HOP PESTS AND THEIR CONTROL HOP PESTS AND T:PiEIR CONTROL 19)42 by H. E. :orrison Table of Contents Page Summary 1 Introduction 2 Economic Status of Fops 4 Status of Fop Project 5 19/42 Objectives 9 Seasonal History of the Hop Aphis Seasonal History of the Hop Red Spider Small Plot Testing of Effect of Timing for Fop Red Spider Control Testing of Mechanically Diluted Proprietary DH Dusts on Commercial Scale Discussion of Results Tabulation of Data Biological Method of Evaluation of Efficacy of Dust Applications Commercial Application of Ammonium Dinitroo-cresylate Dust T Discussion of Results Tabulation of Data Plant Hormone Sprays on Fops Timing of application Concentration of Material :letting of Hops Application of Material Harvesting of Crop Discussion of Results Tabulation of Data Continued Studies of Red Spider Control on Strawberries Discussion of Results Tabulation of Data Phytocidal Studies of DN Dusts Completion of Chemical Studies of DN Dusted Hops Sw-r-estions For Future Tiork 10 10 11 12 15 13 25 27 29 30 29 31 31 31 32 32 33 35 54 36 38 40 112 43 RU 25 14.04) rEsts+ Their 63A-1-m1 Spiat; HOP RED SPIDER (Tetranychus) Sp. HOP PESTS AND THEIR CONTROL 1942 Report to the Department of Entomology Oregon State College Summary The general progress of the hop pest project is reviewed with a discussion of the economic status of hops and the hop pest project. A plan is submitted whereby some of the losses caused by hop pests might be eliminated. Seasonal history studies and control work were restricted because of the war emergency. Testing of the effect of timing of dust applications were abandoned because of the severe inroads of downy mildew and the resulting poor stand of hops in the experimental yards. Commercial application of mechanically diluted proprietary DN Dusts resulted in reduced controls and reduced efficiency of application. Commercial application of the ammonium dinitro cresylate dust gave inferior results than the proprietary DN dusts. This is believed to be due to mechanical mixtures of the former dust in contrast to the atomized dusts of the latter. Preliminary testing of Fruitone (a plant hormone) for stimulation of hops and prevention of cone shattering resulted in inconclusive results. Difficulty in wetting of hops and possible chemical action between wetting agents employed with the fruitone may have resulted unfavorably. DN dusts were applied to strawberries for the second successive year without foliage injury and with satisfactory control of red spiders. Work was continued in the testing of DN dusts on various crops. Nine new species were dusted with DN Dust D-4 and only slight foliage injury was noted on two of these varieties. 2 A method of evaluation of efficiency of rating mechanical applications of dusts on hops by biological measurement has been devised. The chemical analysis of hops dusted with DN Dust and DN Dust D-4 which was started in 1940 was completed. No difference between dusted and undusted hops was noted. Hop Pests and Their Control - 1942 Introduction The results of the 1941 season demonstrated the improvement of the Trials were conducted with three proprietary dusts experimental duster. (DN Dust, DN Dust D-4 and DN Dust D-8) at rates ranging in amounts from 10 to 60 pounds to the acre. It was noted that 40 pounds to the acre was satisfactory for commercial control and this resulted in a savings of 10 pounds to the acre over the 1940 tests. The monetary value of this savings was estimated as $1.00 per acre. Seasonal development of the hop aphis and hop red spiders was studied and data were set aside for special reports. Severe injury to hops resulted in various localities in the Willamette Valley as a result of the hop red spider. The peak of infestation occurred very late in the season and in general most hop growers were able to harvest the crop before spider damage became acute. The hop aphis invaded the yards early and serious damage resulted early in the season. Several very hot days during the latter part of July killed the majority of the aphis. The population never recovered from this natural control until very late in the season and after hops had been harvested. Several materials were dusted on hop plants at this time in an effort to test sooty mold control in the field. were not promising. These studies 3 Small plot testing of new and untried toxicants were carried on in the experimental hopyard. It was found that the mechanical mixture of proprietary nicotine sulfate dusts and DN dusts resulted in lowering of the toxicity of the DN dusts to red spiders. Mechanical dilutions of DN dusts with proprietary rotenone dusts did not show this incompatibility but hop aphis were not controlled. Population build up studied were carried on at various intervals until fifty (SO) days after dusting. It was learned that the proprietary dinitro cresol dusts gave a very high initial control but that populations build up very rapidly after dusting. than the undusted plots. The rate of increase was more rapid The several DN dusts and the DN-rotenone dust mixtures showed a marked contrast and populations were held in check for a period of fifty days after dusting. The guanadine and ammonium salt of dinitro-o-cresol were inferior to the proprietary DN dusts but superior to the proprietary dinitro-o-cresol dusts. Some trouble was noted in attempting to atomize these toxicants into the carrier and it was thought that better results might be obtained with better mixing. Attempts were made to check growers' applications of DN dusts. It was found in general that little attempts were made to control the hop red spider and that growers had not equipped themselves with efficient machinery. Proprietary DN dusts were applied to 10 acres of strawberries for red spider control. Preliminary studies showed that with satisfactory power equipment, the red spiders could be contacted on the undersurfaces of the leaves. Strawberries are very susceptible to DN dusts and it was learned that the critical temperature for strawberry dusting was 70 degrees F. Satisfactory controls were effected with DN Dust D-4 and DN dust D-7 at 100 pounds to the acre. DN Dust D-8 proved too heavy for the fan in the small experimental unit and dusts became clogged in the air stream. DN Dust D-4 was applied to a small planting of pansies with no foliage injury and excellent control of red spiders. Attempts were made to control the California prionid in hop poles and hop crowns with the use of dichlorethyl ether, chloropicrin and methylisobutyl ketone. These attempts were unsuccessful. The Economic Status of Hops Hops are the most important of Oregon's specialty crops. The five- year average (1936-1940) of cash farm income derived from hop production has been computed as $3,625,000.00. Foreign hops which normally entered into competition with domestic hops were greatly curtailed during 1941 as a result of World War II. This resulted in greatly increased prices and a raise in cash farm income to *4,040,000.00. The severe inroads of downy mildew on hops in the spring of 1942 greatly decreased production in Oregon. This factor combined with the total elimination of foreign hops from the market (United States entry into the war) has resulted in hops reaching an unprecedented high. and $1.50 (seedless) at this writing. Hops are quoted at $1.30 (seeded) Many growers had already con- tracted their hops for 1942 at $.30 and $.35 and it is not possible to predict the cash farm income at the present time. ever, that the figure may exceed $5,000,000.00. taken for $.70 and higher hops for 1943. It is possible, how- Many contracts have been If a normal crop is assured for 1943, the cash farm income may exceed $10,000,000.00. The role of hops as an essential crop to the war effort has not been definitely established. Considerable beer is reported to have been 5 consumed by the allied armies who are stationed in tropical climates where water is often scarce and impure. The prospect of an exceedingly high farm income makes hop production very important to Oregon agriculture. There is some possibility that hop vines may serve for manufacture of fiber and this would contribute directly to the war effort. The large capital investment required in hop production makes it unlikely that hop growing will be greatly curtailed during the near future. camouflage. There is also some interest in the growing of hops for The vines grow rapidly and may serve this purpose readily. Status of the Hop Project The importance of hop pest control depends upon the importance placed on the hop industry for the duration of the war. carried on in hop pest control since 1937. hop red spider has been developed. Work has been Economic control for the This phase of pest control has neces- sitated the development of efficient dusting machinery. This was accom- plished through the cooperative efforts of the Departments of Agricultural Engineering and Entomology and with the United States Department of Agriculture, Division of Drug and Related Crops. Demonstration dustings were carried on in seven different localities during 1940. any growers were present at these demonstrations as well as demonstrations at the hop field days of 1940 and 1941. The problem of motivation of hop growers is difficult. Since 1940 there are a total of six efficient dusters in Oregon which are capable of effecting satisfactory applications. 