ANNUAL REPORT of
HOP INVESTIGATIONS
(oAES 36, cReS)
Distribution of Copies
1. S.N.Brooks,
Farm Crops Dept., O.S.C.
2, Industrial Crops
Section, A.P.S.
3. Director, O.S.C.
Agri, Expt. Sta.
h. C.E.Horner, Bot.
and Plant Path,
Dept. O.S.C.
Chem Dept., O.S.C.
5. S.T.Likens, Agr.
6, Industrial GYops Section, A.R.S.
'
Farm Crops Dept., O.S.C.
Vi le baiter S. Sarney
Sa t Likens, Chest
26
27
Oft (
36) T
HOP DISE
C. 1. Horner
ON OF FUNGICIDES AS DUSTS OR S
CONTROL.
]$S8 Field Trials with Streptomycin
Streptomycin Absorption by Field Treated Hops
Soil Treatment for Root Rot Control
S
CRe5-3 (OAES 36) THE usibLOPMENT OF PIE
TECHNIWES RELATIVE TO
INVESTIGATIONS ON HOPS.
it
USE AND L
DISEASE A
CATIONS 1IAT
AND QUALITY IN HOPS
52
53
) CHEM
OF HOPS*
S. T. Likens
STIGATIONS RELATIVE TO THE
A. Evaluation of Strobiless Alpha and Beta Acids
B. Evaluation of Strobilost Hop Oils
C. Evaluation orStrobiless Storage
ATION
67
67
72
76
# Planting Plan
Utica Testing, Nenorandan of Understanding
...station Testing, Preesdares for Cream hinieal Stripping and Sastering Trial s IrriptIon.440441ity:Seprtsant s o f t itterellis Acid on ?sales
Sorority of Pruning Study rags Data Levis Brows Sondless Yard
Stara. Data @silty Trial, 195748
National Bsp Rosearoh Conmitte*
957-0
fir.
the VIDA ixperiment tie* at Prosser, Washington of California. For sane in reviewing, descriptions, senmeries and sammariming data are ineleded in the fore part of the report by specific line project.
Additional data uhioh are important enough to include as a orator of permanent record are included in an appendix at the end of the report.
Knee lame of the line projects are eomducted eeope personnel located at Oregon State College, more than one investigator may have work reported in one line project report. An attempt hap been made to give each investigator full credit for his contribution to the year's work.
The work reported in this report is directed along five lines involving studies on hop culture, breeding, disease and quality investigations.
The studies on hop culture and breeding are cooperative among the agronomist, the chemist and the plant pathologist. The studies on disease control are
Chemical studies are 000perative between conducted by the plant pathologist the Chemist and the agronomist on certain phases, and other phases are con.
ducted independently by the Chemist.
A fifth type of investigation inoludes studies of field, greenhouse and laboratory techniques relative to the breeding, disease and agronomic lines of endeavor. Any OM of the project leaders may have work reported under this line project from time to time.
In addition to the support given to this work by public agencies, certain annual grants of funds have been contributed by the Brewing
Industries
Research Institute for partial support of the work in Oregon. Begiming in
1959, such support will come from the United Brewers Foundation instead,
point out the
*web personnel and of Mario., Ins. last 'interim Salm, On as liaison between the rooms* groups in the growers The eemmittee was to alt as a in bringing research results to the grower and as a body to work in that different areas to eliminate us-noodod duplication
Tho ionsitteo was named the National BO, Research Committee and was mode up as follows,
Name and organisation
3. N. Brooks Chairman
B. BvDepartmont of Agriculture
C. B. Skotland
Plant Path., W
C. E. Homier
Agent (Plant Pat.), ORD ARS and Ore. Agri°. Expt. Sta.
W. G. Golden, Jr.
Farm Advisor, Cabo. Agric. Rzt.
R. R. Romanic,
Plant Path., Idaho Agri°. Expt. Sta.
Washington
Oregon ornia
This committos met twice during 1955 and will meet again
Sacramento, California on Feb. 4, 1959.
On February 5, 1959 it will present current research findings to the Bop Growers of America, Inc. at their annual convention.
Bop production traditionally has been subject to wide variation in total acreage, total producrtion and center of production.
The hop industry suffered a severe recession in the early 19504 due to low prices.
Since 1956 there has been considerable improvement in this situationl and the industry is ones again on a sound basis. A oontinuation of expansion could however,
n
3,951 27,700 33,1 00 1,473 lrket die hh9 149,514 40,135 h5,1s0
T train
Division, USDA
7 ly
Aug.
Sept*
534
54.6
73.0
4
864
75.4,
!early
total
!early
scan 64.1.
63.6
55.8
62.8
69.2
73.3
80.9
81.5
76.7
366
40.8
46.7
.5
55.9
48.6
434
38.0
35.,
.3
.3
37.6
404
45.2
494
52
524
494
14 40 las e
44.3
4,041 7
59
63.6
70.3
62
7r.
avg
3.114
2.01
3.54
3
.7
51.5
57.1
61 .8
46
4
67 4
63.0
8.15
8008
I+55
3,66
1.12
2.91
.02
2.4a
6
3.67
242
1.71
1.44
38.42
38.39
42.7
53.6
Month
Reldnuld.d. Evepora.
SOAK 0) tlen (ln.)
6yrs
1958 Avg.
tr
Noptly.
cloudy cloudy
Me.
rainy
27 yr.
18 18 18 18
1958 Arg 1958 yr. 1958 yr. 1958 yr. 1958 yr.
Avg.
Avg.
Avg.
Avg.
A velGoity
(MPH)
6 yr.
1956 Avg,
1958
Apr.
May
81.4 81.2
1.105 2.684
7 8.8
19 .3
7646764 5.201 4.103
12 2.0.0
12
1211.4
June 80.0 76.3
4.514 4.679
9 8.3
July 69.6 70.5
8.287 6.551
22 17.7
13 124
9 10.2
Aug* 65.4 73.2
8.313 5.834
17 16.0
Sept.
5 8.8
761A0.2
4.795 3.865
6 14.4
13 9.1
3 9.0
22 13.8
2.37
244
7 us
9 10.5
495 1422
7 9.1
14 9.2
1.06 1.27
1 30 1 2.7
1.75 1.52
2 5.,9
2 3.0
1.80 1.55
9 5.6
2.15 1.68
Yearly total
TearlY74.9 76.3
mean
91
73 75.2
71 660
25 40.5
57 44.8
1.68
1.56
order to data are all than being separa and involves the three to more meaningful, agronomic hemical and disease byevaluation trial within this line project rather three sections of the report.
Genetic diversity for the breeding program consixte four widely grown varieties and approximately 75 introductions of which only a few remain.
Many of them are new represented only by first or later generation progenn and some have been discarded entirely.
gone of the varieties or introductions have exhibited immerity to downy mildew.
Some of them possess acceptable quality and agronomic characteristics, but none of them are sufficiently satisfactory in all important features that improvement through breeding is not desirable.
Due to resent reports from Wye College in England that varieties of
American parentage exhibit resistant to downy mildew crown infection, a now approach was initiated into the breeding program.
C. E. Horner collected living specimen* of the wild hop native to Utah last spring.
Clones of these plants were established in the breeding block and will be used as breeding MRS terial in future years.
Accession numbers will be assigned to the clones when it has been determined haw many of them will live.
Since its inception, the hop breeding program has been confined generally to inter varietal crossing involving both domestic and foreign varieties.
A modified recurrent selection scheme has been in effect since 191s8.
Selection has been based upon resistance to disease, primarily downy mildew,
planted
Trials were placed in the o & an. ,ttea pt to duplicate of the egomania tion phases of this project were expanded during the year.
each of 1034, 1014 1034,
trials by °. I. Nelson (Prosser) in the Taking VaLlay.
Dare Streams of Hibton, Laverne Aires of T nigh and
Lawrence Brullotte of Memo City, Washington.
Approximately 100 hills each of 112 1284 135 and were planted in each of three trials in the Willamette Valley.
Trials were placed with Ray Kerr of Salem and Ed Crosby and Louis Schwaben*? of Woodburn,
Oregon.
Clive Cook and Hollis Manley, County Extension Agents, cooperated and will continue to cooperate in this prograa.
Plans are being made to establish one more off - station trial in this area.
During the winter of 195148, hills of each of the eight lines represented in the Oregon and Washington off-station testing program were planted en the Ranch of Peter Bradley of Elk Grove, Califernia.
Due to winter flooding conditions Shortly after planting, considerable stand loss was experienced.
Additional cuttings will be sent in February 1959 to W. G.
Colden
Farm Advisor at Sacramento, who is cooperating in the program.
In December 1958 10 cuttings of each of the afore-renti sent to R. R. Romanko at the Parma Branch Experinent Station in Idaho.
plant them in a cooperators yard and supervise their future evaluation.
Lines which are being released to growers are done do under an informal memorandum of understanding.
A copy of the memorandum can be found in the Appendix of this report.
In the appendix also is a copy of the procedures
.5
had resulted fret what wee presumably
Cross between Late Gloaters and a sex-reverted Leto Cluster MU total of 59 progenies available tOr study in 195%
The growing season at Corvallis was unusaally hot in 1958 and was particularly hard to the breeding pi ogrwa Maar of the foils flowering branches were blasted or otherwise kondered non-functional under the parchment bags. As a result only about 15% of the crosses mods produced seed.
This is about one.third as many as are good in the average year.
Group Is 54 crosses were attempted among resistant and susceptible male and female plants in order to obtain seedling progenies on the crosses used in a downy mildew inheritance study set out last year.
Only sight of these crosses set seed, and the whole group will be re-made in 1959.
It will be desirable to have seedling reaction on these progenies as well as mature plant reaction.
Group III Six (grosses were repeated (25 attempted) involving parents that had produced downy mildew resistant seedlings several of which had gone into the 1957 nursery or elss bad had an insufficient progeny test in 1957,
These crosses were the same as the Group II crosses in 1957. They were 58001, 58010
58012, 58034, 58053 and 58057.
Group ITIs 16 crosses were made (104 attempted) between female plants which had produced several resistant seedlings each from open-pollination in 1956 and 1957 and males which had resistant progsrq in either 1956 or 1957. These crosses wore,
58002
58013
58032
58033
58042
58043
58044
58045
58047
58048
58049
58051
crosses, obtained
5$036 a prover test en will be salso.
197 in 1959.
se plants along with 219.4 should be tested further
Croup Ts Open- pollinated seed was dollected several relatively new females in the breading block to provide a mildew evaluation progeny test On the basis of this test these nee lines will be saved or discarded from the breeding program.
The lines were!
104-1
114.3
2154
301.1
3013
No OP
302.1
302.3
306.1
306.2
306.3
a obtained from 504.1, and this line will have to be tested another year.
Open.polli
obtained from 18-8 (C 19113) which is the same as 128-I now undergoing off-station testing as a seedless or sterile high.yieldingo high analysis lino.
This genotype set seed this year rather prolifically (for it).
It will be of interest to see what its progeny look lila
Open.pollinated mead of Leta Clusters was obtained from Robert the Parma Branch Experiment Station in Idaho. This seed resulted
fawn polliattice by presumably iscrormirsed Leto Gluing.
for mcgAgation for Walt reastien.
121-2 and 1 s23,40 were made in 311 (I 3S001, Brown* of starting a bask Grossing program current parent. Mae lines 1212 (G 19062 10, WO
Itrowers
190140
(C 19122 N) are vigorous, mildew resister& lines. Those lines will be in backeroowing Programa with Brewers Gold, Late said lerly Clusters lallertma and Backs in an attempt to incorporate mildew resistance and vigor into lines resembling the domestic and foreign varieties in quality. The seeds were put in moist sand and placed in storage at Xer. on January 5, 1959 are they will remain until February 16 at which time they will be renaved to the greenhouse for germination. The resulting seedlings will be subjected to downy mildew screening and selection.
5110011
106
) Q 500,1 s 32.4 t 313
3
C
) C 19034 x 321.2 (525) C 3.9062 X
50005 106 (506) 0 19032 x 2214 (1s25.1) C 51101
X
58006* 106 (506) 0 19032 z 3174.1,, 2 (317) C 1904 x
0007 41 106 (506) C 19032 z 321.a. (325) c 19049 x
58006 * 106 (506) C 19032 x 121.1, 2 (225)
C 19040 N
58009 * 106 (506) C 19032 x 52141 5 (126)
C 19010 M
58010 108 (506) C 19033 x 124.3 C 19183 14
58011 114.3 (5144) C 52020
58012
58013
203 (103) c 19022 x 1418.1, 2 (219)
1 19006 M
203 (403) C 19022 x 119.S-0 19180
7
580111 210 (110) C 19027 x 3174 (318 -2) C 510514 it
58015 * 212 (412) C 19028 x 5214, 5 (126)
C 19010 Di
58016 215..3 (4154) C 52012 x 0.1),
52017
58018
58019
301.1 (301-3) C 50040 x 0.12.
301.3 (3014) c 50019 z 0.P.
302-1 (302.3) C 52001 z 0.1)
58020
58021
58022
58023
58024
58025
58026
58027
302.3 (302.1) C 51065 x 0.1),
306.1 (3136-3) C 51105 x 0.r.
306.2 (306.2) 0 511014 x 0.13,
30603 (306-1) C 53203 x P.P.
307 (307) C 19020 x 2194 (1422.2) c 51061 m
307 (307) C 19020 x 317.4 (3182) C 51054
307 (307) C 19020 x 518.5 (120) C 52041
307 (307) C 19020 x 519.2 (121) C 52042 N
11
58046
58047
58048
58049
58050
58051
58052
58053
58054
58055
58040
58041
58042
58043
58044
58045
58030
58031
58002
58033
58034
58035
58036
58037
58038
58039
(
)
51021 x00,
(311) I 19001
121 -2 (525) C 19062 m
311 (311) I19001z121 -].
2 (225) 0 19040 m
314 (314).0 19076 3: 521A, 5 (126) C 19010
314 (314) C 19076 x lama C 19183 )1
401 (201) c 19022 x 4194 (422.1) 0 51060 m
401 (201) c 19012 x 5214, 5 (126) c 19010 m
402 (202) C 19013 x: 419-5 (222) C 52048 m
404 (204) c 19014 x:317.4 (3182) c 51054 m
404 (204) C 19014 x 419-5 (222) c 52048 m
404 (204) c 19014 x519 -2 (121) C 52042 M
405E03 (205-1) C 54010 x O.P.
414 (214) C 19018 x 518-5 (120) C 52041 m
422 I 56002 x 123.3 C 19182 m
501 -3 (101.1) C 50008 x 320-4, 5 (324) C 1908 11
501.3 (101.1) C 50008 x 417.1, 2 (217) C 19036 m
504-2 (1062) C 54003 x 320,4, 5 (324) C 19048 m
504,2 (1042) C 54003 x 419-1, 2 (221) C 19037 m
504.3 (104-1) C 54002 x O.P.
507-3 (1071) C 54005 x 217.1, 2 (417) C 19050 M
507-3 (107-1) C 54005 x 319-4, 5 (322) c 19046 m
509.1 (109-3) C 5004 x 320.4, 5 (324) C 19048 m
5104 (110 -3) C 52038 x 0.P.
511-3 (111.1) c 53007 x 318-1, 2 (319) C 19043 m
511-3 (111-1) C 53007 x 418.6 (220) C 52047 H
514-2 (114-2) C 53013 x 521-2 (125) C 52045 M
7-8- C 19102 x 517-1, 2 (117) C 19008 H
18.6- C 19113 x O.P.
12
* 0 see repsatsd for DM inhoritanoe study*
eels.
tlisugh a complete motel's not ebtainedit is felt that suffieient
2 x2 and 3 X ) eombinatiers will be smailable to give congiderable infereation on.downy mildew inheritance ?hire will also be various 2 z 3 and tx combinations available ter study immolvingthe following parents (oode Immhers)1
Resistant males are X, T Andl
Susceptible males are R, Sand ?
Resiatant females are Ciand D
Susceptible females are Es F and
Segregation and trap/env distributions of course, can be studied o e cross individually.
The 22 erasses and of the parents were planted in a l0 z 10 triple lattice experiment.
The plots were three hills spaced four feet apart in rows eight feet apart.
One plot of each parent and four plots of each cross were included in each of the three replications.
The main purpose of the triple lattice design was to insure that the plots of each cross were separated as well as possible with respect to each other. This also insures that no parent occurs In the same 1a -plot block more than one time with any other pare
It is believed that this will give mere realistic estimates of genetic and environmental variance when the area occupied by the experiment is taken into consideration.
The variance of the parents will be used to estimate environmental variation.
The triple lattice design is, therefore, mostly for the purpose of field arrangement rather than for statistical analysis.
11 crosses had sufficient progenies that an additional three replications could be sat out which included four 3hill plots per rep. along
15
A trial eon be t
As a re of
Pro of 36 individuals
Prods of 72 individuals have nine clones eash the
U of the oxv
11 of the Gros pt for parents as 0 and represented WI 13 clones total, in six replications.
It is re
However, in view of the area and expense of hops, and in view of the fact that the popalations are extrmeely small.
ed in growing large populations r knows of no ether like experiment, it is considered to be adequate for prelininary data on inheritance in hops.
The results which will become available in future years will be recorded and suimarimod using IBM facilities.
Group II. repeat-- of 1956.3,957 progeny testedArosesst this
Seedling progenies were obtained from 11 of the 19
These aro:wee-mem sews
*57042
57047
57136
57138
57145
57184
57186
57196
Cross No. 57042 had exceptional seedlings.
57218
57215
57256
The performance of the asedlings from these crosses can be obtained in the section of this report dealing with "seedling reaction of 1957 crosses to downy mildew".
11 crrosse s of ro mel
This grOup contained crosses made between openpollinated progeny tested females and proven males. Out of the 79 crosses which had been made the progenies of 38 of them were subjected to the greenhouse downy mildew screening program.
The results of this screening is given in the section on
Only four progenies Imre obtained from the 18 in this group in 1957.
An attempt was made to duplisate this tee few crosses were obtained, and it will be necessary to continue this into next season.
Crosses 57122, 57150, 57157-and 57172 mere tested and 57122
(involving 2018) produced exceptionally good seedlings. Further information is given in the section on "seedling reaction of 1957 crosses to downy mildew."
Groups V and VI: open-. pollination of unproven and mien. females:
Out of the 87 openapollinated seed lots obtained in 1957, seedling progenies were obtained from 6h of them. A decision was made to retain or die., card most of this material on the basis of their seedling pf Ay performances
(mildew reaction and vigor). Following are the results of this test.
202 -3
2044
208.3
211-1
211-2
213.2
215-2
215 .3
222
301.1
301-2
301.3
302.1
302 -3
303-2
3064
304.2
306.1
306.2
306-3
308-3
309.1
309-2
1.114
1114
1134
1134
113-3
Uit.2
1143
115-2
122
2014
201.2
313-1
3134
316.1
316.2
405.1
4054
1405.3
406.1
406.2
406.3
407.2
;:63
..
..
14:94
..
..
1.28
2.28
..
....
--
--
...
--
...
6,58
9.54
0
3.
5.00
2.89
2.35
5.36
4.10
0.99
x.52
....
8.51
4.03
6.58
440
-5.812
0.31
2.50
5.57
5 059
57460
57061.
57061
57065
57075
57079
57085
57087
093
57095
57096
57097
57090
57099
57100
57101
57102
57103
57107
$7108
57109
57112
57113
57116
57124
57125
57139
57140
57158
57159
57163
57264
57165
57166
57167
"
No test
Discarded in 1957
Saved (Late
Discarded
N
Discarded 1957
Saved, used 1958
Discarded
N
Discarded 1957
Saved, used 1958
No test
Saved (Juggle.), no test
No test
Saved, used 1958 test
N w
Discarded 1957
Discarded
MO toot
N
N
N
N
Discarded 1957
N
N
N
1958
N
N
No test
Discarded
Saved, used 1958
of he of susceptibility were observed both among crosses and among within certain °roues. The use general testing procedures were follommi in
1958 as in previous tests cited above.
Pro
Seed from the 1957 crosses was germinated and seedlings planted by
S. N. Brooks. When seedlings were 10-12 nooks old they were heavily inoculated with downy mildew.
Inoeulma consisted of sporangia collected from several varieties and lines to include possible different races of downy mildew, although the existence of races has never been demonstrated tor suspensions of downy mildew spores were prepared by washing infected 'spikes" then filtering to remove the larger particles of soil and plant debris.
Inoculation was accomplished by spraying 20 ml.
of a uniform spore suspension on the undersides of the leaves of seedlings in each flat.
Humid conditions were maintained by covering the inoculated seedlings with cheesecloth saturated frequently with water,
Good infection was obtained and disease developsent was rapid and vere.
Seleotions for resistant plants were made with the assistance of
S. N. Brooks.
The number of seedlings from each cross was recorded.
