium. Children from around the world between the ages of 10 and 17 are being challenged to artistically express the symposium’s theme of "Integration and Cooperation in the Next Golden Age of Human Space Flight". For a wonderful sneak preview, please visit http://www.youtube.com/watch?v=3mMGXvucFs&feature=youtube_gdata_player, a submis- Proud Grandma Kay sion from an elementary school in Turkey (since it is already public, it is permissible to share). Page 9 Happy Holidays! The DSLS Administrative Team wishes you and your family peace and joy for the holidays and throughout the New Year. The DSLS Newsletter is published on a bi-monthly basis. Dagmar Morgan—Editor Division of Space Life Sciences Universities Space Research Association, 3600 Bay Area Boulevard, Houston, TX 77058 2 0 1 0 HO L I D A Y S Page 10