DISCIPLINE OF MICROBIOLOGY
SCHOOL OF NATURAL SCIENCES
SAFETY STATEMENT
In compliance with Part 4 of the NUI,Galway Safety Statement to be found at this web link
http://www.nuigalway.ie/healthsaf/index.php?menu=3&page=22
2013
LIST OF CONTENTS
SECTION
Title Page and List of Contents
0
Safety Statement Declaration and Signature
0
Room Identification and Named Person Responsible
1
Hazard Control, Approved Handling Procedures and SOP’s
2
Risk Assessment of Chemical Agents Title Page
3
List of Chemical Agents Assessed as Low Risk
4
Sample of Chemical Agents Assessed as Low Risk
5
Risk Assessment of Biological Agents Title Page
6
List of Biological Agents Assessed
7
Biological Agents Assessments
8
Risk Assessment of Equipment and Procedures Title Page
9
List of Equipment and Procedures Assessed
10
Equipment and Procedures Risk Assessment
11
Key to show how Hazard Rating Numbers are derived
12
Safety, Health and Welfare at Work Act, 2005
Safety Statement Declaration of the Discipline of Microbiology,
National University of Ireland, Galway
To each employee, student and visitor to the Discipline of Microbiology:
The head of the Discipline of Microbiology, of the National University of Ireland, Galway, (hereafter called the discipline),
acknowledges his/her responsibility to co-operate with the President and Governing Authority of the University in
implementing the statutory obligations of the Safety, Health and Welfare at Work Act, 2005 (hereafter called the Act) and any
Regulations made there under.
Microbiology is committed to providing, so far as is reasonably practicable, a safe and healthy work environment for all
sectional employees and to meet its duties to students and authorised visitors who may be affected by this disciplines
operations. In accordance with Section 20 of the Act, this sectional safety statement identifies the hazards and assesses the
risks of all sectional activities; it details the protective and preventive measures taken, personnel responsible, relevant
resources, and employee co-operation required to secure the safety, health and welfare at work of persons employed in this
discipline. The head of discipline will consult with staff on safety, health and welfare at work matters, including this
document, in accordance with Section 26 of the Act.
This Microbiology Safety Statement will be annually reviewed in the light of experience and developments within this
discipline.
Signed:
_______________________________________
Head of Microbiology
Date:
________________________________________
National University of Ireland, Galway.
Discipline of Microbiology Room Locations
Site:
ARTS SCIENCE BUILDING N.U.I.,Galway
Date:
Audit carried out by:
24th May 2013
Professor James O’Gara
Ground Floor Microbiology Room Numbers
Room No.
Name Plate /Identification
101
102
103
104
105 A
105 B
106
107
108
109
110
111
112
113
114
115
116 (a) (b)
117
118
119
120
121
100P
126
127
Dr. P.R. Smith(Retired)
Dr. G. Fleming(Office)
Dr. G. Wall (Office)
Dr. T. Barry(Office)
S.I.P.-F.I.S.H.
Dr. C. O’Byrne(Office)
Fermentation Lab
Fume Cupboard Room
Instrumentation Room ( Containment Lab )
Mr. W. J. O’Connell(Retired)
Store
Molecular Diagnostics
Rec. Protein Lab
Bacterial Stress Response Group
Microbial Oceanography
Library / Common Room
Prep Room (a) Containment Lab (b)
now part of 118
Instrument Room (Genomics suite)
now part of 118
Cold Room / Freezer
Hot Room
Third Year Teaching Lab
Store
Prof. L.K. Dunican Theatre
Person Responsible
Dr. G. Fleming
Dr. G. Wall
Dr. T. Barry
Dr. Gavin Collins
Dr. C. O’Byrne
Dr. Tom Barry/ Dr. C. O’Byrne
Dr. G. Fleming
Dr. A. Boyd
Mr Maurice Martyn
Mr Maurice Martyn
Dr. Tom Barry
Dr. G. Wall
Dr. C. O’Byrne
Dr. G Fleming
Prof. James O’Gara
Prof. James O’Gara (a) Dr A. Boyd (b)
Prof. James O’Gara
Prof. James O’Gara
Prof. James O’Gara
Prof. James O’Gara
Prof. James O’Gara
Prof. James O’Gara. / Buildings Office
First Floor Microbiology Room Numbers
Room No.
Name Plate /Identification
Person Responsible
201
202
203
204/205
205
206
207
208
209
210
Board Room
Office/Secrectary
Prof. James O’Gara
Functional Environmental Laboratory
Now part of 204
Dr Gavin Collins
Dr. A. Boyd (Office)
Dr. F. Abram (Office)
Dr. C. Carroll (Office)
Technical Staff (Office)
Prof. James O’Gara
Ms. C. O’Connell
Prof. James O’Gara
Dr Florence Abram
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
Dr Gavin Collins
Dr. A. Boyd
Dr. F. Abram
Dr. C. Carroll
Ms Ann Smyth, Mr Mike Coughlan,
Dr. Katrina Lacey and Mr Seamus Dunne
Centrifuge (Photo Copy) Room
Prof. James O’Gara
Ecophysiology
Dr. G. Collins
Infection Biology
Prof. James O’Gara
Pathogenic Mechanisms
Dr. A. Boyd
Enteric Pathogen Research Laboratory
Dr. C. Carroll
Technical Support
A. Smyth, M. Coughlan, K. Lacey S. Dunne
Preparation Room
Prof. James O’Gara
Toilet
Buildings Office
Toilet
Buildings Office
Dark room
Prof. James O’Gara
Cold Room
Prof. James O’Gara
Hot Room 30o
Prof. James O’Gara
o
Hot Room 37
Prof. James O’Gara
Cold Room
Prof. James O’Gara
Large Teaching Lab
Prof. James O’Gara
Tyndal Theatre
Buildings Office
Center for Environmental Science Ground Floor
Room No.
Name Plate /Identification
Person Responsible
ES101
ES102
ES103
ES104
ES105
ES106
ES107
ES108
ES109
ES110
ES111
146 BIO Wing
147 BIO Wing
140 BIO Wing
Fridge Freezer Room
Pilot Lab
Microbial Ecology Lab
Marine Micobial Ecology
Microbiology Seminar Room
Dr. C. Smith
Prof. V. O’Flaherty
Research Office
Instrument Room
Constant Temperature Room
Biochem Communications Room 2
Post Doc.
Post Doc.
Higher Diploma Lab.
Prof. V. O’Flaherty
Prof. V. O’Flaherty
Prof. V. O’Flaherty
Dr. C. Smith
Prof. James O’Gara
Dr. C. Smith
Prof. V. O’Flaherty
Prof. V. O’Flaherty
Prof. V. O’Flaherty
Prof. V. O’Flaherty
ISS
Prof. James O’Gara
Prof. James O’Gara
Dr G. Fleming, Dr. C. Carroll
Centre for Environmental Science First Floor
Room No.
Name Plate /Identification
Person Responsible
ES201
ES202
ES203
ES204
ES205
ES206
ES207
ES208
ES209
ES210
Environmenmtal Sciences Seminar Room
Environmenmtal Sciences Under Grad Lab
Dirty Support Room
Applied Ecology Unit
Entrance to Main Teaching Laboratory( 225 )
Dr. Caitríona Carlin, Dr. Gesche Kindermann
Dr. M. Gormally Ms. A. Travers-Lynagh
Analytical Room
Environmenmtal Sciences Computer Suite
Store
Dr.
Dr.
Dr.
Dr.
M. Gormally
M. Gormally
M. Gormally
M. Gormally
(office)
(office)
Dr. M. Gormally
ISS / Dr. M. Gormally
Dr. M. Gormally
OUTSIDE FACILITIES MICROBIOLOGY
Room No.
Name Plate /Identification
Person Responsible
NONE
NONE
Bottled Gas Storage Shed
Three storage units for field equipment.
Prof. V. O’Flaherty.
Prof. V. O’Flaherty, Dr. M. Gormally, Dr Gavin Collins
HAZARD CONTROL, APPROVED HANDLING PROCEDURES
AND STANDARD OPERATING PROCEDURES
This Section was compiled by Dr. Cyril Carroll
Included in Document :
Laboratory Hazard Control :
Hazard Control :
Hazard Control :
Hazard Control :
Infrastructural Safety Precautions
Emergencies
Safe Laboratory Techniques
Good Microbiological Techniques
Waste Disposal
Appendix 23c - Approved Handling Procedure - Sharps Material
Appendix 23d - Approved Handling Procedure - Biohazard Material
Sop #1.
Safe Operating Procedure Avoiding The Production Of Biological Aerosols
Sop# 2
Sop #3. :
Sop #4a.
Sop #4b.
Sop #5
Biological Waste Disposal
Accidents, Spills, And Emergencies
Minor Biological Spill Inside Biological Safety Cabinet.
Minor Biological Spill Outside Biological Safety Cabinet.
Extensive Biological Spill (Significant Risk ) Inside Lab And Outside Biological Safety
Cabinet
Biological Spill Outside Lab, During Transportation
Accident Treatment And Reporting
Working With Human-Derived Material With Specific Reference To
The Risk Of Needle Stick Injuries.
Operating Procedure For Biological Safety Cabinet
Emergency Wash Facilities
Guidance for Fieldwork and a sample fieldwork risk assessment form
Sop #6
Sop #12
Sop #13
Sop # 14 :
Sop # 16 :
Sop # 17 :
SECTION I - GUIDANCE
LABORATORY HAZARD CONTROL - INFRASTRUCTURAL SAFETY PRECAUTIONS
INTRODUCTION
Laboratory staff may be exposed to a wide range of chemical, physical and biological agents. These
may be harmful by inhalation, injection, ingestion or through skin or mucous membrane contact.
Precautions listed in this section provide simple and effective protection against these risks provided
they are rigorously implemented.
Each individual has a responsibility for both their own and their colleagues safety. Safety rules for
staff and visitors have been prepared and are available in this Safety Statement. (SEE APPENDIX 3a
for GENERAL SAFETY RULES ).
LABORATORY FACILITIES
The research laboratories in the Discipline of Microbiology (DOM) are suitable for work with
Category 1 and 2 organisms. The following code of practice, is recommended as a minimum for work
on such organisms.
BIOHAZARD COMMUNICATION
Laboratories which contain specific identified hazards must be clearly marked with warning signs.
Access to such areas is strictly limited to authorized personnel with the appropriate training and
expertise. For such areas prior authorization must be obtained from the research supervisor before
visitors or other unauthorized personnel are permitted to either enter the laboratory or undertake any
work within the laboratory.
All signs/labels are required to have the universal biohazards symbol and be fluorescent orange or
orange-red.
a. LABORATORY SIGNS
Biosafety level 1 and level 2 areas (using agents not known to cause human disease) should be
labeled at the entrance(s) with a sign identifying the area as a Biohazard Area and advising the
use of Universal Precautions.
b. CULTURE ROOMS
All rooms where biohazardous agents are cultured and grown must be labeled to identify the
entrance requirements and should follow the guidelines defined for Biosafety level & 2 areas.
c. STORED MATERIAL
The following defines the labeling requirements for containers of bio-hazardous materials:
1
Regulated waste containers - Biohazard label container (e.g. sharps container);
2
Refrigerator/freezer holding infectious agents - Biohazard label;
3
Containers used for storage or transport - Biohazard label container;
4
Individual specimen containers in active usage - No labels required, however, the multi-specimen
holder should have a biohazard label;
•
Contaminated equipment - Biohazard label and label specifying where contamination exists;
GENERAL LABORATORY SAFETY RULES
This is a code of practice that should be adhered to by all laboratory staff while working in the
laboratory. It is the responsibility of each member of staff to ensure the rules laid down are under
compliance.
Access (authorized) to research laboratories
Access to each individual research laboratory is strictly limited at all times to those individuals
authorised by the appropriate research supervisor or person in charge. In the case of visiting
researchers and new staff the research supervisor is responsible for ensuring that the appropriate safety
training is provided, if necessary by specialist safety consultants, before laboratory access is
authorised.
ACCIDENT REPORTS
Accidents & Dangerous Occurrences
All accidents to persons or damage to property must be reported to the Safety Office within 24 hours.
If serious - telephone immediately. The University Safety Office must be notified if any University
employee is away from work for 3 days or more as a result of an accident or illness which may be
related to their employment.
An Accident/Incident Report is completed for all hazardous incidents and “near misses” even if no
injury is caused. The NUIG Accident Report Book is located in the Chief Technicians Office in the
Microbiology . The Microbiology Chief Technical Officer keeps a copy of these reports on file in the
Safety File. (See also Sop # 2.) .
After Hours Working
After hours working must be restricted as far as possible, to minimise risks to safety. All persons
working outside normal hours must take special precautions to ensure their safety; receive approval
from their head of section; work in pairs; be familiar with fire safety and the relevant emergency and
first aid procedures.
Biohazardous Waste
(See also 'HAZARD CONTROL - WASTE DISPOSAL').
To reduce the risk of local contamination, all biohazardous waste must be properly contained,
chemically treated or autoclaved as required. Final disposal must be by an approved means. All
biohazardous waste must be kept separate from other wastes and properly labelled as to its hazard.
Chemical Waste
(See also 'HAZARD CONTROL - WASTE DISPOSAL').
All hazardous chemical waste must be fully labelled, giving the producers name[s], location, where
and when the waste was generated and the nature of the waste, in as much detail as possible. Details
of each container must be entered in a log book. Containers must be stored in a safe location prior to
collection by an approved contractor for final disposal. Toxic, flammable or corrosive waste must be
prevented from entering the drains. Details and advice are available from the University Safety Office,
ext. 2678.
DISINFECTANTS
(See also HAZARDCONTROL : DISINFECTANTS AND STERILIZATION)
The main disinfectants used in the laboratory is 70% Industrial Methylated Spirits ; IMS) or a
Hypochlorite solution (e.g. Virkon, Domestos, etc.).
EATING, DRINKING & SMOKING
Eating, drinking, smoking or the storage of food for human consumption in any laboratory is strictly
prohibited.
EMERGENCY PLANS
Consideration must be given to any hazardous operation which depends on electricity, water, or
ventilation, on the action to be taken in the event of the failure of such services. Also, the correct
action to be taken in case of spillage, fire or explosion must be pre-planned. (SEE SECTION
HAZARD CONTROL - EMERGENCIES)
ESCAPE ROUTES & DOORS
All escape routes must be kept free of obstructions and all doors on escape routes must be unlocked
and unbolted (except where approved automatic securing devices have been fitted) when a building is
occupied.
EYE AND FACE PROTECTION
1. All persons entering a laboratory, workshop or other area, where any operation or substance is in
use which could cause eye injury or where there is a risk of contamination with infectious or
caustic material, must wear suitable eye protection while in the area. Observe the local signs in
laboratory, workshop areas, etc. in respect of eye safety.
• Do not wear contact lenses when engaged in hazardous procedures unless protective goggles are
also worn.
• Eye wash stations are properly stocked with sterile eye wash solution and are located inside the
main laboratory door
• All staff members should be familiar with the use of the eye wash station .
FIRST AID
All injures, except the minor, should be referred to Casualty. The most common minor injuries include
burns/scalds, cuts and chemical splashes. They require immediate first-aid.
•
Burns/scalds - treat by immersion in cold water for 10 minutes.
•
Cuts - promote bleeding and rinse under cold running water; dry and apply dressing. All open
cuts and abrasions must be kept covered while working.
•
Splashes with harmful agents - flush affected area including eyes if affected, with copious
volumes of cold water for several minutes.
•
A first aid box is located in each laboratory . Know where it is. Advise senior staff of any
deficiencies.
•
Fill up Accident/Incident Report.
FOOTWEAR
• Sandals, open-toed shoes and excessively high heels are not recommended.
FUME HOODS
• Use the fume hood for weighing out chemicals, chemical handling operations , do not use for
storage as it effects its operation and adds to the risk of accidents.
• Before using the fume hood switch it on to its highest setting.
• Bring all the required equipment/chemicals to the cupboard, and place in the working chamber. Do
not block exhaust slots or openings.
•
•
•
•
•
•
•
•
•
If a significant volume of flammables is being used, use only flame-proof/spark-proof equipment.
In any event avoid the use of naked flames within the cupboard, as this will interfere with the
airflow.
Rotating machinery, e.g. centrifuges, will disturb the air-flow.
At all times when using the cupboard, reduce the sash opening to the minimum. This gives the
greatest level of containment and some degree of protection against splashes and minor
explosions/implosions.
Do not sit at a fume cupboard as this restricts your movements in an emergency.
Avoid rapid movements, especially of the arms within the fume cupboard.
Clean up any spillages promptly and effectively/efficiently referring to the MSDS and the
laboratory chemical spill kit.
Never leave any potentially hazardous experiment or process unattended.
When the experiment is completed allow the fan to run for at least 10 minutes to ensure that the
fumes have been removed before raising the sash.
Always tidy the fume hood at the end of an experiment. Return chemicals and equipment to
storage places. Remove all unwanted material. If necessary, decontaminate and clean the walls of
the chamber with large amounts water and absorbent tissue (disposed of either in normal bin or
chemical bin in shed).
Gas Cylinders
Gas cylinders must, at all times, be secured, by chains, straps or placed in suitable stands or trolleys.
Hazardous or flammable gas connections must be checked for leaks at regular intervals (at least once a
week). Where possible, cylinders should be stored outside and gas piped into the building. Where
specified, keep cylinders away from direct sunlight. Flash back arrestors must be fitted on all
flammable gas cylinders e.g. CO2, H2, Acetylene. Consult appropriate safety data sheet for further
safety information.
HAIR
• Hair (and beards) should be secured so that it does not come into contact with contaminated
materials or surfaces.
HOUSEKEEPING
• Do not obstruct passages or exits.
• Do not block access to fire safety equipment.
• Ensure bunsen burners do not create fire hazard; turn off when lab is unoccupied.
• Keep working area clean and tidy and wash benches daily with disinfectant.
• Mop up spillages immediately.
• Discard waste into appropriate containers; pay particular attention to sharps and contaminated
waste.
• Clean refrigerators, freezers, water baths and centrifuge regularly.
• Ensure floors are not a hazard. This is the responsibility of the supervisor in each section
IMMUNISATION
• Immunization / vaccination is offered as appropriate.
INDUCTION OF NEW STAFF
Staff new to the Microbiology or to a particular section, must be given a basic introduction by a senior
staff member before they begin working.
The induction must include:
(i)
Determination of health status including immunisation history
(ii)
An opportunity to read the Safety Statement and ask questions
(iv)
A tour of the area indicating fire exits and equipment
(v)
Indication of special hazards in the area
(vi)
Appropriate training in any procedures to be undertaken
Supervisors must ensure that temporary staff are included in the induction process.
JEWELLERY
Do not wear jewellery that can become entangled with moving equipment or hang into infective or
hazardous materials
LABELLING
Care must be taken to ensure that labels on all chemical containers, etc., are maintained in good
condition and where replacement containers are used, they must be suitable and the full safety
information must be transferred to the container. Chemicals with limited shelf life and in particular
chemicals which can form peroxides must be labelled with an expiry date.
MANUAL HANDLING
NUIG provide Manual Handling courses. Organise the work to minimise the amount of lifting
necessary, using mechanical means or other aids. When help is needed for lifting heavy or awkward
loads, get everyone to work together but make sure only one person gives clear, unhurried instructions,
provide protection for hands and feet, and protective clothing where necessary.
Make sure that everyone knows the correct lifting techniques:
ƒ
do not jerk and shove - twisting the body may cause injury
ƒ
lift in easy stages - floor to knee then from knee to carrying position. Reverse
this lifting method when setting the load down
ƒ
hold weights close to the body. Lift with the legs and keep the back straight
ƒ
grip loads with palms, not fingertips. Do not change your grip while carrying.
ƒ
do not let the load obstruct your view. Make sure the route is clear before
you start moving
NEEDLE-STICK INJURIES - See also Section - " Suggested Procedures for Working with HumanDerived Material With Specific Reference to the Risk of Needle Stick Injuries." - (SOP #13 )
IMMEDIATE CARE AFTER INOCULATION INJURY
•
Encourage bleeding.
•
Wash with antiseptic handwashing liquid
•
Cover with a waterproof dressing.
•
Splash into eye or mouth should be rinsed out with copious water or saline eye wash, available
beside main laboratory door.
•
Report accident to Safety Officer, then report to medical officer (University Health Centre).
Complete accident form.
•
Consult policy on inoculation injuries. (Health and Safety file)
•
Both source and exposed person should be encouraged with consent and counselling, to have
blood taken for Hep. B and H.I.V. tests.
•
FOLLOW UP AFTER INOCULATION
•
Hepatitis B hyperimmune globulin and or an accelerated Hep B vaccination course should be
administered in accordance with the document “procedures to follow in the event of an
inoculation incident.
•
Prophylactic zidovudine may be offered if the source is HIV positive. In accordance with the
document “procedures to follow in the event of an inoculation incident” this should be
commenced within 2 hours.
PIPETTING AND PIPETTE USAGE
Mouth pipetting of any material is prohibited. Pipetting aids are available for all tasks.
PLANNING
Before any experiment is initiated, the potential hazards must be fully evaluated and the appropriate
action taken to ensure that, as far as reasonably practicable, no hazard is likely to occur which would
injure the experimenter, other persons, or damage equipment, the building or the environment. The
safe and legal disposal of all chemical, biological or radioactive waste generated must also be
pre-planned.
PERSONAL PROTECTIVE EQUIPMENT (PPE)
Personal protective equipment (PPE) is designed to protect the worker from contact with biohazardous agents as well as protect the work from contamination by the worker. PPE is considered a
secondary line of defense against the infection of employees. The primary line of defense is the use of
Universal Precautions and good laboratory techniques.
PPE is selected on the basis of what parts of the body have the potential for exposure when working with a biohazardous
agent. Normal working conditions as well as accidental spills, breakage, or equipment malfunction must be considered in
this decision. The following is a general listing of the PPE that can be used to protect specific regions of the body.
Protective clothing must be worn when any operation involving hazardous substances or materials are
being used. Laboratory coats should conform to the Irish NISO specification (Howie type). All
protective coats and boiler suits must be removed before leaving the work area or entering a room
where food is eaten or stored. Protective clothing must be maintained in good condition and
washed/laundered as required. Where unguarded rotating devices are in use, no loose clothing or
jewellery is permitted and long hair must be tied back.
•
•
•
•
•
•
•
LABORATORY COATS
All members of staff must wear a Howie coat in the laboratory.
Coats should be removed when leaving the laboratory and never worn in the office area.
Coats should be clean and fastened correctly and changed regularly or when visibly
contaminated.
Authorised visitors should wear Howie coats in the laboratory area.
The elasticated cuffs should protect sleeves and jewellery adequately.
Coats should be stored in the correct areas (i.e. on the hooks provided, not on the back of chairs
or in lockers).
Plastic aprons should be available to wear over Howie coats if there is a risk of splashing.
GLOVES
•
•
•
•
•
•
All staff members should wear approved disposable gloves handling Class 2 bacterial
pathogens. See section 5.12d of the NUI, Galway Safety Statement
Contaminated gloves should be changed and disposed of into the biohazard waste bins
provided in the laboratory.
Non-latex and powder free gloves such as vinyl or nitrile are supplied for use.
Protective gloves are available for handling hot/cold objects.
Hands must be washed prior to entering the office area and prior to leaving the laboratory area.
An alcohol gel disinfectant is available beside the hand washing area.
In addition, Eye Protection and Respiratory Protection should be used when necessary
RADIOLOGICAL SAFETY
All matters pertaining to radioactive materials should be addressed to the DOM Radiological
Protection Officer (Dr. Conor O Byrne).
REFRIGERATORS AND DEEP FREEZERS
Refrigerators and deep freezers, if used to store flammable chemicals, must have spark proof switches
and thermostats..
SECURITY
Notify security if intruders are found; RING
•
•
•
3333 / 2198 or (999 / 112)
Valuables should be kept in a secure area ; it is not advisable to keep items of high value in the
workplace.
If the laboratory is being left empty during the day, the doors should be locked.
All windows and doors should be locked in the evening.
SHARP OBJECTS
- See Relevant Section: "Disposal of Laboratory Waste" - (Health Service Executive
InfectionControl Manual: Copy in health and Safety File).
Broken glass, needles, scalpel blades and other objects which could cause injury must be put into
“sharps containers” or glass/aerosol boxes, as appropriate, and never into ordinary waste bins.
See Appendix 23C for the ‘Safe Disposal of Sharps’ to try and avoid these injuries
SOLVENTS AND FIRE PRECAUTIONS
The quantities of flammable solvents and other hazardous chemicals stored internally must be kept as
low as reasonably practicable in all laboratories. The use of specially designed steel cupboards for the
storage of flammable materials is strongly recommended. Doors on such storage units must be
self-closing or kept closed at all times. Never store hazardous materials in access routes or near an
exit. Distillations must not be left unattended.
SOUND/NOISE
Sound levels above 85dB(A) must, if possible, be reduced at source by providing sound absorbing
enclosures, vibration control or other means. If this is not feasible, hearing protection must be
supplied and worn.
SPILLS
Spillage of cultures and chemicals and breakage of vessels containing them must be reported
immediately to the supervisor or safety officer. If the spillage is considerable, the room should be
vacated pending decontamination by Laboratory staff.
BIOLOGICAL SPILLS
Dealing with a biological liquid spillage on desk or in safety hood. Personnel are required to be
familiar with the MSDS (infectious substances) to particular pathogens which may be encountered in
the lab.
Any individual involved in the clean-up of a spill must determine the nature of the spill and the
appropriate clean-up procedure before beginning clean-up. (See SOP 3 -7).
•
•
A biological spill kit is available and may be used in accordance with the manufacturers
instructions.
Follow SOP 3-7, for relevant Spill type )
CHEMICAL SPILLS (See Appendix 19f - Emergency Action Plan - Chemical Spills)
Personnel are required to be familiar with the MSDS of the particular chemicals that they
encounter in the lab. A chemical spill kit is available and may be used in accordance with
manufacturer instructions.
• In the event of a spill please follow the appropriate protocols/cleaning method in accordance with
individual chemical requirements.
• Turn off ignition sources
• Ventilate room
• Clear area of people
• Identify spill source and apply appropriate chemical spill agent (see Chemical spill kit
instructions). At end of cleaning check pH.
Body spills: For phenol, chloroform, toluene, acetic acid and isoamyl alcohol, contact with eyes and/or
skin should be washed with large amounts of water immediately and medical help should be sought.
Toxic and Hazardous Chemicals
The use of hazardous chemicals must be organised so that the escape into the room air, of hazardous
vapour or particles is prevented as far as reasonably practicable. An efficient fume cupboard must be
used as necessary. The use of personal respirators should only be considered in an emergency or other
exceptional circumstance. Toxic chemicals must not be taken out of the Microbiology without the
specific permission of a member of the academic staff. Chemicals must never be stored in personal
lockers. Consult relevant safety data sheets for further safety information.
TRANSPORT OF SPECIMENS WITHIN LABORATORIES AT DOM
• Specimens delivered to Microbiology Dept. are transported to the Research
Laboratories in a closed transport box.
• Decontaminate the transport box on removing the specimens.
• (SEE SOP 6) for detailed procedures
TRAINING
Adequate training and instruction must be given, before any hazardous equipment or procedure is
commenced or a modified procedure is adopted.
UV LAMPS /LASERS
Suitable goggles must be worn when using UV lamps and lasers unless the equipment and emissions
are totally enclosed and effective interlocks fitted.
UNATTENDED EXPERIMENTS
Unattended experiments must be authorised by a member of the academic staff
VACUUM EQUIPMENT
Evacuated vessels, desiccators, vac-lines, etc., must have adequate protection to guard against injuries caused by implosion.
WASH HAND BASINS (WHB)
• WHB’s should have adequate supply of liquid soap, paper towels and handcream available.
• An alcohol gel disinfectant is also available beside the WHB.
• All staff members should wash their hands prior to leaving the laboratory area and after removing their disposable
gloves.
Working Alone. Supervision
No person may work on any potentially hazardous operation or in a hazardous environment, unless
another responsible person is constantly within easy calling distance or an approved signalling device
is in use. Undergraduates must be under the direct and constant supervision of a competent person
when working in laboratories or on machines or other equipment capable of causing injury.
SEE APPENDIX 3c FOR SAFETY PRECAUTIONS FOR:
• NON-SCIENTIFIC RESEARCH LABORATORY USERS
• VISITORS TO THE RESEARCH LABORATORIES
• CLEANING STAFF OF RESEARCH LABORATORIES.
• MAINTENANCE STAFF AND SERVICE ENGINEERS.
• PORTERING /SECURITY STAFF Of RESEARCH LABORATORIES.
HAZARD CONTROL : EMERGENCIES
(See also SOP #3: Accidents, Spills, and Emergencies).
EMERGENCY PROCEDURES FOR MICROBIOLOGICAL LABORATORIES
The specific requirements for emergencies due to Biohazardous aerosol release or spill will depend on
the type of experimental work, the nature of containment and the nature of the infectious agent(s)
being handled. Level of Risk to person or persons (Acceptable to Extreme) is determined by carrying
out a Microbiological RISK ASSESSMENT for the organism and the procedure. The Discipline of
Microbiology (DOM ) is working with Biological Risk Groups 1 and 2 Microorganisms and most
procedures warrant at either Acceptable to Low Risk ratings.
Accidental injection, cuts and abrasions
The affected individual should remove protective clothing, wash the hands and the affected part, apply
an appropriate skin disinfectant. If considered necessary, go to the Accident & Emergency
Department at UCHG and inform the person in charge about the cause of the wound and the organisms
involved. Complete accident report form and forward to the Safety office, NUI Galway (See SOP# 3 ;
Accidents, Spills, and Emergencies).
Accidental ingestion of Potentially Hazardous material
Protective clothing should be removed. If considered necessary, go to the Accident & Emergency
Department at UCHG and inform the person in charge about the cause of the wound and the organisms
involved. Complete accident report form and forward to the Safety office, NUI Galway . (See SOP# 3
; Accidents, Spills, and Emergencies).