A record was made of the number of ',atomically infeoted seedlings in each cross, and each cross was rated for mildew severity on a scale of 0 to 3 representing none, light, moderate and severe infection.
Selection of plants to be saved was based on both mildew resistance and seedling vigor and appearance.
table below shows tars deingr Widow,
26466
111
118 crosses
devsloP**4 t differsness eoptibility thin certain creamy The reaction of swains from the 1957 crosses to
7 severity prat
43-0P
44
45-0P
47
48-0?
4560P
50.0P
51
52.0?
54
55.01)
56-op
57-op
60.01)
61-0P
62
64.op
65-0P
68
6
7
75-or
77
79-0P
80 **
81 **
82 **
85.01)
87-op
93-op**
319
323
329
1%
210
219
108
98
85
317
322
208
319
312
107
109
108
215
105
321
104
107
319
320
325
326
312
108
319
322
305
314
7
1
0
1
0
4
2
1
11
3
23
13
9
21
15
8
7
0
11
9
1
1
2
1
3
6
0
10
2
0
11
3.41
2.18
0.0
5.24
4.11
0.92
1.02
3.53
7.2
4.
4.33
6.58
4.81
3.714
1.84
0.92
5.12
6.67
0.31
0
0.93
0.0
2.5
5.57
o.64
0.31
0.92
1.92
0.0
3.13
0.62
0.0
2
1,5
2
2
3
2.5
2.0
2.5
2.5
2
3
3
2
1.0
1.0
1
2
3
3
2
2
3
3
2.5
2.5-s
2
1
3.0
2.5
2.0
3
1
5
10
6
4
3
2
1
4
0
3
1
2
3
3
2
7
2
6
3
6
2
1
2
0
0
3
4
2
7
4
1
8
Based on a scale of 0 disease development.
**Denotes crosses am
2 moderate and produainr many vievor.",,.. as, well as
3 resistant seveme qpwilina-
292
/18
211
99
325'
206
212
303
319
.7,i
211
311
325
107
313
315
315
108
319
210
110
107
214
200
110
289
217
104
104
216
212
325
207.0?
208.0P
210.0P
221-01)
212.0P
2134P
215
216.0P
217-OP
218
219.0P
220.0P
222.0P
225
226.0P
228-OP
229.3P
230
232
233
238 e*
243
245
247
*IF
249
250
251
254
256
257
260.0P
mt.
5
2
5
2
2
6
2
0
3
13
7
3
3
15
1
2
0
2
1
0
0
8
0
2
3
6 o
0
0
0
2
6
8
2
6
4
7
7.27
0
0.93
0.50
0
0
0.92
0
1.91
0.93
2.83
2.46
lt:
2.28
1.42
0.96
4.62
0.93
0.96
1.90
0
0
0
0
062
546
1.91
2.20
1
1 . 3
0.68
1.57
0.95
2.02
1.85
0.97
0
26,166 581
2.22%
2714 a scale of 0 none, development.
light, 2 moderate and 3 - severe rosses producing many vigorous as mall as resistant seedling
2
1
4
1
1
7
6
0
2
3
2
4
1
2
3
1
3
3
5
0
1
3
0
2
2
1
1
2
1
4
2
2
2
3
1.5
1.5
2
2
2
3
1
2
1
1
1
3
2
2
2
1,5
2
2.5
2
1.5
1.5
3
2.0
1.5
2.5
1.5
1.5
1.5
1.5
25
2
2
2.5
1 .5
2 .5
2
2
Yield observation over 2 swims initiate that relatively molested as resistant to-greenhouse sereening twee severe field.
of the seedlings infested in the differous es field roe a
SPA ng pro standpoint resistance to systemic types.
Degree of systemic infection, re
118 crosses tested In 1958.
As parents ducting a high pereentage of suseeptible
degree of systemic infeetion should be reduced.
the three years 1956, 1957 and 1958 shows that this is
date for
1956
7,026
31,357
26,166
511
939
531
7.27
2.99
2.22
0 . 24.4
0 . 22.0
0 4 9.5
prosent agronomist holog10 on the basin of vi for
testing in the field.
274
and mildew resistance
Onthe basis of 3 years' results it appears oonolusive that green ening with downy mildew is olud natir nest
dewy ands, sereentni preps* roproaeeted Prollentiafat of transp3Aatod in tlos fiold under dal *posing whorls they
stet plants !dill be subsequently inereased for further evaluation
Selsoitions its were saved from 7 827
-plant nursery. All of these salections More female individuals.
Theey will be increased and grown in or
5-hill observation plots. Following is a last of them along with the accession numbers assigned and brief descriptive remarks:
Accession no.
Cross
56014 (X) Good vigor, large Bone, late, med. arose.
C 57001
C 57002
57003
P
5700k
C 57005
C 57006
C
57007
0 57006
G 57009
C 57010
C 57011
C 57G12
56053 (I)
56041
(OP)
56076 (Z)
56143 (OP)
56268 (OP)
56248
(OP)
56171 (or)
56205 (OP)
56211 (OP)
56234 (X)
56112 (OF)
Attractive appearancet lar bright cone, mad. aroma.
Large eons, shatter resistant, net. aroma, early.
GoOd gist of small heavy cones, early, med. aroma.
Heavy set of pals cones, mild,
Seedless, ;mill cones, bright lupulin, med. amnia.
Long sidearms, fairly rich, pleasant aroma,
Vigorous, good sidearms, tight cones, med. aromas
Large round cones hopped down well.
Not much vigor, firm cow:timed. aroma.
Good cone set, some shatter, high resin and oil.
Strong smooth vine, late-, small cones.
passible to obtain *deg** satin disease mereery provides ewe environmental *antral.
'WA for &rosy vdleiew infsotion her. An over mist spray erste* development despite unfavorable la automatic hneidistat is installed car an are where *boat 200 inii
Tits cm be tested im any one mason. The nursery area contains a spattering of mildow.sueceptiblo Tarts and maintenance of mildew.
The nursery area vas
1955. It is anticipated that ma avail for testing in 1959 or 1960
27
To eaialegme and dos ris for each male genotype.
To study phenotypie and genotypic correlations between pair.
of characters.
-UullaiJILEMatels
It is hoped that sufficient data will b obtained during the 1958 and 1959 seasons.
Reasons fo k: asters in o before a breeding pro efficiently. Evaluation of male breeding vales has largely been a matter of progeny testing which is time consuming and laborious.
Often times progeny testing has been incidental to the breeding program with the result that incomplete information is available, Methods are needed to evaluate maim; phenotypically, particularly for quality and yield, if possible, to aid in selecting breeding material.
Mature and extent of evious
The most valuable work along this line has been done in England.
Wormald (J. Agr. Res., 1915) presented a detailed comparison of the variation existing in American and English male hops for floral leaf and vine characteristics.
His paper was largely descriptive having limited data.
No attempt was made to separate genotypic from environmental variation.
Ehara (J. Fac.
Agr. Kya. Univ., 1956) made a detailed descriptive study of the comparative morphology of the hop.
Hama chi (Bel. Brew. Soil', 1955) published in Japanese describing development and differentiation of flowering branches. Most of his
se* dealt with the
*tenet poll eia she in I Pole of males sad hied wide differeness in Leith o pollee ng period. Farrar, News and Whites (Wye
44114 AM4
RIStof 190) tried to resin gland number in IOU hops with resin gontent of related females with no Suesess.
Work at Corvallis; anneal reports, Cie 7)
.demenstrated that mine exist in male fismamms in me
Recent work in Smgland has *hewn similar results but it indioation of the range that might be encountered in a group of satisfactory modification of a method determining a. A and /3 -Add in male flavor* has been developed by S. T. Likens, by which a relatively large number of plant* can be tested feasibly.
20 male lines were planted in a replicated trial of 5 rooliootione of single hill plots in 1956.
The five replications were made by vegetative propagation at the 20 lines and would be expected to be genetically alike in the year that they were planted. These lines were net selected for any particular characteristic, except that one was selected because of extreme earliness, and would be expected to represent a random sample of the male genotypes available at Corvallis.
17 different pedigrees are represented in the group.
Observations were made on the male lines for flowering behavior and branching pattern in the field.
Male flower samples were obtained by bagging the sidearms in parchment bags for 3-4 days while the flowers were shedding pollen. At the end of this time the flowers which had completed their pollen shedding and had abcissed were removed in the bags. They were then dried at low temperature in vacuum and frosen. These samples provided the material for
17
18
19
20
6
7 a
9
10
11
12
13
16
514.31)
X
)
224
317
319
322
320
323
424
X
X Early x Red Vine4
Unknown-5
X Belgian Burring x reggle43
X Late Clustar-5 x !Niggle",
X Tuggle x Tuggle-8
X Elsasser x Faggle-5
Brewers Favourite4
/ 19005 X Late Cluster...5
518
14254 C 51101 ti 308.2 (Fuggle..5 (51.31) x (Landlespfen-5 x (Golden
Cluster
Foggle-S) x (Landhopten-8 x (Golden Cluster x Tuggle...3)
521 G 1905! 11 Early Greta x linlaionn4
523
417
524
C 19060 X East Kent Gelding x Bavarian-5
C 19050 14 niggle x ruggle-3
C 19061 14 Late Grape x Fuggle.5
plant from whisk* samples wore availablo.
42Monimontal rot/alto
Partial results me g behavio floral development, eta Resin gland cants were mode On three replica s.
O replicate has been analysed for resin data and three more reps, will be analyzed during the coming months.
The datettoomoleted to date are given in the tollowin tables,
1 14* 4/
2 13* h7
16 4 7
14
$ 13
47
50
66
70
58
51
3
53
55
0
WWW
WOW
17
521
18
523
19
417
20
524
32
3 31
4
5
53
53
51
1933
* 49
7 58
$8
1 19 44
2 21* 49
3 23* h6
74
77
66
51
53
$1
414
39 70 44
516
44 67 53
51 70 55
96
96
94
96
93
9
94
72
93
78
3 27 49 105 55
416
47 72 51
5 18
41 100 47
1 17* 42
2 34 53
5 19
40
40
67
614
62
60 47
44
51
$8
4
44
76 100
70
6
63 ft --
97
3.36
2 24
3 3o
4 12
$ 21
49
49
$1
39
44
72
67
65
63
70
55
51
55
44
47
77
85
65
68
68
97
97
30
28
20
31
23
WM
Wift
WW
OW
..
..
..
..
...
..
..
..
.,-,
..
IOW
WW
WW
WW
25
28
23
25
28
2nd flower berm,
23
18
14
24
26
25
21
25
22
20
19 19 38 19 small plant replant 1958
-------÷
25
WW
56 22
31
ai; 23
24 49 25
..
1...
19
...
24
..
..
26
..
.
21 leaf flecking small plants leaf flecking leaf flecking
..
12
13 ma
25 small plant
32
..
12
21 small plant
27
23
-. Not obtained.
* Not verified by later observation, but probably corrects
No definite break between let and 2nd flowering(overlapping)i
1st and 2nd flowering added together.
106.a
6
223
227
125
221
7
8
324
224
10
31
11 322
12 320
323
11 424
15 518
16 425.1
17 521
18 523
19 417
20 524
Mean
ISD(5%)
04
1.0k
1.55
244 1.514
2.8$
0.91
.1.76
2.67
1
0,22
4.58
0.47
3.14
0.69
0.62
1.60
2.22
1.36
0.36
0.72
006011 0.3511 0.95
loll 3.08
620
0043
2.08
0.79
0.35
1.83
2.18
1.71
1.41
0.34
0.71
0.42
1.25
1.22
0.82
1.89
1.05
2.96
2.63
1.16
2.60
1.47
2.54
2.35
0.56
2.74
1.99
1.46
5.28
4.34
2.02
1.33
1.95
3.28
.
Data supplied by S. T. Likens
46.7
60.1
29.0
41.8
31.6
10
9.1
22.2
30.7
35.0
18.5
27.1
21.5
19.2
5506
164
29.
41.5
39.5
29.4
36.9
20.6
Nine
Nene
Small gland*
Big an isith few *lauds glands mostly an sepias.
lig flowers and anthers,
°Isar Asada.
small anthers, mill glands
Glands clear and light colored
Fism4 tight flowers
Glands small, nost2y on *opals
Anther famous deep, many glands ruptured.
Large anthers
Large, round glands
Small, light aolarod glands
Moue
Small, firm flavors, large clear glands.
Largo flower, clear glaccls
Anther* flat clear glands
Large anther glands vary in sloe
Name
Photographs showing resin glands on the male flowers
(about 11 x, scale is in millimeters).
Note resin glands on outer surfaces of sepals and in the dorsal furrows on the anthers; glands occur also in the disc from which the stamens arise.
RID
59
d in ear4 lines laths t inmost of the limes Olen ones, was a little surprising.
Heretofore, in the breeding have been eut down by August 1 or shortly after in order to eliminate unnanted pollinatianwhen the bags are removed from the female flowers. Here detailed data will be obtained on pollen shediting periods next year, although differences were apparent in 1958 from the limited observations made.
Significant differences were indicated for resin
The average numbers of glands per flower ranged from 10.5 in 106-$ to 60.1
in 523.
This is a six -fold difference.
Only one replication, of 0( - and r:' -acid a a but a few lines have two replications completed (not reported here).
In b.
general, fairly close agreement between replicates is evident at this time.
In the onezeplicate
-acid ranged from 0.22% to 2.514% * tenmfold difference. Differences of similar magnitude in -acid were also demonstrated. Differences in % total hop -acids (an arbitrary classification) were about eight-fold.
Gorrelation ooefficients were noUlcomputed between resin gland the various hop-acid components.
Scatter diagrams, however, indicated a rather loose positive correlation between gland number and
,
-avid a fairly good positive correlation between gland number
-acid, and a strong positive correlation between gland number and % total hop-acids.
This study will be dicouseed much more thoroughly next year when final and more complete data become available.
Deration of sxp,rimenti aired pre+
This trial is concluded with this summary of the results for 1958 s years.
The 29 entries were planted getative clonal material in plots replicated five times in a randomised block design.
The 27 experimental lines represented a group of selections which made in 1950 Out of crosses made in 1949.
Although most of them were intermediate in maturity there was considerable range for this charaeter tram medium early to late (later than in Late austere)
The trial has been grown under irrigation and has received Eras two to four irrigations per season.
Applications of 111, P, and E fertilisers have been made every year either by ringing the hills or by broadcast application.
Attempts have been made to control insect and disease pests.
The overall cultural program on this trial has been carried out in a manner consistent with good management practices.
Experimental results,
The following table s d data tram 1954 to 1958 data obtained in 1958 as well as information on a portion of these lines is given in the Corvallis-Prosser Yield Trial report and sections A, f 0Ro5-5 report.
3.3
28
27
2
21
22
26
5 103
1114 25,6
311-1 24.0
107.
26.0
139.
L.0
1
1284
26.1
130.1
108-1
124.1
24.1
122 -1
27.2
26.3
27.2
135-I 25.2
24.7
1234 23.8
224
23.8
1384 26.2
20 127 -I 22.1.
10
6
7
14
214
109-1
1044
132-1
23.7
2242
106-1 25,6
102.1
30.1
254
19
18
23
126-1 2793
125.1
28.1
1314 23.7
29
12
3
1
144-1 214.6
1174 24.6
1014 26.0
runlets 29.3
Mean 25.3
CT
LSD (5%)
3
1.8
a.
0
2270
2 1
5
12
3
2
3
0
4
2
7
1
2
0
2
3.
3.
1
1670
11.
3.630
7
2
10
1900 11
0
31
1310 w
3.820
1180
1910
1890
1880
1840
1760
201.0
1920
1750
1720
1570 1660
1000 1610
11490
950
1390
1590
1570
3570
3520 11420
610
1000
1510
11450
920
1200
11400
3.370
0
1860 U420
6 13
510 310
1
I/ Underlined yields greater than that of ruggless basis of LSD
After E. L. LeCier Mean Separation ARS.20.3, May 1957.
a
There were also in 1958 t at harvest time such differences are a reflection of since known early lines are usually drier at a common date than known late lines.
It is unfortunate that a satisfactory picking machine is not available at Corvallis with which each line in a trial could be harvested at its maturity rather than at the average maturity of all lines in the trial.
Such a machine would be of great value but it would be very difficult and costly to develop.
14 mean yield groups were separated in a five.year
rlr of o replications using BuncanIs YAM Is Range Test.
This analysis indicated that rather marked differences among the 29 lines were present during the five.
year study.
The highest yielding lines 112 -I, which, incidentally, is current ly undergoing advanced testing, produced significantly more hops than 23 of the
Fuggles the lowest yielder in the test, produced significantly less than all of the other lines except for 144-1 which have been selected for off - station testing,
The amount of annual replanting required has been recorded since
1955.
tate Clusters has required the most replanting during the period, however, 123-1 127 -I and 109 -I, have each required 10% or more replants each season.
Of the lines now undergoi.ng advanced testing in off-station trials in
31 t
p.25.
At Corvallis data were obtained acid and oil content and storage chareetoristies.
1
At Prosser data were obtained on yield and alpha -said oil content.
'field results are now available for 1957 and 195$ from thistrial and will, be summarized, Harvesting at Prosser was accomplished by an experimental mechanical picker,
The following tables present the most important information obtained on the lines in this trial.
Additional agronomic data can be found in the rimental Yield Trial under CRe5-1, Additional chemical data are included in Sections Ai I and C under CRe5-5 report.
Time did not permit statistical analyses of seem of the however differenoes among lines are obvious for several charaoterittics.
1.0s14
Denim
1034
104.1
1074
108 -I
109.1
112-I
123-I
124.1
127.1
lae.1
132.1
135-1
138-I
1394
144.1
17
10
20
14
10
7
214
10
}ban
(%)
LSD (5%)
16
12
5
1450
12140
760
1330
16
600
0
1500
1290
1130
1520
1370
1510
8
330
1540
1320
1630
870
820
960
1770
1370
1280
11
390
1450
810
1430
1920
1100
1320
1680
1170
500
1480
760
940
1170
1330
1280
9
310
1990
890
960
1890
1230
1050
1800
1070
380
1720
850
1190
1910
1160
1180
1740
1120
440
980
790
1470
1350
1280
NMI
9
9/10
9A7
9
I
Underlined yields exceed that of
* Taken from different trial at
Lets Clusiers
Corvallis.
Data supplied by C. N. Nelson
Data supplied by S. T. Likens
Based on remarks made by R. C.
(see continuation, next page)
Cadsby, Anheuser-Busch, Inc. (see continuation next page)
1
I
1274
128-1
132 -I
135 -I
1384
139 -I
14 4
5.88
3,80
7.99
Bunion
1034
1044
1074
108.1
1094
1124
7.71
3.08
3,06
4.14
4.48
5.01
14.94*
5.76
4.22
5.29
9.06*
3.14
1.54
4.74
3.27
3.41
4.23
ita
0.49
0.36
0.59
1.60
.
2.11
.60
0.78
1.47
1.46
3.34
2.89
3.26
1.17
0.83
0.49
1.30
0,80*
0,22
.
0.28
0.20
0.36
0.52
0,88
Orson appoaranoo; fair to good hop; bland but not off.
Strong; fair to good appearanos hoppy;botter than 128 -I &1074
Very good, bright color; oones fresh, clean; one of boot in group.
4.65
3.62
1.90
1.78
1.84
2.43
2.39
2.12
0.75
0.95
0.94
2.16*
0.21
0.85
0.6
0.47
0.61
0.43
0,31
0.31
0.18
0.31
0.49
0.29
0.38
rash, clean, similar
Slightly off - oily in aroma; good appearance
Slightly off (oxidised); good aparance; oily; better than
1pe444.
Fair to good appearancal bland, and strawy aroma; better than
135-1
* Taken from different trial at Corvallis
Data supplied by 3. T. Likens
Based on remarks made by R. O. Oadsby
44
Disealutien 00nOlnsiOn01
At Corvallis, si in the three year summary of yields, for sidearm 1 ture content at harvest time (not sheen in the summary table).
'fields in approximately 200 lbs. per more below the three year average in 1958, due primarily to an unusually warm season.
Surprisingly, Late Clusters did not de
40 W411, yielding only 860 lbs. per acre.
A moderate amount of downy mildew in 1957 is hardly enough to account for the low yield of this variety in 1958.
Seven of the lines outyieldsd Late Clusters in 1958, some of them utuggles" types.
Three of the varieties have outyieldsd Late Clusters over the three year period.
These are 1074, 112-1 and 123 -1.
At Prosser significant differences were indicated for both 1957 and 1958.
Late Clusters was the highest yielding variety each year, and on the basis of the LSD's exceeded the yields of 11 varieties in 1957 and all of the others in the trial in 1958.
