Assessment of Largemouth Bass Micropterus salmoides Age, Growth, Gonad
Development and Diet in the Upper Barataria Estuary
A Thesis
Submitted to the Graduate Faculty of
Nicholls State University
In partial fulfillment of the requirements for the degree of
Master of Science
in
Marine and Environmental Biology
by
Bo Anthony Boudreaux
B.S., Nicholls State University, 2011
Spring 2013
CERTIFICATE
This is to certify that the thesis entitled “Assessment of Largemouth Bass
Micropterus salmoides Age, Growth, Gonad Development, and Diet in the Upper
Barataria Estuary” submitted for the award of Master of Science to Nicholls State
University is a record of authentic, original research conducted by Mr. Bo Anthony
Boudreaux under our supervision and guidance and that no part of this thesis has been
submitted for the award of any other degree, diploma, fellowship or other similar titles.
APPROVED:
Quenton Fontenot, Ph.D.
Associate Professor of
Biological Sciences
Committee Chair
Allyse Ferrara, Ph.D.
Associate Professor of
Biological Sciences
Committee Member
Gary LaFleur, Jr., Ph.D.
Associate Professor of
Biological Sciences
Committee Member
Aaron Pierce, Ph.D.
Associate Professor of
Biological Sciences
Committee Member
Tim Ruth
Biologist Manager
LDWF Inland Fisheries Dist. 8
Committee Member
SIGNATURE:
DATE:
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i
ABSTRACT
Lake Cataouatche and Lac des Allemands are located in the upper Barataria
Estuary. Lake Cataouatche is directly affected by a Mississippi River freshwater
diversion (Davis Pond), while Lac des Allemands receives no freshwater input from the
Mississippi River and is often affected by hypoxic conditions. Nutrients from the Davis
Pond diversion stimulated the growth of aquatic vegetation in and around Lake
Cataouatche that resulted in an apparent increase in the abundance and size of largemouth
bass in Lake Cataouatche in the late 2000s. To determine if there were population level
differences between largemouth bass from Lake Cataouatche and Lac des Allemands, I
compared relative abundance (catch per unit effort), gonadosomatic index (GSI), gonad
histology, diets, and age and growth of largemouth bass Micropterus salmoides collected
over one year. I also compared water quality in Lake Cataouatche to the water quality in
Lac des Allemands. Five-hundred and eighty-seven largemouth bass were collected for
this study by electrofishing (N=332 Cataouatche; N=255 des Allemands). Temperature
and dissolved oxygen were similar between the two lakes, but salinity was higher (never
> 1 ppt) in Lake Cataouatche than in Lac des Allemands. Hurricane Isaac (28 August
2012) caused hypoxic conditions in Lac des Allemands and areas surrounding Lake
Cataouatche resulting in large fish kills. There was no difference in largemouth bass
relative abundance for the spring and summer seasons between the lakes; however,
relative abundance was lower in the fall and winter seasons following Hurricane Isaac.
Largemouth bass in both lakes were more abundant in the presence of aquatic vegetation
( x = 3.91) than in the absence of aquatic vegetation ( x = 1.73). Largemouth bass GSI
was highest in March and February for both lakes, began to decrease in April, remained
ii
low in the summer and began to increase in October as expected. Male largemouth bass
from Lac des Allemands had higher GSIs ( x = 0.535, 0.11, 0.05, 0.02) than did males
from Lake Cataouatche in March, May, June and July. Females from Lac des Allemands
had higher GSIs ( x = 6.31, 0.48, 0.40) than females from Lake Cataouatche in March,
June and July. Based on GSI and gonad histology, I determined that largemouth bass in
both lakes spawned from the end of March to the beginning of April. Overall, diets of
largemouth bass from Lac des Allemands and Lake Cataouatche were different. The diets
of largemouth bass in Lake Cataouatche contained more fish in the spring than did
largemouth bass from either lake during the summer. Other major diet items included
insects, crawfish and crabs. Growth rates and abundance were similar in the two lakes.
Gonad development of largemouth bass from both lakes continued as expected after
Hurricane Isaac suggesting that surviving largemouth bass should spawn during the next
spawning season.
iii
ACKNOWLEDGEMENTS
First and foremost, I would like to thank my advisor, Dr. Quenton Fontenot. I
approached Dr. Fontenot in 2011 with ideas about this project, and although such a
project was not on his agenda at the time, he was excited and enthusiastic about the idea.
Within a couple weeks, he had found funding for the project, and we began to work on
our methods. I must also thank the Louisiana Department of Wildlife and Fisheries for
providing the funding for this project and especially Tim Ruth and Melissa Kaintz for
their assistance. I would also like to thank my committee members, Dr. Allyse Ferrara,
Dr. Gary LaFleur, and Dr. Aaron Pierce for their help and support throughout my project.
I would like to thank Nicholls State University and the Bayousphere Research
Laboratory for the use of their vehicles, boats and gear I used for this study. Thank you to
all the graduate students who helped me in the field and in the lab. I could not have
completed this research without them. I would like to especially thank Taylor Allgood, as
he accompanied me on nearly every trip and helped me load and unload that heavy
generator hundreds of times, and my fiancée Kellie Fangue for the endless hours she
helped me process fish in the lab. Special thanks go to Cheryl Crowder for processing my
histology slides and Zane LeBlanc for his training and assistance in the process of otolith
aging.
Lastly, I want to thank my parents, Marty and Thelma Boudreaux for their
continued love and support. Thank you dad for teaching me everything I know about
fishing and the great outdoors, and thank you mom for all that awesome cooking that has
kept me fueled for 23 years.
iv
TABLE OF CONTENTS
CERTIFICATE.....................................................................................................................i
ABSTRACT........................................................................................................................ii
ACKNOWLEDGEMENTS................................................................................................iv
TABLE OF CONTENTS.....................................................................................................v
LIST OF FIGURES............................................................................................................vi
LIST OFTABLES............................................................................................................viii
INTRODUCTION ..............................................................................................................1
METHODS........................................................................................................................12
RESULTS..........................................................................................................................20
DISCUSSION....................................................................................................................48
FUTURE RECOMMENDATIONS..................................................................................59
LITERATURE CITED .....................................................................................................61
APPENDIX I….................................................................................................................75
APPENDIX II ...................................................................................................................89
APPENDIX III ................................................................................................................103
APPENDIX IV ................................................................................................................106
BIOGRAPHICAL SKETCH...........................................................................................112
CURRICULUM VITAE .................................................................................................113
v
LIST OF FIGURES
Figure 1. The Barataria Estuary (outlined in black) in southeast Louisiana.......................7
Figure 2. Locations of study areas within the upper Barataria Estuary indicated by
stars…………………………………………………………………………..……9
Figure 3. Mean (± SE) temperature (A), dissolved oxygen (B) and salinity (C) for Lake
Cataouatche (solid line) and Lac des Allemands (dashed line) for each sample
date from 9 February 2012 to 24 January 2013………………………………….23
Figure 4. Images of dead fish in Lac des Allemands from 5 September 2012, one week
after Hurricane Isaac made landfall on 28 August 2012……………............……25
Figure 5. Mean (± SE) annual discharge of Davis Pond Diversion since opening in 2002.
.....................................................................……………..........………….……....26
Figure 6. Mean (± SE) catch per unit effort for largemouth bass collected in Lake
Cataouatche (black bars) and Lac des Allemands (white bars) during the spring
(11 April - 3 May 2012), summer (9 July – 18 July 2012), fall (9 October – 18
October 2012), and winter (23 January – 24 January 2013) sample periods…….28
Figure 7. CPUE of largemouth bass in the presence (white bars) and absence (black bars)
of floating vegetation (A) and rooted vegetation (B)…………………….........…30
Figure 8. Length-weight relationship for largemouth bass collected from Lac des
Allemands (black squares; R2 = 0.8413) and Lake Cataouatche (white diamonds;
R2 = 0.8368) from 9 February 2012 to 24 January 2013………………………...31
Figure 9. Mean (± SE) total length for female and male largemouth bass collected in
Lake Cataouatche (black bars) and Lac des Allemands (white bars) from 9
February 2012 to 24 January 2013…………………………………………….....32
Figure 10. Mean (± SE) TL at each age for female (black) and male (dotted) largemouth
bass collected from Lake Cataouatche and female (white) and male (hatched)
largemouth bass collected from Lac des Allemands……………………………..34
Figure 11. Total number of female (black) and male (dotted) largemouth bass collected
from Lake Cataouatche and female (white) and male (hatched) largemouth bass
collected from Lac des Allemands for each age class………...……………........35
Figure 12. A von Bertalanffy growth curve, maximum theoretical total length (L∞), von
Bertalanffy growth coefficient (k), and time when total length would theoretically
equal zero (to) for largemouth bass collected in the upper Barataria Estuary from 9
February 2012 to 24 January 2013.....................................................…………....36
Figure 13. Percent of largemouth bass diet by diet category for fish collected from Lake
Cataouatche (Cat) and Lac des Allemands (dA) for the spring and summer
seasons……………..………………………………………………………….....38
vi
Figure 14. Mean (± SE) gonadosomatic index (GSI) for female (A) and male (B)
largemouth bass collected from Lac des Allemands (dashed line) and Lake
Cataouatche (solid line) from 9 February 2012 to 24 January 2013……………..39
Figure 15. Mean (± SE) monthly gonadosomatic index (GSI) for female (A) and male
(B) largemouth bass collected from Lac des Allemands (open bars) and Lake
Cataouatche (dark bars) from 9 February 2012 to 24 January 2013……………..41
Figure 16. Percent of “developing” (open bar), “spawning capable” (black bar),
“regressing” (light grey bar), and “regenerating” (dark grey bar) gonad
developmental stages of female largemouth bass collected 9 February 2012 to 24
January 2013, from Lac des Allemands (A) and Lake Cataouatche (B)………...42
Figure 17. Histological section from the ovary of a spawning capable female largemouth
bass (TL = 560 m) collected on 16 February 2012, in Lake Cataouatche…...…..43
Figure 18. Histological section from the ovary of a regressing female largemouth bass
(TL = 345 mm) collected on 18 April 2012, in Lac des Allemands……………..44
Figure 19. Histological section from the ovary of a regenerating female largemouth bass
(TL = 476 mm) collected on 14 August 2012, in Lac des Allemands…………...45
Figure 20. Histological section from the ovary of a developing female largemouth bass
(TL = 264 mm) collected on 20 November 2012, in Lake Cataouatche………...46
Figure 21. Histological section from the ovary of an immature female largemouth bass
(TL = 176 mm) collected on 27 April 2012, in Lac des Allemands……………..47
vii
LIST OF TABLES
Table 1. Processing procedure for histological preparation of largemouth bass gonad
samples……………………………………………………………………..…….16
Table 2. Staining procedure for histological preparation of largemouth bass gonad
samples……………………………………………………………………….…..17
Table 3. Description of reproductive classification system for female fishes according to
histological characteristics of gonads (modified from Brown-Peterson et al.
2011)………………………..……………………………………………………18
Table 4. Number of each species collected from Lake Cataouatche (Cat) and Lac des
Allemands (des) during the spring, summer, fall and winter sample season…….21
Table 5. Combined Mean (± SE) temperature (C), salinity (ppt) and dissolved oxygen
(mg/L) for Lake Cataouatche and Lac des Allemands ......................…………...24
Table 6. List of all floating and rooted vegetation species observed............................... 29
Table 7. Mean (± SE) number of diet items in largemouth bass during spring and summer
sampling…………………..……………………………………...............………37
viii
INTRODUCTION
Largemouth bass Micropterus salmoides are members of the Centrarchidae family
and are native to North America. Largemouth bass have a slightly compressed body, a
large sloping mouth, and are dark to light green. Largemouth bass originally inhabited
waters throughout eastern-central North America (Scott and Crossman 1973), but have
been introduced throughout most of North America (reviewed in Brown et al. 2009).
Largemouth bass inhabit swamps, ponds, lakes, reservoirs, creeks, estuaries, large rivers
and floodplains, but prefer the shoreline of lacustrine environments and are rarely found
in waters greater than 6 m deep (Scott and Crossman 1973). Largemouth bass feed
mostly by sight but can use odors and vibrations to locate prey (Scott and Crossman
1973). Largemouth bass fry mostly consume insects, but adults are mainly piscivorous.
The potential longevity of largemouth bass is 23 years, but individuals rarely reach 15
years in the wild (reviewed in Brown et al. 2009). Largemouth bass are the most popular
sportfish in North America and have become a management priority for many state
fisheries agencies (Fries 2010).
The two sub-species of largemouth bass are the northern and Florida strains
(Bailey and Hubbs 1949). Although Florida strain largemouth bass can grow faster and
larger than the northern strain in southern latitudes (Maceina and Murphy 1992), growth
and survival of Florida strain largemouth bass may be lower than northern strain
largemouth bass in northern latitudes (Clugston 1964; Philipp and Whitt 1991). Growth
rates of largemouth bass vary greatly across their range with southern populations having
the highest growth rates (reviewed in Brown et al. 2009). The difference in growth rates
is likely due to a longer growing season and warmer temperatures in southern latitudes
1
(Clugston 1964; Coutant and Deangelis 1983). Viosca (1943) reported a growth rate of
226 g/month for a 7 month period for one largemouth bass in Louisiana, but the average
growth of adult largemouth bass in the mid-east United States is approximately 450
g/year (Stuber et al. 1982). Largemouth bass can gain approximately 500 g in body
weight for every 1800 g of food they ingest (Scott and Crossman 1973), but growth can
be influenced by environmental factors such as temperature and salinity (Weatherley
1990).
Fish growth rates generally increase with temperature, but growth rates will
decrease if temperature reaches an upper critical level. Largemouth bass do not grow in
temperatures less than 15˚C and reach maximum growth rates between 24 and 30˚C
(Venables et al. 1978). Largemouth bass growth rates decrease in temperatures above
30˚C and growth ceases at temperatures greater than 36˚C (Stuber et al. 1982).
Largemouth bass generally spawn in the spring when temperatures reach 15.6- 21˚C
(Clugston 1964; Allen and Romero 1975), but spawning has been documented in
temperatures ranging from 13 – 26˚C (Kelley 1968). Largemouth bass embryos cannot
tolerate temperatures below 10˚C or above 30˚C (Kramer and Smith 1962).
Largemouth bass are more tolerant of low dissolved oxygen (DO) levels than are
smallmouth bass Micropterus dolomieu and bluegill sunfish Lepomis macrochirus (Moss
and Scott 1961; Scott and Crossman 1973). Largemouth bass are generally found in
waters with dissolved oxygen levels above 3 mg/L (reviewed in Brown et al. 2009) and
cannot tolerate DO levels below 1 mg/L (Stuber et al. 1982). Although largemouth bass
larvae cannot tolerate dissolved oxygen levels at 1.0 mg/L at 20˚C, the lethal DO limit
increases with temperature (Spoor 1997). Moss and Scott (1961) found that largemouth
2
bass are more tolerant of low DO levels (≤2.0 mg/L) when levels decrease slowly (0.3
mg/L/hour). Because largemouth bass usually remain within a 2 hectare area (Winter
1977; Fish and Savitz 1983), largemouth bass may be susceptible to abrupt decreases in
DO.
Hypoxic conditions (DO ≤ 2.0 mg/L) make habitats intolerable to many fish
species (Fontenot et al. 2001; Killgore and Hoover 2001). Hypoxic conditions can occur
in the water column when bacteria deplete the available oxygen by decomposing organic
matter. In the upper Barataria Estuary, hypoxic conditions usually coincide with locally
heavy rainfall and can last from a few days to several weeks (Estay 2007; Eddlemon
2009). The wind energy and heavy rainfall associated with hurricanes can increase the
amount of organic matter in the local water column, which can lead to increased rates of
microbial decomposition and hypoxic conditions (van Vrancken and O'Connell 2010).