Potentially Hazardous (Significant Risk) aerosol release (other than in a biological safety
cabinet)
All persons should immediately vacate the affected area and any exposed persons should be referred
for medical advice. The laboratory supervisor should be informed at once. No one should enter the
room for at least 1 h, to allow aerosols to be carried away and heavier particles to settle. If the
laboratory does not have a central air exhaust system, entrance should be delayed for 24 h. Signs
should be posted indicating that entry is forbidden. After the appropriate time, decontamination should
proceed. Appropriate protective clothing including respiratory protection should be worn for this.
Broken and spilled infectious substances, including cultures (Significant Risk)
Broken objects contaminated with infectious substances, including vials or containers, or spilled
infectious substances, including cultures, should be covered with a cloth or paper towels. The items
should be contained and labeled and disinfectant should then be poured over these and left for at least
30 min. The cloth or paper towels and the broken material may then be cleared away; glass fragments
should be handled with forceps. The contaminated area should then be swabbed with disinfectant. If
dustpans are used to clear away the broken material, they should be autoclaved or placed in an
effective disinfectant for 24 h. Cloths, paper towels and swabs used for cleaning up should be placed in
a contaminated-waste container. Gloves should be worn for all these procedures.
If laboratory forms or other printed or written matter are contaminated, the documents should be
handled with appropriate protection and should be copied onto another form and the original discarded
into the contaminated-waste container. The photocopying equipment should be disinfected.
Breakage of tubes containing Potentially Hazardous (Significant Risk) material in centrifuges
not having sealable buckets
If a breakage occurs or is suspected while the machine is running, the motor should be switched off
and the machine left closed for 30 min. If a breakage is discovered after the machine has stopped, the
lid should be replaced immediately and left closed for 30 min. Strong (e.g. thick rubber) gloves,
covered if necessary with suitable disposable gloves, should be worn for all subsequent operations.
Forceps, or cotton held in the forceps, should be used to retrieve glass debris. All broken tubes, glass
fragments, buckets, trunnions and the rotor should be placed in noncorrosive disinfectant known to be
active against the organisms concerned and left for 24 h and/or autoclaved. Unbroken, capped tubes
may be placed in disinfectant in a separate labeled container and recovered after 60 min. The
centrifuge bowl should be swabbed with the same disinfectant, at the appropriate dilution, left
overnight and then swabbed again, washed with water and dried. All materials used in the clean-up
should be treated as infectious waste.
Breakage of tubes inside sealable buckets (safety cups) (contained risk)
All sealed centrifuge buckets should be loaded and unloaded in a biological safety cabinet where the
specimens are not categorized for hazard and are of unknown Risk. If a breakage is suspected, the
bucket cap should be opened and left loose and the bucket autoclaved.
Fire, flood and natural disaster
Fire and other services should be involved in the development of emergency plans.
In the event of a flood or Fire, local emergency services should be warned of the potential hazards
within and/or near laboratory buildings. They should enter only when accompanied by a staff member.
Cultures and infectious materials should be collected in leakproof boxes or strong disposable bags and
stored in a safe location which present no additional hazard. . Salvage or final disposal should be
determined on the basis of local knowledge.
Vandalism
Action after vandalism is the same as that for other emergencies. Notification must be given to Safety
Office of any stolen Chemical/biological substances.
Emergency services: whom to contact
The telephone numbers and addresses of the following should be prominently displayed near all
laboratory telephones:
• the institution or laboratory itself (the address and location may not be known in detail by
the caller or the services called)
• director of the institution or laboratory
• laboratory supervisor
• biosafety officer
• fire services
• hospital/ambulance service (if a particular hospital has arranged to accept casualties, e.g.
high-riskpersonnel, the names of individual departments and doctors)
• police
• medical officer
• responsible technician
• water, gas and electricity services.
Emergency equipment
The following emergency equipment must be available:
•
•
first-aid kit
appropriate fire extinguishers, fire blankets
EMERGENCY PROCEDURE
NUIG EMERGENCY NUMBER
• Dial 9 for outside line then dial 999 or 112
OR
• 3333 / 2198 (University Security)
ON DISCOVERY OF FIRE OR SUSPICION OF FIRE
Raise the alarm by either:• Break glass unit which is located beside the fire stairs on the right.
• Telephoning the numbers above - stating extent and exact location of fire.
• Close all windows and doors where possible and safe to do so.
• Turn off electrical power and medical and other gases as appropriate.
• Do not take risks
• Do not endanger yourself.
IT IS BETTER TO RAISE ALARM PREMATURELY THAN TO WAIT
If the University emergency fire number is not answered you may alert the fire brigade by calling the
NUIG SWITCHBOARD OPERATOR (Dial 0).
ON HEARING THE FIRE ALARM
• Senior Staff Member (s) - proceed to scene of fire - most senior person to take
operations until arrival of Fire Brigade.
charge of
IF IN DOUBT – EVACUATE AND CARRY OUT ROLL CALL
•
Security and Porters - those assigned special duties proceed according to instructions remainder to Assembly Point.
•
All Other Staff - proceed to Assembly Point.
THE ASSEMBLY POINT (K) IS SITUATED
OPPOSITE MAIN ENVIRONMENTAL SCIENCE ENTRANCE, BESIDE
THE ÁRAS UÍ CATHAIL
GENERAL INSTRUCTIONS
It is the duty of every person in the Microbiology to behave such that as far as is reasonably
practicable nobody is exposed to danger from fire as a consequence of any act or omission of
that person. (See also Appendix 27c- Fire Prevention Measures (public areas).
DUTY OF ALL STAFF
• Exits are kept clear at all times.
• Keys are readily available for doors that may be locked.
• Ensure fire-fighting appliances are kept in position
• All areas are regularly checked daily for any potential fire hazards.
• To be familiar with fire orders, alarm and fire-fighting appliances and
attend the fire lectures and drills arranged.
HAZARD CONTROL : SAFE LABORATORY TECHNIQUES
Good Microbiological Techniques
Human error, poor laboratory techniques and misuse of equipment cause the majority of laboratory
accidents, injuries and work-related infections. These guidelines provide a summary of technical
methods that are designed to avoid or minimize the most commonly reported problems of this nature.
Safe handling of specimens in the laboratory
Improper collection, internal transport and receipt of specimens in the laboratory carry a risk of
infection to the personnel involved.
Specimen containers
Specimen containers may be of glass or preferably plastic. They should be robust and should not leak
when the cap or stopper is correctly applied. No material should remain on the outside of the container.
Containers should be correctly labelled to facilitate identification. Specimen request or specification
forms should not be wrapped around the containers but placed in separate, preferably waterproof
envelopes.
Transport of specimens within the facility
To avoid accidental leakage or spillage, secondary containers, such as boxes, should be used, fitted
with racks so that the specimen containers remain upright. The secondary containers may be of metal
or plastic, should be autoclavable or resistant to the action of chemical disinfectants, and the seal
should preferably have a gasket. They should be regularly decontaminated.
Receipt of specimens
Laboratories that receive large numbers of specimens should designate a particular room or area for
this purpose.
Opening packages
Personnel who receive and unpack specimens should be aware of the potential health hazards
involved, and should be trained to adopt universal precautions, particularly when dealing with broken
or leaking containers. Specimens of unknown or significant Risk should be unpacked in a biological
safety cabinet. Disinfectants should be available.
Use of pipettes and pipetting aids.
• A pipetting aid should always be used. Pipetting by mouth must be prohibited.
• Always perform aerosol generating pipetting operations in a Biosafety Cabinet or hood.
• All pipettes should have cotton plugs to reduce contamination of pipetting devices and
blocks the escape of aerosolised material.
• Air should never be blown through a liquid containing infectious agents. This generates aerosol.
• Do not mix biohazardous fluids by repeated suction and expulsion from pipettes. This generates
aerosol
• Infectious materials should not be mixed by alternate suction and expulsion through a pipette.
•
•
Avoid dispersion of infectious material accidentally dropped from a pipette, a
disinfectant-soaked cloth or absorbent paper should be placed on the working surface;
this should be autoclaved or discarded as infectious waste after use.
Contaminated pipettes should be completely submerged in a suitable disinfectant contained in an
unbreakable container. They should be left in the disinfectant for 18-24 h
before disposal.
• Syringes fitted with hypodermic needles must not be used for pipetting. Blunt cannulas should be
used instead of needles. There are devices for opening septum-capped bottles that allow pipettes to
be used and avoid the use of hypodermic needles and syringes.
Avoiding the dispersal of infectious materials
• The risk of spatter of infectious material in an open Bunsen burner flame should be avoided by
using an enclosed electric microincinerator to sterilize transfer loops. Disposable transfer loops,
which do not need to be resterilized, are preferable.
•
Discarded specimens and cultures (not glassware) for autoclaving and/or disposal should be placed
in leakproof containers, e.g. laboratory discard bags.
•
Working areas must be decontaminated with a suitable disinfectant at the end of each work period.
Use of biological safety cabinets
(See also SOP # 14:Operating Procedure for Biological Safety Cabinet)
• The use and limitations of biological safety cabinets should be explained to all potential users, with
reference to national standards and relevant literature. Written protocols or safety or operations
manuals should be issued to staff. In particular, it must be made clear that the cabinet will not
protect the operator from spillage, breakage or poor technique.
•
The cabinet must not be used unless it is working properly.
•
The glass viewing panel must not be fully opened when the cabinet is in use.
•
Apparatus and materials in the cabinet must be kept to a minimum. Air circulation at the rear
plenum must not be blocked. Materials should be surface-decontaminated before placing them
inside the working area of the cabinet.
•
Bunsen burners must not be used in the cabinet. The heat produced will distort the air flow and
may damage the filters. An electric microincinerator is permissible but sterile disposable transfer
loops are better.
•
All work must be carried out in the middle or rear part of the working surface and be visible
through the viewing panel.
•
Traffic behind the operator should be minimized.
•
The operator should not disturb the air flow by repeated removal and reintroduction of his or her
arms.
•
The front air grill must not be blocked with notes, pipettes or other materials, as this will disrupt
the air flow causing potential contamination of the material and exposure of the operator.
•
The surface of the biological safety cabinet should be wiped using an appropriate disinfectant after
work is completed and at the end of the day.
•
The cabinet fan should be run for at least 5 min before beginning work and after completion of
work in the cabinet.
•
Place material such that clean and contaminated items do not meet.
•
Place contaminated material container at right rear
Avoiding ingestion of infectious materials and contact with skin and eyes
• Large particles and droplets released during microbiological manipulations settle rapidly on bench
surfaces and on the hands of the operator. Disposable gloves should be worn. Laboratory workers
should avoid touching their mouth, eyes and face.
•
Food and drink must not be consumed or stored in the laboratory.
•
There should be no gum-chewing in the laboratory.
•
Cosmetics should not be applied in the laboratory.
•
The face, eyes and mouth should be shielded or otherwise protected during any operation that may
result in the splashing of potentially infectious materials.
Avoiding injection of infectious materials
• Needles should never be recapped
• Plastic Pasteur pipettes should replace those made of glass.
Separation of serum
• Only properly trained staff should be employed for this work.
• Gloves and eye and mucous membrane protection should be worn.
• Splashes and aerosols can only be avoided or minimized by good laboratory technique. Blood and
serum should be pipetted carefully, not poured. Pipetting by mouth must be forbidden.
• After use, pipettes should be completely submerged in a suitable disinfectant. They should remain
in the disinfectant for at least 18 h before disposal, or washing and sterilization for reuse.
• Discarded specimen tubes containing blood clots, etc. (with caps replaced) should be placed in
suitable leakproof containers for autoclaving and/or incineration.
• A solution of disinfectant (70% IMS or 1% Vircon), freshly prepared daily, should be available for
clean-up of splashes and spillages.
Use of centrifuges
• Centrifuges should be operated according to the manufacturer’s instructions.
• Centrifuges should be placed at such a level that workers of less than average height can see into
the bowl to place trunnions and buckets correctly.
• Centrifuge tubes and specimen containers for use in the centrifuge should be made of thick-walled
glass or preferably of plastic (autoclaveable) and should be inspected for defects before use.
• Tubes and specimen containers should always be securely capped (screw-capped if possible) for
centrifugation.
• For Samples of unknown or Significant Biohazard Risk, the buckets must be loaded, equilibrated,
sealed and opened in a biological safety cabinet. Buckets and trunnions should be paired by weight
and, with tubes in place, correctly balanced.
• The amount of space that should be left between the level of the fluid and the rim of the centrifuge
tube should be given in manufacturer’s instructions.
• Distilled water or alcohol (70%) should be used for balancing empty buckets.
• When using angle head centrifuge rotors, care must be taken to ensure that the tube is not
overloaded as it might leak.
• The interior of the centrifuge bowl should be inspected daily for staining or soiling at the level of
the rotor. If staining or soiling are evident then the centrifugation protocols should be re-evaluated.
• Centrifuge rotors and buckets should be inspected daily for signs of corrosion and for hair-line
cracks.
• Buckets, rotors and centrifuge bowls should be decontaminated after each use.
• After use, buckets should be stored in an inverted position to drain.
Use of homogenizers, shakers, blenders and sonicators
• Domestic (kitchen) homogenizers are not recommended for laboratories as they may leak or
release aerosols. Laboratory blenders and stomachers are safer.
• Pressure builds up in the vessel during the operation of homogenizers, shakers and sonicators.
Aerosols containing infectious materials may escape from between the cap and the vessel. Plastic,
in particular, polytetrafluoroethylene (PTFE) vessels are recommended because glass may break,
releasing infectious material and possibly wounding the operator.
• When in use, homogenizers, shakers and sonicators should be covered by a strong transparent
plastic casing. This should be disinfected after use. Where possible, these machines should be
operated, under their plastic covers, in a biological safety cabinet.
• At the end of the operation the containers should be opened in a biological safety cabinet.
• Hearing protection is provided for people using sonicators.
Use of tissue grinders
• Glass grinders should be held in a wad of absorbent material in a gloved hand. Plastic (PTFE)
grinders are safer.
• Tissue grinders should be operated and opened in a biological safety cabinet.
Care and use of refrigerators and freezers
• Refrigerators, deep-freezers and solid carbon dioxide (dry-ice) chests should be defrosted and
cleaned periodically, and any ampoules, tubes, etc. that have broken during storage removed. Face
protection and heavy duty rubber gloves should be worn during cleaning. After cleaning, the inner
surfaces of the cabinet should be disinfected.
•
•
•
•
All containers stored in refrigerators, etc. should be clearly labelled with the scientific name of the
contents, the date stored and the name of the individual who stored them. Unlabelled and obsolete
materials should be autoclaved and discarded.
An inventory must be maintained of the freezer’s contents.
Flammable solutions must not be stored in a refrigerator unless it is explosion-proof. Notices to
this effect should be placed on refrigerator doors.
Where appropriate Biohazard Warning Signs should be placed on refrigerator doors.
Opening of ampoules containing lyophilized infectious materials
Care should be taken when ampoules of freeze-dried materials are opened, as the contents may be
under reduced pressure and the sudden inrush of air may disperse some of the materials into the
atmosphere. Ampoules should always be opened in a biological safety cabinet. The following
procedures are recommended for opening ampoules.
•
•
•
•
•
•
First decontaminate the outer surface of the ampoule.
Make a file mark on the tube near to the middle of the cotton or cellulose plug, if present
Hold the ampoule in a wad of alcohol-soaked cotton to protect hands before breaking it at a file
scratch.
Remove the top gently and treat as contaminated material.
If the plug is still above the contents of the ampoule, remove it with sterile forceps.
Add liquid for resuspension slowly to the ampoule to avoid frothing.
Storage of ampoules containing infectious materials
Ampoules containing infectious materials should never be immersed in liquid nitrogen because
cracked or imperfectly sealed ampoules may break or explode on removal. If very low temperatures
are required, ampoules should be stored only in the gaseous phase above the liquid nitrogen.
Otherwise, infectious materials should be stored in mechanical deep-freeze cabinets or on dry ice.
Laboratory workers should wear eye and hand protection when removing ampoules from cold
storage.The outer surfaces of ampoules stored in these ways should be disinfected when the ampoules
are removed from storage.
SPECIAL PRECAUTIONS WITH BLOOD AND OTHER BODY FLUIDS,
TISSUES AND EXCRETA
The precautions outlined below are designed to protect laboratory workers against infection by blood
borne pathogens.
Collection, labeling and transport of specimens
• Universal precautions should always be followed; gloves should be worn for all procedures.
• The tubes should be placed in adequate containers for transport to the and within the laboratory
facility. Request forms should be placed in separate water-proof bags or envelopes.
Containment
• Diagnostic work may be done in a basic laboratory - Biosafety Level 2, preferably one dedicated
for this purpose.
Opening specimen tubes and sampling contents
• Specimen tubes should be opened in a Class I or Class II biological safety cabinet.
• Gloves must be worn. Eye and mucous membrane protection is also recommended (goggles or
shield (visor)).
• Protective clothing should be supplemented with a plastic apron.
• The stopper should be grasped through a piece of paper or gauze to prevent splashing.
Glass and “sharps”
• Plastics should replace glass wherever possible. Only laboratory grade (borosilicate) glass should
be used, and any article that is chipped or cracked should be discarded.
• Hypodermic needles must not be used as pipettes. Blunt cannulas are permitted.
Films and smears for microscopy
Fixing and staining of blood, sputum and faecal samples for microscopy does not necessarily kill all
organisms or viruses on the smears. These items should be handled with forceps, stored appropriately,
and decontaminated and/or autoclaved before disposal.
Automated equipment (sonicators, vortex mixers)
• Equipment should be of the closed type to avoid dispersion of droplets and aerosols.
• Effluents should be collected in closed vessels for further autoclaving and/or disposal.
• Equipment should be disinfected at the end of each session, following the manufacturer’s
instructions.
Tissues
• Formalin fixatives should be used. Small specimens, e.g. from needle biopsies, can be fixed and
decontaminated within a few hours, but larger specimens may take several days.
• Frozen sectioning should be avoided. Should it be essential, the cryostat should be shielded and the
operator should wear a safety shield (visor). For decontamination, the temperature of the
instrument should be raised to 20 °C.
Decontamination
Hypochlorites and high-level disinfectants are recommended for decontamination. Freshly prepared
hypochlorite solutions should contain available chlorine at 1 g/l for general use and 10 g/l for blood
spillages.
HAZARD CONTROL - WASTE DISPOSAL
(See Relevant Sections:
Appendix 23c - Approved Handling Procedure - Sharps Material
Appendix 23d - Approved Handling Procedure - Biohazard Material).
The disposal of laboratory and medical waste is subject to various regional, national and international
regulations and the latest versions of such relevant documents must be consulted before designing and
implementing a programme for handling, transportation and disposal of biohazardous waste
BIOLOGICAL WASTE
Autoclaved waste may be disposed of by off-site incineration.
(See SOP# 2 BIOLOGICAL WASTE DISPOSAL).
All GMO Biological Waste is autoclaved and brought to a central collection point from where it is
collected weekly by “ECOSAFE Technologies Limited” and brought to Dublin. The waste is
accompanied by a C1 Form (Waste Management Regulations). Treatment involves shredding and
steaming to sterilise. It is then placed in FIBIC Bags and sent to landfill for burial.
CHEMICAL WASTE
Hazardous Chemical Waste is disposed of through the University Health and Safety Office (HSO). A
list of chemicals for disposal are send to the HSO who arranges for their disposal through the
University contractor.
BIOHAZARDOUS SHARPS WASTE (See SOP# 2 :BIOLOGICALWASTE DISPOSAL).
All glass slides or glass from breakages are placed in sharps bins, they then follow the same route as
the biological waste.
Non biological glass breakages are placed in the larger 'Glass & Aerosol Waste Boxes' and disposed
through the University contractor.
Appendix 23c - Approved Handling Procedure - Sharps Material Page 1 of 1 .
“Sharps” refers to any equipment capable of causing a puncture or incision wound. Such equipment may be
reusable e.g. knives, scissors or disposable e.g. scalpel blades, hypodermic needles. The term sharps also
includes broken equipment or materials, which as a consequence of their damage can cause similar injuries.
Once used, all such sharps must be handled properly to reduce the risk of further injury.
1.
Sharps must never be left lying about. Once used, sharps must be immediately either
(1). disinfected, cleaned and/or sterilised as appropriate and put away safely into the allocated
storage place OR
(2). disposed of safely.
2.
Needles, once used should not be resheathed, but immediately disposed of into an approved
sharps container. Broken items of equipment which have sharp ends or edges must be placed in a
container that provides protection for those who may handle it.
3. “Sharps” containers must be constructed from puncture resistant material. They should be
impervious to liquids and be provided with a closure device that will remain sealed during transit from
the laboratory for destruction. Containers which do not comply with these specifications must not be
used. (Relevant BS standards BS4851(1982) and BS 5213(1975) BS7320 (1990). Externally the
container should be clearly marked as to the hazards “Danger”
“Contaminated sharps only”.
Sharps containers should not be allowed to fill more than 80% capacity.
In each laboratory, a person(s) must be identified who will be responsible for removing and providing
sharps containers at suitable intervals. Wear appropriate gloves when handling sharps containers.
4.
Before handling sharps, verify that there is a first aid kit in the vicinity and know who are the
local First Aiders to be contacted in an emergency.
In the event of a sharps injury:
•
•
•
encourage bleeding and wash under running water;
seek medical advice immediately;
accident to be reported and recorded as soon as possible, and investigated to determine
cause.
5.
Once filled to the 80% level, all containers for sharps or other biohazardous waste must be
sealed by laboratory staff, in tear resistant bags (minimum gauge 400), and suitably labelled (Appendix
23e) and colour coded.
Black: Normal Household waste. Not to store or transport biohazardous waste.
Yellow: All waste destined for destruction.
Light blue or transparent with light blue inscriptions:
Waste for autoclaving (or equivalent treatment) before ultimate disposal.(BS 381C:1980)
6. Such bags must be securely stored until collected for incineration.
Non-Laboratory areas:
In non-laboratory areas where University personnel may encounter sharps e.g. broken glass,
appropriate arrangements for their safe handling and disposal must be made. For example, access to
glass-aerosol boxes and handling in accordance with these procedures.
Appendix 23d - Approved Handling Procedure - Biohazard Material Page 1 of 1 .
Biological waste includes all waste which because of its biological or physical nature presents a risk of
infection or inoculation injury, and therefore requires specific disposal procedures. All biohazardous waste
which is likely to be infected must be sterilised before leaving the laboratory or research area.
1. Designation of biohazard waste.
• sharps*;
• contaminated tissues, etc.;
• human blood, plasma and other clinical waste;
• inoculated microbial media plates;
• contaminated Personal Protective Equipment e.g. disposable gloves/other equipment
• soils; - cell culture/tissue cultures
2. Segregation.
Biohazard waste will be segregated as follows:
(a) Sharps containers - All sharps;
• Contaminated glassware.
(b) Biohazard bags - Contaminated tissues;
• Human tissue, blood & other fluids, etc.;
• Contaminated PPE, etc.;
• Microbial specimens.
• Soils
(c) Glass/aerosol boxes - non-contaminated glass and plastic-ware.
3. Packing/labelling.
All biohazard waste will be disposed of directly into the designated container. These containers will
be labelled as to their biohazard, and any other hazard at the place of origin, using the following colour
coding symbols (BS 381C:1980):
Black:
Normal Household waste. Not to store or transport biohazardous waste.
Yellow:
All biohazardous waste destined for destruction.
Light blue or transparent with light blue inscriptions:
Waste for autoclaving (or equivalent treatment) before ultimate disposal.
All such waste must be autoclaved prior to disposal.
No glass or other sharps material are allowed in bags.
Do not overfill containers. In the particular cases of bags and sharps containers, they should not be
filled to more than 80% capacity.
4. Storage
Biohazard waste must be stored in a secure, designated area, which is vermin-proof and cleansable.
All biohazardous waste which is biodegradeable must be frozen ASAP after packaging to prevent
further contamination and facilitate collection.
5. Tracking
All biohazard waste will be labelled (Appendix 23e) as to the laboratory of origin, dates on which it
was produced and signature of laboratory person responsible for supervising the safe disposal of such
waste.
6. Transport and disposal
This will be provided by the clinical waste handling service engaged by NUI, Galway.
• “Sharps” refers to any equipment capable of causing a puncture or incision wound.
SOP #1.
Safe Operating Procedure
AVOIDING THE PRODUCTION OF BIOLOGICAL AEROSOLS
Page 1 of 2
Many documented laboratory-acquired infections resulted from production and inhalation of infectious
aerosols. Aerosol production can occur when using certain laboratory equipment, if adequate
precautions are not taken.
This SOP provides guidance for avoiding the production of bioaerosols.
Centrifuge Operations
If a microorganism isn’t contained within a centrifuge, aerosols can be produced during the high spin
process. This could occur with uncapped samples, or when a leak, spill, or breakage of the tube occurs.
Minimize the risk of aerosol production when centrifuging by observing the following precautions:
• Use unbreakable tubes.
• Avoid overfilling the tubes.
• Use centrifuge tubes with screw caps.
• Use outer, sealable safety cups that can be loaded and unloaded in a biological
safety cabinet. Decontaminate with appropriate disinfectant the inside and outside of the cups or
buckets before and after use and inspect seals regularly for deterioration.
• Ensure that the centrifuge is properly balanced.
• Do not open the lid during or immediately after operation.
• Allow the centrifuge to come to a complete stop and wait at least five minutes before opening.
This allows time for aerosols to settle if leakage or breakage occurred during the centrifugation run.
• Never exceed the specified limitations of the rotor as listed in the owner’s manual.
When possible, install the centrifuge in an enclosed, specially ventilated area that discharges air from
the space through a HEPA filter.
Mixing Operations
Sonicators, shakers, and homogenizers can generate aerosols during operation.
Minimize the risk of aerosol production when mixing by observing the following
precautions:
• Operate mixing equipment in a biological safety cabinet.
• Use sealed vessels during mixing and allowing the vessels to settle before opening.
• Open mixing vessels inside of a biological safety cabinet.
• Check the condition of the mixing equipment routinely for deterioration.
• Disinfect all surfaces of mixing devices before and after use.
Vacuum and Aspirating Equipment
• Minimize the risk of generating aerosols during vacuum and aspiration operations by
observing the following precautions:
• Use non-breakable equipment (i.e., not glass).
• Ensure that vacuum equipment is fitted with a HEPA filter.
• Place appropriate disinfectant in the overflow flask of the aspirating equipment.
SOP# 1.
Safe Operating Procedure
AVOIDING THE PRODUCTION OF BIOLOGICAL AEROSOLS
Page 2 of 2
Needles and Syringes
Minimize the risk of aerosol production while using needles and syringes by observing
the following precautions:
• Perform all operations with needles and syringes in a biological safety cabinet.
• Fill syringes carefully. Avoid frothing or introducing air bubbles.
• Discharge air from the syringe before inserting it in to a rubber stopper on bottle to prevent
pressurising bottle.
• Wrap the needle and stopper in a cotton ball or pad moistened with an appropriate disinfectant
when removing the needle from the rubber-stoppered bottle.
Pipettes
Minimize the risk of aerosol production while using pipettes by observing the following
precautions:
• Use cotton-plugged pipettes.
• Gently expel the contents of the pipette close to the surface of the liquid or allow
it to flow down the side of the container.
• Do not mix the contents of a container by alternating suction and blowing with a pipette.
• Use TD pipettes instead of TC pipettes. The last drops from a TD pipette do not
need to be expelled or blown-out to get an accurate measurement.
• Submerge used non-disposable pipettes horizontally in a disinfectant solution. Dropping them in
vertically may force out any remaining liquid.
Transfer Loops
Minimize the risk of aerosol production while using transfer loops by observing the
following precautions:
• When disinfecting the loop between inoculations, hold the loop close to, but not
in, the flame until the material has charred, then fully insert it into the flame.
• Where possible, substitute an enclosed micro-incinerator for an open flame burner.
• Use disposable inoculating tools where possible
The information provided in this SOP supplements the NUIG Safety Statement.
Please refer to the full program document for more information.
SOP# 2
BIOLOGICAL WASTE DISPOSAL
Page 1 of 2
1. SCOPE
This SOP describes the methods to be used to disinfect and dispose of laboratory-generated
infectious wastes.
No GMO wastes are to leave the premises or control of the Principal Investigator(s) without
first being sterilized to ensure that they present no harm to others or the environment.
Non-GMO solid waste materials may be disposed of directly by using the designated ECOSAFE
Waste bins.
2. PROCEDURE
SOLID WASTE
Solid wastes, such as pipettes and culture plates, may be stored in the biohazards area following
disinfection or while being disinfected by immersion in suitable disinfectant. Other wastes, such
as lab coats, gloves, and other articles of PPE without gross contamination, can be stored in the
biohazards area in appropriately labeled containers (polyethylene biohazard bags, at least 3 mils
thick). All solid wastes, suitable for autoclaving (121 degrees C, 30 minutes), should be treated
this way prior to removal from the premises or control of the Principal Investigator(s). Disposal is
then accomplished by placing the autoclaved waste in the designated ECOSAFE Waste bins area
for pickup.
LIQUID WASTE
Liquid wastes should be carefully poured into the appropriate disinfectant to inactivate the
biohazardous agent. Following sufficient contact time, the disinfected biohazardous liquid maybe
poured directly down the drain. This should be done carefully to avoid splashing and aerosol
generation. Afterwards the drain should be flushed with disinfectant of sufficient quantity to at
least fill the trap. Large volumes of liquid wastes should be autoclaved prior to disposal down the
drain.
SHARPS
Sharps, such as hypodermic needles and scalpel blades, are required by to be segregated from
other wastes. Discard all sharps in labeled, puncture-resistant "sharps" approved containers.
1. Sharps bins should be located in strategic safe locations near the user.
2. All needles, syringes and sharps must be disposed of immediately into the sharps bin provided.
3. Sharps bins must be available in sufficient numbers.
SOP# 2
BIOLOGICAL WASTE DISPOSAL
Page 2 of 2
4. Sharps should be dropped into bin. They should never be pushed down into bin.
5. Sharps bins should be filled to the mark indicated in the container, i.e. three quarters full.
They must not be overfilled.
6. Fingers and hands must never be allowed past the lever of the lid.
7. Needles and syringes should be disposed of as a single unit. Never re-sheath a needle.
8. Sharps bins should be kept out of the reach of unauthorised persons.
9. The lid should be safely secured in the locked position prior to disposal.
10. Sharps bins should be stored in a safe location prior to collection for disposal by a
designated responsible person.