3,300 acres of hops in Oregon. for this acreage. This averages one duster for every There are at least 96 more units needed One grower who cooperated with the Department of Entomology during the 1940 investigations and who had equipped himself 6 with a very efficient unit, has been reported to have dismantled his equipment and again was dusting according to old methods. He has not been successful in effecting economic control during 1942 and has reported that the recommended dusts were unsatisfactory. Hop growers from Washington State and from British Columbia have taken to the Oregon recommendations more readily than have Oregon hop growers. This is evidenced by the following statistics. Number of efficient dusters Acreage of hops State British Columbia Washington Oregon 1,500 7,600 20,000 Acres dusted 1941 State British Columbia Washington Oregon 2 8 6 Remarks 1 duster to 700 acres 1 duster to 950 acres 1 duster to 3,300 acres Acres 7767E7ITIEusts dusted Percent of Percent of Acres total acres dusted total acres dusted 19)41 dusted 1942 1942 100 400 100 6.9 5.9 0.5 120 1800 2250 8.3 23.7 11.3 Note--No figures available from California. Correspondence with Dr. Sid Braucher of the Dow Chemical has shown that the following large growers from British Columbia and Washington have adopted the red spider control program. Golding Farms (John I. Haas), Yakima, Washington Yakima Chief Ranches (Lloyd Hughes), Yakima, Wash. Victor BelairelMoxie City, Washington W. C. Rivard, Maxie City, Washington Charvet Brothers, Grandview, Washington Horst Company, Agassiz British Columbia H. M. Ord, Chilliwack, British Columbia Additional information was obtained from L. G. Smith, Extension Entomologist of Washington State. He writes. . "Growers reported excellent results, 1941 Hop Field iiay EXPERILENTAL HOP DUSTR 7 namely Lloyd Hughes Inc. and John I. Haas. Most of the dust was applied with effective power equipment at the rate of 60 lbs. per acre. Richey & Gilbert... the sole dealers in Yakima County, sold approximately )43 tons." The failure of Oregon hop growers to respond is believed to be due largely to lack in extension work. Comparatively few Oregon hop growers are known to consult with county agents and extension officials. Essen tial information apparently has not been made available to them. Two factors are largely responsible for lack of hop pest control. 1. Inefficient equipment. They are: This could have been remedied prior to 1943 with comparatively little cost. Most growers are equipped with machinery which could be adapted to meet approved specifications. New duster fans, flexible metal tubing are scarce or not available. The approved dust will not effect control with the old type machinery. Further research of the Departments of Agricultural Engineering and Entomology may solve this problem if such a program is thought advisable. 2. Improper timing of dust applications. Most hop growers are faniliar with red spider injury but few of them have ever seen the pest. This is due largely to protective coloration and smallness of the red spider. Most growers are not aware of the abundance of the pest until foliage has assumed either a mottled green or rusty brown color. This usually signifies that hop plants are very heavily infested and that red spiders will soon migrate into hop cones. Control measures applied at this time are often too late to prevent crop injury. Some thought has been given this problem and several methods suggest themselves for their solution. Hop growers of the Pacific Coast States 8 are now well organized for the marketing of hops. This has been accom- plished by adequate leadership and. by cooperation with the United estates Department of Agriculture, Marketing Administration, Fruit and Vegetable One-fourth cent ($0.0025) is assessed to growers for each pound branch. of dry hops handled. Funds raised in this manner are used to defray all expenses. It is believed that this organization could also serve to coordinate and finance a cooperative pest control program. The 20,000 acres of Oregon hops should be divided into various districts and from six to eight men employed for scouting of yards and direction of pest control operations. The present scarcity of agricultural labor suggests the training of youths (ages of 15 to 17 years) by the Departments of Agricultural Engineering, Farms Crops and Entomology in cooperation with the United States Department of Agriculture, Division of Drug and Related Plants for carrying on of this work. It is possible that a program of this kind may work more readily if handled through and in cooperation with the newly organized Voluntary Community Leadership Organization which is fully described in Extension Bulletin S99. Some of the responsibilities assumed by these individuals after having been trained may be listed as follows: 1. Regular scouting of hop acreage in their respective district for the presence of hop aphis, hop red spider, downy mildew and other pests throughout the season. 2. Direction of pest control operations in infested areas if and when needed. 9 3. Direction of servicing of dusters, sprayers, and other equipment during the winter months in order that this equipment should be in readiness for pest control operations during the critical growing season. It. Pooling of resources and forming cooperative buying agency for purchase of insecticides, fungicides, and other items necessary for hop production and pest control. Oregon hop growers spend an average of 000,000.00 annually for pest control. Inefficient control and non-control often cost these growers an additional $750,000.00. The expenditure of $20,000.00 annually by hop growers for cooperative pest control and pest prevention could be raised by assessing growers one mill ($0.001) for each pound of dried hops. It is felt that an investment of this amount would eliminate non-control costs and also aid in reduction of material cost of pest control. The program would also assist in increased production of hops, lower the per acre cost of production, and increase the quality of the merchandise. 19112 Objectives The outbreak of World War II made it necessary to greatly curtail hop pest investigations. unavailable. 1. Travel was greatly restricted and labor was The following objectives were accordingly planned. Restricted seasonal history studies of the hop aphis and the hop red spider. 2. Small plot tests to study the effect of timing on red spider control. 3. Testing of mechanically diluted proprietary DN dusts on a commercial scale. 1914 Eor FIELD DAY 10 4. Commercial application of ammonium dinitro cresylate dust. 5. Instituting of preliminary trials of the effects of the hormone spray (Fruitone) on hops. 6. Continued work on red spider control on strawberries. 7. Phytocidal studies of DN dusts. 8. Completion of chemical studies of DN dusted hops. Procedure Seasonal history studies - The hop aphis Restricted labor and travel facilities made it necessary to limit or restrict qualitative and quantitative collection of data. All observa- tions were made in the College Experimental Hopyard, East Farm, Corvallis, It will be recalled from the 1941 report that a large number of Oregon. hop aphis eggs were deposited on plum trees adjacent to the Experimental hopyard, These eggs were watched throughout the fall and winter months. A severe "silver thaw" occurred early in January 1942 and all trees in the vicinity of Corvallis were encrusted with a heavy coating of ice. A number of checks were made after this occurrence and no hop aphis eggs could be found. Continued checks during the spring months showed that hop aphis were no longer present on these trees. It is possible that this natural factor contributed to the extremely light infestation of aphis in hopyards during 1942. Temperatures exceeded 100 degrees F. during the early part of July and this resulted in added natural control. Aphis populations did not reach a peak until the latter part of November and hops were harvested before economic damage was effected. Seasonal history of the hop red spider The spring of 1942 was very wet and cool. Red spiders were found on hop foliage during the latter part of May but development was probably 11 retarded by climatic conditions. Warm dry winds were prevalent during the latter part of June and vegetation about the hopyard became desiccated. Red spiders immediately began to increase rapidly after this dry period. In all probability the increase could be credited to migration from desiccated plants to hops. Red spiders reached their peak of abundance during the latter part of August and the beginning of September. Con- siderable damage to hop cones was noted in several trips through the Willamette Valley during harvest time. Few growers attempted control measures and little attempts were made to purchase or obtain efficient dusting equipment. There were an estimated six dusters which were capable of doing a satisfactory job in the 20,000 acres of hops. At least 96 more dusters are estimated as necessary for serving this acreage. Red spider control as recommended by the Oregon Entomology Department was adopted on a large scale by Washington hop growers. Approximately L3 tons of DN dusts were sold for red spider control in the Yakima Valley during 1942. Small plot tests to study the effect of timing on hop red spider control The phenomenol action of the proprietary DN dusts in preventing red spider development for as long as 50 days after dusting led to the possibility of applying these dusts to hops early in the season before plants had reached maturity and probably preventing red spiders from establishing themselves. If this were possible, considerable savings may be effected by applying less dust per acre. With this in mind, a series of 16 plots of eight replicates each were laid out in the Entomology section of the experimental hopyard. DN Dust D-4 to these plots according It was planned to apply to the following schedule. 