These results indicate rather conclusively that observation plots should be established at Prosser from which to select lines for testing in that area. This could be done easily by planting the selections at Prosser which have been screened only for downy mildew reacti ©n at Corvallis*
It is apparent from these trials that considerably more relative vriability is present at Corvallis (CV of 16% in 1958).
There was room for only three replications to be planted at Corvallis in 1955, whereas five were planted at Prosser.
In future years, particularly for "advanced" yield tests less than five replications should not be considered.
Three replications may be sufficient for preliminary testing, however, if space is at a premium.
The alpha-acid and oil data are in line with last year's results.
Alphasiaoid and oil contents at Prosser were noticeably low for the second year.
seesidmring that the
Gorvallls when chemical data are to be
will have to be investigated drying temperatures could, at
Only a portion of the lines at Corvallis wars evaluated by ndastryreprementatives this year.
The lines evaluated were those been selected for off- station testing in Oregon and Washington along commertial varieties. Evaluation was nadir by different brew* master than in past years.
It was apparent that opinions of quality differ among individuals.
Mr. Frank Scheeigor liked 1154 and 11014 and didn't like
Late Clusters and 1284. Mr. R. C. Cadsby liked Late Clusters and 128-I and wasn't too highly impressed by 1354 and 1444.
Several of the samP were obviously immature.
This objection will be eliminated next year,
CRe5-2 (oAES 36) THE EVALUATION CT FUNGICIDES aINSIs
.
PMSAtue vezics
S.. 19% Anneal Report, p
7
INIXII cc tieId seals downy mildew control trials using three sprays mmercial hop yards of about 9 acres each.
Treatments consisted of three commsroial preparations of streptomycin (Agistrep,
Agrimyein 100 and Agrimyein 500) applied as sprays containing 1000 ppm streptewein
Two applications were made.
The first application was made
May 3 and 4 when new shoots arising from the pruned crowns were about 8 inches long.
The eeoond, was made May 26 and 27 about one week after the first vine training. Data on number of systemically infected shoots present were taken
May 17 and 19 and Juno 12 and 13, about two weeks after each application.
Plots consisted of 510 hills each and each treatment was replicated 4 times in each
Data on number of hills infected and, incidence s mic infection are given in the following table for the first applications on all three yards.
z
1
Agrimpin 100 2
3
4
1
2
4
3
2
3
7
6
5
S
14
4
2
3
2
3
1
2
1
6
II
4
6
5
5
3
3
9
11
6
8
7
8
5
8
11
13
6
16
It
7 lit
10
10
5
9
20
21
20
5
5
6
6
6
5
6
II
10
9
16
22
18
15
21
20
Cheek
1
2
3
4
43
49
55
47
17
31
22
34
46
73
55
73
28
23
23
31
61
52
78
68
64
75
66
81
150
174
188
188
Data were again sollectod after the 2nd application and awe shown in the next table' incidence of dowry mildew infested hills and sheets after seoond application of streptomyein
Agrietrep
1
Agrimyoin 100 2
3
4
A.grigrein 500
1
It
Check
-4
1
2
3
1
2
3
4
Yard 1
Number of inflated
!fats *We
8
7
10
6
12
16
17
11
5
5
5
4
12
7
9
6 lard
Number
2 of infested WSW)
4
6
8
11
14
13
5 12
Tart
3 number of infected
4
4
5
3
5
7
8
5
1
3
3
4
5
7
14
10
1
2
3
3
2
5
5
4
5
8
8
8
14
17
14
10
8
5
5
5
13
10
12
9
6
7
,
9
13
8
22 49
4
90 apilms reied by
31 61 before data vas o
41 65
Total
M ATT-V:Si
16
17
23
14
2 .1
37
38
28
19
20
22
17
7
10
11
11
36
36
39
27
19
19
18
20
a
*rowers mere yell satisfied with sad mot too coat27. There is still same quest
*eery. Trials will be conducted in 1959 to compa applications one and tee
On the basis of
A.R.S. has ruled that only st of be used on hops until additional data on retention of strop plants has been obtained.
A similar trial was conducted in the YakJme Valley of Washington by Dr. C. B. Skotland of the Prosser Experiment Station.
his data show 70 peroent reduction in spike incidence from a single application.
of 1000 ppm streptomycin spray applied when mew growth from the crowns was about 6 inches.
Later applications resulted in decreased control.
Tests mnducted by Dr. J. N. News of the University of California, Davis resulted in very little control under conditions of their experiments.
Time of application in relation to the developmental stages of systemically infected shoots appears to be an is portent factor determining degree of control.
red b obtained frog each
plots and combined to obtain expressed plant sap.
!.salts: of gap expressed from hop shoots treated llected at various intervals after treatment is shown in the table below.
A nazis= concentration ot 116 ifiri was reached 2I hours after treatment, by a gradual decline
72
96
2
3
5
6
14.3
3.7
5.2
5.6
4.7
14.5
3.5
146.0
25.14
23..9
10.8
2341
18 I
X2.8.
16.5
33.0
35.3
21.9
2814
288
3.2
336
)60
014
1.5
1.3
1.2
0.8
1.7
1.9
2.3
1.3
0.9
t tine intervals after the s raptonycin spray and assayed for stpad sap. Data fres those assay are givan in the table balm.
51
3.
3
14
6
8
ID
Ian Inhibitten sew widths
5.4
442 h42
3.2
2.5
ihreptionida osseantra-
,Pon (11114
10Pli
31.11
404
25.6
17.4
8.8
5.4
p
The pattern of absorption and tranelocation of streptomyoin by hops receiving a single spray of 1000 ppm showed a maximum absorption of 46 pia after 24 hours followed by gradual reduction over a period of 15 days.
to greenhouse tests conducted previously (1956 report p. 61) maximum absorption was the same, but field-grown hops retained detectible quantities of streptomyein fora much longer period.
Following a second application of 1000 ppm stroptmaysin 21 days after the first application, the maximum level of streptomycin absorbed was much higher than after a single application on either field or greenhouse grown plants.
This effect oould be due to age of shoots treated, effect of environment on absorption, or afflict of a previous application of streptomycin.
Streptomycin irms found to be readily absorbed by young hop shoots d in measurable quantities for up to 16 days.
of treatments. During the 1957 season dowry lading
Mss unusnallya re axx was not eontrelled is this plot.
The following season
(1950 hill dieamout was very extensive.
salts* data table below shows out among the treatments.
Effect of Cheoal Soil T of Hop Crowns, (Treatments taken in 1958)
Treatment
Chloropicrin
Papas
Wiens
Terraclor
Check
4
0
1
Member of 10 hills doad
I
5
2
3
2
3 4
2 0 2
5 2 2
3
2
2
2
2
En
1956, data.
Total dead
12
7
11
6
12
Percent deems
304
1,5.4
30.0
40saaienand,Conalusionss
Root die -out was *over* in the spring of 1958
Winter injury was not a factor as the 'inter of 1957.1958 was the mildest in ?seen% years.
Severe root die...out followed a severe attack of downy mildew.
These results support the observations of many growers that replanting is heavy following a severe downy mildew attack.
The summary data table above shows that die.
out was as severe in some treatments as in check plots. whetter Terracior or
Yapam reduesd die-out statistically has not yet been established.
h.
eeeporation
1910.
These tie% nee number of vines per plant fort and preaing, time of hoeing, and the use of various cover crops. Many of these studies were of a preliminary nature and more complete information is needed.
Sines there are interrelationships between field practise and quality or between field practice and disease factors, this lino project necessarily entails the work of specialists in various fields. For that reason much of the work reported in this section has been obtained by cooperative effort of more than one investigator.
In 1958 the following studies were conducted under this line projects
1.
Chemical Stripping and Suckering Trial.
2.
Irrigation.Fertility Experiment.
Effects of Gibberellic Acid on Puggiea.
3.
4.
Date and Severity of Pruning
Trial.
5.
Cultural Studies With Hallertau Hope
VOSS
Duration of experiments
To be discontinued in 1959
sin 190 Annual Deport Ps.
semi of the plots mere he season.
8610 1957 &M al
TUN data obtained in 1957 fish a n of $ residual re of treatment.
They are given in the following table.
The effects of two years of stripping and suckering on the yield of Tuggle.
in the third year (all plots hand stripped and suckered in 1958.
Treatment and rats in
1956 and 1957 wt. (lbs./ DUD
1
Percent difference
Check (hand worked)
38.3
21,2 16
Cheek (not stripped or suckered) 143.0
31.6
27
Endothal at 3# / 100 gal. water 32.1
22.1
31
Endothal at 5# / 100 gal. meter 36.6
22.1
Endothal at 7# / 100 gal, water 36.0
21.5
39
60 an
CV (S)
LSD (5%)
37.2
1,
6.7
23.7
7
5.1
Discussion as d cone ions s
The results obtained in 1958 residual effect of chemical stripping and suckering on yield
plots and the handoworked shriek 41000 there indisated among all yids. Seeendlys the fer the am-stripped and suckered plots were mesh talents* The better the job of stripping and
7* r was the rednetion in yield irk 1958.
It be renenbered at thin point that yields in general in 1958 were mach lower than in the previous two years.
There was only a 27% &moose in yield whore basal growth was allowed to remain in 1957 as compared to a demos's* of 31-60% where basal growth was moved periodically in 1957.
Although those results indicated that complete stripping suckering have pronounced detrimental effects on vigor the following year, it is felt that this operation should still be carried out early in the season to avoid or control 80/92e disease and insect infestations.
This study points out that the translocation of food materials from the basal growth to the roots in the fall should be studied more thoroughly.
See 1957 Annual Report, page
T1 1957 Annual Report, Page 68, plot laysout, treatments, and goner
Tree s a detailed deseription of
B I
II
III
IV
The irrigation scheduling in 195
Inches of water applied to treatment
719 cation on each date in 1958
6 7,131 82 86 ME_
Total
454
1.12
1.16
1.07
Treat. G
II
III
IV
Treat. 1)
1/
17
I
Treat. B I
II
III
17
2.26
1.4
2.62
2.45
2.60
2.71
3.84
3.
3.56
2.36
3.12
2.94
2.42
2.83.
3.34
3.79
3.39
3.04
3.52
3.17 24.03
2.70 11.078
2.84 13.06
3.49 16.81
11.42
6.35
13.19
Notes Irrigations made on 7/5 and 7/9 mere timed to deliver an amount of water calculated to bring the upper two feet of soil, to field capacity.
Those made on 7/16 and later were timed to deliver an additional 4,46 inches of water beyond that required to bring the upper two feet of soil to field capacity.
kn.
0
CT 10%j I
USD (5%, all)
(5%, moisture 1
230.
Underlinsd yields are significantly lover than check, basis lass.
A. NO
Oat& moisture
EsMed.moisturo
Avg.
a
3%
Alpha-acid pores
3.61
3.41
3.21
3.41
3.68
3.58
2.96
3.!
3.36
3.02
3.41
3.26
A.No irrig.
0.55
D.High moiste 0.42
E.Medmoiaturi
0.44
Avg.
0.ta
CV 7%
Oil content (mils4 3.00 gm.
0.64
0.17
0.51
0.54
0.64
0.48
0.54
0.55
0.60
0.51
0.57
0.56
0.61
0.47
0.51
0.53
hope men of bepe in the sown& low. The rosette reatmemts Ineept all intention trea more than the ohs*. The main responae to f however there was ems response to potassium.
debit* response single irrigation early yielded onsiderahly a nitrogen response
Irrigation treatments affected the alpha.aeid content art the b0141 irrigation treatment lowered alpha -said percentage The appli a onbination of nitrogen and phospborns increased the alphapaoid pe
Oil content was decreased by irrigation treatment and increased by application of nitrogen fertiliwar.
There were a Wombat eef s durimg an irrigation on some of the plots when water from the perforated pipes failed to reach out and sometimes two of the gypsum stakes with the result that in scud cases the moisture tension records were not as reliable as they might have been.
Attempts will be made next year to overcome this difficulty.
fa bare 1444/4 applied stage. of growth serial:4s study in 1958 was preliminary.
work will be carxied out for another one or.tmo
years.
Oe for rtaJOLAR Chit works
At tlee present time, this has only academia interest.
Since gibbereilic acid has such pronounced effects on other species of plants was decided to try it on hops.
Nature and extent of POOViOna work:
None on hops.
Good accounts of this material and its use on other plants are given in ARS Special Report,
ARS 22-47, 1958 and a technical bulletin,
"fibre", published by Merck and Go. Inc. of New jersey.
Procedure
A trial was laid out with four replications of 1 a 5 hill plots, with n a randomised block design.
Two concentrations of gibbersllic acid in water solution were applied at three different stages ©f growth.
Each ion was applied when the hope wore about five feet high, when they were about 10 feet high, and when they were just coming into flower.
Each plot received a treatment only once.
The spray treatments were applied at low pressure (5-7 psi) in order to minimise drift.
The first treatment was applied on June 11, at which time the hop vines and leaves were wetted until the spray dripped from most of the vegetation.
The later two applications on June 19 and July 2$ were not as thorough, and spraying was done only until most of the vegetation had received some of the spray.
no ow 11.6 fi hig10
503 Pia
100 pin
500 PP=
100 ppm
930 PM
Maas cl (%)
00 (5%)
28.7
240
20.h
18.9
Zh.4
12
TA
7/6
7/16
9.0
94.9
10.7
10.2
TA
7/2
8.8
7/5
6
1.6
h
3
8
13
11
24
10
14
12
N3 ha)
4.22
449
3.60
2.69
MT
0.17
ante had produced burrs
62
63
Fairly wide ranges apparent* however.
There wore to differenoel in length
The most striking aspects of this study were the decrease in cons die delay in flowering time caused by the earliest application of the acid* particularly the 500 ppm rate.
Following treatment on June 11 there was considerable succulent spindly growth apparent a few days later.
By
June 25, hot weather had blasted most of the sidearm tips and the flower buds which had just formed.
Later there was a profuse amount of vegetation, particularly high-up on the plants.
it is believed that much of the flower development on this treatment resulted from a esscend" blooming which accounts for the delay.
It appeared that most of the inflorescences developed into cones causing a much higher count to be present than on the check plots* but these wore greatly reduced in size at maturtV
It is interesting to note that late treatment with gibb acid caused a reduction in alpha -acid content.
As a general rule alpha-acid content is not influenced too greatly by treatments which affect the vigor of the plants, however, such results apply to material whose action is of a different nature.
There were no apparent differences in maturity, supported by the fact that the hops did not differ in moisture content at harvest time.
As a general rule, moisture content of the hops is closely associated with maturity.. within a given season for a particular variety.
Rei4111011.1 for widertak
See 1956 Annual Reports page 104.
See 1956 Annual Report. page
Restates
Harvest weights for 1958 and the 1956 to 1958 tie year flowering date, sidearm length and Alpha-acid content are given in the following
Data obtained in the Tine and Severity of Pruning Trial in 1958
Treatment
Harvest wt.
Harvest wt.
(lbs.hlet)
Date initial burr
.
1958
Sidearm
124
(inches longth
)
M
22 13
33.9
6/24
% alpha.
1958
4.15
A no pruning 24.0
20
B 4ed. 4/11 23.1
33.9
6124 9 4.16
3,52 c Ser. 4/11
22.7
21.7
34.6
6/24 18 8
D Mo4. 4/18 36.6
6/24
23.
11 3.90
25
E Mo4. 4/25 24.9
35.4
6/28 7 3.96
LSD(5%)
23.3
6
NS
34.9
4
NS
..
..
NS
21
4 *
2 *
10
11
3
3.94
--
* Incorrectly given in 1957 report
Differences in sideara length hare been
repox ,axed petition will not be mad* this year year s masa should be expanded to include more than one variety and cover a elder range of dates.
This will be done as soon as possible.
3.'1957 ul
The funds to support this s of studies were not received from the brewing industry in tins to get t relax system established f ©r the
195e season. The trellis WA4 completed during the summer, and some results will be- forthcoming in 1959.
A.
raftxaticn of Strobilss and Flowere, lbw% pigs 100.
(ass 1956 Annual
The Obialcal NViluation
Pew 108).
of Leaves, ( 1956 Annul Report,
Investigations Of Analytical Methods,
Report, page 100.
Strobilas Al
1956
Annual pure ions
Se. 1956 Annual Report, page 109.
See 1956 Annual Report, page 109.
See 1956 Annual Report, page 109.
from the Quality Trial was taken as the individual st9es matured.
All replications were picked at the same time for any particular variety.
Samples from Corvallis.Prosser
were also taken according to maturity, however, replications of a variety may have been picked as much as a week apart.
sperimintal Results*
Alpha --ate determinations were made on 7 experiments involving 2 line projects.
Detailed discussion will be found with those projects.
Results of
NL.acid analysis on advanced experimental varieties appear in the following three year summary table.
higher oCueseist valises than
1354 has been lowest in Alpha
Varieties in the Corvallis T set in 0( eusid produstien.
1957 was the first year for the
(LewieBreen Corvallis) and In 1958 only 10 varieties wore
At the a time of which appear in off - station trials, except Breuer's Geldj.
the
Pressers Varieties fran Prosier, have been very inconsistent during years.' The 1958 were very smell and dry.
The 'Apulia granules were often orange-ibrown, indicating the possibility of over-drying, which may account for the unusually low
06.acid values,
Sacrament's Since 1958 was the first year for Saeranen r,, the hops were babies.
Their appearance was good and °C - acids were normal to high for the respective varieties.
There are two anomalies in the California samples:
1.
135 -I, normally very low, showed 5.69% ,=:,-( -acid,
2.
128-I, normally very high, showed 5.26VC-sicid
Alpha acid analyses have been made on samples from the following
Agronomic Trials (CRe54): 1.
Pruning, 2.
Gibberellic acid, 3.
Irrigation -
Fertility All were the variety Fngg].ea.
Pruning. For the years 1956, 1957 and 1958, no differences in meed content have been indicated for any of the pruning treatments.
Irrigation- Fertilitys Samples were analysed from 4 reps. of law, medium and high moisture plots, each containing Neill, N2Pill, N2P0K1 and
Nei% fertility plots. Differences in (DC -acid content were indicated
(1% level) for the 3 moisture levels, but not for fertility treatments.
-aol4 von
69
L.O.
Fogg.
B.G.
Bal.
Back*
5.e8
4.06
5
5.33
5.88
3.80
8.55
8.11
7.99
5.13
7.60
741
103.1
104.1
107.1
108.1
109.1
112-a
123-1
124.1
127.1
1281
129-I
132 -I
135.1
1381
139 -I
144.1
3.96
3.34
3.08
411
3.47
3.06
4.63
3.97
4.14
4.56
4.28
4.48
5,30 6.30
5.01
4.94*
6.94
6.33
5.76
4.39
3.98
4.22
5.32
5.72
5.29
9.82* 9.70* 9.06*
4.45
3.51
.3.14
2,66 2.38
1.54
5.22
5.47
4.74
4.14
3.78
3.27
4.78
4.90
3.41
* not included in Quality Trial
.45
5.20
8.03
9.43
8.04
647 5.63
5.92
3.41
2.20
5.61
4.24
8.56
7.09
6.93
7.05
8.92
6.24
6.82
6.28
10.30
6,25
3.10
2.46
2.85
2.63
3.54
4.27
5.94
3.52
,plaes from
5.21
6.
4.23
6.75
2.41
2.47
1.60
1.98
2.04
1.47
3.44
3.99
1:46
4.50
5.22
3.34
2.51
4.31
2.89
3.91
5.95
3.26
5.19
6.70
4.65
4.40
2.90
3.62
5.15
3.54
1.90
2496
5.00
5.70
6.05
5.26
3900 1.78
2.38
2.45
4.32
4.90
1.84
2,43
2.69
3.63
2.39
2.58
2.35
2.12
5.69
3.88
4.35
Yield Trial and Breeding Block
Voris
L.C.
Tugs, aa,
Bull.
103.1
104.1
107.1
loa-I
109.1
112.1(2)
123.1
124.1
127.1
132.1
135 -I
138.1
139-1
144.1
128.1(2)
% d
26.4
27.8
31.7
29.5
25,0
26.8
25.7
26.8
26.8
27.8
324
29.6
28.3
22.8
27.8
27.2
29.3
22.4
Orion oil
Color. 8POotr. Gtair. C.0.b.
.73
.99
2.89
2.40
1.50
1.62
1.14
.514
1.03
1.11
.80
1.06
1.17
.30
1.66
.86
.34
.81
2.84
5.9
3.8
8.0
7.7
3.1
3.1
44
4.5
5.0
4.9
5.8
4.2
5.3
3.1
1.5
4.7
3,3
3.4
9.1
7.4
4.7.
