Palladin and Regulation of Actin Crosslinking and Dynamics

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Palladin and Regulation of Actin Crosslinking and Dynamics
Moriah Beck, Richard Dixon, Silvia Goicoechea, Grant Murphy, Carol Otey, Sharon Campbell
Palladin is a recently discovered actin-associated protein that appears to be involved in both normal cell
migration and invasive cell motility. Recently, we demonstrated that palladin is able to both bind and
bundle actin filaments directly and revealed that one of the immunoglobulin-like domains of palladin
(Ig3) is a novel actin-binding domain. These results indicate that palladin occupies an unusual functional
niche, as it possesses the properties of both an actin-crosslinking protein and a molecular scaffold that
binds to multiple other actin-regulating proteins. In the work presented here, we determined the NMR
solution structure of palladin’s Ig3 domain and identified two basic patches that are critical for actinbinding and bundling. The results we have obtained provide insight into the manner in which palladin
binds and crosslinks actin filaments and suggest the importance of electrostatics in this interaction.
Electrostatics are also a major determinant in actin polymerization, therefore we further present
preliminary kinetic data that indicates palladin may also be capable of inducing actin polymerization. In
addition, we examined whether the Ig3 domain of palladin is required for actin organization in a cellular
context. In cells transfected with a full-length palladin construct containing a deletion of the actinbinding domain we observe dramatically altered cellular distribution of both palladin and actin, which
suggests that this interaction is critical. These findings are critical for understanding the mechanism that
palladin utilizes in promoting cell motility by identifying residues within the palladin Ig3 domain that are
important for actin binding and bundling, but also points to a mechanism for actin filament crosslinking.
Source of research support: NIH grants F32 CA139926-01 (MRB), 5RO1GM081505 and NSF MCB1121365 (CAO) and 5RO1GM080568-04 (SLC) and COBRE (8P20GM103420), KINBRE (P20RRO16475),
Flossie West Memorial Foundation Trust (MRB).
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