STALLION ANATOMY AND PHYSIOLOGY 6/21/2016 1 REPRODUCTIVE TRACT OF THE STALLION CONSISTS OF BRAIN CIRCULATORY SYSTEM ENDOCRINE SYSTEM TESTICLES EPIDIDYMIS VAS DEFERENS ACCESSORY SEX GLANDS URETHRA PENIS ASSOCIATED STRUCTURES 6/21/2016 2 6/21/2016 3 TESTICLES 6/21/2016 OF ABDOMINAL ORIGIN LOCATED EXTERNALLY IN THE SCROTUM WITHIN A REFLECTION OF THE PERITONEUM KNOWN AS THE TUNICA VAGINALIS CONTAIN GAMETE CELLS AND SPERM STEM CELLS THAT PRODUCE SPERMATOZOA TUBULAR SYSTEM OF THE TESTICLE CONNECTS TO THE… 4 EPIDIDYMIS BEGINS AT THE CAUDAL END OF THE TESTICLE RUNS THE FULL LENGTH OF THE TESTICLE TO THE VAS DEFERENS 6/21/2016 5 C:\Documents and Settings\HP_Owner\Desktop\101MSDCF\D SC00084.JPG 6/21/2016 6 VAS DEFERENS TRAVERSES BACK THROUGH THE ABDOMEN TO THE CRANIAL PELVIC CAVITY CONNECTS TO THE PELVIC PORTION OF THE URETHRA 6/21/2016 7 6/21/2016 8 URETHRA PELVIC PORTION URETHRA HAS: THESE GLANDS PRODUCE THE NONSPERM PORTION OF THE EJACULATE= SECONDARY SEX GLANDS ON EACH SIDE PROSTATE SEMINAL VESICLES BULBAR URETHRAL GLAND PREJACULATE FLUID SEMINAL PLASMA GEL URETHRA EXTENDS DOWN BETWEEN HIND LEGS 6/21/2016 TO THE PENIS LOCATED ON CAUDAL VENTRAL ABDOMINAL MIDLINE JUST AHEAD OF SCROTUM PENIS CONTAINS ERECTILE TISSUE NECESSARY FOR COITUS 9 6/21/2016 10 Stallion Physiology Onset of sexual maturity 10-24 months Sperm output Semen volume/ejaculate, ml 30- 200+cc total output Sperm Rich portion usually constant around 45-70cc Gel portion changes considerable with teasing and amount of activity Sperm output 6-9 billion (fpm) Survival time with fertilizing capacity -1-2 days 6/21/2016 11 Testis Average size – 9x6x5 cm Total scrotal width > 80 mm Testicular Parenchyma-90% Seminiferous tubules (70%) Sertoli cells (30%) Increases during the long days Increase with age (2-3, 4-5) and season Leydid cells (2-12-18%) 6/21/2016 Increase with age (2-3, 4-5, 13-20) and season 12 Stallion Physiology Up to 70,000 spermatozoa/second 57 days for each cell Daily production = 3 billion or more 6/21/2016 Age and season dependent 13 Average Testicular Volume for Horse for Different Ages 6/21/2016 Age (yr) Total Testicular Volume (ml) 1.0 50 1.5 71 2.0 95 2.5 119 3.0 167 4-5 272 6-12 313 13-20 306 14 STALLIONS HAVE ANNUAL RHYTHM MUCH LIKE MARES ASSOCIATED WITH LENGTH OF DAYLIGHT/DARKNESS TESTOSTERONE PEAKS @ MIDDLE OF BREEDING SEASON TESTOSTERONE DEPRESSED IN WINTER 6/21/2016 15 SPERM HAS 3 JOBS TO ACCOMPLISH FOR SUCCESS ONCE PLACED IN BODY OF UTERUS BY EJACULATION: 6/21/2016 MOTILITY CAPACITATION EXCHANGE OF CHROMATIN MATERIAL THERE ARE METHODS TO TEST MOTILITY, ACROSOMAL FUNCTION, AND CHROMATIN QUALITY 16 MOTILITY Function of the Middle Piece Mitochrondrial Activity Consumes Energy Must be forward Progressive to be of Value 6/21/2016 17 Capasitation Function of the Acrosome Releases enzymes that react with the Zona Pellucida Takes the enzyme of many sperm to cause reaction Sperm are viable for 24hr. After 6/21/2016 18 Chromatin Transfer Function of the Head Contains Genetic material imperfict Chromatin will result in early embryo loss 6/21/2016 19 Semen Has Three Parts Pre-ejaculate Sperm Rich Sperm and seminal plasma Gel Mucous substance at end of ejaculation 6/21/2016 Clear lubricant 20 Semen output 30- 200+cc total output Sperm Rich portion usually constant around 45-70cc Gel portion changes considerable with teasing and amount of activity Sperm output 6-9 billion (fpm) Total output change with activity Maximum Standard use every 8 hr. 6/21/2016 21 Number of Sperm Depends On: Seasonal Influences (Photoperiod) Effected Areas 6/21/2016 Ejaculate volume Sperm numbers Total sperm/ejaculate Sperm motility Willingness to breed Mounts before breeding Scrotal size Testosterone production 22 Sperm Dose Under Debate Changes with Breeding Technique Industry Standard at present is considered 500 million (fpm) Higher for frozen semen Lower for specialized 6/21/2016 23 Semen Extending and Storage Use To increase Number of doses per ejaculate Used to increase sperm survival in Fresh Cooled or Frozen storage 6/21/2016 24 Centrifugation To Increase Concentration of Sperm Remove Bacteria Remove Seminal Plasma Increase Quality in storage 6/21/2016 25