12 Plot No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Date of application No application June 1 June 8 June 15 June 22 June 29 July 6 July 13 July 20 July 27 August 3 August 10 August 17 August 24 August 31 June 1, 8, 15, 22, 29, July 6, 13, 20, 27 August 3, 10, 17, 24, and 31 It was planned to make intensive studies on the rate of population buildup upon all of these plots at weekly intervals throughout the season. Information of this kind it was thought would be of great value in making control recommendations. It was unfortunate that circumstances prevented the carrying out of this program. The experimental yard was severely damaged by downy mildew which either killed or severely stunted the majority of the hop plants. It was not until the middle of July that any plants assumed signs of normal growth. This was too late to undertake the above experiment. The thought is left at this time as one piece of unfinished business which should be attempted at some future time. Testing of mechanically diluted proprietary DN dusts on a commercial scale Preliminary experiments were undertaken with DN dusts in 1937. The proprietary DN dust and mechanical dilutions of the proprietary dust to concentrations of 0.75, 0.50, 0.25, 0.125, 0.0625, and 0.03125%. Walnut shell flour was used as the diluent in all instances. It will be 13 recalled that these dusts were applied with a small power duster in the same manner as spray applications. Attention was given to coverage of undersurfaces of hop leaves and no effort was made to control amounts per acre (See 1939 report to Department of Entomology). It was thought advisable to attempt to duplicate this experiment when dusts were applied with a commercial hop duster. It was thought that if mechanical dilutions were proven to giver, adequate control, considerable savings could be made to the grower. For example: DN Dust D-4 at 40 lbs. per acre costs $4.00 DN Dust D-4 mechanically diluted to I% in frianite and applied at 60 lbs. per acre 83.00 DN Dust D-4 mechanically diluted to *% and applied at 80 lbs. per acre $2.80 Two proprietary dusts (DN Dust D-4 and DN Dust D-3) were mechanically diluted in frianite at the ratio of 50-50 and 25-75. are tabulated as follows. scored August 22, 1942. These treatments Dusts were applied August 18 and results were Fifty leaves were scored according to number of living and dead red spiders in accordance with techniques reported in previous reports. i)t Treatment Treatment No. 1. 2. 3. 5. 6. Amt. toxicant per acre DN Dust D-14 DCHA salt of DNOCHP DNOCHP Frianite 1% 1 % 98i% 40 lbs. 0.60 lbs. DN Dust D-8 DCHA salt of DNOCHP DNOCHP Oil (Heavy) Frianite 1% 40 lbs. 0.60 lbs. 1 % 2 % 961% 60 lbs. 0.45 lbs. 60 lbs. 0.45 lbs. 80 lbs. 0.30 lbs. 80 lbs. 0.30 lbs. DN Dust D-4 (Mechanically diluted with frianite 50-50) DCHA salt of T)NOCHP DNOCHP Frianite 4. Amt. per acre DN Dust D-8 (Mechanically diluted with frianite 50-50) DCHA salt of DNOCHP DNOCHP Oil (Heavy) Frianite DN Dust D-4 (Mechanically diluted with frianite 25-75) DCHA salt of DNOCHP DNOCHP Frianite DN Dust D-8 (Mechanically diluted with frianite 25-75) DCHA salt of DNOCHP DNOCHP Oil (Heavy) Frianite 1% % 99-41 leg c,/ -n-, 1 % 98% 1/ 995,8% 1/8% 14 n 2/Q 991/8% The results of these tests departed widely from the hypothetical assumption. DN Dust DN Dust DN Dust DN Dust DN Dust DN Dust Check - The following effectiveness is tabulated as follows. D-4 D-8 D-4--Frianite (50-50) D-8--Frianite (50-50) D-4 -- Frianite (25-75) D-8 -- Frianite (25-75) No treatment % Kill Standard deviation 98.1 98.7 90.8 88.1 79.8 82.8 6.5 2.655 3.359 12.118 12.657 15.330 14.544 6.643 S.E.M. 0.377 0.475 1.714 1.789 2.168 2.057 0.939 15 Discussion of results The results of these tests were very interesting. It was evident that: 1. Mechanical dilution of the proprietary dusts resulted in greater lowering in effectiveness. The amount of toxicant applied according to treatment is listed in the following table. The amounts of toxicant required for satisfactory kills based on laboratory studies and computed on an acreage basis according to the report to the department of Entomology in 1940 (page 57). Treat No. 1 2 3 4 5 6 Treatment DN Dust D-4 DN Dust D-8 DN DN DN DN Dust Dust Dust Dust D-4 D-8 D-4 D-8 --Frianite - -Frianite - -Frianite - -Frianite (50-50) (50-50) (25-75) (25-75) Lbs. toxicants per acre Computed toxicant needed for control 0.60 0.60 0.45 0.45 0.30 0.30 0.05 0.05 0.05 0.05 0.05 0.05 It will be noted that at the lowest amount of toxicant applied the dosage was six times higher than that of the computed amounts necessary for satisfactory control. 2. One factor which was noted in the scoring of the effectiveness of the treatments is not indicated by the data. In nearly all leaves examined, coverage of foliage was considered adequate. In many instances, sporadic kills were noted on the dusted foliage. A portion of a leaf was often observed to have one hundred percent kill on 1/2 to 3/4 of its surface and 0 percent kill on the remainder. This suggested that mixing of the diluted dusts might offer the explanation. Samples of the dusts were 16 accordingly submitted to Dr. R. H. Robinson for analysis. His findings are reported as follows: Dust % Dicyclo salt of DN 1.01 DN - D-4 50-50 mix of #1 25-75 mix of #1 DN - D-8 50-50 mix of #4 25-75 mix of #t Ammon. dinitro cresol 1. 2. 3. I. 5. 6. 7. .53 .27 1.02 .49 .27 .97 These analyses show clearly that the mixing of the diluted dusts were very satisfactory. Several hypothesis may be accordingly advanced to explain the sporadic kills. 1. The proprietary dusts are mixed by atomizing the toxicant unto the carrier. Each dust particle is therefore supposedly coated with the toxic material. Mechanical diluting of this dust to 1 and i percent of the original must in effect reduce the number of toxic particles by 50 and 75%. Increase of rates of application from 40 to 60 and 80 lbs. per acre respectively would serve to cut the number of toxic particles to .75 and 50 percent of the original. The reduction in toxicity separates itself according to these dilutions but does not explain the sporadic type of kill. This type of kill was not noted in the plots treated with the proprietary dusts. A difference in specific gravity between the toxic and non-toxic particles suggests that these may settle out in the airstream leaving only part of the foliage covered with toxic particles. Laboratory studies should be undertaken in order to test this hypothesis. Walnut shell flour was used as the diluent in the 1939 investigations in which satisfactory kills were obtained by mechanically diluting 17 DN dust. It will be recalled that satisfactory kills were obtained in commercial applications of DN dust D-3 (i% toxicant in walnut shell flour) at 50 lbs./acre. Decidedly inferior results with DN Dust D-7 (i% dicyclo- hexylamine salt of DN dust in frianite) at rates of 100 lbs./acre. All studies on hops to date show that there is no difference in toxicity between the proprietary DN dust and DN dust D-4 when frianite is used as a carrier. This suggests that the difference in toxicity between the proprietary dusts and the mechanically diluted dusts may be due to the physical and/or chemical properties of the carriers employed. studies should he undertaken to explor this possibility. tests are submitted as follows: Laboratory Data from these 18 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Date of dust application - Aug. 18, 1942 Material - DN Dust D-4 Rate of application - 40 pounds per acre Equipment used - Experimental hop duster Corvallis, Oregon Data collected: Aug. 22, 1942 Plant Leaf Plant Leaf No. No. No. No. 6 3 1 Mean Number of red spiders Dead Alive Total % dead 0 I---- 45 100.0 45 2 85 0 85 100.0 3 54 3 94.7 57 49 0 100.0 4 49 63 63 0 100.0 5 299 286 99.0 3 1 2 2 216 21 237 2 422- 3 144 139 18 0 440 144 7B 1 79 913.11 5 131 0 0 8 114 143 