SOP #3. :
Accidents, Spills, and Emergencies
Page 1 of 3 .
Accident Reporting
•
All accidents must be reported to the laboratory supervisor and the principal investigator within
24 hours (see Table below).
•
The principal investigator, researcher, lab director or supervisor should maintain a copy of all
types of reports.
Summary Table of accident reporting requirements
If the accident involves...
Complete a...
And send a
Comments
copy to...
Injuries on university-owned or
Accident Report HSO
A copy of this report must
occupied space not requiring immediate Form
be kept in the Chief
medical attention
Technician's personnel file
should medical treatment
be required in the future
Injuries on university related fieldwork Accident Report HSO
A copy of this report must
Form
be kept in the Chief
Technician's personnel file
should medical treatment
be required in the future
Potential exposure to bloodborne
Accident Report HSO
A copy of this report must
pathogens
Form
be kept in the Chief
Technician's personnel
file.
Exposure to other biological agents,
radiation, or chemicals
Accident Report Radiation
Form
Officer
and
HSO
A copy of this report must
be kept in the Chief
Technician's personnel
file.
SOP #3.
Accidents, Spills, and Emergencies
Page 2 of 3 .
Spills
•
Every work area is subject to accidents. The resulting damage depends upon how quickly and
completely the spills are cleaned up and removed.
•
A means for prompt spill control should be provided for all work areas using hazardous
materials.
•
Each lab must prepare a spill protocol SOP that must be included in this manual. It is the
responsibility of the principal investigator, researcher, lab director or supervisor to prepare labspecific spill protocols.
•
Spill kits should be located in an easily identifiable location central to laboratory operations.
•
Each employee must be trained to handle small-scale spills.
•
An inventory checklist should be kept with each spill kit and regularly matched against the
contents of the kit. A list of suggested inventory items for each type of spill are as follows .
Spill Kits
Basic Biological Spill Kit (in Upper and Lower Hallways)
•
Disinfectant (appropriate for specific agents(s) in use e.g., 70% alcohol or 1% Virkon / neat
virkon). (See HAZARD CONTROL DISINFECTANTS AND STERILIZATION).
•
Absorbent material (e.g., paper towels, sponges).
•
Waste Container (e.g., biohazard bags, sharps container).
•
Personal Protective Equipment (e.g., gloves, eye and face protection).
•
Autoclavable Tools (e.g., forceps, dustpan, broom).
Basic Chemical Spill Kit (in Upper and Lower Hallways)
•
Cleaner (e.g., soap)
•
Absorbent Material (e.g., paper towels, vermiculite/kitty litter)
•
Waste Container (e.g., plastic bags and duct tape)
•
Personal Protective Equipment (e.g., coveralls, chemically resistant gloves and boots/booties,
safety goggles and respirator (available from Chief Technician)).
•
Tools (e.g., broom and dustpan, plastic bucket, mop, plastic scoops)
Basic Radiological Spill Kit (Room 204 : Radiotracer Suite)
•
Decontamination Solution (e.g., soap)
•
Absorbent Material (e.g., paper towels)
•
Radioactive Waste Container(s)
•
Personal Protective Equipment (i.e., disposable gloves, safety goggles/glasses)
•
Tools (e.g., tongs, radiation survey meter, wipes)
•
Perspex shield
SOP #3.
Accidents, Spills, and Emergencies
Page 3 of 3 .
Emergencies
Information provided in this section is not intended to take the place of a complete course of
instruction under the direction of a qualified instructor. Instruction in emergency response and first aid
is highly recommended for individuals who may have to perform such duties.
Each lab must develop emergency procedures for plausible events that may occur in a biological
use lab (e.g., heart attack victim, fire in lab).
Basic Emergency Principles
If in doubt - call for assistance
Where possible do the following
•
Never rush into an emergency situation; protect yourself first from danger
•
Survey the scene
•
Survey the victim(s) and remove them from scene if possible.
•
Obtain help : Dial 999 or 112 yourself or give that responsibility to bystanders. If possible, send
two people to make the call to ensure it is made accurately. Ask the caller(s) to report back and tell
you what the dispatcher said. The 911 dispatcher will require the following information:
•
o
The exact location of the emergency (address, city or town, nearby intersections,
landmarks, name of the building, floor, apartment or room number)
o
The telephone number of the phone being used
o
The caller's name
o
What happened
o
The number of victims
o
The victims' condition
o
The help being given
o
Name of any hazardous material released or on the victims
Do a secondary survey of the scene . Recheck the scene to determine if any changes have
occurred. Reassure the casualty and monitor vital signs.
SOP #4a.
•
•
•
Minor Biological Spill inside Biological Safety Cabinet (BSC).
Have a complete biological spill kit ready to go before you start the clean-up
Initiate clean-up as soon as possible
Allow cabinet to operate during clean-up.
Personal Protective Equipment:
Labcoat, safety goggles, gloves.
Equipment:
Disinfectant, biohazard bags, paper towels and/or cloths.
Procedure:
1. Don personal protective equipment.
2. Apply disinfectant to spill and allow a minimum of 20 minutes contact time.
3. Soak up spilled material with paper towels.
4. Wipe down all reachable cabinet surfaces with disinfectant.
5. If cabinet has a catch basin, flood this with disinfectant.
6. Place disposable contaminated materials into red biohazard bag and autoclave before
discarding as regulated medical waste.
7. Place contaminated reusable items in biohazard bags, or heat resistant pans or
containers with lids before autoclaving and further clean-up.
8. Expose non-autoclavable materials to disinfectant, 20 minutes contact time, before
removal from the BSC.
9. Remove protective clothing used during clean-up and place in biohazard bag for
autoclaving.
10. The cabinet should be run 10 minutes after clean-up before resuming work or turning
off the cabinet.
11. Inform all users of the BSC as well as the laboratory supervisor/principal investigator
about the spill and successful clean-up as soon as possible.
Page 1 of 1
SOP #4b.
•
•
•
Minor (Acceptable / Low Risk) Biological Spill outside Biological Safety Cabinet
(BSC).
Page 1 of 1
Clear area of all personnel
Have a complete biological spill kit ready to go before you start the clean-up
Initiate clean-up as soon as possible
Personal Protective Equipment:
Labcoat, safety goggles, gloves.
Equipment:
Disinfectant, biohazard bags, paper towels and/or cloths.
Procedure:
1. Don personal protective equipment.
2. Apply disinfectant to spill and allow a minimum of 20 minutes contact time.
3. Soak up spilled material with paper towels.
4. Wipe down surrounding surfaces with disinfectant.
5. Place disposable contaminated materials into biohazard bag and autoclave before
discarding as regulated biological waste.
6. Place contaminated reusable items in biohazard bags, or heat resistant pans or
containers with lids before autoclaving and further clean-up.
7. Expose non-autoclavable materials to disinfectant, 20 minutes contact time, before
removal.
8 .Remove protective clothing used during clean-up and place in biohazard bag for
autoclaving.
SOP #5
Extensive Biological Spill (Significant Risk ) inside Lab and Outside Biological
Safety Cabinet (BSC).
Page 1 of 1
• Clear area of all personnel
• Wait 30 minutes for aerosol to settle before entering spill area
• Initiate clean-up as soon as possible
• Have a complete biological spill kit ready to go before you start the clean-up
• Personal Protective Equipment:
BSL2 agents – Disposable long-sleeved gown, booties, safety glasses, mask, and gloves
Equipment:
Disinfectant, biohazard bags, paper towels and/or cloths, autoclavable plastic dustpan and squeegee,
cart with side rails, stainless steel bucket/pan
Procedure:
1. Remove contaminated clothing and place in biohazard bag to be autoclaved.
2. Don disposable personal protective equipment.
3. Soak paper towels in disinfectant and place over spill.
4. Encircle spill with additional disinfectant being careful to minimize aerosolization.
5. Allow 20 minutes contact time to ensure effectiveness of disinfectant.
6. Decontaminate all items within spill area.
7. Remove broken glassware with forceps or broom and dustpan and place in sharps container.
8. Wipe equipment with 1:10 bleach to water solution, then with 70% alcohol.
9. Place large equipment in separate bags and on the cart for transport to autoclave.
10. Place disposable contaminated materials into red biohazard bag and autoclave before
discarding as regulated medical waste.
11. Place contaminated reusable items in biohazard bags, or heat resistant pans or
containers with lids before autoclaving and further clean-up.
12. Expose non-autoclavable materials to disinfectant, 20 minutes contact time, before
removal from the BSC.
13. Inform all users of the BSC as well as the laboratory supervisor/principal investigator
about the spill and successful clean-up as soon as possible.
Note: If spill occurs in a non-containment laboratory, clean-up should begin as soon as
personnel leave the area.
SOP #6
Biological Spill outside Lab, During Transportation
Page 1 of 1
• Clear area of all personnel
• Initiate clean-up as soon as possible
Prevent this from occurring by transporting all biohazardous material in an unbreakable well-sealed
primary container placed inside a second unbreakable lidded container labeled with the biohazard
symbol (e.g., cooler, plastic pail)
Personal Protective Equipment:
BSL2 agents – Disposable long-sleeved gown, booties, safety glasses, mask, and gloves
Equipment:
Disinfectant, biohazard bags, paper towels and/or cloths, autoclavable plastic dustpan and squeegee,
stainless steel bucket/pan
Procedure:
1. Remove contaminated clothing and place in biohazard bag to be autoclaved.
2. Don disposable personal protective equipment.
3. Soak paper towels in disinfectant and place over spill.
4. Encircle spill with additional disinfectant being careful to minimize aerosolization.
5. Allow 20 minutes contact time to ensure effectiveness of disinfectant.
6. Decontaminate all items within spill area.
7. Remove broken glassware with forceps or broom and dustpan and place in sharps container.
8. Wipe equipment with appropriate disinfectant.
9. Place large equipment in separate bags and on the cart for transport to autoclave.
10. Place disposable contaminated materials into biohazard bag and autoclave before
discarding as regulated biological waste.
11. Place contaminated reusable items in biohazard bags, or heat resistant pans or
containers with lids before autoclaving and further clean-up.
SOP #12
ACCIDENT TREATMENT AND REPORTING
Page 1 of 1
1. SCOPE
It is important that employees understand the actions to be taken following an occupational
exposure so that the appropriate medical treatment, prophylaxis and/or post-exposure
evaluations are utilized to prevent infection. An exposure incident is the specific eye, mouth or
other mucous membrane, non-intact skin, parenteral contact with blood or other potentially
infectious agents that results from the performance of an employee's duties. An example would
be a puncture wound with a contaminated needle.
2. PROCEDURE
a. Provide immediate first-aid. Stop the bleeding of wounds and wash the affected
areas with disinfectant/soap.
b. Report the incident to your supervisor. The supervisor records the details of the
exposure incident including the route of exposure, the infectous agent and an
estimate of the dosage.
c.
Report for treatment at:
During normal working hours (8 A.M. - 5 P.M. Mon-Fri)
Student health Unit (ext. 2604)
After 5 P.M. or weekends
University College Hospital Galway
Emergency Department
911 or 4-2121
Employee and Supervisor fill out Employer's Report of Occupational Injury Form and file
within 48 hours HSO.
SOP #13
Working with Human-Derived Material
with Specific Reference to the Risk of Needle Stick Injuries.
Page 1 of 4
1. Objective
These procedures set out the recommended measures to be adopted and implemented by NUI, Galway
laboratories working with human-derived material1. The objective of these suggested procedures is to
ensure that:
• Laboratories have adequate procedures and practical measures in place to ensure the reduction
of the risk of transmission of human pathogens to other personnel.
• There are adequate emergency procedures in place where there is a risk of accidental exposure
e.g. in the event of a needle stick injury.
2. Prevention Procedure
All human-derived material must ALWAYS be handled as potentially infectious material, even if the
tissue has been found to be negative for HIV, hepatitis B (HBV) or hepatitis C (HCV) or any other
potential pathogen. In all cases UNIVERSAL PRECAUTIONS must always be applied. Attention is
drawn to the University’s Approved Handling Procedures for Sharps Material and Biohazard Material.
These are found in appendices 23 (c) and 23 (d) of the University Safety Statement, Part 7,
Appendices.
2.1 Risk Assessment Procedure
Persons responsible for the safety of staff, students and visitors who may be exposed to human-derived
material being stored, used or otherwise handled in their laboratories and work areas for which they
are responsible, have a legal obligation to the assess the risks of exposure to such material and to have
measures and controls in place to eliminate such risks or to reduce them to an acceptable working
level. The procedure for achieving this is as follows:
i. Identify the task in which potentially infectious material is handled.
ii. Identify the source of the specimen.
iii. Identify the nature of the specimen.
iv. Assess the treatments to which the specimen has been subjected.
v. Identify the possible biological agents in the specimen.
vi. Identify and implement measures to eliminate/prevent exposure as far as possible (see 2.3 below).
vii. Assess the remaining risks in terms of severity and likelihood.
viii. Identify and ensure that all the required control measures are being implemented.
ix. Review regularly.
Infected persons may transmit infection to their families and other contacts, and hence pose a risk to
the community. Account must also be taken of risks to people who may be more severely affected due
to pregnancy, compromised immunity, pre-existing disease, or the effects of medication.
SOP #13
Working with Human-Derived Material
with Specific Reference to the Risk of Needle Stick Injuries.
Page 2 of 4
2.2 Control Procedures
Mechanisms for controlling the hazards posed by human-derived material are approached in a
prioritised manner. Elimination is the primary mechanism of control, followed by Substitution,
Engineering, Administrative, Personal Hygiene, and finally, and lastly, Personal Protective Equipment.
In considering one or more of these controls it is necessary to take into account any added risks
associated with them.
2.2.1 Elimination Control
•
Does the work actually require the use of human material?
2.2.2 Substitution Control
•
Consider using material from a screened source e.g. blood-bank blood.
•
Is it possible for the students/researchers to use material from their own body? This is
expressly forbidden when working with cell lines.
•
Consider using avirulent or attenuated microbial strains.
•
2.2.3 Engineering Control
• Work on human material is to be only carried out in a designated “Biohazard” facility, with
restricted access, and appropriate facilities and signage.
• Biological safety cabinets provide operator protection, rather than protection against crosscontamination of the material being handled (which is afforded by a laminar flow hood or
similar device).
• Systems/procedures that generate risks of cuts, puncture wounds, or other injuries must be
reviewed as to whether they can mechanised or otherwise modified to eliminate/reduce such
risks.
2.2.4 Administrative Control
• Local protocols and safe operating procedures.
• Local emergency procedures (for first aid, for spills, etc.).
• Local job induction and safety training.
• Detailed disinfection procedures.
• Equipment decontamination, repair and transfer procedures.
• In particular:
1. Care should be taken when opening or extracting from ampoules so as to avoid cuts.
2. Needles, syringes and sharps must NOT be re-sheathed but be disposed of into the
appropriate “sharps” containers, and never into refuse sacks.
3. Sharps disposal bins must not be filled above the level indicated. They must be
carefully sealed and securely stored prior to (regular) disposal so that those handling
them will not be put at risk.
4. An appropriate disinfectant solution should be used to clean any human-derived
material-contaminated surfaces.
SOP #13
Working with Human-Derived Material
with Specific Reference to the Risk of Needle Stick Injuries.
Page 3 of 4
2.2.5 Personal Measures/Hygiene Control
• Vaccination (see 2.4 below)
• Abrasions/eczema/raw areas or cuts to the skin must be covered with waterproof dressings and
gloves where appropriate.
• Smoking and eating is prohibited in all laboratory areas where clinical work is being carried
out.
• Hand washing after contact with human-derived material blood is always absolutely essential.
2.2.6 Personal Protective Equipment (PPE) and Clothing Control
First ensure that all PPE is appropriate to the task and hazards and fits well.
• Appropriate disposable gloves must always be worn whenever there is potential contact with
human derived material.
• Clothing contaminated with human derived material should be placed in a plastic bag for
cleaning. Such cleaning should not be conducted at home, but by an approved service supplier.
• Remove gloves before answering a telephone, using a pen, handling documents, light switches,
door handles, etc.
• Where there is a risk of specimen spraying, splashing or spillage wear appropriate eye and face
protection, such as goggles, mask or full face visor.
• Laboratory coats, preferably Howie type, must be worn at all times.
• Personal clothing (including footwear) must be appropriate to the working area. Sandals or
open-toe style footwear should not be worn.
2.3 Vaccination Procedure
Working with human-derived material involves a risk of contracting potentially fatal diseases many of
which are not vaccine preventable. However vaccines are available for hepatitis A (HAV) and
hepatitis B (HBV). Personnel should ensure that before commencing work with human tissues that
they have been vaccinated (and assessed in the case of HBV).
3. Accident Management
A significant exposure occurs in the following circumstances:
i. A skin cut or puncture by a sharp object which has been used on a patient, or has been in
contact with a patient’s blood, blood components or other body fluids.
ii. Aspiration or injection of blood, blood components or other body fluids.
iii. Splashing of blood, blood components of other body fluids into the face, particularly the lips,
mouth and eyes.
iv. Extensive splashing of blood, blood components or other body fluids over large areas of
unprotected body surface or clothing, or on a skin surface that is broken.
v. Bites and scratches that break the skin or mucous membranes.
SOP #13
Working with Human-Derived Material
with Specific Reference to the Risk of Needle Stick Injuries.
Page 4 of 4
3.1 First Aid Procedure
Procedures to be promptly followed in the event of a needle stick injury or other exposure accident
resulting in a puncture wound:
1. Wash the area thoroughly with soap and warm water. In the case of needle stick injury/wounds
encourage them to bleed.
• Do not suck the puncture site.
• Do not use a nailbrush.
Apply a sterile dressing before referral to the hospital Accident and Emergency department.
2. In the case of splashes to the eye(s) wash with clean water (use an eye-wash fountain) for 15-20
minutes.
3. In the case of splashes to the face, thoroughly irrigate the nose, mouth and skin with clean water.
3.2 Procedure for Referral to Accident and Emergency, University College Hospital.
1. Immediately bring the affected person to the A&E Department (or call an ambulance where
appropriate – the emergency number is 112/999).
2. At the A & E Department the situation will be assessed and arrangements made for blood
samples to be taken from the person exposed. The person will be asked to give their informed
written consent before blood samples are taken.
Do not give tetanus toxoid.
3. The person (or accompanying person) should have details of the victim’s hepatitis B
vaccination status2, and as much detail as possible on the human derived material involved in
the accident.
4. The person will be given appropriate “post-exposure prophylaxis” (hepatitis B vaccination,
immunoglobulin, anti-HIV medication) depending on the assessment of the risk.
5. Report the necessity of referral at once to the head of discipline where the accident occurred.
6. Counselling and follow-up is essential and should be provided.
3.3 Reporting and Notification Procedures
• A report of every accident / incident must be made to the head of discipline.
•
The head of discipline will immediately report every accident / incident to the Health and Safety
Office using the university Accident Report Form.
•
In the case of an employee who was infected prior to the commencement of university
employment, it is the duty of that individual to inform the head of discipline. The university shall
at all times act in such a way as to ensure appropriate confidentially for the employee.
2
Staff/Students should keep their vaccinations record in their possession while at work.
SOP # 14 : Operating Procedure for Biological Safety Cabinet
Page 1 of 2
Policy:
Biological Safety Cabinets (BSC) are provided and are to be used whenever there is risk of infection
by splatter or aerosolisation. All biological safety cabinets should be inspected and certified annually.
Purpose:
The policy ensures staff can safely manipulate potentially infectious specimens.
Responsibility:
Management and employees
Key Elements
• Ensure all staff are familiar with safe work practices and procedures in compliance with
established University policies and procedures
• Establish and maintain a maintenance schedule for the biological safety cabinets
Procedure:
Class II Biological Safety Cabinet
This is a ventilated cabinet for personnel, product and environmental protection which
provides inward airflow and HEPA-filtered supply and exhaust air. Class II cabinets are used for low to
moderate risk biological agents and for minute quantities of toxic chemicals.
Operating Procedure for Biological Safety Cabinet
"The 10 Commandments"
1.
•
•
•
•
Preparation
Turn off UV lamp (where fitted), turn on fluorescent
Check air grilles for obstructions, switch on blower
Allow air to purge workspace 5 minutes
Ensure air pressure is within allowable limits.
2. Disinfection
• Spray or swab all interior surfaces with appropriate disinfectant
• Allow to air dry
3.
•
•
•
•
Assemble Material
Introduce only material required to perform procedure
Place material such that clean and contaminated items do not meet
Place contaminated material container at right rear
Ensure view screen is properly located and secured
4. Purge (pre -use)
• Allow air purge period with no activity inside (leave blower on!)
SOP # 14 : Operating Procedure for Biological Safety Cabinet
5. Personal Procedures
• Don protective clothing, gloves, mask, etc. as appropriate
6. Perform Procedures
• Introduce hands into work space, work carefully and methodically (i.e. from clean to
work area to discard).
• DO NOT remove hands from work space until procedures are complete and all
critical material is secured.
• Remove gloves into contaminated material container
7. Purge (Post-use)
• Allow air purge period with no activity inside (leave blower on!).
8. Personal Procedures
• Remove protective clothing, mask, etc. dispose as appropriate.
• Wash hands.
9. Terminal Disinfection
• Don gloves, remove materials to incubator, etc., to biohazard bag, autoclave as
appropriate.
• Spray or swab all interior surfaces with appropriate disinfectant.
10. Shutdown
• Turn off blower and fluorescent lamp.
• Turn on UV lamp (where fitted).
Page 2 of 2
SOP # 16 : Emergency Wash Facilities
Purpose:
To reduce the possibility of personal injury.
Key Elements:
• Eyewash facilities
• Emergency showers
Procedure:
Eyewash
Facilities
Know location of eyewash facilities and how to use them.
If eye protection fails, go to eyewash facility:
Eyewash fountain (preferred)
• Press handle to activate. Plastic covers will pop off.
• Wash eyes / face continuously for 15 min, to prevent serious
damage to the eyes.
Eyewash
Facilities
Eyewash station
(Sterile Isotonic Eyewash Solution)
• Remove bottle from station and twist off cap, break seal.
• Hold a few inches above eye or affected body area and flush as
needed. Control flow rate by pressure on bottle.
• Move to eyewash fountain and continue flushing eyes / face or
continue flushing with bottles from other eyewash stations.
• Wash eyes / face continuously for 15 min, to prevent serious
damage to the eyes.
Seek medical attention immediately thereafter.
Eyewash fountains are at designated laboratory areas or hallways.
They must be inspected once weekly and documented. If possible,
eyewash fountains should be plumbed with tepid water.
Eyewash stations with Sterile Isotonic Eyewash Solution are at other
locations in the research and teaching laboratories . They contain a
bottles of sterile isotonic eyewash solution. They must be must be
inspected with respect to unbroken seal and expiration date. Replace
at expiration date. Do not reuse. Once opened, discard. Do not use if
seal is broken. Contents - A colorless solution containing dibasic
sodium phosphate, sodium chloride, sodium phosphate monobasic
preserved with disodium EDTA and benzalkonium chloride.
Page 1 of 2
SOP # 16 : Emergency Wash Facilities
Emergency
showers
Page 2 of 2
Know locations of emergency showers and how to use them. Every
laboratory must be equipped with or have ready access to a safety
shower.
In case of chemical burns, go to nearest shower.
• For ceiling shower, pull handle to activate.
• For hand-held shower, pull out and press handle to activate.
• Shower, washing affected areas, for 15 min.
• Seek medical attention immediately thereafter.
Ceiling shower must be inspected twice per year and documented
Hand-held showers must be inspected once weekly and documented
SOP # 17 : General health and safety information for fieldwork
page 1 of 5
General health and safety information for fieldworkers at the Applied Ecology
Unit, Discipline of Microbiology, NUI, Galway.
Compiled by Dr. Michael Gormally
This leaflet contains notes on good practice which you may find helpful before
undertaking fieldwork.
Access permission. Always obtain permission from the relevant landowners or tenant
to enter any private land before commencing fieldwork. Do not continue fieldwork if
access permission is later revoked. A letter confirming your participation in NUI,
Galway fieldwork can be provided on request.
Risk assessment. Before undertaking any activities, every fieldworker should consider
the particular health and safety hazards associated with their individual study sites and
whether their individual circumstances and medical conditions expose them to
particular hazards. Individuals should assess any potential risks arising from their
fieldwork activities, which should include considering the risks specific to individual
sites. You should think about what precautions should be taken against any risks. If you
have any concerns, please raise these with the NUI, Galway Health & Safety Office (ext
2678).
Health and safety reporting. Fieldworkers should pass on health and safety
information provided to them by NUI, Galway to other people helping them with NUI,
Galway - related activities. You are encouraged to report any particular health and
safety issues about the survey methods or the survey sites to the NUI, Galway Health &
Safety Office (ext 2678).
Mobile telephones. It is advisable to carry a mobile telephone, which may be useful in
case of an emergency. Please note that mobile telephones may not work in some remote
areas, and are only of any use if you are conscious and capable of operating them.
Lone working. Lone working is to be discouraged as far as possible. It can only take
place when all other options have been examined and risk assessment must be done in
conjunction with NUI, Galway Health & Safety Office (ext 2678). The minimum
requirements are that you always leave a note of your whereabouts with a responsible
person. This should include: date and time of departure, method of travel to and around
SOP # 17 : General health and safety information for fieldwork
page 2 of 5
the site, proposed itinerary, expected time of leaving the site and return to base, and
vehicle identification details. The field worker should make arrangements to contact the
responsible person at least every eight hours and at the end of the working day. The
person to whom these details are given should be told who to contact if you fail to make
scheduled contact or if you do not return and at what time to raise the alarm.
Working in remote areas.
• If going to a remote place, then always leave a note of your whereabouts with a
responsible person. This should include: date and time of departure, method of travel
to and around the site, proposed itinerary, expected time of leaving the site and return
to base, and vehicle identification details. The person to whom these details are given
should be told who to contact if you do not return and at what time to raise the alarm.
If possible, do not work alone.
• If working in mountains, moorlands or unpredictable and extreme
environments, hypothermia is a significant hazard. In such situations, it is
appropriate to wear footwear with good ankle support and to carry warm
and waterproof clothing. Carry a map and compass and know how to use
them. Carry a whistle*, torch and waterproof watch and, where
appropriate, a survival bag with extra high-energy food supplies (eg.
sweets, chocolate, glucose tablets or biscuits). A fair test of your clothing
and equipment is the answer to the question: could I survive, be it very
uncomfortably, if I were immobilised for 24 hours? High visibility
clothing is desirable in many situations, both to prevent accidental injury
and, more importantly, to be located in an emergency.
• Avoid or abandon outdoor activities in bad weather.
Working near waterbodies or in wetlands. Fieldworkers working near
waterbodies or in wetlands (eg. bogs, fens, callows, turloughs etc) should
wear a life jacket and should never work alone.
Fieldwork at night or in the evening. When working into the hours of
darkness, carry a torch and spare batteries. Where appropriate, inform local
Garda and other locals of your intended area of operations. Be extra vigilant
SOP # 17 : General health and safety information for fieldwork
page 3 of 5
at night and wear something bright or reflective when walking on roads. If
possible, do not work alone.
Livestock and agricultural machinery. Take special care when entering
areas with livestock, especially cattle, rams and horses. Do not enter fields
containing bulls and be especially cautious with farm dogs. Rutting deer can
also be aggressive in the autumn. Avoid undertaking fieldwork in close
proximity to working agricultural machinery or forestry operations.
Parking. Take care to park sensibly, preferably off-road. Ensure that
entrances are not blocked.
Terrain. Take special care when carrying out fieldwork along watercourses,
cliff edges, or in areas that contain boggy ground, reedbeds or loose rocks.
Wear bright-coloured clothing when carrying out fieldwork along busy
roads. Do not cross potentially hazardous sites, such as quarries, ravines and
railway lines and do not attempt to climb steep slopes, walls or fences.
Please heed warning signs and do not enter land that has been deliberately
obstructed by fencing or barbed wire.
Intertidal areas, including open mudflats and saltmarshes, are potentially
very hazardous. You should be particularly careful if you need to go below
the high water mark: check high tide times before commencing fieldwork
and allow ample time to leave the intertidal area. Remember that tides can
come in very quickly and that distances can be deceptive on wide, open tidal
flats. Small tidal creeks or flows can rapidly deepen on an incoming tide,
thus
cutting
off
an
apparently
safe
retreat.
Human confrontation. Consider your personal safety when conducting
fieldwork within the vicinity of known or likely trouble spots. Avoid
confrontation with landowners, land workers or members of the public.
Consider the privacy of residents when performing early-morning survey
work in residential areas. Carry some form of identification to confirm the
activities you are undertaking. If you have any concerns about your personal
safety, cease fieldwork immediately.
SOP # 17 : General health and safety information for fieldwork
page 4 of 5
Equipment. It is important to consider the safety aspects of any equipment
that is used and any associated hazards. Fieldworkers are advised to always
carry a basic first aid kit to dress any minor cuts and abrasions. It is
important to know how to use a first aid kit and first aid training is available
from the NUI, Galway Health & Safety Office (ext 2678).
Diseases. Fieldworkers may be exposed to disease during survey work. If a
disease is suspected, then it is important to inform your doctor that you may
have been exposed to diseases associated with outdoor activities. Typical
diseases that may be encountered are:
• Tetanus may result from the infection of even minor wounds and
scratches with Clostridium tetani, a common micro-organism in soil.
• Weil’s disease (leptospirosis) that can be fatal if left untreated. The
organism is carried by rats and excreted in their urine, and persists in
water such as in puddles and slow-moving rivers in rat-infested places.
Thus visits to sites where rats might occur may pose a risk.
• Lyme disease, a bacterial disease transmitted by animal ticks associated
with rank vegetation, which leads to severe symptoms if left untreated. A
variety of animals act as hosts for the bacteria, including sheep, deer and
pheasants. For more information, see: www.bada-uk.org or contact
BADA-UK (PO Box 70, North Walsham, NR28 0WX).
• Tick-borne Encephalitis, a viral disease carried by animal ticks. Warm
forested areas with heavy undergrowth give the greatest risk from ticks in
late spring and summer. A vaccine is available where prolonged exposure
in the risk areas is likely. In Britain & Ireland, a related virus responsible
for ‘Louping ill’, a disease with symptoms varying from a mild flu-like
illness to more severe disease requiring hospitalisation, can infect a wide
variety of mammal and bird hosts, particularly grouse and hares in
moorland regions.