10.2
9.5
2.1
1.7
4.4
4.7
6.1
6.3
5.8
4.3
5.6
2.9
1.6
5.7
4.3
2.8
8.9
5.2
3.3
6.4
6.2
1.2
1.4
3.2
3.2
3.7
4.3
14.2
3.3
3.5
1.8
1.0
3.5
2.9
2.1
6.8
5.5
3.7
9.2
8.2
.3
.4
3.9
3.6
4.2
5.9
4,0
3.1
3.4
2.5
.6
2.0
2.7
1.0
3,8
2.0
3.7
4.2
6.6
5.5
2.6
3*5
3.2
2.0
2.2-
2.7
3.2
2,3
3.2
3.8
3.3
3.7
3.2
394
26.0
35.4
39.1
23.4
28.6
24.5
40.4
304
17.6
37.9
38.1
46.4
22.1
148.8
23.3
39.0
23.8
30.6
(1) These values are averages of the individual analysis of the same replications but different samples.
(2) These 2 varieties not from Quality Trial but are from Rep.5 of
Trial,
Yield
72 p.111
Reason for
See 1956 Annual Report, p.
extent of pronto works
See 1956 Annual Report, p. ill
See 1956 Annual Report, p
1.39Ortaente.1 result's
Oil yield meaeurements ware can 4 replicatit levels containing 4 fertility treatments each. Results and discussion will be found under GMe5,4, Fuggles
Irrigation-Fertility experiment.
Oil, yield measurements on those varieties in advance tests have been completed for the third year from the Quality Trial at the East Farm at Corvallis.
The second year's data are complete for those varieties at the
Levisoarow Seedless Yard at Corvallis and for the material at Prowler
Washington.
The first year's data on the material at 6acramento,
Calif. are eomplete.
With fee exceptions, the oil yields for each variety in the Quality
Trial are fairly constant over the three year period.
The same is true for those varieties in the Seedless
Yard.
edless la-la seeded hcmes
However oil yields s sr to be higher
Samples free Prosser were found to be very low in both axd oil this year.
-acid
The cones were small and the lupulin was often dark
by hops and may net be typical. Ths oondition.
of oils 1.
7 Quality Trial by gee
the oon thin 4 varlet n of each weapons,* measured instrument with which these antiquated and inadaqu consequently the results lack a great deal in precision.
in spite of these handicaps, the chromatograms obtained appear to be fairly specific for each variety.
7
2.89
2.60
2.48
2.11
Buda
103.1
104.1
1071
108.1
109-1
1124
123-1
124-1
127 -I
128.1
129-I
132-1
135-1
138-1
139 -I
144-1
.78
1.17
.88
.04
.70
.83
.49
1.43
1.13
.92
.72
.75
.60
.84
.95
1.34
1.25
.94
2.13
1.30
.80
.52
.75
.48
1.20
.21
.85
.76
.57
.69
.64
.60
.47
1.50
.66
.61
2.08
2.44
1
.79
1.40
1.85
.88
1.59
1.17
1.32
1.03
1.47
.92
1.44
1.42
.31
1.43
.60
1.28
.95
.53
.69
.75
MM
Mellt
MM
GeM
WM
.55
.49
.76
.22
.28
.20
1.22
.36
1.34
.52
1.06
.88
.92
.53
.43
.31
.79
.31
.31
.60
.90
.144
.58
10115
.18
.31
.49
.29
.38
41ml
--
.149
.28
.40
(1)
(2)
(3)
(4)
Each value is the average of data from 4 replioatio
Sines determinatiow
1 replication only.
Each value is the average of data from 3 replications, see appendix.
Single determination -- 1 replication only.
.51
WM
*NW
SPW
OMP
.53
.41
.50
48
Chressie
(VP Crop drY Separated an 10 toot
Lite.
2
3
3.
2
3
28
26
10
7
5
8
7
10
15 a.G.
5
8
8
10
3
6
6
3 li
8
6
7
6
6
7
103-I
135.4
2
3
1
1
1
2
3
4
1
2
3
36
144
55
50
66
58
59
50
53
50
145
30
1 'gyros=
2 Mathrimmosylkatime
3 aphaerearyaphyllese
5
Unknown component present in most varieties
8
5
It
6
5
9
8
8
5
6
6
6
6
6
7
23
18
19
17
15
1
17
6
6
10
3
2 it
Rave additional,
peaks neck M.N.K.
High *r.
0
7
7
9
This reperamoved from trial,
tions
This work is being done in cooperati
Agricultural Chemist
Oresen State Co
Lwerlmentalliesultet writh the De
1957 Crap he past, samples were taken from
Trial est Farm.
One sample of about 5 lbs. green, was taken from each replication of each variety.
After drying at 118°F.
in a cabinet draft drier,
4 sub samples of each were prepared, one of which was analysed immediately for
...acid and oil content.
The remainder were put into 38'F. storage for analysis in December, March and July.
Data from these tests are summarised in the following table.
Detailed data will be found in the appendix.
During 10 months storage at 38°F. there was an average loss of only 7% of the (k'` -acid originally present. This compares with an average less of 27% in 1956-57 Oil losses this year averaged 20% compared with 25% last year.
(h6% and 39%)
Bullion and Brewer's
Told sustained very little
-acid loss this year, (5% and 3% respectively) but suffered heavy oil losses,
128.1, the rich experimental varied* held up very well for the
sit oC 444,41 tor 441
t ay.
this Tsar.
ifiAmelts of Storage 2044
0.S.C. Bop lards 190 erip
Sri %, BiV1, Oolorinatria asidesis et is in an reps involved A s even dry hops vitas la
Variolif
1 Lets
Clusters
5.4143) 5
( )
0.48(31 0.47(3)
2 Tunics
3
5.34(4) 5.39(3)
0.75(4) 0.72(3)
6.04(3) 793p3
2.89(3) 2.25 3
4 Bullion a-acid oil
7.60(2) 7.76(2)
2.14(2) 1.32(2)
5.25(4) 5.39(4)
0.85(4) 0.67(4)
7 «801
7.52(3
2.320
1.76(3
7.26(2) 7,20(2)
1.90(2)
5 103.1
a-acid oil
3.29(4) 3.19(4)
1.38(14) 1.03(4)
6 1044 a-acid oil
3.47(4) 3.68(4)
1.48(4) 1.19(4)
5.36(3) 5.140(3)
0.39(3) 0.39(3)
3.00(4) 2.88(4)
1.15(4) 0.93(4)
3.26(4) 3.39(4)
141(3) 1.10(4)
7 107 -I a-acid oil
3.97(4) 4.16(4)
1.04(4) 0.91(4)
8 Ima a-acid oil
628(4) 610(4)
0.70(4) 0.67(4)
3.85(4) 3.87(4)
0.86(4) 0.86(4)
3.88(4) 3.70
0.60(4) 0.6
9 109.1
a-avid oil
6.30(4) 5.40(4)
1.13(4) 1.11(4)
4.99(4) 4.72(4)
1,29(4) 1.06(4)
10112 -1 a-acid oil
5.35(3) 4.77(2)
1.00(3) 0.86(2)
4.23(2) 4,88(3)
1.00(3) 040(3)
11 123-I
22 124.1
a-acid oil
6.32(4) 6.57(4)
0.72(4) 0.59(4)
6.47(4) 6.214(4)
0.55(4) 042(4) a-acid
3.98(4) 4.39(4)
0.84(4) 0.75(4)
3.63(4) 3, (4)
0.75(3) 0
13127 -I
14 1321
15135 -I
16138 -I a-acid oil
5.83(3) 5.52(3)
1.25(3) 1.03(3)
5.13(2) 5.42(3)
1.08(2)
1.16(3)
1to o44.il
0.27(2) 03373((43) a-acid oil
2.38(14) 2.40(4)
1.20(14)
0.96(4)
2.13(4)
2.07(14)
0,98(4) 1.00(4) a-acid oil
5.45(4) 5.74(4)
0.69(4) 0,68(4)
5.48(3) 5.56(4)
0.50(3) 0.36(3)
12
13
2
13
14
7
0
148
10
39
46
33
25
17
10
6
10
0
19
26
17
44TAIRA
Art x-6cr
OT
(1)94n
(c)09'0
Pro nog-,
TTO
(tvolen
(1094c
10991 sex
'c
$S`0
(
(n voun
Mom
MIL
Mg
Cream, rio
COMM'
'VI
(I)
Mt=
A a paipulme ;Asp
I I0
C/3
(T)001Z
(1)WE
9' (T) rt tit
Oa
9'9 ra-P0
*9
LIP
See 1956 Report* p. 115
Nespons for undertaking this work:
See 1956 Annual Report* p.
Natt of preview: smirk
See 1956 Annual Report, P.
sults during the am r a 5 replications of 2Q male lines were collected
of 1958 as follows *
When a flowering branch began to shed pollen parchment bag 1411 placed over it and left for 3-4 days* shaken to break antbars etc. loose, and taken to the laboratory where tt was dried under vacuum at 40-50°0.
After all samples had been collected; material from each replication was composited to give a single sample from each replication or a total of 100 samples for the entire trial.
These were held at 0°F. until analysis.
Chemical Analyeii.
FaTerienee with counter current distribution during 1956 and 1957 indicated that a substantial loss of 0/ and 73 acids occurred during analysis, (20.60% loss).
Ion exchan resins stigated as a means of separating acids from residue material; (see "Investigation into Chemical
Methods").
This was found to be satisfactory and analysis for and acids of at least 2 replications of the 20 varieties is well underway method in present use is outlined as follows:
The
with sufficient methane/. to give a Alemeentmatien of *beet 200 rs per *1.
gxtreetien it
5 minutes Ina blender
(Serval sdhsequent oentriteging at about 1500 r*p.lmo
The superna into a polyethylene bottle and maintained at
OPT exempt withdrawn for analysis.
Two aliquotes of 0.5 and
2 inlet are taken for *Wyatt'
One is diluted and read directly at 332 mu on a Begawan Nadel B spectrophotometer and the absorbance recorded.
The ssooud is placed onto a 25 am (9 am appears formate saturated with to be sufficient) x 1 am column of Demexmil x methanol. With the flow adjusted to 0.5 to 0.8 mien per minute, elution, of the c( and/3 acids is carried out by addition of
15 els. methanol followed by (2) 60 mis. 0.3% formic acid in methanol follownd by
(3) 100 mls. 2.5% formic acid in methanol.
5 ml. fractions are collected, made basic with
6N NeOR and diluted to 10 mls methanol.
These are read at 332 mu on the Nadel *B" and a graph of absorbance vs. fraction no. is coostructsd.
acids.
From the graph is determined which tubes carried the
(:-Nc acids present
Absorbancee of these tubes are added and the amount of is calculated. Total absorbanoe collected is compared with the original absorbance to determine approximately the amount of absorbing material which was lost during the analysis.
Experiments have shown that lost absorbing material is neithern; nor cid
Detailed listing of analytical results will be found with
GB05-1.
In general 0C-acid has been found to range from 0.2% to
2.5% while
In most acid ranges from 0.3% to 3.1% for the 20 varieties analysed.
Ca Ms -acid exceeds
-acid, however there is no consistent ratio Some varieties exhibit higher than acid.
foreign aboorbors'i
< .
cVsoid
10 15 20 25
Fraction number
30
Fig.
Typical chart showing the separation of
0-and,-,5 acid from male flower extracts on ion exchange column
p.
at of the
1 likely associated with moisture
"fs. nose* is undoubtedly * result of the of I
It is the object of this SU
11114 chemical changes 'which accompany maturity to evaluate some of the physi order that better descrip of vs turation will be available.
ject will continue until 3 seasons, data are accumulated* loosens for undertaking, to extend the information available regarding physical and chemical hangs which accompany the maturation of hop strobiles*
"tyre and extent of previous works
Much data has been obtained regarding the accumulation of ,x-iwacidvand
Atoll during ripening* Some work on the relation of moisture and oil of hop strobiles to maturation is available.
It has been reported that the *starch content of hop cones decreases with ageP
Other crops such as corn and peas depend on moisture content and voluble sugar content as indices of maturation.
Samples will be taken at intervals throughout the a on from at least
afeNl lid ANS of pus of cK- assi
-acid content* hop a criteria for maturity.
will be used for the detersinatiee of
A. third portion sd.11 be dried and used nt and dry matter per sone will
2 as quality estimates and the stilettos.)
'mortise which ars be related to these three evaluate their rlationabip with maturity
XS
?ire samples of Late
Clusters
19 57 and held in frozen storage st 14 to September
These were ussd for preliminary work to establish procedures and provide a basis for planning sample collections during 1958.
It was felt that the plant juices in the hop cones would provide easily obtainable test speoinan and at the same time be very likely to respond to maturity changes. At the same time it was expected that the amt of plant Jules present would be a function of maturity since the total moisture present drops with aging.
Procedure for testing 1957 sample's'
The frozen sample was thawed and weighed.
It was pressed in a
Carver hydraulic press and the first 5 ills of juice to appear was collected.
The pressure was raised to 1000 posoi. and all juice that was freed was collected.
The pressure vas then increased to 6000 posoi and the remainder of the juice was collected.
Nearly all the expressable moisture was obtained at 1000 posit The pressed pad was weighed and the expressable moisture calculated.
Samples of the expressed were evaluated for refractive index,
Pick PfV00 date eater
Turbi
CVO, misty 2
1114
8/19 59.2
1.3390
8/23 55.1
1.3394
8/27
6%5
51.8
10415
1.3398
1.331401
1.31412
1.3406
1.3399
1.3398
1..3413
.623
4.19
649
5.11
xs x8
31
53
9/12 43.8
1.34214
1.31428
1.31438
6.75
( )
(2) estimated that 95% of the juice was collected at 1000 p.s.i.
Using #54 filter.
155
All properties measured mere influenced by maturation.
As expo of refraction, % soluble solids and turbidity responded nearly the maturity since they all measure similar properties.
Although total moistu content for this series is not available it could have been expected to have dropped from around 80% to about 70% during this period.
Expressable > isturs was found to vary over a much older range, suggesting that it might lend itself will to a maturity index.
55 a
Inamosaabla at 6000 Neste
8
It 9/3 013 awataal oat abentaALobwite
29i7
4 9/3 its
The 115. Mlituritr Og$
(Iv lair Ruch ) t riggloo from on the CoU.Rwr Form whioh Fromm*** on opporttmity to adopt toot prosody:roe to trash kepi.
a g
The foil
9100 n was evolved f
0100 A.M. on Sunny da
These were picked in a few minutes after picking thylene bags and me
These were returned cloth bag for juice
350 g)* rial runs, Samples were ximately 5 days apart, into four packages within the laboratory and processed as quickly as possible*
1. 6004000 grams (Into dryer) Total moisture content and dry matter and acids.
600 germs
(Distill) Oil yield grams (Freese) Cons oount Expressible moisture grams (Grind fresh and press out juice sample) Reducing sugars,
Index of refraction, Turbidity, Soluble solids
and a held the is, A second sample was is the uted with SO ethanol for sa ve analysis laboratory and
1 al ali
At the second collection a samples was ground in the field and a sugar sample taxon. Again a second ample was grand at the laboratory and prepared for sugar analysis.
Results of these tests are shown in tablet. From the results it was concluded that samples could be transported to the laboratory
Table 2.
The effect of time after picking on concentration of reducing sugars in hop cone juices. # Ong glucose/m1 hop oons Ni4O av of Licata determinations
Date
W11111110.
7/17
Sample No.
1
Pressed i 600 p.a.i. in field
22.9
Ground in field
IMMO
Ground in laboratory
22,6
2 29.2
4140 26.8
7/22 1
2
16.6
15.5
15.9
16.1
* Samples taken in the field were about 20 minutes after picking, ground in lab. were about 2 hours after picking. Variety Fuggles.
Method of Somogyii, J.B.C. vol. 160, p. 61, 1945.
Samples for processing without fear of altered reducing sugar values.
From juice samples taken in this manner, aliquotes were withdrawn for soluble solids index of refraction and turbidity measurements.
Results of the preliminary trial are given in Table 3
80.7
54.7
53.3
5144
PA
50.3
(Spootro.) % 6.2
6.0
5.2
5.3
5.7
5.8
6.0
Oil Yield (Omen hops)
(a1s/100 g, dor.b.)
0.78
0148
3.7
3.8
2.9
2.8
0.99
0.37
007
2.9
2.8
1.32
1.23
2.1
Reducing Sugar
(ag glimpse/ at juice )
23
27
16
16
114
16
19
Soluble solids
5.7
6.5
6.5
6.3
6.14
6.14
6.2
6.5
6.8
(iCIett
155 5 268 394 200 720 2650
18
11411
406
Index of refracti ©n
1.31418
1.31
1
1.31410
1.31418
1.34
1.3406
1.3 17 1.3423
1.3402
Cone weight
( mg d.m. / cone)
231 268 283
0
710.
at the beginning s were just lowing Tables out of burrs Results of these tests are and Cr aphis.
Save we actumulated in a different r for Yuggles than for
Bullion and Late Clusters.
In Fuggles the 0C-said was laid down at about the same rate as dry matter from the outset and held up until 4 weeks miLt the normil harvest date.
In Bullion, cc -acid accumeated faster than dry matter until about midsseason4 then appeared to fall off steadily.
Late Clusters Showed a steady Louses* its %
-acid until a few days after the normal harvest date, and began to decline after that. When calculated to milligrams
-acid per cone betas approximately the same picture is presented.
2.
scids appeared to follow dry matter for all three varieties for the entire season.
Ito loss of
-acid accompanied the build-up of
-acid in contrast to earlier beliefs.
3.
Dry matter content increased steadily throughout the season.
Since the overage dry matter per cone also that % dry matter indicates increased over the entire season, it suggests
=actual accumulation of tissue rather than a simple drying out process.
varied
4. While dry matter content/from approximately 15% to 30% over the season, expressable moiaturs ranged from
65%
357
.
Teets on physical and shemical propert:
=option of reducing sugars', indicated nature.
tion trends i The and et.
f refraction, us .associated with soluble' rose measureibly for all varieties.
2.
Sol solids followed almost identical curves as the index of ref ty: a property often associated with collodial protein tractile increased over the season steadily with Late Clusters and Fuggles, but rose sharply about a week before normal harvest in the cease of Bullions
6 For the three varieties tested for reducing sugar content of the juice from cones, three patterns were observed.
Only Late Clusters exhibited the expected drop with leveling off around harvest time.
3"7"-orws
"TM
9t r
reT
et
+d
*
*mg
a,95
WO Q9
6tt 0*
9't9
;a
C91
L91
(64 r9a eoa
Es
9'91
015
9'C9
Van
L*
95
T9C
TIC
5'61
) owni
TM
6C
6TE
9'66
Z9T
Cat t3Z t
Le
9ot
SLT got
Cu
OTZ
°St nal
9Z3
151
Ore* atro640tideellOWIS
Cla3
C*9
911
Z'9
4Z
LS eOT
5E
(Poll
55
90T
"(
L
6.6
OMNI
9aa
ES6 au eas9 a9
6t St* LCz gist
90'9
6'gt
84°C
'19 az az
EL
5 az
Cr il*
L
Olt
Loa
4'5
L9
93z
L5
0L
T'S
£'9
L
9/. 9
6g ST
Cr 19ST TT 916
OMNI
TTIta
0.7 owlatanotgioxpecis (p0w4aut
II taw. a r
6E
9*
5
16°C
Ta
213ni
*TT% on
90°T
65 u4"049 TTO 004n00
69°a
L trt
StiE tg*
$o*
4 nig)
/ ooT wina ure
0.7
96.0
Cco atm
2./.0
9L'z
6 Tr. art? a ti6°
9T 6 6E
To°9 raP
60a
(=n4
WT oCC
5ro
°a at
L
99°
E61
60E tyro
'E
SS
Lo°9
CrT
La°6 '6
C6°T oeo
L
9L'9
0.0
Toot
Study
a.
Fogg. 1.3430 1
Doll. 1.3414 2.3407
L.C.
1.3439
1.3439
1.3450
1.3458
14421 1.3409 1.3424 1.3426
1.3397 1.3403
1.3405 1.3412
1.3459
sp.
1.)456 1
1.3421
1.3418
1.3425
1
NNW
Terbitty (Juice) Klatt wilts filter 54
294 350 1068
Bell.
381 650 2900
887
968
3100
732
1412 1212
9000 7650
936 1597
2070 1870 2550
1750 2150 2050 2650
Solids (ass) % Solub
Pun* 7.4
Bull
L.C.
5.5
7.2
5.7
7.5
6.3
5.6
3.8
3.0
2.7
8.2
7.1
5.6
8.8
7.3
5.9
Reducing Sugars (Jell's) ag
.glucose / ml juicae
Pegg.
22.8
Bull.
23.9
L.C.
8.4
6.1
8.1
6.2
9.0
6.7
40111.11.