South Louisiana is frequently impacted by hurricanes, and fish kills often occur because
of sudden declines in DO levels (Tilmant et al. 1994; Stevens et al. 2006).
Largemouth bass are a freshwater species that can be abundant in estuarine
habitats and can be common in salinities ranging from 0.5-5.0 ppt (reviewed in Brown et
al. 2009). Although adults can survive in water up to 24 ppt (Moyle 1976; Peer et al.
2006), embryonic development is impaired at 1.5 ppt, and embryos cannot survive
salinities above 10.5 ppt. Growth of largemouth bass fry decreases with increased salinity
and ceases at 6 ppt (Tebo and McCoy 1964). Storm surges associated with hurricanes
typically push saltwater inland into freshwater habitats. Sudden changes in salinity may
cause stress and may be lethal to some fish. Because hurricanes generally occur after the
largemouth bass spawning season, it is unlikely that hurricanes affect the spawning
3
success of largemouth bass. However, large fish kills associated with hurricanes may
reduce reproductive output in years following a hurricane.
Largemouth bass are top predators and consume a variety of organisms. However,
there is a noticeable shift in the diet as largemouth bass mature. Largemouth bass larvae
consume mostly zooplankton. Juvenile largemouth bass consume insects but shift to a
more piscivorous diet once reaching approximately 60 mm TL (reviewed in Brown et al.
2009). Mature largemouth bass consume mostly fish and crustaceans (Stein 1970). Adult
largemouth bass feed mainly on other fish (reviewed in Brown et el. 2009), but
largemouth bass diets can vary depending on the amount and type of vegetation available
(Dibble and Harrel 1997). The upper Barataria Estuary is a highly productive estuarine
system and should provide ample prey for largemouth bass of all sizes.
Otoliths, scales, spines, and fin rays can be used to estimate fish age (Welch et al.
1993; Soupir et al. 1997; Maceina and Sammons 2006), but otoliths are the most accurate
structure for estimating largemouth bass age (Besler 1999). Age data are required to
determine annual growth rates, differences in growth rates between the sexes or different
populations, age of maturity, and year class strength. Environmental factors such as
temperature, photoperiod and flood duration play a key role in the reproduction of many
fishes (de Vlaming 1972; Bayley 1995) and often cause fish populations to be dominated
by one or two year classes (reviewed in Fox 2010). Such is the case for bluegill
(Santucci and Wahl 2003), yellow bass (Fox 2010) and largemouth bass (Kramer and
Smith 1962). Less predictable occurrences such as storms or droughts may also produce
dominant year classes.
4
Largemouth bass can mature in one year if growth reaches 255 mm TL, but
maturation likely occurs later in northern populations (reviewed in Tidwell 2000).
Duration and timing of largemouth bass spawning varies depending on latitude. Spengler
(2010) found that largemouth bass pairs began displaying courtship behaviors when
water temperatures reached 14˚C. Peak spawning of largemouth bass occurs in water
temperatures from 15.6 to 21˚C (Clugston 1964; Allan and Romero 1975), suggesting
that southern largemouth bass populations spawn earlier than northern populations.
Brown et al. (2009) provide a comprehensive review of largemouth bass
spawning behavior and report that largemouth bass are batch spawners and may spawn
several times over a six week period. A male largemouth bass begins the spawning
process by clearing a disk shaped area in the substrate with his tail. A gravid female
drops a portion of her eggs in the nest, and the male deposits sperm over the eggs. A
female may spawn multiple times within a single spawning season with one or several
males. Males guard the nest until the larvae hatch in 3-5 days. Larvae swarm together
forming tight brood groups, and the male may stay with the brood for several weeks until
the fry disperse.
The gonadosomatic index (GSI) is used to determine spawning periods of fish by
tracking changes in gonad weight in relation to total fish weight (Nieland and Wilson
1993; Jons and Miranda 1997). GSI is highest just before a fish spawns, and decreases as
gametes are released during spawning. GSI remains low throughout the non-spawning
season when gonads are regenerating. GSI increases as eggs and sperm mature. Gonad
histology is used to classify individuals into specific reproductive categories. Through
this method, fish can be classified as “immature” (not capable of spawning),
5
“developing” (gonads developing but not capable of spawning), “spawning capable”
(able to spawn or active or recent spawning), “regressing” (post-spawning), or
“regenerating” (post-spawning and recovering; Brown-Peterson et al. 2011). Gonad
histology can be used in conjunction with GSI to precisely define the active spawning
period.
Many fishes of large rivers evolved feeding and spawning strategies that coincide
with the annual floodpulse (Junk et al. 1989). The Mississippi River is the third longest
river in the world, has the fourth largest drainage area and the sixth largest discharge,
(Turner and Rabalais 1994), and drains approximately 41% of the continental United
States. Since the construction of levees along the Mississippi River, over 90% of the
river’s floodplains have been disconnected from the river (Bayley 1995). Partial
reconnection of Mississippi River floodplains may provide a self-sustaining potential for
recreational and commercial fisheries and flood control (Bayley 1991), and may return
aquatic habitats to pre-industrial levels of biodiversity. Mississippi River diversions may
be a logical method for restoring pre-industrial Mississippi River floodplains by bringing
fresh water, sediments and nutrients into swamps and marshes that have been separated
from the river (Martin 2002).
The Barataria Estuary (Figure 1) is the southern-most western floodplain of the
Mississippi River and was historically inundated by the river’s annual spring floodpulse
(Bahr and Hebrard 1976). Construction of flood protection levees and the resulting
6
N
Figure 1. The Barataria Estuary (outlined in black) in southeast Louisiana. Bar = 100 km.
7
disconnection of river distributaries prevent the upper Barataria Estuary from receiving
the seasonal Mississippi River floodpulse (Sklar and Conner 1979; Swenson 2006; Inoue
et al. 2008). Unintended consequences of channelization of the Mississippi River are
increased subsidence rates and saltwater intrusion into historically freshwater areas of the
Barataria Estuary (Inoue et al. 2008). To reduce coastal land loss and saltwater intrusion,
the Davis Pond Freshwater Diversion Structure (Davis Pond) was constructed to
reintroduce Mississippi River water into the Barataria Estuary (Swenson et al. 2006).
Davis Pond was opened in 2002 and has the capacity to divert up to 302 m3/s
(10,650 f3/s) of water into the Barataria Estuary. One of the most obvious impacts of the
opening of the Davis Pond diversion was increased growth of submerged aquatic
vegetation in Lake Cataouatche (Poirrier et al. 2010). The release of Mississippi River
water through Davis Pond has stimulated vegetative and fisheries production within the
Lake Cataouatche area of the Barataria Estuary (Poirrier et al. 2010). Anecdotal reports
by local anglers indicated that catch rates and size of largemouth bass increased in the
Lake Cataouatche area as a result of increased vegetation. Because availability of
submerged aquatic vegetation is correlated to largemouth bass productivity (Durocher et
al. 1984; Hoyer and Canfield 1996; Brown and Maceina 2002), increased growth of
aquatic vegetation in Lake Cataouatche may benefit the local largemouth bass population.
In contrast, Lac des Allemands is located “upstream” of the diversion and has not
experienced a similar increase in vegetation.
Historically, Lac des Allemands (Figure 2) received an annual floodpulse with
Mississippi River water entering the western side of the lake through Bayou Chevreuil
8
Figure 2. Locations of Lac des Allemands and Lake Cataouatche within the Upper
Barataria Estuary indicated by stars. Bar = 20 km.
9
D
(Fox 2010). The importance of such floodpulses to local fish populations is well
documented (Junk 1999; Schramm and Eggleton 2006), but fish in Lac des Allemands
are not currently exposed to an influx of fresh, oxygenated Mississippi River water.
Hypoxic conditions, such as those seen in and around Lac des Allemands create
unsuitable habitat and can cause stress or death in local fish populations (Killgore and
Hoover 2001; Arend et al. 2011).
Davis Pond has added nutrients and sediment to Lake Cataouatche and may have
caused increases in largemouth bass abundance and growth, and may affect the diet and
gonad development of largemouth bass. If Davis Pond has had a direct or indirect impact
on the Lake Cataouatche largemouth bass population, then I did not expect to see the
same abundance, growth, diet, and gonad histology for the largemouth bass population in
Lac des Allemands. The goal of this study was to compare characteristics of the
largemouth bass populations in Lac des Allemands and Lake Cataouatche. The specific
objectives of this study were to
1. Determine differences in temperature, DO and salinity between Lac des
Allemands and Lake Cataouatche.
2. Determine differences in relative abundance, mean size, and the length-weight
relationship of largemouth bass between Lac des Allemands and Lake
Cataouatche.
3. Determine differences in the relative abundance of largemouth bass in the
presence of aquatic vegetation to the relative abundance of largemouth bass in
the absence of aquatic vegetation.
10
4. Determine differences in the size at age and growth rates between male and
female largemouth collected from Lac des Allemands and Lake Cataouatche.
5. Determine differences in seasonal stomach contents of largemouth bass from
Lac des Allemands and Lake Cataouatche.
6. Determine differences in the monthly gonadosomatic index for male and
female largemouth bass between Lac des Allemands and Lake Cataouatche.
7. Determine differences in the monthly frequency of each reproductive stage
between female largemouth bass collected from Lac des Allemands and Lake
Cataouatche.
11
METHODS
To compare the largemouth bass populations in Lac des Allemands and Lake
Cataouatche, largemouth bass were collected by electrofishing in both lakes from 9
February 2012 to 25 January 2013. A Smith-Root 7.5 Generator Powered Pulsator (GPP)
was used to collect all samples. Pulsed Direct Current (DC), 60 pulses/second, was used
at various percentages of maximum range to maintain 6-8 amperes of current. The
shoreline of each lake was divided into 1,000 m sections and a random number generator
was used to select shoreline sections for each seasonal sampling period.
I used two sampling protocols for this project (seasonal and monthly). For the
first (seasonal) protocol, 12 random sites were sampled each season (spring, summer, fall,
winter) in each lake to collect relative abundance, stomach contents, age, size, and gonad
samples from largemouth bass. All collections were made during one calendar month
within each season (spring = April, summer = July, fall = October, winter = January). For
each season, each random site was sampled in each lake by electrofishing for 600
seconds. Collected fish were immediately placed in an aerated livewell. At the conclusion
of each 600 second sample, all largemouth bass were measured (TL; mm). Up to 10
individuals per 25 mm (1 inch) size group were sacrificed to collect weight (g) stomach
content, gonad and otolith samples (Louisiana Department of Wildlife and Fisheries
(LDWF) protocol). All sacrificed largemouth bass were placed on ice immediately after
being measured. All other fish collected during seasonal sampling were released after
species was identified.
For the second (monthly) protocol, up to 15 female and 10 male largemouth bass
were collected from non-random sites during the months not included in the seasonal
12
sampling to collect monthly age, size, and gonad samples. Fish kills associated with
Hurricane Isaac (landfall on 28 August 2012) severely reduced the number of largemouth
bass, and adequate sample sizes were not always collected after the hurricane. Monthly
sampling occurred either along the shoreline of each lake or along the shoreline in areas
directly connected to each lake. Fish were measured (TL; mm), weighed (g), otoliths
were removed and gonads were removed and weighed (g). Catch per unit effort (CPUE)
was calculated for each sampling season as the mean number of largemouth bass caught
per 600 seconds of electrofishing.
Relative Abundance
Each of the 12 sections sampled seasonally per lake represent an experimental
unit (N = 12 per lake per season). Relative abundance (CPUE) for each section was
calculated as the number of largemouth bass collected per 600 seconds of electrofishing.
The largemouth bass relative abundance was compared between the lakes for each season
using analysis of variance.
Age and Growth
Sagittal otoliths were removed from all sacrificed largemouth bass for age and
growth assessment (following LDWF protocol). All otoliths were stored dry in labeled
vials for 5 days. After the fifth day, glycerin was added to the vials. Otoliths were aged in
whole view with a dissecting microscope by two separate readers. Discrepancies were
discussed until both readers agreed on an age. Each otolith was assigned an annuli count
and an edge code. Edge codes describe the developmental stage of the annulus formation
on the outer edge of the otolith. All otoliths were assigned two numbers. The first number
13
corresponded to the number of annuli on the otolith. The second number (the edge code)
refers to the amount of growth on each otolith past the last annulus and represented the
amount of time since the last annulus was formed. An edge code of 1 designates the least
amount of growth and 4 the most; therefore, a fish aged as a 3/1 is 3 years old but is
almost one year younger than a 3 year old fish aged as a 3/4. All otoliths scored as a 3/3
or older were sectioned and mounted to a microscope slide for viewing at higher
magnification with a compound microscope. Otoliths aged 3/3 and older were embedded
in a two part araldite epoxy, and were sectioned after the epoxy hardened using an
IsoMet® 1000 Precision Saw. Three transverse sections of each otolith were cut and the
two sections with the most visible annuli were fastened to a slide using Loctite® 349.
The other section was thrown away. After curing, the age of each otolith was
independently determined by two readers, and a final age was agreed on. Using ANOVA,
mean size at age was compared for male and female largemouth bass collected from each
lake. Because there was no differences between sexes or lakes, data for the two lakes and
both sexes were pooled to create an overall von Bertalanffy growth curve for the upper
Barataria Estuary. To produce a more accurate von Bertalanffy growth curve, 70
additional young-of-the-year largemouth bass were collected, measured, and released. All
fish that were aged were given a biological age based on otolith age, collection date, and
an assumed April 1st birthday (LDWF Protocol).
Histology
Gonads were removed from all sacrificed largemouth bass to calculate
gonadosomatic index (GSI) and female ovaries were sectioned for histological analysis.
Five mm sections were cut from the center of the ovaries and were placed in 10% neutral
14
buffered formalin. Ovary samples were placed in labeled tissue cassettes, stored in 75%
reagent alcohol and sent to Crowder Histology (Baton Rouge, Louisiana) for processing
(Tables 1 and 2). Histological analyses followed the methods of Brown-Peterson et al.
(2011). Based on development of gonads, individuals were classified as immature,
developing, spawning capable, regressing, or regenerating (Table 3). The monthly
frequency of each reproductive category was compared between the lakes.
Diet
Stomachs were removed from each bass collected during seasonal sampling,
placed in individually labeled cotton bags (Hubco, Hutchinson, KS) and preserved in
75% isopropyl alcohol until processed. Stomachs were cut open with scissors and
contents were removed and identified to the lowest possible taxon. Some stomach
contents could not be identified below the class taxon due to advanced digestion.
Therefore, stomach content analyses were based on the groupings fish, shrimp, crab,
crayfish, and insect. Plant matter was classified as detritus, and anything that could not be
identified was classified as unidentified.
Multivariate analysis of variance (Wilk’s Lambda) was used to determine if
seasonal diets differed within each lake and between lakes for the spring and summer
seasons. I did not collect enough largemouth bass in the fall or winter sample to make
comparisons between each lake. Analysis of variance was used to compare seasonal
differences for each diet grouping. Chi square analysis was used to compare the percent
of empty stomachs between lakes.
15
Table 1. Processing procedure for histological preparation of largemouth bass gonad
samples. Alcohol – Ethyl alcohol, 100% anhydrous Pharmco-AAPER, Shelbyville, KY;
Xylene – Macron Fine Chemicals, Center Valley, PA; Paraffin – Paraplast Tissue
Embedding Medium, McCormick Scientific (Leica Microsystems).