Discipline of Microbiology, NUI Galway
SOP # 17 : General health and safety information for fieldwork
Safety Statement
page 5 of 5
Minimising the risk of such diseases requires straightforward actions. For
example:
• Immunisation against Tetanus and Poliomyelitis.
• Remove ticks from the skin as soon as possible: wear light-coloured clothing so
that ticks are visible, tuck trouser bottoms into socks so that ticks cannot attach or
climb up the leg, and make regular checks of skin and hair.
• Wear disposable plastic gloves at all times when handling any dead animals;
dispose of them responsibly after use. Hands should be thoroughly cleaned after
fieldwork. Cigarettes can also transfer the infection from hands to mouth.
• During fieldwork, cuts and abrasions should be kept covered by a dressing.
Medical conditions: Field workers receiving special medical treatment, such as a
course of injections, or suffering from medical conditions eg. diabetes, allergies, rare
blood groups etc. are reminded of the advisability of carrying a card or some other
indication of special medical requirements. Where applicable, sufficient additional
medicines, etc., should also be carried during field work. Field workers should complete
the NUI, Galway form indicating current medical conditions.
*Whistle: The International Alpine Distress Signal is six long whistle blasts or torch
flashes in succession repeated at 1 min intervals. The reply is three long whistle blasts
or torch flashes repeated at 1 min intervals.
Further information can be obtained from…
Guidance on Safety in Fieldwork (2005), Universities and Colleges Employers
Association, Woburn House, 20 Tavistock Square, London WC1H 9HU
(www.ucea.ac.uk)
Discipline of Microbiology, NUI Galway
Safety Statement
Fieldwork Risk Assessment Form
Title of Fieldwork Activity:
Location(s) of Work:
Duration (incl. dates From / To) :
Brief Description of Fieldwork:
Hazard Identification: Identify all the hazards; evaluate the risks (low / medium / high) and describe all necessary control
measures.
Hazard (s)
Physical Hazards (e.g. extreme
weather conditions, cliffs, caves,
mountains, marshes, quicksand,
fresh / seawater, mines, quarries,
tides)
Biological Hazards (e.g.
poisonous plants, venomous /
aggressive animals, soil or water
micro organisms, insects)
Chemical Hazards (e.g.
pesticides, dusts, contaminated
soils, chemicals on site)
Man-made hazards (e.g.
machinery, electrical equipment,
vehicles, insecure buildings,
slurry pits, power and pipelines)
Personal Safety (e.g. lone
working, attack on person or
property, first aid)
Risk
L/M/H
Control Measures
Discipline of Microbiology, NUI Galway
Safety Statement
Environmental impact (e.g.
refuse, pollution, disturbance of
eco-systems)
Other hazards (e.g. procedural,
manual handling) Please specify.
*Continue on separate sheet if necessary
Emergency Procedures: Specify arrangements for first aid, special emergency procedures, survival aids, communication,
etc.)
Additional Information: Identify any additional information relevant to the fieldwork activity, including supervision,
training requirements, information, specialist equipment or clothing, inoculations, etc.
Contact Information: Include details of both the University designated contact and on-site contact.
Name:
Tel. Contact:
University
Name:
Tel. Contact:
On-site
Address of residential base:
Has necessary training and information been given?
Yes
No
N/A
Is there adequate provision for those with health problems or
disabilities?
Yes
No
N/A
Are there adequate First Aiders available?
Yes
No
N/A
Discipline of Microbiology, NUI Galway
Safety Statement
Is there suitable supervision (i.e. Staff to Student ratio)?
Yes
No
N/A
Is permission required to work on site?
Yes
No
N/A
Are there suitable travel arrangements and licensed drivers?
Yes
No
N/A
Is adequate insurance cover in place?
(Contact Finance Office for advice, 50-9154)
Yes
No
N/A
Have all participants submitted next of kin information to
NUI, Galway?
Yes
No
N/A
Have route notification schedules been provided to the Garda
Síochána or Coastguard?
Yes
No
N/A
Assessment carried out by:
Name:
Signature:
Title (e.g. Group Leader, Lecturer, Research Student, etc):
Date:
Review Date:
Assessment Authorised by Head of School / Fieldwork Supervisor:
Name:
Date:
Signature:
DISCIPLINE OF MICROBIOLOGY
RISK ASSESMENT
OF
CHEMICAL AGENTS
Please see Section 12 for the key to understanding how Risk Assessments were carried out in
the following section
Manufacturer
Chemical name
Product Code
CAS Number
1-[3-(Dimethylamino)propyl]-3ethylcarbodiimide methiodide
165344
22572-40-3
Sigma
B 9673
109-70-6
Sigma
1990; 27500 6E
71-36-3
Merck; BDH
1289386
98-85-1
Merck
103456A; 29679
71-23-8
BDH
131377
66-71-7
Sigma
320056
5144-89-8
Sigma
E 4876
57-63-6
Sigma
274984
17455-13-9
Sigma
N 1000
90-15-3
sigma
284467
109-86-4
sigma
D 174904
576-26-1
Sigma
06720
926-39-6
1-Bromo-3-chloropropane
1-butanol (n-butanol)
1-phenylethanol
1-propanol
1,10 - Phenanthroline
1,10 - Phenanthroline
monohydrate
17 -Ethynylestradiol
18-Crown-6 Ether
Naphthol BDH 10162 5X
2 methoxy ethanol
2-6, dimethylphenol
2-Aminoethyl hydrogen sulphate
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
2.00
2.00
1.00
4.00
2.00
2.00
4.00
1.00
3.00
4.00
2.00
2.00
Manufacturer
Chemical name
Product Code
CAS Number
2-Bromoethanesulfonic acid Sod.
salt
137502
4263-52-9
36746
95-57-8
Sigma
K2755
103404-70-2
Sigma
K 40-1
600-18-0
Sigma
M 6250
60-24-2
245526
1730-91-2
Sigma
2-Methyl-24-pentanediol
(Hexylene Glycol)
M9671
107-41-5
Sigma
2-methylpropan-2-ol (Tert
butanol)
423041 B 2138
75-65-00
BDH
2-chlorophenol
2-keto-3-deoxyoctonate
ammonium salt
2-ketobutyric acid
2-Mercaptoethanol
2-methyl butyric acid
2-propanol (isopropanol)
2-thiobarbituric acid
2,2′-Azino-bis(3ethylbenzothiazoline-6-sulfonic
2,4 Dinitrophenol
2,4 Dinitrophenylhydrazine
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
3.00
1.00
1.00
4.00
152526S; 102246L 67-63-0
BDH
T5500
504-17-6
Sigma
A 1888
30931-67-0
D198501
51-28-5
D 199303
119-26-6
2.00
3.00
1.00
4.00
0.25
1.00
sigma
3.00
3.00
Chemical name
2,4-Dichloro-Phenoxyacetic Acid
2,4-Dichlorophenol
2,4,6-Trichlorophenol
222 Trifluoroethanol
(3-Carboxypropyl) Trimethyl
Ammonium Chloride
3-chlorobenzoic acid
3-hydroxybiphenyl
(±)-3-Methyl-2-oxovaleric acid
sodium salt
3,5-dinitrosalicyclic acid
4-Aminobenzoic Acid BDH
27103
4-bromophenacyl bromide
4-Fluorophenol
4-Nitrophenol
Manufacturer
Product Code
CAS Number
D 7299
94-75-7
Sigma
105953
120-83-2
Sigma
T 55301
88-06-2
Schunchardt
96924
75-89-8
Fluka
C 2686
6249-56-5
Sigma
C 24604
535-80-8
Sigma
262250
580-51-8
Aldrich
K 7125
3715-31-9
D 0550
609-99-4
Sigma
A 9878
150-13-0
Sigma
D 38308
99-73-0
Sigma
F 13207
371-41-5
Merck
1048
100-02-7
BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
4.00
4.00
4.50
1.00
1.00
1.00
0.50
1.00
4.00
1.00
2.00
1.00
Manufacturer
Chemical name
Product Code
CAS Number
5-Bromo-4-chloro-3-indolyl-B-DGalactopyranoside( X-Gal )
B 4252
7240-90-6
Sigma
5(6)Carboxyfluorescein, for
fluorescence
21877
72088-94-9
Fluka
6-Nitro-3(phenyl-acetamido)
benzoic acid
N 8883
52033-70-2
sigma
H 6878
148-24-3
Sigma
M 4252
2749-07-7
Sigma
T 3251
10191-41-0
Sigma
A 0500
60-35-5
sigma
A 6283
64-19-7
Sigma
A 6404
108-24-7
Sigma
179124
67-64-1
A 3396
75-05-8
Sigma
114189
75-36-5
BDH
24,085-0 221236
12054-85-2
Aldrich
8-HydroxyQuinoline
-Methyl-DL-Methionine
Tocopherol
Acetamide
Acetic Acid - 100% Glacial BDH
10001CU
Acetic anhydride
Acetone
Acetonitrile
Acetyl chloride
Acidified ammonium molybdate
4H2O
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
2.00
2.00
0.50
0.50
4.00
3.00
3.00
2.00
1.00
4.00
1.00
Chemical name
Acridine orange
Acrylamide
Acrylamide bis
Acrylamide/bisacrylamide
Acrylamide/bisacrylamide
Acrylamide/bisacrylamide soln
37:5:1
Adenine
Adenosine 5 triphosphate
disodium salt
Adipic acid dihydrazide
Agar Bacteriological (Agar No. 1)
Oxoid L11
Agarose Biogene 300-300
Albumin from bovine serum
Alcian blue 8GX
Manufacturer
Product Code
CAS Number
A 6014
10127-02-3
Sigma
A 9099
79-06-1
BDH
A 2917
MIXTURE
Bio rad
A 2917
MIXTURE
Biorad
A 9926
MIXTURE
Biorad
A 2917
MIXTURE
Bio rad
A 8626
73-24-5
Sigma
A 2383
34369-07-8
sigma
A 0638
1071-93-8
Sigma
A 7002
9002-18-0
Sigma
A 9539
9012-36-6
Sigma
A9418; A4287; 05473
9048-46-8
Sigma; Fluka
A3157
75881-23-1
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
8.00
8.00
8.00
8.00
8.00
2.00
0.50
0.50
0.05
0.05
0.50
0.50
Chemical name
Manufacturer
Product Code
CAS Number
180947
9005-38-3
713023 P 8361
9001-78-9
roche
BDH 27062,
109-57-9
Sigma Aldrich
IRA410
63181-94-2
Sigma
A 5710
100915-96-6
Sigma
A 1774
39831-55-5
A 6899
1336-21-6
Sigma
A 1542
631-61-8
sigma
A 6141
1066-33-7
Sigma
A 5666
12125-02-9
sigma
Ammonium ferric citrate BDH
27163
F 5879
1185-57-5
sigma
Ammonium heptamolybdate
tetrahydrate
10028; 149200; 1182025
12054-85-2
BDH; H&W; Merck
277908
13106-76-8
BDH
Alginic acid
Alkaline phosphatase
Allylthiourea thiosinamine
Amberlite IRA 410
Amberlite mb150
Amikacin
Ammonia soln. BDH 27141 5B
Ammonium Acetate BDH 27142
Ammonium Bicarbonate A-6141
Ammonium chloride BDH 27149
Ammonium molybdate
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
2.00
1.00
1.00
0.50
3.00
1.00
1.00
1.00
1.00
1.00
1.00
Manufacturer
Chemical name
Product Code
CAS Number
Ammonium Molybdate BDH
27187
A 7302
12054-85-2
sigma
A 9164
7727-54-0
sigma
Ammonium phosphate
( ammonium dihyrogen
A1645
7722-76-1
Ammonium sodium hydrogen
orthophosphate
S 4172
7783-13-3
BDH
Ammonium Sulphate BDH
100333B
A 2939
7783-20-2
sigma
A 2411
1397-89-3
Sigma
A 9518
69-52-3
Sigma
10038
71-41-0
BDH
10041 4B 242284
62-53-3
BDH
A8011
MIXTURE
Sigma
A 3256
5328-37-0
sigma
A 3256
87-72-9
sigma
A 5006
74-79-3
sigma
Ammonium persulphate
Amphotericin B
Ampicillin
Amyl alcohol (1-pentanol)
Aniline
Antifoam C emulsion
Arabinose L(+)
Arabinose L(+)
Arginine BDH 37190 4T
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
4.00
0.50
0.50
0.50
2.00
4.00
0.40
8.00
0.40
0.50
0.50
0.50
Manufacturer
Chemical name
Product Code
CAS Number
Arsenic acid potassium salt
(Potassium arsenate)
A 6631
7784-41-0
Sigma
A 5960
50-81-7
sigma
LS315
NONE
Nat Diag
N 5755
53-59-8 mix
Sigma
T 8910
139-86-6
-Nicotinamide adenine
dinucleotide
N 8285
53-84-9
Bacto Yeast Extract Difco
212750
Y 1625
8013-01-2
sigma
612420
144-02-5
BDH
B0750
10326-27-9
Sigma
Ascorbic Acid L(-) BDH 44006
Autofluor
Nicotinamide adenine
dinucleotide phosphate
-(2-Thieny)-DL-Alanine
Barbitone sodium
Barium chloride dihydrate
BBL INDOLE (reagent) 261187
261187 D8904
BBL OXIDASE (reagent)4361181
4361181 T3134
NNN'N' Tetramethyl-p-phenyleneBeef extract
Benzene
mix with 11%
637-01-4
B 4888
NONE
319953
71-43-2
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
0.05
4.00
2.00
1.00
0.50
BBL
bbl
0.50
1.00
2.00
2.50
0.25
0.50
Merck
4.00
Chemical name
Benzlypenicillin sodium salt
Benzoic acid
Beta-(2-Thienyl)-DL-Alanine
Beta-naphthyl propionate (2naphthyl propionate)
Betaine
Betaine aldehyde chloride
Betaine anhydrous
Bile Salts No. 3 Oxoid L56
Bisbenzimide
Bismuth (III) Citrate
BisTris 2-2-Bis-(hydroxymethyl)
-2,2',2''-nitrilotriethanol
Boric Acid (Orthoboric Acid )
Brain Heart infusion (oxoid
CM225)
Manufacturer
Product Code
CAS Number
P 3032
69-57-8
sigma
B 9300
65-85-0
Sigma
T 4875 287288
2021-58-1
Sigma Aldrich
N 0501
13080-43-8
Sigma
14300 B2754
590-47-6
sigma
B 3650
7758-31-8
Sigma
B 2629
107-43-7
Sigma
B 3301
NONE
Sigma
B 2261Hoechst
23491-52-3
B 2261
480746
813-93-4
Sigma
B 9754
6976-37-0
B 7660
10043-35-3
Sigma
B 7403
NONE
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
1.00
0.50
0.50
0.50
0.50
1.00
2.00
1.00
1.00
4.00
0.50
Chemical name
Brefeldin A
Brilliant blue G
Brilliant blue R
Bromocresol purple BDH 20014
5F (-25g)
Bromophenol blue BDH 20015
Bromothymol blue BDH 20020
4W (-25g)
Product Code
CAS Number
B6542
20350-15-6
Sigma
B0770
6104-58-1
Sigma
B 7920
6104-59-2
B 5880
115-40-2
sigma
B 0126
115-39-9
sigma
114413
76-59-5
Aldrich
877-24-7
Fisher
B 7906
71-36-3
sigma
B 103500
107-92-6
Sigma
265365
7440-43-9
BDH
C 3141
7790-78-5
Sigma
202894
1306-19-0
BDH
C 3032
7647-17-8
BDH
Buffer Soln Phthalate
Butanol
Butyric acid
Cadmium
Cadmium chloride
Cadmium oxide
Caesium chloride
Manufacturer
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
6.00
0.50
0.75
1.00
0.50
0.50
0.50
3.00
1.00
4.00
4.00
4.00
0.50
Chemical name
Calcium Carbonate BDH 27579
Calcium chloride anhydrous
Calcium Chloride dihydrate
Calcium chloride hexahydrate
Calcium hydrogen
orthophosphate BDH 27598
Calcium nitrate
Calcium oxide BDH 27614 4R
Carbazol
Carbenicillin
Carbol fuchsin solution
Casamino Acids Difco 223050
casein from bovine milk
Catalase
Catechol
Manufacturer
Product Code
CAS Number
C 6763
471-34-1
Sigma
C 4901 C2661
10043-52-4
BDH
C 3881
10035-04-8
Sigma
21108
7774-34-7
Fluka
C 7263
7757-93-9
sigma
C 4955
13477-34-4
Sigma
L 7161
1305-78-8
C5132
86-74-8
C1389
4800-94-6
21820
mixture
-
C 7078
9000-71-9
Sigma
C 9322
9001-05-2
Sigma
452637
17763-91-6
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
1.00
1.00
1.00
0.50
2.00
3.00
Sigma
4.00
2.00
4.00
0.50
0.50
1.00
Chemical name
Cefotaxime
Cefoxitin
Celestin blue b HW 007300 CI
51050
Cephalexin
Cephalosporin c Zinc salt
Cephradine
Cetylpyridinium chloride
CHAPS
Charcoal (activated, decolorizing)
Chenodeoxycholic acid
Chloramphenicol
Chlorine
Chloroform BDH 10077
Manufacturer
Product Code
CAS Number
C 7912
64485-93-4
sigma
C 4786
33564-30-6
Sigma
206342
1562-90-9
sigma
C 4895
15686-71-2
22237
59143-60-1
Fluka
C 8395
38821-53-3
sigma
27921 2B C 0732
6004-24-6
BDH
C 5070
75621-03-3
Sigma
330324E 161551
7440-44-0
BDH
22510
474-25-9
C 7795
56-75-7
Sigma
295132
7782-50-5
Fisher Scientific
C 2432
67-66-3
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
4.00
0.50
4.00
4.00
4.00
3.00
1.00
0.20
0.75
4.00
3.00
4.00
Chemical name
Chlorohexidine
Chromium (III) chloride
Chromium (lll) nitrare
nonahydrate
Chromium potassium sulphate
Cinoxacin
Cis-1,2-Dichloroethylene
Citric Acid BDH 10081
Citric Acid, anhydrous BDH
2798444E
Product Code
CAS Number
C 9394
18472-51-0
Sigma
C1896 230723
10060-12-5
Aldrich
239259
7789-02-8
243361
7788-99-0
C 8645
28657-80-9
D 62004
156-59-2
C 0706
5949-29-1
Sigma
C 1857
77-92-9
sigma
10026-22-9
Sigma
C 2644
7791-13-1
sigma
229598
60459-08-7
Sigma
449776
7646-79-9
BDH
C 3915
64-86-8
Sigma
Cobalt (11) nitrate hexahydrate
Cobalt (II) Chloride 6-hydrate
BDH 277902M
Cobalt sulfate
Cobaltous chloride
Colchicine
Manufacturer
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
1.00
3.00
BDH
0.50
0.50
2.00
2.00
2.00
4.00
4.00
8.00
4.00
3.00
Chemical name
Colistin sodium methanesulfonate
Colistin sulfate
Collagen from calf skin
Congo Red
Coomassie brilliant blue R-250
Copper (II) chloride 2hydrate
Copper (II) Sulphate anhydrous
Hopkin & Williams 347200
Creatinine
Cresol red BDH 20029
Crystal Violet
Cupric Chloride
Cupric sulphate (Copper (II) ) 5
H2O
Cyclohexamide
Manufacturer
Product Code
CAS Number
C1511
8068-28-8
Sigma
C4461
1264-72-8
Sigma
C9791
9007-34-5
Sigma
343222Q C6767
573-58-0
BDH
B 7920 161 0400
6104-59-2
BioRad
C 6641 221783
10125-13-0
Sigma
C 1297
7758-99-8
sigma
C 6257
19230-81-0
Sigma
114472
1733-12-6
C0775
548-62-9
Sigma
C 6641
10125-13-0
Sigma
209201
23254-43-5
BDH
C 7698
66-81-9
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
4.00
1.00
4.00
0.75
3.00
2.00
0.50
1.00
4.00
3.00
2.00
2.00
Chemical name
Cyclohexanol
Cystathionine
Cysteine (DL-)
Cysteine (L-) HCl anhyd BDH
37055
Cytosine
D (-) fructose
D (-) Ribose
D (+) GalactoseBDH 38026
D (+) Glucose
D (+) Sucrose
BDH 100935V
D-(+)-Galactosamine
D-(+)-Glucosamine
D-(+)-Mannose
Manufacturer
Product Code
CAS Number
29100
108-93-0
C 3633
535-34-2
861677
3374-22-9
sigma
C 1276
52-89-1
sigma
C 3506
71-30-7
Sigma
F0127
57-48-7
sigma
R 7500
50-69-1
Sigma
G 0750
59-23-4
sigma
G7528
50-99-7
Sigma; BDH; H&W
S 5016
57-50-1
sigma
G0264
1772-03-8
Sigma
G4875
66-84-2
Sigma
M4625
3458-28-4
Sigma
BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.50
2.00
1.00
1.00
0.05
0.50
0.05
0.20
0.05
0.50
0.50
0.50
Chemical name
D-(+)-Xylose
d-Biotin (Vit B7)
D-galactouronic acid
monohydrate
D-glucuronic acid
D-Histidine hydrochloride
D-Mannosamine
D-Pantothenic Acid hemicalcium
salt
D-Phenylalanine
Dabco LV 33 1,4-Diazabicyclo
[2.2.2]octane solution
Decane
Dent
Deoxycholic acid
Deoxyribonuclease 1
Manufacturer
Product Code
CAS Number
30590 X 1500
58-86-6
BDH
B 4501
58-85-5
Sigma
G2125
91510-62-2
Sigma
G 5269
6556-12-3
sigma
H7625
6341-24-8
Sigma
M4670
5505-63-5
Sigma
P 2250
137-08-6
Sigma
P 1751
673-06-3
Sigma
290734
280-57-9 mix
aldrich
D90,1 46154
124-18-5
SR0147
MIXTURE
Oxoid
D 5670
145224-92-6
Sigma
DN 25
9003-98-9
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.05
0.50
0.50
0.50
0.50
0.50
0.50
0.50
2.00
2.00
1.60
1.00
0.50
Chemical name
di Ammonium hydrogen
orthophosphate 27173
Product Code
7783-28-0
BDH
di-Ammonium Iron (II) Sulphate-6F 1543
hydrate BDH 100223T
7783-85-9
Sigma
di-Potassium hydrogen
orthophosphate BDH 104363A
P 5504
16788-57-1
sigma
di-Potassium phosphate
K2HPO4
104363A P 3786 7758-11-4
BDH
di-Sodium hydrogen
orthophosphate anhydrous BDH
S 9763
7558-79-4
sigma
Dichloromethane (methylene
chloride)
6054
75-09-2
Merck
D76609
461-58-5
Sigma
Dicyandiamide
Diethyl ether
Diethyl Pyrocarbonate
Diethylaminoethyl cellulose
Diiodomethane
Dimethyl phthalate
Dimethylformamide BDH 10322
A 5764
CAS Number
Manufacturer
10094 6B 346136 60-29-7
Sigma; BDH
D 5758
1609-47-8
Sigma
I 6505 30477
9013-34-7
Whatman
66880
75-11-6
Sigma
w508500
131-11-3
BDH
D 4551
68-12-2
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
1.00
0.50
0.50
0.50
1.60
0.50
2.00
1.00
0.50
1.00
0.50
4.00
Chemical name
Diphenylamine
Disialoganglioside GD3 from
bovine milk
Disialoganglioside-GD2
disodium hydrogen
orthophosphate 2H2O
Dithioerythritol
Dithiothreitol
DL Asparagine
DL-6,8-Thioctic Acid or
acid
Lipoic
Product Code
CAS Number
10101 D 2385
122-39-4
BDH
G5904
62010-37-1
Sigma
G0776
65988-71-8
Sigma
71644
10028-24-7
BDH
D 8255
6892-68-8
sigma
D 5545
3483-12-3
Sigma
11180
3130-87-8
Sigma
T 5625
1077-28-7
Sigma
DL- , -Diaminopimelic acid ( 2,6D 1377
Diaminoheptanediocic acid )
583-93-7
DL- , -Diaminopimelic acid ( 2,6D 1377
Diaminoheptanediocic acid )
2577-62-0
DL-Histidine
DL-Homocysteine
DL-Homocysteine Thiolactone
Manufacturer
H 7750
4998-57-6
H 4628
454-29-5
H 8762
14007-10-4
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
6.00
0.20
0.20
0.50
1.00
1.00
1.00
1.00
1.00
1.00
Sigma
0.50
0.50
Sigma
0.50
Chemical name
DL-Homocystine
DL-Homoserine
DL-Isocitric Acid
DL-Isoleucine
DL-Malic Acid
BDH 29127
DL-Methionine
DL-Phenylalanine
DL-Serine BDH 6746520
DL-Threonine
DL-Tryptophan
DL-Tyrosine
DL-valine
DMSO (dimethylsulfoxide)
Manufacturer
Product Code
CAS Number
H 0501
870-93-9
Sigma
H 1001
1927-25-9
Sigma
I 1252
1637-73-6
Sigma
I 6268
443-79-8
Sigma
M 0875
6915-15-7
Sigma
M 9500
59-51-8
Sigma
P 4905
150-30-1
S 5386
302-84-1
sigma
T 8375
80-68-2
Sigma
T 3300
54-12-6
Sigma
145726
556-03-6
Fluka
94640
516-06-3
Sigma
D8418; 103234L
67-68-5
Sigma; BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
0.50
0.50
3.00
0.50
0.50
0.50
0.50
0.50
1.00
0.50
0.50
Chemical name
Manufacturer
Product Code
CAS Number
324378
14377-97-5
calbiochem
69011-19-4
Sigma
dpxmountant
BDH
E 2271
96702-03-3
Sigma
EDTA
(ethylenediaminetetraacetic acid)
E9884
60-00-4
Sigma
EGTA (ethylene glycol bis-baminoethyl NNNN-tetraacetic
E4378
67-42-5
Sigma
E 6376
114-07-8
Sigma
E 1253
50-27-1
E 9750
53-16-7
E 9129
107-21-1
BDH
E 7023
64-17-5pure
sigma
411000
141-43-5
E 7637
1239-45-8
Domoic acid
DOWEX-1, chloride form, strongly
1X2-400
basic, mesh 200-400
DPX mountant
Ectoine
Erythromycin
Estriol
Estrone
Ethanediol (ethylene glycol)
Ethanol MERCK 100983-2500
Ethanolamine
Ethidium Bromide
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.10
4.00
0.50
1.00
0.50
0.50
4.00
4.00
1.00
10.00
3.00
Sigma
4.00
Chemical name
Manufacturer
Product Code
CAS Number
E 7637
1239-45-8
Sigma
E 4391
1239-45-8
Sigma
270989
141-78-6
E 1769
25952-53-8
Sigma
112909
94-09-7
Fluka
284467
109-86-4
sigma
Ethylenediaminetetra-acetic acid
disodium salt
E 5134
6381-92-6
Sigma
Ethylenediaminetetra-acetic acid
ferric monosodium salt BDH
100935V
15708-41-5
BDH
Ethylenediaminetetraacetic acid
(EDTA) sodium salt solution
46081
139-33-3
BDH
D 9805
14263-94-6 +
Sigma
F 2877
10025-77-1
sigma
F 3388
3522-50-7
Sigma
28381
2338-05-8
Ethidium Bromide aqueous soln
Ethidium Bromide Tablets
Ethyl acetate
ethyl-3-cardodiimide
Ethyl-4-aminobenzoate
Ethylene glycol monomethyl ether
Fast Blue B salt
Ferric (Iron 111)chloride BDH
10110
Ferric Citrate
Ferric citrate
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
4.00
1.00
2.00
0.50
4.00
0.50
1.00
0.50
4.00
2.00
0.50
0.50
Manufacturer
Chemical name
Product Code
CAS Number
Ferrous sulphate (7H20) BDH
28400 5E
F 7002
7782-63-0
sigma
F3385
9014-81-7
Sigma
F 2637
26873-85-8
Sigma
F6625
84366-81-4
Sigma
27072-45-3
sigma
F 7250
3326-32-7
sigma
F 3543
4404-43-7
Sigma
F 9252
MIXTURE
BDH
F9252
mixture
Sigma
F 8775
50-00-0
sigma
F 4761
75-12-7
sigma
F-1900-PB08
64-18-6
Fisher
47860
58969-01-0
sigma fluka
Fetuine
Ficoll
Flavin Adenine dinucleotide
disodium
Fluorescein 5(6)-isothiocyanate
Fluorescein isothiocynate
Fluorescent Brightener 28
Folin and Ciocalteau phenol
reagant
Folin-Ciocalteau reagent
Formaldehyde BDH 10113
Formamide BDH 28422
Formic Acid
Fuchsin basic Gurr BDH 34032
F 3651
46950
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.50
0.50
0.50
4.00
4.00
1.00
3.00
3.00
8.00
4.00
3.00
4.00
Chemical name
Manufacturer
Product Code
CAS Number
A 2129
56-12-2
G 9382
9000-70-8
G 3632
1405-41-0
49180
9001-37-0
G7776; 49633
111-30-8
G 4251
70-18-8
150568
27025-41-8
Glyceraldehyde 3 phosphate
solution
G 5251
591-59-3
Glyceraldehyde 3 phosphate
dehydrogenase
G 2267
9001-50-7
Sigma
10118 6M
56-81-5
BDH
3033-77-0
Sigma
G 7126 (H5073)
56-40-6
sigma (Promega)
G 3272
50-01-1
Sigma
aminobutyric acid
Gelatin Oxoid L8 117F-0197
Gentamycin Sulfate salt
Glucose oxidase
Glutaraldehyde
Glutathione ( L-glutathione)
Glutathione (oxidised)
Glycerol BDH 10118 6M
Glycidyltrimethylammonium
chloride
Glycine BDH 28458 4L
Guanidine
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
Sigma
0.05
4.00
4.00
Sigma; Fluka
4.00
0.50
Calbio
0.50
2.00
0.50
0.05
4.00
0.05
1.00
Chemical name
Guanidine thiocyanate
Guanine
Guanosine
Product Code
CAS Number
G 7028
593-84-0
Sigma
G 6779
73-40-5
Sigma
G 6752
118-00-3
Sigma
9000-01-5
Sigma
H 2625
9008-02-0
sigma
H 7523
7365-45-9
Sigma
H0763
75277-39-3
Sigma
H3500
506-12-7
Sigma
H4515
1731-92-6
Sigma
H 6269
57-09-0
Sigma
H 3762
107-46-0
Sigma
604-44-4
BioRad
10034-93-2
Sigma
Gum Arabic
Hemoglobin Bovine freeze dried
bd 212392
HEPES
HEPES (sodiun salt)
Heptadecanoic acid
Heptadecanoic acid methyl ester
Hexadecyltrimethyl-ammonium
bromide(CTAB)
Hexamethyl disiloxan
HRP Color Development Reagent
170-6534 C8890
(4-Chloro-1-naphtol)
Hydrazine
Manufacturer
216046
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
1.00
0.50
0.50
0.50
0.50
1.00
0.50
3.00
1.00
2.00
4.00
Manufacturer
Chemical name
Product Code
CAS Number
Hydrochloric Acid 32.36 M BDH
101254H
H 7020
7647-01-0
380105
7664-39-3
H 1009
7722-84-1
sigma
H 9003
123-31-9
Sigma
H 0252
1306-06-5
Sigma
55243
165542-15-4
Fluka
431362
5470-11-1
Sigma
214906
6303-21-5
H 9377
68-94-0
72779
3380-34-5
I 5513
288-32-4
Sigma; Aldrich
I 0765
NONE
Sigma
458600
64-17-5IMS
Hydrofluoric acid
Hydrogen peroxide
Hydroquinone
Hydroxyapatite
Hydroxyectoine
Hydroxylamine HCl
Hypophosphorous Acid 50%
solution
Hypoxanthine 1117220
Igrasan
( triclosan )
Imidazol
Immersion Oil
Industrial Methylated Spirit
(Bench Alcohol)
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
8.00
2.00
4.00
0.50
1.00
4.00
2.00
BDH
0.50
4.00
6.00
0.50
12.00
Chemical name
Manufacturer
Product Code
CAS Number
I 5125
87-89-8
sigma
I 3380
7553-56-2
BDH
A 3221
144-48-9
Amersham
I4386
64-69-7
Sigma
101168123
74-88-4
Sigma
Iodomethane solution (in tertbutyl methyl ether cas1634-04-4)
67695
74-88-4 mixture
Fluka
IPTG Isopropyl -D-1thiogalactopyranoside
I 6758
367-93-1
sigma
C 3518
7439-89-6
Labor. Chem
380024
13478-10-9
BDH
436011
13463-10-0
BDH
451649
7705-08-0
Sigma
21682-8 F3002
7782-61-8
Sigma
I 9392
123-51-3
Sigma
Inositol BDH 38044
Iodine N 0.5M Solution BDH
19077 4V
Iodoacetamide RPN6302V
Iodoacetic acid
Iodomethane
Iron ( Carbonyl Iron )
iron (II) chloride 4-hydrate
Iron (III) orthophosphate
Iron chloride anhyd.