6.5
24.6
23.5
20.6
21.8
21.6
13.5
10.1
943 10.4
9.18
20.6
23.4
15.2
13.9
20.9
17.5
18.6
--
11.7
8.1
9.3
8.89
40.8/1, SAP
8/20
Expressable moisturs dr
%Mem rulteas
144
,V0,410,10%
8/10 9/9
/29 el2o
9
-
Pukes'
PA9 9
a
8/20 8/3©
So lublo Aids
- Bullion
Tug los
A -Lie.
Salim
0* ragtles
L.C.
8/30 9/9 fr19
shomical pr000dures.
relative Imo hops.
1.
of f GC.. and mhep acids.
Several reps and/s
Bach male flower ran from a sample of pure 47( accompanied by a covery de covering of 4 mg of P( ...avid ranged from
90%.
These were used as correction values for the associated determine..
or and aids in melts flowers.
A single recovery of pure gave only recovery.
As * consequence vomiter current distribution of male flower extracts was abandoned for the determination of theire-?( and ooutenta.
2.
Ion. exchange resins as a method of isolating and determining the ard,,
-.acid contents of male flowers.
Preliminary experiments indicated that Dowex40.formate vouli hold
-acids selectively from methanol solutions.
An early column of
200 mesh Dow x -1- formats 1.2 cm x 50 em was prepared and 1 ml of a methanol extract of Bullion hops (equivalent to about 5 mg. of o(sacid)eas added.
Thit was eluted with 0.2 N formic acid in methanol at 0.5 mis per minute and 5 ml fractions were collected.
These were made basic with NaOH and diluted to
10 mle with methanol.
Absorbency readings were made at 332 mu on a model
B spectrophotometer. Three fractions were isolated in this :tanner and their absorption spectra indicated that 2 were
-hop acids.
See figs. 1 ec
lute
P'Separationor <X7 -acids from methanol at of hops on ion exchange column of Dowexlwformete on solution; 0.2 N formic said in methanol.
Subsequent trials indicated that much better separation could be effected d by employing a stepwise elution technique.
Also it was found that the elution rate could be controlled much easier by using a coarse mesh resin.
A solution of pure ...acid (from the ortho.pbenylene-diamine salt) and pure,
(crystalline
9243°C) was prepared and separated as follows;
1 ml sample containing 2.7 and 2.5 mg of(i-.
...acids was added to a
1 am x 25 cm oolumn of Dowsx.104Ormola (30 neutral methanol and shielded from light with black paper.
50 ale of freshly prepared 0.1 N formic acid added and 5 ml fractions collected at about 8 minute intervals
(5/8 mls / min) Tintse were made basic and diluted to 10 mls with methanol.
Absorbancy readings were taken at 332 mu. Absorbanay at 332 mu was also measured for the original solution and recovery calculated
Semple
A3321 ml g08
Original solution
Fractions collected; y-aad al 87,6
.aoi4A17.7
extraneous 0
205,3
102 a QC.
a
(3 ..aoido
w000lution of the
2) with a dissolving in methanol.
Seigle
Direct N OR extract our.
Mb OR extract partitioned in pet. other
Thews results suggested that better absorption using the petroleum ether partitioning of a methanol that the amount of extraneous light absorbing material could be reduced.
In conjunction with the previous recovery of oC ...acid and (-.3 -acid absorbency, it was concluded that the loss of absorbency from the methanol extracts was not because of a loss of oC - or (-3 -.acids.
Treatment of resins from columns showing a low A 332 recovery with strong H Cl failed to remove any A 332 absorbing material indicating that this residue is extremely tightly bound to the resin or trapped physically within its molecular structure.
Prom these experiments it was decided to make ion - exchange separations of OH extracts of male flowers directly, rather than to resort to the more complicated method of partitioning into petroleum ether.
In a later experiment pure
6'\.
- and 1::' -acids were added to a male flower extract which had shown only of the added (_,{
-
55%-59% recovery ©f A 332, 93%
-acid absorbance was recovered.
A using the Aldo
A for hop extracts.
The preliminary results lists l b regression
Ver3r oua aging.
Methods A large sample of male flowers is ha
?MOH to give from 100 mg to 200 mg male flowers per al of141004 One portion of this extract is analysed for.aeid by the ion exchange method.
A second portion, (equivalent to about 2 grim of male flowers) is partitioned into petroleum other.
An aliquot. of this is diluted with all Me0H and read at 275 alp, 325 sup. and 355 mi on a
Beckman D
U.
Table /-f Analysis of Male Hop Flowers by Direct Spectre photometric method vs. the Ion Bxchangs Method
Direct Sport,.
Ian
Kgacict
1957 M
1958 M coOposite
5214 (Rep.')
1058 Ma 3171,2(Rapa)
1958 M,F, 320.1,2(1414.1)
0.36
0.16
1.31
1,32
0.61
0,40
2.
1.22
0.42
0.22
144
1.40
0.67
0.47
3.08
1.09
The fractional distillation of acide.
Bates (Chem. and Ind., No. 41, p. 1319, Oct. 11, 1958) has described a simple apparatus for the *fractional vacuum sublimation / distillation* of high boiling compounds'
Such an apparatus has been constructed and pre liminary results have been obtained for the separation ofe>,(-- andp. hop acids.
Briefly, the apparatus consists of an "oven" constructed from a plea'
50 416 ling end 2 es
22 niebroms eft e so that at o end t
0 to 0.5 tureefea. This was 'rapped with fiberglass taps re of asbestos paper then red with a larger piece of glass heating element was then connected to a variable transfomrr,
At 15 volts the temperature gradient over the length of the tube was free
150PC to room temperature.
The sample
045 mg 1140C-war/Si...acid) is placard in a tiny put into the sealed end of a piece of glass tubing $0, am long and
This is evacuated to the lowest possible pressure, sealed and the furnace.
Separation requires 1..3 hours.
The tubing is then cut into section', the sample eluted and measured photometrically.
At 1544) and 350p, presaure//,-;.acid was distilled from 0.3 al of a male flower extract containing the equivalent of 100 mg M.F. /m/ or about 0.3 mg ...acid.
The K- ...acid, however, remained with the residue.
At 120PG and 101.4 preswore,<er'acid was distilled from a similar sample but still remained with the residue It is anticim pated the or .acid will be,distilled when pressures less than one micron are used
It is also anticipa
CI lie collecting the essential oils from extremely small samples.
Collaborative rk with the U.S.B.F. on analytical methods.
Spectra hotometric analyses and iou- exchange analyses were done on duplicate samples of three varieties.
Results of the collaborative efforts are customarily reported elsewhere.
mat able moistere and stage of close relationships mere found, that each measurements could be used' such in the manner of soil moisture tests by resistance methods.
To this end tines preliminary experiments have been run to establish methods and apparatus.
They are presented below in their on form4
Electrisal Resistance Weasurements in Hop Tissue evaluation of K 01 electrodes.
Nei* s
To determine if saturated I Cl electrodes with a strinewiek contact mould make satisfactory-contact with plant tissue so that resistances measurements could be made reliably.
Apparatus nu glass tubing was drawn to about a 1 mm bore and out to about 6 can length.
A 1 cm string was inserted in the drawn end and the tube filled with saturated I Cl.
A #00 stopper was cut about 0.5 cm deep with an 8 mm cork borer and cut off at about 1 cm. A small hole was drilled through the center and a 5 cm length of fine Pt wire was inserted through the hole.
The stopper was than pushed down over the tube so that the tube fit into the slice made by the cork borer.
A standard volt-ohmpappere test meter with a 120, used to make readings. The probes from the instrument were inserted into the stoppers of the I Cl electrodes.
e1 resistance values, p=
A ) indica
Calculation of resistivity that the cross sectional area of the different stems accounted for diffe rent resistances encountered,
ObJeets Thin me probes which could be inserted into the stems of hop sheets would make it:easier for readings to be made.
These might further make more reproduceable contact than K Cl electrodes.
Apparatus, He 25 hypodermic needles were silver soldered to the leadends and insulated with tygon tubing.
silver solder
Hypodermic needle gon tubing
Latex tubing
Lead
Me de A piece of iksulating wallboard was ruled from 0.25 cm.
Hop shoots were laid on this board and one electrode inserted through the stem into the board at 0 cm.
The other electrode then inserted at intervals to 25 cm.
Resets:
Resistances were just about double those from the K 01 electrode.
Resistance was not linear with distance, curving upward.
The order of resistances between shoots remained the sane at all distances.
If these electrodes were used in subsequent studies a standard distance would be required.
to K Cl.
From the standpoint of manipulation these electrodes are far superior
1
3.
2
1
2 t NO.
1
2
1
2
1
2 tl
137
1614
110
125
140
125
115
132
100
174
200
180
165
210
153
189
290
310
215
230
270
240
220
260
220
310
290
320
290
470
370
1420
1400
370
430
370
333
163
160
160
160
134
135
125
155
160 tas of variation in resistance men ute between nodes, plants and s
In order to determine changes in the realm of ha maturity progresses it will be necessary to select a site of ve the most uniform results.
This expo what section of the main stem measurements can be made variation leas than exists between shoots or between plan
8 to 10 nodes have developed.
over
To Cate about
Apparatus: Stainless steel described in experiment u, were taped to a piece of wood in such a fashion that their tips were exactly 3 cm apart
Readings were recorded for the first 3 cm of each interned* beginning between nodes 1 and 2, about 1 1/2 inches out of the ground.
These were repeated on three shoots from each of three plants.
The statistical treatment is them nodes are the main effects, plants are within nodes and shoots are
among the o the various but that a* much plants.
00131IT'S plants as of the m ltiple range test suggests that imasurements made at any of the first Live nodes and still obtain less variation than would be encountered among shoots or plants.
Nodes (am iwats (within)
(withi)
$ 033)623
2 91/1
11444 IWO,
$90,t40,10
$92,624.1
89143244
.8
I
42
.4
62 ignifiosni ate 5% leval
7
K
996
1001
10ki
1073
1101
11Ik
1158
Means
110.7
111.2
116.0
119.2
122.3
223.7
128.7
Mg
P
121.39 / 9
(1)
2 3 5 6 7
.03
.9
348 3.27
3.33
3437 3.39
114
11.7
11.9
12.1
12.1
(1) Table 81, wintrad. to Stat. hotbeds"
Li,
529 (1957)
1
0
0
0
T
Cross
1.1
1.2
1:4
2.1
2.2
2.
243
4
3-3.
51
34
7)4
3344
13
4.1
89
44
49
4.3
26
444 27
5.1
54
53
39
5-4
9
94
8
6-1
6-2
6-3
6.4
81
2
30
1
3.54
85
65
15.3
22
1544 15
32
52
77
23
19
29
87
86 hit
20
16
12
97
63 at
59
91
506) 019032
1094 (209 4) 6190367
25$
119120
505 05) 019063
019076
03,9028
1 0190492
5-1) 051101m
01901401
01901011
121.2
25 01906211
Parontago
A x Z
WOO 2060
1063 2036
1082 2049
3059
20114 ion
2028
1032
20141
1052 2042
1077 2002
1023 2039
3016
3009
3038
3064
3057
1043 2035
1044 2078
1020 2059
101,6 2068
1012
1097
2046
2006
303 ma
3039
3093
3069
3042.
aut)
3036
3002
3071.
3063
A x S
A x T
1019 2083
1029 2089
1087
1086
2009
2064
30114
30014
3035
30143
1051 2023
1074 2071
1011 2026
1013 2032
3024
3001
3073
3059
A x R 1089 2090
10149
2088
1026 2062
1027 20014
3061
3097
3086
3098
1008 2013
1009 2087
1039 2086
1094 2076
3045
3032
3029
3096
A x y
0 x Z
1081 2024
1002
201414
1030 2051
1001 2027
3017
3053
30148
3070
1085
1015
2091
1065 2098
1022 20145
2099
3005
30314
3075
3090
4056 500a, wok
4022 5017 6015
4048 SaIii 6051
1 ol2 5019 6034
4035
14033
14051
5009 6037
5025 6009
5031 6018
4052 5059 6040
4003 5003 6032
4014 5028 6013
4042 5055 603.1
4041 5035 6002
14029
5007 6023
4060 5037 6052
4018 5051 6050
4021 5020 6005
4037 5047 6019
14030 5008 6020
4011 5050 6016
14046 5014 6010
4058 5022 6038
4057 5023 6026
4049 5027 6027
401.6
5033 6006
40314 50314 60414
4039 5024 6041
140145
5011 6039
11005 5013
60145
4059 5038 6035
-et
61
1-5a
£z tr-5z tffla
3-93
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93
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11
91
61
96
96
1
91
66
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8601
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L90z oLoZ otioE
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9505
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C1109
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5509
9509
14101
0501 goon
Trion ot(05 et05
1005
905
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11509 t0$(
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01iott
55 asos
1405
1E09
9C09
9209
119
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73
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37-1
374
37-3
37-4
69
90
404
64
404
140.3
la
404 62
D T
10614 20714 30149
1034 2080 3067
10142 20148 30146
1062 2050 3026
D
E
1
G
.1
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101
102
.6
-7
107 notes on parents:
Gode
Li
N
Late Cluster
It
N
4019
14006
4031
4023
4055
40143
4038
5016
5010
5057
5029
50014
5015
5005
6014
60149
6059
6025
6022
6033
A
Pedigree
106 019032 RV x
1409-2 019067 Bu x (EEO x
25.5
119120 Sun-S x K(iS)
505
3114
019063
019076
EKG x (SG x K0.5)
Fu.8 x (CC x rn.3)
212 C19028 EG EC.5
DM
Rea ass.
termed.
V. suso.
V.
!MSC 4,
SUSO ocCaeid
>6
<
<it
4
) 5
R
S
T
321.1 0190149M EKG x Bay.S
221.2 05110IN (rus x (Land -.3
x (ac x Fu-S)
(Land.S x (GC x Pitt.$)
317 -1,2 0190141M EG z X-S
Z
421.1,2 G190140 4 Fu z 1*um3 I
521.14,5 C190104 RV x Fu-S
1214 019060i EIG x Bay-.5
Suso.
311s¢.
V.
101130
Ras.
Res.
Res.
Maturity
Early
Early
V. late
Late
5 15
5
9
7 8 30
10
5
5
26 17
3
16
15 15
13 18 2
11
16
5 32 23 29
9 15 N 1.9
25
5 10 19
32
1.5
37
27
2
19
29 24 23
14
25 14
33
6 15 14 23 15
1.6
11
9 11
9 32
31 37 21 23 33
4
113
15
4 15 21
4 8 13
1 8 6
8 14 3
5 17
14
8
4 20
36 8 20
8
9
17 21
24 23
121
131 d
41 34 19
29 23 32
36 45
29 20
28 46
39 21
49
20 25
27
17
XI
26 34 33 31 25 24 21
25 14 11 17
9 19
9 20 14
32
3 10 29 16 18 25 28
29 IQ 47 19
11 11 10 29 33 27 23 34 55 65 17
9 10
8 13 17 34
30 22
37
41 14
28
33
29 42 al 38
16 33
42 31
45 23
25 35
34 24 27
30 23 25
22 13 23 26 22 30
24 33 27 40 26 33
31 33
49 30 34
9 29
9 42
28 32 8
45
26
37
31 7
25
36 45
21
44 15 46
19 24 24
35
20 35 33 16
141
30 47
34 28 21
9 11
18
10 28 22
21 40
1414
21 35
10 15 11
18 22 25 25
18 44 33 19 52
4 11 17
18
29 28
28 23
45 23
34 29
24 15 9
19
27
26 17
32
23 80
1154
li
26
11 24 15
20
214
18
28
2127
21 29
9 17 op
17 27 11
114 140
18
11 18 31
16 10 18
19 12
141
16 33 17
980 61.1000
432 466 489
51 220 272 299 558 523 582 738 702 860
312 31410
26140 14989
12195 21232 17962 10116
34250 114628
22682 31008 41320
1429114
8610 11023
120
33
29
36
26
42
29
37
314
28
35
2255
38
30
30
23
7
5
17
U
11 n36
17 12
23 14
25 13
15 21
37 8
24 33
28 27
27 8
23 2$
12 39 23
28 4
24 16 25
37 19
22 19 13 30 7
28 34 18 21 8
21 47 9
17 17 16 26 13
16 31 20
214
10
3 .3
18 13
19 11
23 11 37 16 23
38 23 21 38 14
21 41 24 16 16
39 1.9
18 22 9
19 11 31 27 16
10
214
21 15 12
218
22
23
21
U.
24
5
23
13
9
10
10
15
22
9
11
11
27
17
22
534 576 500 673 356 413
34
63 47
63
65
60
46
31
60
71.
76
40
41 26
83 67 49
71 57 143
68 91 41
58 61 27
54 74 68
62 63 47
50 65 60
61 95
48
50.58
47
7166
58
66 64 43
63 50 51
141
72
141
63 56 30
45 65 30
66 70 41
67
55
70 47
53
141
1148 1.6©3 1122 385 1405
45
15
11 35
11 9
27 23
17 13
14 7
18
114
25 27
19
12
13
21
114
22
9
18
13
12
19 15
12 10
114
22
1,5
13
15
314
114 214.
24 23
114
15
2
91/4 400 716
38578 21596
7168
130814
3.508
10896 19475
6382
7929 87960 532314 65113
106047 7653
7397
26 23 40 64 31 48
28 27 26
142
24 38
146
37
149
28 47
314
23 3.6
23
37
32
37
23 28
26 yr
44-4
61
39 42
142
34 32 59
24 16 29
314
28 66
21 4 6 30
25 26
214
24 15 50
34 19 56
43
144
35 38 29
29 19 29 60 140 50
34 33 33 46 39 32
45
45 32
25 34
1114
39 22 58
33 9 36
13 39 33
21 22 36
25 28 24
3o 25
143
38 38
35
34
33 22 37
35 40
68 36 48
69 33 38
22 2 5 32
20 28 42 62 32 59
19 2 3 49
36 23.
32
714
10
31 39
29 33
45 10 51 44 30 44
53 25 34 67 35 30
1170 852 1089 a 717 615 877
993 1164 eve
Se
22361 32749 59228 50265 44263 27398
17283 29986 58376
28
143
37
69 38
63
214
36 74 36
34 56 37
35 2 41
148
2
7 3 5,7
39 56
2 9
146
30
27
39 34
30 57
22
29
39 29 21
59 56
314
18
43 22
36 33 50
33 44 27
146
37
47
45
38
29
55
62
61 31.
59 34
7
40
35
32 17
23
29
19
27
2 5
3.6
28
2
34 34
24 17
4 21
15 25
25 35
34 24
28 28
27 24
26 17
24
214
24 27
973. 625 611
39871 15615
16767
47
28
36
43
141
35 37
43 42
38 43
39 47
29 36
37
142
39 2
36 29
35 35
28 28
33
34
2
51
32
32
3145
31
27
40
51
30
40
35
32
26
43
141
140
41
31
931 909 927
35769 355145
314631
32
21
17
13 32
18 9
24 19 27
29 1 3
3 2
17
28
23 22
16 16
24 26 17
21 20 24
20
19 24 25
19 33
22
16 11 14
34
214
20 21
29 23 28
22
18
15 26
21 18
15
11 21
26 25
26
19 20
214
37 52
48 26
1s9
54 63 41 26 23
3 25
3 26 29 32 48
35 45 53
14 14
59
6
22 24 38
26 27 39 a
42 5 57 95 26 35 29
59 36 47
25 39 52
50 27 72
83 50 64
85 46 70
59 50 56
35 22 28
28 16 21
49 18 32
35
144
39
79 51 62
32 58 30
37 38 63
68 49 65
78 65
146
30 30 27
27 18 41
15
314
12
37
148
63 28 32 17 35 39 62
58 41 90
48 43 52
55
146
37
60 65 51
31 40 75
141 73 59
57 43 67
64 62 52
72 63 51
54 72 71
35 32 15
37 33 28
33 23 33
36 24 27
32 23 44
1058 1107 1340
15148 1367 1597 810 668 697
47
43 39 s1 541 00 537
1114 945 20145
512
12739 12513
9594
149192
0453
761428 1008142 1014861
77463
18732
2 130 21589
551420 146237
37393
47 32
27 5 ils
57
53
57
38 20
4 1 4 8 5 0
72 51 31
32 53 41
36 30 54
35
35 48
40 33 53
49 36 51
52
28
144
148
414
44 3
35 46
32 20
33 59
51 37
27
0 the at Iamb Peel hilikalines, 1958.
(14)424
2 3
669
92
462
462
428
425
427
76
24
2433
652
7 1A8
95 61 275
79
86
71 294
64 290
8 89 318
7 46 264
287 3147 314
37
122 108 104
106 93 120
84 118 217
118
144
1:
128
319
3 3,
392
2965
39.5
611 207
29.4
931 909 ,27 2767
36.9
itl
© 116
I 26
1 109
2 129
155
134
81 810
X44)
0 124
(16)
4
92
1 28 21
1 66 63
2 77 65
3 67 72
4 87
5 92
66
91
Si 514 470
(19}417
2
3
79
28
97
20
80 123
110 113
118 97
13.8
115
135
332
3
102 318
28
82
77
211
70 212
70 209
90 243
95 278
Total
1 2
(18)523
3
Total
3.
