PI Name: Protocol #: APPENDIX F. ADJUVANT/ANTIGEN USE 1. Indicate what antigen will be used. 2. Indicate what vehicle/adjuvant will be used: 3. Initial immunization: 4. Subsequent immunizations: 5. Anticipated complications/side effects: 6. Indicate sites for immunization: 7. Indicate route of administration: 8. What is the total and per site injection volume? 9. What is the frequency/duration of immunization (e.g., 1 injection every 2 weeks for 3 injections)? Ascites production. Fluid accumulation associated with ascites/hybridomas should not become greater than 10% of body weight. Animals must be euthanized if they become moribund or severely dyspneic. 1. Indicate the maximum volume of ascites fluid to be collected per sampling (ml/mouse) and the method of collection (i.e. skin prep, gauge needed, gravity vs. suction). 2. Indicate the number of fluid collections and anticipated frequency of collection. 3. Describe procedures used to care for and monitor the health of animals with ascites and the point of euthanasia. DOR.ORPCC.A.0F - IACUC NP Appx F Adjuvant Antigen Form updated January 2014 1 of 1