Iron(111) Nitrate
Iso Amyl Alcohol BDH 10038 5N
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.05
3.00
4.00
3.00
3.00
3.00
0.50
1.00
1.00
1.00
3.00
3.00
2.00
Chemical name
Isobutyric acid
Isonicotinic Acid Hydrazide
Isopropyl Alcohol (2-Propanol)
Kanamycin
106 801)
(Sulfate Boehringer
Kanamycin sulfate
Kieselgur
L Glutamic acid sod salt
L-(-)-Fucose
L-
aminobutyric acid
L-Alanine
L-Aspartic Acid
L-Beta-Phenylalanine BDH
2460230
L-Carnitine HCl
Manufacturer
Product Code
CAS Number
I 1754
79-31-2
Sigma
I 1137
55-22-1
Sigma
I 9516
67-63-0
Sigma
K 4000
25389-94-0
sigma
60615
70560-51-9
sigma
91053-39-3
Merck
G 5917
142-47-3
BDH
851388 F 2252
2438-80-4
Aldrich
A 1879
1492-24-6
A 7627
56-41-7
A 9256
56-84-8
Sigma
P 2126
63-91-2
sigma
C 0283
6645-46-1
10601
60779
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
1.00
4.00
4.00
4.00
3.00
0.50
0.50
4.00
0.50
1.00
0.50
1.00
Chemical name
L-Cysteine
L-Cysteine HCl
L-Cystine
L-Glutamic Acid
L-Glutamine
L-Histidine
L-Histidine HCl
L-Isoleucine
L-Leucine Hopkin & Williams
516600
L-Lysine
L-Malic Acid
L-Methionine BDH 6494090
L-Norleucine
Manufacturer
Product Code
CAS Number
C7755
52-90-4
Sigma
C 7880
7048-04-6
Sigma
C 8755
56-89-3
Sigma
G 1251
56-86-0
Sigma
G 3126
56-85-9
Sigma
H 8000
71-00-1
Sigma
H 8125
5934-29-2
Sigma
I 2752
73-32-5
Sigma
L 8000
61-90-5
sigma
L 5501
56-87-1
Sigma
M 9138
138-09-0
Sigma
M 9625
63-68-3
Sigma
N 6877
327-57-1
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
1.00
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
0.50
1.00
Chemical name
L-Proline
L-Proline
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine BDH 37156
L-Tyrosine Hydroxamate
L-valine
Lactic acid
Lactose BDH 10139 4S
Lauryl sulphate(SDS)
Lead acetate BDH 10143
Lead chloride
Manufacturer
Product Code
CAS Number
P 0380
147-85-3
171824
609-36-9
S 4311
56-45-1
Sigma
T 8625
72-19-5
Sigma
T 0254
73-22-3
Sigma
T 3754
60-18-4
Sigma
T 1380
4985-42-6
Sigma
V 0500
72-18-4
Sigma
L-0100-PB08
50-21-5
Fisher
L 8783
5989-81-1
sigma
L 4390
151-21-3
Sigma
L 3396
6080-56-4
sigma
203572
7758-95-4
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
0.50
0.50
0.50
1.00
1.00
0.50
1.00
0.05
2.00
4.00
4.00
Chemical name
Lecitin fromConcanavalin A
Leucine
Levofloxacin
Liquid paraffin BDH 29436 5H
Lithium bromide
Lithium chloride BDH 29067 4B
Lithium Dodecyl sulphate
Lithium Hydroxide
Litmus BDH 20060
Lomefloxacin
Luciferase
Luminol Reagent NEB
Lysine L(-) mono HCl BDH
37129 3P
Manufacturer
Product Code
CAS Number
23456
11028-71-0
calbiochem
L 7875
328-39-2
Sigma
28266
100986-85-4
rdh 18512
8012-95-1
Rdh
213225
7550-35-8
Sigma
L 8895
7447-41-8
sigma
L 9781
2044-56-6
Sigma
L 4533
1310-66-3
Sigma
L 7382
1393-92-6
sigma
L 2906
98079-52-8
L 4899
NONE
A 8511
521-31-3
sigma
L 5626
657-27-2
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
0.50
4.00
1.00
1.00
1.00
3.00
2.00
0.50
1.00
0.50
1.00
0.50
Chemical name
Lysostaphin
Lysozyme
Lyticase
m-Fluoro-DL-Phenylalanine
Magnesium chloride
Magnesium Chloride 6-hydrate
BDH 101494V
Magnesium oxide
Magnesium Phosphate di basic
3H20
Magnesium sulfate heptahydrate
Magnesium sulphate
Malachite green BDH 34045
Maleic Acid
Maltose
Manufacturer
Product Code
CAS Number
L7386
9011-93-2
Sigma
L 6876
12650-88-3
Sigma
L 2524
37340-57-1
F 5126
2629-54-1
Sigma
M 8266
7786-30-3
BDH
M 2670
7791-18-6
sigma
203718 243388
1309-48-4
Sigma
M 1265
7782-75-4
M9397; 10151
10034-99-8
Sigma; BDH
M 7506
7487-88-9
BDH
M 9015
2437-29-8
sigma
M 0375
110-16-7
Sigma
M 5885
6363-53-7
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.05
0.50
4.00
1.00
0.50
0.50
0.50
0.50
0.02
0.50
4.00
2.00
0.50
Manufacturer
Chemical name
Product Code
CAS Number
Manganese(11) (Manganous)
chloride BDH 29137
M 9522
13446-34-9
sigma
29146
10101-68-5
BDH
M 7634
10034-96-5
Sigma nearest 10034-96
-5
M 9647
69-65-8
Sigma
M 1136
7487-94-7
sigma
200093
7783-35-9
Sigma
M 4128
592-85-8
Sigma
M 3885
71119-23-8
Sigma
M 3671
4432-31-9
Sigma
69892
145224-94-8
Sigma
M 1775
67-56-1
Sigma
51454
132-92-3
M 3501
298-81-7
Manganous Sulphate 4H20
Manganous Sulphate mono H2O
BDH 10153
Mannitol BDH 10330 4Y
Mercuric iodide BDH 29165
Mercuric sulphate
Mercuric thiocyanate
MES
MES
MES 2-morpholinoethanesulfonic
acid
Methanol
Methicillin sodium
Methoxypsoralen
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
2.00
3.00
0.05
4.00
4.00
4.00
0.50
0.50
1.00
10.00
4.00
Sigma
4.00
Chemical name
Manufacturer
Product Code
CAS Number
M 7267
493-52-7
sigma
Methyl violet BDH 34033 5M CI
42555
C 3886
548-62-9
sigma
Methylene blue BDH34048 ci
52015
M 9140
7220-79-3
sigma
M3761
443-48-1
Sigma
ZWCL01F50
124-04-9
Millipore
M 8410
8042-47-5
sigma
M0753
1313-27-5
Sigma
G7641
37758-47-7
Sigma
M 1254
1132-61-2
Sigma
M 4782
55466-22-3
Sigma
N-(3-dimethylaminopropyl)nethylcarbodiiamide
E 6383
25952-53-8
Sigma
N-1-(Naphthyl)ethylenediamine
dihydrochloride
222488
1465-25-4
Sigma
A 7250
616-91-1
Sigma
Methyl red BDH 20067 ci 13020
Metronidazole
Millipore chlorine tablets
Mineral oil
Molybdenum trioxide
Monosialoganglioside GM1 from
bovine brain
MOPS
Mutanolysin
N-Acetyl-Cysteine
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
4.00
2.00
4.00
0.40
0.50
3.00
0.20
1.00
0.50
2.00
1.00
0.50
Chemical name
N-acetyl-D-galactosamine
N-acetyl-d-glucosamine
N-acetylneuraminic acid
n-Hexane
N-HYDROXYSUCCINIMIDE
N-Lauroylsarcosine sodium salt
N-nitroso-N-methyl-urea
N-phenyl-1-napthylamine,
Reagent Grade
n-Propyl gallate
n-UNDECYL-β-DMALTOPYRANOSIDE
N-Z-Amine
n,n-dimethyl 1,4
phenylenediamineoxalate
N,N-Dimethyl-1-naphthylamine
Manufacturer
Product Code
CAS Number
A 2795
1811-31-0
Sigma
A 8625
7512-17-6
Sigma
A2388
131-48-6
Sigma
34859
110-54-3
Sigma
130672
6066-82-6
Sigma
L 5125 L 9150
137-16-6
Sigma
N1517
684-93-5 MIX
Sigma
104043
90-30-2
Sigma
P 3130
121-79-9
Fluka
U300
253678-67-0
Affymetrix
C 7290
91079-40-2
409758
62778-12-5
Sigma
D4011
86-56-6
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
0.40
4.00
0.50
2.00
4.00
0.60
4.00
0.50
0.50
4.00
3.00
Chemical name
Manufacturer
Product Code
CAS Number
D4551
68-12-2
A 2917
110-26-9
160928186
74124-79-1
Sigma
N8878
389-08-2
Sigma
N 9005
134-32-7
Sigma
N1876
1405-10-3
Sigma
3345148
MIXTURE
BDH
Neuramidase from Clostridium
perfringens
N3001
9001-67-6
Sigma
Neutral red BDH 34056 4A CI
50040
N 7005
553-24-2
sigma
N 3376
98-92-0
Sigma
451193
7718-54-9
BDH
N 6136
7791-20-0
Sigma
N 4882
10101-97-0
N,N-dimethylformamide
N,N'-methylenebisacrylamide
N;N’-Disuccinimidyl carbonate
Naladixic acid
Naphthylamine BDH 10164 2R
Neomycin sulphate
Nessler's Reagant
Niacinamide (Nicotinamide)
Nickel (II) chloride
Nickel (II) chloride hexahydrate
Nickle (ll) sulphate hexahydrate
Sigma
79-06
BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
2.00
4.00
4.00
4.00
4.00
0.20
0.50
1.00
4.00
4.00
4.00
Chemical name
Nickle (ll) sulphate hexahydrate
Nicotinamide adenine
dinucleotidesodium salt
Nicotinic acid
Ninhydrin
Nitric Acid
10654732
Nitrilotriacetic Acid
Nitrilotriacetic Acid (Trisodium
Salt)
Nitrocefin
Nitrotetrazolium blue chloride
NNN'N' Tetramethyl p phenylene
sigma T3134
Norepinephrine bitartrate salt
Norfloxacin
Novobiocin sodium salt
Manufacturer
Product Code
CAS Number
N 4882
10101-97-0
N 0632
20111-18-6
N 4126
59-67-6
N4876
485-47-2
Sigma
N-2300-PB17
7697-37-2
Fisher
N 9877
139-13-9
Sigma
N 0253
5064-31-3
Sigma
484400
41906-86-9
Calbiochem
N6876
298-83-9
Sigma
T 3134
637-01-4
Sigma
A 0937
3414-63-9
Sigma
N 9890
70458-96-7
N6110
1476-53-5
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
1.00
1.00
3.00
3.00
4.00
1.00
1.00
1.00
4.00
0.50
Sigma
4.00
Chemical name
o-fluorobenzoic acid
O-Succinyl-Homoserine
Okadaic acid
Ornithine BDH 37191
Orthophosphoric acid
Oxalic acid
Oxolinic acid
Oxytetracycline HCl
p-Amino-DL-Phenylalanine
p-hydroxy phenylacetic acid
p-hydroxybenzoic acid
p-Nitrophenyl phosphate tablets
p-Phenylenediamine
Manufacturer
Product Code
CAS Number
412244
445-29-4
Sigma
S 7129
1492-23-5
Sigma
O 7760
209266-80-8
O 2375
3184-13-2
sigma
29420; 6024
7664-38-2
BDH; J.T.Baker
241172
144-62-7
BDH
O 0877
14698-29-4
sigma
O 5785
2058-46-0
sigma
A 1505
2922-41-0
Sigma
H 50004
156-38-7
Sigma
H 9882
5351-23-5
Sigma
P 4744
4264-83-9
Sigma
P6001
106-50-3
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.50
2.00
0.05
2.00
1.00
1.00
0.50
1.00
1.00
1.00
0.20
4.00
Chemical name
Palmitic acid
PAMAM dendrimer
Paraformaldehyde
Pentachlorophenol (PCP
pesticide)
Peptone
Perchloric acid
Periodic acid
Peroxidase ( Lactoperoxidase )
Petroleum spirit (ligroin)
Phenol
Phenol red BDH 20090
Phenol SAT. Citrate Buffer
Phenol:Chlororform:
Isoamylalcohol (25:24:1)
Manufacturer
Product Code
CAS Number
P 0500
57-10-3
Sigma
412449
163442-67-9
Sigma
P 6148
30525-89-4
Sigma
P 2604
87-86-5
70171
73049-73-7
10176 244252
7601-90-3
BDH
P5463
10450-60-9
Sigma
L 2005
9003-99-0
333417
8032-32-4
BDH
P 5566
108-95-2
BDH
P 3532
143-74-8
sigma
P 4682
108-95-2
P 3803
136112-00-0
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
3.00
8.00
4.00
0.05
2.00
1.00
0.50
MIX
Sigma
Sigma
1.60
8.00
1.00
8.00
12.00
Chemical name
Phenolphthalein
Phenolphthalein diphosphate
sodium salt BDH 40037 3E
Phenylmethyl sulfonyl flouride
Phosphate buffered saline (PBS)
tablets
Phospholipase D
Phosphorous pentoxide
Phosphotungstic acid soln
Piperidine
PIPES
Plasminogen
Poly(isobutylmethacrylate) high
MR
Product Code
CAS Number
P 9750
77-09-8
BDH
P 9875
68807-90-9
sigma
P 7626
329-98-6
Sigma
BR14
MIXTURE
Oxoid
P8023
9001-87-0
Sigma
79609 298220
1314-56-3
Fluka
HT152
12501-23-4
Sigma
PC737-0586 FP356
110-89-4
Biotechnology Systems
P 9291 or P 1851 5625-37-6
Sigma
P9156
9001-91-6
Sigma
181544
9011-15-8
Aldrich
Polyanetholsulfonic acid sod. salt
Polyethylene Glycol 4,000 BDH
44273
Manufacturer
25322-68-3
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
0.50
2.00
0.50
0.10
3.00
2.00
2.00
0.50
0.50
0.25
2.00
52993-95-0
P 4338
Hazard
pictogram
sigma
0.50
Chemical name
Manufacturer
Product Code
CAS Number
81271
1405-20-5
Sigma
P 8136
9002-89-5
Sigma
P 5288
9003-39-8
Sigma
P7170
6226-79-5 mix
Sigma
Potassium Acetate BDH
103504X
P 5708
127-08-2
BDH
Potassium antimony tartrate
hydrate
230057
331753-56-1
Sigma
P 9144
298-14-6
M&B
209619
584-08-7
BDH
P 3911
7447-40-7
sigma
P 5379
7778-77-0
sigma
P2588
7778-50-9
BDH
Potassium ferricyanide
(potassium hexacyanoferrate
P 8131
13746-66-2
Potassium ferrocyanide
(potassium hexacyanoferrate (II)
455989 P 9387
14459-95-1
Polymyxin B sulfate
Polyvinyl alcohol
Polyvinylpyrrolidone(pvp)
Ponceau
Potassium bicarbonate
Potassium carbonate 10196
Potassium chloride BDH 29594
Potassium di-hydrogen
orthophosphate BDH 10203
Potassium dichromate
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.50
0.50
4.50
0.50
2.00
0.50
1.00
0.50
0.50
8.00
2.00
BDH
2.00
Chemical name
Potassium hydroxide BDH 10210
Potassium iodide BDH 29631
Potassium Nitrate
Potassium permanganate
Potassium persulphate
Potassium sodium tartrate
Potassium sulfate
Potassium tellurite BDH 29663
Potassium tetraborate dibasic
tetrahydrate
Potassium thiocynate
Propidium iodide
Propionic Acid
Protease
Manufacturer
Product Code
CAS Number
P 1767
1310-58-3
sigma
7681-11-0
Sigma
P 6162
7757-79-1
sigma
P 2097 (60467)
7722-64-7
Sigma
379824
7727-21-1
BDH
S 6170
6381-59-5
sigma
P 0772
7778-80-5
Sigma
P 0677
123333-66-4
sigma
689800 P5754
12045-78-2
Hopkin&Williams
P 2713
333-20-0
P 4170
25535-16-4
sigma
P-7560-PB17
79-09-4
Fisher
P5147
9036-06-0
Sigma
P 2963
60399
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
0.50
2.00
2.00
4.00
0.50
0.50
2.00
1.00
4.00
1.00
2.00
1.60
Chemical name
Proteinase K
A3830,0100
Applichem
Product Code
CAS Number
P 2308
39450-01-6
Putrescine dihydrochloride
Pyridine
Pyridoxal HCl
Raffinose Thomas Kerfoot
LT49868
Resazurin
Resorcinol
Riboflavin
Ribonuclease A
Rifampicin
Rubidium chloride
Ruthenium red
Safranin
Manufacturer
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
333-93-7
9728 360570
110-86-1
Merck
P 9130
65-22-5
Sigma
R 0514
17629-30-0
Sigma
199303
62758-13-8
Sigma
30019; 0119002
108-46-3
BDH; AnalaR
R 4500
83-88-5
Sigma
R4875; 109142
9001-99-4
Sigma; Boehringer
Mannheim
R 3501
13292-46-1
Sigma
83979
7791-11-9
Fluka
R2751
11103-72-3
Sigma
94635
MIXTURE
Biochemica
2.00
0.50
0.50
1.00
3.00
0.50
0.20
2.00
0.50
0.50
1.00
Chemical name
Salicylic Acid BDH 10230
Saline tablets (sodium chloride)
Sand, white quartz
Sarkosyl
Selenium (IV) oxide
Sephacryl 300
Sephacryl S200
Sephadex
Sephadex G200
Shikimic Acid
Sigmamarker wide range
Silane
Silica gel
Manufacturer
Product Code
CAS Number
S 5922
69-72-7
sigma
Br53 S7653
7647-14-5
Oxoid
274739
14808-60-7
Aldrich
44275 3R 61739
97-78-9
BDH
200107
7446-08-4
Sigma
S300HR
65546-95-4 mix
sigma
S200HR
65546-95-4
Pharmacia
spc25120
61840-62-8
Sigma
S 5897
9048-71-9
Pharmacia
S 5375
138-59-0
Sigma
M4038
MIXTURE
Sigma
333891
7803-62-5
BDH
420883
MIXTURE
BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
0.10
1.00
1.00
2.00
2.00
2.00
0.50
0.50
0.50
8.00
8.00
1.00
Chemical name
Manufacturer
Product Code
CAS Number
S 7625
112926-00-8
sigma
22 116 3
20667-12-3
Aldrich
Silver Nitrate Johnson Matthey
50001
S 8157
7761-88-8
sigma
Silver paint (Silver in
methylisobutylketone)
293261
108-10-1
Agar scientific
204412
10294-26-5
Riedel de Haen
176435
2923-28-6
Aldrich
S7400
7784-46-5
Sigma
S1878; S1147
7790-28-5
Sigma
S 2889
127-09-3
Sigma
S 2404
none
S 7670
6131-90-4
BDH
30111
26628-22-8
Sigma
B 3420
532-32-1
Silica Gel BDH BDH 30060
Silver (1) oxide
Silver sulphate
Silver trifluoromethane-sulfonate
Sodium (meta) arsenite
Sodium (meta) periodate
Sodium Acetate BDH 301045M
Sodium acetate buffer solution
Sodium acetate trihydrate
Sodium Azide
Sodium benzoate
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
1.00
2.00
1.00
1.00
1.00
3.00
2.00
0.50
0.50
0.50
4.00
0.50
Chemical name
Manufacturer
Product Code
CAS Number
S 9640
1303-96-4
Sigma
205591
15681-89-7
Merck
S9125; 30114
16940-66-2
Sigma; BDH
C 0250
6131-99-3
Sigma
S 7795
497-19-8
sigma
S/2700/63
6132-02-1
Fisher
S 7653
7647-14-5
sigma
S8628
25895-60-7
Sigma
Sodium deoxycholate Hopkin &
Williams 783400
D 6750
302-95-4
sigma
Sodium dichloroisocyanurate
dihydrate
35915-250G
51580-86-0
Sigma
Sodium dihydrogen
orthophosphate anhydrous BDH
S 8282
7558-80-7
sigma
Sodium dihydrogen
orthophosphate BDH 10245
S 9638
10049-21-5
sigma
Sodium dihydrogen
orthophosphate dihydrate
301324
13472-35-0
BDH
Sodium borate S-9640
Sodium borodeuteride
Sodium borohydride
Sodium cacodylate trihydrate
Sodium Carbonate BDH
102404H
Sodium carbonate decahydrate
Sodium chloride BDH
Merck
Sodium cyanoborohydride
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
8.00
4.00
2.00
1.00
1.00
0.10
3.00
1.00
3.00
0.50
0.50
0.50
Chemical name
Manufacturer
Product Code
CAS Number
L 1375
72-17-3
Sigma
456020
141-53-7
BDH
G7132
338950-81-5
HA1M
9067-32-7
Lifecore
Sodium Hydrogen Carbonate
BDH 10247
S 6297
144-55-8
BDH
Sodium hydroxide BDH 10252
5P
S 8045 261846P
1310-73-2
sigma
239305
7681-52-9
Sigma
L 7022
867-56-1
63260
371-47-1
BDH
243655
7631-95-0
Sigma
S 6646
10102-40-6
sigma
S 5506
7631-99-4
sigma
431605
7632-00-0
Hop wil
Sodium DL-lactate
Sodium formate
Sodium glycocholate hydrate
Sodium hyaluronate
Sodium hypochlorite
Sodium L-lactate
Sodium maleate
Sodium Molybdate
Sodium Molybdate 2H2O BDH
102542Q
Sodium nitrate BDH 30187 4S
Sodium nitrite
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
0.50
0.50
0.50
3.00
3.00
0.50
1.00
1.00
1.00
2.00
3.00
Chemical name
Sodium oxalate 102584
Sodium percarbonate
Sodium phosphate
Sodium phosphate
Sodium phosphate di basic
Sodium phosphate dibasic
heptahydrate
Sodium propionate
Sodium pyrophosphate
Sodium pyruvate
Sodium salicylate
Sodium selenite
Sodium selenite pentahydrate
Sodium sticks in paraffin
Manufacturer
Product Code
CAS Number
O 0136
62-76-0
BDH
371432
15630-89-4
Aldrich
431613
10101-89-0
Sigma
342483
7601-54-9
Sigma
S 9763
7558-79-4
Sigma
S9390
7782-85-6
sigma
P 1880
137-40-6
Sigma
221368
13472-36-1
BDH
P 8574
113-24-6
28065.230
54-21-7
BDH
S 9133
10102-18-8
BDH
S 9133
26970-82-1
BDH
30101 3E 13401
7440-23-5
BDH
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
3.00
1.00
4.00
0.50
0.50
1.00
1.00
0.50
2.00
4.00
4.00
2.00
Chemical name
Sodium succinate BDH 30219
Sodium Sulphate BDH 30223
Sodium sulphide
Sodium sulphite BDH 30226
Sodium tartrate
Sodium tetraborate ( Borax )
Sodium thiosulphate 5H2O BDH
30235 5E
Sodium thiosulphate anhydrous
Sodium tungstate
Sorbic acid (potassium salt)
Sorbitol
Soybean oil
Sparfloxacin
Manufacturer
Product Code
CAS Number
S 2378
6106-21-4
BDH
S 6264
7757-82-6
sigma
S 2006
1313-84-4
Sigma
S 8018
7757-83-7
sigma
S 4797
6106-24-7
BDH
221732
1330-43-4
sigma
S 7143
10102-17-7
sigma
S725053
7772-98-7
Fisher
T 2629
10213-10-2
Sigma
S 1751
24634-61-5
S 0900
50-70-4
S 7381
8001-22-7
56968
110871-86-8
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
1.00
0.50
3.00
2.00
0.50
4.00
0.50
0.50
1.00
1.00
Sigma
0.50
0.05
1.00
Chemical name
Spectinomycin
Spectinomycin
Spermidine
Sphingomyelin
ß-Lactose
Starch BDH 10271 Analar
Staurosporin
Streptomycin Sulphate
Succinic acid
Succinic semialdehyde 15% soln
Sulfamic acid
Sulphanilamide
Sulphanilic acid BDH 10275 2B
Manufacturer
Product Code
CAS Number
S 9007
22189-32-8
Sigma
S 9007
22189-32-8
Sigma
S 0266
124-20-9
Sigma
S7004
85187-10-6
Sigma
L 8773
5965-66-2
Sigma
S 5651
9005-25-8
sigma
S 4400
62996-74-1 +
S 6501
3810-74-0
Sigma
83674
110-15-6
sigma
S 1505
692-29-5
Sigma
481505
5329-14-6
Sigma
1.11799.0100
63-74-1
Millipore
S 5263
121-57-3
sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.50
0.50
1.00
0.10
0.50
0.50
4.00
2.00
2.00
1.00
2.00
0.50
1.00
Chemical name
Manufacturer
Product Code
CAS Number
320501
7664-93-9
Aldrich
S2671
129-46-4
Sigma
T 8691
107-35-7
Sigma
Taurocholic acid sodium salt
hydrate
86339
345909-26-4
Sigma
TEMED NNNN
(Tetramethylethylenediamine)
T 9281
110-18-9
Sigma
19460, B 2138
75-65-0
Sigma
T 3258
60-54-8
Sigma
T 8032
64-75-5
T 4625
67-03-8
M 2128
298-93-1
T5125
54-64-8
Sigma
T 0895
65-71-4
Sigma
T4014
32986-56-4
Sigma
Sulphuric Acid
Suramin
Taurine
tert-butanol
Tetracycline
Tetracycline HCl
Thiamine Vit B1(aneurineHCl)
Thiazolyl blue Tetrazolium
Bromide
Thimerosal
Thymine
Tobramycin
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
0.25
1.00
1.00
2.00
2.00
2.00
1.00
Sigma
0.50
0.50
4.00
0.50
0.50
Chemical name
Manufacturer
Product Code
CAS Number
102846G 244511
108-88-3
BDH
T 3260
92-31-9
sigma
T 9531
6138-23-4
sigma
85-4, S 5770,
MIXTURE
Sigma Fluka
342483
7601-54-9
Sigma
tri-Sodium citrate BDH
102425M
C 3434
6132-04-3
sigma
Tri(235)phenyltetrazolium chloride
BDH 34072 5W
T 8877
298-96-4
sigma
W200700
102-76-1
sigma
T 4885
76-03-9
T 9784
5704-04-1
Toluene
Toluidine blue Sigma T 3260 ci
52040
Trehalose BDH 38063
Tri sodium citrate solution
Tri sodium orthophosphate 30193
Triacetin
Trichloroacetic acid
Tricine
Triethylamine
Trifluoroacetic Acid BDH 30508
Trimethoprim
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
4.00
0.50
0.05
0.50
2.00
0.50
1.00
0.50
3.00
0.50
121-44-8
Sigma
T 6508
76-05-1
sigma
T 7883
738-70-5
3.00
4.00
0.50
Chemical name
Trimethylamine
Tris EDTA Buffer
Triton X-100
Triton X-114
Trizma base (Tris Base)
(trishydroxymethylaminomethane)
Trizma Hydrochloride
Trypan blue
Trypsin
Tween 20
Tween 80
Uracil
Urea BDH 10290 4W
Valeric acid
Manufacturer
Product Code
CAS Number
243205
75-50-3
Sigma
T9285
T9285
Sigma
X100
9002-93-1
Sigma
X114
9036-19-5
Sigma
T 1503
77-86-1
Sigma
T 3253
1185-53-1
Sigma
T0776 T6146
72-57-1
Sigma
T6424
9002-07-7
Sigma
P1379
9005-64-5
Aldrich
P1754
9005-65-6
Sigma
U 0750
66-22-8
Sigma
U 6504
57-13-6
Sigma
V 9759
109-52-4
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
2.00
0.50
2.00
2.00
1.00
1.00
4.00
0.80
0.50
0.50
0.50
0.50
2.00
Chemical name
Visigel contains propanol
Vitamin B12
X-gal Roche 1680293
Xanthine
Xanthine oxidase from bovine
milk
Xylene
Xylene cyanol
Zinc acetate
Zinc Chloride BDH 436982Q
Zinc sulphate BDH 10296
β-Cyclodextrin
β-Glycerol phosphate disodium
salt pentahydrate
Manufacturer
Product Code
CAS Number
I 9516
67-63-0
Sigma
V 2876
68-19-9
Sigma
B 4252
7240-90-6
sigma
X7375
69-89-6
Sigma
X4500
9002-17-9
Sigma
294780
95-47-6
BDH
335940
2650-17-1
sigma
Z 0625
5970-45-6
BDH
Z 3500
7646-85-7
sigma
Z 4750
7446-20-0
sigma
C4767
7585-39-9
Sigma
50020
13408-09-8
Sigma
Hazard
pictogram
Hazard
Hazard
Hazard
pictogram 2 pictogram 3 pictogram 4
Hazard
Rating
0.80
0.50
0.50
4.00
1.00
2.00
1.00
1.00
2.00
2.00
0.25
0.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
2 methoxy ethanol
284467
sigma
CAS Number
109-86-4
Storage
Room Number
Risk Factors
MSDS PDF LINK
109-86-4.pdf
__ Date: 29-05-2013
Amount
1000ml
R10 Flammable.