(4) 3
525 491 556 1572
71 50 88 209
108 112 139 359
96 109 141 346
78 126 149 353
70 210 109 289
110 109 158 377
412 271 367
77 52 83
157
200 182
207 205 232
223 207 204
220 202 235
252 230 294
537 1548
20.6
1058 1107
13140 3505
146.7
15148
1367 1597 14512
60.1
Total
1050
212
539
644
634
657
776
Total
292
74
312
352
370
467
3.
(20)524
2 4 Total
228 166 219 613
66 26 42 134
162 155 138 455
139 163 133 435
189 158 178 525
169 326 152
1149
191 249 183 523
Grant
3.t._1
11855
2521
6454
6519
6561
6414
7006
Avg.
7.9
1.7
4.3
14.3
4.4
4.3
4.7
668 697 2175
29.0
2144 945 1045 3134
41.8
47360
31.6
outer surface of sepals inner surface of sepals anthers
103.1
26.5
214
106.1
25.6
9
107.1
27.3
1064 22.1
10 109-I 25.1
11 1124 26.0
12 117 -I 23.8
13 118.1
27.2
14 121.1
26.0
15 122.1
24.2
16 123.1
22.9
17 124-1 24.0
18 125-1 29.5
19 126.1
27.5
20 127.1
19.9
21 1215.1
26.6
22 130.1
25.0
23.131`1
23.4
24 132.1
24.3
25 135 -I
26.1
26 138.1
26.5
27 139-I 27.9
28 141.1
24,8
29 144.1
23.9
0
23.0
21.3
24.6
20.t
24,3
27.4
26.3
24.6
24,1
23.6
24.0
23.5
27.5
26.7
24.0
26.0
24.7
23.4
24.4
24.0
26.3
26.7
23.6
23.2
22.6
27.0
26.0
23.5
21.6
20.2
23.7
24.9
24.1
23.6
24.4
24.7
27.2
27.8
22.3
26.6
26.0
24.2
26.7
25.6
25.7
26.9
23.7
26.6
741.4
722.4
739.3
2203.1
19137.84 18094.04 18957.77
165339.69
IS..
11.6
78.1,
90.5
78.9
66.6
76.9
77.9
66.4
7140
61.6
73.8
76.7
74,2
71.4
71.3
72.2
84.2
82.0
66.2
79.2
75.7
71.0
75.4
75.7
78.5
81.5
72.1
73.7
23.8
24.1
28.1
27.3
224
26,4
25.2
23,7
25.1
25.2
26,2
27.2
24.0
24.6
30,1
26.3
22.2
25.6
26.0
22.1
23.7
27.2
24.6
.6
2 .7
23.8
25.3
ill
oh
144 kV:60
324
42.0
101
62.6
63
OA 31,6
27.2
19.14
22.1
29.1
22.5
15 in I kW
414.6
16 323 1
17 12k I
18 325 1
19 126 1
20 127 1
21 128 1
22 130 /
23 131 1
214 132 I
25 135 I
26 138 I
27 139 I
28 1141 I
29 1114 1
614.8
I 454
110.0
36.7.
1.8.5
86.0
51.0
14644
36.5
32.8
21.5
4.6
314
540 21.0
56.0
148.2
64.9
1.8.4
314.7
60.5
39.0
59.7
21.7
142.2
23.0
54.7
114.14
20.6
68.5
124
0.6
13 .7
4
47.5
147.6
1491
0
314.5
26.2
214 224
18.6
16.1
111.4
334.
took
176.0
17973.8:16
226.3
2514
MA
116.8
1214
830
064
280.14
1,910
114.7
1000
140.6
282.5
020
43
.8
35.0
33.0
51.3
119.4
27 .5
36 .k
36.9
e il k
22.8
564
28.0
240.3
53.5
66.14
260.7
10.5
196.7
32 4
116.3
204.3
167
Wel
90.0
1630
1770
279.8
1900
1340
1390
140.0
115.0
173.9
1180
(66.3) 2314 1580
4602 1454 21414.8
1660
32.8
208.3
11420
43.9
(40.9) 219.1.
55.6
54.9
11490
1670
27.6
14.6
11.8.2
1010
5998
62.0
31.0
1106
56.0
288.5
56.5
58.2
58.9
272 .7
1960
1850
610
31 1281.4 1225.4 12263 11117.6
1190.6
606.3
11420
Ea
2
643112 .82
61175.36445
Harvest Weights (11:124/1210t) in the Experiverntil
TWA Trial, 19544956 inolusive
Entry
Hep.I
1
9511
1956
1--rigg1es 3%0 134g
2 L c 91.7
34.4
168
53.0
3 101.1
36.5
142.8
48.6
67.6
36
5
4 102 I 49.2
50.4
56.8
5
103 I 105.2
460
66,5
6 204 I 48.4
24.4
37.7
7 106 I 54.4
368 47.9
6346.1
58.7
66 .3
1414.3
88.1
8 107 I 95.2
34.3
63.7
9
108 1 10908 8.8
42.9
10 1091 91.4
16.0
38,8
11
12
112 I 116.6
37.2
74.6
117 I 46.2
31.2
45.1
13 128 I 99.0
72.0
48.7
69.3
63.5
88.4
50.8
81.9
14 121 I 78.4
36.0
63.4
15 122 I 88.4
43.2
56.8
16 123 I 83.5
29.2
80.5
62.7
66.14
57.0
55.9
143.6
17 124 I 76.2
51.6
71.5
18 125 I 74,0 141.6
58.0
19 126 I 83.2
32.0
6348
20 127 I 101.2
17.6
32.6
21 128 I 83.5
54.8
5141
22 130 I 89.2
34.4
62.9
23 131 I 46.7
26.8
43.1
24 132 I 81.3
24.0
40.9
25 135 I 73.6
36.8
53.3
54.9
86.8
64
4
147.1
53.2
534
26 138 I 75.0
12.4
43.6
27 139 I 89.4
50.8
44.8
28
1.141 I
29
1144
73.2
91.0
59.6
70,6
60 47.7
48.8
73.0
92.3
146.9
HI
512
1958 Total
33.8
177.3
49.8
276.9
24.5
186.9
35.3
2514.8
65.6
3400
554
2314.9
51.4
232.8
WO 325.7
224 252.9
37.0
2146.7
64.6
381814
31.1
2104.4
61448 366.14
45.9
47.9
286.14
3024
36.7
286.9
86.0
3141.2
46.4
263.6
32.8
2144.9
24.6
230.9
5409
56.0
306.6
64.9
228.6
3467
39.0
3314
234.1
1.7
201.7
142.2
3004
514.7
35044
20.6
210.2
lg
.
100.6
36.8
140.5
28.14
5
39435.2
514,3
91.8
56.4
748
67.9
33.6
62 38 ea
59.6
99.2
14.9
5944 1444
56.8
52,3 1,2,0
119.8 WA 55,3.
86.3
38.1
3143,
11592
129.1
104414
128.2
140.0
103.0
143.6
60,2
42.0
72.1
56.5
82.0
33.6
65.7
83.5
300
24.0
109.0
53.2
65.6
37.6
70,6
80.9
627
3%3
71.7
400
6144
81.2
60.7
63.7
53.6
92.8
4..0
34.6
37.8
86.2
56.14
3906 6262
73.6
143
674 28.0
68.3
61.9
69.0
25.6
16.14
Ma 6245
148 .9
7803
58.8
29.0
594 34.8
454
140.6
2
370
89
88.0
148.o
57.0
73.9
86.8
73.0
66.4
640 66.7
544
76.8
103.h
814.0
146.8
147.2
61.1
82
9.2
364
55.7
294
53.7
21.5
31.3
21..0
he.2
USA
6%5
59
68.5
WWI
124
2282.2
982.8 1543.5 1756.7 1288.11
7053.6 2427.3 1122.8 16674 00%6 1225ek 11$24
192887.94
86552.47
40566 56
64342.82
113620.43
218607.23
2213082.82
100708.
5052664
61175.36
2243606 th55517.0
Co'
vatitOPI cteei
Mit
AOAIRV1 lwAUSWIAMA samgenteaqictnilvvit nill olsttas egPae=akiiiAReM22APA
'11'2"IuetiTilm'-°°1-1:141111-41411 ma,h(smogm... iiii4igftaaAsovz innn4n0001
SAACA''a4in aPA"""1410,53gAPt2°'
A llavvvrinctn.d.AnlarinvponwT0111
.n
geM12gg436(22'32AR=7**Ae4A g v7In
114.1s1.,21;;IIgm111,dri .Amps
1W52Vont$1=Wilga34120AVAAW.2
cvin1150.tinninivinsiilinnnonno.
mmgig°:mcalAw4ggvt(gr-a142-4 vierv11:111 p-wp-.v3Au.
nwillvilcmlmcuctct
HNHHHHNHMHHHHHHHHHHWHHHHHHH
ROW4n5R4M°455
N
sic sx2
819.9 3,540.4 6,558.4 6,9944 4885.7 31.798.6
3,494933.63
1,521 340.52
490,00946
892,079.09
1083,624.30
35 870 051.28
130
28
2
56
86 Total
Harvest weights (
Lim
Rope.
Total (1as)
) 109
11
14
28
19584
202351036
369.655
15,148.505
,169.196
726.8227
ligfr
9244138
1141.7291s
Ramat weights (lba./Plot)
95144958
Linea 28 50,138,837 1,790.6728
Blocks (yrasuld reps.)
19 202,473.638 10,656.5073
Error
532 U2,493.978
2114548
Total
579 365406143
131
1 'L.C.
2 103.1
3
1044
4 107.1
5 108.1
6 109.1
7 122.1
8 223-1
9 124.1
10 127..I
11 132.1
12 135..1
13 138..I
14 139-1
15 144.1
16 B.G.
sx2
I II
24.1
24.0
26.2
224
25.4
27.4
22,5 21.9
21.9
26.1
25.5
254
28.8
23.2
27.3
24,1 23.6
22.7
22,7
260
25.9
23.9
25.0
24.9
23.6
22.9
24.1
22.7
25.8
29.7
26.1
28.4
27.5
25.6
24.1
25.6
25.8
25.4
26.3
28.4
29.8
26.9
24.9
29.7
25.3
31.2
25.3
30.3
408.6
409.1
4060 122567
70.2
79.0
66.3
76.7
76.5
68.6
79.2
73.8
70.6
80.8
79.4
75.3
77.5
85.1
75.5
91,2
1o506.44
10484.32
10563.37
9 4478.31
As
23.4
260
2261
25.6
25.5
22.9
26,4
24.6
21.5
26.9
26.5
25,1
25.8
28.4
25.2
30.4
Source of Variation
Lines
Reps.
Error
Total
2
30
47
194.01
.04
61.32
255.37
M
12.93
**
.02
2.04
ilorrest, OorililliaPriste
1
1$8.0
524
49.7
5 108 x 7144
6 lop I
45.4
7 112 X 149.8
8 1231
45.2
IP
124 I
10 127 I
24.5
39.9
11 132 X
12 135 I
52.9
314.4
13 138 I
14 139 I is 314 I
18.1
37.7
26.8
16 Bros Gold 1.9.6
SI
512
29.7
34.6
45.0
21.6
47.0
19.0
28.4
37.1
60.7
28.7
30,5
55.8
621.5
28392.03
590.2
214125.24
37.7
.6
32.0
36.2
40.2
30.2
27.4
58.8
18.6
53.14
663.4
30312.64
1883.1
2376714.25
75.8
141..8
1614
1,140.3
11450
104.3
170.8
109.4
87.0
105.8
127.7
109.6
67.1
1143.5
614.4
128.8
1830
1590
1640
1180
19140
12140
990
1200
1450
12140
760
1630
730
1460
1330
133
a in the Co
1956
Rep.I
1957 1958 Total 1956 1957 1958
T
Re III
1957 1958 Total
58.5 55.9
114.0
128.4
48.4 83.9
2144 156.14
29.0 55.8 37.?
122.5
1 L.C.
2 103-I 31.2 60.2
48.0
139.4
40.9
73.8
141.2
255.9
28.3
54.6 52.6
3
1&
38.0 44.5
52.1
1314,6
26.5
107 -I
334 53.5
39.9
554 56.2
151.2
49.1
74.7
19.7
173.5
39.1
74.8 33.3
1147,2
28.5
94.0 57.3
179.8
5 106.4
464 50.6
71.4
168.1
47.6
59.1
2E1.4
135,1 604 62.2
454
6 109-1
7 112 -I
43.3
86.7
45.4
175.4
21.6
60.14
37.1
119.1
31.5 38.5
21.8
91 8
37.7
95.9
49.8
183.4
30.9
82.14
60.7
MA
214.6
58.5 60.3
140.4
8 123-I
514.8
80.2
145.2
180.2
66.1
51.0
28.7
1145,8
78.9
69.0
35.5
183.1
9
10
11
12
19144 35.9
43.4
214.5
103.8
59.6
46.4 30.5
136.5
36.3
55.4 32.0
123.7
127.4
21.4 30.7
39.9
,2461,
46.2
25.8
29.7
91.7
29.9 13.7
36.2
1324 39.6
71.0
52.9
163.5
33.1 52.5 34.6
1204 264 4640 woe
404
112.3
135 -I 27.1
147.8
344 109.3
26.2
53.9 45.0
23.7
52.4
30.2
106.3
13
138-I
31444
27.2
14 139 -I 31.14
184
7967 48.9 43.0 21.6
113.5
35.3
434
27.11
106.4
604 37.7
129.2
28.6
624 47.0
137.7
25.2
524 58,8 136.2
15 1444 35.2
71,3 26.8
133.3
23.1 60.0
194 102.1
48.8
59.0 18.6
16 B.G.
29.0
51.14
19.6
1004 29.6 36.7 55.8
122.1
2841~
45.0
53.4
126.8
81 612.7
951.6 629.5
2193.8
606.4 898.9 590.2
2095.5
572,1 885,1 663.1$
=2
214973163
28392.03
62244.68
317999.50
25566.36
21425.214
54840.39
28151103
23938.39
30312.4
530249
291783.86 26360803
1
83.9 7
55.8
195.6
188.6
132.7
243.5
171.9
185.6
236,8
200.2
7 71.0 47.8 *7.2
pt!
53., 143.0.
7 46.0 52.4 43.7
2
169.5
154.1
113.9
71.3 51.4
60,0
59.0
36.7
1904
24.3. 41.2
374 52.6
524 49.7
7144 115.4
53.5
33.3 28.4 37.1
06.2
574 45.2 21.8
49.8 45.2
60.7 28.7
60.3 35.5
39.9 52.
30.5 29.7 346 1154
32.0 36.2 40.2 30.2
184 37.7
21.6 47.0
27.4 58.8
26.8 19.6
19.0 55.8
18.6 53.4
75.8
141
161.8
140,3
1454
104.3
170.8
109.14
87.0
105.8
127.7
109.6
67.1
343.5
64414
128.8
11/8)A
407.3
430.8
398.9
500.5
471.3
386.3
497.8
509.4
364.0
2$.5
340.7
299.6
403.1
361.8
348.9
62147301 54687.89
74413.97
93382.92
46023.28
547 5.95
31541,58 51831.86
65757.36
92595.23
54618.81
91968.1414
25889.93
41687.97
58266.65
14417)4,05
14237970.41
Method)
2.03-
107.1
100.1
109-1
9
10
11
7
8
5
6
1214I 3.2
127-1 13
132/
14
135.1
3$
138.1
16
139.1
17
144.1
18
1
2
3
5.84
5.78
5.02
3.27
1.53
4.65
3.20
3.140
3.76
8.1,
5.$2.
4.28
8.55
7.59
2.83
7.25
7.814
2.95
3,10
4.20
3.19
445
2.88
14.114
4.83
4.39
4.80
4.99
5.18
4.52
see 112.1, X.Y.T., below
7.98
3.46
3,08
4408
3.90
5.36
5.87
1.49
4.32
3.58
3.64
5.62
2.75
5.48
540 5.09
2.01
241
1.42
3.92
2.78
...
5.73
2.84
545
405
1.72
5.08
3.51
3.19
112 -I
128.1
* Fro* itepello
5.07
8.99
5.26
9.17
674
9.36
4.69
8.73
19.76
36.25
2, 3, 4 or Experimental Yield Trial
23.06
16.85
21.16
12.54
6.16
18.97
13.07
10.23
17.67
1,5
31.97
154.43
9.24
22.25
16.57
17.92
20.05
9,06
5.76
4.22
549
3.14
1.54
4.74
3.27
3.41
7.71
3.06
3.06
4.14
4.48
5.01
a Cis
15
16
17
5
6
3' a3.1ion
1044
7
8
9
107 -I
108-i
109-I
1124
1234.
12
13
12144
127.1
132 -I
114
135 -I
1384
1394
111144
1284
.93
2.94
2.3$
.73
1.11
.80
.85
.96
.96
.22
.75
.97
.52
.69
2.37
.67
2.49
2.61
.83
.65
1.06
1.03.
«65
1.28
.92
.73
.90
.22
.83
.65
142 835
47* 1.57
1.23
.88
.66
.86
.83
.65
.67
16
.30
1414
.33
514
2.03
a-
2.3.0
142
.64
.52
.59
2.15
WOW 0( than others Probably nixed either r sampling.
Not used in avg.
T.T.,, hills 420 & 4.
0,59
2.60
243.
0.78
1.17
0.83
0,49
1.30
0.60
0.75
045
0.914
0.21
0.85
0.64
0.10
0.62.
2.1,6
the Corvallis4Proosor Tiold Trial, 1958
138
12 I
Total 34
2 51
3.3
8#
3.2e
15
35
0
18
14
19
21
0 0
9 0
33 21 n 31.
32 21
22 14
14 17
3 51
8,
148
52 Total 63 54
11
U
0
90
15 25 35 39 121
Total
37
43114 73
14
17 0 7 0
8' 30 15 0 9
12# 36 32 39 22
Total 83
147 146
31
5 51
21 6 (6
29 31 (29 (31
12'
33
Total 83
78 83 78
6 51 0 o 5 0
7 3.2
32 13
12' 8 12 25
U
Total 15
214
62 24
7
5' 39 0 0 31
81 24 13 11 24
12' 24 19 10 23
Total 87 32 23.
78
8 51 0 0 15 26
81 21 20 0 36
32t 30 31 20 26
9
Total 53.
53.
35 88
5'
12'
1
0 0 0 19
11 6
10 5
Total 21 11 33 37
Rep.12
3 4
Grant
Total Total
8 0 0 6 8 r
6 0 12 23
21 18 10 50 26 104 17 37
66
0 7 63.
30 23 34 26 93 38 32 36 10 116
39
186
275
95
50
103
114
28 33 90 54 205
6 0 0 0 6
47 21 33 16 117
40 33 41 27 141
60 75 36 29 200
8 0 0 0
20 15 30 4
8
69
30 23 40 27 120
500
60 33 3 6 0
70 14 22 18 13
87 27 18 20 9
42
74
12
57 13
12 n
22 1.8
7
13
11
24
142
62
34 20 74
344
189
235
568
217 74 33
144
22 173 47 52 34 45 178
64
289
375
728
267 93 54 74
143
264 58 38 70 31 197
24 29 5 25
54
129
0
29 10 20 9
59
68
26 16 32 145
119
12
8
8
7
15 20
17 26
55
58
26 32 40 51
1149
138
180
397
715
207
54
120
148
814
31 77 54
2146
214
0
14
0
214
0 10
28 35 11 13
28
34
87
46
147
72 97 262
36 12 0 2/4
72
26 22
34
16 33 97
29 20 24 107
322
5
614
56
52 59
13.
27 149
0 0 o 36 36
4 15 30 25 74
21
214
17
141
103
96 63 36
83.
276
0 0 0 0
20 12 13 24
13 9 20
142
0
69
814
125 25 39
147
102 213
70
72
76
0 8 0 33
13 30 10 16
18 28 9 21
33 21 33 66 153
43.
26 38 0 34 98
69
3.2
76
20 16
3.9
19 25 17 38
67
99
218 31 66 19 70 186 57 83 33 91 264
Ia.
77
107 o 0 0 0
140
143
0
18
22 29
0 15 73
24 118
0 0 0 0
25 214
32 14
23 32 13 16
0
95
84
225
12
_.5
83
140
29 39
2 2
8 z 8
19 18
12
20
10
22
191 48 56
145
30 179
41
60
9
12
9
1k
13
.0
10
18 22 62
102 19 18 51 50 138 30 33 29
143
135
154
251
342
7147
41
207
243
1493.