174 14 641 96.2 100.0 100.0 95.4 97.8 8 2g.5 12 0 16 242 1 2 217 230 115 145 118 2 115 161 120 825 38 863 3 223 735 213 0 0 0 223 735 213 4 5 421 112 3 424 .0 112 1707 3 4 5 Mean 1 2 Mean 1 92.1 96.6 94.6 100.0 97.3 126 114 143 166 627 Mean 1767----3 Liean 100.0 2 3 4 5 67 38 148 92 136 481 1 4 5 5 3 1468 Mean 4 0 5 5 0 13 3 4 5 3 67 35 143 87 136 2 Number of red spiders Dead Alive Total % Dead 130 0 1)0 100.6 129 132 3 97.7 127 127 0 100.0 119 119 100.0 108 108 0 100.0 616 613 99.5 3 96.4 95.0 100.0 90.1 98.3 95.6 100.0 100.0 100.0 99.3 100.0 99.ff 7 4 5 Wan 1 2 8 3 4 5 Mean f 2 9 3 4 5 Mean 1 2 10 3 4 5 2 141 277 8 1198 49 285 1247 417 0 0 209 167 144 191 3 5 2 112ti 10 -235 0 14 172 185 341 8 292 0 0 1223 22 277 388 0 0 405 119 23 78 0 0 1267 Mean 23 Vt. Mean 9351 178 Ari . Mean Standard deviation Standard error of mean Efficiency rating 417 209 170 149 193 1138 235 186 193 341 292 1247 277 388 428 119 78 129 0 9529 91.1 95.9 100.0 98.6 97.2 96.1 106).0 100.0 98.2 96.6 98.9 99.1 100.0 92.5 95.9 100.0 100.0 98.2 1b0.0 100.0 94.6 100.0 100.0 96.2 98.1 9 .09 2.655 0.377 81% 19 Population Counts 1942 Hop Red Spider Investigations East Farm Corvallis, Oregon Data collected: Aug. 22, 1942 Oregon State College Date of dust application - Aug. 18, 1942 Material - DN Dust D-4 (Mechanically diluted with Frianite 50-50) Rate of application - 60 lbs./acre Equipment used - Experimental hop duster Plant Leaf No. 1 No. 23 2 583 84 230 53 67 78 998 2 80 136 1134 450 260 268 170 273 17 2 L67 260 268 170 275 114 19 114/10 408 217 194 320 105 63 42 19 0 0 124 471 259 4 5 Mean 1 2 2 3 4 5 Mean 1 2 3 3 4 5 Mean 1.244 1 2 3 4 5 150 Mean 1 2 5 3 4 5 Mean 377 165 107 212 389 437 291 327 503 145 1703 9 5 32 588 137 297 1 3 Plant Leaf Number of red spiders Dead Alive Total- % dead 0 0 0 213 320 105 1368 71.9 99.1 61.3 77.4 97.5 96.4 100.0 100.0 100.0 1 6 3 14 288 98.4 100.0 100.0 75.4 83 7/1 75.9 92.9 3 5 0 0 4 5 92 63 30 716 55 4 5 Mean 20 272 -----1----7-47-77-73:17-37.72 9 3 4 5 81.5 Mean Note: Sporadic kills observed on foliage. Portions of leaf had from 100 to 0% kills. Dust coverage good in nearly every instance. 313 116 137 122 308 116 137 3 2 90.3 97.o 100.0 100.0 100.0 96.8 187 1416 14]. 1 484 300 327 503 145 1759 1 12 64 87.8 96.9 99.5 99.6 93.6 95.5 296 246 364 271 175 1352 83.8 91.1 100.0 100.0 90.9 47 9 0 0 0 56 337 254 366 104 86.7 81 283 8 2 )144 98./ Mean 97.1 76.4 56.4 82.8 82.7 100.0 81.2 98.3 100.0 92.7 0 1325 2 8 480 127 85 293 444 1429 83 16 5 0 1 7 Number of red spiders Dead Alive Total % dead 397 127 69 2 99.3 100.0 164 No. 88.0 Lean 377 170 140 376 470 1533 0 5 33 No. 10 Van- 196 89 247 118 694 1 47 2 178 307 271 144 947 11650 3 4 5 156 ?87 95 260 127 850 68.3 93.7 95.0 92.9 81.6 5 0 0 52 178 307 90.4 100.0 100.0 91 6 13 9 8 12 25 1022 Wt. Mean Arith. mean Standard deviation Standard error of mean Efficiency rating 279 156 9'72 12672 97.1 92.3 97.4 91.9 90.76 12.118 1.714 58% 20 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Corvallis, Oregon Date of dust application: Aug. 18, 1942 Data collected: Aug. 22, 1942 Materials DM Dust D-4 (Mechanically diluted with Frianite 25-75) Rate of application: 80 lbs./acre Equipment used: Experimental hop duster Plant Leaf No. No. 2 66 185 0 15 3 249 4 5 66 302 87 5 1 1 Mean 568 1 3 316 178 45 4 5 73 226 2 2 Mean 838 1 2 3 3 4 5 Mean 1 2 4 3 4 5 Mean 5 103 129 267 46 97 642 316 118 225 187 302 1148 66 200 336 71 497 1170 145 12 67 19 461 190 112 9 252 235 1090 41 73 144 202 19 68 4o 241 286 114 137 583 13 96.0 36.5 383 329 323 291 278 310 1531 76.2 99.1 64.2 91.5 93.6 86.2 205 66 91 8 92 144 45 1139 2 212 63 97 88 2 214 98 106 94 701 3 604 100.0 92.5 74.1 92.9 60.7 195 302 1 4 5 Mean Number of red spiders Total % dead Dead Alive 35 9 6 97 74.2 Plant Leaf No. 6 Number of red spiders Total % dead No. Dead Alive 83 27 110 2 95 274 71 531 1054 38 52 18 20 133 326 89 3 5 Mean 68.5 1 278 93.7 40.2 79.3 96.2 76.9 2 389 3 288 4 5 479 65 1499 71.5 63.9 93.3 40.4 70.8 72.7 77.3 67.3 97.4 74.9 Note: Sporadic kills observed on Portions of leaf had foliage. from 100 to 0% kills. Dust coverage good in nearly every instance. 7 Mean 1 2 8 3 4 5 Mean 2 9 3 4 5 Wan 1 2 10 3 4 155 36 65 12 0 22 87 1634 45 75 244 875 16 87 252 112 5 69 279 66 54 473 314 127 1271 73 229 146 232 147 827 9629 314 454 300 479 135 149 302 108 297 421 551 1209 81 23 19 7 28 29 63 5 Mean 146 Wt. mean 2436 Arith. mean Standard deviation Standard error of mean Efficiency rating 75.5 71.4 84.o 79.7 96.4 57.2 88.5 85.7 96.0 100.0 74.7 91.7 230 64.8 347 87.0 131 82.4 91 82.4 331 73.7 1130 ----7777- 117 366 700 380 181 1744 92 236 174 261 210 973 12065 95.7 81.1 60.1 82.6 70.2 72.9 79.3 97.0 83.9 88.9 70.0 84.9 79.5 79.76 15.330 2.168 62% 21 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Date of dust application: Aug. 18, 1942 Material: DN Dust D-8 Rate of application: 40 lbs./acre Equipment used: Experimental hop duster Plant Leaf No. No. 1 0 5 314 100.0 1 65 227 2 247 37 14 0 56 215 299 197 1252 3 285 197 1196 97.8 82.8 95.3 100.0 95.5 315 194 39 860 433 97 84 316 0 0 0 14 204 1134 8 22 267 317 63 149 108 904 0 0 0 0 0 0 1 b9 2 42 5 3 3 315 205 397 1028 0 0 0 ei 45 315 205 397 1036 1 3111 8 322- 2 201 7 208 3 4 5 349 166 169 1199 0 0 349 3 4 1 2 3 1 2 3 4 5r Mean 4 5 Mean Mean Number of red spiders Total % Dead Dead Alive 222 178 Mean 5 No. 314 4 5 4 Leaf No. 2 5 3 Piant 1 Mean 2 NuMber of red spiders Dead Alive 'Dotal % dead Corvallis, Oregon Data collected: Aug. 22, 1942 0 15 433 97 84 330 212 1156 267 317 63 149 108 904 74 100.0 100.0 100.0 95.8 96.2 98.1 100.0 100.0 100.0 100.0 100.0 100.0 6 4 5 Mean 1 2 7 4 5 Mean 1 2 8 Mean 1 2 9 166 169 1214 98.8 3 4 5 Mean 97.5 96.6 100.0 100.0 100.0 3 4 5 9)22 93.3 100.0 100.0 100.0 99:2 3 87 167 141 45 197 637 4 0 3 0 0 199 347 214 308 16 29 3 4 5 0 100.0 100.0 98.7 100.0 100.0 99.5 91 167 144 45 197 644 95.6 100.0 97.9 100.0 100.0 98.9 215 376 214 318 102 1225 92.6 92.3 100.0 97 1165 60 217 443 191 287 411 21 0 4 9 11 45 443 195 296 422 91.2 100.0 97.9 96.9 97.4 1594 '97.2 14 0 0 5 10 29 246 157 245 316 432 195 1375 11264 1'73 10 7 65 247 319 194 39 864 10 5 1549 2 0 0 4 0 0 4 245 316 427 185 1346 Mean 11018 Wt. mean Arith. mean Standard deviation Standard error of mean Efficiency rating -238 96.9 95.1 95.1 92.5 100.0 100.0 98.8 94.9 97.9 97.8 98.74 3.359 0.475 66% 22 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Date of dust application: Aug. 18, 1942 Material: DN Dust D.-8 (Mechanically diluted with Frianite 50-50) Rate of application: 60 lbs./acre Equipment used: Experimental hop duster Plant Leaf No. 1 No. 1 95 2 32 3 39 47 39 4 5 Mean 252 1 2 2 3 4 5 Mean 3 4 5 Mean 1 4 50 33 134 67 59 3143 2 3 Number of red spiders Total- % dead Dead Alive 23 4 5 Mean 149 275 513 109 313 1359 -47 67 0 3 8 3 81 8 0 5 3 5 21 12 64 89 0 5 170 6 4 T4 9 145 378 967 13 608 311 205 505 2034 162 32 42 55 42 333 58.6 100.0 92.9 85.5 92.9 75.1 58 33 139 70 86.2 100.0 96.4 95.7 64 364 92.2 94.2 161 339 602 109 318 1529 92.5 81.1 85.2 7) 137 100.0 98.4 88.9 88.1 97.9 96.0 8 223 158 386 40 1007 91.8 97.9 96.0 0 6 5 608 317 210 584 2124 100.0 98.1 97.6 86.5 95.5 Corvallis, Oregon Data collected: Aug. 22, 1942 Plant Leaf No. No. 1 2 6 3 4 5 Mean 1 2 7 3 4 5 Mean 2 3 4 Mean 1 2 9 3 4 5 Mean 3 4 5 Mean Note: 79 90 Sporadic kills observed on foliage. Portions of leaf had from 100 to 0% kills. Dust coverage good in nearly every instance. 10 407 145 89 371 216 27 82 167 5 0 281 169 72 63 0 5 309 67 195 122 48 316 17 42 0 0 768 62 219 427 95 158 213 1112 41 2 189 473 3 87 1 5 413 263 305 89 221 1291 1228 1 8 Number of red spiders Dead Alive Total % dead 3 8 19 73 81 222 41 0 72 19 165 204 81 Mean 1115 213 10472 Wt. mean 1489 Arith. mean Standard deviation Standard error of mean Efficiency rating .. 4 5 440 345 472 94 221 1572 94.9 76.2 64.6 94.7 100.0 82.1 576 217 152 371 221 1537 /0.7 66.8 58.6 100.0 97.7 79.9 104 237 122 83.7 82.3 100.0 48 319 830 100.0 99.1 92.5 260 435 114 231 294 84.2 98.2 83.3 68.4 72.4 83.3 -1334 ZO 82.2 473 159 184 285 100.0 54.7 1)31 11961 89.7 71.5 83.9 87.6 88.12 12.657 1.789 61% 23 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Corvallis, Oregon Date of dust application: Aug. 18, 1942 Data collected: Aug. 22, 1942 Material: DN Dust D-8 (Mechanically diluted vith Frianite 25-75) Rate of application: 80 pounds per acre Equipment used: Experimental hop duster Plant Leaf No. No. 1 2 1 3 4 5 Mean 1 2 2 3 4 5 Mean 1 3 2 3 4 5 5 100.0 1 99.3 2 113 330 215 452 318 1428 84.1 71.8 92.1 82.1 84.6 81.9 180 92.2 90.2 79.5 59.1 67.6 76.1 7 33 6 78 164 468 83 44 239 28o 218 1184 98 3 116 148 1514 72 115 195 95 237 198 371 269 18 305 3 97 205 169 942 93 17 81 49 258 14 33 35 142 81 295 303 219 57 2 3 145 78 64 0 191 936 29 233 4 5 Mean 473 684 101 188 263 1709 166 1 No. 0 5 2 Mean Leaf No. 473 679 1 4 5 Plant 26 158 390 156 236 966 1170 Mean 4 Number of red spiders Dead Alive Total % dead 83 338 122 347 250 1237 360 302 209 78 220 1169 78.8 96.3 83.3 65.3 84.3 81.6 97.0 61.7 56.3 88.6 84.2 72.5 69.4 100.0 86.8 80.1 Note: Sporadic kills observed on foliage. Portions of leaf had from 100 to 0% kills. Dust coverage good in nearly every instance. 