R20 Harmful by inhalation.
R21 Harmful in contact with skin.
R22 Harmful if swallowed.
R60 May impair fertility.
R61 May cause harm to the unborn child.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
H226: Flammable liquid and vapour
H302: Harmful if swallowed
H312: Harmful in contact with skin
H332: Harmful if inhaled
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Acrylamide
A 9099
BDH
Room Number
Risk Factors
MSDS PDF LINK
79-06-1.pdf
__ Date: 29-05-2013
CAS Number
79-06-1
Storage
Amount
1000g
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R23 Toxic by inhalation. R24 Toxic in contact with skin.
R25 Toxic if swallowed. R36 Irritating to eyes.
R38 Irritating to skin. R43 May cause sensitization by skin contact.
R45 May cause cancer. R46 May cause heritable genetic damage.
R48 Danger of serious damage to health by prolonged exposure.
R62 Risk of impaired fertility.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed. H312: Harmful in contact with skin
H315: Causes skin irritation. H317: May cause an allergic skin reaction
H319: Causes serious eye irritation. H332: Harmful if inhaled
H340: May cause genetic defects. H350: May cause cancer
H361: Suspected of damaging fertility or the unborn child
H372: Causes damage to organs through prolonged or repeated exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional usera only. PREGNANCY ALERT
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Acrylamide bis
A 2917
Bio rad
Room Number
Risk Factors
MSDS PDF LINK
Acrylbisacryl.pd
__ Date: 29-05-2013
CAS Number
MIXTURE
acrylbisacryl
Storage
Amount
500ml
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation. R24 Toxic in contact
with skin. R25 Toxic if swallowed. R36 Irritating to eyes.
R38 Irritating to skin. R43 May cause sensitization by skin contact.
R45 May cause cancer. R46 May cause heritable genetic damage.
R48 Danger of serious damage to health by prolonged exposure.
R62 Risk of impaired fertility.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed •
H312: Harmful in contact with skin
•
H315: Causes skin irritation • H317: May cause an allergic skin
reaction•
H319: Causes serious eye irritation • H332: Harmful if
inhaled
•
H340: May cause genetic defects •
H350: May cause cancer
•
H361: Suspected of damaging fertility or the unborn child
•
H372: Causes damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Group B Harmful R 20, 21, 22, 33,40, 67
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional users only. PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Acrylamide/bisacrylamide
A 2917
Biorad
Room Number
Risk Factors
MSDS PDF LINK
Acrylbisacryl.pd
__ Date: 29-05-2013
CAS Number
MIXTURE
Storage
Amount
500ml
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation. R24 Toxic in contact
with skin. R25 Toxic if swallowed. R36 Irritating to eyes.
R38 Irritating to skin. R43 May cause sensitization by skin contact.
R45 May cause cancer. R46 May cause heritable genetic damage.
R48 Danger of serious damage to health by prolonged exposure.
R62 Risk of impaired fertility.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed •
H312: Harmful in contact with skin
•
H315: Causes skin irritation • H317: May cause an allergic skin
reaction•
H319: Causes serious eye irritation • H332: Harmful if
inhaled
•
H340: May cause genetic defects •
H350: May cause cancer
•
H361: Suspected of damaging fertility or the unborn child
•
H372: Causes damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Group B Harmful R 20, 21, 22, 33,40, 67
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional users only. PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Acrylamide/bisacrylamide
A 9926
Biorad
Room Number
Risk Factors
MSDS PDF LINK
Acrylbisacryl.pd
__ Date: 29-05-2013
CAS Number
MIXTURE
Storage
Amount
500ml
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation. R24 Toxic in contact
with skin. R25 Toxic if swallowed. R36 Irritating to eyes.
R38 Irritating to skin. R43 May cause sensitization by skin contact.
R45 May cause cancer. R46 May cause heritable genetic damage.
R48 Danger of serious damage to health by prolonged exposure.
R62 Risk of impaired fertility.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed •
H312: Harmful in contact with skin
•
H315: Causes skin irritation • H317: May cause an allergic skin
reaction•
H319: Causes serious eye irritation • H332: Harmful if
inhaled
•
H340: May cause genetic defects •
H350: May cause cancer
•
H361: Suspected of damaging fertility or the unborn child
•
H372: Causes damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Group B Harmful R 20, 21, 22, 33,40, 67
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional users only. PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Acrylamide/bisacrylamide soln 37:5:1
A 2917
Bio rad
Room Number
Risk Factors
MSDS PDF LINK
Acrylbisacryl.pd
__ Date: 29-05-2013
CAS Number
MIXTURE
Storage
Amount
500ml
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation. R24 Toxic in contact
with skin. R25 Toxic if swallowed. R36 Irritating to eyes.
R38 Irritating to skin. R43 May cause sensitization by skin contact.
R45 May cause cancer. R46 May cause heritable genetic damage.
R48 Danger of serious damage to health by prolonged exposure.
R62 Risk of impaired fertility.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed •
H312: Harmful in contact with skin
•
H315: Causes skin irritation • H317: May cause an allergic skin
reaction•
H319: Causes serious eye irritation • H332: Harmful if
inhaled
•
H340: May cause genetic defects •
H350: May cause cancer
•
H361: Suspected of damaging fertility or the unborn child
•
H372: Causes damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Group B Harmful R 20, 21, 22, 33,40, 67
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional users only. PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Aniline
10041 4B 242284
BDH
Room Number
Risk Factors
MSDS PDF LINK
62-53-3.pdf
__ Date: 29-05-2013
62-53-3
Storage
Chemical cabinet
500ml
Amount
CAS Number
R23 Toxic by inhalation.R24 Toxic in contact with skin.
R25 Toxic if swallowed. R40 Possible risk of irreversible effects.
R41 Risk of serious damage to the eyes.
R43 May cause sensitization by skin contact.
R48 Danger of serious damage to health by prolonged exposure.
R50 Very toxic to aquatic organisms.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H372: Causes damage to organs through prolonged or repeated
exposure
•
H400: Very toxic to aquatic life
•
H301: Toxic if swallowed
H311: Toxic in contact with skin
•
H317: May cause an allergic skin reaction
•
H318: Causes serious eye damage
H331: Toxic if inhaled
•
H341: Suspected of causing genetic defects
•
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice. S27 Take off immediately all contaminated
clothing. S36 Wear suitable protective clothing. S37 Wear suitable gloves.
S39 Wear eye / face protection. S45 In case of accident or if you feel
unwell, seek medical advice immediately (show the label whenever
possible.) S46 If swallowed, seek medical advice immediately and show
this container or label. S61 Avoid release to the environment. Refer to
special instructions / safety data sheets. S63 In case of accident by
inhalation, remove casualty to fresh air and keep at rest
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
P273: Avoid release to the environment P280: Wear protective
gloves/protective clothing/eye protection/face protection P301+310: IF
SWALLOWED: Immediately call a POISON CENTER or doctor/physician
P305+351+338: IF IN EYES: Rinse continuously with water for several
minutes. Remove contact lenses if present and easy to do – continue
rinsing P311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
2
1
1
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Arsenic acid potassium salt (Potassium arsenate)
CAS Number
7784-41-0
A 6631
Storage
Sigma
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
50g
R23 Toxic by inhalation.
R25 Toxic if swallowed.
R45 May cause cancer.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
7784-41-0.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
H301: Toxic if swallowed
H331: Toxic if inhaled
H350: May cause cancer
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Autofluor
Product Code
LS315
Nat Diag
Manufacturer
CAS Number
__ Date: 29-05-2013
NONE
Storage
Room Number
Risk Factors
MSDS PDF LINK
Amount
1000ml
R36 Irritating to eyes.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R22 Harmful if swallowed.
R46 May cause heritable genetic damage.
Autoflour.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation
H302: Harmful if swallowed
H340: May cause genetic defects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S24 Avoid contact with skin.
S25 Avoid contact with eyes.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S28 After contact with skin, wash immediately with plenty of soap-suds.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Boric Acid (Orthoboric Acid )
B 7660
Sigma
CAS Number
10043-35-3
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R60 May impair fertility.
R61 May cause harm to the unborn child.
10043-35-3.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Carbol fuchsin solution
21820
-
mixture
Storage
Shelf
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
100ml
R20 Harmful by inhalation.
R21 Harmful in contact with skin.
R22 Harmful if swallowed.
R34 Causes burns.
R68 Possible risk of irreversible effects.
arbol fuchsin.pd
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22
Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
5
0.2
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Cefotaxime
C 7912
sigma
CAS Number
64485-93-4
Storage
Room Number
Risk Factors
MSDS PDF LINK
Amount
1g
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
64485-93-4.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H317: May cause an allergic skin reaction
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S22 Do not breathe dust.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P342+311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Cephradine
C 8395
sigma
CAS Number
38821-53-3
Storage
Room Number
Risk Factors
MSDS PDF LINK
Amount
500 mg
R36 Irritating to eyes.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
38821-53-3.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H315: Causes skin irritation
•
H317: May cause an allergic skin reaction
•
H319: Causes serious eye irritation
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
•
H335: May cause respiratory irritation
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P342+311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group A Irritant R36, 38,
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
OTHER HAZARD CLASS Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Chloramphenicol
C 7795
Sigma
CAS Number
56-75-7
Storage
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
25
R45 May cause cancer.
MSDS PDF LINK
56-75-7.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Chloroform BDH 10077
C 2432
sigma
67-66-3
Storage
solvent cabinet
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R20 Harmful by inhalation.
R22 Harmful if swallowed.
R38 Irritating to skin.
R40 Possible risk of irreversible effects.
R48 Danger of serious damage to health by prolonged exposure.
67-66-3.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
•
H315: Causes skin irritation
•
H351: Suspected of causing cancer
•
H373: May cause damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P281: Use personal protective equipment as required
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Contains trace of 2 methyl butene see MSDS 513-35-9
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sectionsin MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Cobalt (11) nitrate hexahydrate
Product Code
Manufacturer
CAS Number
Sigma
Room Number
Risk Factors
MSDS PDF LINK
10026-22-9.pdf
__ Date: 29-05-2013
10026-22-9
Storage
Amount
R8 Contact with combustible material may cause fire.
R22 Harmful if swallowed.
R40 Possible risk of irreversible effects.
R43 May cause sensitization by skin contact.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H272: May intensify fire; oxidizer •
H302: Harmful if swallowed
•
H317: May cause an allergic skin reaction • H334: May cause
allergy or asthma symptoms of breathing difficulties if inhaled •
H341: Suspected of causing genetic defects •
H350: May cause
cancer
•
H360: May damage fertility or the unborn child
•
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S17 Keep away from combustible material.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P220: Keep/Store away from clothing/…/combustible materials
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
__ Date: 29-05-2013
Chemical Hazard Group
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sectionsin MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
__ Date: 29-05-2013
Manufacturer
Cobalt (II) Chloride 6-hydrate BDH 277902M
CAS Number
7791-13-1
C 2644
Storage
sigma
Small Bottles
Room Number
Amount
Chemical name
Product Code
Risk Factors
R49 May cause cancer by inhalation.
R60 May impair fertility.
R22 Harmful if swallowed.
R68 Possible risk of irreversible effects.
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
7791-13-1.pdf
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
•
H317: May cause an allergic skin reaction
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
•
H410: Very toxic to aquatic life with long lasting effects
•
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P308+313: IF exposed or concerned: Get medical advice/attention
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group B Harmful R 20, 21, 22,
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sectionsin MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
Cobalt sulfate
229598
Sigma
CAS Number
60459-08-7
Storage
Amount
100g
Risk Factors
R22 Harmful if swallowed.
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
R49 May cause cancer by inhalation.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
60459-08-7.pdf
R60 May impair fertility.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
•
H317: May cause an allergic skin reaction
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
•
H410: Very toxic to aquatic life with long lasting effects
•
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S22 Do not breathe dust.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P308+313: IF exposed or concerned: Get medical advice/attention
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
Cobaltous chloride
449776
BDH
CAS Number
7646-79-9
Storage
Amount
100g
Risk Factors
R22 Harmful if swallowed.
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
R49 May cause cancer by inhalation.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
7646-79-9.pdf
R60 May impair fertility.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
•
H317: May cause an allergic skin reaction
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
•
H410: Very toxic to aquatic life with long lasting effects
•
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P308+313: IF exposed or concerned: Get medical advice/attention
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Dimethylformamide BDH 10322
D 4551
Sigma
68-12-2
Storage
Fume Hood
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
500ml
R61 May cause harm to the unborn child.
R36 Irritating to eyes.
R20 Harmful by inhalation.
R21 Harmful in contact with skin.
68-12-2.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
H226: Flammable liquid and vapour
H312: Harmful in contact with skin
H319: Causes serious eye irritation
H332: Harmful if inhaled
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P201: Obtain special instructions before use
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Diphenylamine
10101 D 2385
BDH
Room Number
Risk Factors
MSDS PDF LINK
122-39-4.pdf
__ Date: 29-05-2013
122-39-4
Storage
Shelf
CAS Number
Amount
100g
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R33 Danger of cumulative effects.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed
•
H311: Toxic in contact with skin
•
H331: Toxic if inhaled
•
H373: May cause damage to organs through prolonged or repeated
exposure
•
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S28 After contact with skin, wash immediately with plenty of soap-suds.
S36 Wear suitable protective clothing. S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment. P280: Wear protective
gloves/protective clothing/eye protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1.5
1
4
6.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Ethanol MERCK 100983-2500
E 7023
sigma
CAS Number
64-17-5pure
Storage
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
R11 Highly flammable.
MSDS PDF LINK
64-17-5pure.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H225: Highly flammable liquid and vapour
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
S16 Keep away from sources of ignition.
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Flammable
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
2.5
2
2
10.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Foam or CO2 Extinguisher
Naked Flame,
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Ethidium Bromide
E 7637
Sigma
CAS Number
1239-45-8
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R26 Very toxic by inhalation.
R68 Possible risk of irreversible effects.
1239-45-8.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
H302: Harmful if swallowed
H330: Fatal if inhaled
H341: Suspected of causing genetic defects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S28 After contact with skin, wash immediately with plenty of soap-suds.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S63 In case of accident by inhalation, remove casualty to fresh air and
keep at rest
•
•
•
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
P281: Use personal protective equipment as required
P284: Wear respiratory protection
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
OTHER HAZARD CLASS Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read MSDS
Handling Electrophoresis gels
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Ethidium Bromide Tablets
E 4391
Sigma
1239-45-8
Storage
Fume Hood
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R26 Very toxic by inhalation.
R68 Possible risk of irreversible effects.
1239-45-8.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
H302: Harmful if swallowed
H330: Fatal if inhaled
H341: Suspected of causing genetic defects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S28 After contact with skin, wash immediately with plenty of soap-suds.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S63 In case of accident by inhalation, remove casualty to fresh air and
keep at rest
•
•
•
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
P281: Use personal protective equipment as required
P284: Wear respiratory protection
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
OTHER HAZARD CLASS Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read MSDS
Handling Electrophoresis gels
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Fast Blue B salt
D 9805
Sigma
CAS Number
MSDS PDF LINK
14263-94-6 + mixture
Storage
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
10g
R45 May cause cancer.
R22 Harmful if swallowed.
14263-94-6.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
H302: Harmful if swallowed
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group B Harmful R 20, 21, 22,
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Formaldehyde BDH 10113
F 8775
sigma
Room Number
Risk Factors
MSDS PDF LINK
50-00-0.pdf
__ Date: 29-05-2013
CAS Number
50-00-0
Storage
Amount
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R34 Causes burns.
R39 Danger of very serious irreversible effects.
R40 Possible risk of irreversible effects.
R43 May cause sensitization by skin contact.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
H301: Toxic if swallowed
H311: Toxic in contact with skin
H314: Causes severe skin burns and eye damage
H317: May cause an allergic skin reaction
H331: Toxic if inhaled
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing. S37 Wear suitable gloves.
S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S51 Use only in well ventilated areas.
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection. P301+310: IF SWALLOWED: Immediately call a
POISON CENTER or doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted To Trained Professional Only. PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Formamide BDH 28422
F 4761
sigma
75-12-7
Storage
Fume Hood
CAS Number
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
R61 May cause harm to the unborn child.
MSDS PDF LINK
75-12-7.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Fuchsin basic Gurr BDH 34032
47860
sigma fluka
58969-01-0
Storage
cabinet
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R40 Possible risk of irreversible effects.
58969-01-0.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
•
H317: May cause an allergic skin reaction
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled
•
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
S28 After contact with skin, wash immediately with plenty of soap-suds.
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P342+311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
5
0.2
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Glycidyltrimethylammonium chloride
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
CAS Number
Sigma
3033-77-0
Storage
Amount
Risk Factors
R45 May cause cancer. R60 May impair fertility. R20 Harmful by
inhalation.
R21 Harmful in contact with skin. R22 Harmful if swallowed.
R48 Danger of serious damage to health by prolonged exposure.
R68 Possible risk of irreversible effects. R41 Risk of serious damage to
the eyes. R43 May cause sensitization by skin contact.
3033-77-0.pdf
R52 Harmful to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed
•
H312: Harmful in contact with skin H317 • May cause an allergic
skin reaction
•
H318: Causes serious eye damage • H341: Suspected of causing
genetic defects • H350: May cause cancer
•
H360: May damage fertility or the unborn child
•
H373: May cause damage to organs through prolonged or repeated
exposure • H412: Harmful to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice. S36 Wear suitable protective clothing.
S37 Wear suitable gloves. S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.) S61 Avoid release to the
environment. Refer to special instructions / safety data sheets.
•
P201: Obtain special instructions before use •
P273: Avoid
release to the environment •
P280: Wear protective gloves/protective
clothing/eye protection/face protection •
P301+310: IF SWALLOWED:
Immediately call a POISON CENTER or doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
Group E Sensitisers & Carcinogens R 42, 45, 46, 49, 68
OTHER HAZARD CLASS Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Hydrazine
216046
Sigma
Room Number
Risk Factors
MSDS PDF LINK
10034-93-2.pdf
__ Date: 29-05-2013
CAS Number
10034-93-2
Storage
Amount
100g
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R43 May cause sensitization by skin contact.
R45 May cause cancer.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
•
H302: Harmful if swallowed
H311: Toxic in contact with skin
H314: Causes severe skin burns and eye damage
H317: May cause an allergic skin reaction
H330: Fatal if inhaled
H350: May cause cancer
H400: Very toxic to aquatic life
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
P201: Obtain special instructions before use
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P284: Wear respiratory protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Hydroquinone
H 9003
Sigma
CAS Number
123-31-9
Storage
Room Number
Risk Factors
MSDS PDF LINK
123-31-9.pdf
Amount
500g
R22 Harmful if swallowed.
R40 Possible risk of irreversible effects.
R41 Risk of serious damage to the eyes.
R43 May cause sensitization by skin contact.
R50 Very toxic to aquatic organisms.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
H400: Very toxic to aquatic life
H302: Harmful if swallowed
H317: May cause an allergic skin reaction
H318: Causes serious eye damage
H341: Suspected of causing genetic defects
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S39 Wear eye / face protection.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Imidazol
I 5513
Sigma; Aldrich
MSDS PDF LINK
288-32-4
Storage
Shelf
CAS Number
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
250g
R22 Harmful if swallowed
R34 Causes burns
288-32-4.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
H301: Toxic if swallowed
H304: May be fatal if swallowed and enters airways
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S36 Wear suitable protective clothing
S37 Wear suitable gloves
S39 Wear eye / face protection
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36, 37, 38, 39, 41, 48
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
2
1.5
1
2
6.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Industrial Methylated Spirit (Bench Alcohol)
CAS Number
64-17-5IMS
458600
Manufacturer
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
2500ml
R11 Highly flammable.
R20 Harmful by inhalation.
R21 Harmful in contact with skin.
R22 Harmful if swallowed.
R68 Possible risk of irreversible effects.
64-17-5IMS.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
H225: Highly flammable liquid and vapour
H302: Harmful if swallowed
H371: May cause damage to organs
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S16 Keep away from sources of ignition.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
EXTRA CARE HAS TO BE OBSERVED WHEN USING IMS FOR FLAME
STERILISING PLATE SPREADERS. STAINLESS STEEL DISHES AND
SPREADERS ARE ROUTINELY USED.
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36, 37, 38, 39, 41, 48
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
3
1
1
4
12.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Undergraduates are trained in the use of lab. alcohol for sterilising
In the event of fire use Foam, Chemical powder or Carbon dioxide
Bunsens
Read Relevant Sections in MSDS contains 5-10% Methanol
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Iodomethane
101168123
Sigma
74-88-4
Storage
Shelf
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
74-88-4.pdf
Amount
100ml
R21 Harmful in contact with skin.
R23 Toxic by inhalation.
R25 Toxic if swallowed.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R40 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
H301: Toxic if swallowed
H312: Harmful in contact with skin
H315: Causes skin irritation
H331: Toxic if inhaled
H335: May cause respiratory irritation
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S38 In case of insufficient ventilation, wear suitable respiratory
equipment.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
HAZARD CLASS
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
3
3.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Iodomethane solution (in tert-butyl methyl ether cas1634-04-4)
CAS Number
74-88-4 mixture
67695
Storage
Fluka
Shelf
100ml
Amount
R11 Highly flammable.
R20 Harmful by inhalation.
R22 Harmful if swallowed.
R36 Irritating to eyes.
R40 Possible risk of irreversible effects.
4-88-4 mixture.p
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
H225: Highly flammable liquid and vapour
H302: Harmful if swallowed
H315: Causes skin irritation
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S16 Keep away from sources of ignition.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
3
3.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Kanamycin
K 4000
sigma
(Sulfate Boehringer 106 801)
CAS Number
25389-94-0
Storage
Fridge
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
R61 May cause harm to the unborn child.
MSDS PDF LINK
25389-94-0.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
Exercise appropriate precautions to minimize direct contact with skin or
eyes and prevent inhalation.
Avoid inhalation.
Avoid contact with eyes, skin, and clothing.
Avoid prolonged or repeated exposure.
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Kanamycin sulfate
60615
sigma
70560-51-9
Storage
Fridge
CAS Number
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
5g
R61 May cause harm to the unborn child.
MSDS PDF LINK
70560-51-9.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H360: May damage fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Lead acetate BDH 10143
L 3396
sigma
Room Number
Risk Factors
MSDS PDF LINK
6080-56-4.pdf
__ Date: 29-05-2013
6080-56-4
Storage
cabinet
CAS Number
Amount
R61 May cause harm to the unborn child.
R33 Danger of cumulative effects.
R62 Risk of impaired fertility.
R48 Danger of serious damage to health by prolonged exposure.
R22 Harmful if swallowed.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H360: May damage fertility or the unborn child
•
H373: May cause damage to organs through prolonged or repeated
exposure
•
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
•
•
•
plant
P201: Obtain special instructions before use
P273: Avoid release to the environment
P308+313: IF exposed or concerned: Get medical advice/attention
P501 Dispose of contents/container to an approved waste disposal
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
Group B Harmful R 20, 21, 22,
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Manganous Sulphate mono H2O BDH 10153
CAS Number
10034-96-5
M 7634
Storage
Sigma nearest 10034-96-5
Top Shelf
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R20 Harmful by inhalation.
R22 Harmful if swallowed.
R48 Danger of serious damage to health by prolonged exposure.
R51 Toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
10034-96-5.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H373: May cause damage to organs through prolonged or repeated
exposure
•
H411: Toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S22 Do not breathe dust.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P273: Avoid release to the environment
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 37, 39, 41, 43, 48
HAZARD CLASS
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
3
3.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Methanol
M 1775
Sigma
Room Number
Risk Factors
MSDS PDF LINK
67-56-1.pdf
__ Date: 29-05-2013
CAS Number
67-56-1
Storage
Amount
2500ml
Toxic
R11 Highly flammable.
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R39 Danger of very serious irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
H225: Highly flammable liquid and vapour
H301: Toxic if swallowed
H311: Toxic in contact with skin
H331: Toxic if inhaled
H370: Causes damage to organs
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S7 Keep container tightly closed.
S16 Keep away from sources of ignition.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P307+311: IF exposed: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36, 37, 38, 39, 41, 48
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
2.5
2
2
10.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
All users have undergone two years of laboratory practicals.
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Methyl violet BDH 34033 5M CI 42555
CAS Number
548-62-9
C 3886
Storage
sigma
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R40 Possible risk of irreversible effects.
R41 Risk of serious damage to the eyes.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
548-62-9.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
H302: Harmful if swallowed
H318: Causes serious eye damage
H351: Suspected of causing cancer
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice. S36 Wear suitable protective clothing.
S37 Wear suitable gloves. S39 Wear eye / face protection.
S46 If swallowed, seek medical advice immediately and show this
container or label. S60 This material and its container must be disposed of
as hazardous waste. S61 Avoid release to the environment. Refer to
special instructions / safety data sheets.
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P501 Dispose of contents/container to an approved waste disposal
plant
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
5
0.2
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sectionsin MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
n-Hexane
34859
Sigma
110-54-3
Storage
Cabinet
CAS Number
Amount
1.5 L
Risk Factors
R11 Highly flammable. R38 Irritating to skin.
R48 Danger of serious damage to health by prolonged exposure.
R20 Harmful by inhalation.
R51 Toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
R62 Risk of impaired fertility.
110-54-3.pdf
R65 Harmful: may cause lung damage if swallowed
R67 Vapours may cause drowsiness and dizziness
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H225: Highly flammable liquid and vapour
•
H315: Causes skin irritation
•
H304: May be fatal if swallowed and enters airways
•
H336: May cause drowsiness or dizziness
•
H373: May cause damage to organs through prolonged or repeated
exposure
•
H361: Suspected of damaging fertility or the unborn child
•
H411: Toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S9 Keep container in a well-ventilated place
S16 Keep away from sources of ignition S29 Do not empty into drains
S33 Take precautionary measures against static discharges
S36 Wear suitable protective clothing S37 Wear suitable gloves
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
S62 If swallowed, do not induce vomitting; seek medical advice
immediately and show this container or label.
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P281: Use personal protective equipment as required
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
N;N’-Disuccinimidyl carbonate
160928186
Sigma
CAS Number
74124-79-1
Storage
Room Number
Risk Factors
MSDS PDF LINK
Amount
R36 Irritating to eyes.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R22 Harmful if swallowed.
74124-79-1.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
H301: Toxic if swallowed
H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S37 Wear suitable gloves.
S39 Wear eye / face protection.
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group A Irritant R36, 38,
Group B Harmful R 20, 21, 22,
OTHER HAZARD CLASS Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36,37, 41, 43, 48
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
2
2.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Nickel (II) chloride hexahydrate
N 6136
Sigma
Room Number
Risk Factors
MSDS PDF LINK
7791-20-0.pdf
__ Date: 29-05-2013
CAS Number
7791-20-0
Storage
Amount
500g
R49 May cause cancer by inhalation. R61 May cause harm to the unborn
child. R23 Toxic by inhalation. R25 Toxic if swallowed. R48 Danger of
serious damage to health by prolonged exposure. R38 Irritating to skin.
R68 Possible risk of irreversible effects.R42 May cause sensitization by
inhalation. R43 May cause sensitization by skin contact.
R50 Very toxic to aquatic organisms. R53 May cause long-term adverse
effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed •
H315: Causes skin irritation
•
H317: May cause an allergic skin reaction • H331: Toxic if inhaled
•
H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled •
H341: Suspected of causing genetic defects
•
H350: May cause cancer •
H360: May damage fertility or the
unborn child •
H372: Causes damage to organs through prolonged or
repeated exposure •
H410: Very toxic to aquatic life with long lasting
effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Nitrilotriacetic Acid (Trisodium Salt)
N 0253
Sigma
5064-31-3
Storage
Top Shelf
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R40 Possible risk of irreversible effects.
5064-31-3.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
H302: Harmful if swallowed
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
S36 Wear suitable protective clothing.
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P281: Use personal protective equipment as required
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
PAMAM dendrimer
412449
Sigma
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
CAS Number
163442-67-9 mixture
Storage
Amount
R11 Highly flammable.
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R39 Danger of very serious irreversible effects.