Reagent
Time
Alcohol, 70%
Alcohol, 80%
Alcohol, 95%
Alcohol, 95%
Alcohol, 100%
Alcohol, 100%
Alcohol, 100%
Xylene
Xylene
Xylene
Paraffin
Paraffin
Paraffin
Until Start
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes
45 minutes 60˚C
45 minutes 60˚C
45 minutes 60˚C
16
Table 2. Staining procedure for histological preparation of largemouth bass gonad
samples. Alcohol – Ethyl alcohol, 100%, anhydrous, Pharmco-AAPER, Shelbyville, KY;
Xylene – histologic, Macron Fine Chemicals, Center Valley, PA; Hematoxylin 560 –
Surgipath SelecTech, Leica Microsystems; Eosin-Phoxine 515 – Surgipath SelecTech,
Leica Microsystems; Blue Buffer – Surgipath SelecTech, Leica Microsystems; Define
MX- aq – Surgipath SelecTech, Leica Microsystems; Acrymount Mounting Medium –
Anapath, StatLab Medical Products, McKinney, TX.
Reagent
Xylene
Xylene
Xylene
Alcohol, 100%
Alcohol, 100%
Alcohol, 100%
Water, running
Hematoxin
Water, running
Acid Rinse
Water, running
Blueing
Water, running
Eosin-phloxine
Alcohol, 100%
Alcohol, 100%
Xylene
Xylene
Xylene
Xylene
Time
5 minutes
5 minutes
5 minutes
5 minutes
5 minutes
5 minutes
2 minutes
3 minutes
2 minutes
30 seconds
1 minute
1 minute
2 minutes
10 dips
10 dips
1 minute
10 dips
10 dips
10 dips
until coverslipped
17
Table 3. Description of reproductive classification system for female fishes according to
histological characteristics of gonads (as modified from Brown-Peterson et al. 2011).
PGO—primary growth oocytes; CAO—cortical alveolar oocytes; CA—cortical alveoli;
VTG1—early vitellogenic oocytes; VTG2— mid vitellogenic oocytes; VTG3—late
vitellogenic oocytes POF—post-ovulatory follicles; OM—oocyte maturation.
Phase
Description
Immature (never spawned)
Small ovaries, often clear, blood vessels indistinct.
Only oogonia and PGO present. No atresia or
muscle bundles. Thin ovarian wall and little space
between oocytes.
Developing (ovaries
beginning to develop,
but not ready to spawn)
Enlarging ovaries, blood vessels becoming more
distinct. PG, CA, VTG1, and VTG2 oocytes
present. No evidence of POFs or Vtg3 oocytes.
Some atresia can be present.
Spawning capable (fish are
developmentally and
physiologically able to
spawn in this cycle)
Large ovaries, blood vessels prominent. Individual
oocytes visible macroscopically. VTG3 oocytes
present or POFs present in batch spawners. Atresia
of vitellogenic and/or hydrated oocytes may be
present. Early stages of OM can be present.
Regressing (cessation of
spawning)
Flaccid ovaries, blood vessels prominent. Atresia
(any stage) and POFs present. Some CA and/or
vitellogenic (VTG1, VTG2) oocytes present.
Regenerating (sexually
mature, reproductively
inactive)
Small ovaries, blood vessels reduced but present.
Only oogonia and PGO present. Muscle bundles,
enlarged blood vessels, thick ovarian wall and/or
gamma/delta atresia or old, degenerating POFs
may be present.
18
Vegetation
For each 1000 m electrofishing section, five transects (1 m wide x 10 m long)
were sampled at the conclusion of electrofishing to estimate aquatic vegetation species
composition and coverage. A random number generator was used to select the locations
of the transects within the electrofishing section by assigning five distances measured by
GPS, originating from the terminating end of the 600 seconds electrofishing reach,
extending toward the 0 seconds electrofishing point. The transect lines were constructed
using a 10 m long floating rope marked in1 m increments and anchored by PVC pipe on
each end. A floating 0.5 m2 (1 m x 0.5m) quadrat was placed on both sides of the line,
and the aerial coverage for all species visible within the quadrats was estimated. Plants
were classified as rooted or floating, and presence or absence for each transect was noted.
To determine if the presence of aquatic vegetation affected relative abundance of
largemouth bass, sites that had aquatic vegetation present were compared to sites where
aquatic vegetation was absent.
Water Quality
Dissolved oxygen (mg/L), salinity (ppt), and temperature (°C) were measured in
the middle of the water column at the outside edge of the vegetation following
electrofishing and prior to quantifying the vegetation. ANOVA was used to compare the
mean value of temperature, dissolved oxygen and salinity between Lac des Allemands
and Lake Cataouatche. Data from USGS was used to gather mean yearly discharge data
from Davis Pond from 2003 to 2012.
19
RESULTS
A total of 587 largemouth bass (N=332 Cataouatche; N=255 des Allemands) were
collected from 9 February 2012 to 25 January 2013. Of the 587 largemouth bass
collected, 564 (N = 312 female; N = 252 male) were sacrificed to collect otoliths, gonads
and stomachs. Only length and weight were recorded for the other 23 bass. In addition to
largemouth bass, 35 other species were collected (Table 4).
Temperature peaked in both lakes in July and began to decrease in August (Figure
3), and there was no difference in temperature between the two lakes (F=0.031, 32;
P=0.8639; Table 5). The eye of Hurricane Isaac made landfall on 28 August 2012 at
Venice in south Louisiana and high winds and heavy precipitation directly affected the
study area. Although the mean dissolved oxygen level for Lac des Allemands (7.14 ±
0.52) was similar to Lake Cataouatche (9.15 ± 0.52; F=1.741, 32; P=0.1966; Table 5), Lac
des Allemands became hypoxic within days after Hurricane Isaac made landfall. I
observed fish kills (Figure 4) in Lac des Allemands and areas surrounding Lake
Cataouatche as a result of hypoxic conditions after Hurricane Isaac (Figure 3). Although
much of Lake Cataouatche was hypoxic, I observed areas of DO > 2.0 mg/L, which
indicates that refuge areas were available. Dissolved oxygen levels in Lac des Allemands
returned to levels above hypoxic within one month after Hurricane Isaac. Although
salinity was slightly higher in Lake Cataouatche than Lac des Allemands (F=31.961, 32;
P=<0.0001; Table 5), neither lake exceeded 1.0 ppt and both were considered freshwater
systems for the duration of this study. Mean annual flow rates of the Davis Pond
Diversion have fluctuated since the diversion opened in 2002 (Figure 5). The four
20
Table 4. Number of each species collected from Lake Cataouatche (Cat) and Lac des
Allemands (des) during the spring, summer, fall and winter sampling season. Bold
species names indicate those that were not collected in both lakes.
Species
Common
name
Mugil cephalus
Striped
mulllet
Lepisosteous oculatus
Micropterus
salmoides
Lepomis macrochirus
Spring
Cat des
Summer
Cat des
Fall
Cat des
Winter
Cat des
112
202
44
155
56
25
13
27
Spotted gar
Largemouth
bass
80
15
136
30
36
22
28
24
75
68
70
79
4
3
1
2
Bluegill
Channel
catfish
74
95
83
130
4
26
1
6
63
53
17
4
5
5
1
8
Black crappie
Freshwater
drum
Blue catfish
Redear
sunfish
56
85
8
62
2
7
2
0
30
19
0
14
142
16
0
0
38
10
0
0
8
7
0
5
15
9
8
28
1
2
0
0
Sciaenops ocellatus
Dorosoma
cepedianum
Cyprinus carpio
Redfish
13
0
35
0
69
0
3
0
Gizzard shad
Common carp
12
11
17
27
99
2
18
6
16
5
4
16
1
3
0
31
Morone chrysops
White bass
Smallmouth
buffalo
American eel
Redspotted
sunfish
Atlantic
needlefish
Yellow
bullhead
Inland
silverside
Skipjack
herring
Bigmouth
buffalo
10
0
11
0
10
0
0
0
8
7
3
4
6
3
0
0
2
5
2
1
2
0
0
1
5
3
0
5
0
0
0
0
5
0
0
1
1
0
0
0
4
0
0
6
0
1
0
0
4
3
2
0
0
0
0
0
3
3
0
0
0
0
1
0
3
1
2
2
1
0
1
0
Warmouth
Southern
flounder
3
1
0
3
0
0
0
0
3
0
3
0
1
0
2
0
Ictalurus punctatus
Pomoxis
nigromaculatus
Aplodinotus
grunniens
Ictalurus furcatas
Lepomis microlophus
Ictiobus bubalus
Anguilla rostrata
Lepomis miniatus
Strongylura marina
Ameiurus natalis
Menidia beryllina
Alosa chrysochloris
Ictiobus cypinellus
Chaenobryttus
gulosus
Paralichthys
lethostigma
21
Amia calva
Bowfin
Gulf
menhaden
2
2
1
1
1
2
2
1
2
0
0
0
0
0
0
0
Sheepshead
Black
Buffalo
1
0
1
0
4
0
1
0
1
0
0
0
1
0
1
0
1
0
8
0
3
0
0
0
1
0
0
0
0
0
0
0
1
1
0
3
1
0
1
1
0
0
7
0
0
8
5
22
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
Lepomis megalotis
Spot
Bantam
sunfish
Flathead
catfish
Threadfin
shad
Ladyfish
Orangespotted
sunfish
Longear
sunfish
0
1
0
1
0
0
0
0
Morone
mississippiensis
Notemigonus
crysoleucas
Yellow bass
Golden
shiner
0
0
5
0
0
0
0
0
0
0
0
7
0
1
0
0
Brevoortia patronus
Archosargus
probatocephalus
Ictiobus niger
Leiostomus
xanthurus
Lepomis
symmetricus
Pylodictis olivaris
Dorosoma petenense
Elops saurus
Lepomis humilis
22
30
A
Temperature (C)
24
18
12
6
0
2/1/12
Dissolved Oxygen (mg/L)
18
4/16/12
6/30/12
9/13/12
11/27/12
4/16/12
6/30/12
9/13/12
11/27/12
4/16/12
6/30/12
9/13/12
11/27/12
2/10/13
B
15
12
9
6
3
0
2/1/12
Salinity (ppt)
0.8
2/10/13
C
0.6
0.4
0.2
0
2/1/12
2/10/13
Date
Figure 3. Mean (± SE) temperature (A), dissolved oxygen (B) and salinity (C) for Lake
Cataouatche (solid line) and Lac des Allemands (dashed line) for each sample date from
9 February 2012 to 24 January 2013.
23
Table 5. Mean (± SE) temperature (C), salinity (ppt) and dissolved oxygen (mg/L) for
Lake Cataouatche and Lac des Allemands for all samples combined. The asterisk
indicates a difference between the lakes.
Lake
Temperature
Salinity*
Dissolved Oxygen
Cataouatche
20.0 ± 1.64
0.3 ± 0.04
9.1 ± 0.95
des Allemands
19.7 ± 1.23
0.1 ± 0.01
7.4 ± 0.91
24
Figure 4. Images of dead fish in Lac des Allemands from 5 September 2012, one week
after Hurricane Isaac made landfall on 28 August 2012.
25
120
A
Mean Discharge (m/sec3)
100
A
B
80
60
ABC
40
20
BC
C
C
ABC
BC
B
C
C
0
2003
2004
2005
2006
2007 2008
Year
2009
2010
2011
2012
Figure 5. Mean (± SE) annual discharge of Davis Pond Diversion since opening in 2002.
Means with similar letters are not different. December 2012 datum is subject to change.
Datum from 2002 is excluded because Davis Pond was only run for one day in 2002.
26
highest mean yearly flow rates were in the four years prior to this project.
Largemouth bass CPUE was similar for the spring and summer seasonal sampling
for both lakes (Figure 6). Although, CPUE was lower (F=13.787, 88; P=<0.0001) for the
fall and winter than the spring and summer for each lake, there was no difference
between the lakes for any season (Figure 6).
In all seasons combined, 57 sections sampled contained floating vegetation (N=35
pre-Isaac; 22 post-Isaac), 32 contained rooted vegetation (N=30 pre-Isaac; 10 post-Isaac)
and 38 contained no vegetation. Vegetation species encountered are listed in Table 6.
Largemouth bass CPUE was greater in the presence of rooted vegetation than in the
absence of vegetation before (F=4.281, 46; P=0.0443) and after (F=4.561, 46; P=0.0381)
Hurricane Isaac (Figure 7). CPUE was also greater in the presence of floating vegetation
than in the absence of vegetation before (F=5.021, 46; P=0.0299) and after (F=4.121, 46;
P=0.0482) the hurricane (Figure 7).
Total length of largemouth bass collected during this study ranged from 95 mm to
560 mm and weight ranged from 12 g to 3,662 g. There was no difference in the lengthweight relationship of largemouth bass between Lake Cataouatche and Lac des
Allemands (t=-0.553, 560; P=0.5797; Figure 8), and there was no difference in the mean
length of female and male largemouth bass in Lac des Allemands (F=1.851, 248;
P=0.1746) and Lake Cataouatche (F=0.041, 312; P=0.8384) in each lake or between
females (F=0.221, 310; P=0.6400) or males (F=0.711, 250; P=0.4012) between lakes (Figure
9).
27
10
A
CPUE (#/600 s)
8
A
A
A
6
4
2
B
B
B
B
0
Spring
Summer
Fall
Winter
Season
Figure 6. Mean (± SE) catch per unit effort for largemouth bass collected in Lake
Cataouatche (black bars) and Lac des Allemands (white bars) during the spring (11 April
to 3 May 2012), summer (9 July – 18 July 2012), fall (9 October – 18 October 2012),
and winter (23 January – 24 January 2013) sampling periods. Means with a similar letter
are not different.
28
Table 6. List of all floating and rooted vegetation species encountered.
Floating
Rooted
Water Hyacinth
Eichhornia crassipes
Alligator weed Alternanthera philoxeroides
Common salvinia
Salvinia minima
Bulltongue
Sagittaria lancifolia
Giant duckweed
Spirodela polyrrhiza
Burrmarigold
Bidens laevis
Common Duckweed Lemna minor
Cat-tail
Typhus latifolia
Water lettuce
Pistia stratiotes
Red ludwigia
Ludwigia repens
small duckweed
Lemna valdiviana
Smartweed
Polygonum hydropiperoides
American Lotus
Nelumbo lutea
Wild taro
Colocasia esculenta
White Water Lily
Nymphaea odorata
Watermilfoil
Myriophyllum spicatum
Marsh pennywort
Hydrocotyle umbellata Cabomba
Cabomba caroliniana
Coontail
Ceratophyllum demersum
Eel grass
Vallisneria americana
29
10
A
B
CPUE
8
6
A
4
C
2
D
0
Pre-Isaac
Post-Isaac
B
10
B
CPUE
8
6
A
4
C
2
D
0
Pre-Isaac
Post-Isaac
Figure 7. Mean (± SE) CPUE (#/600 s) of largemouth bass in the presence (white bars)
and absence (black bars) of floating vegetation (A) and rooted vegetation (B). Means
with a similar letter are not different.
30
4000
Weight (g)
3000
2000
1000
0
0
100
200
300
400
500
600
Total Length (mm)
Figure 8. Length-weight relationship for largemouth bass collected from Lac des
Allemands (black squares; R2 = 0.8413) and Lake Cataouatche (white diamonds; R2 =
0.8368) from 9 February 2012 to 24 January 2013. N=587
31
350
173
139
141
111
Total Length (mm)
280
210
140
70
0
Female
Male
Sex
Figure 9. Mean (± SE) total length for female and male largemouth bass collected in
Lake Cataouatche (black bars) and Lac des Allemands (white bars) from 9 February 2012
to 24 January 2013. The number on top of each bar represents sample size.