1 189 217 169 231 165 971 2 6 3 4 5 Idean 7 3 4 5 Mean 2 8 3 4 5 Mean 9 67 41 8 52 9 177 218 73 952 5 0 89 73 79.9 loo.o 98.1 97.8 100.0 1041 91.5 201 45 63 395 142 846 49 250 7 52 89 152 397 163 1014 80.4 86.5 41.4 99.5 185 407 3 2 21 168 3 89 4 5 ., 645 63 0 97 15 97 7 1423 182 133 230 42 18 5 36 41 2 3 96 42 5 352 853 10573 Mean Wt. mean 174 1145 73.8 84.1 95.5 81.6 94.8 84.6 81 0 2 -1 256 258 177 283 322 185 154 1 1[e an 10 Number of red spiders Dead Alive Total % dead 142 1937 403 185 157 223 248 407 186 660 104 1605 175 248 101 78 393 995 11530 87.1 83.4 74.6 100.0 47.8 97.7 93.3 86.7 76.6 92.7 95.0 53.8 89.6 85.7 84.4 MM. mean 8273 Standard deviation Standard error of mean Efficiency rating 14.544 2.057 62% 24 Population Counts 1942 Hop Red Spider Investigations East Farm Corvallis, Oregon Oregon State College Data collected: Aug. 22, 3942 Materials Check treatment - No dust application Plant Leaf No. No. 1 2 1 3 4 5 Mean 2 371 128 949 130 387 140 1045 95 8.4 8.7 26.9 4.1 8.6 9.2 19 23 125 136 144 159 3 0 3141 3141 4 5 8 577 422 1601 585 434 1663 328 219 169 375 381 1472 342 389 1569 4.1 11.6 8.6 5.3 4.3 6./ 147 153 218 165 133 816 182 162 218 165 140 867 19.2 5.6 0.0 0.0 5.0 5.9 321 345 350 6.9 1.4 6.3 27.9 6.9 6.4 12 6A 1 14 2 29 16 3 4 5 21 17 97 35 2 9 3 4 0 0 5 7 Mean 51 1 3 24 5 11 4 5 19 20 2 Mean 227 161 2 1 5 208 147 1 Mean 4 19 14 35 16 12 96 Wan 3 Number of red spiders Dead Alive Total % dead 79 345 163 49 270 1148 248 185 396 174 68 290 127 13.2 14.5 0.0 1.4 2.8 3.7 Plant Leaf No. No. 6 1 0 2 1 3 4 5 Mean 1 2 7 3 4 5 Mean 8 0 15 35 145 75 183 217 655 0.0 2.1 1.3 0.0 0.5 0.7 194 122 216 53 387 972 197 122 222 53 402 996 1.5 0.0 2.7 0.0 3.7 2.4 3.6 8 2 29 3 9 12 216 301 117 143 67 125 1074 224 330 126 155 364 1199 1 7 173 180 2 211 223 3 12 31 4 5 19 17 509 75 540 94 305 1342 Wan 56 1 2 3 4 5 Wan 3 0 6 35 142 74 183 216 650 1 Mean 10 1 0 1 5 24 4 5 9 Number of red spiders Dead Alive Total % dead 297 288 1256 10 97 5 144 0 0 33 48 673 123 63 209 636 Wt. mean 10574 Arith mean Standard deviation Standard error of mean Efficiency rating 107 149 123 63 242 684 11247 8.8 7.1 7.7 18.4 10.4 3.9 5.4 5.7 20.2 5.6 6.5 9.3 3.4 0.0 0.0 13.6 7.0 6.0 3. '52 6.643 0.939 74.0 25 For a long time there has been a need for biological evaluation of the efficiency of dust applications. Some thought was given this problem during 1941 when the standard deviation of the percentage kills was plotted against the rate in which various DN dusts were applied per acre. (See report to the Department of Entomology 191i1s page 14.) The standard deviation may be considered as mathematical evaluation of the manner a given set of variables will group themselves about the If it is assumed that a duster is working mean value of these variables. under optimum conditions, it should be capable of applying a uniform amount of toxicant on each dusted leaf. If this were trues then the standard deviation could be employed as a biological measure of efficiency of the duster. The standard deviation of percentage kill varies in scope with The greatest standard changes in numerical value of percentage kill. deviation of 0 and 100 percent kill. is 0 while the greatest possible standard deviation of 50 percent kill is 50. (See Figure 1). Table 1 shows the scope of the greatest possible standard deviation which corresponds to a given percentage kill. A simple formula for evaluating per- centage efficiency of on the basis of 100% is as follows. 100-BA (100) B-The measured standard deviation Where A-Greatest possible standard deviation of the measured % kill Example-A given dust was applied at a given rate per acre. The measured kill of a given number of leaves was 89%. The standard deviation of this measured kill was 13.05. Procedure-The greatest possible standard deviation of 89% kill is 31.288(a). The measured standard deviation was 13.05 (B) solving in the equation we have 100-131.3.0265 100 8 or 100-(.417) (100) or 100-41.70...58.3% efficiency : 001 : MOMOMMOMMIM 0 M 0 T IMMIMMIOMMIIMMEMMI 111111111MOMMIMMIMI MMIMENIMMOMMIMMIMOMM 111111111011111AMMIMMIMMFAIMME is a 8 0 17 O IIMM = 11 oppearommommum _.......fighimmoummimin MEM 0 MEMMIMMIMMOIMMIMMII MMEMEMEMEMMEMM UMMII INNIMMIMMIMMIMIMMIMMEMINIIMMI Emu MEMOMMEMEMEMEMEMEMMEM 1111111MMINIIIMIIIIErum muummonmskim...monm PRivipihmitr. 110 INIMINIMILO I iIkamispoporaimmi WEIN I II: PI 0 ttli IP UM le 11 MI- 1111111.11111111111111111111111111111 ur 25 For a long time there has been a need for biological evaluation of the efficiency of dust applications. Some thought was given this problem during 19141 when the standard deviation of the percentage kills was plotted against the rate in which various DN dusts were applied per acre. (See report to the Department of Entomology 19/41, page 14.) The standard deviation may be considered as mathematical evaluation of the manner a given set of variables will group themselves about the mean value of these variables. If it is assumed that a duster is working under optimum conditions, it should be capable of applying a uniform amount of toxicant on each dusted leaf. If this were true, then the standard deviation could be employed as a biological measure of efficiency of the duster. The standard deviation of percentage kill varies in scope with changes in numerical value of percentage kill. The greatest standard deviation of 0 and 100 percent kill. is 0 while the greatest possible standard deviation of 50 percent kill is 50. (See Figure 1). Table 1 shows the scope of the greatest possible standard deviation which corresponds to a given percentage kill. A simple formula. for evaluating per- centage efficiency of on the basis of 100% is as follows. 100-BA (100) there B-The measured standard deviation A-Greatest possible standard deviation of the measured % kill Example-A given dust was applied at a given rate per acre. measured kill of a given number of leaves was 89%. standard deviation of this measured kill was 13.05. The The Procedure-The greatest possible standard deviation of 89% kill is 31.288(a). The measured standard deviation was 13.05 (B) solving in the equation we have 100-13.05 100 .8 or 100-(.417) (100) or 100-1,1.70...58.3% efficiency 11111114] 0. I MUNI el 2111 Wag Allik di MEM 11, 11 NMI aril I WWII 1111411:111FAIMP11161111,111/1 1 1111111111111111111111111111111rniallailla amommilorammommiiii nimillipprimmamor_ musirmitam .110111113111 51 LU MEI MI 1111 "; 1 0 . . E 111111EMENE mEENIP m E®® posmom...0. Ensmum =mg= Esimisimimmiumminisimmoomm mmEgENENEE EIRIMMEMEMEmimmEMEMINEEMEMENIMEMEN 26 The standard deviations of the various percentage kills obtained during 1941 with three proprietary DN dusts at different rates per acre were accordingly converted into efficiency ratings. (See original data in 1941 report to Department of Entomologypages 117 to 181 of appendix). The resultant curve (Figure 2) assumes that of a straight line in contrast to that of the curvilinear nature of the standard deviation of the different measured kills. There is a significant correla tion between rates of application and efficiency rating. This is at variance with the original assumption that a duster should be capable of applying a uniform deposit on all dusted foliage and the corresponding correction coefficient should approach 0. The departure of theoretical expectations from measured observations in this instance may in all probability be credited from imperfect methods of field applications of dusts. The follcwing efficiency ratings of the 1942 applications are of interest. Treatment Treatment No. 1 DN Dust D-4 2 DN Dust. D-8 3 4 5 DN Dust D-4Frianite (50-50) DN Dust D-8Prianite (50-50) DN Dust D-4Frianite (25-75) 6 DN Dust D- 8--- Frianite (25-75) lbs. per acre 40 40 60 60 80 80 Efficiency Rating 81 66 58 61 62 61 The efficiency rating of 81 credited to 1)N Dust D-4 is decidedly higher than a similar rating of the same material apnlied at the same rate during 1940. The rating of DN Dust D-8 corresponds very well with that obtained during 1941. There is a marked lowering of the efficiency of the diluted DN 27 Dust D-4 over that of the proprietary dust. the DM Dust This does not occur with Two factors are apparently effecting these results. The one is differences in toxicity, the other differences due to decreased oil content in DN Dust D-8. The flowability of the diluted DN Dust D-8 was noticeably better than the proprietary product. It is believed that a reduction in oil content of the DN Dust D-8 from 2% to i or i% would add to the effectiveness of this product and at the same time eliminate much of the difficulty of feeding it into the air stream. 