08/2015 Rhonda Ann Patterson Curriculum Vitae Address Biology Department Ogden College of Science & Engineering Western Kentucky University 1906 College Heights Blvd. #11080 Bowling Green, KY 42101-1080 270-745-4978 or 270-745-2351 (phone) rhonda.patterson@wku.edu Born Carthage, Missouri Education B.S. Ag. (1987) in Animal Science from University of Missouri-Columbia; Columbia, MO Ph.D. (1998) in Immunology from University of Southern Mississippi; Hattiesburg, MS Fellowships Patricia Roberts Harris Fellowship Honors Who’s Who Among America’s Teachers (2004 and 2005) Professional Society Activities International Association of Aquatic Animal Medicine Past-President (2006-2007)Responsible for serving on the “Nominations and Awards” committee (this year and the next two) as well as one final year on the Executive Board. Responsible for filling in for the President if for any reason he is not able to fulfill his duties. President (2005-2006) Responsible for overseeing all of the activities of the organization. Run all Executive Board meetings and the annual business meeting. Write all official correspondence for the organization requiring presidential seal. President-Elect (2004-2005) Responsible for planning scientific program and coordinating with host institution for 36th Annual Conference of the International Association for Aquatic Animal Medicine, May, 2005, Seward, Alaska. Newsletter Editor and Executive Board member - starting fall, 2000. Chair Pre-Conference all day workshop on "Immunology for the Aquatic Animal Clinician" at the 34th Annual Conference of the International Association for Aquatic Animal Medicine, May, 2003, Waikoloa, Hawaii. Associate Editor Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, September 2000, New Orleans, LA. Chairperson (1999-2001) Conservation and Governmental Affairs Committee of the International Association of Aquatic Animal Medicine. Co-chair Pre-Conference “Marine Mammal Immunology Workshop for Clinicians” at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Member 1989 - present Society for Marine Mammalogy (Member 1989-2006) International Society of Developmental and Comparative Immunology (Member 2000 2008) American Association for Women in Community Colleges (Member 2007-2009) Association of College & University Biology Educators (Member 2007-present) National Association of Biology Teachers (Member 2007-present) The National Association for Developmental Education (Member 2007) National Science Teachers Association (Member 2012 - present) Society Memberships Associated with Profession Defenders of Wildlife Environmental Defense Fund National Parks Conservation Association National Wildlife Federation Leaders Club Kentucky Goat and Sheep Association Professional Experience August 2005 – present: Assistant Professor, Western Kentucky University, 1906 College Heights Blvd., Bowling Green, KY 42101 July 2014 – present: Biology Department, Ogden College of Science & Engineering July 2010 – June 2010: Liberal Arts & Sciences Department, University College August 2005 – June 2010: Liberal Arts & Sciences Division, Bowling Green Community College August 2004 – 2009: President of Animal Immunodiagnostics, Reagents & Research, LLC, Brownsville, KY 42210 August 1988 – August 2006: University of Southern Mississippi, Department of Biological Sciences, 118 College Drive, Hattiesburg, MS 39406 May 2001 – August 2006: Research Scientist I and part-time instructor May 1998 – May 2001: Postdoctoral Research Associate June – August 1995 and 1996: Instructor June 1997 – May 1998: Instructor August 1988 – August 1997: Teaching assistant August 1995: Graduate assistant, The University of Southern Mississippi's Center for Molecular and Cellular Biosciences' Undergraduate Faculty Enhancement Workshop #4: Protein Chemistry and Immunochemistry. Funded by National Science Foundation's Undergraduate Faculty Enhancement Program Courses Taught General Biology for Non-biology Majors - lecture and laboratory Introduction to Biology (regular and honors) - lecture and laboratory General Microbiology - lecture and laboratory Immunology and Serology - lecture and laboratory Immunochemistry - lecture and laboratory Viral Pathogenesis and Diagnosis - lecture Additional Teaching not in formal course Research methods both in the laboratory and in the literature were taught to undergraduate students both at Western Kentucky University and University of Southern Mississippi. At USM there was a course that students could sign up