209
208
251
668
41
245
309
595
167
375
loz
10 51 es
121
0 15
9 45
29 22
18
37 lb
69 75 255 71
10
18
11 51
12'
0 0 37
32 10 19
17 29 43
17 11.
6 47 42
6 95 33
8
0 0
5
26 15 17
Total
29.
39 79
225'
0
Total 18
0 0
8'
0
12'
8 14 20
12.
159 86
5
12
12
26 20 29
54
93 31
145
42
27 22 180 o 0 0
14 8 49
21 25.30
6 82
44
3.14
131
0
27
37
9
8
6
0 0
5 5
7 10
13
29
37
13 51 0
8' 6
12' 8
0
8
10
0 0
6 7
13.
8
Total lb
14 51 9
81 28
12' 19
18
9
18
38
17 15
22
36
0 27
33
18
Total 56 65 58 78
155'
0 0 8 0
8'
12'
10 10 10
9
12 12
Total 25 22 30 21
64 23
45
101
22
31
Ul
26
257
26 8
27 28
20 34
79 73
30
36
38
70 104
8
39
51
98
12 15 29
6 6 22
9
14 11
9
29 26 26 13
44 46 59 22
79
86
122
118
326
314
143
94
171.
23 30
30.101
146
94 271.
0 5
18 12
21 32
0 o 5
17
17
20
35
67
105
39 49 34
55
0 o 0 4
20 11 17
26 19 22 18
9
177
14
57
85
46 30 39 31 246
0 0 0 10 10
8
9
16 14 15
22
53
16 14 61
17 38
7 27
20 23.
15 26
42
0 8 12 4
13 7 9 7
24 5 5 8
37
30 39 124
26 10 70
36 24 101
39 20 100
74 103.
54 271
214
36
42
20 26 19 102
704
184
291.
516
9
140
221
370
23
109
135
267
201
324
329
854
66
118
187
371.
16 51
8 6
8'
33.
18 10 11
12'
8 6 4
214
45
70
42
24 17 10 22
29 26 23 19
19 29
73
97
94
10 13 16 0
27 26 33 13
29 39 33 18
39
99
119
157
266
255
Total 53
111
22 41 3.57
72 72 58 62 264 66 78 82 33.
257 678 sx 698 653. 759 753 2861 895 681 786 732
30914
752 858 746 836 3192 9147 sae
ST2
15644 19643
15359 18427
214949
20480
14839 18166
167914 19220
21662 22102
39880 147827
33339 146333
601467
60793 47250
34223 45208
652556
140362
42170
514432
2192805
53764
690040 5650499
L.C.
143.4
104-1
107 -I
108.1
109-1
112.1
123-1
124-1
121.1
132.1
135 -I
1384
139.1
144.1
sX sx2
156
3.324
1095
1323
2086
3.140
1533
1911
1156
1 060
749
3256
987
1876
1805
1
21159
1951
Data from the USDA
Hop varlets]. Trials in Cooperation with the Irrigation Experiment Station, Prosser Welk.
(tram C. Z. Nelsen)
Pounds pe
RsPa
!tail
.!!2111
2212
1953
720
1636
1820
778
1492
1305
1194
920
921
855
1350
1393
2530
89k
749
11156
1538
2120
1080
3315
1213
( 495)
736
350
894
2h34
1302
2009
1369
824
1417
2258
1012
1035
1.302
845
100.3
502
1124
1598
1327
1.558
1728
690
1305
1902
1449
1437
2614
2437
251
928
1069
941
2578 lobo
--EA
1211
2049
1454
814
1427
1921
1100
1315
1681
1169
495
869
620
960
1767
3 flowering Debi dab' ar"4444
18967 17066 17956 20027
157543 27537797 23224328 254308i2 34952723
6881 911,771
* Not harvested*
Fertilised Mar* 19-22 2 t. alfalfa/a plus
Banded 120 1b3.
immonlun nitrate*
1958 Data from the USDA Sep Varietal Trials in Ommummties with the Irrigation Experiment Station, Prosser Wash.
(from C E. Nelsen) acre
11.22.1.4
La.121
LI/
Flamering
Lira
L.C.
103-1
104.1
107 -I
108 -I
109-I
2432
1879
954
888
1659
880
112-1 870
123-1 1789
1244 691
127 -I 328
1324 783
135-1
138-I
685
868
137.1
1252
144-1 1006
3018
2388
865
842
1653
1484
1231
1699
1057
382
1.141
830
693
1382
1042
2872
2118
850
1042
2097
996
1099
1989
1045
461
1101
643
993
1177
1170
2741
1991
970
1137
1673
880
964
1623
1503
333
1203
824
1567
1042
1351
2593
1556
822
902
2370
1906
1075
1912
1049
389
1175
832
558
776
1592
19653 20,302 19727 SI 16964 197©7 a
2
23628370 32334575 31.766393 32311122 31857593
1081
763
936
31
1332
Fertiliser side dressed in
14 bands 70 deep April 10.
100# Eta as ammonium nitrate
101 ZN/a as situ) ammonium sulfite
671 P201.
as treble superphosphate
Irrigated every 2 weeks
1234
12144
127
132.I
1354
138.1
1394
114144
L.C.
1.1c7
3008
4.14
3.05
1.95
1.90
1 .87
2.75
2.145
1.73
4.78
1.77
78
2.27
3.214
5.22
3.00
2.18
1.82
2.11
2.68
2.22
1.83
3.06
2.68
2.91
3.146
628
680
1.57
1.61
1.55
2.87
2.49
2.81
14.811
0.66
9.78
13.914
10.85
5.70
5.33
5.53
7.30
7.16
6.37
12.68
2.89
3.26
14.65
3.62
1.90
1.78
1.84
2.43
2.39
2.12
14.23
107-1
1084
109.1
1124
1234
124-1
127-1
Bud
L.O.
.29
103-1
1044 .28
132-I
135-1
138-1
.91
ash
.30
.32
.15
.40
.19
.51
.52
139-1
11414-1
.35
AO
Oil Coated': OorvolUisowtrosser (Prosser), tAtisACO pest* bow tby bads )
.26
.Lit
.30
.147
.29
.32
.21
ftti
.29
AP 46
.25
.25
.12
.35
.15
.29
.63
.89
.21
.22
.39
la
.87
.37
AO
.20
.36
.52
.se
.33
.29
.15
.28
.51
.22
.1a3
31
.31
.18
.33.
.49
.29
.29
.145
.38
awes al Variatifa ate., Corvallis
15
Total
30
47
167.749
3,437.411
8,953.543
114.5)30
Linos
Reps.
Years
Limonx rears
Grand total
2
30
64
143
15
2
30
3
108.688
1082 961 1.36.099
11,299.259
5,649.630 *I*
10,731.771
357.726 **
6,746.423
105 413
40,550.525
Lines
Error
Total
15
2
958:
11,
02.0
301.1
558
575
70,025.6
32 028.7
780.13
41*
2.50.55
125.49
Yield (lbs./i), Prosser,
19572
11:
Reps.
Error
Total
56
74
280
864,991
5,243,857
2(4234128
1,008,734
41*
216,248
93,640
rita1 its4ressrp
Analysis ot
seri
1958$
Ilt
56
711 l ,278,325 4734166
461,945
114146
72,42
60,222
**
lei. all per
MR Ai 4.11,
2.$ «
*Pro bla0 Iielertaa
0.714 dry
0.94
%OS last
Goli sang* 51,
1t.37
128-I
135-4
MIL
4"44441
6.75
206
5.00
5.70
6
5.26
514
Backe
B 0.
L.G.
103-I
1074
108-I
112 -I
1351
139-I
114-1
(%a( said, .was, Colors metric 'frothed angle replication. 5 hill plots
5.63
8.014
8.03
2.20
4.214
7.09
6.24
2.63
14.27
3.52
Sacramento
County Hop Genetic Block, Bradley Ranch
Readings taken August 11, 1958 one week prior to harvest
(data from lift. 0. Golden, Jr.) h 8, 1958 - Flooded three times before emergenosi
Vines per Milt Number of vines trained on three strings. 0 means hilt miseing, replanting necessary
Average arm lengths Estimates in feet.
Relative puma yield: Readings based on Early estimated b plants in alterneating yield 7 -9 bales per acre.
Readings based on Early Cluster baby plants in alternating top-set and very adequate middle and bottom met.
NO positive mildew symptoms rated in genetic blook; field heavily infested during most of season.
within 1/14 mile wile
Genetic materiel to be hand harvested and replications bulked ecoording ter select/am'
Hops to be forwarded to Stan Brooks, Oregon State College.
Vines
1
2
3
It
5
6
4
6
0
0
3
3
1 -3 x Fa-5) a a
I
1
2
3
4
5
1
In
2
2
0
0
0
5
0
2
0
0
0
4
0
0
0
1
2
3
4
5
1
2
0
0
3
0
0
14
0
0
0
2
4
3
1/23
Relative gemsral zield
(Bel 31.3 x
2 -3
3-4 1-2
G x Bel 31)
P
SPind2.7 plant still SA %UV
Small cones, lets,
3.
vi numerous browsed airso nigh (spikes?) felimssiztlaigifgft.
Rep.
It
1,
2
5
1
2
3
14
3
2 p.
1
0
3
0
Aw r. a lent c
(13)
Relative general
noel to
.
mire
112 Sam x (Bel 314 x Bel 31) sent.
1-2 312-.1
P P P
411401OmmbleOwt set below 6 ft.
Spindly plant. Wm arm & vines
2.3
4
P
P
2 ft. below wire
1
2
3
5
1
2
3
0
0
5
1
4
0
1
I
0
0
0
1/2
3
2.3
1.2
3
3
P
0 a
X x U a
P1
P
Rep.
5
1
2
3
5
1
2
3
5
1
2
3
14
3
5
1
2
0
6
2
5
14
5
2
0
5
0
0
2
0
2
6-7
Avg.
1/2
6
Relative general.
J4
135 -I Fu x (R7 a
Unbmown 3)
1/2
3-4
2 -3
COPRIERCIAL INIERPIARTS (La
3-4
None
0
(7 139-ix Barr x (Bel 31.4 x Bel 31)
3
2-3
3
2-3
2
3
2
1/2.1
1/2-1
2.3
2 -3
1-3
1-3
1-2
1-2
1-3
0
P
K
P
N
K
P
K
K
P
K
PI
K
ANIONOWNIONIVINOW.
X
0
P
Vigorous plant
A tow lemma blare
1 vino not to wire, e tbors just, to Ids*
Some burrs
la
5
1
2
3
1
2
3
4
5
1
2
3
4
Hill Vines
No.
per $1/1 arm len ground to
6 ft.
(8)
6
0
2
4
4
1-14
4
14
1/2..1
3
0
3
2
1-3
1-4 1/2.1
0
2
5
3-4
2.3
2 -3 1
Relative general
Yiald
Cone distributicn ground to 6
6 ft.
mire io
P
P
P
P
P
P
P
N
N
P x adjoimmg) adjoining)
1
Early Clusters adjoining)
155
STANDARD FORM
MEMORANDUM OF UNDERSTANDING between
Name of Grower
Post Office Address and
The Crops Research Division,
Agricultural Research Service, United States Department of Agriculture and
The Agricultural Experiment Station
The purpose of this informal cooperation is to test promising selections of hops.
The plant material noted below is furnished the Grower and accepted by him under the conditions cited in this memorandum.
le The above cooperating agencies agree to furnish to the Grower the following plant material:
2.
The Grower agrees to grow the above plant material in the following location(s):
3.
The Grower agrees to furnish the land and labor and meet all expenses involved in caring for the plant material and the harvesting and disposal of the crop.
It is agreed that the Grower may dispose of the crops as he sees fit, except for propagating purposes.
Representatives of the above cooperating agencies are to be permitted to make observations in the field from time to time and to obtain samples and propagating material from the plants when desired.
4.
The Grower further agrees not to propagate additional plants for his own use, nor to sell, give away, or otherwise distribute any of these selections until authorized to do so by the above cooperating agencies.
156
5.
The Grower agrees not to dispose of the land where such plants and/or propagating material are growing without first destroying such plants
2 unless the purchaser becomes a party to this agreement.
6.
The Grower agrees to permit the representative of the cooperating agencies to destroy this material if it proves diseased or off-type2 or if it is discarded.
7.
The cooperating agencies will furnish plant material believed to be free from serious diseases or insect pests.
The Grower further agrees that he will not hold the above cooperating agencies responsible should insect pests and diseases appear on the plants.
8.
In the event that the cooperating agencies decide to release any of the selections 2 the Grower agrees to distribute at least 75 percent of the cuttings for increase purposes to individuals designated by the cooperating agencies for a period of two years following the decision.
It is further agreed that the
Grower will sell such cuttings at a price determined at the time of release by an allocation committee of the cooperating agencies.
9.
No memb er of cr delegate to Congress or Resident Commissioner shall, be admitted to any share or part of this agreement or to any benefit that arise xherafrom, but this provision shall not be construed to extend to may this agreement if made with a corporation for its general benefit.
10. The coope-:°ating agencies designate the following representative as coordinator between the zooperating agencies and the Grower.
He will be responsible for the distribution of plants and for directing the evaluation of the material
Name
Address
Date Grower
Date
Directory Crops Research Division
Date
Directory
Experiment Station
Agricultural
desired plans testing of enpsasinoiatal help varieties in Orogen.
San may be necessary duo to liaitstions in trellis sise or shape
Any sodifiaatifens that do appear to be nee*
*red sines they may reduce the precision of the trial. Otherwise of fertilisers tivation, spraying She use will be consistent with the pCraetWoes in dement use by the grower-coo
1. Varieties to be tested*
112 I (0 19215) Sam z (Del x Bel 31)
128 I (C 19113) Du z (Sioux Unknown-5)
135 I (C 19151) Fn x (RV x Unknown-0
114& I (C 19077) iF z (1,0,xyg.$)
Tuggle'
19209)
2.
Planting stocks
A minimum of lop hills of each riety will be rem red in o sufficient hops will be available for large-scale brewing trials.
Planting stock of these varieties will be provided by the Collage under a mismoraNhalof understanding between the grower and the college.
3.
Fieldiplot diagrams
The locations of the varieties in a trial will be deterrined at random.
The plets will consist of single roue grown side by side with each series of the fire varieties being located adjacent to another series or replication.
The planting of border or guard rows will be permitted around the entire experimental area.
Guard rows may also be grown between the replications, but not within replications.
I 48 should lie I,aisi oat sash saw that theiir disessiisli a of slops Sr other Nil gisAients. Stringing and training should be dens in a manner whit% feeilitates harvesting of single plots ens of the following plestisg plans may be used
!bra particular trial (plots namborwitrem the leftleben teeing the trial from the Meese roadway), When three replications are used the plots 'mill be 35 bills lion0
When twee replications are used the plots will be 50 bills long.
!mg
Plan I asp= RepIA
103
0
I
102
201
205
202
Plan la
Repa
Variety
H22,14.
112 1
128 1
135 1
144 1
Tuggle*
102
101
105
103
104
204
205
201
202
203
305
301
304
303
302
Plan III
&spin 11.14'4
Variety
1121
328 1
1.35 1
144 I
Tuggle*
103
102
101
105
3.014
205
203
20k
201
202
305
301 ing and dryings is
Harvesting of the experimental plots is to be ac equipment of the grower-cooperator.
A college representative will be present at the time of harvesting and drying to assist in beeping the yields from the individual plots separate until a measure of the yield has been made.
He will
The also obtain samples at the time of harvest on an individual plot basis,
the drying vaxi
balm simples are is
s ism order that they can be
Data to be obtained,
The min *bleu, ram y, disease mistiest and picking ability. A minima et tefOre these cheviots stics of data (ii years actual) is ooneidered yearscan be determined with any degree of ee ye
Yield will be determined on an individual plot basis by co ting.or
'sighing the sacks of hops ebtained from thin. Samples will provide information og dry -down for adjusting yields to a demon moisture content.
b.
Quality will be determined by laboratory analysis of the samples provided on an individual plot basis and by brewing trials.
Baled lots of each variety will be provided fray one, too, or three trials each year Shich will be submitted for brewing trial analysis.
c.
Disease reaction will be determined by observation throughout each season in conjunction with more detailed studies at the college do Picking ability be determined by observations on shattering, leaf and stem content, and ease of picking at the time the plots are picked by oommercial equipment.
The release of any of these experimental, varieties to the hop growing industry will be determined on the basis of their performance in off station trials and their acceptance by growers and brewers.
6
SI
2
Treatments
Replications
Error
Total
30100
70
II
.4
17.3
24.9
23.0
28.9
32.2
342 33.4
244 20.9
27.3
16.2
21.1
24.2
"25.6
22.3
21.7
15.6
24.9
20.0
.1
109.6
131.7
11403 11769
2892.95 2576.34 3523.91 2777.29 46346.67
720
1070
750
760
730
4
3
19
MS
77.542
53.546
17.249
130.876
10.906
494.590
SI 20090 3084 362.8
328.7
311.5
354.9
#95.7
liar
8554.54 19969.47
27U0.50
22299,37 2062941 256117.39
3.8605.63
2162.5
96809
Irrigation levels
Replleations
Subtotal a
Fertiliser levels
I x
Error b
Subtotal b
6
24
90
0
4
3
12
19
1,1,6 292.3975 *
273.467
91.1557
690.625
57.5521
2,133.682
872.672
269.738
11.2391
4033.896
11.4877
4176.306
4.309 988
Note: The harvest wts
on the basis of 25% dry-doun before moisture data were a dry-down was later determined to be 28.6%.
table were computed b The average
The yields in the to ch, in pounds
t96,1 aCC
6St
60*6E rtr /C
ErE
Won
5
92411
Cr at
CrE
WC z
Lo£ 69a
aim' lir a
ic
LosE 5£C trier
C
IC 91 tirCI
8L £t
VITT
05C 99C WC tiLC
Iraq IrrE
99TE
59°C
90°C
99°C
E
ITC
/47* a
95C't
CL trc
arr gEC
sec or £
9PC
Irv(
oc cri scc
II
C t a
ETT
tat
Plrivintott irialP6)00 a col/41-Pi so asTIPAA t
9e6 I a
C
0a.6 lrot
9o01 oot aPot
56'6
9
9r6 a
In6
09.6
Te6
Trot
Ce9C nC.6C
8911
/*ol C9651 evaltmay
Jo eaurralm v$TaaT uo ILI:rm.zos °P1-0,)0
21T.2.11 de% u)444:51
(x)
344g
(a). scan) *
(alca
IanTI
LOW
9910
9C9aC
Lo9Tt
90agn
610005 a
9
9
La,
Ln raC
Ober
9a05o
Mar
StiC69te
LLot64111
**
29C
LooSo
165 t.
oil per 100 grouts' boas dry istsis)
A
1
2
3
14
0
0
0.145
0.42
1.76
F.Total
5.66
Mean 0.147
0.38
0.67
0.60
2.20
0.1,0
0.33
0.49
0.148
1.89
041
0.56
0.50
0.148
2.05
6.50
0.69
0.59
0.83
0.65
2.56
thit7
0.55
0.39
0.148
0.514
0.55
0.60
0.72
0.52
2.39
0.66
0.43
0446
2 Alt
0.148
0.66
0.57
0.57
2.28
6.71
0.56
0.145
0.54
048
0.67
2414
6.62
0.55
6)0'02
0.67
0.614
2.58
0.48
0.42
0.148
0.52
1.90
0.61
0.147
1.89
2.20
2.00
2.14
3.23
25.149
0.53
a
C
srt
975 met
099
9
crt a9'9
Owl
Cet
't
SS
11,*9
Ma&
'9
Z£'9
Jo a
Armiros-tramoswi
Wvoloo
96eLo floa00°
C
9
LZ
9C
Lit
/woo
69
3400 i
1 reaching WOO 274
5400 ppm reaching
812
27,6
6100 9pm Willi flower 23.0
SI
185.2
182.8
2795
20,0
19.4
12.7
26.8
16.4
1762 141.0
5339.96
14907 58 4637 02 298902
960
990 99.7
81.5
75.7
6834
75222
750
Source of variation
Treatments
UP*.
Error
Total
6
3
18
27
178.709
86.3292 P < 10 *
59.56911
32.6509
* ir
2.614 reclaims 2.66
5%
WOO sp
uo isaktimu
891:
411
03 001 Rild '44.941 I*9 c
00 otod 9.11 q; eti 001
1014 t-ot
1.01
'5
005 'Add
31-0T 61;
09 pa
Iimpewcid 411:Plant L'11
'L cos
TiTvrtretwdet 21t-Tasmtou Ssot
Is a
Is ri tr9
65
9*
5'01
III
$*5
6*$
1.°
96 56
Al
0"9
C'$
L 1.