4. The commercial application of ammonium dinitro-o-cresylate dust Small plot testing of ammonium dinitro-o-cresylate dust in 1941 indicated that the material had some promise for hop red spider control. The data(See 1941 report to Department of Entomology, pages 20-29) showed 78 percent kill but the material was superior to dinitro-o-cresylate dust and the dicyclohexylamine dinitro-o-cresylate dust in holding red spider populations in check. Some difficulty was experienced in 19111 in atomizing the toxicant on to the carrier. Carbon tetrachloride, xyol and a number of volatile It solvents were tried but only a gelatinous mixture could be obtained. was believed that better mixing of the dust might result in better control of the hop red spider. A 1% dust of ammonium dinitro-o-cresylate dust was accordingly obtained from the American Cyanamid and Chemical Company. This dust con- tained talc as a diluent and was prepared by mechanically diluting the toxicant and the carrier. This dust was applied with the experimental hop duster on August 18, 19112 and data were collected August 22, 1942. 28 Table 1 Showing Greatest Possible Standard Deviation for Various Percentage Kills % Dead 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 5o Standard Deviation 0.000 9,949 14.000 17.058 19.595 21.794 23.748 23.514 27.129 28.619 30.000 31.288 32.496 33.630 34.689 35.707 36.660 37.563 38.418 39.230 40.000 40.730 41.424 42.083 42.708 43.301 43.863 44.365 44.899 45.376 45.826 46.249 46.648 47.022 47.371 47.697 48.000 48.280 48.538 48.775 48.989 49.133 49.356 49.507 49.638 49.749 49.839 49.910 49.960 49.990 50.000 % Dead 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 Standard Deviation 49.990 49.960 49.910 49.839 49.749 49.638 49.507 49.356 49.183 48.989 48.775 48.538 48.280 48.000 47.697 47.371 47.022 46.648 46.249 45.826 45.376 44.899 44.365 43.363 43.301 42.708 42.083 41.424 40.703 40.000 39.230 38.418 37.563 36.660 35.707 34.698 33.630 32.496 31.238 30.000 28.618 27.129 25.514 23.748 21.794 19.595 17.058 14.000 9.949 0.000 29 Discussion of results Complete data from this test are found on the following page. resultant kill measured 80%. proprietary DN Dust The This was significantly inferior to the D-4 and DN Dust D-8 at 40 lbs. per acre. It was again interesting to note that the sporadic kills (100 to 0 percent kills in different portions of the leaf) were again noted with this material. DN dusts. This same phenomenon occurred with the mechanically diluted It is probable that similar explanations may serve to explain this behavior. 5. Plant hormone sprays on hops Plant hormone sprays have been used with success by fruit growers in order to prevent preharvest drops of Ppples, pears, cherries, and other fruit. It was thought that these materials in either spray or dust form might prove of value to hop growers in prevention of hop cone shattering at harvest. More value might be enjoyed by growers using mechanical picking machines if these materials proved of merit. hormone materials on the market. There are a number of plant The one selected for preliminary tests was manufactured by the American Chemical Paint Company, Ambler, Pennsylvania and sold under the trade name of Fruitone. Chemical composition of this preparation is given as follows: Napthyl acetamid Napthyl acetic acid Wetting agent Talc Fruitone is also prepared in dust form. 1.1;14% 36% 5.00% 93.20% The formula composing the one used during these preliminary trials is given as follows: 1 .......,. ,.1..i..l.,. ,1Pmnmm..,-. ........E. ..CIMmlwin,,.....MMI 1. ,......... =...... IlnwaVkVIWEarlsyswlmmewm........, ...%=.1.4,an..==...m.z.,, IMINII=k TRERTMEI1T I (1.14(1, 114 INkIMILML 1.. PERRI 'OCP N=pmel. SPV)rv. 1,1/A 4 14% 01,1 5 841(0 0104111 6 (r11:1.'010 7. DI.S1 (.1" 0-4 a D-8 9 Claffl.. 10. DY 71N4 12 20 1.11060110C11 MI 23 144444000 0 MS00 14 LE. TArrittfOl 14 TOPAINot 01MMN6 I 1111,14.714,40L41,1,4 ' -,4i.kerEL 1941 EYTISITS Fop Field Day D1SCUSSIOL Cie RF IDR CC:7111CL 1941 T:op Field Day - 30 Population Counts 1942 Hop Red Spider Investigations East Farm Oregon State College Corvallis, Oregon Dust applied: Aug. 18, 1942 Data collected: Aug. 22, 1942 Material: Ammonium dinitro-o-cresylate 1%, Talc 99% Rate of application: 50 pounds per acre Equipment used: Experimental hop duster Plant Leaf No. No. 1 2 1 3 5 Mean 2 2 3 4 5 Mean 1 2 3 3 14 5 Mean 1 9 4 3 4 5 Mean 1 2 5 3 4 5 Mean Number of red spiders Dead Alive Total % Dead 220 130 187 79 24 190 25 21 33 644 190 42 188 218 70 708 195 130 218 99 149 791 269 224 317 103 211 58 913 98.2 41.0 76.7 90.0 43.1 70.5 15 67 14 11 205 109 202 229 3 73 818 92.7 38.5 93.1 95.2 95.9 86.6 4 110 3 29 37 64 27 160 7 95 212 145 188 688 1h5 233 179 213 66 836 144 15 62 90 184 15 82 0 5 37 139 198" 159 255 163 176 951 98.5 81.8 85.5 60.7 84.7 Plant Leaf Number of red spiders Total % Dead No. No. Dead Alive 144 167 6 1 6 3 4 5 Mean 1 2 3 7 4 5 Mean 1 2 3 8 4 5 077- can 213 422 166 1112 148 275 147 303 67 940 49 234 109 267 275 )9.T 1 181 39.7 2 509 84 135 160 315 179 90.6 73.9 100.0 218 103 975 64.1 55.7 70.0 67.6 64.2 97.7 Note: Sporadic kills observed on Portions of leaf had foliage. from 100 to 0% kills. Dust coverage good in nearly every instance. 9 3 4 5 Mean 1 2 10 222 258 64.9 64.7 301 12 0 269 434 166 1381 70.8 97.2 100.0 16 94 19 164 369 166 310 149 1158 7 82 218 0 5 3 4 5 49 31! 239 143 123 20 390 295 1 9 121 239 227 207 278 1072 93.I 78 91 88 16 90.2 74.5 88.6 97.7 45.0 81.2 100.0 97.9 76.2 68.5 93.2 3. 643 584 214 75 0 12 17 1423 456 147 231 1689 67 388 299 287 353 1394 9470 93 16o 41 0 4 81 219 2216 429 299 291 434 1613 11686 Mean 1t. mean Arith. mean Standard deviation .. Standard error of mean Efficiency rating 80.5 84 74.5 87.2 100.0 91.8 92.6 84.3 41.9 90.4 100.0 98.6 81.3 86.11 81.0 130.0 19.1.55 2.709 52.% 31 0.072% 0.018% 0.250% 14.660% 85.000% Napthyl acetamide Napthyl acetic acid Wetting agent Talc Frianite The Departments of Entomology and Farm Crops cooperated and undertook preliminary tests in the experimental hopyard, East Farm, Corvallis, Oregon. Later tests were undertaken at the Horst Hop Ranch, Independence, Oregon. A number of the difficulties and problems encountered are dis- cussed as follows: 1. Timing of application. been undertaken on hops. No work of this kind had been previously A spraying of apples 2 to 3 days before estimated time of drop gave best results. more rapid on hops than on pears. differences in rate of reaction. Reaction of the hormone was reported to he Varieties of apples and pears showed It was decided to spray both Fuggles and Late Clusters at various stages of maturity (bloom, burr stage, and early cone). 2. Concentration of material. One-half pound of Fruitone to 100 gallons of water was reported to give satisfactory results on apples and pears. and 1 The concentration used in the preliminary trials was both a lb. lbs. per 100 gallons spray on both Fuggles and Late Clusters at various stages of maturity. 3. Wetting of hops. The wetting agent in the proprietary product was not adequate in the wetting of hop leaves or cones. The nature of this wetting agent was not known but attempts were made to correct this difficulty. Aresket, resin potash soap, Ortho K light medium oil, Penetrol, Santex R-1001 Santex spreader-soap and tar soap flakes were among the materials tested. It was learned that tar soap flakes (2 lbs./100 gal.) added 32 to Fruitone (i lb./100 gal.) gave satisfactory wetting. Penetrol and nearly all the oil type spreaders produced burning on both cones and foliage. The compatibility of tar soap and the active ingredients of Fruitone are matters of speculation. 4. Application of material. Spray materials were applied to several plants in the experimental hopyard at Corvallis, Oregon by means of a small knapsack sprayer. a stepladder. The tops of the plants were sprayed by the added use of Application difficulties arose at the Horst Hop Ranch. spray machine was not equipped with an agitator. The One man was detailed to stir the spray mixture with a paddle but the efficiency of this method is questionable. The Horst hop duster was not sufficiently powered to reach the upper third of the hop plants. The college experimental duster was too high to travel through the yard in the same direction as picking machines. The greater portion of the hop cones remained undusted as a result of these difficulties. 