32
The age of largemouth bass ranged from < 1 to 9 years old, but there were no 8
year old fish collected (Figure 10). The population was dominated by 1 and 2 year old
fish (Figure 11). The only 9 year old was a 459 mm male collected from Lac des
Allemands on 16 July 2012. The oldest female collected was 6 years old, 463 mm long
(TL) and collected from Lake Cataouatche on 10 April 2012. There was no difference in
the mean size (TL) at each age for female and male largemouth bass from either lake
(Figure 10). A von Bertalanffy growth curve was developed for all largemouth bass
collected during this study (Figure 12), where maximum theoretical TL (L∞) = 505.6
mm, von Bertalanffy growth coefficient (k) = 0.3749, and time when TL would
theoretically equal zero (to) = -0.2845.
Diet items identified in largemouth bass from Lake Cataouatche and Lac des
Allemands were not similar (Table 7; Wilks Lambda = 0.7157, F=2.542 1,431 P=0.0002).
Stomachs of largemouth bass collected in the spring from Lake Cataouatche contained
more fish than largemouth bass collected from either lake for the seasons compared
(Table 7; Figure 13). Stomachs of largemouth bass collected from Lac des Allemands in
the spring contained more shrimp than largemouth bass collected from Lac des
Allemands and Lake Cataouatche in the summer (Table 7). Crawfish were found in the
stomachs of largemouth bass from Lake Cataouatche but not in largemouth bass from
Lac des Allemands (Table 7).
Largemouth bass GSI was highest in March and February for both lakes,
decreased during April, and remained low through the summer until increasing from
October to January (Figure 14). GSI ranged from 0.01 to 1.0 for males and from 0.19 to
33
600
Total Length (mm)
500
400
300
200
100
0
0
1
2
3
4
5
Age (years)
6
7
8
9
Figure 10. Mean (± SE) TL at each age for female (black) and male (dots) largemouth
bass collected from Lake Cataouatche and female (white) and male (hatched) largemouth
bass collected from Lac des Allemands.
34
Number of Individuals
80
70
60
50
40
30
20
10
0
0
1
2
3
4
5
Age (years)
6
7
8
9
Figure 11. Total number of female (black) and male (dots) largemouth bass collected
from Lake Cataouatche and female (white) and male (hatched) largemouth bass collected
from Lac des Allemands for each age class.
35
600
Observed
Predicted
Total Length (mm)
500
400
Lt = 505.6(1-e-0.3749 (t + 0.2845))
where:
L∞ = 505.6 mm
k = 0.3749
to = -0.2845
300
200
R2=0.7775
100
N = 640
0
0
1
2
3
4
5
6
Age (years)
7
8
9
10
Figure 12. A von Bertalanffy growth curve, maximum theoretical total length (L∞), von
Bertalanffy growth coefficient (k), and time when total length would theoretically equal
zero (to) for largemouth bass collected in the upper Barataria Estuary from 9 February
2012 to 24 January 2013.
36
Table 7. Mean (± SE) number of diet items in largemouth bass during spring and
summer sampling. Means with similar letters are not different. Fall and winter diets
were not included because of low sample size.
Item
Cataouatche
Spring
Summer
des Allemands
Spring
Summer
Detritus
0.07± 0.03A
0.02± 0.02A
0.04± 0.02A
0.03± 0.03A
Insect
0.14± 0.05A
0.33± 0.12A
0.43± 0.12A
0.42± 0.19A
Crab
0.07± 0.03A
0.04± 0.03A
0.01± 0.01A
0.03± 0.03A
Crawfish
0.04± 0.03A
0.02± 0.02A
0
0
Shrimp
0.20± 0.12
AB
0.11± 0.06
B
0.58± 0.14
A
0.29± 0.14AB
Fish
1.24± 0.14A
0.6± 0.08B
0.77± 0.08B
0.58± 0.08B
Unidentifiable
0.07± 0.03A
0.04± 0.03A
0.05± 0.02A
0.03± 0.03A
37
Percent of Diet
100%
90%
Detritus
80%
Insect
70%
Crab
60%
Crawfish
50%
Shrimp
40%
Fish
30%
Unidentifiable
20%
10%
0%
Cat
dA
Cat
dA
Summer
Spring
Figure 13. Percent of largemouth bass diet by diet category for fish collected from Lake
Cataouatche (Cat) and Lac des Allemands (dA) for the spring and summer season. Fall
and winter seasons are not included because of small sample size. Individuals with empty
stomachs were excluded from analysis.
38
A
8
7
Female GSI
6
5
4
3
2
1
0
0.7
B
0.6
Male GSI
0.5
0.4
0.3
0.2
0.1
0
02/01/12
04/01/12
05/31/12
07/30/12
09/28/12
11/27/12
01/26/13
Figure 14. Mean (± SE) gonadosomatic index (GSI) for female (A) and male (B)
largemouth bass collected from Lac des Allemands (dashed line) and Lake Cataouatche
(solid line) from 9 February 2012 to 24 January 2013.
39
9.16 for females. Male largemouth bass from Lac des Allemands had a higher GSI than
males from Lake Cataouatche in March, May, June and July (Figure 15). Females from
Lac des Allemands had a higher GSI than females from Lake Cataouatche in March, June
and July (Figure 15). GSI values in both lakes indicated that peak spawning occurred
from the end of March to the beginning of April, when water temperatures were
approximately 22˚C.
The observed gonad developmental stages of female largemouth bass (Figure 16)
coincided with seasonal GSI patterns. Females classified as “Spawning Capable” (N =
91; Figure 17) were collected in December, January, February, March, and April and
were the dominant reproductive phase for these five months. “Regressing” individuals
(Figure 18) were collected in April (N = 7) and May (N = 1). “Regenerating” individuals
(Figure 19; N = 87) were collected every month from April through October. Females (N
= 24) entered the “Developing” stage (Figure 20) in October and, females were collected
in this stage until January. Immature individuals (N = 26; Figure 21) were collected in
April, May, June, July and August.
40
8
A
*
7
Female GSI
6
5
4
3
2
* *
1
0
0.7
0.6
B
*
Male GSI
0.5
0.4
0.3
0.2
0.1
*
*
*
0
Feb Mar Apr May Jun
Jul
Aug Sep
Oct
Nov Dec
Jan
Month
Figure 15. Mean (± SE) monthly gonadosomatic index (GSI) for female (A) and male
(B) largemouth bass collected from Lac des Allemands (open bars) and Lake Cataouatche
(dark bars) from 9 February 2012 to 24 January 2013. Asterisks denote a difference in
GSI values between the two lakes.
41
A
100%
15
15
25
9
8
8
9
0
0
2
1
3
12
10
10
6
8
10
10
9
7
5
Percent
75%
50%
25%
0%
B
100%
15
15
Percent
75%
50%
25%
0%
Month
Figure 16. Percent of “developing” (open bar), “spawning capable” (black bar),
“regressing” (light grey bar), and “regenerating” (dark grey bar) gonad developmental
stages of female largemouth bass collected 9 February 2012 to 24 January 2013, from
Lac des Allemands (A) and Lake Cataouatche (B). Numbers above columns indicate the
total number of fish examined each month.
42
Figure 17. Histological section from the ovary of a spawning capable female largemouth
bass (TL = 560 mm) collected on 16 February 2012, in Lake Cataouatche. PGO=primary
growth oocyte; CAO=cortical alveolar oocyte; VTGO=vitellogenic oocyte.
43
Figure 18. Histological section from the ovary of a regressing female largemouth bass
(TL = 345 mm) collected on 18 April 2012, in Lac des Allemands. PGO=primary growth
oocyte.
44
Figure 19. Histological section from the ovary of a regenerating female largemouth bass
(TL = 476 mm) collected on 14 August 2012, in Lac des Allemands. PGO=primary
growth oocyte; CAO=cortical alveolar oocyte.
45
Figure 20. Histological section from the ovary of a developing female largemouth bass
(TL = 264 mm) collected on 20 November 2012, in Lake Cataouatche. PGO=primary
growth oocyte; CAO=cortical alveolar oocyte; VTGO=vitellogenic oocyte.
46
Figure 21. Histological section from the ovary of an immature female largemouth bass
(TL = 176 mm) collected on 27 April 2012, in Lac des Allemands. PGO=primary growth
oocyte; CAO=cortical alveolar oocyte.
47
DISCUSSION
Largemouth bass are native to North America but have been introduced
worldwide because of their popularity as a sport fish (Waters and Noble 2004).
Largemouth bass are a versatile species that can tolerate a wide range of environmental
conditions, but tend to prefer shallow vegetated habitats, which makes electrofishing an
efficient method to collect largemouth bass (Burns and Lantz 1978; Hall 1986; Twedt et
al. 1992; Schoenebeck and Hansen 2005; Siepker et al. 2010). During seasonal sampling,
largemouth bass were the fourth most abundant species collected (N=302), surpassed
only by striped mullet Mugil cephalus (N=634), bluegill Lepomis machrochirus (N=419)
and spotted gar Lepisosteous oculatus (N=371). Smith (2008) also collected a large
amount (60%) of spotted gar in the upper Barataria Estuary. Striped mullet are an
abundant prey species in coastal and estuarine waters (Render et al. 1995), and bluegill
occupy a large portion of the intermediate trophic levels in freshwater systems throughout
North America (Aday et al. 2005). As expected, largemouth bass are a relatively
abundant species along the shorelines of Lake Cataouatche and Lac des Allemands.
Hurricane Isaac made landfall on 28 August 2012 in Venice, Louisiana and turned
westward travelling directly over the Barataria Estuary. Hurricanes can cause localized
fish kills by: 1. creating a surge of runoff pollution, 2. pushing storm-surge associated
saltwater into freshwater areas, and 3. depositing large amounts of organic matter (i.e.,
leaves and branches) that stimulate microbial decomposition and oxygen depletion in the
water column (van Vrancken and O'Connell 2010). In the days following Hurricane
Isaac, the microbial decomposition of organic matter caused hypoxic conditions in much
48
of the upper Barataria Estuary. I observed large fish kills in Lac des Allemands shortly
after the hurricane when dissolved oxygen levels decreased to hypoxic levels, and there
were anecdotal reports (Tim Ruth, personal communication, 15 September 2012) of fish
kills in areas surrounding Lake Cataouatche. Fish kills also occurred in the Atchafalaya
River Basin following the passage of Hurricanes Andrew, Katrina and Rita (Dyer and
McGoodwin 1999; Perret et al. 2010). Dissolved oxygen in Lac des Allemands returned
to normoxic levels (> 2 mg/L) within one month. Although salinity increased in Lake
Cataouatche immediately after Hurricane Isaac, it never reached a level (>1ppt) that
should affect the freshwater fish community. The major effect that Hurricane Isaac had
on the largemouth bass community was the hypoxic conditions and associated fish kill.
Decreases in fish abundance following major hurricanes are common (Hoopes
1975; Stevens et al. 2006). For example, van Vrancken and O’Connell (2010) saw
decreases in several fish species in Bayou Lacombe, Louisiana, following the passage of
Hurricane Katrina in 2005. The relative abundance of largemouth bass for this study was
higher in the spring and summer than in the fall and winter seasons. Because largemouth
bass usually move to deeper water as shallow water temperatures begin to cool in the fall
(Karchesky and Bennett 2004), it is possible that largemouth bass in Lac des Allemands
and Lake Cataouatche moved into deeper canals and bayous before our fall and winter
sampling. However, water temperatures for the fall sampling period were similar to the
spring sampling period, so it is more likely that the hurricane was the direct cause of the
decrease in the relative abundance of largemouth bass.
Largemouth bass prefer to spawn near sand, gravel, or soft mud near vegetation
(reviewed in Brown et al. 2009), making shallow lakes suitable for nesting. Largemouth
49
bass were relatively abundant in Lake Cataouatche and Lac des Allemands, and because
both lakes contain suitable spawning habitat and actively spawning largemouth bass were
collected in both lakes, it is likely that each lake has the necessary habitat for successful
largemouth bass spawning. Successful spawning of largemouth bass has also been
observed in impoundments, reservoirs and rivers in other parts of the country (Boxrucker
1987; Myers and Allen 2005; Wallace and Hartman 2006). The gonad development of
largemouth bass in the upper Barataria Estuary progressed as expected after Hurricane
Isaac, so it is unlikely that the hurricane disrupted the spawning capability of those
largemouth bass that survived the hurricane.
Size at age often differs between males and females of many fish species (Werner
and Gilliam 1984) and the difference in size may be related to timing of maturation. For
some fish species, young males and females are the same size, but mature females tend to
be larger than similar aged mature males (Parker 1992). One reason for the difference in
size between females of different ages is that larger females produce more eggs, and thus
have a higher fitness compared to smaller females (Shine 1988). Davis (2006) found that
female bowfin in the Barataria Estuary were longer than similar aged males and Smith
(2008) found that female spotted gar were longer than similar aged male spotted gar. I did
not detect a difference in total length between male and female largemouth bass, but 93%
of fish collected were 3 years old and younger. The oldest individual collected was 9
years old. A difference in size at age between the sexes may have been detected if more
older fish had been collected.
Many fish populations are characterized by dominant year classes, but there are
generally more young fish than old fish in a given population due to annual mortality
50
(Mathews 1971). One reason the largemouth bass populations from this study were
dominated by one and two year old fish may be that older fish may be less susceptible to
electrofishing (Dolan and Miranda 2003). However, it is more likely that fish older than 3
years are not abundant in the upper Barataria Estuary. Based on the von Bertalanffy
growth curve, it takes largemouth bass in the upper Barataria Estuary 2.96 years to reach
356 mm (14 in), whereas, it takes largemouth bass in the Atchafalaya River Basin 3.4
years to reach the same length (Louisiana Department of Wildlife and Fisheries report.
2012). Because there was no difference in largemouth bass growth rates between Lake
Cataouatche and Lac des Allemands, the factors that affect growth must be similar
between the lakes.
Largemouth bass preferred habitat usually contains aquatic vegetation (Durocher
et al. 1984). In Texas, largemouth bass abundance and recruitment to harvestable size
were positively correlated to submerged aquatic vegetation (SAV; up to 20% coverage;
Durocher et al. 1984). I found that largemouth bass were more abundant in the presence
of rooted and floating vegetation before and after Hurricane Isaac in Lac des Allemands
and Lake Cataouatche. Nutrients released from the Davis Pond Diversion into Lake
Cataouatche stimulated the growth of SAV in and around Lake Cataouatche during the
mid to late 2000s and provided extensive quality largemouth bass habitat (Poirrier et al.
2010). Largemouth bass abundance and size increased in Lake Cataouatche in the years
following the growth of SAV in the lake (Garcia 2013). The Bass Anglers Sportsman
Society (BASS) held the 41st Annual Bass Master’s Classic at Bayou Segnette State Park
in February 2011, and a new three-day total weight record (of 15 fish) of 31.6 kg (69 lbs
11 oz) was established for the tournament. All 15 of these fish were caught on the
51
western end of Lake Cataouatche known locally as the “Tank Ponds,” where the Davis
Pond diversion conveys water into Lake Cataouatche. In a similar system, aquatic
vegetation and largemouth bass abundance increased in Delacroix, Louisiana after the
opening of the Caernarvon Freshwater Diversion (Roberts 1997). Based on aerial
photographs (Google Earth), the amount of aquatic vegetation in Lake Cataouatche has
decreased from approximately 85% coverage in 2007 to almost 0% coverage in 2012.