PAMAM.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
H225: Highly flammable liquid and vapour
H301: Toxic if swallowed
H311: Toxic in contact with skin
H331: Toxic if inhaled
H370: Causes damage to organs
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S16 Keep away from sources of ignition.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Flammable
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36,37, 41, 43, 48
OTHER HAZARD CLASS Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40/21, 41, 43, 48, 66
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
3
3.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Paraformaldehyde
P 6148
Sigma
CAS Number
30525-89-4
Storage
Room Number
Amount
500g
Risk Factors
R11 Highly flammable.
R20 Harmful by inhalation.
R22 Harmful if swallowed.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R40 Possible risk of irreversible effects.
30525-89-4.pdf
R41 Risk of serious damage to the eyes.
R43 May cause sensitization by skin contact.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
•
•
H228: Flammable solid
H302: Harmful if swallowed
H315: Causes skin irritation
H317: May cause an allergic skin reaction
H318: Causes serious eye damage
H332: Harmful if inhaled
H335: May cause respiratory irritation
H351: Suspected of causing cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves
S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Group B Harmful R 20, 21, 22, 33,40, 67
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Two years of laboratory practicals. PREGNANCY ALERT
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Pentachlorophenol (PCP pesticide)
P 2604
Manufacturer
MSDS PDF LINK
87-86-5.pdf
CAS Number
87-86-5
Storage
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
R24 Toxic in contact with skin. R25 Toxic if swallowed.
R26 Very toxic by inhalation.
R36 Irritating to eyes. R37 Irritating to respiratory system.
R38 Irritating to skin. R40 Possible risk of irreversible effects.
R50 Very toxic to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
•
•
•
•
H319: Causes serious eye irritation
H315: Causes skin irritation
H330: Fatal if inhaled
H301: Toxic if swallowed
H311: Toxic in contact with skin
H335: May cause respiratory irritation
H351: Suspected of causing cancer
H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S22 Do not breathe dust. S36 Wear suitable protective clothing.
S37 Wear suitable gloves. S45 In case of accident or if you feel unwell,
seek medical advice immediately (show the label whenever possible.)
S52 Not recommended for interior use on large surface areas.
S60 This material and its container must be disposed of as hazardous
waste. S61 Avoid release to the environment. Refer to special instructions
/ safety data sheets.
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
P273: Avoid release to the environment. P280: Wear protective
gloves/protective clothing/eye protection/face protection. P284: Wear
respiratory protection. P301+310: IF SWALLOWED: Immediately call a
POISON CENTER or doctor/physician. P305+351+338: IF IN EYES: Rinse
continuously with water for several minutes. Remove contact lenses if
present and easy to do – continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
pregnancy alert
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Phenol
P 5566
BDH
Room Number
Risk Factors
MSDS PDF LINK
108-95-2.pdf
__ Date: 29-05-2013
CAS Number
108-95-2
Storage
Amount
100g
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation.
R24 Toxic in contact with skin. R25 Toxic if swallowed.
R34 Causes burns.
R48 Danger of serious damage to health by prolonged exposure.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed
•
H311: Toxic in contact with skin
•
H314: Causes severe skin burns and eye damage
•
H331: Toxic if inhaled
•
H341: Suspected of causing genetic defects
•
H373: May cause damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S24 Avoid contact with skin. S25 Avoid contact with eyes.
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S28 After contact with skin, wash immediately with plenty of soap-suds.
S36 Wear suitable protective clothing. S37 Wear suitable gloves.
S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36, 37, 38, 39, 41, 48
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional users only.PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Phenol SAT. Citrate Buffer
P 4682
Sigma
Room Number
Risk Factors
MSDS PDF LINK
p4682.pdf
__ Date: 29-05-2013
108-95-2 MIX
Storage
Fridge
CAS Number
Amount
R20 Harmful by inhalation. R21 Harmful in contact with skin.
R22 Harmful if swallowed. R23 Toxic by inhalation.
R24 Toxic in contact with skin. R25 Toxic if swallowed.
R34 Causes burns.
R48 Danger of serious damage to health by prolonged exposure.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed
•
H330: Fatal if inhaled
•
H314: Causes severe skin burns and eye damage
•
H331: Toxic if inhaled
•
H341: Suspected of causing genetic defects
•
H373: May cause damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing. S37 Wear suitable gloves.
S39 Wear eye / face protection.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection •
P284: Wear respiratory protection
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
•
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group C Toxic & Corrosive R23, 24, 25, 34, 35, 36, 37, 38, 39, 41, 48
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Restricted to trained professional usera only.PREGNANCY ALERT
Read Relevant Sections In Msds
Pipetting Heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
Phenol:Chlororform:Isoamylalcohol (25:24:1)
CAS Number
136112-00-0
P 3803
Storage
Sigma
Fridge sealed container
Amount
Risk Factors
R20 Harmful by inhalation. R21 Harmful in contact with skin. R22 Harmful
if swallowed.
R23 Toxic by inhalation. R24 Toxic in contact with skin R25 Toxic if
swallowed.
R34 Causes burns.
R40 Possible risk of irreversible effects.
136112-00-0.pdf
R48 Danger of serious damage to health by prolonged exposure.
R68 Possible risk of irreversible effects.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H301: Toxic if swallowed
•
H312: Harmful in contact with skin
•
H314: Causes severe skin burns and eye damage
•
H330: Fatal if inhaled
•
H341: Suspected of causing genetic defects
•
H351: Suspected of causing cancer
•
H373: May cause damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S39 Wear eye / face protection.
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection •
P284: Wear respiratory protection
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician • P305+351+338: IF IN EYES: Rinse continuously with
water for several minutes. Remove contact lenses if present and easy to
do – continue rinsing
•
P310: Immediately call a POISON CENTER or doctor/physician
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
2
1.5
1
4
12.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Two years of laboratory practicals. PREGNANCY ALERT
Read Relevant Sections in MSDS.
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Phenolphthalein
P 9750
BDH
CAS Number
77-09-8
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
100g
R45 May cause cancer.
R62 Risk of impaired fertility.
R68 Possible risk of irreversible effects.
77-09-8.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
H341: Suspected of causing genetic defects
H350: May cause cancer
H361: Suspected of damaging fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
•
P201: Obtain special instructions before use
P281: Use personal protective equipment as required
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Room Number
__ Date: 29-05-2013
Potassium dichromate
P2588
BDH
CAS Number
7778-50-9
Storage
Amount
500g
Risk Factors
R8 Contact with combustible material may cause fire. R21 Harmful in
contact with skin. R25 Toxic if swallowed. R26 Very toxic by inhalation.
R34 Causes burns. R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact. R45 May cause cancer.
R46 May cause heritable genetic damage R50 Very toxic to aquatic
organisms.
7778-50-9.pdf
R53 May cause long-term adverse effects in the aquatic environment.
R60 May impair fertility. R61 May cause harm to the unborn child.
LINK NOT ACTIVE HERE
MSDS PDF LINK
GHS HAZARD
STATEMENTS
•
H272: May intensify fire; oxidizer. H301: Toxic if swallowed
H312: Harmful in contact with skin •H314: Causes severe skin burns and
eye damage •H317: May cause an allergic skin reaction •H330: Fatal if
inhaled •H334: May cause allergy or asthma symptoms of breathing
difficulties if inhaled •H340: May cause genetic defects •H350: May cause
cancer •H360: May damage fertility or the unborn child •H372: Causes
damage to organs through prolonged or repeated exposure
•H410: Very toxic to aquatic life with long lasting effects
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
S60 This material and its container must be disposed of as hazardous
waste.
S61 Avoid release to the environment. Refer to special instructions /
safety data sheets.
•
P201: Obtain special instructions before use
•
P220: Keep/Store away from clothing/…/combustible materials
•
P260: Do not breathe dust/fume/gas/mist/vapours/spray
P273: Avoid release to the environment
•
P280: Wear protective gloves/protective clothing/eye
protection/face protection
•
P284: Wear respiratory protection
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS N Dangerous for the Environment
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Two years of laboratory practicals. PREGNANCY ALERT
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
__ Date: 29-05-2013
Rifampicin
R 3501
Sigma
CAS Number
13292-46-1
Storage
Room Number
Risk Factors
MSDS PDF LINK
Amount
R22 Harmful if swallowed.
R36 Irritating to eyes.
R37 Irritating to respiratory system.
R38 Irritating to skin.
13292-46-1.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
•
•
H302: Harmful if swallowed
H315: Causes skin irritation
H319: Causes serious eye irritation
H335: May cause respiratory irritation
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S26 In case of contact with eyes, rinse immediately with plenty of water
and seek medical advice.
S36 Wear suitable protective clothing.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P305+351+338: IF IN EYES: Rinse continuously with water for
several minutes. Remove contact lenses if present and easy to do –
continue rinsing
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
2
2.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Silane
333891
BDH
CAS Number
7803-62-5
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
25ml
R12 Extremely flammable.
R17 Spontaneously flammable in air.
R20 Harmful by inhalation.
7803-62-5.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H332: Harmful if inhaled
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S9 Keep container in a well-ventilated place.
S16 Keep away from sources of ignition.
S33 Take precautionary measures against static discharges.
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S39 Wear eye / face protection.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22
Flammable
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
2
4
8.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 5.1 -15 Suggests LOW RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
All users have undergone two years of laboratory practicals.
Read Relevant Sections in MSDS
Pipetting, heating
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Staurosporin
S 4400
Manufacturer
CAS Number
62996-74-1 + mixture
Storage
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
1mg
R45 May cause cancer.
R46 May cause heritable genetic damage.
62996-74-1.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
H340: May cause genetic defects
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S53 Avoid exposure - obtain special instructions before use.
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Streptomycin Sulphate
S 6501
Sigma
3810-74-0
Storage
Small Bottles
CAS Number
Room Number
Risk Factors
MSDS PDF LINK
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
R63 Possible risk of harm to the unborn child.
3810-74-0.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
•
H302: Harmful if swallowed
H361: Suspected of damaging fertility or the unborn child
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
P281: Use personal protective equipment as required
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
Group D Very Toxic or Mutagens R26, 27, 28 39, 40 48, 60, 61, 62. 63, 64
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
2
2.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Two years of laboratory practicals. PREGNANCY ALERT
Read Relevant Sections in MSDS
Weighing, pipetting
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Tetracycline
T 3258
Sigma
60-54-8
Storage
Freezer
CAS Number
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
R22 Harmful if swallowed.
MSDS PDF LINK
60-54-8.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H302: Harmful if swallowed
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S22 Do not breathe dust.
S36 Wear suitable protective clothing.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group B Harmful R 20, 21, 22, 33,40, 67
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
2
2.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read Relevant Sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Product Code
Manufacturer
Toluene
102846G 244511
BDH
Room Number
Risk Factors
MSDS PDF LINK
108-88-3.pdf
__ Date: 29-05-2013
108-88-3
Storage
Chemical cabinet
2500ml
Amount
CAS Number
R11 Highly flammable.
R38 Irritating to skin.
R20 Harmful by inhalation.
R48 Danger of serious damage to health by prolonged exposure.
R63 Possible risk of harm to the unborn child.
R65 Harmful: may cause lung damage if swallowed
R67 Vapours may cause drowsiness and dizziness
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H225: Highly flammable liquid and vapour
•
H304: May be fatal if swallowed and enters airways
•
H315: Causes skin irritation
•
H336: May cause drowsiness or dizziness
•
H361: Suspected of damaging fertility or the unborn child
•
H373: May cause damage to organs through prolonged or repeated
exposure
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S46 If swallowed, seek medical advice immediately and show this
container or label.
S62 If swallowed, do not induce vomitting; seek medical advice
immediately and show this container or label.
•
P210: Keep away from heat/sparks/open flames/hot surfaces – No
smoking
•
P261: Avoid breathing dust/fume/gas/mist/vapours/spray
•
P281: Use personal protective equipment as required
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or
doctor/physician
•
P331: Do NOT induce vomiting
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
HAZARD CLASS
__ Date: 29-05-2013
Group D Very Toxic or Mutagens R26, 27, 28, 48, 60, 61, 62. 63
Group S Skin/Eye Contact R21, 24, 27, 34,35,36, 38, 39, 40, 41, 43, 48, 66
OTHER HAZARD CLASS Flammable
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
2
1
1
2
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
PREGNANCY ALERT
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical name
Trypan blue
Product Code
T0776 T6146
Sigma
Manufacturer
72-57-1
Storage
Shelf
CAS Number
Room Number
Risk Factors
__ Date: 29-05-2013
Amount
25g
R45 May cause cancer.
MSDS PDF LINK
72-57-1.pdf
LINK NOT ACTIVE HERE
GHS HAZARD
STATEMENTS
•
H350: May cause cancer
Preventative Measures to be taken to reduce the level of risk:
Safety Factors
*****For Control
S45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label whenever possible.)
S53 Avoid exposure - obtain special instructions before use.
Arrangements read
relevant sections of
SOP # 3 and SOP
#8
GHS
PRECAUTIONARY
STATEMENTS
•
•
P201: Obtain special instructions before use
P308+313: IF exposed or concerned: Get medical advice/attention
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF CHEMICAL AGENTS
Safety, Health and Welfare at Work(Chemical Agents) Regulations 2001 - Regulation 4
Name and Status of Assessor : _____Prof. James O.Gara
Chemical Hazard Group
__ Date: 29-05-2013
Group E Sensitisers & Carcinogens R 40, 42, 45, 46, 49
HAZARD CLASS
OTHER HAZARD CLASS
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Hazard Rating
1
1
1
4
4.00
NEW HAZARD PICTOGRAMS
Grading Hazard
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating Numbers
Training Prerequisites
Emergency Action
Activites with Potential for Exposure
References if Any
Signature of Assessor
Read relevant sections in MSDS
DISCIPLINE OF MICROBIOLOGY
RISK ASSESMENT
OF
BIOLOGICAL HAZARDS
Please see Section 12 for the key to understanding how Risk Assessments were carried out in
the following section
Biological Agent Name
Biological
Agent Class
Location Room Number
Hazard
Rating
Number
Grading Hazard Rating Numbers
Aeromonas salmonicida
2
114
3
Rating 0 - 5 Suggests ACCEPTABLE RISK
Arcobacter spp.
2
212 215
0.6
Rating 0 - 5 Suggests ACCEPTABLE RISK
Bacillus subtilis
1
111 216 100P 225
0.5
Rating 0 - 5 Suggests ACCEPTABLE RISK
Bordetella bronchiseptica
2
214
6
Rating 5.1 - 15 Suggests LOW RISK
Bordetella pertussis
2
214
6
Rating 5.1 - 15 Suggests LOW RISK
Campylobacter coli
2
215
6
Rating 5.1 - 15 Suggests LOW RISK
Campylobacter jejuni
2
212 215
6
Rating 5.1 - 15 Suggests LOW RISK
Enterococcus faecalis
2
216 100P 225
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
Escherichia coli (non pathogenic
1
111 112 113 114 216 100P 215
225 212 213 214 ES104
0.75
Rating 0 - 5 Suggests ACCEPTABLE RISK
Helicobacter pylorii
2
6
Rating 5.1 - 15 Suggests LOW RISK
Listeria monocytogenes
2
113
6
Rating 5.1 - 15 Suggests LOW RISK
Pseudomonas aeruginosa
2
114 216 100P 225
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
Biological Agent Name
Biological
Agent Class
Location Room Number
Hazard
Rating
Number
Grading Hazard Rating Numbers
Salmonella typhimurium
2
111 114
6
Rating 5.1 - 15 Suggests LOW RISK
Serratia marcescens
1
216 100P 225
1
Rating 0 - 5 Suggests ACCEPTABLE RISK
Staphylococcus aureus
2
111 113 213 216 100P 225
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
Staphylococcus aureus MRSA
2
213
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
Streptococcus pyogenes
2
111 216 100P 225
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
Vibrio parahaemolyticus
2
214
2
Rating 0 - 5 Suggests ACCEPTABLE RISK
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Aeromonas salmonicida
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
114
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
1
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
3.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Arcobacter spp.
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
212 215
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers.
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
0.1
2
S
Hazard Rating Number
0.60
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Bacillus subtilis
Biological Agent Class
1
Location Room Number
Date: 24_May__2013
111 216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
1
P
0.1
1
S
Hazard Rating Number
0.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Bordetella bronchiseptica
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
214
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level 2 required when working with this microorganism:access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, a
class 2 lamina flow cabinet should be used when culturing this organism in conjunction with
gloves and masks,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Bordetella pertussis
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
214
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level 2 required when working with this microorganism:access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, a
class 2 lamina flow cabinet should be used when culturing this organism in conjunction with
gloves and masks,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Campylobacter coli
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
215
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Campylobacter jejuni
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
212 215
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Enterococcus faecalis
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Escherichia coli (non pathogenic strains)
Biological Agent Class
1
Location Room Number
Date: 24_May__2013
111 112 113 114 216 100P 215 225 212 213 214 ES104
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
1.5
P
0.1
1
S
Hazard Rating Number
0.75
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Helicobacter pylorii
Biological Agent Class
2
Date: 24_May__2013
Location Room Number
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level 2 required when working with this microorganism:access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, a
class 2 lamina flow cabinet should be used when culturing this organism in conjunction with
gloves and masks,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Listeria monocytogenes
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
113
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level 2 required when working with this microorganism:access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, a
class 2 lamina flow cabinet should be used when culturing this organism in conjunction with
gloves and masks,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
2
F NB Hazard Rating Number = N x F x P x S
1.5
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Pseudomonas aeruginosa
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
114 216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, This
organism is an opportunist pathogen which rarely if ever infects healthy tissue but once the
protection or barriers are broken down almost every tissue type is at risk of infection. avoid
contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Salmonella typhimurium
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
111 114
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level 2 required when working with this microorganism:access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers, a
class 2 lamina flow cabinet should be used when culturing this organism in conjunction with
gloves and masks,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
3
F NB Hazard Rating Number = N x F x P x S
1
P
1
2
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Serratia marcescens
Biological Agent Class
1
Location Room Number
Date: 24_May__2013
216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
1
S
Hazard Rating Number
1.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Staphylococcus aureus
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
111 113 213 216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Staphylococcus aureus MRSA
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
213
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Streptococcus pyogenes
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
111 216 100P 225
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
5
F NB Hazard Rating Number = N x F x P x S
0.2
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Undergraduate Use In Supervised Practical Class Environment.
Postgraduate Use In Supervised Microbiology Research
Laboratories.
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Biological Agent Name
Vibrio parahaemolyticus
Biological Agent Class
2
Location Room Number
Date: 24_May__2013
214
Biological Risk Groups
Group 1 : Unlikely to cause disease
Group 2: May cause human disease, may be hazardous,unlikely to spread, but is treatable,
Group 3: May cause severe human disease, presents serious hazard, may spread, but is treatable
Group 4: Causes severe human disease, is a serious hazard, presents high risk of spreading and
4 contd.: has no effective treatment
InfectionOutcome
Unlikely to cause a Hazard
Possible gastritis
Lung or airway infection
Skin, wound or ulcer infection
Infection of blood possibly leading to septicemia
A: Possible Allergic Effects
D: List of workers exposed to this agent to be kept for 10 years-after last known exposure
T: Toxin Exposure
V: Effective vaccine available
It may induce cancer
It may endanger the foetus in pregnant women
Preventative Measures to be taken to reduce the level of risk:
Safety
Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Containment level required :- Basic Microbiological Aseptic Handling Techniques.
access restricted to authorised personell, doors should contain a small glass observation panel,
specified disinfection procedures must be in place, recommended to have effective vector
control (rodents, insects), benches must be impervious to water and easy to clean, work
surfaces should resist chemicals, all unused cultures to be autoclaved and sent for ecosafe
disposal, cultures should be stored in cold rooms or freezers in clearly labeled containers,
avoid contact by skin or eyes and do not create or breathe aerosols.
N
1
F NB Hazard Rating Number = N x F x P x S
1
P
1
2
S
Hazard Rating Number
2.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF BIOLOGICAL AGENTS
Safety, Health and Welfare at Work Biological Agents Regulations 1994/1998
Name and Status of Assessor : Prof. JamesO’Gara
Grading
Hazard
Rating
Numbers
Date: 24_May__2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Only qualified workers with experience of Microbiological Aseptic
Handling Techniques should work with these organisms
Resources
Activites with
Potential for
Exposure
Working with cuts or open wounds without using sealed dressing
and rubber gloves.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP #1, SOP # 2, SOP # 3, SOP # 4,SOP # 5,SOP # 6, SOP
# 7, SOP #13
DISCIPLINE OF MICROBIOLOGY
RISK ASSESMENT
OF
EQUIPMENT AND PROCEDURES
Please see Section 12 for the key to understanding how Risk Assessments were carried out in
the following section
Hazard
Rating
Number
Equipment or Procedure
Location Room Number
Grading Hazard Rating Numbers
Autoclaves (including bench type)
107 111 113 114 116 216 217 225
ES103 ES104
Balances
112 113 114 213 214 216 217 ES102
ES103 ES104)
Broken Glass
All Laboratories
4.50
Bunsen Burner or Naked Flames
All Laboratories
10.00
Rating 5.1 - 15 Suggests
LOW RISK
Centrifuges (including bench type)
111 112 113 114 116 211 213 214 215
216 ES103 ES104 ES109
7.50
Rating 5.1 - 15 Suggests
LOW RISK
Chemical storage cabinets or shelves
108(ducted) 220 (ducted) 110 111 112
113 114 126 212 213 214 215 216 217
ES103 ES104
15.00
Rating 5.1 - 15 Suggests
LOW RISK
Class 2 Biological Safety Cabinets
108 111 112 114 116b 213 215
4.50
Rating 0 - 5 Suggests
ACCEPTABLE RISK
Computer Terminals or VDU
All Rooms
5.00
Rating 0 - 5 Suggests
ACCEPTABLE RISK
Electrophoresis Equipment
111 112 113 114 204 212 213 214
ES103
7.50
Rating 5.1 - 15 Suggests
LOW RISK
15.00
Rating 5.1 - 15 Suggests
LOW RISK
15.00
Rating 0 - 5 Suggests
ACCEPTABLE RISK
Rating 5.1 - 15 Suggests
LOW RISK
Hazard
Rating
Number
Equipment or Procedure
Location Room Number
Grading Hazard Rating Numbers
Freezers Cold Rooms Fridges
111 112 113 114 120 212 213 214 215
217221 224 ES (101 103 104)
15.00
Rating 5.1 - 15 Suggests
LOW RISK
Fume Extractor Hoods
113 116 204 217 ES102 ES103 ES104
ES204
6.00
Rating 5.1 - 15 Suggests
LOW RISK
Gas or Liquid Chromotography
ES109 118
10.00
Rating 5.1 - 15 Suggests
LOW RISK
General Electrical Equipment
All Rooms
15.00
Rating 5.1 - 15 Suggests
LOW RISK
Handling Pathological Materials
All laboratories
7.50
Rating 5.1 - 15 Suggests
LOW RISK
Hot plates Hot ovens Thermal Cyclers
111 112 113 114 116 204 213 214 215
216 217 ES102 ES103 ES104
15.00
Rating 5.1 - 15 Suggests
LOW RISK
Hot Rooms Controlled Temperature Rooms
121 222 223
7.50
Rating 5.1 - 15 Suggests
LOW RISK
Manual Handling
All Rooms
10.00
Rating 5.1 - 15 Suggests
LOW RISK
Microscopes
All Laboratories
0.75
Rating 0 - 5 Suggests
ACCEPTABLE RISK
Hazard
Rating
Number
Equipment or Procedure
Location Room Number
Sharps
All Laboratories
Storage Cabinets and Shelving
All Rooms
Grading Hazard Rating Numbers
7.50
Rating 5.1 - 15 Suggests
LOW RISK
15.00
Rating 5.1 - 15 Suggests
LOW RISK
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Autoclaves (including bench type)
107 111 113 114 116 216 217 225 ES103 ES104
Hot surfaces and Steam pressurised
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Use gloves for hot items
Observe safe handling AND lifting technique when placing materials into autoclave.
The H&S office organise courses for all staff and researchers in safe handling techniques.
Ensure caps are loose on all bottles to allow pressure to equalise.
Any malfunction or leakage is reported immediatly to technical staff for correction.
1
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
7.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
All new users must be instructed by senior lab scientist
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
Do not autoclave volatile liquids or sealed containers
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Regular servicing by professional engineers of safety valves and
controls are carried out and regular inspection of autoclaves by the
universities insurance company is also carried out. Autoclaves are
replaced by new models when the begin to show signs of wear or
fatigue.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Balances
112 113 114 213 214 216 217 ES102 ES103 ES104)
Fine powder forming aerosols
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Use mask and gloves when weighing out materials.
Read Chemical Risk Assesment and Material Safety Data Sheets on Chemical being weighed
before you proceed to weigh out any unfamiliar chemical.
Clean balance after every use.
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Before using any Chemicals and Biological Reagents users must
read the relevant material safety data sheets supplied by Retailer
and available in the laboratory .
Check Departmental Chemical Rik Assessment File which is
available on computer file in research laboratories.
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Avoid creating aerosols
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read all relevant SOP’s # 1 to #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Broken Glass
All Laboratories
Broken or cracked glassware and pipettes
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All broken glass must be disposed of directly into designated broken glass container. Ensure
contaminated glassware is autoclaved before disposal.
Currently all broken glass along with other non-biohazardous waste is collected by outside
contractor ( Namely CITY BIN WASTE DISPOSAL) for approved land fill disposal. Update as
frequently as necessary on anti-tetanus injections if injury was sustained.
1
1.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
4.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
No specific training required, just an awareness of being careful
when handling broken glass is required
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
handling hot glassware giving rise to thermal shock which can lead
to glass breaking
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Use gloves and container to remove broken pieces of glass
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Bunsen Burner or Naked Flames
All Laboratories
Leaky or worn flexible gas tubing
Fire
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Avoid naked flames near flammable substances, explosives and volatile liquids
Use oven gloves when handling hot items
Locate bunsens in a safe area on work bench
Check that valves are turned off correctly
1
2.5
1
4
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
10.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
General instruction in the safe use of naked flames.
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Heating liquids in glass containers. Sterilizing spreaders and loops
used for culturing microorganisms.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Centrifuges (including bench type)
111 112 113 114 116 211 213 214 215 216 ES103 ES104 ES109
Rotor imbalance
Spillage
aerosol
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Always balance items to be centrifuged
Ensure any caps are secured properly
Ensure tubes are correct fit for rotor
Always use correct speed for type of rotor in use
Report any fault or unusual occurence
All centrifuges have safety locks to prevent lids from opening before rotor stops
Read SOP #1
1
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
7.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Instruction in the use of equipment by senior Lab personell
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
Poorly fitting centrifuge tubes or caps. Tubes which are not balanced
accurately.
Health Surveillance
Conclusions
Recommended
Controls if Any
Ensure instruction is given to all new students before they use a
centrifuge.
Read SOP #7
Signature of Assessor
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Prof. JamesO’Gara
__ Date: 24-May-2013
Chemical storage cabinets or shelves
108(ducted) 220 (ducted) 110 111 112 113 114 126 212 213 214 215 216 217
ES103 ES104
Hazard Risk Factors
Hazard Outcome
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Chemicals must be stored centrally in approved containers and in ventilated chemical cabinets
where possible. Read and keep the safety data sheets for chemicals in stock and particularly
read MSDS before using any chemical. Use gloves and masks where required. Use in well
ventilated areas or fume hoods. Dispose of correctly, according to manufacturers instructions.
Separate stored chemicals such as Solvents, Oxidisers, Acids, Combustibles and Flammables.
Do not over load shelving. Keep heavier items on lower shelves.
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Three years experience in laboratory environment leading to a third
level qualification in science should be a minimum requirement for
access to chemical storage areas.
Safety , Health and Welfare at Work(Chemical Agents) Regulations
2001, Dangerous Substances Regulations 1990, Senior Post
Graduate Research Students, Technical Staff and Research
Supervisors
Overloading of shelving and storing incompatible chemicals near
each other.
Health Surveillance
Conclusions
Recommended
Controls if Any
Risk assessment of chemicals being stored together should be
carried out by senior scientist or supervisor
Read relevant MSDS information on each Chemical before storing
next to other chemicals.
Signature of Assessor
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Class 2 Biological Safety Cabinets
108 111 112 114 116b 213 215
Possible Exposure to Pathogenic Material
Cross Contamination
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All filters and exhaust sysyems are maintained professionally on a regular basis.
Work surfaces must be sterilised between users using available disinfectants.
Masks glasses and gloves must be worn at all times when working at the cabinet. Spills must
be mopped up using absorbent material and the material must be placed in autoclave bag and
autoclaved, the area cleaned thoroughly by readily available sterilising agents.
1
1.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
4.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
All new users must be instructed by senior lab scientist
Manufacturers Manuals. Safety , Health and Welfare at Work
(Biological Agents) Regulations 1994 and 1998. Senior Post
Graduate Research Students, Technical Staff and Research
Supervisors
Culturing, and extracting DNA /RNA / protein / lipid etc. from
cultures.
Health Surveillance
Conclusions
Recommended
Controls if Any
Class 2 Pathogenic Organisms must be handled in this facility
Read SOP #4 and # 14
Signature of Assessor
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Computer Terminals or VDU
All Rooms
Trailing cables
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Approved seating and posture for long term users
Use cable tidies where required
1
2.5
1
2
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
5.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Assessment of Computer Terminal position is provided by NUI,
Galway VDU Assessors
Local Assessor Dr. Ger Fleming.
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
Improper seating and location of VDU Screen and Keyboard leading
to RSI.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Screens can be provided to avoid glare where required. Convert to
using TFT type flat screen (liquid crystal rather than a monitor).
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Electrophoresis Equipment
111 112 113 114 204 212 213 214 ES103
High Voltage and Direct Current
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Always ensure power supply is switched off before handling.
All equipment must be connected to ELCB / RCD switched Elevtrical Breakers.
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
All new users must be instructed by senior lab scientist
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Freezers Cold Rooms Fridges
111 112 113 114 120 212 213 214 215 217221 224 ES (101 103 104)
Breakages Spillages
Freezer burns
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Ensure all containers are clearly labelled as to contents and ownership
Use gloves for frozen materials
Use containers suitable for long term freezing
All equipment must be connected to ELCB / RCD switched Elevtrical Breakers.
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
No specific training required
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Fume Extractor Hoods
113 116 204 217 ES102 ES103 ES104 ES204
Fume hoods are used when handling particularly volatile or toxic chemicals for
example during distillation and separation techniques. Exposure to toxic fumes
and potential explosions are possible.