9'6
()on
zon
114
6C L
1165
6'6
L'Ot
1.19
VT9
90
595
9.1.,1Z
$9CC9 W095 10*(29 65'915 999ee6 soxaog
O uowirraweA
Bizeuroon sumirortdou moan
Troy
9
VT
La
Tit notts6t
** tC6 nest
2
3 500 pp at 114
37
It5
Is 100 ppm at 10.12 ft.
5 500 ppm at 10-12 ft.
32
6 100 pm at lit bar,
31
7 500 ppm at lit bum
30
2117
8859
311 ha
1e6
32
311
29
31
237
214
31
31
2/42
37
31
54
9382
123
980
13900
314
35
Source of variation
Treatimsvbs
Replications
Error
Total
6
3
18
27
99.9
700.0
5.55
(ixt.) in the Gibberellie-aeld.
Treat, 1 2 th
Total 1
Rep.II
2 3
14
Total 1
Check 141
71
0 0 0 0 0
10' 6
.1
5 e
2 -20
0
U
10
15
0
114
0
17
10
4 9 7 6 26 10 13 13 10 46
57
6
8
7
Total 10 16 12 8 46.
23.
38 27 27 113 21
2
141 14
0 15
214 143 8 18 8 0 34 36
7'
9 8 23 37 77 14 14 17 10 55 31
10' 11 9 16 24 60 13 19 15 13 60 33
Total
214
17 54 85 180 35 51 40 23
19
100
Re .111
2 ,3
14
16 0
20 7 7
17
6 11
53 13 18
0 10
9
17 32 1.2
23 15 31
140
37 52
BaNIT
Total 1 2 3
22
42
41
105
55
72
102
229
7
114 lo
11
39
0 5
10 9
9 3
19 17
23
10
10
8
7 a
I 13
28 3.6
52
0
7
9
3 41
71
5 7 25 23
21 8 23 17
60
69
0 II 0 0
9 3.5
11
4 16
0 35 25
10' 17 13 29 24 83 15
111
23 18 70 27
Total 43 28 77 64 212 24 33 34 18 109 68
41
7' lit 19 17 8 58
18 8 12 1.6
514
0 15 7 3
3 9 12 16
25
140
0
20
10' 12 10 12 19 53 10 9 10 13
142
15
Total 44
37 la h3
145 33
33 PO a
109 35
5 41 0 0 13 0 13 0 7 25 20 52 0
7' 12 9 18 7
46 19 16 21 30 86 18
10'
27 31 13 9 60 12 10 25 23 70 20
Total 39 20 44 16 119 31 33 71 73 208 38
8
17
27
52
72
0 0
13 7
26 la
39 25
9
12
0 0
15
114
16
$ 11 -011
33
22
1.7
10 6
23 20
24 24
57 50
24 11 0
62 19 8
98 33 10
1814
63 18
9
0 0
59 0 15
60 21
114
In
21
149
83
8i;
0 0
7 13
13 23
217 20 36
0 0
9 18
8 11
17 29
5
19 19
21
214 igt ILI 1,38
0 0 0
11 18
4.9
16 16 41
27
Total
34 117
632
Side n h (in.) in the Gibberellic -acid Trial on Fuggles, 1958 -- pouts
Rep.I
Rep.II
Rep.III
Reptr
Treat. 12 3
4 Total 1 2 3 4
.
Total 1 2 3 4 Total 1 2 3 k
T
6 41
7'
101
0 0 0 (0)
4
8
0
4 13
(7) 28
10 12 (10) 40
19 17 0 14
8 14 13 12
14 19 12 12
50
47
57
36 0 6 0
13 8 11 7
18 13 10 21
42
39
52
0 15 6 0
13 11 10 9
10 9 14
8
Total 12 14 25 17 68
41 50 25
38 154
7 4'
7'
101
14 8
28
27
6 0
14 21
8
28
71
17 13 26
73
Total 69 39
40 24 172
0 11 0 23
22 17 19 14
14 19 22 17
34
72
72
36 47 41 54 178
67 21 27 18 133
0 24 0- 0
17 21 17
27 27 17
19
21
24
74
92
44 72
34 40 190
23 35 30 17
0 0 0 7
15 20 12
14'
24 22 6 13
7
61
65
39 42 18
34 133
T
41 171 293 257 962
201 285 267 265 1018 373 346 231 236 1186 196 216 188 225 825
4351
1949
5277
5235
2871
4221
4735
4743
9261
7136
3735
4258
3646
3230
2302
_3621
10887 14831
4815 14355
155054 12021 12295
6349 11673 156964
24159
19218
8769
9166
214584
7260
5580
8251
Alpbameold Costomt, Oibberellia ANSA (Mg)
(%o(.astalo Detre 001021ailirla Illathad) a tral
110 ppa 449 high 2
1
Miati
4.06
441 448
3.65
3.94
4.Cts
4.39
500 Xs 3 4.38
642 4.24
110 PM 10.
500 P a w
4
5
4.014
4.60
4414
4.17
14.41
4.28
4.36
3.62
100 ppm at burr 6 3.64
3.51
3.62
3.64
SOO ppm 7
240 2.69
2.64
2.63
17.32
16.87
1645
14.41
10.76
16.23
4.e6
1642 4.0 0
4.33
4.12
4.1,
3.60
2.69
26.39
26,79 27.70
27.48
7148,36 3.87
Treat.
Esp.
Error
Total
Analysl.o of Variances Gibberollia Acrid: .0(-021A.
SS
7.8021
.1578
1.7890
9.7489
6
3
18
27
1.3003
.0526
.09939
13.0828
4141.
A.S.
0 Sew« hill
D Mod. hill
Pled 14/25
SI
23.6
22
23.2
16.2
20.2
21.4
26.4
234
2140
17.1
25.2
17.6
28,6 21.9
23.7
22 26,0 25.8
24
28,9 22.9
21.8
15.9
32.1
16.6
19.6
181:4 830
19.3
1734 800
19.9
30.5
25.6
31.1
1940 !1©
11}.4 117.5
1014.9
123.6
933.1
2656.96
2800.37
2293.31
2845.26
2552.23
3640.68
2463.84
17h,50141
3170.02.
Analysis of Variance
Source of Variation
DE
Treatment 4
Repo
Error
Total
28
7
39
SS
45.75
11.44
3.12.02
16.00
498.01
17..79
655.78
150 Mat
26.0 112
23.6 108
23.2
98.
211.0 1090
17.6 89.7
38.1 32.5
4440 33.7
53.3 35.5
5440 3514
46.1 43.2
22.4 9344
22.2 99.9
20.2 209.0
23.T113.4
24051110
35.4 28.3 19f
.5 23.4 164 724
.5 33.4 ask 980
.5 31.6 174 89.2
310 21.9 98.0
221,5
183.4
114.4
519.3
235.5
180.8
117.5
533.8
.6
155.3
latiot
1465.8
9909.99
2656.96
1126901
6777.50
5429745
2800.37
6607.24
57389094
8557.60
2293411
4905.89
441 8
Rep419
A Se
8 mod#4/11
C seve4/11 g 26.4
32.9
39.4
47.3
47.5
42.4 20.1
304 26.4
33.5 23.4
404 25.2
27.4 23,7
88.
904
96.3
112.8
98.6
sic
Bop."'
R.P.VI
32.3 140.6 23.8
51.4 34.1 22.4
45.4 37.1 28.9
49,4 32.5 15.9
33.4 29.8 19.9
96.9
37.4 36.3 21.9
95.6
107.9
44.0
310 26.01.7
111,4 45.3
37.7
22.9 105.9
97.8
56.9
)9.9
434 434
143.9
32.1
1200
30.5 117.4
193.5
1744
1124
486.8
211.9
174.3
U0.9
4974
226.6
189.5
133.4
549.5
7825.27
2845.26
6249.67
47758.74
9302.33
2552.23
6148.15
10473046
49912,43
3640,68
.
7263.09
61095.03
A No Pruning
B Mod
C Swv.
D Mod.4
Mod.4/2
Rep.VIII
45.7 45.6 19.8
54.1 38.7 25.8
48.4 41.6 21.8
60.5 43.1 16.6
414.45.9 25.6
111.1
40,5 38.1 29.0 107,6
118.6
53.3 37,4 214.6 115,3
111,8
120.2
41,7 38,4 19.6
99,7
41.0 46.0 19.3 1014
1134
9.4.1 50.0 31.1 13205
Treatment
Total
813.5
014.5
831.5
879,5
850.6
Si 250.6
2169
109.6
575.1
228.7
209.9
12306
562.2
4189.6
SI2
12773.72
2463.84
9272.03
10609'23
66216.01
3170,02
8940.93
63838,60
3
5.36
33:9
33.9
34.6
36.6
35.4
Error b
6
70
610.329
564.789
11.557,29115 .*
76.2911 ** soak
12,2254579
Treatment z Tears 2-wy table
A
B C I)
IL
1956
1957
1958
EL
SI2
3094
311.9
191.7
361.1
266.2
187.2
361.9
288.2
18144
397.h
308.2
173.9
3143.6
308.1
198.9
813.5
81144 831.5
879.5
850.6
230,068.51
236.299.49
2146,936.81
283,155.21
252,5147.78
Treatments
Reps.
Error
Total
Analysis of variances sidearm lengths 1958
14
3
232
239
1,476
779
13,238
114,993
MS
269.0 **
259.7
56.6
Total
1,773.9
1,1482.6
933.1
4.189.6
A No pruning
C
Mod. pruning
Ser. Pruning
D Nod. pruning 14A8
It Mod. pruning
14/25
SX
Em2
31
19
23
27
28
23
33
25
27
24
21
30 32
118
2890
128
33614
132
35145
118
2870
'14
Source of variation
Treatments
Replications
Irror
Total
14
3
12
19
69.7
30.4
271.1
3712
17.42
10.13
22.59
Sidearm Length (in.) in the Time and Severity of Pruning Trial en niggles, 3158.
Rep.I
Treat.
1 2 3 4 Total
A
Rep.I/
2 3 4
141
32 28 33 3 96
7' 4 2 13 3 22
0 0
8
0 10
0 7 0
10' 12 5 16 12
145
12 6 7 7
Total
1 2 3
10 19 37 22
15
32
18 13 5
19 14 9
4
8
9
Total
93
51
2
Rpar
3 4 T
O 39 30 23
114
16
114
9
28 33
23 9
Total 48 35 62 18 163 20 6 14 17
57
56 64 36
32 188
B 141
7'
0
114
2 12
5 11
7 6
28
29
0
14
6 0
9 8 6 8
10' 10
114
9 9
142
12 9 8 9
Total 15 39 18 27 99
21 21 20 17
10
31
38
79
8
9
0 11 0
7
5 12
5
17 23 3.7
39
17
15
71
50
37 la.
128
G itt 13 7 0
7' 9 7
5 12
0.
20 0 7 0 0
33
10' 12 8 7 11 38
0 11
4 4
7 9
7
19
39
8
0 13
10 10 11
9 14 13
27 24 37
0
10
21
39
46
D
Total
314
22 12 23 91
11 30 11 13
141
0 6 0 0 6 0
7' 5 7 16 6 34 8 17
10' 11 9 12 8 40 9 33
0 16
114
9 12
11 33
Total 16 22
28
114
80
65
42
146
17 30 36 39 122
E 41
7'
10'
0
17
15
0
8
0
7
O
O
9
0
25
39
0
6 7
0 20
0 20
11 32 36
10 11 17
21 59
Total
32 12 11
9 614
16 18 48
33 115
SI 1145 1,30131 91 497
314
9
0
9 28
12 9
18
48
50 21
143
70
0 0 0 0
7 9 6 5
8
114 u.
7
15 23 17 12
106
108
35
141
184
0
27
40
67
85 105 129 119 438 165 155 150 203 673 sx2
2363
1734
2187
869 835
1139
3567
1485 2835
2727
2196
5189
27 68 (fr 37 229
O 14
7
12 9 8
16 13 14 11
j
35
28 26 29 20
O
9
16
0 24 0
11 10
23 22
25 48 22
23
3.0
103
84
314 no
56
38 49 22 57 166
0 o 0 0
13 13 20 32
14 12 12 12
135 193 198 VA
3747
1863 3
© 32
1b,
2596
58
50
108
656
, erg 4965 5217
3858 1859
55147
1507
2601
14317
3357
41824
6879 5092
6171 11433
101309 5951 6075
391/ 9142 101530
&eta Mg
181
D
E
603
3.62
3.72
4.21
4.08
00(mosaid, D4445:$ celeriac rio mothod
Pussies
!Mt
4.34
3.85
3.26
3.96
3.96
4.18
444
3.68
3.51.
4.22
Ba41
4.04
14.62
3.40
3.96
3.60
kiiit
1.6.59
16.63
1606
15.61
15.86
445
4.16
3.52
3.90
3.96
19.63
19.62
78.75
3.94
Treat.
Rep.
Error
Total
Analysis; of Variances
09194
06089
.34294
2.49577
4
3
32
19 cc
MS
0.2729
0.0203
0.1119
2.431
1.3.
M.3.
mid
8.145
oil 0.85
rug. a-acid 5.20
oil, 1.56
B.Q.
a-acid 9.43
oil 2.08
a-acid 6.17
oil 1.59
Hall a-acid 5.92
oil 1.49
103 -I a-acid 3.41
oil 0.92
a-acid 5.61
oil 1.40
108 -I **acid 8.56
oil 1.85
109-1 a-acid 6.93
oil 0.88
112 -1 a-acid 7.05
oil 1.59
123-I a-acid oil
124-1 a-acid oil
6,92
1.1?
6.82
1.32
127-1 a-acid 6.28
oil
128.1 a-acid 10,30 oil 1.44
129 -I a-acid 6.25
oil 1,42
1.32 -I a-acid oil
3.10
0.31
54 a-acid 2.85
oil 1.43
7.72
1.13
3.3?
0.33
MN&
.
3.77
0.90
5.18
0.95
8.14
1.58
6.43
0.83
9.96
1.20
8.84
042
$.58
1.21
NOS
8.39
1.05
8.33
1.21
6.21
1.54
144-I
3.72
0.38
ha%
1.20
8
4
6
7
1
Fugg.
ii.0.
Bull.
1031
107 -I
1081
109-1
2.67
2.78
2.59
2.43
3.36
3.64
3.39
3.48
4.28
4.67
4.28
4.39
5.56
5.94
5.74
5.38
5.78
.....,
7.59
7.44
7.147
7.88
7.84
7.65
7.40
4.43
4.64
4.61
4.28
5411
5.06
4.81
5.02
5.68
5.49
5006
5.45
6.24
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
0
3
6
10
.
..
.
._
..
2.68
2.42
1.92
2.21
3.85
3.95
3.63
3.69
3.67
3.76
3.34
3.57
3.78
3.67
3.50
3.12
7.10
5.87
5.28
5.20
5.80
4.45
3009
3.99
505%
5.35
5.43
5042
5.28
54.;2
5.85
34100
2.72
2.74
2.56
14.10
4.25
4.01
3,86
6.42
5.10
4.63
4.18
..
....
.00
3.43
3.76
3.11
3.40
5.09
5.37
5.06
4.79
5..73
5.85
5.76
5.42
5.64
5.57
5.33
8.88
8.44
8.86
8.32
..
....
....
...
..
7.32
7.67
6.86
6.99
3.06
2.65
2.72
2.45
3.92
4.21
3.73
608
3.75
3.94
3.60
3.65
4.94
3.83
3.71
3.52
6,10
4.68
4.29
4.12
4.50
5.09
4.47
4.41
.
5.00
5.10
14,86
4.83
7.68
7.87
7.14
6.78
5.34
5.39
5.25
5.39
8,04
7.93
7.80
7.52
7,60
7.76
7.26
7.20
3.29
341
3.00
2.88
4.28
4.10
3.88
3.73
6.30
5.40
4.99
4.72
5.35
4.77
4.23
4.88
3.47
3.68
3.26
3.39
3.97
4.3.6
3.85
3.87
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"C8
* Of
F.O.
nexem City, Washington
Dear Mr. Dwinal tour letter of March reviewed.
In accordance istratoris Mies has been advi tie* of the National Hop Research
C. Culbertson has been requirements, the Admin..
of the etleetivies and ergenisa.
Committee.
WS believe that the Committee will serve a very useful function and are pleased to approve the appointments of Mr. Stanley Brooks as representative for the U. S. Department of Agriculture and Dr. Chester
Horner as representatives for the state of 'Oregon.
For the Committee to accomplish the work of evaluating research results end preparing information for release to growers we agree that meetings should be held not lees than two and not more than three times each year.
Your organisation has taken, a mast progressive step in sponsoring the organization of a National Hop Research Committee.
The purpose of all State and Federal research on agricultural problems is to reduce production hazards, increase efficiency of crop production, and improve the quality and farm value of crops produced.
The job is not finished until research result3 are tads available to growers.
It is our hope that the new Committee will function effectively in helping to bridge the gap between research workers and growers.
Sincerely yours,
L. M. Fultz, Acting hief
Oilseed and Industrial
Crops Research Branch cc: M. W. Parker
J. O. Culbertson
S. N. Brooks
C. E. Horner
121?; ass
183
..COPTes
lanwert Norris Chief 14004001014 211011r04 and Asgard* Staff, Oral* itAAMInistraWre
ASS
L. S. Pelts, hating Obi41/01340041 awl
ImSnotrial Crepe Mosearch Bra m&
Organisation and purpose of National Sep Researeh Commit
At the roiliest of the Sop &ewors sf Anerisa, Ins., a national organism' hopgrowors, a National Sop Beseareh Committee has boon formed. The objeotives of the eommittee will hes (1) to provide for correlation of research aottvities and for exchange of rOseareh information among speeialists invpstigating hop production problems in the four hop-greying states; and (1) to provide for evaluation of research results and for dissemination of current findings among growers.
As new planned, the sommittee will be a continuing body until, t ted tor agreement among the sponsoring agency and members of the ttee.
Meetings of the National Hop Research Committees will be held not less than two nor wore than throe times per year.
For 1958 the menherthip of the Committee will be as follows*
Naas and Organisation Representing
S. N. Brooks Chairman of Committee
Research Agninemist, Crops lessareh
Division, ARS
B.S. Department of Agriculture
C. B. Skotland,. Spore
Plant Pathologist of the Committee*
Washington Agricultural Experiment Station
State of Washington
Hotter, AgenPathologitt)
Greps;HaCearchtDivisioni,ARS-anU
Oregon Agr icultural Eiperiment Station
State of Oregon
W. G. Golden Jr., Farm Advisor
California Agricultural Extension Service State of California
* R. R. Romanke* Plant Pathologist
Idaho Agricultural Experiment Station State of Idaho
* Omitted on original list through what is presumsd to be an oversight (Sip!).
ting of National Ho k Hotel, Yakima. Wishiagtes,
S. N. Brooks, Chai
USDA Oregon State
C. S. Horner, USDA and Oregon State
R4 R. Romanko, Idaho Agri. Experiment
W. G. Golden, Farm Advisor, Sacramento County, California
C. B. Skotland, Secretary, Washington Irrigation
Experiment Station, Presser of the conclusions reached,
1.
be one day before the Hop Growers
1 convention.
2.
Before this meeting an exchange of summary research reports.
Reports to be summarized as quickly as possible in the fall.
(This has been dons for the past season's work.)
During this meeting a combined state report will be drawn up based on the summary reports and will be submitted to the
Hop Growers of America, Inc.
3, Ross Dwinell, Executive Secretary of the Hop Growers of
America, Inc., is to contact each worker for information for his bulletin.
1.
Each member of the committee discussed his present program under investigation.
The committee =aborts agreed that it would be advisable for each research worker to observe work in progress at other stations, contingent on the availability of travel funds.
184
185
Dobta Romanke, Asst. Plant Pathologist, Idaho h ISpata.,Parsea, Ida*
B. N. Brooks, Research Agronomist, USDA and Oregon State College, Corvallis
C.
$. Horner, Plant Pathologist, USDA and Oregon State College, Corvallis
C. B. Nelsen, Agronomist, Washington State College, US, Prosser, Mesh.
G. B. Skotland, Asst. Plant Pathologist, Wash. State College, IRS, Prosser
Minutest
A short discassion was held on what should report of this committee to the "Hop Growers of suggestions were made:
1. This was to be a progress type
2* Reports should include description of the work and summary and interpretation of results.
Information to be included would be at the discretion of the individual researcher.
No recommendations to be included in this report since in most instances might have to be modified after further work.
List the publications for the current year from respective state.
Reports are to be handled as follows:
1.
Get report cleared through proper administrative charnaels
4 Send three copies (at least) to the secretary of the "National
Hop Research Committee."
The secretary is to combine am and forward them to the secretary of the "Hop Growers of America" as the annual report of the "National Hop Research Committee*" One copy is to be sent to chairman of NHRC, one other to be retained in secretarial files.