Mei Zhang
210 Copeley Rd, Apt#5
Charlottesville, VA 22903
USA
919-968-1938 (H)
434-982-4473 (W)
Email: mei.zhang@virginia.edu
Summary
Research Fellow with eighteen years of experience in molecular biology research with expertise in
mouse genetic models, embryonic stem (ES) cell, targeted mutation technology and mammalian gene
regulation. Able to rapidly master new techniques. Possessing an extremely high work ethic, and
excellent organizational skills.
Professional Experience
1981-1989
Pediatrician, Department of Internal Medicine, Nanjing Children's Hospital, Nanjing,
P.R. China
1989
Pediatrician, Microcirculation Laboratory, Department of Internal Medicine, Nanjing
Children's Hospital, Nanjing, P.R. China
1992-1995
Visiting Scholar and Research Assistant I, Division of Cardiology, Department of
Internal Medicine, University of Texas Medical School, Houston, TX
1995-1998
Research Assistant II, Division of Infectious Diseases, Department of Internal Medicine,
University of Texas Southwestern Medical Center, Dallas, TX
1998-2000
Research Associate, Division of Infectious Diseases, Department of Internal Medicine,
UT Southwestern Medical Center, Dallas, TX
2000-2002
Senior Research Associate, Departments of Pathology, UT Southwestern Medical Center,
Dallas, TX Research Analyst II, Department of Medicine, Duke University Medical
Center, Durham, NC
2002-2006
Research Analyst II, Department of Medicine, Duke University Medical Center
2007-2008
Research Fellow B, GMS, Duke-NUS Graduate Medical School, Singapore
2009- present Lab Manager, Department of Medicine, University of Virginia, Charlottesville, VA
Technical Skills:
• Embryonic stem cell and primary muscle cell cultures
• Homologous recombination techniques in mouse ES cells
• Animal care, genotyping and husbandry
• Southern blot, Northern blot, RNase protection assay, Western blot and ELISA
• PCR and RT-PCR
• Recombinant DNA technique and DNA sequencing;
• Isolation of DNA, RNA, and poly(A+) RNA from variety of tissues and cell lines
• DNA transfection and transgene gene expression analysis
• Recombinant protein purification
• Site-directed mutagenesis
• Indirect immunofluorescence
•
•
Radioisotope enzymatic AOAH assay
Genomic library screening
Publication:
1. Staab, J. F., Fosmire, S., Zhang, M., Varley, A.W., and Munford, R. S. Distinctive structural
features are shared by human, lapine, and murine acyloxyacyl hydrolases. J. Endotoxin Res. 1999, 5,
205-208.
2. M. Lu, M. Zhang, R. L. Kitchens, S. Fosmire, A. Takashima, and R. S. Munford. Stimulusdependent deacylation of bacterial lipopolysaccharide by dendritic cells. J Exp Med. 2003, 197(12):
1745-54.
3. Yan, Z., S. Choi, X. Liu, M. Zhang, J. Schageman, S. Y. Lee, R. Hart, L. Lin, F. A. Thurmond, and
R. S. Williams. Highly coordinated gene regulation in mouse skeletal muscle regeneration. J. Biol.
Chem., 2003, 278(10):8826-36.
4. Feulner JA, Lu M, Shelton JM, Zhang M, Richardson JA, Munford RS. Identification of
acyloxyacyl hydrolase, a lipopolysaccharide-detoxifying enzyme, in the murine urinary tract. Infect
Immun. 2004, 72(6):3171-8.
5. Akimoto T, Pohnert SC, Li P, Zhang M, Gumbs C, Rosenberg PB, Williams RS, Yan Z. Exercise
stimulates PGC-1α transcription in skeletal muscle through activation of the p38 MAPK pathway. J.
Biol. Chem. 2005; 280(20):19587-93.
6. Lu M, Zhang M, Takashima A, Weiss J, Apicella MA, Li XH, Yuan D, Munford RS.
Lipopolysaccharide deacylation by an endogenous lipase controls innate antibody responses to
Gram-negative bacteria. Nat. Immunol. 2005, 6(10):989-94.
7. Choi S, Liu X, Li P, Akimoto T, Lee SY, Gumbs C, Zhang M, Yan Z. Transcriptional profiling in
mouse skeletal muscle following a single bout of voluntary running: evidence of increased cell
proliferation. J. Appl. Physiol. 99(6):2406-15, 2005.
8. Li P, Akimoto T, Zhang M, Williams RS, Yan Z. Resident stem cells are not required for exerciseinduced fiber type-switching and angiogenesis, but required for activity-dependent muscle growth.
Am. J. Physiol. 2006; 290(6):C1461-8.
9. Waldrop S. Chan CC, Cagatay T, Zhang S, Rousset R, Mack J, Zeng W, Zhang M, Amanai M,
Wharton KA, Jr. An unconventional nuclear localization motif is crucial for function of the
Drosophila Wnt/wingless antagonist Naked cuticle. Genetics. 2006 Sep; 174(1):331-48.
10. Li P, Waters RE, Redfern SI, Zhang M, Mao L, Annex BH, Yan Z. Oxidative phenotype protects
myofibers from pathological insults induced by chronic heart failure in mice. Am. J. Pathol. 2007;
170(2):599-608
11. Shu Zhang, Toiga Cagatay, Mananai Amanai, Mei Zhang, Janine Kline, Diego H. Castrillon,
Raheela Ashfag, Orhan K.Oz, and Keith A. Wharton, Jr. Viable mice with compound mutations in
the Wnt/Dvl pathway antagonists nkd1 and nkd2. Mol Cell Biol. 2007 Jun;27(12):4454-64.
12. Ying Li, Ping Li, Jyeyi Kwek, Mei Zhang, Sidney Yu, Pierce Chow, Lai Chun, Jin Yi, Zeng Lin,
Takayaki Akimoto, Guangshu Hou, Ignasius Aditya Jappar, Markus R. Wenk, and Zhen Yan.
Mitophagy mediates mitochondrial degeneration and steatosis in diet-induced insulin resistance (in
submission)
13. Yu Z, Li P, Zhang M, Hannink M, Stamler JS, Yan Z. Fiber type-specific nitric oxide protects
oxidative myofibers against cachectic stimuli. PLoS ONE. 2008 May 7;3(5):e2086.
PMID: 18461174 [PubMed - indexed for MEDLINE]