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Gloves and masks must be worn when required.
Risk assessment must be carried out on all experiments and procedures using chemicals in an
extractor hood before they are carried out.
1
1.5
1
4
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
6.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
All new users must be instructed by senior lab scientist
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Any chemical reaction which can liberate toxic fumes.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Risk assessment of procedure requiring use of a fume hood should
be carried out by senior scientist or supervisor
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Gas or Liquid Chromotography
ES109 118
Gas leakage
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All gas cylinders are located outside and are piped into Room ES109 under low pressure
1
2.5
1
4
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
10.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
only graduates or fourth year students under supervision use these
GC's
manufacturers manuals
Senior post graduate researchers
technical staff
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
General Electrical Equipment
All Rooms
Handling or relocating equipment
Trailing Cables
Old or worn out equipment
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All Electrical pieces of quipment in use are connected to ELCB / RCD Residual Current
Detectors
If using any power tools be sure to use all safety Guards supplied by manufacturer.
Ensure all equipment is serviced on a regular basis.
Report any faulty equipment to a supervisor.
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Specific training may be required for some electrical equipment.
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Check with supervisor before using new equipment, read any
relevant SOP or Instruction Manuals provided.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Handling Pathological Materials
All laboratories
Pathological Materials Bacteria Viruses
Blood and other potentially infectious material
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Use of good aseptic handling techniques as demonstrated in undergraduate practical classes.
Use gloves and masks when required. Use laminar flow filtered cabinets for class 2 pathogens
when required. An EPA license to handle Class 2 pathogens has to be purchased by
laboratories using Class 2 pathogens. Autoclave all pathogenic culture media and contaminated
materials before approved disposal methods for biological waste are employed (namely ECO
SAFE) . Personnel using pathological material should if required and available ensure they have
all the vaccinations deemed necessary to work with such material.
1
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
7.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Only trained personnel should handle pathological material
Environmental Protection Agency. Manufacturers Manuals. Safety ,
Health and Welfare at Work(Biological Agents) Regulations 1994
and 1998. Senior Post Graduate Research Students, Technical Staff
and Research Supervisors
Sub-culturing, centrifuging and extracting DNA /RNA / protein / lipid
etc. from cultures.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read all SOP relating to handling Biological Materials
SOP’s #1,#2, #3, #4, #5, #6, #7, #13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Hot plates Hot ovens Thermal Cyclers
111 112 113 114 116 204 213 214 215 216 217 ES102 ES103 ES104
Glass Bottles and Containers when hot may break allowing contents to leak
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Use Gloves when handling Hot Items
Avoid sudden temperature changes or moving from hot to cold surfaces
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
All new users must be instructed by senior lab scientist
Manufacturers Manuals
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Hot surfaces should be clearly labeled
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Hot Rooms Controlled Temperature Rooms
121 222 223
Aerosols from platform Shakers
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Ensure all containers are tightly capped
If containers are agitated on shakers ensure they are secured to the platform
Spillages if they occur should be contained using the spill kits available in each laboratory and
the area cleaned with fresh disinfectant such as 70% Industrial Methylated Spirit or Virkon
which is also available in all research labs.
Switch off shaking platforms before removing or adding containers
1
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
7.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
No specific training required
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Aerosol creation from aeration. Using rotating or shaking platforms
in hotrooms
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Manual Handling
All Rooms
Muscle or other injury through bad lifting skills
Moving or lifting laboratory equipment, compressed gas bottles etc.
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All personell who are involved in manual handling should enroll in a suitable course.
1
2
1
5
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
10.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
All staff and student researchers involved in manual handling must
attend a course in Manual Handling to be repeated every three
years.
Health and Safety course are organised by the Health and Safety
office for information follow this link
http://www.nuigalway.ie/healthsaf/index.php?menu=4&page=66
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
An active register of all Health and Safety Courses attended by staff
and researchers must be maintained and repeat attendances for
individuals should be notified.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Microscopes
All Laboratories
Poor posture for prolonged periods
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Ensure comfortable seating and posture
Focus each lens for each eye separately to avoid eye strain
5
1.5
0.1
1
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
0.75
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
Activites with
Potential for
Exposure
Training in the use of a Microscope is carried out at undergraduate
level.
Manufacturers Manuals, Senior Post Graduate Research Students,
Technical Staff and Research Supervisors
Carrying a Microscope from one location to another. Improper
seating and posture while using a microscope.
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Annual maintenance of microscopes and checking of electric cables
and plugs for wear.
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Sharps
All Laboratories
Needles scalpels and other sharp implements can cause puncture wounds and
infection
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
All sharps must be disposed of directly into designated sharps container. Do not attempt to resheath sharps when they are biohazardous. Dispose of sharps in yellow sharps disposal bins.
Currently all bins with sharps material are collected by outside contractor (namely ECO SAFE)
for approved disposal.
Update as frequently as necessary on Anti-Tetanus injections and Anti-Hepatitis if applicable
1
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
7.50
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
Resources
No specific training required, just an awareness of being careful
when handling sharps such as needles blades and pointed
instruments is required so as not to stab or puncture skin of any
person.
Senior Post Graduate Research Students, Technical Staff and
Research Supervisors
Activites with
Potential for
Exposure
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Read SOP # 2, # 13
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Equipment or Procedure
Location Room Number
Hazard Risk Factors
Hazard Outcome
Prof. JamesO’Gara
__ Date: 24-May-2013
Storage Cabinets and Shelving
All Rooms
Collapsing shelves
Unsecured Cabinets topplig over
Chemical spills from items falling
No Apparent Hazard
Muscle Strain (Rep. Strain Injury)
Burns or Scalds
Physical Injury
Electric Shock
Exposure to Chemical or Biological Hazard
Involving a Flammable or Explosive Procedure
Preventative Measures to be taken to reduce the level of risk:
Safety Control
Measures
Number of Users at Risk N
Frequency of use F
Probability of Mishap P
Severity of Mishap S
Secure any cabinet which could topple over if top heavy.
Ensure shelving is suitable for loads being stored.
Store heavy items at low levels.
Use appropriate lifting equipment when required
Do not store incompatable chemicals together Eg. Oxidisers with Flammables
2
2.5
1
3
N
F
NB Hazard Rating Number = N x F x P x S
P
S
Hazard Rating Number
15.00
DISCIPLINE OF MICROBIOLOGY, National University of Ireland, Galway
RISK ASSESSMENT OF EQUIPMENT AND PROCEDURES
Safety, Health and Welfare at Work Regulations 1993/2005
Name and Status of Assessor :
Grading
Hazard
Rating
Numbers
Prof. JamesO’Gara
__ Date: 24-May-2013
Rating 0 - 5 Suggests ACCEPTABLE RISK
Rating 5.1 - 15 Suggests LOW RISK
Rating 15.1 - 40 Suggests SIGNIFICANT RISK
Rating 40.1 - 100 Suggests HIGH RISK
Rating > 100 Suggests EXTREME RISK
Training Prerequisites
University provides instruction in safe handling, lifting and carrying
techniques.
Resources
Activites with
Potential for
Exposure
Attempting to lift heavy objects without sufficient help or necessary
lifting equipment
Health Surveillance
Conclusions
Recommended
Controls if Any
Signature of Assessor
Check with supervisor before using new equipment, read any
relevant SOP or Instruction Manuals provided.
Over 1500 chemicals in common use in Microbiology have been Risk Assessed.
The full risk assesment of all our chemicals printed out would require >6000 pages of print to
cover all information collated. This is available in PDF format on several PC platforms in the
laboratories.
We have included the full list of chemicals in common use in Microbiology and the Full Risk
Assessments on a sample of chemicals and all of the chemicals which pose a risk to Pregnancy.
The active DATA BASE TEMPLATE used to determine the Risk Assessments was designed
using FileMaker Pro by Claris.
This data base can be used easily on both PC and Mac platforms and can be easily updated on a
regular basis and can be used to do an Instant Risk Assessment on any procedure or experiment.
HAZARD RATING NUMBER
THIS IS THE FORMULA TO SHOW HOW THE HAZARD RATING NUMBER VALUES WERE DERIVED
USING THE FileMakerPro DATA BASE TEMPLATE
N x F x P x S = HAZARD RATING NUMBER
WHERE
NUMBER OF USERS AT RISK
= N
Where N is aVALUE from 1 to 5 decided on by the following scale;
1 to 2 Persons Enter 1
3 to 7 Persons Enter 2
8 to 15 Persons Enter 3
16 to 50 Persons Enter 4
More than 50 Enter 5
FREQUENCY OF USE
= F
Where F is a value from 0.1 to 5 decided on by the following scale
< Annually Enter 0.1
Annually Enter 0.2
Monthly Enter 1
Weekly Enter 1.5
Daily Enter 2.5
Hourly Enter 4
Continuously Enter 5
PROBABILITY OF MISHAP
= P
Where P is a value from 0.1 to 6 decided on by the following scale
Impossible or Inconceivable Enter 0.1
Unlikely Enter 1
Possible Enter 2
Even chance Enter 3
Probable Enter 4
Likely Enter 5
Certain Enter 6
SEVERITY OF MISHAP
= S For Equipment and Procedures
Where S is a value from 0.1 to 5 decided on by the following scale
No Apparent Hazard > Enter value 0.1
Muscle Strain RSI > Enter value 0.5 to 1
Burns or Scalds > Enter value 1 to 2
Physical Injury (Power tools, Sharps etc.) > Enter value 2 to 3
Electric Shock > Enter value 2 to 5
Exposure to Chemical or Biological Hazard > Enter value 3 to 5
Involving a Flammable or Explosive Procedure > Enter value 4 to 5
Please Gauge The Value to be assigned by the level of Risk and Exposure involved
Double click the box and type the value to be assigned
SEVERITY OF MISHAP
=S
For Chemical Hazards
No Apparent Hazard > Enter value 0.5
Flammable or Explosive see R 1 to 19 > Enter value 1 to 5
Hazard Group A Irritant > Enter value1 to 2
Hazard Group B Harmful > Enter value1 to 3
Hazard Group C Toxic & Corrosive > Enter value1 to 4
Hazard Group D Very Toxic & Mutagens > Enter value 2 to 4
Hazard Group E Sensitisers & Carcinogens > Enter value 4
Please Gauge The Value to be assigned by Amount used, Volatility, Dustiness
and the level of Safety Control Approach Required
SEVERITY OF MISHAP
=S
For Biological Hazard
Please Assign the Value the same as the Biological Risk Group Number 1-4
Double click the box and type the value to be assigned
RATING 0-5 SUGGESTS ACCEPTABLE RISK
RATING 5.1-15 SUGGESTS LOW RISK
RATING 15.1- 40 SUGGESTS SIGNIFICANT RISK
RATING 40.1- 100 SUGGESTS HIGH RISK
RATING > 100 SUGGESTS EXTREME RISK
RISK AND SAFETY FACTORS
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R1 Explosive when dry.
R2 Risk of explosion by shock, friction, fire or other source of ignition.
R3 Extreme risk of explosion by shock, friction, fire or other sources of ignition.
R4 Forms very sensitive explosive metallic compounds.
R5 Heating may cause an explosion.
R6 Explosive with or without contact with air.
R7 May cause fire.
R8 Contact with combustible material may cause fire.
R9 Explosive when mixed with combustible material.
R10 Flammable.
R11 Highly flammable.
R12 Extremely flammable.
R13 Extremely flammable liquefied gas
R14 Reacts violently with water.
R15 Contact with water liberates extremely flammable gases.
R16 Explosive when mixed with oxidizing substances.
R17 Spontaneously flammable in air.
R18 In use, may form inflammable/explosive vapour-air mixture.
R19 May form explosive peroxides.
R20 Harmful by inhalation.
R21 Harmful in contact with skin.
R22 Harmful if swallowed.
R23 Toxic by inhalation.
R24 Toxic in contact with skin.
R25 Toxic if swallowed.
R26 Very toxic by inhalation.
R27 Very toxic in contact with skin.
R28 Very toxic if swallowed.
R29 Contact with water liberates toxic gas.
R30 Can become highly flammable in use.
R31 Contact with acids liberates toxic gas.
R32 Contact with acid liberates very toxic gas.
R33 Danger of cumulative effects.
R34 Causes burns.
R35 Causes severe burns.
R36 Irritating to eyes.
R37 Irritating to respiratory system.
R38 Irritating to skin.
R39 Danger of very serious irreversible effects.
R40 Possible risk of irreversible effects.
R41 Risk of serious damage to the eyes.
R42 May cause sensitization by inhalation.
R43 May cause sensitization by skin contact.
R44 Risk of explosion if heated under confinement.
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R45 May cause cancer.
R46 May cause heritable genetic damage.
R47 May cause birth defects.
R48 Danger of serious damage to health by prolonged exposure.
R49 May cause cancer by inhalation.
R50 Very toxic to aquatic organisms.
R51 Toxic to aquatic organisms.
R52 Harmful to aquatic organisms.
R53 May cause long-term adverse effects in the aquatic environment.
R54 Toxic to flora.
R55 Toxic to fauna.
R56 Toxic to soil organisms.
R57 Toxic to bees.
R58 May cause long-term adverse effects in the environment.
R59 Dangerous to the ozone layer.
R60 May impair fertility.
R61 May cause harm to the unborn child.
R62 Risk of impaired fertility.
R63 Possible risk of harm to the unborn child.
R64 May cause harm to breastfed babies.
R65 Harmful: may cause lung damage if swallowed
R66 Repeated exposure may cause skin dryness or cracking
R67 Vapours may cause drowsiness and dizziness
R68 Possible risk of irreversible effects.
Potential Health Effects
Inhalation
May be harmful if inhaled. May cause respiratory tract irritation.
Skin
May be harmful if absorbed through skin. May cause skin irritation.
Eyes
May cause eye irritation.
Ingestion
May be harmful if swallowed.
Exercise appropriate precautions to minimize direct contact with skin or eyes and prevent inhalation.
Avoid inhalation.
Avoid contact with eyes, skin, and clothing.
Avoid prolonged or repeated exposure.
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S1 Keep locked up.
S2 Keep out of the reach of children.
S3 Keep in a cool place.
S4 Keep away from living quarters.
S5 Keep contents under ... (there follows the name of a liquid).
S6 Keep under ... (there follows the name of an inert gas).
S7 Keep container tightly closed.
S8 Keep container dry.
S9 Keep container in a well-ventilated place.
S12 Do not keep the container sealed.
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S13 Keep away from food, drink and animal foodstuffs.
S14 Keep away from ... (a list of incompatible materials will follow).
S15 Keep away from heat.
S16 Keep away from sources of ignition.
S17 Keep away from combustible material.
S18 Handle and open container with care.
S20 When using, do not eat or drink.
S21 When using do not smoke.
S22 Do not breathe dust.
S23 Do not breathe vapour.
S24 Avoid contact with skin.
S25 Avoid contact with eyes.
S26 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S27 Take off immediately all contaminated clothing.
S28 After contact with skin, wash immediately with plenty of soap-suds.
S29 Do not empty into drains.
S30 Never add water to this product.
S33 Take precautionary measures against static discharges.
S35 This material and its container must be disposed of in a safe way.
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S36 Wear suitable protective clothing.
S37 Wear suitable gloves.
S38 In case of insufficient ventilation, wear suitable respiratory equipment.
S39 Wear eye / face protection.
S40 To clean the floor and all objects contaminated by this material, use .... (there follows suitable cleaning material).
S41 In case of fire and / or explosion do not breathe fumes.
S42 During fumigation / spraying wear suitable respiratory equipment.
S43 In case of fire use ... (there follows the type of fire-fighting equipment to be used.)
S45 In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S46 If swallowed, seek medical advice immediately and show this container or label.
S47 Keep at temperature not exceeding...
S48 To be kept wet with (there follows a material name).
S49 Keep only in the original container.
S50 Do not mix with ...
S51 Use only in well ventilated areas.
S52 Not recommended for interior use on large surface areas.
S53 Avoid exposure - obtain special instructions before use.
S56 Dispose of this material and its container at hazardous or special waste collection point.
S57 Use appropriate container to avoid environmental contamination.
S59 Refer to manufacturer / supplier for information on recovery / recycling.
S60 This material and its container must be disposed of as hazardous waste.
S61 Avoid release to the environment. Refer to special instructions / safety data sheets.
S62 If swallowed, do not induce vomitting; seek medical advice immediately and show this container or label.
S63 In case of accident by inhalation, remove casualty to fresh air and keep at rest
S64 If swallowed, rinse mouth with water (only if the person is conscious)
GLOBALLY HARMONISED SYSTEM OF CLASSIFICATION
AND LABELLING OF CHEMICALS
Physical hazards
•
H200: Unstable explosive
•
H201: Explosive; mass explosion hazard
•
H202: Explosive; severe projection hazard
•
H203: Explosive; fire, blast or projection hazard
•
H204: Fire or projection hazard
•
H205: May mass explode in fire
•
H220: Extremely flammable gas
•
H221: Flammable gas
•
H222: Extremely flammable material
•
H223: Flammable material
•
H224: Extremely flammable liquid and vapour
•
H225: Highly flammable liquid and vapour
•
H226: Flammable liquid and vapour
•
H227: Combustible liquid
•
H228: Flammable solid
•
H240: Heating may cause an explosion
•
H241: Heating may cause a fire or explosion
•
H242: Heating may cause a fire
•
H250: Catches fire spontaneously if exposed to air
•
H251: Self-heating; may catch fire
•
H252: Self-heating in large quantities; may catch fire
•
H260: In contact with water releases flammable gases which may ignite spontaneously
•
H261: In contact with water releases flammable gas
•
H270: May cause or intensify fire; oxidizer
•
H271: May cause fire or explosion; strong oxidizer
•
H272: May intensify fire; oxidizer
•
H280: Contains gas under pressure; may explode if heated
•
H281: Contains refrigerated gas; may cause cryogenic burns or injury
•
H290: May be corrosive to metals
[edit] Health hazards
•
H300: Fatal if swallowed
•
H301: Toxic if swallowed
•
H302: Harmful if swallowed
•
H303: May be harmful if swallowed
•
H304: May be fatal if swallowed and enters airways
•
H305: May be harmful if swallowed and enters airways
•
H310: Fatal in contact with skin
•
H311: Toxic in contact with skin
•
H312: Harmful in contact with skin
•
H313: May be harmful in contact with skin
•
H314: Causes severe skin burns and eye damage
•
H315: Causes skin irritation
•
H316: Causes mild skin irritation
•
H317: May cause an allergic skin reaction
•
H318: Causes serious eye damage
•
H319: Causes serious eye irritation
•
H320: Causes eye irritation
•
H330: Fatal if inhaled
•
H331: Toxic if inhaled
•
H332: Harmful if inhaled
•
H333: May be harmful if inhaled
•
H334: May cause allergy or asthma symptoms of breathing difficulties if inhaled
•
H335: May cause respiratory irritation
•
H336: May cause drowsiness or dizziness
•
H340: May cause genetic defects
•
H341: Suspected of causing genetic defects
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H350: May cause cancer
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H351: Suspected of causing cancer
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H360: May damage fertility or the unborn child
•
H361: Suspected of damaging fertility or the unborn child
•
H362: May cause harm to breast-fed children
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H370: Causes damage to organs
•
H371: May cause damage to organs
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H372: Causes damage to organs through prolonged or repeated exposure
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H373: May cause damage to organs through prolonged or repeated exposure
[edit] Environmental hazards
•
H400: Very toxic to aquatic life
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H401: Toxic to aquatic life
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H402: Harmful to aquatic life
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H410: Very toxic to aquatic life with long lasting effects
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H411: Toxic to aquatic life with long lasting effects
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H412: Harmful to aquatic life with long lasting effects
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H413: May cause long lasting harmful effects to aquatic life
[edit] Country-specific hazard statements
[edit] European Union
The European Union has implemented the GHS through the CLP Regulation. Nevertheless, the older system based on the Dangerous Substances Directive will continue to be used in
parallel until 2016. Some R-phrases which do not have simple equivalents under the GHS have been retained under the CLP Regulation:[5] the numbering mirrors the number of the
previous R-phrase.
[edit] Physical properties
•
EUH001: Explosive when dry
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EUH006: Explosive with or without contact with air
•
EUH014: Reacts violently with water
•
EUH018: In use may form flammable/explosive vapour-air mixture
•
EUH019: May form explosive peroxides
•
EUH044: Risk of explosion if heated under confinement
[edit] Health properties
•
EUH029: Contact with water liberates toxic gas
•
EUH031: Contact with acids liberates toxic gas
•
EUH032: Contact with acids liberates very toxic gas
•
EUH066: Repeated exposure may cause skin dryness or cracking
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EUH070: Toxic by eye contact
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EUH071: Corrosive to the respiratory tract
[edit] Environmental properties
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EUH059: Hazardous to the ozone layer
General precautionary statements
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P101: If medical advice is needed, have product container or label at hand
•
P102: Keep out of reach of children
•
P103: Read label before use
[edit] Prevention precautionary statements
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P201: Obtain special instructions before use
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P202: Do not handle until all safety precautions have been read and understood
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P210: Keep away from heat/sparks/open flames/hot surfaces – No smoking
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P211: Do not spray on an open flame or other igntion source
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P220: Keep/Store away from clothing/…/combustible materials
•
P221: Take any precaustion to avoid mixinn with combustibles
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P222: Do not allow contact with air
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P223: Keep away from any possible contact with water, because of violent reaction and possible flash fire
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P230: Keep wetted with …
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P231: Handle under inert gas
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P232: Protect from moisture
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P233: Keep container tightly closed
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P234: Keep only in original container
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P235: Keep cool
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P240: Ground/bond container and receiving equipment
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P241: Use explosion-proof electrical/ventilating/light/…/equipment
•
P242: Use only non-sparking tools
•
P243: Take precautionary measures against static discharge
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P244: Keep reduction valves free from grease and oil
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P250: Do not subject to grinding/shock/…/friction
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P251: Pressurized container – Do not pierce or burn, even after use
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P260: Do not breathe dust/fume/gas/mist/vapours/spray
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P261: Avoid breathing dust/fume/gas/mist/vapours/spray
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P262: Do not get in eyes, on skin, or on clothing
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P263: Avoid contact during pregnancy/while nursing
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P264: Wash … thoroughly after handling
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P270: Do not eat, drink or smoke when using this product
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P271: Use only outdoors or in a well-ventilated area
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P272: Contaminated work clothing should not be allowed out of the workplace
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P273: Avoid release to the environment
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P280: Wear protective gloves/protective clothing/eye protection/face protection
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P281: Use personal protective equipment as required
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P282: Wear cold insulating gloves/face shield/eye protection
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P283: Wear fire/flame resistant/retardant clothing
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P284: Wear respiratory protection
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P285: In case of inadequate ventilation wear respiratory protection
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P231+232: Handle under inert gas. Protect from moisture
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P235+410: Keep cool. Protect from sunlight
[edit] Response precautionary statements
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P301: IF SWALLOWED:
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P302: IF ON SKIN:
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P303: IF ON SKIN (or hair):
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P304: IF INHALED:
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P305: IF IN EYES:
•
P306: IF ON CLOTHING:
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P307: IF exposed:
•
P308: IF exposed or concerned:
•
P309: IF exposed or you feel unwell:
•
P310: Immediately call a POISON CENTER or doctor/physician
•
P311: Call a POISON CENTER or doctor/physician
•
P312: Call a POISON CENTER or doctor/physician if you feel unwell
•
P313: Get medical advice/attention
•
P314: Get Medical advice/attention if you feel unwell
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P315: Get immediate medical advice/attention
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P320: Specific treatment is urgent (see … on this label)
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P321: Specific treatment (see … on this label)
•
P322: Specific measures (see … on this label)
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P330: Rinse mouth
•
P331: Do NOT induce vomiting
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P332: If skin irritation occurs:
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P333: If skin irritation or a rash occurs:
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P334: Immerse in cool water/wrap in wet bandages
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P335: Brush off loose particles from skin
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P336: Thaw frosted parts with lukewarm water. Do not rub affected areas
•
P337: If eye irritation persists:
•
P338: Remove contact lenses if present and easy to do. continue rinsing
•
P340: Remove victim to fresh air and keep at rest in a position comfortable for breathing
•
P341: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing
•
P342: If experiencing respiratory symptoms:
•
P350: Gently wash with soap and water
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P351: Rinse continuously with water for several minutes
•
P352: Wash with soap and water
•
P353: Rinse skin with water/shower
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P360: Rinse immediately contaminated clothing and skin with plenty of water before removing clothes
•
P361: Remove/Take off immediately all contaminated clothing
•
P362: Take off contaminated clothing and wash before reuse
•
P363: Wash contaminated clothing before reuse
•
P370: In case of fire:
•
P371: In case of major fire and large quantities:
•
P372: Explosion risk in case of fire
•
P373: DO NOT fight fire when fire reaches explosives
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P374: Fight fire with normal precautions from a reasonable distance
•
P375: Fight fire remotely due to the risk of explosion
•
P376: Stop leak if safe to do so
•
P377: Leaking gas fire – do not extinguish unless leak can be stopped safely
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P378: Use … for extinction
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P380: Evacuate area
•
P381: Eliminate all ignition sources if safe to do so
•
<span class="abbr" style="color: blue; border-bottom: 1px dotted blue" title="Error in hazard statements">P391: Collect spillage
•
P301+310: IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician
•
P301+312: IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell
•
P301+330+331: IF SWALLOWED: Rinse mouth. Do NOT induce vomiting
•
P302+334: IF ON SKIN: Immerse in cool water/wrap in wet bandages
•
P302+350: IF ON SKIN: Gently wash with soap and water
•
P302+352: IF ON SKIN: Wash with soap and water
•
P303+361+353: IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Rinse skin with water/shower
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P304+312: IF INHALED: Call a POISON CENTER or doctor/physician if you feel unwell
•
P304+340: IF INHALED: Remove victim to fresh air and keep at rest in a position comfortable for breathing
•
P304+341: IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing
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P305+351+338: IF IN EYES: Rinse continuously with water for several minutes. Remove contact lenses if present and easy to do – continue rinsing
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P306+360: IF ON CLOTHING: Rinse immediately contaminated clothing and skin with plenty of water before removing clothes
•
P307+311: IF exposed: Call a POISON CENTER or doctor/physician
•
P308+313: IF exposed or concerned: Get medical advice/attention
•
P309+311: IF exposed or you feel unwell: Call a POISON CENTER or doctor/physician
•
P332+313: If skin irritation occurs: Get medical advice/attention
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P333+313: If skin irritation or a rash occurs: Get medical advice/attention
•
P335+334: Brush off loose particles from skin. Immerse in cool water/wrap in wet bandages
•
P337+313: Get medical advice/attention
•
P342+311: Call a POISON CENTER or doctor/physician
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P370+376: In case of fire: Stop leak if safe to do so
•
P370+378: In case of fire: Use … for extinction
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P370+380: In case of fire: Evacuate area
•
P370+380+375: In case of fire: Evacuate area. Fight fire remotely due to the risk of explosion
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P371+380+375: In case of major fire and large quantities: Evacuate area. Fight fire remotely due to the risk of explosion
[edit] Storage precautionary statements
•
P401: Store …
•
P402: Store in a dry place
•
P403: Store in a well ventilated place
•
P404: Store in a closed container
•
P405: Store locked up
•
P406: Store in a corrosive resistant/… container with a resistant inner liner
•
P407: Maintain air gap between stacks/pallets
•
P410: Protect from sunlight
•
P411: Store at temperatures not exceeding … °C/… °F
•
P412: Do not expose to temperatures exceeding 50 °C/122 °F
•
P420: Store away from other materials
•
P422: Store contents under …
•
P402+404: Store in a dry place. Store in a closed container
•
P403+233: Store in a well ventilated place. Keep container tightly closed
•
P403+235: Store in a well ventilated place. Keep cool
•
P410+403: Protect from sunlight. Store in a well ventilated place
•
P410+412: Protect from sunlight. Do not expose to temperatures exceeding 50 °C/122 °F
•
P411+235: Store at temperatures not exceeding … °C/… °F. Keep cool
•
P501 Dispose of contents/container to an approved waste disposal plant
Compared to the current EU system the most noticeable change are the pictograms (formerly: hazard symbols). While the most of the GHS pictograms have an
equivalent in the old system, the pictograms GHS 04, GHS 07 and GHS 08 are completely new.
The GHS System is built on 16 physical, 10 health and 3 environmental hazard classes and comprises the following communication elements:
9 Pictograms
Description
Pictogram
Hazard class and hazard category:
Exploding Bomb
GHS01
Unstable explosives
Explosives of Divisions 1.1, 1.2, 1.3, 1.4
Self reactive substances and mixtures, Types A,B
Organic peroxides, Types A,B
Flame
GHS02
Flammable gases, category 1
Flammable aerosols, categories 1,2
Flammable liquids, categories 1,2,3
Flammable solids, categories 1,2
Self-reactive substances and mixtures, Types B,C,D,E,F
Pyrophoric liquids, category 1
Pyrophoric solids, category 1
Self-heating substances and mixtures, categories 1,2
Substances and mixtures, which in contact with water,
emit flammable gases, categories 1,2,3
Organic peroxides, Types B,C,D,E,F
Flame Over Circle
GHS03
Oxidizing gases, category 1
Oxidizing liquids, categories 1,2,3
Gas Cylinder
GHS04
Gases under pressure:
- Compressed gases
- Liquefied gases
- Refrigerated liquefied gases
- Dissolved gases
Corrosion
GHS05
Corrosive to metals, category 1
Skin corrosion, categories 1A,1B,1C
Serious eye damage, category 1
Skull and Crossbones
GHS06
Acute toxicity (oral, dermal, inhalation), categories 1,2,3
Exclamation Mark
GHS07
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
Health Hazard
GHS08
Respiratory sensitization, category 1
Germ cell mutagenicity, categories 1A,1B,2
Carcinogenicity, categories 1A,1B,2
Reproductive toxicity, categories 1A,1B,2
Specific Target Organ Toxicity – Single exposure, categories 1,2
Specific Target Organ Toxicity – Repeated exposure, categories 1,2
Aspiration Hazard, category 1
Environment
GHS09
Hazardous to the aquatic environment
- Acute hazard, category1
- Chronic hazard, categories 1,2