Also, the amount of SAV measured in Lake Cataouatche during my study was lower than
reported by Poierrier (2010). The relative amount of aquatic vegetation after Hurricane
Isaac during the fall and winter seasons was less than the amount in the spring and
summer seasons. Although the lower temperatures of fall and winter may reduce the
amount of vegetation, Hurricane Isaac was most likely the cause of the reduction in
vegetation in Lake Cataouatche in 2012. The overall reduction in SAV in Lake
Cataouatche has most likely decreased the amount of preferred largemouth bass habitat in
the lake.
The cause of the disappearance of SAV in Lake Cataouatche since the late 2000’s
is unclear, but the physical components of the substrate may be partly responsible. Teal et
al. (2012) note that diversions can increase the rate of organic material decomposition
leading to a decrease in soil strength of affected areas. The annual mean discharge of the
Davis Pond diversion was highest in the 4 years leading up to this study. This is also
when the SAV in Lake Cataouatche began to decrease. Since the Davis Pond Diversion
opened in 2002, the bottom of Lake Cataouatche has been covered by loose organic
material (DeLaune et al. 2008). This loose material may provide a substrate that is soft
and prevents shallow SAV roots from being able to withstand pressure from wind or
52
wave activity, allowing them to easily be uprooted. Perhaps the addition of organic
material into Lake Cataouatche from 2008 to 2011 was enough to almost completely
prevent any successful germination or growth of SAV in Lake Cataouatche. Ultimately,
the Davis Pond Diversion was responsible for the growth of SAV in Lake Cataouatche
and may also be responsible for its disappearance.
Studies have shown that removal of SAV from lakes has not negatively affected
largemouth bass distribution, abundance, and growth (Colle et al. 1989; Pothoven et al.
1999), but the absence of aquatic vegetation in Lake Cataouatche has likely directly or
indirectly affected the abundance of largemouth bass. Mr. George E. Garcia, owner of
Pier 90 marina (one of two marinas anglers use to gain access to Lake Cataouatche), has
observed a decrease in the number of anglers using his marina since the disappearance of
SAV from Lake Cataouatche. Mr. Garcia often weighs largemouth bass caught from
Lake Cataouatche by anglers using his marina, and he said that from 2004 to 2011, there
were around 20 largemouth bass over 4.5 kg (10 lbs) and hundreds more between 2.3 and
4.5 kg (5 and 10 lbs) caught in Lake Cataouatche. During the same time, local bass clubs
reported few catches of trophy largemouth bass in Lac des Allemands. Since 2011 and
the disappearance of SAV in the lake, Mr. Garcia says that business has decreased and
the largest bass he has seen was 2 kg (4.5 lbs; George E. Garcia, personal
communication. 20 March 2013). Also, only one largemouth bass of trophy size (566
mm; 3,662 g) was collected by electrofishing during this study. Because largemouth bass
prefer to inhabit vegetated areas, the reduction in preferred habitat may have concentrated
the largemouth bass and made them more vulnerable to angling pressure than if they were
dispersed throughout the lake. Many other states practice voluntary or mandatory
53
(minimum length or slot limits) catch and release of largemouth bass (Quinn 1989;
Philipp et al. 1997; Myers et al. 2008; Carlson and Isermann 2010), but there is no
minimum length or slot limit for largemouth bass in the upper Barataria Estuary. The
removal of large numbers of largemouth bass from Lake Cataouatche by local anglers
may be partly responsible for the decrease in size and numbers of largemouth bass in the
lake as reported by Mr. Garcia, other local anglers, and as I have personally witnessed
since the disappearance of SAV in Lake Cataouatche.
Largemouth bass have a generalist feeding strategy and their diet depends on the
habitat and available prey (Middaugh 2011). Largemouth bass collected in the spring
from Lake Cataouatche had more fish in their stomachs than largemouth bass collected
from Lac des Allemands in the spring or from either lake in the summer. Prey fish were
likely more accessible to largemouth bass in Lake Cataouatche during the spring than
during any other time. Fish kills associated with Hurricane Isaac may have reduced prey
fish populations in both lakes, but post hurricane largemouth bass sample size was too
small to make inferences about diets for the fall and winter samples. Bettoli et al. (1992)
found that small largemouth bass (60 mm) began to consume fish following the removal
of all SAV in Lake Conroe, Texas, and this resulted in faster first year growth of all
largemouth bass hatched after the SAV removal. Young largemouth bass in Lake
Cataouatche may also be shifting earlier towards a piscivorous diet since the
disappearance of SAV in the lake. Crawfish were found in the stomachs of bass from
Lake Cataouatche but not in bass from Lac des Allemands. Crawfish are common in the
Mississippi River floodplains (Dellenbarger and Luzar 1988), but the lack of a floodpulse
may decrease crawfish abundance or availability in the upper Barataria Estuary (Manley
54
2012). If the Davis Pond Diversion is operated to provide the upper Barataria Estuary
with a spring floodpulse, the crawfish population may increase.
Overall, 17% of largemouth bass stomachs examined from Lake Cataouatche and
Lac des Allemands were empty. This is similar to the 18% empty largemouth bass
stomachs that Christensen and Moore (2007) found in a lake in Washington but less than
the 50% empty stomachs that Lewis et al. (1974) found in largemouth bass in Illinois.
Largemouth bass diets are likely a result of the availability of suitable forage, and empty
stomachs may serve as a cue to feed (Lewis et al. 1974). The relatively low percent of
empty stomachs in largemouth bass in the upper Barataria Estuary suggests that there is
either an adequate supply of suitable forage or catch efficiency of prey is high for
largemouth bass in both lakes.
Many fishes of floodplain systems evolved to time major life events, such as
spawning and feeding tactics, to coincide with the river’s annual floodpulse (Junk et al.
1989; Bayley 1995). Most of South Louisiana, including the Barataria Estuary, was once
inundated by the Mississippi River’s annual spring floodpulse. Since the construction of
levees along the Mississippi River, the Barataria Estuary no longer receives an annual
floodpulse, and water levels depend on unpredictable rainfall (Inoue et al. 2008). Bowfin
spawning in the upper Barataria Estuary is negatively affected by the lack of a floodpulse
because low water during spawning season limits spawning habitat (Davis 2006). It
appears largemouth bass spawning is not negatively affected because they are able to use
the lake edge and do not rely on an inundated floodplain. Lake Cataouatche and Lac des
Allemands may serve as source populations of largemouth bass for other portions of the
upper Barataria Estuary. Raibley et al. (1997) found that largemouth bass spawning was
55
negatively affected during low water years when floodplains did not remain inundated, so
largemouth bass spawning in the upper Barataria Estuary may be limited in upstream and
backwater areas that may not provide adequate spawning habitat. Freshwater diversions
may provide a systematic floodpulse in affected areas, but strategized operation of these
diversions is critical. Local fish that are floodplain dependent may benefit from
diversions if the diversions are operated to provide a floodpulse such as the Mississippi
River would naturally provide, which may subsequently increase prey abundance for
largemouth bass.
Temperature and photoperiod directly affect fish reproduction (Junk 1999).
Southern latitudes warm earlier and quicker than northern latitudes, so southern fish
populations likely spawn earlier in the year than northern populations. In Canada,
largemouth bass spawn in the summer, sometimes as late as August, when water
temperatures reach 16.7 ˚C (Scott and Crossman 1973). Largemouth bass in Puerto Rico
begin spawning in mid-January in 25˚C waters (Waters and Noble 2004). Largemouth
bass spawning in Lake Cataouatche and Lac des Allemands occurred from late March to
early April. Spawning in the upper Barataria Estuary began when waters reached
approximately 22˚C, similar to the largemouth bass spawning temperatures in Lake
Mead, Oklahoma reported by Allen and Romero (1975). Although largemouth bass in
Lake Cataouatche and Lac des Allemands spawned at approximately the same time,
females and males from Lac des Allemands had higher GSI values than fish from Lake
Cataouatche for some months. Differences in GSI values in different populations of the
same species usually indicate a difference in spawning season. Because our two
populations of largemouth bass spawned at the same time, perhaps fish from Lac des
56
Allemands invest more energy into reproduction than fish from Lake Cataouatche. It is
also possible that largemouth bass in Lac des Allemands are producing more or larger
eggs than largemouth bass in Lake Cataouatche, but neither fecundity nor egg diameter
was measured for this study.
Gonad histology is used to accurately define spawning seasons of fishes and
classify reproductive stages within the reproductive cycle (Jackson et al. 2006). Very
little has been published in the peer-reviewed literature on the gonad histology of
largemouth bass (James 1946), and this study may be the first to use the Brown-Peterson
et al. (2011) reproductive classification system for largemouth bass. The use of this
classification system in this study suggests that this classification system may be useful
for managers that need to make decisions about largemouth bass regulations. By
combining largemouth GSI data and gonad histology, I was able to determine when
largemouth bass in the upper Barataria Estuary are spawning, and by combining age data
with gonad histology, I determined that female largemouth bass in the upper Barataria
Estuary do not mature in their first year. Tidwell (2000) reports that largemouth bass may
mature in their first year if they reach 255 mm, but the largemouth bass I collected did
not reach that length during the first year. It seems that most female largemouth bass in
the upper Barataria Estuary first spawn at approximately two years of age.
Freshwater diversions increase rates of vertical accretion in surrounding marshes
(DeLaune et al. 2003; Snedden et al. 2007) and decrease salinity in affected areas
(Sanders and Piasecki 2002), and marshes affected by freshwater diversions process and
remove dissolved nitrogen from river water (DeLaune et al. 2005). If operated such as
the Mississippi River would naturally operate, freshwater diversions may also be useful
57
for preventing hypoxia in swamps and marshes following the passage of hurricanes.
There is a proposed freshwater diversion that would reintroduce Mississippi River water
into Lac des Allemands through Bayou Chevreuil (Mississippi River 2004). This
diversion may stimulate vegetative and fisheries growth in Lac des Allemands while
helping to maintain normoxic dissolved oxygen levels in the upper Barataria Estuary.
Ultimately, Lac des Allemands and Lake Cataouatche both contain the necessary
habitat to support largemouth bass populations, but natural disasters such as hurricanes
may be a limiting factor controlling abundance and size of largemouth bass in the upper
Barataria Estuary. Although, largemouth bass abundance and size increased in response
to the opening of a freshwater diversion in Caernarvon, Louisiana (Roberts 1997), I
cannot make the same conclusion based on the results of my study. Perhaps there would
have been a difference between the largemouth bass populations in Lac des Allemands
and Lake Cataouatche if this study was conducted in the mid to late 2000s while SAV
was abundant in Lake Cataouatche. If SAV does not return in Lake Cataouatche, we may
continue to see a decrease in the local largemouth bass population. Perhaps the proposed
freshwater diversion through Lac des Allemands would stimulate vegetative and fisheries
production in much of the upper Barataria Estuary.
58
FUTURE RECOMMENDATIONS
More research is needed on freshwater diversions and their effects on estuarine
habitats and fish populations. Freshwater diversions are still relatively new, and their long
term effects are not well understood. However, to benefit the entire Barataria Estuary, I
believe the Davis Pond diversion should be operated to provide a floodpulse, such as the
Mississippi River would naturally provide. South Louisiana was built by the Mississippi
River floodpulse, and organisms living here have evolved to utilize this pulse. A
freshwater diversion through Lac des Allemands may be beneficial to the Barataria
Estuary. Such a diversion could provide the Lac des Allemands area with a constant
supply of fresh, oxygenated water, and may result in smaller fish kills during periods of
low oxygen. There should also be a study that focuses on aquatic vegetation associated
with freshwater diversions, and examines the reasons behind the disappearance of the
aquatic vegetation in Lake Cataouatche. Reestablishing aquatic vegetation in Lake
Cataouatche may allow the largemouth bass production to rebound to the levels seen in
the mid to late 2000s.
To provide the best understanding and be able to provide management strategies
for largemouth bass in the upper Barataria Estuary, a long term study of the largemouth
bass and their habitat use is needed in the upper Barataria Estuary. Unlike largemouth
bass found in impoundments and reservoirs throughout the country, largemouth bass in
the Barataria Estuary are not confined by structural boundaries, and are able to move
freely throughout the estuary. A telemetry study investigating the movements of
largemouth bass in the upper Barataria Estuary would provide insight as to where
largemouth bass take refuge during periods of low oxygen. Efforts could then replicate
59
refuges, possibly leading to an increase in the number of largemouth bass that survive
hurricanes and other low oxygen events. Also, a fecundity study of the largemouth bass
in the upper Barataria Estuary may help explain the differences in GSIs between the two
populations observed in this study.
60
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74
APPENDIX I
Appendix I. Sampling date (Month/ Day/ Year), Lake (DA; des Allemands, Cat;
Cataouatche), Plot, Fish ID, Length (mm), Weight (g), and Sex of largemouth bass
collected from Lac des Allemands and Lake Cataouatche from 2 February 2012 to 24
January 2013.
Date
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
Lake Plot
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
DA
.
Cat
.
Cat
.
Cat
.
Cat
.
Cat
.
Cat
.
ID
4012
4010
4004
4021
4028
4022
4009
4033
4016
4023
4014
4002
4007
4019
4003
4008
4001
4020
4025
4013
4018
4030
4006
4038
4041
4042
4040
4037
4039
4051
4062
4066
4065
4056
4060
Length (mm)
252
289
299
310
322
329
319
338
340
333
366
373
445
292
267
300
311
336
346
395
451
465
525
457
311
324
356
411
453
335
360
403
391
405
424
75
Weight (g)
266
320
388
446
470
498
518
540
588
598
680
948
1270
308
258
356
472
514
644
868
1512
1802
2658
1650
430
458
618
1176
1448
458
644
886
966
1076
1160
Sex
m
m
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
m
f
f
f
f
f
m
m
m
m
m
m
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
4067
4071
4050
4048
4052
4044
4053
4061
4047
4069
4049
4058
4046
4045
4064
4070
4063
4055
4068
1961
1975
4077
1965
1974
1966
1967
1955
1969
1954
1971
1960
1973
1972
1970
1952
1968
1963
1957
1953
1956
1964
1959
1958
433
450
451
471
279
299
293
319
328
350
353
381
395
409
405
418
430
467
560
266
322
323
341
346
340
385
380
385
420
256
261
298
319
313
320
325
328
338
345
380
406
471
499
76
1204
1374
1602
1850
270
362
392
468
498
667
734
856
966
1062
1070
1238
1296
1500
3662
280
428
430
532
554
572
708
802
850
1022
216
224
386
390
404
442
548
552
566
576
788
1066
1570
2018
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
3/15/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
64
68
72
46
72
64
44
68
64
64
64
46
44
68
72
64
46
1962
4100
4080
4099
4097
4098
4101
4081
4084
4076
4088
4095
4096
4094
4085
4078
4086
4082
4079
4083
4087
4091
4092
4089
4090
4093
4115
4104
4116
4073
4118
4109
4122
4102
4113
4111
4106
4072
4121
4103
4117
4110
4075
490
270
311
305
342
341
353
349
451
465
475
270
265
275
319
325
355
364
390
397
395
410
421
448
445
491
155
160
175
199
255
270
253
279
278
289
275
285
300
295
174
184
205
77
2036
296
416
428
546
566
566
664
1450
1458
1464
264
274
282
398
500
664
702
896
932
966
986
1174
1384
1612
1972
48
58
70
108
196
254
266
270
280
310
310
316
318
372
70
82
110
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
m
m
m
f
f
f
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
64
64
46
64
64
44
44
34
6
34
20
20
20
14
6
14
34
20
20
20
6
14
14
20
14
6
14
20
34
20
20
54
54
55
55
55
54
54
54
54
54
55
54
4108
4112
4074
4105
4107
4119
4120
4143
4124
4145
4133
4139
4134
4129
4125
4131
4146
4136
4142
4140
4123
4127
4130
4135
4132
4126
4128
4138
4144
4137
4141
4161
4151
4168
4166
4164
4149
4158
4154
4157
4155
4163
4148
220
250
256
302
463
254
269
165
169
180
184
189
196
210
237
243
252
299
310
194
210
255
256
271
300
285
298
300
319
345
364
174
190
229
250
266
270
282
274
272
309
312
365
78
136
202
224
394
1580
198
264
52
60
76
78
82
124
134
178
202
226
372
372
94
118
226
240
250
312
320
354
368
464
616
764
70
92
168
216
222
244
256
268
290
364
392
628
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
m
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/18/2012
4/18/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
57
57
57
55
57
55
55
55
55
55
55
55
57
54
54
55
54
54
54
54
55
54
11
8
26
25
26
25
29
29
26
26
25
29
26
29
25
25
26
25
25
25
25
.