for credit for this work. At WKU there was not such a course; however, eight separate students have performed this work without receiving academic credit (simply to have the experience). Western Kentucky University (UC) and College Committees (CC) or University Organizations and College Organizations (UO or CO) Advising Task Force Committee (CC) - 2007 to 2011 Christian Faculty and Staff Fellowship (UO) - 2005 to 2008 Community College 20th Birthday Party Planning Committee - January to August 2006 Faculty Search Committee (CC) Geography position - fall 2005 and spring 2006 History position - spring 2007 Research & Creative Activities Program (UC) - college alt (2006 - 2008), full member (2009-2014) Science Awareness Society (CO) - co-chair 2005 to 2011 Student Travel Award Committee (CC) - 2005 to 2011 Liberal Arts and Sciences Division Travel Award Committee (CC) - 2008 to 2011 Student Engagement Expo (CC) - co-chair 2008 to 2010 Bowling Green Community College Planning Council (CC) - member 2007 to 2009 Cornerstone Committee (CC) - member 2012 Search Committee for Director, Student Support Programs (CC) - member July to December 2013 South Campus Academic Network Committee (CC) - member 2012 to present University College Sabbatical Leave Committee (CC) – member October to November 2012 Continuance Committee for Dr. James Asare (CC) – chairperson September 2013 Thompson Complex North Wing Building Committee (CC) – member October 2014 to present Colonnade Assessment Committee (CC) – member November to December 2014 Instructor Search Committee (CC) – member November 2014 to present University Southern Mississippi Committees Member of Institutional Animal Care and Use Committee Member of two ad hoc committees responsible for rewriting the "Standard Operating Procedures for the Animal Research Facility" and writing a facility improvement grant Associate member of graduate faculty Served as member on five doctoral and two master's committees Mentor for the McNair Scholars Program Advisor for the Presbyterian Fellowship group Professional Services Reviewer, Textbook. MacMillan Publisher. 2014. MacMillan Publishers contacted me and asked if I would review a chapter in Shuster’s “Biology for a Changing World, 3e”. This entailed reading through the table of contents, picking one of several chapters, reading one of the chapters, answering multiple questions on both the table of contents and the chapter read. Reviewer, Journal Article. Aquatic Mammals. 2015. Dr. Kathleen M. Dudzinski (managing editor) asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer, Textbook. Pearson Publisher. 2014. Pearson Publishers contacted me and asked me to review Cowan/Bunn's “Microbiology Fundamentals or Cowan's Microbiology: A Systems Approach 3e”. This entailed filling out an extensive survey to detail my opinions on various aspects of the book, especially as it compared to my current text. Reviewer, Lab Exercise in Non-Majors Biology Lab Manual, Whiley-Freeman Publishing, 2014. A representative from Whiley-Freeman Publishing contacted me regarding performing a review of one of their new virtual lab experiments for their nonmajors lab manual. All of the information was provided to me along with an extensive review form to fill out and return to them. Reviewer, Journal Article. Aquatic Mammals. 2014. Drs. Kathleen M. Dudzinski, Justin Gregg, and Robin Paulos (managing editors) asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer, Journal Article. Aquatic Mammals. 2010. Drs. Kathleen M. Dudzinski, Justin Gregg, and Robin Paulos (managing editors) asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer of Conference Abstracts and Session Chair. International Association for Aquatic Animal Medicine. 2009. The abstracts that were submitted for the immunology sessions were forwarded from the program chair to me. My job was to review and edit the abstracts for content and format. When I was finished I returned them to the program chair for submission in the conference proceedings. During the conference I was in charge of the session: introducing the talks and keeping the sessions running on time. Reviewer, Journal Article. Aquatic Mammals. 2009. Dr. Jeanette Thomas, managing editor, asked me to review an article submission for the journal "Aquatic Mammals". This is the oldest peer-reviewed journal in the marine mammal field. Reviewer, Journal Articles. Developmental and Comparative Immunology. 