5. Harvesting of crop. picking machine. The Horst hops were picked on the stationary This machine is equipped with powerful blowers for removing leaves and other undesirable material. Cleanliness of the hops varies with the calibration of this machine and with the foreman in charge of operations. The Fuggles were harvested when they were fully mature but the Late Clusters were very immature when harvested. This was attributed to the difference in rates of maturing of the various plants because of the severe inroads of downy mildew and the necessity of cutting back plants a number of times throughout the season. Some difficulty was experienced in preventing growers from picking the treated plants in the experimental :rard in Corvallis. As a result of 33 this eight of the fourteen plants were lost. Discussion of Results Samples of the treated hops were taken from the Horst Hop Ranch and the Experimental Hopyard and examined for percentage broken cones, percentage seeds, percentage leaves and stems, and percentage strigs by the Department of Farm Crops. These results are tabulated on the following page. Fuggles are known to shatter more easily than any of the common commercial crops. This is evidenced by the difference in percentage broken cones between Fuggles (Mean 83%) and Late Cluster (Mean 21%). Some of this difference, however, must be credited to the difference in maturity of the two varieties at time of picking. There were few replications of either dust, spray or check hops from There is probably no significant difference between the the Horst Ranch. samples. This was largely to be expected because of wetting, mixing appli- cation, and harvesting difficulties would have masked any differences which might have occurred. The samples from the experimental yard were hand-picked and separated from the total picked lot of hops. harvesting treatment. vesting treatment. treatments. They, therefore, did not receive normal Both treated and check hops did receive normal har- Both treated and check hops did receive comparative Replications are not sufficient for speculation but there may be some difference in reduction of shattering of Fuggles. There is some question regarding the ultimate value of reduction of hop cone shattering. Accomplishment of an objective of this type is filled with difficulties, some of which were encountered in this preliminary investigation. It is believed that sufficient information has been obtained 314 The timing of sprays should to warrant larger scale tests in the future. be made at or about the time hops are in the burr stage. this time Spraying after probably lessens the possibility of covering the bracts with a coating of spray. Considerably more work should be carried on in order to solve some of these problems. 6. Continued work on red spider control on strawberries. Investigations were first undertaken on red spider control on strawberries in 1940. These studies were conducted on the strawberry patch of Mr. E. A. Jones, East Farm, Corvallis, Oregon. The plants were of the Corvallis #12 variety (a late maturing variety), and were planted in hills three feet by three feet. The plants were very heavily foliated and often exceeded 2 feet x 2 feet x 2 feet in dimensions. It was learned during 1941 that the common knapsack dusters were not satisfactory in depositing dusts on the undersurfaces of strawberry foliage in a planting of this type. The Department of Agricultural Engi- neering cooperated in solving this problem and a satisfactory unit was finally developed. (See report to Department of Entomology 1941, pages 29-35). It was also learned during 1941 that DN dusts could be applied to strawberry foliage with safety if temperatures did not exceed 70 degrees F. for at least three days after application. Walnut shell flour was found to be poorer than frianite in effecting satisfactory coverage on strawberry foliage. It was decided to carry on additional tests in 1942 in order to verify the 1941 results. Red spider populations were checked throughout the winter and early spring. It was learned that this pest overwintered on 36 Fruitone Spray and Dust Experiments, 1942 Samples from Horst Hop Ranch Variety Late Clusters Treatment 1# Fruitone 2# soap 100 gel. water Check - No Treatment State of maturity Variable (prebloom, bloom, burr and cones to 1 in. long Sample No. % strigs 17.0 16.1 14.1 9.1 6.9 7.7 7.9 18.0 31.5 9.0 19.5 6.9 13.1, 11.14 7.7 10.0 8.2 15.9 9.5 12.9 8.1 9.7 9.7 77.0 90.0 83.0 83.3 13.7 12.5 13.0 13.1 0.5 0.6 1.5 0.9 11.9 9.0 9.9 10.3 81.0 88.0 83.0 1.5 6.4 1.7 3.2 8.7 7.9 814.0 14.2 13.1 12.8 13.4 81.5 81.0 10.0 9.6 2.7 2.3 12.5 9.8 2.5 13.7- 53.0 24.5 12.3 10.6 2.h 1.0 9.1 10.0 38.8 11.5 1.7 9.6 77.0 67.5 7.1 7.6 0.1 0.3 11.1 12.0 12.3 7.4 0.2 11.6 1 28.0 7.6 0.3 11.0 1 32.0 7.2 5.0 9.8 3 Ave. 2 Ave. Cones nearly mature and leaves 3.6 5.7 7.5 5.6 2 3 Fruitone Dust % stems % seeds 19.0 24.0 26.5 23.2 1 1 As above % broken cones 1 2 3 Ave. 9.3 Fuggles 1# Fruitone 2# soap 100 gal. water 1 Ls above 2 3 Ave. Check - No Treatment 1 As above 2 10.1 8.9 '.L.8 3 Ave. 0 Fruitone 100 gal, water Burr stage 1 2 8.13 3 Fuggles Ave. Check - No Treatment Burr stage 1 2 3 10 Fruitone Late Clusters 100 gal, water Burr stage Check - No Treatment Burr stage 36 strawberries. They began to increase at an alarming rate by the middle of May and it was decided that further delay might result in crop injury. Four proprietary dusts (DN Dust D-)4, DN Dust D-8, DN Dust D-7, and DN Dust D-3) were applied on May 14 and 16. The decision to time the dust applications was made difficult by the elimination of long range weather forecasts by the United States Weather Bureau. Labor and travel diffi- culties also prevented the collection of a great amount of quantitative data. Randomized samples of fifty leaves were taken before dusting and from each treatment after dusting. These data are given on the following page but may be summarized as follows. Treatment Population of red spiders Before dusting After dusting 6.9 DN Dust D-4 100 lbs./acre 93.1 DN Dust D-8 100 lbs./acre 93.1 5.3 8.2 DN Dust D-7 100 lbs./acre 93.1 DN Dust D-3 100 lbs./acre 9.5 93.1 Note--Nalnut shell flour used as carrier with DN Dust D-3. as carrier in other dusts. % Reduction 92.6 94.3 91.2 89.8 Frianite used Discussion of Results Sixty six (66) plants were examined carefully one week after dusting. Foliage injury at this time was limited to 17 leaves. Most of tie injury occurred at the end of the rows where plants received excessive dust as a result of turning of the duster. These plants may safely be estimated as averaging 200 leaves and the foliage injury is therefore approximately 0.13%. This is not considered to he of economic importance. Satisfactory controls were obtained with all of the dusts. D-3 may be considered as inferior to DM Dust D-8. DN Dust This difference may 37 probably be attributed to difference in diluents of the toxicant. Walnut shell flour (diluent for DM Dust D-3) was reported as inferior to Frianite in depositing dusts to strawberry foliage in 1941. Early in the season the experimental duster was used to treat this strawberry patch with i% Rotenone. Talc was used as the diluent. controls were obtained with this dust at 35-40 lbs./acre. Excellent Talc at this time was noted to give excellent coverage on the undersurfaces of strawberry foliage. DN dusts as used did not effect satisfactory controls of the spittle bug. It is possible, however, that by the use of talc or diatomaceous earth as a carrier that more effective results could be obtained. Frianite and walnut shell flour did not tend to be absorbed by the spittle of the spittle bug. Rotenone and DN dusts have been found to be compatible with each other and it is possible that i percent Rotenone could be incorporated into the DN Dust and that both pests could be controlled in one operation. Talc has not been used as a carrier in DN dusts because of its variable pH. It is believed that treating and acidifying talc prior to atomizing the dinitro- o- cyclohexyl phenol would result in a satisfactory product. Talc, because of its bulkiness and ability to adhere on strawberry foliage, would make it possible to treat strawberries at the rate of 40 pounds per acre. Tf costs of preparing this dust were similar, this might result in reduction of material costs from 410.00 to $4.00 per acre. Late in the 1941 season, two border rows of the strawberry patch were allowed to stand after all remaining rows were topped. that red spiders may be induced to concentrate on these rows. It was thought A very heavy dusting of DN dust was applied in the middle of July and severe foliage injury resulted. Practically 100 percent kills of red spiders were noted. 38 These dusted plants were studied during 19112. No permanent injury resulted from this severe foliage injury but there was no notable reduction of the 1942 red spider population. It may be concluded that red spiders may be controlled on strawberries with DN dusts (DN Dust I1-4, DN Dust D-8, DN Dust D-7, and DN Dust D-3). Extreme caution must be practiced in the use of these materials and DN Dust D-7 will probably prove to be safer to use then the other dusts. Temperatures must not exceed 70 degrees F. for at least three days after treatment. Seasonal conditions are favorable during April and May for the use of this material. The use of long range weather forecasts by the United States Weather Bureau will aid greatly in this respect after the termination of the present war emergency. The accompanying letter by Mr. Jones will serve as a testament to the value of red spider control on strawberries. COPY December 14, 1942 Don C. Mote Entomology Department Campus Dear Mr. Motes In reporting to you on the experiment Mr. Morrison did from your department on Red Spider for strawberries at my farm, I am very glad to say that I think it was a success. Taking my patch for a three year period, with the first year not being dusted and next two years with the dust being applied, I figure the increase in tonnage and the price I received for the berries brought me about $85.00 per acre, You perhaps know the cost of the dust so as you can arrive at what the net profit would be. I wish to thank you for your support in this experiment. Also, I wish to thank Mr. Morrison for his effort for he certainly was on the job. He gave the experiment careful study and worked hard making mapytrips out here after working hours in the evening. Thanking you and your department again for the interest in the matter. Yours respectfully, E. A. Jones Route 3, Corvallis, Oregon 39 Common Red Spider Population Counts East Farm Corvallis, Oregon Cooperator: E. A. Jones Data collected: 5/19/42 Date dusted: 5/14-5/16 Host plant: Strawberries (Corvallis #12) Rate of application: All treatments at rate of 100 lbs./acre Leaf No. 