.
.
.
.
.
.
.
.
.
.
4165
4169
4150
4159
4167
4153
4152
4156
4160
4162
4147
4171
4170
4193
4180
4187
4177
4197
4196
4183
4194
4174
4195
4190
.
4178
4181
4188
4176
4175
4182
4179
269
270
286
259
277
274
284
266
291
296
300
164
162
191
200
210
224
305
275
286
305
397
345
267
190
191
203
226
268
274
285
299
314
334
378
313
137
145
165
172
198
205
219
79
196
240
250
286
311
314
320
330
341
370
401
54
54
92
106
130
146
390
274
318
444
948
558
236
89
92
113
164
261.5
279.5
350
365
486
534
792
360
34
37.5
56.5
66
98
119
142
U
U
U
U
U
U
U
U
U
U
U
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
U
f
f
f
f
f
f
f
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
26
26
26
26
25
25
26
26
58
62
62
62
62
62
62
58
62
41
41
39
41
41
41
41
39
39
41
41
39
39
39
61
66
66
66
66
66
66
66
.
.
.
.
4192
4186
4185
4191
4172
4173
4189
4184
4199
4203
4200
.
.
.
4202
4198
4201
4211
4213
4209
.
.
.
.
4208
4205
4210
4212
4204
4206
4207
4214
4217
4216
.
.
.
.
4215
124
104
110
116
274
293
359
299
295
312
326
280
221
315
353
285
280
278
219
298
354
170
166
212
251
264
273
300
176
186
215
242
269
278
248
175
191
214
248
258
278
291
313
221
230
269
271
80
310.5
371
629
351
393
400
528.5
345
154
382
674
250
292
328
134
342
644
54
54
114
204
241
252
380
68
82
128
190
244
276
196
72
92
136
190
218
310
326
366
150
174
262
264
f
f
f
f
f
f
f
f
m
m
m
U
U
U
f
f
f
m
m
m
U
U
U
U
f
f
f
f
f
f
f
f
f
m
U
U
U
U
f
f
f
f
f
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
125
121
122
103
105
112
102
106
100
120
107
101
109
113
117
111
119
123
114
108
115
127
142
137
141
129
126
147
148
143
146
149
150
145
139
136
138
144
128
140
135
134
133
300
241
283
276
288
302
294
292
315
325
292
247
300
290
292
292
319
344
351
362
290
221
293
313
334
322
377
230
239
290
296
295
305
312
304
310
333
261
330
333
345
373
355
81
354
212
288
324
324
362
376
382
422
430
442
212
306
306
342
352
432
584
590
668
356
144
326
432
434
440
778
164
204
320
324
348
348
352
370
402
558
248
544
546
568
692
692
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
5/23/2012
5/23/2012
5/23/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
131
130
132
157
175
172
171
152
156
158
167
162
166
164
168
169
165
163
153
174
173
159
155
170
161
160
151
154
199
193
194
189
192
190
197
200
180
181
196
188
178
182
183
379
404
457
239
264
254
269
274
297
232
280
290
314
320
341
355
340
432
467
250
293
312
320
327
355
365
345
353
252
276
293
314
264
296
283
299
298
298
285
283
283
302
300
82
838
1020
1136
184
236
256
266
292
380
188
284
332
428
456
540
678
684
1132
1574
184
254
410
440
482
568
682
710
710
222
312
390
482
270
340
344
352
356
356
372
384
390
396
408
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
.
.
.
.
.
.
.
.
.
.
61
64
64
62
66
66
64
66
61
65
64
61
66
65
66
63
61
64
61
66
64
66
64
64
66
2
3
6
2
3
6
2
3
176
186
185
184
198
187
179
191
195
177
4228
4238
4237
4218
4223
4224
4236
4219
4232
4240
4239
4229
4226
4241
4221
4242
4227
4233
4230
4222
4234
4225
4231
4235
4220
4265
4259
4250
4262
4261
4248
4264
4256
295
282
295
287
304
312
331
342
352
468
95
252
240
285
290
291
309
316
199
202
209
244
246
287
286
309
324
380
408
216
270
321
319
320
429
282
314
230
252
267
282
293
282
83
320
328
340
350
402
446
542
550
556
1614
12
232
238
298
368
384
432
458
96
106
130
186
216
352
374
414
450
812
1012
140
264
484
490
494
1514
296
434
168
230
248
310
328
356
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
m
m
m
m
m
m
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
2
2
6
4
3
6
6
4
6
6
6
4
2
6
3
18
23
10
42
39
18
18
25
23
35
35
39
23
18
10
51
48
51
59
51
51
51
55
51
59
51
48
59
4260
4258
4245
4252
4254
4251
4249
4253
4244
4246
4247
4255
4263
4243
4257
4274
4278
4267
4268
4270
4271
4273
4275
4277
4280
4279
4269
4276
4272
4266
4286
4296
4285
4290
4289
4284
4288
4293
4283
4291
4282
4295
4292
284
306
315
315
390
100
235
251
297
286
303
340
424
450
440
215
229
228
253
262
293
304
351
242
252
310
326
359
459
445
223
246
240
285
194
267
270
275
295
281
316
345
249
84
356
396
422
452
940
12
172
206
364
392
396
576
1122
1554
1580
136
140
174
236
248
422
460
626
206
238
416
486
574
1338
1220
148
204
208
372
106
260
300
322
328
330
456
714
224
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
f
f
f
f
f
m
m
m
m
m
m
m
m
f
7/18/2012
7/18/2012
7/18/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
51
55
51
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
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.
.
.
.
.
.
.
.
.
.
4281
4294
4287
223
225
219
209
215
202
220
213
212
216
217
206
208
214
205
203
224
211
221
218
222
210
204
207
201
247
249
242
243
238
229
245
248
232
239
228
235
230
241
227
271
272
315
291
295
322
334
378
372
287
292
284
296
302
301
322
325
416
425
251
303
283
299
322
308
322
345
454
245
264
264
291
363
405
242
265
268
288
327
316
332
326
374
85
288
282
408
296
362
432
514
840
874
306
348
350
350
386
396
482
538
1094
1346
222
356
368
394
418
428
456
576
1496
200
248
254
308
700
1042
210
248
286
326
482
500
512
522
730
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
10/9/2012
10/9/2012
10/9/2012
10/9/2012
10/11/2012
10/11/2012
10/16/2012
10/18/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
Cat
DA
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
72
67
72
67
19
26
51
2
231
250
240
246
244
236
233
234
237
226
256
252
253
251
254
271
266
262
265
270
267
255
257
261
258
274
273
275
272
268
264
269
263
259
260
4299
4297
4300
4298
4302
4301
4303
4304
380
244
309
320
314
337
376
385
393
476
396
404
415
469
478
273
290
284
291
303
295
316
345
444
474
247
248
262
275
280
283
297
292
304
303
317
320
320
320
309
316
312
352
86
828
188
424
484
486
650
842
884
1078
1656
772
938
1060
1530
1636
266
272
290
328
366
372
462
464
1322
1648
172
206
236
240
276
300
322
352
368
432
440
464
464
420
396
422
400
570
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
m
m
m
f
m
m
m
m
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
283
276
280
279
277
282
278
291
284
290
288
289
286
281
287
285
295
293
296
294
298
292
297
308
311
307
305
301
320
319
300
312
302
303
314
306
313
317
322
321
316
309
315
322
321
335
331
344
356
480
238
260
290
295
295
303
341
356
446
257
286
304
325
360
279
310
296
313
304
348
255
260
290
290
298
298
315
347
335
329
266
264
270
281
285
290
87
418
438
480
488
510
724
1650
184
220
301
308
322
384
548
595
1278
210
296
368
434
676
250
414
342
388
390
528
198
218
290
296
310
322
434
502
508
510
202
220
238
274
288
310
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
m
m
m
m
m
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
11/20/2012
11/20/2012
11/20/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
66
.
.
.
.
14
33
318
310
304
341
340
337
333
331
332
330
325
324
327
323
339
338
335
334
336
328
329
326
348
342
345
347
346
344
343
4305
349
351
350
352
4307
4306
308
316
378
267
278
283
288
293
305
312
273
282
287
322
259
256
256
296
287
315
352
291
279
300
323
275
267
282
315
349
316
307
390
363
364
310
88
340
410
826
236
280
294
324
336
360
412
246
278
312
470
214
202
212
356
384
486
626
318
268
338
416
228
244
290
384
538
430
416
734
744
734
416
f
f
f
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
m
m
m
f
f
f
f
f
m
f
f
f
f
m
APPENDIX II
Appendix II. Sampling date (Month/ Day/ Year), Lake (DA; des Allemands, Cat;
Cataouatche), Fish ID, Gonad Weight (g), GSI, Sex, Reproductive Stage (SC; Spawning
Capable), and Age of largemouth bass collected from Lac des Allemands and Lake
Cataouatche from 9 February 2012 to 24 January 2013.
Date
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/9/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/15/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
Lake
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
ID
4003
4019
4008
4001
4020
4025
4013
4018
4030
4006
4012
4010
4004
4021
4028
4022
4009
4033
4016
4023
4014
4002
4007
4041
4042
4040
4037
4039
4038
4052
4044
4053
4061
4047
Gonads
(g)
16.13
2.06
10.61
29.61
29.29
38.22
49.42
75.00
73.00
176.00
2.39
3.06
2.05
2.43
2.16
2.87
2.17
2.34
2.04
4.05
2.14
4.83
5.74
24.70
21.13
39.50
68.10
90.10
7.10
4.64
6.77
3.65
29.10
13.39
GSI
6.25
0.67
2.98
6.27
5.70
5.94
5.69
4.96
4.05
6.62
0.90
0.96
0.53
0.54
0.46
0.58
0.42
0.43
0.35
0.68
0.31
0.51
0.45
5.74
4.61
6.39
5.79
6.22
0.43
1.72
1.87
0.93
6.22
2.69
89
Sex
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
m
f
f
f
f
f
Stage
SC
developing
SC
SC
SC
SC
SC
SC
SC
SC
.
.
.
.
.
.
.
.
.
.
.
.
.
SC
SC
SC
SC
SC
.
SC
SC
SC
SC
SC
Age
1.86
1.86
1.86
1.86
1.86
1.86
2.86
3.86
4.86
4.86
1.86
1.86
1.86
1.86
1.86
1.86
1.86
1.86
1.86
1.86
2.86
2.86
4.86
1.87
1.87
1.87
3.87
4.87
3.87
1.88
1.88
1.88
1.88
1.88
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
2/16/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
3/15/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
4069
4049
4058
4046
4045
4064
4070
4063
4055
4068
4051
4062
4066
4065
4056
4060
4067
4071
4050
4048
1971
1960
1973
1972
1970
1952
1968
1963
1957
1953
1956
1964
1959
1958
1962
1961
1975
4077
1965
1974
1966
1967
1955
34.70
44.10
36.30
48.40
68.60
70.80
54.90
83.60
76.50
228.00
1.91
3.39
6.29
2.88
4.84
3.99
4.34
5.30
5.88
16.88
12.50
18.50
32.00
23.50
37.00
19.04
32.00
42.28
31.66
30.40
45.00
75.50
72.00
104.00
121.50
1.77
3.50
2.61
2.07
2.13
2.54
3.67
4.41
5.20
6.01
4.24
5.01
6.46
6.62
4.43
6.45
5.10
6.23
0.42
0.53
0.71
0.30
0.45
0.34
0.36
0.39
0.37
0.91
5.79
8.26
8.29
6.03
9.16
4.31
5.84
7.66
5.59
5.28
5.71
7.08
4.59
5.15
5.97
0.63
0.82
0.61
0.39
0.38
0.44
0.52
0.55
90
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
.
.
.
.
.
.
.
.
.
.
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
.
.
.
.
.
.
.
.
2.88
1.88
2.88
2.88
4.88
2.88
3.88
3.88
4.88
5.88
2.88
2.88
4.88
1.88
2.88
5.88
4.88
4.88
5.88
7.88
1.95
1.95
1.95
1.95
1.95
1.95
1.95
1.95
1.95
1.95
2.95
1.95
4.95
4.95
5.95
1.95
1.95
1.95
2.95
1.95
1.95
1.95
2.95
3/15/2012
3/15/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
3/16/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
1969
1954
4095
4096
4094
4085
4078
4086
4082
4079
4083
4087
4091
4092
4089
4090
4093
4100
4080
4099
4097
4098
4101
4081
4084
4076
4088
4117
4110
4075
4108
4119
4112
4074
4120
4105
4107
4115
4104
4116
4073
4118
4109
2.50
7.38
11.00
17.50
22.50
23.50
20.00
19.00
50.00
28.00
33.00
22.00
53.50
51.00
45.50
41.00
60.00
1.35
0.62
0.95
2.08
0.90
1.48
2.40
5.45
4.83
7.14
0.25
0.40
0.36
0.67
3.70
1.33
1.34
6.01
2.63
25.00
0.04
0.05
0.05
0.11
0.50
0.34
0.29
0.72
4.17
6.39
7.98
5.90
4.00
2.86
7.12
3.13
3.54
2.28
5.43
4.34
3.29
2.54
3.04
0.46
0.15
0.22
0.38
0.16
0.26
0.36
0.38
0.33
0.49
0.36
0.49
0.33
0.49
1.87
0.66
0.60
2.28
0.67
1.58
0.07
0.09
0.07
0.10
0.26
0.13
91
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
.
.
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
.
.
.
.
.
.
.
.
.
.
immature
immature
immature
immature
SC
immature
regenerating
SC
regenerating
regressing
.
.
.
.
.
.
2.95
5.95
1.96
1.96
1.96
1.96
2.96
2.96
2.96
4.96
2.96
3.96
2.96
3.96
5.96
4.96
4.96
1.96
1.96
1.96
2.96
1.96
2.96
2.96
3.96
4.96
4.96
1.02
1.02
1.02
1.02
2.02
2.02
2.02
2.02
2.02
6.02
1.02
1.02
1.02
1.02
2.02
2.02
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/10/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/11/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
4122
4102
4113
4111
4106
4072
4121
4103
4140
4123
4127
4130
4135
4132
4126
4128
4138
4144
4137
4141
4143
4124
4145
4133
4139
4134
4129
4125
4131
4146
4136
4142
4165
4169
4150
4159
4167
4153
4156
4160
4152
4162
4147
1.28
0.88
0.64
0.78
0.51
1.04
1.30
1.28
0.28
2.37
12.50
15.32
10.69
9.68
6.68
11.09
9.75
13.64
29.20
35.70
0.03
0.04
0.03
0.04
0.82
0.08
0.29
0.48
0.55
0.40
0.10
1.10
0.167
0.207
0.381
0.366
0.491
0.567
2.795
12.913
4.242
10.103
25.783
0.48
0.32
0.23
0.25
0.16
0.33
0.41
0.34
0.30
2.01
5.53
6.38
4.28
3.10
2.09
3.13
2.65
2.94
4.74
4.67
0.06
0.07
0.04
0.05
1.00
0.07
0.22
0.27
0.27
0.18
0.03
0.30
0.31
0.38
0.41
0.35
0.38
0.39
1.02
4.06
1.09
2.28
2.72
92
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
.
.
.
.
.
.
.
.
immature
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
SC
.
.
.
.
.
.
.
.
.
.
.
.
immature
immature
immature
immature
immature
immature
SC
SC
regressing
SC
SC
2.02
2.02
2.02
2.02
2.02
2.02
2.02
3.02
1.03
1.03
2.03
2.03
2.03
2.03
2.03
2.03
2.03
2.03
2.03
3.03
1.03
1.03
1.03
1.03
1.03
1.03
1.03
2.03
2.03
2.03
2.03
2.03
1.03
1.03
1.03
1.03
1.03
1.03
2.03
2.03
2.03
2.03
5.03
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/13/2012
4/18/2012
4/18/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
4161
4151
4168
4166
4164
4149
4158
4154
4157
4155
4163
4148
.
.
.
.
.
.
.
.
.
.
.
4170
4171
4178
4181
4188
4176
4175
4182
4179
4192
4184
4191
4186
4172
4173
4189
4185
4193
4180
4187
0.068
0.021
0.436
0.362
0.469
0.511
0.481
0.822
1.036
.
0.776
0.61
.
.
.
.
.
.
.
.
.
.
.
3.41
4.339
0.143
0.107
0.23
0.277
0.326
0.405
0.447
2.403
5.577
5.169
2.032
3.578
2.709
4.475
4.527
0.128
0.032
0.042
0.10
0.02
0.26
0.17
0.21
0.21
0.19
0.31
0.36
.
0.20
0.10
.
.
.
.
.
.
.
.
.
.
.
1.44
0.78
0.42
0.29
0.41
0.42
0.33
0.34
0.31
0.77
1.62
1.47
0.55
0.91
0.68
0.85
0.72
0.14
0.03
0.04
93
m
m
m
m
m
m
m
m
m
m
m
m
U
U
U
U
U
U
U
U
U
U
U
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
SC
regressing
immature
immature
immature
immature
immature
immature
immature
regenerating
SC
regressing
regenerating
regressing
regressing
regressing
regenerating
.
.
.
1.03
1.03
1.03
2.03
2.03
2.03
2.03
2.03
2.03
2.03
2.03
3.03
.
.
.
.
.
.
.
.
.
.
.
2.05
2.05
1.05
1.05
1.05
1.05
1.05
1.05
1.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
2.05
1.05
1.05
1.05
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/19/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/26/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
4/27/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/3/2012
5/16/2012
5/16/2012
5/16/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
4177
4197
4196
4183
4194
4174
4195
4190
.
4202
4198
4201
4199
4203
4200
.
.
.
4208
4205
4210
4212
4207
4204
4206
4211
4213
4209
.
.
.
.
4214
4217
4215
4216
.
.
.
.
124
104
101
0.094
0.362
0.978
0.83
0.971
1.512
1.633
1.902
.
0.52
2.52
4.19
0.38
0.36
0.64
.
.
.
0.281
0.257
0.449
1.159
3.096
1.722
1.573
.
.
0.138
.
.
.
.
0.229
0.321
2.093
0.057
.
.
.
.
0.3973
0.5884
0.396
0.06
0.14
0.35
0.24
0.27
0.31
0.31
0.24
.
0.39
0.74
0.65
0.25
0.10
0.10
.
.
.
0.41
0.31
0.35
0.61
1.58
0.71
0.57
.
.
0.12
.
.
.
.
0.32
0.35
0.57
0.04
.
.
.
.
0.26
0.34
0.19
94
m
m
m
m
m
m
m
m
U
f
f
f
m
m
m
U
U
U
f
f
f
f
f
f
f
m
m
m
U
U
U
U
f
f
f
m
U
U
U
U
f
f
f
.
.
.
.
.
.
.
.
.
immature
regenerating
regressing
.
.
.
.
.
.
immature
immature
immature
regenerating
regressing
regenerating
regenerating
.
.
.
.
.
.
.
.
.
regenerating
.
.
.
.
.
.
.
immature
1.05
2.05
2.05
2.05
2.05
3.05
2.05
3.05
.
1.07
2.07
3.07
1.07
2.07
3.07
.
.
.
1.07
1.07
1.07
2.07
2.07
2.07
2.07
1.07
1.07
1.07
.
.
.
.
1.09
1.09
2.09
1.09
.
.
.
.
1.12
1.12
1.12
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/16/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
5/23/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
110
116
109
113
117
111
125
115
119
123
114
108
121
122
103
105
112
102
106
100
120
107
127
144
142
137
141
129
128
140
135
134
133
126
131
130
132
147
148
143
146
149
150
1.696
1.2815
1.7685
1.2335
1.6854
2.886
2.0843
2.3123
2.1175
2.9244
3.2279
5.8852
0.043
0.1581
0.1599
0.0307
0.1452
0.194
0.055
0.096
0.1559
0.0703
0.5323
1.39
2.567
2.387
2.118
2.4045
2.541
3.035
3.854
3.947
4.029
3.9113
4.546
4.528
6.267
0.0733
0.0337
0.409
0.517
0.397
0.468
0.65
0.49
0.58
0.40
0.49
0.82
0.59
0.65
0.49
0.50
0.55
0.88
0.02
0.05
0.05
0.01
0.04
0.05
0.01
0.02
0.04
0.02
0.37
0.56
0.79
0.55
0.49
0.55
0.47
0.56
0.68
0.57
0.58
0.50
0.54
0.44
0.55
0.04
0.02
0.13
0.16
0.11
0.13
95
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
.
.
regenerating
regenerating
regenerating
regenerating
.
regressing
regenerating
regenerating
regenerating
regenerating
.
.
.
.
.
.
.
.
.
.
.
regenerating
.
.
.
.
regenerating
regenerating
regenerating
regenerating
regenerating
.
regenerating
regenerating
regenerating
.
.
.
.
.
.
2.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
3.12
1.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
2.12
3.12
1.14
2.14
2.14
2.14
2.14
2.14
2.14
2.14
3.14
3.14
3.14
3.14
3.14
3.14
3.14
1.14
2.14
2.14
2.14
2.14
2.14
5/23/2012
5/23/2012
5/23/2012
5/23/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/19/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
145
139
136
138
174
157
175
173
172
171
152
156
159
155
170
161
160
151
154
158
167
162
166
164
168
169
165
163
153
199
193
176
186
185
184
194
198
187
189
179
191
195
177
0.796
0.434
0.331
0.363
0.6849
0.6001
1.1991
1.7987
1.4161
1.1313
1.5028
1.7181
1.9229
2.304
2.425
2.293
3.2555
3.4947
3.8787
.
0.1111
0.0892
0.1636
0.3142
0.2888
0.291
0.2986
0.7008
0.9175
0.702
0.906
1.939
1.154
1.197
1.399
1.223
1.683
1.584
1.85
4.037
1.77
1.581
6.625
0.23
0.12
0.08
0.07
0.37
0.33
0.51
0.71
0.55
0.43
0.51
0.45
0.47
0.52
0.50
0.40
0.48
0.49
0.55
.
0.04
0.03
0.04
0.07
0.05
0.04
0.04
0.06
0.06
0.32
0.29
0.61
0.35
0.35
0.40
0.31
0.42
0.36
0.38
0.74
0.32
0.28
0.41
96
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
.
.
.
.
immature
.
.
regenerating
.
.
.
.
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
.
.
.
.
.
.
.
.
.
.
.
.
regenerating
regenerating
regenerating
regenerating
.
regenerating
regenerating
.
regenerating
regenerating
regenerating
regenerating
2.14
2.14
2.14
2.14
1.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
3.22
2.22
3.22
3.22
1.22
2.22
2.22
2.22
2.22
2.22
3.22
3.22
5.22
6.22
1.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
2.22
3.22
5.22
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
6/21/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/10/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
192
190
197
200
180
181
196
188
178
182
183
4228
4222
4238
4237
4234
4218
4223
4224
4236
4219
4225
4231
4235
4220
4232
4240
4239
4229
4226
4241
4221
4242
4227
4233
4230
4251
4249
4253
4265
4244
4246
4247
0.042
0.085
0.04
0.127
0.198
0.198
0.086
0.108
.
0.121
0.084
0.029
0.342
0.707
0.67
0.845
0.991
1.354
1.469
1.491
1.667
1.825
1.919
2.772
8.307
0.018
0.016
0.024
0.03
0.061
0.088
0.108
0.08
0.096
0.168
0.246
0.034
0.483
0.65
1.306
1.425
1.932
1.225
0.02
0.03
0.01
0.04
0.06
0.06
0.02
0.03
.
0.03
0.02
0.24
0.24
0.30
0.28
0.32
0.33
0.37
0.38
0.35
0.36
0.38
0.39
0.56
0.55
0.02
0.02
0.02
0.02
0.03
0.03
0.03
0.02
0.02
0.02
0.02
0.28
0.28
0.32
0.44
0.39
0.49
0.31
97
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
.
.
.
.
.
.
.
.
.
.
.
.
immature
.
.
immature
.
.
.
.
.
regenerating
regenerating
regenerating
regenerating
.
.
.
.
.
.
.
.
.
.
.
immature
immature
immature
.
regenerating
regenerating
regenerating
2.22
2.22
2.22
2.22
1.22
2.22
2.22
2.22
2.22
1.22
2.22
0.27
1.27
1.27
1.27
1.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
5.27
1.27
1.27
1.27
1.27
1.27
2.27
2.27
2.27
2.27
3.27
4.27
0.28
1.28
1.28
2.28
2.28
3.28
2.28
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/11/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/16/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
7/18/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
4259
4255
4263
4243
4257
4250
4262
4261
4248
4264
4256
4260
4258
4245
4252
4254
4274
4278
4267
4268
4270
4271
4273
4275
4266
4277
4280
4279
4269
4276
4272
4286
4296
4285
4292
4294
4281
4290
4287
4289
4284
4288
4293
1.803
2.414
4.712
5.728
8.629
0.035
0.057
0.056
0.1
0.104
0.081
0.102
0.126
0.175
0.111
0.321
0.682
0.441
0.557
0.897
0.894
2.181
2.175
2.455
5.453
0.036
0.078
0.2
0.098
0.136
0.679
0.419
0.621
0.551
0.64
1.215
0.717
1.181
0.96
0.013
0.078
0.048
0.104
0.42
0.42
0.42
0.37
0.55
0.02
0.02
0.02
0.03
0.03
0.02
0.03
0.03
0.04
0.02
0.03
0.50
0.32
0.32
0.38
0.36
0.52
0.47
0.39
0.45
0.02
0.03
0.05
0.02
0.02
0.05
0.28
0.30
0.26
0.29
0.43
0.25
0.32
0.24
0.01
0.03
0.02
0.03
98
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
f
f
f
f
f
f
f
f
m
m
m
m
.
regenerating
regenerating
regenerating
regenerating
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
regenerating
.
.
.
.
.
.
.
.
.
immature
regenerating
immature
.
regenerating
.
.
.
.
2.28
2.28
5.28
4.28
5.28
1.28
2.28
2.28
2.28
2.28
2.28
2.28
2.28
2.28
2.28
5.28
1.29
1.29
1.29
1.29
2.29
2.29
2.29
3.29
5.29
1.29
1.29
2.29
2.29
3.29
9.29
1.30
1.30
1.30
1.30
2.30
1.30
2.30
2.30
1.30
2.30
1.30
2.30
7/18/2012
7/18/2012
7/18/2012
7/18/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/13/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
4283
4291
4282
4295
224
223
211
225
221
218
222
210
219
204
209
207
215
202
201
220
213
212
216
217
206
208
214
205
203
250
247
249
242
243
240
246
244
236
238
233
234
229
237
0.062
0.074
0.101
0.225
0.684
1.395
1.421
1.626
1.22
1.722
1.599
1.42
1.302
2.317
1.673
1.856
2.951
4.49
5.697
0.124
0.121
0.086
0.066
0.103
0.11
0.099
0.176
0.365
0.479
0.731
0.843
0.778
0.665
1.032
1.111
1.988
2.75
2.312
3.211
3.306
2.757
4.208
3.62
0.02
0.02
0.02
0.03
0.31
0.47
0.40
0.45
0.33
0.44
0.38
0.33
0.30
0.51
0.33
0.32
0.35
0.51
0.38
0.04
0.03
0.02
0.02
0.03
0.03
0.02
0.03
0.03
0.04
0.39
0.42
0.31
0.26
0.34
0.26
0.41
0.57
0.36
0.46
0.39
0.31
0.40
0.34
99
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
.
.
.
.
immature
.
regenerating
.
regenerating
regenerating
regenerating
regenerating
.
regenerating
.
regenerating
.
.
regenerating
.
.
.
.
.
.
.
.
.
.
regenerating
.
.
.
.
regenerating
regenerating
regenerating
regenerating
.
regenerating
regenerating
.
regenerating
1.30
2.30
2.30
2.30
1.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
3.37
4.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
4.37
5.37
2.37
2.37
2.37
2.37
2.37
2.37
2.37
3.37
2.37
2.37
2.37
2.37
3.37
3.37
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
8/14/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
9/18/2012
10/9/2012
10/9/2012
10/9/2012
10/9/2012
10/11/2012
10/11/2012
10/16/2012
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
Cat
226
245
248
232
239
228
235
230
241
227
231
274
273
275
272
268
264
269
263
259
260
256
252
253
251
254
271
266
262
265
270
267
255
257
261
258
4298
4299
4297
4300
4302
4301
4303
6.918
0.059
0.069
0.057
0.066
0.119
0.191
0.143
0.177
0.199
0.239
0.625
.
0.724
0.906
1.147
1.084
1.87
1.313
1.297
1.432
2.6
5.52
4.173
9.375
8.214
0.063
0.057
0.047
0.091
0.132
0.156
0.082
.
0.323
.
1.941
0.167
0.541
0.37
0.316
0.442
0.45
0.42
0.03
0.03
0.02
0.02
0.02
0.04
0.03
0.03
0.03
0.03
0.36
.
0.31
0.38
0.42
0.36
0.58
0.37
0.35
0.33
0.34
0.59
0.39
0.61
0.50
0.02
0.02
0.02
0.03
0.04
0.04
0.02
.
0.02
.
0.46
0.04
0.12
0.08
0.08
0.10
0.11
100
f
m
m
m
m
m
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
m
m
m
f
m
m
m
m
m
m
regenerating
.
.
.
.
.
.
.
.
.
.
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
regenerating
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
regenerating
.
.
.
.
.
.
5.37
1.37
2.37
1.37
2.37
2.37
2.37
3.37
2.37
2.37
3.37
1.47
1.47
1.47
2.47
2.47
2.47
2.47
2.47
2.47
2.47
3.47
5.47
4.47
4.47
5.47
2.47
2.47
2.47
2.47
1.47
2.47
2.47
2.47
5.47
6.47
2.52
2.52
2.52
2.52
2.53
2.53
2.54
10/18/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
10/25/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/15/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
11/20/2012
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
4304
291
284
290
288
289
286
281
287
285
283
276
280
279
277
282
278
292
297
295
293
296
294
298
317
322
321
316
309
315
318
308
311
307
310
305
304
301
320
319
300
312
302
0.714
0.934
1.487
1.784
1.644
2.241
2.219
3.596
2.872
10
0.455
0.521
0.849
0.412
0.621
0.666
3.351
2.147
4.054
0.463
0.597
1.086
1.453
1.686
0.941
1.413
1.984
2.321
2.338
2.89
3.095
3.388
3.543
3.15
2.578
3.253
7.564
0.322
0.63
0.543
0.637
0.618
0.764
0.13
0.51
0.68
0.59
0.53
0.70
0.58
0.66
0.48
0.78
0.11
0.12
0.18
0.08
0.12
0.09
0.20
0.86
0.98
0.22
0.20
0.30
0.33
0.25
0.47
0.64
0.83
0.85
0.81
0.93
0.91
0.99
0.91
0.81
0.63
0.62
0.92
0.16
0.29
0.19
0.22
0.20
0.24
101
m
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
m
f
f
m
m
m
m
m
f
f
f
f
f
f
f
f
f
f
f
f
f
m
m
m
m
m
m
.
developing
developing
developing
developing
developing
developing
developing
developing
developing
.
.
.
.
.
.
.
developing
developing
.
.
.
.
.
developing
developing
developing
developing
developing
developing
developing
.
.
.
developing
.
developing
.
.
.
.
.
.
2.55
1.57
2.57
2.57
1.57
2.57
2.57
2.57
2.57
3.57
2.57
2.57
2.57
2.57
3.57
2.57
4.57
1.62
2.62
1.62
2.62
2.62
2.62
2.62
1.64
1.64
1.64
1.64
2.64
2.64
2.64
1.64
1.64
2.64
2.64
2.64
3.64
1.64
2.64
2.64
2.64
1.64
2.64
11/20/2012
11/20/2012
11/20/2012
11/20/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
12/19/2012
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/23/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
1/24/2013
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
303
314
306
313
338
335
339
334
336
328
329
341
340
337
333
331
332
330
326
325
324
327
323
347
346
344
343
4305
348
342
345
351
350
4307
352
4306
349
0.9
1.076
1.415
1.187
2.459
5.351
.
6.276
5.306
13.039
13.454
0.61
0.809
0.603
0.804
0.851
1.839
1.136
5
0.61
0.642
0.844
2.079
2.989
2.696
6.918
8.137
23.526
0.446
0.948
1.667
17.591
14.114
26.127
29.753
1.497
1.427
0.21
0.21
0.28
0.23
1.22
2.52
.
1.76
1.38
2.68
2.15
0.26
0.29
0.21
0.25
0.25
0.51
0.28
1.57
0.25
0.23
0.27
0.44
1.31
1.10
2.39
2.12
4.37
0.17
0.28
0.40
4.23
1.92
3.56
4.00
0.36
0.33
102
m
m
m
m
f
f
f
f
f
f
f
m
m
m
m
m
m
m
f
m
m
m
m
f
f
f
f
f
m
m
m
f
f
f
f
m
m
.
.
.
.
SC
SC
developing
SC
SC
SC
SC
.
.
.
.
.
.
.
SC
.
.
.
.
developing
developing
SC
SC
SC
.
.
.
SC
SC
.
SC
.
.
1.64
2.64
3.64
2.64
1.72
1.72
1.72
2.72
2.72
1.72
3.72
1.72
1.72
1.72
2.72
2.72
2.72
2.72
1.72
1.72
1.72
1.72
2.72
1.81
1.81
1.81
1.81
1.81
1.81
1.81
1.81
1.82
1.82
2.82
1.82
1.82
1.82
APPENDIX III
Appendix III. Season, Lake, Plot sampled, and number of largemouth bass collected in
each plot for plots sampled during seasonal sampling.
Season
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Lake
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Plot
46
68
64
72
44
54
55
57
58
62
61
66
6
14
20
32
34
8
11
25
26
29
39
41
61
63
64
65
66
62
45
48
49
51
55
59
# Bass/ 600
Seconds
4
3
11
3
4
15
14
5
2
7
1
7
4
6
9
0
5
1
1
12
12
4
6
8
12
1
8
2
8
1
1
2
0
31
2
3
103
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Winter
Winter
Winter
Winter
Winter
Winter
Winter
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
6
4
3
28
10
18
23
25
28
35
39
42
64
65
67
69
71
72
44
46
51
57
58
59
10
13
17
19
21
26
2
5
38
39
40
41
45
47
51
52
54
57
60
9
3
10
28
3
7
3
3
3
3
3
4
0
0
2
0
0
2
0
0
2
0
0
0
0
0
0
1
0
1
1
0
0
0
0
0
0
0
0
0
0
0
0
104
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Cataouatche
Cataouatche
Cataouatche
Cataouatche
Cataouatche
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
des Allemands
62
66
68
72
75
43
42
39
37
36
35
33
25
14
12
6
3
0
1
0
0
0
0
0
0
0
0
0
1
0
1
0
0
0
105
APPENDIX IV
Appendix IV. Sampling Season, Lake, Fish ID, Unidentified (Un), Detritus (Det), Fish,
Shrimp, Crabs, Insects, Crawfish, and Total diet items found in the stomachs of
largemouth bass collected in the Lac des Allemands and Lake Cataouatche.
Season
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Lake
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Fish
ID
4075
4102
4106
4118
4120
4147
4154
4215
4073
4074
4103
4104
4108
4110
4111
4121
4148
4155
4167
4149
4162
4163
4200
4203
4214
4217
4107
4119
4122
4151
4152
4072
4105
4112
4153
Un
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
1
0
0
0
0
1
0
0
0
1
0
0
0
0
Det
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
Fish
0
0
0
0
0
0
0
0
1
1
0
0
1
0
0
0
0
0
0
1
2
0
0
0
2
1
2
3
3
3
1
4
4
4
2
106
Shrimp Crab Insects Crayfish
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
1
0
0
0
0
0
0
0
1
0
0
0
1
0
0
0
1
0
0
0
0
0
0
0
1
0
0
0
0
2
1
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
0
Total
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
3
3
3
3
3
4
4
4
4
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
4109
4150
4156
4161
4201
4157
4169
4166
4202
4216
4113
4165
4198
4199
4159
4158
4160
4168
4164
4117
4116
4123
4132
4135
4182
4188
4190
4195
4205
4208
4126
4131
4172
4185
4186
4189
4191
4192
4193
4194
4125
4127
4137
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
5
5
4
5
3
8
7
4
9
9
11
11
11
11
12
13
12
13
15
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
0
1
0
2
1
107
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
54
63
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
4
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
2
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
5
5
5
5
5
8
8
9
9
10
11
11
11
11
12
13
13
13
15
54
63
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
2
2
2
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Spring
Summer
Summer
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
4140
4141
4170
4173
4174
4183
4184
4197
4211
4212
4213
4115
4142
4171
4180
4196
4207
4129
4133
4145
4176
4206
4209
4210
4138
4144
4187
4130
4134
4124
4146
4204
4179
4128
4178
4139
4136
4175
4177
4181
4143
4288
4284
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
0
0
1
0
0
0
0
0
0
0
0
0
2
2
2
1
1
2
1
1
2
2
2
3
3
2
1
2
3
0
1
0
0
4
4
1
0
1
1
3
6
0
2
3
0
12
0
1
1
0
4
0
0
0
0
108
0
0
0
0
0
0
1
0
0
0
0
0
0
1
2
1
0
4
1
0
0
0
0
0
5
3
0
3
0
6
6
0
12
0
1
7
0
26
0
34
68
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
2
4
4
0
0
3
0
0
4
0
0
0
0
7
0
0
12
6
19
0
26
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
2
2
2
2
2
2
2
2
2
2
3
3
3
3
3
3
4
4
4
4
4
4
4
5
5
5
6
7
8
8
10
12
13
13
14
20
26
30
34
68
0
0
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
4282
4283
4227
4235
4236
4231
4238
4232
4226
4230
4223
4220
4289
4293
4294
4291
4295
4281
4285
4233
4234
4239
4242
4224
4286
4290
4292
4296
4225
4221
4229
4237
4241
4222
4287
4240
4219
4218
4258
4262
4252
4254
4245
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
0
1
1
0
1
1
0
1
1
0
1
1
0
2
2
2
1
0
0
2
0
3
3
2
1
1
0
1
0
0
0
0
0
109
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
2
4
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
1
3
0
3
0
0
0
0
4
7
9
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
3
3
3
3
3
4
5
5
7
10
0
0
0
0
0
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Summer
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
Fall
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
4249
4248
4266
4272
4267
4275
4264
4263
4261
4259
4257
4255
4246
4243
4269
4271
4273
4277
4276
4278
4256
4250
4244
4247
4268
4279
4253
4270
4260
4265
4280
4274
4303
4297
4298
4299
4300
295
4301
4304
292
293
294
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
0
1
0
1
1
1
1
1
1
1
2
2
2
2
0
2
1
1
0
2
0
0
1
0
0
0
1
1
1
1
0
1
0
1
0
1
110
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
2
3
3
23
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
2
0
1
0
3
0
0
1
0
15
59
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
3
3
3
4
4
38
59
0
1
1
1
1
0
1
1
1
1
1
Fall
Fall
Fall
Fall
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
Winter
DA
DA
DA
DA
Cat
Cat
Cat
Cat
Cat
Cat
Cat
Cat
DA
DA
DA
DA
DA
DA
296
297
298
4302
347
346
345
4305
348
342
343
344
349
350
352
4306
351
4307
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
1
1
1
1
1
0
0
0
0
1
0
1
0
0
0
1
9
111
0
0
0
0
0
0
1
0
2
4
3
7
0
0
0
0
3
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
2
0
0
0
2
0
0
0
0
0
0
0
5
2
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
1
0
0
0
1
1
1
3
1
1
1
2
2
4
4
7
1
1
1
5
6
9
BIOGRAPHICAL SKETCH
Bo Anthony Boudreaux was born on 6 July 1989, in Thibodaux, Louisiana. After
graduating as Valedictorian from Thibodaux High School in Thibodaux, Louisiana, in
2007, Bo attended Nicholls State University where he earned a Bachelor of Science in
Marine Biology and a minor in Chemistry in 2011. In the fall of 2011, Bo enrolled in the
Marine and Environmental Biology graduate program at Nicholls State University, in
Thibodaux, Louisiana. Bo conducted research on the largemouth bass in the upper
Barataria Estuary. While at Nicholls State, Bo assisted in coastal restoration and cleanup, Thibodaux wetland assimilation project, bird banding and recapture projects,
freshwater turtle assemblage project, raptor project, and interned with Coastal Estuary
Services and Louisiana Department of Wildlife and Fisheries.
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CURRICULUM VITAE
Bo Boudreaux
171 Belle Terre Dr.
Telephone: 985-438-5133
Thibodaux, LA 70301
Email: bboudreaux9@its.nicholls.edu
Education:
2007 – 2011, Major: Marine Biology, Minor: Chemistry, Nicholls State
University.
2011- Current, Masters: Marine and Environmental, Nicholls State
University.
Research Experience:
2008-2009
Research Assistant: Effects of artificial perches on raptors
and small mammals, Supervisor: Dr. Aaron Pierce.
Conducted raptor point count surveys and small mammal
trapping/tagging.
Summer 2009
Research Assistant: Supervisor: Dr. Aaron Pierce and Dr.
Earl Melancon. Conducted water quality monitoring, oyster
recruitment surveys and mark and recapture of breeding
waterbirds on barrier islands.
2009-2010
Research Assistant: Freshwater turtle assemblage and leech
parasitism in canal habitats, Supervisor: Dr. Aaron Pierce.
Conducted turtle trapping/mark/recapture, leech collection
and identification, water quality monitoring and aquatic
vegetation surveys.
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Summer 2010
Research Assistant: Supervisor: Dr. Aaron Pierce and Dr.
Earl Melancon. Conducted water quality monitoring, oyster
recruitment surveys and mark and recapture of breeding
waterbirds on barrier islands.
2008-2011
Research Assistant: Wetland Assimilation Monitoring,
Thibodaux wastewater wetlands. Supervisor: Dr. Aaron
Pierce Conducted water quality monitoring, leaf litter
accumulation, soil sampling, forest inventory, and
understory vegetation surveys.
Fall 2011- Current
Research Assistant: Supervisor: Dr. Quenton Fontenot
Effects of a freshwater diversion on a Largemouth Bass
population. Compare age and growth, gonad histology and
stomach contents of largemouth bass collected from Lac
des Allemands and Lake Cataouatche
Other Work Experience:
2005-2007
Helper/ Welder @ ABL Fabricators: Helped to build
barges, offshore production platform legs, and various
others metal works. Worked here through high school.
Summer 2007
Worked at the Lafourche Parish School Board Printing
Department: Ran print presses, copiers, hydraulic cutters,
and drilling-hole presses
2005-2011
Commercial Fisherman: When time allowed, I
commercially fished for catfish, bowfin, garfish, crawfish,
turtles, and/or frogs
Summer 2011
Biologist intern @ Shaw/ Coastal Estuary Services
division: Conducted water quality sampling, vegetation
surveys and wetland delineations
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Summer 2012
Biologist intern @ Louisiana Department of Wildlife and
Fisheries Marine division: Collected monthly fish and
oyster samples by means of gillnetting, trawling, and oyster
dredging.
Volunteer Work:
Bay Jimmy restoration project
Beach Sweep at Elmer’s Island
Mangrove planting on Last Island Summer 2011
Mangrove planting on Last Island Summer 2012
Publications:
Boudreaux, B.A. and A.R. Pierce. In Review. The effects of artificial perches on
wintering raptors and small mammal populations. Journal of Louisiana Academy
of Science.
Boudreaux, B.A. and A.R. Pierce. In Review. Freshwater turtle assemblage and leech
parasitism in artificial canal habitats. Southeastern Naturalist.
Presentations:
Boudreaux, B.A. and A.R. Pierce. 2010. The effects of artificial perches on wintering
raptors. The 84th Annual Meeting of the Louisiana Academy of Sciences,
Alexandria, Louisiana.
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Boudreaux, B.A. and A.R. Pierce. 2011 (Poster). Freshwater turtle assemblage and leech
parasitism in artificial canal habitats. Louisiana Association of Professional
Biologists/Louisiana Chapter, The Wildlife Society Fall Symposium, Lafayette,
Louisiana.
Boudreaux, B.A. and Q. Fontenot. 2013 (Poster). Comparison of two Largemouth bass
populations in the Upper Barataria Estuary. Nicholls State University research
week, Thibodaux, Louisiana
Awards:
2007
Thibodaux High School Valedictorian and Top Scholar Award
2010
Dr. James G. Ragan Marine Biology Service Award
2011
The Deanna Bonvillain Award for Outstanding Student Service
2013
1st Place in poster competition/ Nicholls State University, 2013 research
week
Other Skills: Advanced level boating skills, and various methods of fishing including
electrofishing, gillnetting, hoopnetting and trawling; Intermediate level skills in
Microsoft Word, Excel, and Power Point. Intermediate level skills using SAS software.
Experienced in small mammal trapping, identification, and tagging; avian surveys,
identification, and mark/recapture methods; vegetation surveys, composition and
abundance of herbaceous and woody species, diameter at breast height measurements,
substrate collections, leaf litter collection, dry biomass measurements, and wetland
delineations; use of auto level; water quality measurements including YSI meter readings
and sample collection methods. Removing otiloths. aging fish, and interpreting gonad
histology slides.
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