2008. Dr. Gregory W. Warr, Co-Editor-in-Chief of the journal Developmental & Comparative Immunology, asked me to review four different article submissions. This journal is an internationally known journal within the field. Professional Development Attendee at PKAL Summer Leadership Institute, July 2015, sponsored by AAC&U. The PKAL Summer Leadership Institute is designed for both early and mid-career STEM faculty engaged in leading projects aimed at transforming undergraduate STEM education in their classrooms, departments, and institutions. The five-day intensive Institute provides faculty participants with the theory and practice required to effectively manage the politics of such change and contribute to the national STEM higher education reform effort. Book Chapters Middlebrooks, B. L. and R. A. Patterson. August, 2002. Methods for purification and study of cetacean immunoglobulins. In Cell and molecular biology of marine mammals. C. J. Pfeiffer ed. Krieger Publishing Co., Inc. Patterson, R. A., B. L. Middlebrooks, and J. C. Jones. August, 2002. Application of ELISA methodology for detection of Erysipelothrix rhusiopathiae antibody titers in cetaceans. In Cell and molecular biology of marine mammals. C. J. Pfeiffer ed. Krieger Publishing Co., Inc. Dissertation Patterson, R.A. 1998. An analysis of the humoral immune system of three species of cetaceans: The Atlantic bottlenose dolphin (Tursiops truncatus), beluga whale (Delphinapterus leucas), and Pacific white-sided dolphin (Lagenorhynchus obliquidens). The University of Southern Mississippi, Hattiesburg, MS. Refereed Publication Heise, R.J., Bringolf, R.B., Patterson, R.A., Cope, W.G., Ross, S.T. (2009). Plasma vitellogenin and estradiol of adult Gulf sturgeon (Acipenser oxyrinchus desotoi) in the Pascagoula River Basin (MS, USA). Transactions of the American Fisheries Society, 138:1028-1035. Jones, J. C., R. A. Patterson, and B. L. Middlebrooks. 2000. Evaluation and refinement of an ELISA assay designed to detect antibodies against Erysipelothrix rhusiopathiae in cetaceans. Infectious Disease Review. 2(4):218-222. R.A. Patterson. 2000. Congress Report International Workshop on Erysipelas in Cetaceans. Infectious Disease Review. 2(4):225-226. Non-refereed Publications Patterson, R. A., J. C. Jones, and B. L. Middlebrooks. 1995. Development of ELISA methodologies to evaluate anti-Erysipelothrix rhusiopathiae titers in several cetacean species. IAAAM News 26(3):6-7. Published Abstracts and Presentations Patterson, R. A. “Students Use Classroom Topics to Produce Brochures with International Emphasis”. Engaging the Spirit: Celebrating Student Success. WKU, Bowling Green, KY. August 18, 2008. Patterson, R. A. “Mentoring Students and Colleagues: Step Up and Reach Out”. 2008 Annual Conference of the Kentucky Chapter of the American Association for Women in Community Colleges, Kentucky Chapter of the American Association for Women in Community Colleges, Lexington, KY. February 28, 2008. Jensson, Laura and Rhonda Patterson. “Note Taking and Test Studying Tips” seminar given at the Bowling Green Community College of Western Kentucky University, Bowling Green, KY. Sept, 2006; Feb, 2007; Sept, 2007; Feb, 2008; Sept, 2008; Feb, 2009; Sept, 2010; and Mar, 2010. Patterson, Rhonda. “Immunology of Marine Mammals: What Do We Know?”, invited speaker at the John G. Shedd Aquarium, Chicago, IL. August 8, 2006. Coleman, Amaziah, Bobby Middlebrooks, and Rhonda Patterson. Antibody Titers in Four Different Populations of Atlantic Bottlenose Dolphins (Tursiops truncatus) Serum Samples to Erysipelothrix rhusiopathiae. Proceedings of the 37th Annual Conference of the International Association for Aquatic Animal Medicine, 37:86, 2006. Boehm, Jeffrey and Rhonda Patterson. Erysipelas in Cetaceans: A Coordinated Research Program. Proceedings of the 16th Biennial Conference of the Society for Marine Mammalogy, p38, 2005. Estepp, Jessica D., Bobby L. Middlebrooks, and Rhonda A. Patterson. Development of an Immunodiagnostic Assay for Brucella sp. in the Harp Seal (Phoca groenlandica) and Hooded Seal (Cystophora cristata). Proceedings of the 36th Annual Conference of the International Association for Aquatic Animal Medicine, 36:97, 2005. Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. Development of an Enzyme-linked Immunosorbant Assay (ELISA) Using Anti-human IgA Reagents to Determine IgA Levels in the Serum of Several Individuals of the Harbor Seal, Phoca vitulina. Proceedings of the 36th Annual Conference of the International Association for Aquatic Animal Medicine, 36:96, 2005. Colvocoresses, Jennifer A., Bobby L. Middlebrooks, and Rhonda A. Patterson. Analysis of Steller Sea Lion IgG and IgM Levels Across the Geographic Range of the Population. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:99, 2004. Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. An Investigation of the Cross Reactivity of Antibodies Against the Immunoglobulins of Several Mammalian Species with Immunoglobulins of the Harbor Seal, Phoca vitulina. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:118-119, 2004. Frank, Jill M., Bobby L. Middlebrooks, and Rhonda A. Patterson. Characterization of Immunoglobulin G (IgG) in the Spectacled Eider, Somateria fischeri. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:133, 2004. Patterson, Rhonda A., Nicole A. Lilly, and Bobby L. Middlebrooks. IgG and IgA Concentrations Determined in Serum and Milk from Atlantic Bottlenose Dolphins (Tursiops truncatus) with Monoclonal Antibodies Specific for the Immunoglobulin Isotypes. Proceedings of the 35th Annual Conference of the International Association for Aquatic Animal Medicine, 35:134, 2004. Woodford, Jennifer G., Bobby L. Middlebrooks, and Rhonda A. Patterson. Preliminary study of the isolation and characterization of the immunoglobulins of the harbor seal, Phoca vitulina. Proceedings of the 34th Annual Conference of the International Association for Aquatic Animal Medicine, 34:70, 2003. Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Cloning and expression of the gene sequence of a 66kD surface protein of Erysipelothrix rhusiopathiae. Proceedings of the 33rd Annual Conference of the International Association for Aquatic Animal Medicine, 33:7, 2002. Lilly, Nicole A., Rhonda A. Patterson, and Bobby L. Middlebrooks. Development of a monoclonal antibody against Tursiops truncatus IgG for use as a reagent in a diagnostic ELISA. Proceedings of the 33rd Annual Conference of the International Association for Aquatic Animal Medicine, 33:8, 2002. Colvocoresses, Jennifer, Bobby Middlebrooks, and Rhonda Patterson. Evaluation of Canine and Human Antisera as Diagnostic Reagents for Use in the Identification of Immunoglobulins of Steller Sea Lions (Eumetopias jubatus) and Harbor Seals (Phoca vitulina). Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:94, 2001. Thames, Rachel, Jennifer Woodford, Rhonda Patterson, Frank Moore, Stan Kuczai, and M.A. Solangi. Effects of Illness, Associated Medicines, Age and Time of Day on Diurnal and Nocturnal Behavior of Captive Bottlenose Dolphins (Tursiops truncatus). Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:98, 2001. Boehm, Jeffrey, R., Geraldine Lacave, and Rhonda Patterson. Advancing the Study and Control of Erysipelas in Cetaceans. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:101-103, 2001. Jones, John C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Comparison of a Developmental ELISA for Anti-Erysipelothrix rhusiopathiae Antibodies with a Passive Agglutination Assay Using Latex Beads Coated with a Purified 64 kDa E. rhusiopathiae Specific Protein. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:108, 2001. Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. The Use of Molecular, Immunological and Microbiological Methods to Evaluate Similarities and Differences Between ATCC Reference Strains of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum and Environmental Isolates of Erysipelothrix rhusiopathiae Implicated in the Death of Marine Mammals. Proceedings of the 32nd Annual Conference of the International Association for Aquatic Animal Medicine, 32:109, 2001. Patterson, Rhonda A. What's New in Erysipelothrix rhusiopathiae Research in Marine Mammals? Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 210, 2000. Colvocoresses, Jennifer, Rhonda A. Patterson, and Bobby L. Middlebrooks. Purification of Immunoglobulin Isotypes G, A, and M from the Serum of the Steller Sea Lion (Eumetopias jubatus). Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 477, 2000. Jones, John C., Rhonda A. Patterson, and Bobby L. Middlebrooks. Use of a Mouse Model to Determine the Specificity of an ELISA Assay Designed to Detect Antibodies Against Erysipelothrix rhusiopathiae in Cetaceans. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 511, 2000. Middlebrooks, Bobby, L., Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Four Developmental Cell Lines from the Beluga Whale (Delphinapterus leucas) and the Pantropical Spotted Dolphin (Stenella attentuata) to Seal Herpes Virus and a Range of Mammalian Viruses from Several Families. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 526, 2000. Osgood, Robert C., Bobby L. Middlebrooks, and Rhonda A. Patterson. Characterization and Comparison of Five Isolates of Ersyipelothrix from Several Marine Mammal Sources. Proceedings of the Joint Conference of the American Association of Zoo Veterinarians and the International Association for Aquatic Animal Medicine, p. 541, 2000. Patterson, Rhonda, and Bobby Middlebrooks. Preparation of Antisera Specific for Major Immunoglobulin Isotypes (IgG, IgA, and IgM) of Three Species of Cetacean: Atlantic Bottlenose Dolphin (Tursiops truncatus), Pacific White-Sided Dolphin (Lagenorhynchus obliquidens), and the Beluga Whale (Delphinapterus leucas). Proceedings of the 13th Biennial Conference on the Biology of Marine Mammals, p 123, 1999. Middlebrooks, Bobby, Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Two Developmental Cell Lines from Tursiops truncatus to a Range of Mammalian Viruses. Proceedings of the 13th Biennial Conference on the Biology of Marine Mammals, p124, 1999. Patterson, Rhonda. “What Do We Know About the Immune Systems of Cetaceans and How Can We Apply It to Studying Steller Sea Lions?”, invited speaker at the Alaska SeaLife Center, Seward, Alaska, July 1, 1999. Patterson, Rhonda. “Dolphin Verses Dog Intelligence”, invited speaker for fifth graders at the Dixie Attendance Center, Dixie, Mississippi, March 27, 1999. Patterson, Rhonda. “Basics of the Immune System”, invited speaker for high school advanced placement students at Poplarville High School, Poplarville, Mississippi, September 30, 1999. Patterson, Rhonda. “Serology: Assays for determination of immunoglobulin levels and for determination of pathogen specific antibody titers”, Pre-Conference “Marine Mammal Immunology Workshop for Clinicians” at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Patterson, Rhonda. “Vaccines: Types, routes of exposure, and possible carriers”, PreConference “Marine Mammal Immunology Workshop for Clinicians” at the 30th Annual Conference of the International Association for Aquatic Animal Medicine, May, 1999, Boston, Massachusetts. Jones, John C., Rhonda A. Patterson, and Bobby L. Middlebrooks. The Antigenic Components of a Wild Strain of Erysipleothrix rhusiopathiae Determined by Immunostaining of Extracted Bacterial Surface Components with Serum from Tursiops truncatus, Lagenorhynchus obliquidens, and Delphinapterus leucas. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:20-21, 1999. Colvocoresses, Jennifer, Rhonda A. Patterson, and Bobby L. Middlebrooks. Preliminary Evaluation of Viral Susceptibility in Primary Kidney and Lung Cell Cultures of Tursiops truncatus. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:52, 1999. Walter, F. Lynn, Rhonda A. Patterson, and Bobby L. Middlebrooks. Preliminary Evaluation of the Kinetics of Changes in Lectin Levels Following Antigen Exposure as a Measure of Potential Disease Resistance in Strains of Penaeus vanammei. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:65-66, 1999. Osgood, Robert, Rhonda A. Patterson, and Bobby L. Middlebrooks. Application of Biochemical, Immunochemical, and Molecular Analysis to Comparison of Erysipelothrix rhusiopathiae Isolates from Two Species of Cetaceans to Each Other and to Strains Obtained from the American Type Culture Collection. Proceedings of the 30th Annual Conference of the International Association for Aquatic Animal Medicine, 30:82, 1999. Middlebrooks, Bobby, Jennifer Colvocoresses, and Rhonda A. Patterson. Susceptibility of Two Developmental Cell Lines from Tursiops truncatus to a Range of Mammalian Viruses. Abstracts of the 13th Biennial Conference on the Biology of Marine Mammals, p124, 1999. Patterson, Rhonda, and Bobby Middlebrooks. Preparation of Antisera Specific for Major Immunoglobulin Isotypes (IgG, IgA, and IgM) of Three Species of Cetacean: Atlantic Bottlenose Dolphin (Tursiops truncatus), Pacific White-Sided Dolphin (Lagenorhynchus obliquidens), and the Beluga Whale (Delphinapterus leucas). Abstracts of the 13th Biennial Conference on the Biology of Marine Mammals, p146, 1999. Patterson, Rhonda. “What Do We Know About the Immune Systems of Dolphins and Whales?”. Presented at the Biological Sciences Departmental Seminar Series, March 19, 1999. Patterson, R. A. and B. L. Middlebrooks. 