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Red spider populations - 3 days after dusting Original red spider populations DN Dust D-4 DN Dust D-8 DN Dust D-7 DN Dust D-3 29 1. 89 12 9 11 3 9 44 3 25 8 168 0 33 6 1 6 56 5 195 1 14 0 5 0 88 8 9 5 0 0 11 15a 9 0 16 16 3 97 0 0 11 41 13 0 13 7 73 7 0 0 16 so 5 0 119 5 7 3 29 27 15 0 37 11 8 96 7 5 2 1 32 13 49 0 6 19 8 82 1 63 3 5 7 0 8 2 7 58 1 195 5 0 9 8 0 2 16 218 8 0 41 5 73 6 2 0 45 9 8 22 29 5 5 0 0 0 5 73 46 91 51 47 35 44 26 27 28 29 30 31 32 33 73 16 34 35 36 37 38 39 ho 41 t. 14 9 9 7 9 6 5 1 8 7 9 9 5 31 8 3 9 21 7 2 69 19 6 0 89 155 5 0 1 12 6 0 0 346 0.0 5 0 0 14 17 5 1 9 16 66 4651 93.1 27 0 1 0 0 1 0 5 14 216 258 97 gglinina13(39.941 control 5 3 1 83 98 47 89 Mean 8 18 12 73 Total 0 0 97 46 188 95 79 189 42 43 44 45 46 47 48 49 50 0 0 0 -6.9 6.87 0.971 92.6 1 5 1 0 a 1 3 267 5.3 g:g6 94.3 11 13 0 16 0 0 19 13 15 6 12 9 408 8.2 8.43 1.12 91.2 3 6 0 0 0 5 19 37 0 0 27 19 21 8 0 5 9 2 31 16 29 1 7 0 0 0 474 9.5 10.q5 1.49; 89.8 40 Phytocidal studies of DN dusts. 7. Several specimens of s very minute mite were brought to the Department of Entomology during the middle of June. These were submitted to the United States Department of Agricul- ture, Bureau of Entomology and Plant Quarantine, at Washington, D.C. were identified as the broad mite Hemitarsonemus latus Banks. They This TRS a new record for Oregon but they had been take: from Bouvardia by C. F. Coucette at both Sumner and Tacoma in 1933. This species was causing damage to cucumbers in the greenhouses of Chase Gardens, Eugene, Oregon. The undersurfaces of the leaves were stripped of hair filaments causing the leaves to become very papery and assume a glazed appearance. Several dusts were applied on May 28 and examination of the results was made May 31. The following results were obtained. Treatment DN Dust DN Dust D-4 DN Dust D-8 Din:Itro-dust Sulfur Check No. of leaves counted No. of living mites 10 10 10 10 10 20 Mean mites per leaf 49 57 75 5 6 185 205 1603 19 21 80 8 Severe burning resulted from the dinitro-dust application but all other materials appeared safe on cucumbers. and no dead mites were found. The results were not conclusive These mites were not web spinners and may have fallen from the dusted leaves. Attempts were made to pick repre- sentative young leaves for examination after dusting because young leaves were noted to be most heavily infested. These mites were also found attacking young tomato plants in the greenhouse. )41 Attempts to establish a culture of this mite in the Entomology culture room for additional investigations resulted in failure. The James Gardens, Good Pasture Island, Route 4, Eugene, Oregon had planted a variety of herbs and condiments for experimental purposes in 1942. A number of these plants became infested with red spiders and an Applications attempt was made to determine their tolerance to DN Dust D-4. were made on September 22 and the plants were again examined October 1. Temperatures reached a maximum of 87° F. on the third day after treatment. All other maximum temperatures were below this point. The following is a summary of these observations, Plant Degree of injury Lavender (Lavandula vera) Tobacco (Niaargiii-gigi7a) Henbane (Hinscynus niger Sweet Marjorum (Majorana hortensis) Sage (Salivia officihklis) Thyme (Thymus vufgaris) BelladoriaTftrupa belladonna) Jimson weed (Datura stramonium) Foxglove (Digitalis purpurea) None None Slight foliage injury None None None None None Slight foliage injury Red spiders were more populous on Nicotina glauca than on any other plant. A few were found on Belladonna and Henbane and none were found on lavender, Sweet Marjoram, sage, thyme, Jimson weed, and foxglove. The above information may prove to be of value at some future time if any of these plants become grown commercially in Oregon. It is believed that all of the above plants could be safely treated with DN Dust D-4 or DN Dust D-7 without excessive plant injury. Methods of application of these dusts to the plants would require some attention, MIME 111111.111111.1111.1 mmiammosimiummo NFL Itrollrolitil!glii ;7. Eel 11:10 .:011 1: I 11E.1 51 7 41 I NE11111E AMMINIMENI Pal E iromorm ,..Erri raus nomminno NENE IIMIENIFAIIIIIMENEMFIMENMEMEN1 MEI =ME= FAIMMENE EINEMENUNIMM 11411, .111/11ErlIMINERNIIIIM 11111111151111111111111E1 MIRIPPIPPIIPNIONUIIPIPPPPNIMPIIIIME ILLOICLIILLigliAUMILligilitLINLMINE KC Mil 1 IMMINUMEIMINIENNENIIIMIEMEN siramaillIg, ;1 :I:I 711 NE= am EIMMENEMEIMI MEM A EssuimiguAn Iu eir MI NE RE Mir El 8. Completion of chemical studies of DN Dusted hops. An original study was made during 1940 of the effects DN dust and DN Dust D-L might have on the physical, chemical and brewing qualities of hops. The results of this study have been included in the report to the Department of Entomology (pages 234-240). It was thought advisable to continue the study of the possible effect these dusts might have on chemical qualities of hops after they had been allowed to stand in storage. Chemical analyes were accordingly made by the Chemistry Department of Oregon State College after samples had been stored at room temperatures for a period of two years (10/7/42 to 10/17/42). The results of the original and final analyses are tabulated as follows. Percentage composition Undusted hope DN D-L dusted hops DN dusted hops 1077/40 10/17/42 10/7/140 10/17/742 10/7/40 10/17/42 Alph*esin 7.11 Beta resin Hard resin Total resin Preservation value Moisture 11.3.5 1.17 19.64 109.00 10.7 6.52 0.32* 12.86 8.95 1.42 5.78 15.05 20.80 33.00 108.1 6.8 9.3 * Old hops always giite too low values for Alph4esin. probably contain more 1% alpha resin. 0.20* 10.52 5.00 15.72 37.1 6.4 7.05 12.00 1.30 20.15 110.5 10.7 0.63* 11.04 5.49 17.16 43.1 6.8 None of the above Above analyses made on moisture-free basis (Original analyses in 1940 report not made on moisture-free basis). Discussion of results Preservative value is computed from the formula P. V resin !- Beta resin). 10 (Alpha The difficulty in analyzing for Alpha resin in old 3 hops combined with its importance in determining presertrative value indicate that there is probably no significance between any of the above samples. DN Dust D-4 and Di Du4st-ta-TI therefore, be used on hops without fear of lowering the physical, chemical or brewing qualities of hope. report) (See 1940 I3 SUGGESTIONS FOR FUTURE WORK The status for continued work on hops depends largely upon the emphasis placed upon hop growing and its importance to the war effort. The following phases are considered worthy of additional effort if and when circumstances warrant their study. 1. Continued study in improvement of dusting machinery. More efficient dusting would probably make it possible to further reduce amount of toxicant per acre. 2. Study of the various carriers and diluents for toxic materials in an effort to reduce amount of toxicant per acre. 3. Continued seasons] history studies of hop pests would be of value in giving added information on the biology of these pests. 4. There is need for the development of a simple and rapid method of measuring populations of red spiders and hop aphis. There are a number of techniques which have been developed on other plants and it is possible that one of these could he adopted for hops. The technique should be simple in order that growers could use it in timing of their control operations. There is decided need for extension work among hop growers. This work should include instruction on use of mechanical equipment, timing and method of applying dusts. 6. Research should be undertaken to determine the proper timing of DN dusts. It is possible that early application of dusts may prevent serious infestations of red spiders. evidence to support this possibility. There is some 7. Ammonium and guanadine salts of dinitro-c-cresol still have promise for control of red spider. There is need for tests of these and other dinitro compounds, which have been atomized with the carrier. 8. Preliminary tests to determine the value of plant hormone sprays on hops were not promising. Studies should be continued, how- ever, in order that some of the problems in applying the materials could be eliminated. 9. The possibility of using talc or diatomaceous earth as a carrier for DM dusts on strawberries should be undertaken. It is possible that these materials would reduce the amount of material neces- sary for commercial control as well as mike them effective on both red spider and spittle bug.