1998. The jacalin affinity chromatography column proves to be an effective method for purifying IgA from Atlantic bottlenose dolphin (Tursiops truncatus), beluga whale, (Delphinapterus leucas), and Pacific whitesided dolphin (Lagenorhynchus obliquidens) serum. p. 103. In Proceedings of the 29th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A. and B. L. Middlebrooks. 1997. Evidence of immunoglobulins (IgG) in the milk of two cetacean species, Tursiops truncatus and Lagenorhynchus obliquidens. p. 78. In Proceedings of the 28th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A., J. C. Jones, and B. L. Middlebrooks. 1997. Comparison of species specific antisera and cross-reactive antisera in an ELISA for the determination of serum antibody titers against Erysipelothrix rhusiopathiae in Delphinapterus leucas and Lagenorhynchus obliquidens. p.79. In Proceedings of the 28th Annual International Association of Aquatic Animal Medicine Conference. Jones, J. C., R. A. Patterson, and B. L. Middlebrooks. 1996. Comparison of ELISA and agglutination titers against Erysipelothrix rhusiopathiae in cetaceans. p. 52. In Proceedings of the 27th Annual International Association of Aquatic Animal Medicine Conference. Jones, J. C., R. A. Patterson, and B. L. Middlebrooks. 1995. A biotin-avidin amplified ELISA for the detection of antibodies against Erysipelothrix rhusiopathiae in captive dolphins. p. 54. In Proceedings of the 26th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A. and B. L. Middlebrooks. 1995. Establishment of primary tissue culture lines of tissues from an Atlantic bottlenose dolphin (Tursiops truncatus) fetus. p. 55. In Proceedings of the 26th Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A., B. L. Middlebrooks, and M. A. Solangi. 1991. Effect of immunization schedule on immunoglobulin response in the Atlantic bottlenose dolphin (Tursiops truncatus). p. 1. In Proceedings of the 22nd Annual International Association of Aquatic Animal Medicine Conference. Patterson, R. A. 1990. Purification of immunoglobulins (IgG, IgA, and IgM) in the bottlenose dolphin (Tursiops truncatus). p. 172. In Proceedings of the 21st Annual International Association of Aquatic Animal Medicine Conference. Funded Research Proposals Title: Junior Faculty Scholarship Award Agency: WKU Office of Sponsored Programs Funds: $4000 (June 2007 to May 2008) Role: Responsible person for funds Title: Agency: Funds: Role: Teaching students about environmental sustainability and civic responsibility through plastic recycling at south campus WKU Provost (Provost Initiative in Excellence - PIE grant) $1333 (March to December 2007) Responsible person for funds Title: Agency: Funds: Role: Erysipelas in Cetaceans: A Coordinated Research Program The John G. Shedd Aquarium $20,000 (January to June 2007) Principal Investigator Title: Evaluation of humoral immune system parameters in Steller sea lions: preliminary evaluation of immunoglobulin isotypes found in low concentrations and continued reagent development Alaska SeaLife Center $30,000 (October 2004 to September 2005) Co-Investigator Agency: Funds: Role: Title: Agency: Funds: Role: Title: Agency: Funds: Role: Title: Agency: Funds: Role: Title: Agency: Funds: Role: Production of a DNA Vaccine Specific for the 64 kD Protective Antigen of Erysipelothrix rhusiopathiae Office of Naval Research $142,739 (ending August 2005) Co-Investigator Study to measure baseline levels of immunoglobulin classes in captive dolphins U.S. Department of Commerce/NMFS Institute for Marine Mammal Studies $74,969 (ended November 2003) Co-Investigator Investigation of the role of immunoglobulin classes and subclasses in humoral and mucosal immunity in cetaceans Office of Naval Research $397,054 (ended June 2005) Co-Investigator Development and Application of Reagents and Methodologies for Analysis of Comparative Immunoglobulin Levels in Harbor Seals. Alaska SeaLife Center $20,000 (ended September 2002) Co-Investigator Title: Agency: Funds: Role: Development and Evaluation of Monoclonal Antibodies for Analysis of Immunoglobulin Isotypes in Eiders (Including Spectacled and Steller Eiders) Alaska SeaLife Center $10,000 (ended September 2002) Co-Investigator Title: Agency: Funds: Role: Immunoglobulin Isotype Levels in Steller Sea Lions. Alaska SeaLife Center $10,000 (ended September 2002) Co-Investigator Title: Preliminary study to determine the effectiveness of intramuscular, oral, and respiratory immunizations in producing immunogen specific antibodies in the Atlantic bottlenose dolphin (Tursiops truncatus) Anheuser-Busch Sea World Parks $30,000 (ended August 2005) Co-Investigator Agency: Funds: Role: