199 of (oAES 36, cReS) HOP INVESTIGATIONS

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199

ANNUAL REPORT of

HOP INVESTIGATIONS

(oAES 36, cReS)

Distribution of Copies

1. S.N.Brooks,

Farm Crops Dept., O.S.C.

2, Industrial Crops

Section, A.P.S.

3. Director, O.S.C.

Agri, Expt. Sta.

h. C.E.Horner, Bot.

and Plant Path,

Dept. O.S.C.

Chem Dept., O.S.C.

5. S.T.Likens, Agr.

6, Industrial GYops Section, A.R.S.

'

Farm Crops Dept., O.S.C.

Vi le baiter S. Sarney

Sa t Likens, Chest

26

27

Oft (

36) T

HOP DISE

C. 1. Horner

ON OF FUNGICIDES AS DUSTS OR S

CONTROL.

]$S8 Field Trials with Streptomycin

Streptomycin Absorption by Field Treated Hops

Soil Treatment for Root Rot Control

S

CRe5-3 (OAES 36) THE usibLOPMENT OF PIE

TECHNIWES RELATIVE TO

INVESTIGATIONS ON HOPS.

it

USE AND L

DISEASE A

CATIONS 1IAT

AND QUALITY IN HOPS

52

53

) CHEM

OF HOPS*

S. T. Likens

STIGATIONS RELATIVE TO THE

A. Evaluation of Strobiless Alpha and Beta Acids

B. Evaluation of Strobilost Hop Oils

C. Evaluation orStrobiless Storage

ATION

67

67

72

76

APPISIDIX

# Planting Plan

Utica Testing, Nenorandan of Understanding

...station Testing, Preesdares for Cream hinieal Stripping and Sastering Trial s IrriptIon.440441ity:Seprtsant s o f t itterellis Acid on ?sales

Sorority of Pruning Study rags Data Levis Brows Sondless Yard

Stara. Data @silty Trial, 195748

National Bsp Rosearoh Conmitte*

957-0

fir.

the VIDA ixperiment tie* at Prosser, Washington of California. For sane in reviewing, descriptions, senmeries and sammariming data are ineleded in the fore part of the report by specific line project.

Additional data uhioh are important enough to include as a orator of permanent record are included in an appendix at the end of the report.

Knee lame of the line projects are eomducted eeope personnel located at Oregon State College, more than one investigator may have work reported in one line project report. An attempt hap been made to give each investigator full credit for his contribution to the year's work.

The work reported in this report is directed along five lines involving studies on hop culture, breeding, disease and quality investigations.

The studies on hop culture and breeding are cooperative among the agronomist, the chemist and the plant pathologist. The studies on disease control are

Chemical studies are 000perative between conducted by the plant pathologist the Chemist and the agronomist on certain phases, and other phases are con.

ducted independently by the Chemist.

A fifth type of investigation inoludes studies of field, greenhouse and laboratory techniques relative to the breeding, disease and agronomic lines of endeavor. Any OM of the project leaders may have work reported under this line project from time to time.

In addition to the support given to this work by public agencies, certain annual grants of funds have been contributed by the Brewing

Industries

Research Institute for partial support of the work in Oregon. Begiming in

1959, such support will come from the United Brewers Foundation instead,

point out the

*web personnel and of Mario., Ins. last 'interim Salm, On as liaison between the rooms* groups in the growers The eemmittee was to alt as a in bringing research results to the grower and as a body to work in that different areas to eliminate us-noodod duplication

Tho ionsitteo was named the National BO, Research Committee and was mode up as follows,

Name and organisation

3. N. Brooks Chairman

B. BvDepartmont of Agriculture

C. B. Skotland

Plant Path., W

C. E. Homier

Agent (Plant Pat.), ORD ARS and Ore. Agri°. Expt. Sta.

W. G. Golden, Jr.

Farm Advisor, Cabo. Agric. Rzt.

R. R. Romanic,

Plant Path., Idaho Agri°. Expt. Sta.

Washington

Oregon ornia

This committos met twice during 1955 and will meet again

Sacramento, California on Feb. 4, 1959.

On February 5, 1959 it will present current research findings to the Bop Growers of America, Inc. at their annual convention.

Bop production traditionally has been subject to wide variation in total acreage, total producrtion and center of production.

The hop industry suffered a severe recession in the early 19504 due to low prices.

Since 1956 there has been considerable improvement in this situationl and the industry is ones again on a sound basis. A oontinuation of expansion could however,

n

3,951 27,700 33,1 00 1,473 lrket die hh9 149,514 40,135 h5,1s0

T train

Division, USDA

7 ly

Aug.

Sept*

534

54.6

73.0

4

864

75.4,

!early

total

!early

scan 64.1.

63.6

55.8

62.8

69.2

73.3

80.9

81.5

76.7

366

40.8

46.7

.5

55.9

48.6

434

38.0

35.,

.3

.3

37.6

404

45.2

494

52

4

524

494

14 40 las e

44.3

4,041 7

59

63.6

70.3

62

traripitatiat

7r.

avg

3.114

2.01

3.54

3

.7

51.5

57.1

61 .8

46

4

67 4

63.0

8.15

8008

I+55

3,66

1.12

2.91

.02

2.4a

6

3.67

242

1.71

1.44

38.42

38.39

42.7

53.6

Month

Reldnuld.d. Evepora.

SOAK 0) tlen (ln.)

6yrs

1958 Avg.

tr

Noptly.

cloudy cloudy

Me.

rainy

27 yr.

18 18 18 18

1958 Arg 1958 yr. 1958 yr. 1958 yr. 1958 yr.

Avg.

Avg.

Avg.

Avg.

A velGoity

(MPH)

6 yr.

1956 Avg,

1958

Apr.

May

81.4 81.2

1.105 2.684

7 8.8

19 .3

7646764 5.201 4.103

12 2.0.0

12

1211.4

June 80.0 76.3

4.514 4.679

9 8.3

July 69.6 70.5

8.287 6.551

22 17.7

13 124

9 10.2

Aug* 65.4 73.2

8.313 5.834

17 16.0

Sept.

5 8.8

761A0.2

4.795 3.865

6 14.4

13 9.1

3 9.0

22 13.8

2.37

244

7 us

9 10.5

495 1422

7 9.1

14 9.2

1.06 1.27

1 30 1 2.7

1.75 1.52

2 5.,9

2 3.0

1.80 1.55

5 54

9 5.6

2.15 1.68

Yearly total

TearlY74.9 76.3

mean

91

73 75.2

71 660

25 40.5

57 44.8

1.68

1.56

order to data are all than being separa and involves the three to more meaningful, agronomic hemical and disease byevaluation trial within this line project rather three sections of the report.

Genetic diversity for the breeding program consixte four widely grown varieties and approximately 75 introductions of which only a few remain.

Many of them are new represented only by first or later generation progenn and some have been discarded entirely.

gone of the varieties or introductions have exhibited immerity to downy mildew.

Some of them possess acceptable quality and agronomic characteristics, but none of them are sufficiently satisfactory in all important features that improvement through breeding is not desirable.

Due to resent reports from Wye College in England that varieties of

American parentage exhibit resistant to downy mildew crown infection, a now approach was initiated into the breeding program.

C. E. Horner collected living specimen* of the wild hop native to Utah last spring.

Clones of these plants were established in the breeding block and will be used as breeding MRS terial in future years.

Accession numbers will be assigned to the clones when it has been determined haw many of them will live.

Since its inception, the hop breeding program has been confined generally to inter varietal crossing involving both domestic and foreign varieties.

A modified recurrent selection scheme has been in effect since 191s8.

Selection has been based upon resistance to disease, primarily downy mildew,

planted

Trials were placed in the o & an. ,ttea pt to duplicate of the egomania tion phases of this project were expanded during the year.

each of 1034, 1014 1034,

1$..1 Ind 139 were

trials by °. I. Nelson (Prosser) in the Taking VaLlay.

Dare Streams of Hibton, Laverne Aires of T nigh and

Lawrence Brullotte of Memo City, Washington.

Approximately 100 hills each of 112 1284 135 and were planted in each of three trials in the Willamette Valley.

Trials were placed with Ray Kerr of Salem and Ed Crosby and Louis Schwaben*? of Woodburn,

Oregon.

Clive Cook and Hollis Manley, County Extension Agents, cooperated and will continue to cooperate in this prograa.

Plans are being made to establish one more off - station trial in this area.

During the winter of 195148, hills of each of the eight lines represented in the Oregon and Washington off-station testing program were planted en the Ranch of Peter Bradley of Elk Grove, Califernia.

Due to winter flooding conditions Shortly after planting, considerable stand loss was experienced.

Additional cuttings will be sent in February 1959 to W. G.

Colden

Farm Advisor at Sacramento, who is cooperating in the program.

In December 1958 10 cuttings of each of the afore-renti sent to R. R. Romanko at the Parma Branch Experinent Station in Idaho.

plant them in a cooperators yard and supervise their future evaluation.

Lines which are being released to growers are done do under an informal memorandum of understanding.

A copy of the memorandum can be found in the Appendix of this report.

In the appendix also is a copy of the procedures

.5

had resulted fret what wee presumably

Cross between Late Gloaters and a sex-reverted Leto Cluster MU total of 59 progenies available tOr study in 195%

The growing season at Corvallis was unusaally hot in 1958 and was particularly hard to the breeding pi ogrwa Maar of the foils flowering branches were blasted or otherwise kondered non-functional under the parchment bags. As a result only about 15% of the crosses mods produced seed.

This is about one.third as many as are good in the average year.

Group Is 54 crosses were attempted among resistant and susceptible male and female plants in order to obtain seedling progenies on the crosses used in a downy mildew inheritance study set out last year.

Only sight of these crosses set seed, and the whole group will be re-made in 1959.

It will be desirable to have seedling reaction on these progenies as well as mature plant reaction.

Group III Six (grosses were repeated (25 attempted) involving parents that had produced downy mildew resistant seedlings several of which had gone into the 1957 nursery or elss bad had an insufficient progeny test in 1957,

These crosses were the same as the Group II crosses in 1957. They were 58001, 58010

58012, 58034, 58053 and 58057.

Group ITIs 16 crosses were made (104 attempted) between female plants which had produced several resistant seedlings each from open-pollination in 1956 and 1957 and males which had resistant progsrq in either 1956 or 1957. These crosses wore,

58002

58013

58032

58033

58042

58043

58044

58045

58047

58048

58049

58051

crosses, obtained

5$036 a prover test en will be salso.

197 in 1959.

se plants along with 219.4 should be tested further

Croup Ts Open- pollinated seed was dollected several relatively new females in the breading block to provide a mildew evaluation progeny test On the basis of this test these nee lines will be saved or discarded from the breeding program.

The lines were!

104-1

114.3

2154

301.1

3013

No OP

302.1

302.3

306.1

306.2

306.3

a obtained from 504.1, and this line will have to be tested another year.

Open.polli

obtained from 18-8 (C 19113) which is the same as 128-I now undergoing off-station testing as a seedless or sterile high.yieldingo high analysis lino.

This genotype set seed this year rather prolifically (for it).

It will be of interest to see what its progeny look lila

Open.pollinated mead of Leta Clusters was obtained from Robert the Parma Branch Experiment Station in Idaho. This seed resulted

fawn polliattice by presumably iscrormirsed Leto Gluing.

for mcgAgation for Walt reastien.

121-2 and 1 s23,40 were made in 311 (I 3S001, Brown* of starting a bask Grossing program current parent. Mae lines 1212 (G 19062 10, WO

Itrowers

190140

(C 19122 N) are vigorous, mildew resister& lines. Those lines will be in backeroowing Programa with Brewers Gold, Late said lerly Clusters lallertma and Backs in an attempt to incorporate mildew resistance and vigor into lines resembling the domestic and foreign varieties in quality. The seeds were put in moist sand and placed in storage at Xer. on January 5, 1959 are they will remain until February 16 at which time they will be renaved to the greenhouse for germination. The resulting seedlings will be subjected to downy mildew screening and selection.

5110011

106

) Q 500,1 s 32.4 t 313

3

C

5346 x

) C 19034 x 321.2 (525) C 3.9062 X

50005 106 (506) 0 19032 x 2214 (1s25.1) C 51101

X

58006* 106 (506) 0 19032 z 3174.1,, 2 (317) C 1904 x

0007 41 106 (506) C 19032 z 321.a. (325) c 19049 x

58006 * 106 (506) C 19032 x 121.1, 2 (225)

C 19040 N

58009 * 106 (506) C 19032 x 52141 5 (126)

C 19010 M

58010 108 (506) C 19033 x 124.3 C 19183 14

58011 114.3 (5144) C 52020

x 0.P.

58012

58013

203 (103) c 19022 x 1418.1, 2 (219)

1 19006 M

203 (403) C 19022 x 119.S-0 19180

7

580111 210 (110) C 19027 x 3174 (318 -2) C 510514 it

58015 * 212 (412) C 19028 x 5214, 5 (126)

C 19010 Di

58016 215..3 (4154) C 52012 x 0.1),

52017

58018

58019

301.1 (301-3) C 50040 x 0.12.

301.3 (3014) c 50019 z 0.P.

302-1 (302.3) C 52001 z 0.1)

58020

58021

58022

58023

58024

58025

58026

58027

302.3 (302.1) C 51065 x 0.1),

306.1 (3136-3) C 51105 x 0.r.

306.2 (306.2) 0 511014 x 0.13,

30603 (306-1) C 53203 x P.P.

307 (307) C 19020 x 2194 (1422.2) c 51061 m

307 (307) C 19020 x 317.4 (3182) C 51054

307 (307) C 19020 x 518.5 (120) C 52041

307 (307) C 19020 x 519.2 (121) C 52042 N

11

58046

58047

58048

58049

58050

58051

58052

58053

58054

58055

58040

58041

58042

58043

58044

58045

58030

58031

58002

58033

58034

58035

58036

58037

58038

58039

(

)

51021 x00,

(311) I 19001

121 -2 (525) C 19062 m

311 (311) I19001z121 -].

2 (225) 0 19040 m

314 (314).0 19076 3: 521A, 5 (126) C 19010

314 (314) C 19076 x lama C 19183 )1

401 (201) c 19022 x 4194 (422.1) 0 51060 m

401 (201) c 19012 x 5214, 5 (126) c 19010 m

402 (202) C 19013 x: 419-5 (222) C 52048 m

404 (204) c 19014 x:317.4 (3182) c 51054 m

404 (204) C 19014 x 419-5 (222) c 52048 m

404 (204) c 19014 x519 -2 (121) C 52042 M

405E03 (205-1) C 54010 x O.P.

414 (214) C 19018 x 518-5 (120) C 52041 m

422 I 56002 x 123.3 C 19182 m

501 -3 (101.1) C 50008 x 320-4, 5 (324) C 1908 11

501.3 (101.1) C 50008 x 417.1, 2 (217) C 19036 m

504-2 (1062) C 54003 x 320,4, 5 (324) C 19048 m

504,2 (1042) C 54003 x 419-1, 2 (221) C 19037 m

504.3 (104-1) C 54002 x O.P.

507-3 (1071) C 54005 x 217.1, 2 (417) C 19050 M

507-3 (107-1) C 54005 x 319-4, 5 (322) c 19046 m

509.1 (109-3) C 5004 x 320.4, 5 (324) C 19048 m

5104 (110 -3) C 52038 x 0.P.

511-3 (111.1) c 53007 x 318-1, 2 (319) C 19043 m

511-3 (111-1) C 53007 x 418.6 (220) C 52047 H

514-2 (114-2) C 53013 x 521-2 (125) C 52045 M

7-8- C 19102 x 517-1, 2 (117) C 19008 H

18.6- C 19113 x O.P.

12

* 0 see repsatsd for DM inhoritanoe study*

eels.

tlisugh a complete motel's not ebtainedit is felt that suffieient

2 x2 and 3 X ) eombinatiers will be smailable to give congiderable infereation on.downy mildew inheritance ?hire will also be various 2 z 3 and tx combinations available ter study immolvingthe following parents (oode Immhers)1

Resistant males are X, T Andl

Susceptible males are R, Sand ?

Resiatant females are Ciand D

Susceptible females are Es F and

Segregation and trap/env distributions of course, can be studied o e cross individually.

The 22 erasses and of the parents were planted in a l0 z 10 triple lattice experiment.

The plots were three hills spaced four feet apart in rows eight feet apart.

One plot of each parent and four plots of each cross were included in each of the three replications.

The main purpose of the triple lattice design was to insure that the plots of each cross were separated as well as possible with respect to each other. This also insures that no parent occurs In the same 1a -plot block more than one time with any other pare

It is believed that this will give mere realistic estimates of genetic and environmental variance when the area occupied by the experiment is taken into consideration.

The variance of the parents will be used to estimate environmental variation.

The triple lattice design is, therefore, mostly for the purpose of field arrangement rather than for statistical analysis.

11 crosses had sufficient progenies that an additional three replications could be sat out which included four 3hill plots per rep. along

15

A trial eon be t

As a re of

Pro of 36 individuals

Prods of 72 individuals have nine clones eash the

U of the oxv

11 of the Gros pt for parents as 0 and represented WI 13 clones total, in six replications.

It is re

However, in view of the area and expense of hops, and in view of the fact that the popalations are extrmeely small.

ed in growing large populations r knows of no ether like experiment, it is considered to be adequate for prelininary data on inheritance in hops.

The results which will become available in future years will be recorded and suimarimod using IBM facilities.

Group II. repeat-- of 1956.3,957 progeny testedArosesst this

Seedling progenies were obtained from 11 of the 19

These aro:wee-mem sews

*57042

57047

57136

57138

57145

57184

57186

57196

Cross No. 57042 had exceptional seedlings.

57218

57215

57256

The performance of the asedlings from these crosses can be obtained in the section of this report dealing with "seedling reaction of 1957 crosses to downy mildew".

11 crrosse s of ro mel

This grOup contained crosses made between openpollinated progeny tested females and proven males. Out of the 79 crosses which had been made the progenies of 38 of them were subjected to the greenhouse downy mildew screening program.

The results of this screening is given in the section on

Only four progenies Imre obtained from the 18 in this group in 1957.

An attempt was made to duplisate this tee few crosses were obtained, and it will be necessary to continue this into next season.

Crosses 57122, 57150, 57157-and 57172 mere tested and 57122

(involving 2018) produced exceptionally good seedlings. Further information is given in the section on "seedling reaction of 1957 crosses to downy mildew."

Groups V and VI: open-. pollination of unproven and mien. females:

Out of the 87 openapollinated seed lots obtained in 1957, seedling progenies were obtained from 6h of them. A decision was made to retain or die., card most of this material on the basis of their seedling pf Ay performances

(mildew reaction and vigor). Following are the results of this test.

202 -3

2044

208.3

211-1

211-2

213.2

215-2

215 .3

222

301.1

301-2

301.3

302.1

302 -3

303-2

3064

304.2

306.1

306.2

306-3

308-3

309.1

309-2

1.114

1114

1134

1134

113-3

Uit.2

1143

115-2

122

2014

201.2

313-1

3134

316.1

316.2

405.1

4054

1405.3

406.1

406.2

406.3

407.2

;:63

..

..

14:94

..

..

1.28

2.28

..

....

--

--

...

--

...

6,58

9.54

0

3.

5.00

2.89

2.35

5.36

4.10

0.99

x.52

....

8.51

4.03

6.58

440

-5.812

0.31

2.50

5.57

5 059

57460

57061.

57061

57065

57075

57079

57085

57087

093

57095

57096

57097

57090

57099

57100

57101

57102

57103

57107

$7108

57109

57112

57113

57116

57124

57125

57139

57140

57158

57159

57163

57264

57165

57166

57167

"

No test

Discarded in 1957

Saved (Late

Discarded

N

Discarded 1957

Saved, used 1958

Discarded

N

Discarded 1957

Saved, used 1958

No test

Saved (Juggle.), no test

No test

Saved, used 1958 test

N w

Discarded 1957

Discarded

MO toot

N

N

N

N

Discarded 1957

N

N

N

1958

N

N

No test

Discarded

Saved, used 1958

of he of susceptibility were observed both among crosses and among within certain °roues. The use general testing procedures were follommi in

1958 as in previous tests cited above.

Pro

Seed from the 1957 crosses was germinated and seedlings planted by

S. N. Brooks. When seedlings were 10-12 nooks old they were heavily inoculated with downy mildew.

Inoeulma consisted of sporangia collected from several varieties and lines to include possible different races of downy mildew, although the existence of races has never been demonstrated tor suspensions of downy mildew spores were prepared by washing infected 'spikes" then filtering to remove the larger particles of soil and plant debris.

Inoculation was accomplished by spraying 20 ml.

of a uniform spore suspension on the undersides of the leaves of seedlings in each flat.

Humid conditions were maintained by covering the inoculated seedlings with cheesecloth saturated frequently with water,

Good infection was obtained and disease developsent was rapid and vere.

Seleotions for resistant plants were made with the assistance of

S. N. Brooks.

The number of seedlings from each cross was recorded.

A record was made of the number of ',atomically infeoted seedlings in each cross, and each cross was rated for mildew severity on a scale of 0 to 3 representing none, light, moderate and severe infection.

Selection of plants to be saved was based on both mildew resistance and seedling vigor and appearance.

table below shows tars deingr Widow,

26466

111

118 crosses

infostiss and

devsloP**4 t differsness eoptibility thin certain creamy The reaction of swains from the 1957 crosses to

7 severity prat

43-0P

44

45-0P

47

48-0?

4560P

50.0P

51

52.0?

54

55.01)

56-op

57-op

60.01)

61-0P

62

64.op

65-0P

68

6

7

75-or

77

79-0P

80 **

81 **

82 **

85.01)

87-op

93-op**

319

323

329

1%

210

219

108

98

85

317

322

208

319

312

107

109

108

215

105

321

104

107

319

320

325

326

312

108

319

322

305

314

7

1

0

1

0

4

2

1

11

3

23

13

9

21

15

8

7

0

11

9

1

1

2

1

3

6

0

10

2

0

11

3.41

2.18

0.0

5.24

4.11

0.92

1.02

3.53

7.2

4.

4.33

6.58

4.81

3.714

1.84

0.92

5.12

6.67

0.31

0

0.93

0.0

2.5

5.57

o.64

0.31

0.92

1.92

0.0

3.13

0.62

0.0

2

1,5

2

2

3

2.5

2.0

2.5

2.5

2

3

3

2

1.0

1.0

1

2

3

3

2

2

3

3

2.5

2.5-s

2

1

3.0

2.5

2.0

3

1

5

10

6

4

3

2

1

4

0

3

1

2

3

3

2

7

2

6

3

6

2

1

2

0

0

3

4

2

7

4

1

8

Based on a scale of 0 disease development.

**Denotes crosses am

2 moderate and produainr many vievor.",,.. as, well as

3 resistant seveme qpwilina-

292

/18

211

99

325'

206

212

303

319

.7,i

211

311

325

107

313

315

315

108

319

210

110

107

214

200

110

289

217

104

104

216

212

325

207.0?

208.0P

210.0P

221-01)

212.0P

2134P

215

216.0P

217-OP

218

219.0P

220.0P

222.0P

225

226.0P

228-OP

229.3P

230

232

233

238 e*

243

245

247

*IF

249

250

251

254

256

257

260.0P

mt.

5

2

5

2

2

6

2

0

3

13

7

3

3

15

1

2

0

2

1

0

0

8

0

2

3

6 o

0

0

0

2

6

8

2

6

4

7

7.27

0

0.93

0.50

0

0

0.92

0

1.91

0.93

2.83

2.46

lt:

2.28

1.42

0.96

4.62

0.93

0.96

1.90

0

0

0

0

062

546

1.91

2.20

1

1 . 3

0.68

1.57

0.95

2.02

1.85

0.97

0

26,166 581

2.22%

2714 a scale of 0 none, development.

light, 2 moderate and 3 - severe rosses producing many vigorous as mall as resistant seedling

2

1

4

1

1

7

6

0

2

3

2

4

1

2

3

1

3

3

5

0

1

3

0

2

2

1

1

2

1

4

2

2

2

3

1.5

1.5

2

2

2

3

1

2

1

1

1

3

2

2

2

1,5

2

2.5

2

1.5

1.5

3

2.0

1.5

2.5

1.5

1.5

1.5

1.5

25

2

2

2.5

1 .5

2 .5

2

2

Yield observation over 2 swims initiate that relatively molested as resistant to-greenhouse sereening twee severe field.

of the seedlings infested in the differous es field roe a

SPA ng pro standpoint resistance to systemic types.

Degree of systemic infection, re

118 crosses tested In 1958.

As parents ducting a high pereentage of suseeptible

0 to 10 to

degree of systemic infeetion should be reduced.

the three years 1956, 1957 and 1958 shows that this is

of the

date for

1956

7,026

31,357

26,166

511

939

531

7.27

2.99

2.22

0 . 24.4

0 . 22.0

0 4 9.5

prosent agronomist holog10 on the basin of vi for

further

testing in the field.

274

jointly by the

and mildew resistance

Onthe basis of 3 years' results it appears oonolusive that green ening with downy mildew is olud natir nest

of the susceptible plants

dewy ands, sereentni preps* roproaeeted Prollentiafat of transp3Aatod in tlos fiold under dal *posing whorls they

fall of 195% At that time they will to seplistad or disearded ws visual inspoetien. The se

stet plants !dill be subsequently inereased for further evaluation

Selsoitions its were saved from 7 827

-plant nursery. All of these salections More female individuals.

Theey will be increased and grown in or

5-hill observation plots. Following is a last of them along with the accession numbers assigned and brief descriptive remarks:

Accession no.

Cross

56014 (X) Good vigor, large Bone, late, med. arose.

C 57001

C 57002

57003

P

5700k

C 57005

C 57006

C

57007

0 57006

G 57009

C 57010

C 57011

C 57G12

56053 (I)

56041

(OP)

56076 (Z)

56143 (OP)

56268 (OP)

56248

(OP)

56171 (or)

56205 (OP)

56211 (OP)

56234 (X)

56112 (OF)

Attractive appearancet lar bright cone, mad. aroma.

Large eons, shatter resistant, net. aroma, early.

GoOd gist of small heavy cones, early, med. aroma.

Heavy set of pals cones, mild,

Seedless, ;mill cones, bright lupulin, med. amnia.

Long sidearms, fairly rich, pleasant aroma,

Vigorous, good sidearms, tight cones, med. aromas

Large round cones hopped down well.

Not much vigor, firm cow:timed. aroma.

Good cone set, some shatter, high resin and oil.

Strong smooth vine, late-, small cones.

passible to obtain *deg** satin disease mereery provides ewe environmental *antral.

'WA for &rosy vdleiew infsotion her. An over mist spray erste* development despite unfavorable la automatic hneidistat is installed car an are where *boat 200 inii

Tits cm be tested im any one mason. The nursery area contains a spattering of mildow.sueceptiblo Tarts and maintenance of mildew.

The nursery area vas

1955. It is anticipated that ma avail for testing in 1959 or 1960

27

To eaialegme and dos ris for each male genotype.

To study phenotypie and genotypic correlations between pair.

of characters.

-UullaiJILEMatels

It is hoped that sufficient data will b obtained during the 1958 and 1959 seasons.

Reasons fo k: asters in o before a breeding pro efficiently. Evaluation of male breeding vales has largely been a matter of progeny testing which is time consuming and laborious.

Often times progeny testing has been incidental to the breeding program with the result that incomplete information is available, Methods are needed to evaluate maim; phenotypically, particularly for quality and yield, if possible, to aid in selecting breeding material.

Mature and extent of evious

The most valuable work along this line has been done in England.

Wormald (J. Agr. Res., 1915) presented a detailed comparison of the variation existing in American and English male hops for floral leaf and vine characteristics.

His paper was largely descriptive having limited data.

No attempt was made to separate genotypic from environmental variation.

Ehara (J. Fac.

Agr. Kya. Univ., 1956) made a detailed descriptive study of the comparative morphology of the hop.

Hama chi (Bel. Brew. Soil', 1955) published in Japanese describing development and differentiation of flowering branches. Most of his

se* dealt with the

*tenet poll eia she in I Pole of males sad hied wide differeness in Leith o pollee ng period. Farrar, News and Whites (Wye

44114 AM4

RIStof 190) tried to resin gland number in IOU hops with resin gontent of related females with no Suesess.

Work at Corvallis; anneal reports, Cie 7)

.demenstrated that mine exist in male fismamms in me

Recent work in Smgland has *hewn similar results but it indioation of the range that might be encountered in a group of satisfactory modification of a method determining a. A and /3 -Add in male flavor* has been developed by S. T. Likens, by which a relatively large number of plant* can be tested feasibly.

20 male lines were planted in a replicated trial of 5 rooliootione of single hill plots in 1956.

The five replications were made by vegetative propagation at the 20 lines and would be expected to be genetically alike in the year that they were planted. These lines were net selected for any particular characteristic, except that one was selected because of extreme earliness, and would be expected to represent a random sample of the male genotypes available at Corvallis.

17 different pedigrees are represented in the group.

Observations were made on the male lines for flowering behavior and branching pattern in the field.

Male flower samples were obtained by bagging the sidearms in parchment bags for 3-4 days while the flowers were shedding pollen. At the end of this time the flowers which had completed their pollen shedding and had abcissed were removed in the bags. They were then dried at low temperature in vacuum and frosen. These samples provided the material for

17

18

19

20

6

7 a

9

10

11

12

13

16

514.31)

X

)

224

317

319

322

320

323

424

X

X Early x Red Vine4

Unknown-5

X Belgian Burring x reggle43

X Late Clustar-5 x !Niggle",

X Tuggle x Tuggle-8

X Elsasser x Faggle-5

Brewers Favourite4

/ 19005 X Late Cluster...5

518

14254 C 51101 ti 308.2 (Fuggle..5 (51.31) x (Landlespfen-5 x (Golden

Cluster

z

Foggle-S) x (Landhopten-8 x (Golden Cluster x Tuggle...3)

521 G 1905! 11 Early Greta x linlaionn4

523

417

524

C 19060 X East Kent Gelding x Bavarian-5

C 19050 14 niggle x ruggle-3

C 19061 14 Late Grape x Fuggle.5

plant from whisk* samples wore availablo.

42Monimontal rot/alto

Partial results me g behavio floral development, eta Resin gland cants were mode On three replica s.

O replicate has been analysed for resin data and three more reps, will be analyzed during the coming months.

The datettoomoleted to date are given in the tollowin tables,

1 14* 4/

2 13* h7

16 4 7

14

$ 13

47

50

66

70

58

51

3

53

55

0

WWW

WOW

17

521

18

523

19

417

20

524

32

3 31

4

5

53

53

51

1933

* 49

7 58

$8

1 19 44

2 21* 49

3 23* h6

74

77

66

51

53

$1

414

39 70 44

516

44 67 53

51 70 55

96

96

94

96

93

9

94

72

93

78

3 27 49 105 55

416

47 72 51

5 18

41 100 47

1 17* 42

2 34 53

5 19

40

40

67

614

62

60 47

44

51

$8

4

44

76 100

70

6

63 ft --

97

3.36

2 24

3 3o

4 12

$ 21

49

49

$1

39

44

72

67

65

63

70

55

51

55

44

47

77

85

65

68

68

97

97

30

28

20

31

23

WM

Wift

WW

OW

..

..

..

..

...

..

..

..

.,-,

..

IOW

WW

WW

WW

25

28

23

25

28

2nd flower berm,

23

18

14

24

26

25

21

25

22

20

19 19 38 19 small plant replant 1958

-------÷

25

WW

56 22

31

)

ai; 23

24 49 25

..

1...

19

--

...

24

..

..

26

..

.

21 leaf flecking small plants leaf flecking leaf flecking

..

12

13 ma

25 small plant

32

..

12

..

21 small plant

27

23

-. Not obtained.

* Not verified by later observation, but probably corrects

No definite break between let and 2nd flowering(overlapping)i

1st and 2nd flowering added together.

3I

106.a

6

223

227

125

221

7

8

324

224

10

31

11 322

12 320

323

11 424

15 518

16 425.1

17 521

18 523

19 417

20 524

Mean

as

ISD(5%)

04

1.0k

1.55

244 1.514

2.8$

0.91

.1.76

2.67

1

0,22

4.58

0.47

3.14

0.69

0.62

1.60

2.22

1.36

0.36

0.72

006011 0.3511 0.95

loll 3.08

620

0043

2.08

0.79

0.35

1.83

2.18

1.71

1.41

0.34

0.71

0.42

1.25

1.22

0.82

1.89

1.05

2.96

2.63

1.16

2.60

1.47

2.54

2.35

0.56

2.74

1.99

1.46

5.28

4.34

2.02

1.33

1.95

3.28

.

y

Data supplied by S. T. Likens

46.7

60.1

29.0

41.8

31.6

10

9.1

22.2

30.7

35.0

18.5

27.1

21.5

19.2

5506

164

29.

41.5

39.5

29.4

36.9

20.6

Nine

Nene

Small gland*

Big an isith few *lauds glands mostly an sepias.

lig flowers and anthers,

°Isar Asada.

small anthers, mill glands

Glands clear and light colored

Fism4 tight flowers

Glands small, nost2y on *opals

Anther famous deep, many glands ruptured.

Large anthers

Large, round glands

Small, light aolarod glands

Moue

Small, firm flavors, large clear glands.

Largo flower, clear glaccls

Anther* flat clear glands

Large anther glands vary in sloe

Name

Photographs showing resin glands on the male flowers

(about 11 x, scale is in millimeters).

Note resin glands on outer surfaces of sepals and in the dorsal furrows on the anthers; glands occur also in the disc from which the stamens arise.

RID

59

d in ear4 lines laths t inmost of the limes Olen ones, was a little surprising.

Heretofore, in the breeding have been eut down by August 1 or shortly after in order to eliminate unnanted pollinatianwhen the bags are removed from the female flowers. Here detailed data will be obtained on pollen shediting periods next year, although differences were apparent in 1958 from the limited observations made.

Significant differences were indicated for resin

The average numbers of glands per flower ranged from 10.5 in 106-$ to 60.1

in 523.

This is a six -fold difference.

Only one replication, of 0( - and r:' -acid a a but a few lines have two replications completed (not reported here).

In b.

general, fairly close agreement between replicates is evident at this time.

In the onezeplicate

-acid ranged from 0.22% to 2.514% * tenmfold difference. Differences of similar magnitude in -acid were also demonstrated. Differences in % total hop -acids (an arbitrary classification) were about eight-fold.

Gorrelation ooefficients were noUlcomputed between resin gland the various hop-acid components.

Scatter diagrams, however, indicated a rather loose positive correlation between gland number and

,

-avid a fairly good positive correlation between gland number

-acid, and a strong positive correlation between gland number and % total hop-acids.

This study will be dicouseed much more thoroughly next year when final and more complete data become available.

Deration of sxp,rimenti aired pre+

This trial is concluded with this summary of the results for 1958 s years.

The 29 entries were planted getative clonal material in plots replicated five times in a randomised block design.

The 27 experimental lines represented a group of selections which made in 1950 Out of crosses made in 1949.

Although most of them were intermediate in maturity there was considerable range for this charaeter tram medium early to late (later than in Late austere)

The trial has been grown under irrigation and has received Eras two to four irrigations per season.

Applications of 111, P, and E fertilisers have been made every year either by ringing the hills or by broadcast application.

Attempts have been made to control insect and disease pests.

The overall cultural program on this trial has been carried out in a manner consistent with good management practices.

Experimental results,

The following table s d data tram 1954 to 1958 data obtained in 1958 as well as information on a portion of these lines is given in the Corvallis-Prosser Yield Trial report and sections A, f 0Ro5-5 report.

3.3

28

27

2

21

22

26

5 103

1114 25,6

311-1 24.0

107.

26.0

139.

L.0

1

1284

26.1

130.1

108-1

124.1

24.1

122 -1

27.2

26.3

27.2

244

254

135-I 25.2

121

24.7

1234 23.8

224

23.8

1384 26.2

20 127 -I 22.1.

10

6

7

14

214

109-1

1044

132-1

23.7

2242

106-1 25,6

102.1

30.1

254

19

18

23

126-1 2793

125.1

28.1

1314 23.7

29

12

3

1

144-1 214.6

1174 24.6

1014 26.0

runlets 29.3

Mean 25.3

CT

LSD (5%)

3

1.8

a.

0

2270

2 1

5

12

3

2

3

0

4

2

7

1

2

0

2

3.

3.

1

1670

11.

3.630

7

2

10

1900 11

0

31

1310 w

3.820

1180

1910

1890

1880

1840

1760

201.0

1920

1750

1720

1570 1660

1000 1610

11490

950

1390

1590

1570

3570

3520 11420

610

1000

1510

11450

920

1200

11400

3.370

0

1860 U420

6 13

510 310

1

I/ Underlined yields greater than that of ruggless basis of LSD

After E. L. LeCier Mean Separation ARS.20.3, May 1957.

for 1034

a

There were also in 1958 t at harvest time such differences are a reflection of since known early lines are usually drier at a common date than known late lines.

It is unfortunate that a satisfactory picking machine is not available at Corvallis with which each line in a trial could be harvested at its maturity rather than at the average maturity of all lines in the trial.

Such a machine would be of great value but it would be very difficult and costly to develop.

14 mean yield groups were separated in a five.year

rlr of o replications using BuncanIs YAM Is Range Test.

This analysis indicated that rather marked differences among the 29 lines were present during the five.

year study.

The highest yielding lines 112 -I, which, incidentally, is current ly undergoing advanced testing, produced significantly more hops than 23 of the

Fuggles the lowest yielder in the test, produced significantly less than all of the other lines except for 144-1 which have been selected for off - station testing,

The amount of annual replanting required has been recorded since

1955.

tate Clusters has required the most replanting during the period, however, 123-1 127 -I and 109 -I, have each required 10% or more replants each season.

Of the lines now undergoi.ng advanced testing in off-station trials in

31 t

p.25.

At Corvallis data were obtained acid and oil content and storage chareetoristies.

1

At Prosser data were obtained on yield and alpha -said oil content.

'field results are now available for 1957 and 195$ from thistrial and will, be summarized, Harvesting at Prosser was accomplished by an experimental mechanical picker,

The following tables present the most important information obtained on the lines in this trial.

Additional agronomic data can be found in the rimental Yield Trial under CRe5-1, Additional chemical data are included in Sections Ai I and C under CRe5-5 report.

Time did not permit statistical analyses of seem of the however differenoes among lines are obvious for several charaoterittics.

1.0s14

Denim

1034

104.1

1074

108 -I

109.1

112-I

123-I

124.1

127.1

lae.1

132.1

135-1

138-I

1394

144.1

17

10

20

14

10

7

214

10

}ban

(%)

LSD (5%)

16

12

5

1450

12140

760

1330

16

600

0

1500

1290

1130

1520

1370

1510

8

330

1540

1320

1630

870

820

960

1770

1370

1280

11

390

1450

810

1430

1920

1100

1320

1680

1170

500

1480

760

940

1170

1330

1280

9

310

1990

890

960

1890

1230

1050

1800

1070

380

1720

850

1190

1910

1160

1180

1740

1120

440

980

790

1470

1350

1280

NMI

9

9/10

9A7

9

I

Underlined yields exceed that of

* Taken from different trial at

Lets Clusiers

Corvallis.

Data supplied by C. N. Nelson

Data supplied by S. T. Likens

Based on remarks made by R. C.

(see continuation, next page)

Cadsby, Anheuser-Busch, Inc. (see continuation next page)

1

I

1274

128-1

132 -I

135 -I

1384

139 -I

14 4

5.88

3,80

7.99

Bunion

1034

1044

1074

108.1

1094

1124

7.71

3.08

3,06

4.14

4.48

5.01

14.94*

5.76

4.22

5.29

9.06*

3.14

1.54

4.74

3.27

3.41

4.23

ate gal

Ma

ita

0.49

0.36

0.59

1.60

.

2.11

.60

0.78

1.47

1.46

3.34

2.89

3.26

1.17

0.83

0.49

1.30

0,80*

0,22

.

0.28

0.20

0.36

0.52

0,88

Orson appoaranoo; fair to good hop; bland but not off.

Strong; fair to good appearanos hoppy;botter than 128 -I &1074

Very good, bright color; oones fresh, clean; one of boot in group.

4.65

3.62

1.90

1.78

1.84

2.43

2.39

2.12

0.75

0.95

0.94

2.16*

0.21

0.85

0.6

0.47

0.61

0.43

0,31

0.31

0.18

0.31

0.49

0.29

0.38

rash, clean, similar

Slightly off - oily in aroma; good appearance

Slightly off (oxidised); good aparance; oily; better than

1pe444.

Fair to good appearancal bland, and strawy aroma; better than

135-1

* Taken from different trial at Corvallis

Data supplied by 3. T. Likens

Based on remarks made by R. O. Oadsby

44

Disealutien 00nOlnsiOn01

At Corvallis, si in the three year summary of yields, for sidearm 1 ture content at harvest time (not sheen in the summary table).

'fields in approximately 200 lbs. per more below the three year average in 1958, due primarily to an unusually warm season.

Surprisingly, Late Clusters did not de

40 W411, yielding only 860 lbs. per acre.

A moderate amount of downy mildew in 1957 is hardly enough to account for the low yield of this variety in 1958.

Seven of the lines outyieldsd Late Clusters in 1958, some of them utuggles" types.

Three of the varieties have outyieldsd Late Clusters over the three year period.

These are 1074, 112-1 and 123 -1.

At Prosser significant differences were indicated for both 1957 and 1958.

Late Clusters was the highest yielding variety each year, and on the basis of the LSD's exceeded the yields of 11 varieties in 1957 and all of the others in the trial in 1958.

These results indicate rather conclusively that observation plots should be established at Prosser from which to select lines for testing in that area. This could be done easily by planting the selections at Prosser which have been screened only for downy mildew reacti ©n at Corvallis*

It is apparent from these trials that considerably more relative vriability is present at Corvallis (CV of 16% in 1958).

There was room for only three replications to be planted at Corvallis in 1955, whereas five were planted at Prosser.

In future years, particularly for "advanced" yield tests less than five replications should not be considered.

Three replications may be sufficient for preliminary testing, however, if space is at a premium.

The alpha-acid and oil data are in line with last year's results.

Alphasiaoid and oil contents at Prosser were noticeably low for the second year.

seesidmring that the

Gorvallls when chemical data are to be

s avid.

will have to be investigated drying temperatures could, at

Only a portion of the lines at Corvallis wars evaluated by ndastryreprementatives this year.

The lines evaluated were those been selected for off- station testing in Oregon and Washington along commertial varieties. Evaluation was nadir by different brew* master than in past years.

It was apparent that opinions of quality differ among individuals.

Mr. Frank Scheeigor liked 1154 and 11014 and didn't like

Late Clusters and 1284. Mr. R. C. Cadsby liked Late Clusters and 128-I and wasn't too highly impressed by 1354 and 1444.

Several of the samP were obviously immature.

This objection will be eliminated next year,

CRe5-2 (oAES 36) THE EVALUATION CT FUNGICIDES aINSIs

.

PMSAtue vezics

S.. 19% Anneal Report, p

Plaid Trials with

7

INIXII cc tieId seals downy mildew control trials using three sprays mmercial hop yards of about 9 acres each.

Treatments consisted of three commsroial preparations of streptomycin (Agistrep,

Agrimyein 100 and Agrimyein 500) applied as sprays containing 1000 ppm streptewein

Two applications were made.

The first application was made

May 3 and 4 when new shoots arising from the pruned crowns were about 8 inches long.

The eeoond, was made May 26 and 27 about one week after the first vine training. Data on number of systemically infected shoots present were taken

May 17 and 19 and Juno 12 and 13, about two weeks after each application.

Plots consisted of 510 hills each and each treatment was replicated 4 times in each

Data on number of hills infected and, incidence s mic infection are given in the following table for the first applications on all three yards.

z

1

Agrimpin 100 2

3

4

1

2

4

3

2

3

7

6

5

S

14

4

2

3

2

3

1

2

1

6

II

4

6

5

5

3

3

9

11

6

8

7

8

5

8

11

13

zi

6

16

It

7 lit

10

10

5

9

20

21

20

5

5

6

6

6

5

6

II

10

9

16

22

18

15

21

20

Cheek

1

2

3

4

43

49

55

47

17

31

22

34

46

73

55

73

28

23

23

31

61

52

78

68

64

75

66

81

150

174

188

188

Data were again sollectod after the 2nd application and awe shown in the next table' incidence of dowry mildew infested hills and sheets after seoond application of streptomyein

Agrietrep

1

Agrimyoin 100 2

3

4

A.grigrein 500

1

It

Check

-4

1

2

3

1

2

3

4

Yard 1

Number of inflated

!fats *We

8

7

10

6

12

16

17

11

5

5

5

4

12

7

9

6 lard

Number

2 of infested WSW)

4

6

8

11

14

13

5 12

Tart

3 number of infected

nail

4

4

5

3

5

7

8

5

1

3

3

4

5

7

14

10

1

2

3

3

2

5

5

4

5

8

8

8

14

17

14

10

8

5

5

5

13

10

12

9

6

7

,

9

13

8

22 49

4

90 apilms reied by

31 61 before data vas o

41 65

Total

M ATT-V:Si

16

17

23

14

2 .1

37

38

28

19

20

22

17

7

10

11

11

36

36

39

27

19

19

18

20

a

*rowers mere yell satisfied with sad mot too coat27. There is still same quest

*eery. Trials will be conducted in 1959 to compa applications one and tee

On the basis of

A.R.S. has ruled that only st of be used on hops until additional data on retention of strop plants has been obtained.

A similar trial was conducted in the YakJme Valley of Washington by Dr. C. B. Skotland of the Prosser Experiment Station.

his data show 70 peroent reduction in spike incidence from a single application.

of 1000 ppm streptomycin spray applied when mew growth from the crowns was about 6 inches.

Later applications resulted in decreased control.

Tests mnducted by Dr. J. N. News of the University of California, Davis resulted in very little control under conditions of their experiments.

Time of application in relation to the developmental stages of systemically infected shoots appears to be an is portent factor determining degree of control.

red b obtained frog each

eats of plants male cted frost the Agristrrp treated

plots and combined to obtain expressed plant sap.

!.salts: of gap expressed from hop shoots treated llected at various intervals after treatment is shown in the table below.

A nazis= concentration ot 116 ifiri was reached 2I hours after treatment, by a gradual decline

72

96

2

3

5

6

14.3

3.7

ita

5.2

5.6

4.7

14.5

3.5

146.0

25.14

23..9

10.8

2341

18 I

X2.8.

16.5

33.0

35.3

21.9

2814

288

3.2

336

)60

014

1.5

1.3

1.2

0.8

1.7

1.9

2.3

1.3

0.9

t tine intervals after the s raptonycin spray and assayed for stpad sap. Data fres those assay are givan in the table balm.

51

3.

3

14

6

8

ID

Ian Inhibitten sew widths

5.4

442 h42

3.2

2.5

ihreptionida osseantra-

,Pon (11114

10Pli

31.11

404

25.6

17.4

8.8

5.4

p

The pattern of absorption and tranelocation of streptomyoin by hops receiving a single spray of 1000 ppm showed a maximum absorption of 46 pia after 24 hours followed by gradual reduction over a period of 15 days.

to greenhouse tests conducted previously (1956 report p. 61) maximum absorption was the same, but field-grown hops retained detectible quantities of streptomyein fora much longer period.

Following a second application of 1000 ppm stroptmaysin 21 days after the first application, the maximum level of streptomycin absorbed was much higher than after a single application on either field or greenhouse grown plants.

This effect oould be due to age of shoots treated, effect of environment on absorption, or afflict of a previous application of streptomycin.

Streptomycin irms found to be readily absorbed by young hop shoots d in measurable quantities for up to 16 days.

of treatments. During the 1957 season dowry lading

Mss unusnallya re axx was not eontrelled is this plot.

The following season

(1950 hill dieamout was very extensive.

salts* data table below shows out among the treatments.

Effect of Cheoal Soil T of Hop Crowns, (Treatments taken in 1958)

Treatment

Chloropicrin

Papas

Wiens

Terraclor

Check

4

0

1

Member of 10 hills doad

I

5

2

3

2

3 4

2 0 2

5 2 2

3

2

2

2

2

En

on ova

1956, data.

Total dead

12

7

11

6

12

Percent deems

304

1,5.4

30.0

40saaienand,Conalusionss

Root die -out was *over* in the spring of 1958

Winter injury was not a factor as the 'inter of 1957.1958 was the mildest in ?seen% years.

Severe root die...out followed a severe attack of downy mildew.

These results support the observations of many growers that replanting is heavy following a severe downy mildew attack.

The summary data table above shows that die.

out was as severe in some treatments as in check plots. whetter Terracior or

Yapam reduesd die-out statistically has not yet been established.

h.

eeeporation

1910.

These tie% nee number of vines per plant fort and preaing, time of hoeing, and the use of various cover crops. Many of these studies were of a preliminary nature and more complete information is needed.

Sines there are interrelationships between field practise and quality or between field practice and disease factors, this lino project necessarily entails the work of specialists in various fields. For that reason much of the work reported in this section has been obtained by cooperative effort of more than one investigator.

In 1958 the following studies were conducted under this line projects

1.

Chemical Stripping and Suckering Trial.

2.

Irrigation.Fertility Experiment.

Effects of Gibberellic Acid on Puggiea.

3.

4.

Date and Severity of Pruning

Trial.

5.

Cultural Studies With Hallertau Hope

VOSS

Duration of experiments

To be discontinued in 1959

sin 190 Annual Deport Ps.

semi of the plots mere he season.

8610 1957 &M al

TUN data obtained in 1957 fish a n of $ residual re of treatment.

They are given in the following table.

The effects of two years of stripping and suckering on the yield of Tuggle.

in the third year (all plots hand stripped and suckered in 1958.

Treatment and rats in

1956 and 1957 wt. (lbs./ DUD

1

Percent difference

Check (hand worked)

38.3

21,2 16

Cheek (not stripped or suckered) 143.0

31.6

27

Endothal at 3# / 100 gal. water 32.1

22.1

31

Endothal at 5# / 100 gal. meter 36.6

22.1

Endothal at 7# / 100 gal, water 36.0

21.5

39

60 an

CV (S)

LSD (5%)

37.2

1,

6.7

23.7

7

5.1

Discussion as d cone ions s

The results obtained in 1958 residual effect of chemical stripping and suckering on yield

plots and the handoworked shriek 41000 there indisated among all yids. Seeendlys the fer the am-stripped and suckered plots were mesh talents* The better the job of stripping and

7* r was the rednetion in yield irk 1958.

It be renenbered at thin point that yields in general in 1958 were mach lower than in the previous two years.

There was only a 27% &moose in yield whore basal growth was allowed to remain in 1957 as compared to a demos's* of 31-60% where basal growth was moved periodically in 1957.

Although those results indicated that complete stripping suckering have pronounced detrimental effects on vigor the following year, it is felt that this operation should still be carried out early in the season to avoid or control 80/92e disease and insect infestations.

This study points out that the translocation of food materials from the basal growth to the roots in the fall should be studied more thoroughly.

See 1957 Annual Report, page

T1 1957 Annual Report, Page 68, plot laysout, treatments, and goner

Tree s a detailed deseription of

B I

II

III

IV

The irrigation scheduling in 195

Inches of water applied to treatment

719 cation on each date in 1958

/Az ugt 7

6 7,131 82 86 ME_

Total

454

1.12

1.16

1.07

Treat. G

II

III

IV

Treat. 1)

1/

In

17

I

Treat. B I

II

III

17

2.26

1.4

2.62

2.45

2.60

2.71

3.84

3.

3.56

2.36

3.12

2.94

2.42

2.83.

3.34

3.79

3.39

3.04

3.52

3.17 24.03

2.70 11.078

2.84 13.06

3.49 16.81

11.42

928

6.35

13.19

Notes Irrigations made on 7/5 and 7/9 mere timed to deliver an amount of water calculated to bring the upper two feet of soil, to field capacity.

Those made on 7/16 and later were timed to deliver an additional 4,46 inches of water beyond that required to bring the upper two feet of soil to field capacity.

kn.

0

CT 10%j I

USD (5%, all)

(5%, moisture 1

230.

Underlinsd yields are significantly lover than check, basis lass.

A. NO

Oat& moisture

EsMed.moisturo

Avg.

a

3%

Alpha-acid pores

3.61

3.41

3.21

3.41

3.68

3.58

2.96

3.!

3.36

3.02

3.41

3.26

A.No irrig.

0.55

D.High moiste 0.42

E.Medmoiaturi

0.44

Avg.

0.ta

CV 7%

Oil content (mils4 3.00 gm.

0.64

0.17

0.51

0.54

0.64

0.48

0.54

0.55

0.60

0.51

0.57

0.56

0.61

0.47

0.51

0.53

hope men of bepe in the sown& low. The rosette reatmemts Ineept all intention trea more than the ohs*. The main responae to f however there was ems response to potassium.

debit* response single irrigation early yielded onsiderahly a nitrogen response

Irrigation treatments affected the alpha.aeid content art the b0141 irrigation treatment lowered alpha -said percentage The appli a onbination of nitrogen and phospborns increased the alphapaoid pe

Oil content was decreased by irrigation treatment and increased by application of nitrogen fertiliwar.

There were a Wombat eef s durimg an irrigation on some of the plots when water from the perforated pipes failed to reach out and sometimes two of the gypsum stakes with the result that in scud cases the moisture tension records were not as reliable as they might have been.

Attempts will be made next year to overcome this difficulty.

f elbow/11U 444

fa bare 1444/4 applied stage. of growth serial:4s study in 1958 was preliminary.

work will be carxied out for another one or.tmo

years.

Oe for rtaJOLAR Chit works

At tlee present time, this has only academia interest.

Since gibbereilic acid has such pronounced effects on other species of plants was decided to try it on hops.

Nature and extent of POOViOna work:

None on hops.

Good accounts of this material and its use on other plants are given in ARS Special Report,

ARS 22-47, 1958 and a technical bulletin,

"fibre", published by Merck and Go. Inc. of New jersey.

Procedure

A trial was laid out with four replications of 1 a 5 hill plots, with n a randomised block design.

Two concentrations of gibbersllic acid in water solution were applied at three different stages ©f growth.

Each ion was applied when the hope wore about five feet high, when they were about 10 feet high, and when they were just coming into flower.

Each plot received a treatment only once.

The spray treatments were applied at low pressure (5-7 psi) in order to minimise drift.

The first treatment was applied on June 11, at which time the hop vines and leaves were wetted until the spray dripped from most of the vegetation.

The later two applications on June 19 and July 2$ were not as thorough, and spraying was done only until most of the vegetation had received some of the spray.

tins sides=

no ow 11.6 fi hig10

503 Pia

100 pin

500 PP=

100 ppm

initial

930 PM

Maas cl (%)

00 (5%)

28.7

240

20.h

18.9

Zh.4

12

TA

7/6

7/16

9.0

94.9

10.7

10.2

TA

7/2

8.8

7/5

6

1.6

h

3

8

13

11

24

10

14

12

N3 ha)

4.22

449

3.60

2.69

MT

0.17

ante had produced burrs

y Data

supplied by S. T. Lilo=

62

63

Fairly wide ranges apparent* however.

There wore to differenoel in length

The most striking aspects of this study were the decrease in cons die delay in flowering time caused by the earliest application of the acid* particularly the 500 ppm rate.

Following treatment on June 11 there was considerable succulent spindly growth apparent a few days later.

By

June 25, hot weather had blasted most of the sidearm tips and the flower buds which had just formed.

Later there was a profuse amount of vegetation, particularly high-up on the plants.

it is believed that much of the flower development on this treatment resulted from a esscend" blooming which accounts for the delay.

It appeared that most of the inflorescences developed into cones causing a much higher count to be present than on the check plots* but these wore greatly reduced in size at maturtV

It is interesting to note that late treatment with gibb acid caused a reduction in alpha -acid content.

As a general rule alpha-acid content is not influenced too greatly by treatments which affect the vigor of the plants, however, such results apply to material whose action is of a different nature.

There were no apparent differences in maturity, supported by the fact that the hops did not differ in moisture content at harvest time.

As a general rule, moisture content of the hops is closely associated with maturity.. within a given season for a particular variety.

Rei4111011.1 for widertak

See 1956 Annual Reports page 104.

See 1956 Annual Report. page

Restates

Harvest weights for 1958 and the 1956 to 1958 tie year flowering date, sidearm length and Alpha-acid content are given in the following

table.

Data obtained in the Tine and Severity of Pruning Trial in 1958

Treatment

Harvest wt.

Harvest wt.

(lbs.hlet)

Date initial burr

.

1958

Sidearm

124

(inches longth

)

M

22 13

33.9

6/24

% alpha.

1958

4.15

A no pruning 24.0

20

B 4ed. 4/11 23.1

33.9

6124 9 4.16

3,52 c Ser. 4/11

22.7

21.7

34.6

6/24 18 8

D Mo4. 4/18 36.6

6/24

23.

11 3.90

25

E Mo4. 4/25 24.9

35.4

6/28 7 3.96

LSD(5%)

23.3

6

NS

34.9

4

NS

..

..

NS

21

4 *

2 *

10

11

3

3.94

--

* Incorrectly given in 1957 report

Differences in sideara length hare been

The significance of this

repox ,axed petition will not be mad* this year year s masa should be expanded to include more than one variety and cover a elder range of dates.

This will be done as soon as possible.

3.'1957 ul

The funds to support this s of studies were not received from the brewing industry in tins to get t relax system established f ©r the

195e season. The trellis WA4 completed during the summer, and some results will be- forthcoming in 1959.

A.

raftxaticn of Strobilss and Flowere, lbw% pigs 100.

(ass 1956 Annual

The Obialcal NViluation

Pew 108).

of Leaves, ( 1956 Annul Report,

Investigations Of Analytical Methods,

Report, page 100.

Strobilas Al

1956

Annual pure ions

Se. 1956 Annual Report, page 109.

See 1956 Annual Report, page 109.

See 1956 Annual Report, page 109.

from the Quality Trial was taken as the individual st9es matured.

All replications were picked at the same time for any particular variety.

Samples from Corvallis.Prosser

were also taken according to maturity, however, replications of a variety may have been picked as much as a week apart.

sperimintal Results*

Alpha --ate determinations were made on 7 experiments involving 2 line projects.

Detailed discussion will be found with those projects.

Results of

NL.acid analysis on advanced experimental varieties appear in the following three year summary table.

higher oCueseist valises than

1354 has been lowest in Alpha

Varieties in the Corvallis T set in 0( eusid produstien.

1957 was the first year for the

(LewieBreen Corvallis) and In 1958 only 10 varieties wore

At the a time of which appear in off - station trials, except Breuer's Geldj.

the

Pressers Varieties fran Prosier, have been very inconsistent during years.' The 1958 were very smell and dry.

The 'Apulia granules were often orange-ibrown, indicating the possibility of over-drying, which may account for the unusually low

06.acid values,

Sacrament's Since 1958 was the first year for Saeranen r,, the hops were babies.

Their appearance was good and °C - acids were normal to high for the respective varieties.

There are two anomalies in the California samples:

1.

135 -I, normally very low, showed 5.69% ,=:,-( -acid,

2.

128-I, normally very high, showed 5.26VC-sicid

Alpha acid analyses have been made on samples from the following

Agronomic Trials (CRe54): 1.

Pruning, 2.

Gibberellic acid, 3.

Irrigation -

Fertility All were the variety Fngg].ea.

Pruning. For the years 1956, 1957 and 1958, no differences in meed content have been indicated for any of the pruning treatments.

Irrigation- Fertilitys Samples were analysed from 4 reps. of law, medium and high moisture plots, each containing Neill, N2Pill, N2P0K1 and

Nei% fertility plots. Differences in (DC -acid content were indicated

(1% level) for the 3 moisture levels, but not for fertility treatments.

-aol4 von

69

L.O.

Fogg.

B.G.

Bal.

Back*

5.e8

4.06

5

5.33

5.88

3.80

8.55

8.11

7.99

5.13

7.60

741

103.1

104.1

107.1

108.1

109.1

112-a

123-1

124.1

127.1

1281

129-I

132 -I

135.1

1381

139 -I

144.1

3.96

3.34

3.08

411

3.47

3.06

4.63

3.97

4.14

4.56

4.28

4.48

5,30 6.30

5.01

4.94*

6.94

6.33

5.76

4.39

3.98

4.22

5.32

5.72

5.29

9.82* 9.70* 9.06*

4.45

3.51

.3.14

2,66 2.38

1.54

5.22

5.47

4.74

4.14

3.78

3.27

4.78

4.90

3.41

* not included in Quality Trial

.45

5.20

8.03

9.43

8.04

647 5.63

5.92

3.41

2.20

5.61

4.24

8.56

7.09

6.93

7.05

8.92

6.24

6.82

6.28

10.30

6,25

3.10

2.46

2.85

2.63

3.54

4.27

5.94

3.52

,plaes from

5.21

6.

4.23

6.75

2.41

2.47

1.60

1.98

2.04

1.47

3.44

3.99

1:46

4.50

5.22

3.34

2.51

4.31

2.89

3.91

5.95

3.26

5.19

6.70

4.65

4.40

2.90

3.62

5.15

3.54

1.90

2496

5.00

5.70

6.05

5.26

3900 1.78

2.38

2.45

4.32

4.90

1.84

2,43

2.69

3.63

2.39

2.58

2.35

2.12

5.69

3.88

4.35

Yield Trial and Breeding Block

Voris

L.C.

Tugs, aa,

Bull.

103.1

104.1

107.1

loa-I

109.1

112.1(2)

123.1

124.1

127.1

132.1

135 -I

138.1

139-1

144.1

128.1(2)

% d

26.4

27.8

31.7

29.5

25,0

26.8

25.7

26.8

26.8

27.8

324

29.6

28.3

22.8

27.8

27.2

29.3

22.4

Orion oil

Color. 8POotr. Gtair. C.0.b.

.73

.99

2.89

2.40

1.50

1.62

1.14

.514

1.03

1.11

.80

1.06

1.17

.30

1.66

.86

.34

.81

2.84

5.9

3.8

8.0

7.7

3.1

3.1

44

4.5

5.0

4.9

5.8

4.2

5.3

3.1

1.5

4.7

3,3

3.4

9.1

7.4

4.7.

10.2

9.5

2.1

1.7

4.4

4.7

6.1

6.3

5.8

4.3

5.6

2.9

1.6

5.7

4.3

2.8

8.9

5.2

3.3

6.4

6.2

1.2

1.4

3.2

3.2

3.7

4.3

14.2

3.3

3.5

1.8

1.0

3.5

2.9

2.1

6.8

5.5

3.7

9.2

8.2

.3

.4

3.9

3.6

4.2

5.9

4,0

3.1

3.4

2.5

.6

2.0

2.7

1.0

3,8

2.0

3.7

4.2

6.6

5.5

2.6

3*5

3.2

2.0

2.2-

2.7

3.2

2,3

3.2

3.8

3.3

3.7

3.2

394

26.0

35.4

39.1

23.4

28.6

24.5

40.4

304

17.6

37.9

38.1

46.4

22.1

148.8

23.3

39.0

23.8

30.6

(1) These values are averages of the individual analysis of the same replications but different samples.

(2) These 2 varieties not from Quality Trial but are from Rep.5 of

Trial,

Yield

72 p.111

Reason for

See 1956 Annual Report, p.

extent of pronto works

See 1956 Annual Report, p. ill

See 1956 Annual Report, p

1.39Ortaente.1 result's

Oil yield meaeurements ware can 4 replicatit levels containing 4 fertility treatments each. Results and discussion will be found under GMe5,4, Fuggles

Irrigation-Fertility experiment.

Oil, yield measurements on those varieties in advance tests have been completed for the third year from the Quality Trial at the East Farm at Corvallis.

The second year's data are complete for those varieties at the

Levisoarow Seedless Yard at Corvallis and for the material at Prowler

Washington.

The first year's data on the material at 6acramento,

Calif. are eomplete.

With fee exceptions, the oil yields for each variety in the Quality

Trial are fairly constant over the three year period.

The same is true for those varieties in the Seedless

Yard.

edless la-la seeded hcmes

However oil yields s sr to be higher

Samples free Prosser were found to be very low in both axd oil this year.

-acid

The cones were small and the lupulin was often dark

by hops and may net be typical. Ths oondition.

of oils 1.

7 Quality Trial by gee

is rrlatt

the oon thin 4 varlet n of each weapons,* measured instrument with which these antiquated and inadaqu consequently the results lack a great deal in precision.

in spite of these handicaps, the chromatograms obtained appear to be fairly specific for each variety.

7

2.89

2.60

2.48

2.11

Buda

103.1

104.1

1071

108.1

109-1

1124

123-1

124-1

127 -I

128.1

129-I

132-1

135-1

138-1

139 -I

144-1

.78

1.17

.88

.04

.70

.83

.49

1.43

1.13

.92

.72

.75

.60

.84

.95

1.34

1.25

.94

2.13

1.30

.80

.52

.75

.48

1.20

.21

.85

.76

.57

.69

.64

.60

.47

1.50

.66

.61

2.08

2.44

1

.79

1.40

1.85

.88

1.59

1.17

1.32

1.03

1.47

.92

1.44

1.42

.31

1.43

.60

1.28

.95

.53

.69

.75

MM

Mellt

MM

GeM

WM

.55

.49

.76

.22

.28

.20

1.22

.36

1.34

.52

1.06

.88

.92

.53

.43

.31

.79

.31

.31

.60

.90

.144

.58

10115

.18

.31

.49

.29

.38

41ml

--

.149

.28

.40

(1)

(2)

(3)

(4)

Each value is the average of data from 4 replioatio

Sines determinatiow

1 replication only.

Each value is the average of data from 3 replications, see appendix.

Single determination -- 1 replication only.

.51

WM

*NW

SPW

OMP

.53

.41

.50

48

Chressie

(VP Crop drY Separated an 10 toot

Lite.

2

3

3.

2

3

28

26

10

7

5

8

7

10

15 a.G.

5

8

8

10

3

6

6

3 li

8

6

7

6

6

7

103-I

135.4

2

3

1

1

1

2

3

4

1

2

3

36

144

55

50

66

58

59

50

53

50

145

30

1 'gyros=

2 Mathrimmosylkatime

3 aphaerearyaphyllese

5

Unknown component present in most varieties

8

5

It

6

5

9

8

8

5

6

6

6

6

6

7

23

18

19

17

15

1

17

6

6

10

3

2 it

Rave additional,

characteristic

peaks neck M.N.K.

High *r.

0

7

7

9

This reperamoved from trial,

r't, Pe

tions

This work is being done in cooperati

Agricultural Chemist

Oresen State Co

Lwerlmentalliesultet writh the De

1957 Crap he past, samples were taken from

Trial est Farm.

One sample of about 5 lbs. green, was taken from each replication of each variety.

After drying at 118°F.

in a cabinet draft drier,

4 sub samples of each were prepared, one of which was analysed immediately for

...acid and oil content.

The remainder were put into 38'F. storage for analysis in December, March and July.

Data from these tests are summarised in the following table.

Detailed data will be found in the appendix.

During 10 months storage at 38°F. there was an average loss of only 7% of the (k'` -acid originally present. This compares with an average less of 27% in 1956-57 Oil losses this year averaged 20% compared with 25% last year.

(h6% and 39%)

Bullion and Brewer's

Told sustained very little

-acid loss this year, (5% and 3% respectively) but suffered heavy oil losses,

128.1, the rich experimental varied* held up very well for the

sit oC 444,41 tor 441

in 0C

t ay.

this Tsar.

ifiAmelts of Storage 2044

0.S.C. Bop lards 190 erip

Sri %, BiV1, Oolorinatria asidesis et is in an reps involved A s even dry hops vitas la

Variolif

1 Lets

Clusters

5.4143) 5

( )

0.48(31 0.47(3)

2 Tunics

3

5.34(4) 5.39(3)

0.75(4) 0.72(3)

6.04(3) 793p3

2.89(3) 2.25 3

4 Bullion a-acid oil

7.60(2) 7.76(2)

2.14(2) 1.32(2)

5.25(4) 5.39(4)

0.85(4) 0.67(4)

7 «801

7.52(3

2.320

1.76(3

7.26(2) 7,20(2)

1.90(2)

146(2)

5 103.1

a-acid oil

3.29(4) 3.19(4)

1.38(14) 1.03(4)

6 1044 a-acid oil

3.47(4) 3.68(4)

1.48(4) 1.19(4)

5.36(3) 5.140(3)

0.39(3) 0.39(3)

3.00(4) 2.88(4)

1.15(4) 0.93(4)

3.26(4) 3.39(4)

141(3) 1.10(4)

7 107 -I a-acid oil

3.97(4) 4.16(4)

1.04(4) 0.91(4)

8 Ima a-acid oil

628(4) 610(4)

0.70(4) 0.67(4)

3.85(4) 3.87(4)

0.86(4) 0.86(4)

3.88(4) 3.70

0.60(4) 0.6

9 109.1

a-avid oil

6.30(4) 5.40(4)

1.13(4) 1.11(4)

4.99(4) 4.72(4)

1,29(4) 1.06(4)

10112 -1 a-acid oil

5.35(3) 4.77(2)

1.00(3) 0.86(2)

4.23(2) 4,88(3)

1.00(3) 040(3)

11 123-I

22 124.1

a-acid oil

6.32(4) 6.57(4)

0.72(4) 0.59(4)

6.47(4) 6.214(4)

0.55(4) 042(4) a-acid

oil

3.98(4) 4.39(4)

0.84(4) 0.75(4)

3.63(4) 3, (4)

0.75(3) 0

13127 -I

14 1321

15135 -I

16138 -I a-acid oil

5.83(3) 5.52(3)

1.25(3) 1.03(3)

5.13(2) 5.42(3)

1.08(2)

1.16(3)

1to o44.il

0.27(2) 03373((43) a-acid oil

2.38(14) 2.40(4)

1.20(14)

0.96(4)

2.13(4)

2.07(14)

0,98(4) 1.00(4) a-acid oil

5.45(4) 5.74(4)

0.69(4) 0,68(4)

5.48(3) 5.56(4)

0.50(3) 0.36(3)

12

13

2

13

14

7

0

148

10

39

46

33

25

17

10

6

10

0

19

26

17

44TAIRA

Art x-6cr

OT

(1)94n

(c)09'0

Pro nog-,

TTO

(tvolen

(1094c

10991 sex

'c

$S`0

(

(n voun

Mom

MIL

Mg

Cream, rio

COMM'

'VI

(I)

Mt=

A a paipulme ;Asp

I I0

C/3

COWL

(T)001Z

(1)WE

9' (T) rt tit

Oa

9'9 ra-P0

*9

LIP

us.

See 1956 Report* p. 115

Nespons for undertaking this work:

See 1956 Annual Report* p.

Natt of preview: smirk

See 1956 Annual Report, P.

sults during the am r a 5 replications of 2Q male lines were collected

r

of 1958 as follows *

When a flowering branch began to shed pollen parchment bag 1411 placed over it and left for 3-4 days* shaken to break antbars etc. loose, and taken to the laboratory where tt was dried under vacuum at 40-50°0.

After all samples had been collected; material from each replication was composited to give a single sample from each replication or a total of 100 samples for the entire trial.

These were held at 0°F. until analysis.

Chemical Analyeii.

FaTerienee with counter current distribution during 1956 and 1957 indicated that a substantial loss of 0/ and 73 acids occurred during analysis, (20.60% loss).

Ion exchan resins stigated as a means of separating acids from residue material; (see "Investigation into Chemical

Methods").

This was found to be satisfactory and analysis for and acids of at least 2 replications of the 20 varieties is well underway method in present use is outlined as follows:

The

with sufficient methane/. to give a Alemeentmatien of *beet 200 rs per *1.

gxtreetien it

5 minutes Ina blender

(Serval sdhsequent oentriteging at about 1500 r*p.lmo

The superna into a polyethylene bottle and maintained at

OPT exempt withdrawn for analysis.

Two aliquotes of 0.5 and

2 inlet are taken for *Wyatt'

One is diluted and read directly at 332 mu on a Begawan Nadel B spectrophotometer and the absorbance recorded.

The ssooud is placed onto a 25 am (9 am appears formate saturated with to be sufficient) x 1 am column of Demexmil x methanol. With the flow adjusted to 0.5 to 0.8 mien per minute, elution, of the c( and/3 acids is carried out by addition of

15 els. methanol followed by (2) 60 mis. 0.3% formic acid in methanol follownd by

(3) 100 mls. 2.5% formic acid in methanol.

5 ml. fractions are collected, made basic with

6N NeOR and diluted to 10 mls methanol.

These are read at 332 mu on the Nadel *B" and a graph of absorbance vs. fraction no. is coostructsd.

acids.

From the graph is determined which tubes carried the

(:-Nc acids present

Absorbancee of these tubes are added and the amount of is calculated. Total absorbanoe collected is compared with the original absorbance to determine approximately the amount of absorbing material which was lost during the analysis.

Experiments have shown that lost absorbing material is neithern; nor cid

Detailed listing of analytical results will be found with

GB05-1.

In general 0C-acid has been found to range from 0.2% to

2.5% while

In most acid ranges from 0.3% to 3.1% for the 20 varieties analysed.

Ca Ms -acid exceeds

-acid, however there is no consistent ratio Some varieties exhibit higher than acid.

foreign aboorbors'i

< .

cVsoid

10 15 20 25

Fraction number

30

Fig.

Typical chart showing the separation of

0-and,-,5 acid from male flower extracts on ion exchange column

is

p.

at of the

1 likely associated with moisture

"fs. nose* is undoubtedly * result of the of I

It is the object of this SU

11114 chemical changes 'which accompany maturity to evaluate some of the physi order that better descrip of vs turation will be available.

ject will continue until 3 seasons, data are accumulated* loosens for undertaking, to extend the information available regarding physical and chemical hangs which accompany the maturation of hop strobiles*

"tyre and extent of previous works

Much data has been obtained regarding the accumulation of ,x-iwacidvand

Atoll during ripening* Some work on the relation of moisture and oil of hop strobiles to maturation is available.

It has been reported that the *starch content of hop cones decreases with ageP

Other crops such as corn and peas depend on moisture content and voluble sugar content as indices of maturation.

Samples will be taken at intervals throughout the a on from at least

afeNl lid ANS of pus of cK- assi

-acid content* hop a criteria for maturity.

will be used for the detersinatiee of

A. third portion sd.11 be dried and used nt and dry matter per sone will

2 as quality estimates and the stilettos.)

'mortise which ars be related to these three evaluate their rlationabip with maturity

XS

?ire samples of Late

Clusters

19 57 and held in frozen storage st 14 to September

These were ussd for preliminary work to establish procedures and provide a basis for planning sample collections during 1958.

It was felt that the plant juices in the hop cones would provide easily obtainable test speoinan and at the same time be very likely to respond to maturity changes. At the same time it was expected that the amt of plant Jules present would be a function of maturity since the total moisture present drops with aging.

Procedure for testing 1957 sample's'

The frozen sample was thawed and weighed.

It was pressed in a

Carver hydraulic press and the first 5 ills of juice to appear was collected.

The pressure was raised to 1000 posoi. and all juice that was freed was collected.

The pressure vas then increased to 6000 posoi and the remainder of the juice was collected.

Nearly all the expressable moisture was obtained at 1000 posit The pressed pad was weighed and the expressable moisture calculated.

Samples of the expressed were evaluated for refractive index,

Pick PfV00 date eater

Turbi

CVO, misty 2

1114

8/19 59.2

1.3390

8/23 55.1

1.3394

8/27

6%5

51.8

10415

1.3398

1.331401

1.31412

1.3406

1.3399

1.3398

1..3413

.623

4.19

649

5.11

xs x8

31

53

9/12 43.8

1.34214

1.31428

1.31438

6.75

( )

(2) estimated that 95% of the juice was collected at 1000 p.s.i.

Using #54 filter.

155

All properties measured mere influenced by maturation.

As expo of refraction, % soluble solids and turbidity responded nearly the maturity since they all measure similar properties.

Although total moistu content for this series is not available it could have been expected to have dropped from around 80% to about 70% during this period.

Expressable > isturs was found to vary over a much older range, suggesting that it might lend itself will to a maturity index.

55 a

Inamosaabla at 6000 Neste

8

It 9/3 013 awataal oat abentaALobwite

29i7

4 9/3 its

The 115. Mlituritr Og$

(Iv lair Ruch ) t riggloo from on the CoU.Rwr Form whioh Fromm*** on opporttmity to adopt toot prosody:roe to trash kepi.

It

a g

r

The foil

9100 n was evolved f

0100 A.M. on Sunny da

These were picked in a few minutes after picking thylene bags and me

These were returned cloth bag for juice

350 g)* rial runs, Samples were ximately 5 days apart, into four packages within the laboratory and processed as quickly as possible*

1. 6004000 grams (Into dryer) Total moisture content and dry matter and acids.

2.

600 germs

(Distill) Oil yield grams (Freese) Cons oount Expressible moisture grams (Grind fresh and press out juice sample) Reducing sugars,

Index of refraction, Turbidity, Soluble solids

and a held the is, A second sample was is the uted with SO ethanol for sa ve analysis laboratory and

1 al ali

At the second collection a samples was ground in the field and a sugar sample taxon. Again a second ample was grand at the laboratory and prepared for sugar analysis.

Results of these tests are shown in tablet. From the results it was concluded that samples could be transported to the laboratory

Table 2.

The effect of time after picking on concentration of reducing sugars in hop cone juices. # Ong glucose/m1 hop oons Ni4O av of Licata determinations

Date

W11111110.

7/17

Sample No.

1

Pressed i 600 p.a.i. in field

22.9

Ground in field

IMMO

Ground in laboratory

22,6

2 29.2

4140 26.8

7/22 1

2

16.6

15.5

15.9

16.1

* Samples taken in the field were about 20 minutes after picking, ground in lab. were about 2 hours after picking. Variety Fuggles.

Method of Somogyii, J.B.C. vol. 160, p. 61, 1945.

Samples for processing without fear of altered reducing sugar values.

From juice samples taken in this manner, aliquotes were withdrawn for soluble solids index of refraction and turbidity measurements.

Results of the preliminary trial are given in Table 3

80.7

54.7

53.3

5144

PA

50.3

(Spootro.) % 6.2

6.0

5.2

5.3

5.7

5.8

6.0

i'actid (8Peotro.)

Oil Yield (Omen hops)

(a1s/100 g, dor.b.)

0.78

0148

3.7

3.8

2.9

2.8

042

0.99

0.37

007

2.9

2.8

1.32

1.23

2.1

Reducing Sugar

(ag glimpse/ at juice )

23

27

16

16

114

16

19

Soluble solids

5.7

6.5

6.5

6.3

6.14

6.14

6.2

6.5

6.8

(iCIett

units)

155 5 268 394 200 720 2650

18

11411

406

Index of refracti ©n

1.31418

1.31

1

1.31410

1.31418

1.34

1.3406

1.3 17 1.3423

1.3402

Cone weight

( mg d.m. / cone)

231 268 283

0

710.

at the beginning s were just lowing Tables out of burrs Results of these tests are and Cr aphis.

Save we actumulated in a different r for Yuggles than for

Bullion and Late Clusters.

In Fuggles the 0C-said was laid down at about the same rate as dry matter from the outset and held up until 4 weeks miLt the normil harvest date.

In Bullion, cc -acid accumeated faster than dry matter until about midsseason4 then appeared to fall off steadily.

Late Clusters Showed a steady Louses* its %

-acid until a few days after the normal harvest date, and began to decline after that. When calculated to milligrams

-acid per cone betas approximately the same picture is presented.

2.

scids appeared to follow dry matter for all three varieties for the entire season.

Ito loss of

-acid accompanied the build-up of

-acid in contrast to earlier beliefs.

3.

Dry matter content increased steadily throughout the season.

Since the overage dry matter per cone also that % dry matter indicates increased over the entire season, it suggests

=actual accumulation of tissue rather than a simple drying out process.

varied

4. While dry matter content/from approximately 15% to 30% over the season, expressable moiaturs ranged from

65%

357

.

Teets on physical and shemical propert:

=option of reducing sugars', indicated nature.

tion trends i The and et.

f refraction, us .associated with soluble' rose measureibly for all varieties.

2.

Sol solids followed almost identical curves as the index of ref ty: a property often associated with collodial protein tractile increased over the season steadily with Late Clusters and Fuggles, but rose sharply about a week before normal harvest in the cease of Bullions

6 For the three varieties tested for reducing sugar content of the juice from cones, three patterns were observed.

Only Late Clusters exhibited the expected drop with leveling off around harvest time.

3"7"-orws

MTieir

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9t r

g'it r6t

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Study

1/1

a.

Fogg. 1.3430 1

Doll. 1.3414 2.3407

L.C.

1.3439

1.3439

1.3450

1.3458

14421 1.3409 1.3424 1.3426

1.3397 1.3403

1.3405 1.3412

1.3459

sp.

1.)456 1

1.3421

1.3418

1.3425

1

NNW

Terbitty (Juice) Klatt wilts filter 54

rm.

294 350 1068

Bell.

381 650 2900

887

968

3100

732

1412 1212

9000 7650

936 1597

2070 1870 2550

1750 2150 2050 2650

Solids (ass) % Solub

Pun* 7.4

Bull

L.C.

5.5

7.2

5.7

7.5

6.3

5.6

3.8

3.0

2.7

8.2

7.1

5.6

8.8

7.3

5.9

Reducing Sugars (Jell's) ag

.glucose / ml juicae

Pegg.

22.8

Bull.

23.9

L.C.

8.4

6.1

8.1

6.2

9.0

6.7

40111.11.

6.5

24.6

23.5

20.6

21.8

21.6

13.5

10.1

943 10.4

9.18

20.6

23.4

15.2

13.9

20.9

17.5

18.6

--

11.7

8.1

9.3

8.89

40.8/1, SAP

8/20

Expressable moisturs dr

%Mem rulteas

144

,V0,410,10%

8/10 9/9

/29 el2o

"'

9

-

Pukes'

40,

PA9 9

a

8/20 8/3©

So lublo Aids

- Bullion

Tug los

A -Lie.

Salim

0* ragtles

L.C.

8/30 9/9 fr19

shomical pr000dures.

relative Imo hops.

1.

of f GC.. and mhep acids.

Several reps and/s

Bach male flower ran from a sample of pure 47( accompanied by a covery de covering of 4 mg of P( ...avid ranged from

90%.

These were used as correction values for the associated determine..

or and aids in melts flowers.

A single recovery of pure gave only recovery.

As * consequence vomiter current distribution of male flower extracts was abandoned for the determination of theire-?( and ooutenta.

2.

Ion. exchange resins as a method of isolating and determining the ard,,

-.acid contents of male flowers.

Preliminary experiments indicated that Dowex40.formate vouli hold

-acids selectively from methanol solutions.

An early column of

200 mesh Dow x -1- formats 1.2 cm x 50 em was prepared and 1 ml of a methanol extract of Bullion hops (equivalent to about 5 mg. of o(sacid)eas added.

Thit was eluted with 0.2 N formic acid in methanol at 0.5 mis per minute and 5 ml fractions were collected.

These were made basic with NaOH and diluted to

10 mle with methanol.

Absorbency readings were made at 332 mu on a model

B spectrophotometer. Three fractions were isolated in this :tanner and their absorption spectra indicated that 2 were

-hop acids.

See figs. 1 ec

lute

P'Separationor <X7 -acids from methanol at of hops on ion exchange column of Dowexlwformete on solution; 0.2 N formic said in methanol.

Subsequent trials indicated that much better separation could be effected d by employing a stepwise elution technique.

Also it was found that the elution rate could be controlled much easier by using a coarse mesh resin.

A solution of pure ...acid (from the ortho.pbenylene-diamine salt) and pure,

(crystalline

9243°C) was prepared and separated as follows;

1 ml sample containing 2.7 and 2.5 mg of(i-.

...acids was added to a

1 am x 25 cm oolumn of Dowsx.104Ormola (30 neutral methanol and shielded from light with black paper.

50 ale of freshly prepared 0.1 N formic acid added and 5 ml fractions collected at about 8 minute intervals

(5/8 mls / min) Tintse were made basic and diluted to 10 mls with methanol.

Absorbancy readings were taken at 332 mu. Absorbanay at 332 mu was also measured for the original solution and recovery calculated

Semple

A3321 ml g08

Original solution

Fractions collected; y-aad al 87,6

.aoi4A17.7

extraneous 0

205,3

102 a QC.

a

(3 ..aoido

w000lution of the

2) with a dissolving in methanol.

Seigle

Direct N OR extract our.

Mb OR extract partitioned in pet. other

Thews results suggested that better absorption using the petroleum ether partitioning of a methanol that the amount of extraneous light absorbing material could be reduced.

In conjunction with the previous recovery of oC ...acid and (-.3 -acid absorbency, it was concluded that the loss of absorbency from the methanol extracts was not because of a loss of oC - or (-3 -.acids.

Treatment of resins from columns showing a low A 332 recovery with strong H Cl failed to remove any A 332 absorbing material indicating that this residue is extremely tightly bound to the resin or trapped physically within its molecular structure.

Prom these experiments it was decided to make ion - exchange separations of OH extracts of male flowers directly, rather than to resort to the more complicated method of partitioning into petroleum ether.

In a later experiment pure

6'\.

- and 1::' -acids were added to a male flower extract which had shown only of the added (_,{

-

55%-59% recovery ©f A 332, 93%

-acid absorbance was recovered.

A using the Aldo

A for hop extracts.

The preliminary results lists l b regression

Ver3r oua aging.

Methods A large sample of male flowers is ha

?MOH to give from 100 mg to 200 mg male flowers per al of141004 One portion of this extract is analysed for.aeid by the ion exchange method.

A second portion, (equivalent to about 2 grim of male flowers) is partitioned into petroleum other.

An aliquot. of this is diluted with all Me0H and read at 275 alp, 325 sup. and 355 mi on a

Beckman D

U.

Table /-f Analysis of Male Hop Flowers by Direct Spectre photometric method vs. the Ion Bxchangs Method

Direct Sport,.

Ian

Kgacict

1957 M

1958 M coOposite

5214 (Rep.')

1058 Ma 3171,2(Rapa)

1958 M,F, 320.1,2(1414.1)

0.36

0.16

1.31

1,32

0.61

0,40

2.

1.22

0.42

0.22

144

1.40

0.67

0.47

3.08

1.09

The fractional distillation of acide.

Bates (Chem. and Ind., No. 41, p. 1319, Oct. 11, 1958) has described a simple apparatus for the *fractional vacuum sublimation / distillation* of high boiling compounds'

Such an apparatus has been constructed and pre liminary results have been obtained for the separation ofe>,(-- andp. hop acids.

Briefly, the apparatus consists of an "oven" constructed from a plea'

50 416 ling end 2 es

22 niebroms eft e so that at o end t

0 to 0.5 tureefea. This was 'rapped with fiberglass taps re of asbestos paper then red with a larger piece of glass heating element was then connected to a variable transfomrr,

At 15 volts the temperature gradient over the length of the tube was free

150PC to room temperature.

The sample

045 mg 1140C-war/Si...acid) is placard in a tiny put into the sealed end of a piece of glass tubing $0, am long and

This is evacuated to the lowest possible pressure, sealed and the furnace.

Separation requires 1..3 hours.

The tubing is then cut into section', the sample eluted and measured photometrically.

At 1544) and 350p, presaure//,-;.acid was distilled from 0.3 al of a male flower extract containing the equivalent of 100 mg M.F. /m/ or about 0.3 mg ...acid.

The K- ...acid, however, remained with the residue.

At 120PG and 101.4 preswore,<er'acid was distilled from a similar sample but still remained with the residue It is anticim pated the or .acid will be,distilled when pressures less than one micron are used

It is also anticipa

CI lie collecting the essential oils from extremely small samples.

Collaborative rk with the U.S.B.F. on analytical methods.

Spectra hotometric analyses and iou- exchange analyses were done on duplicate samples of three varieties.

Results of the collaborative efforts are customarily reported elsewhere.

mat able moistere and stage of close relationships mere found, that each measurements could be used' such in the manner of soil moisture tests by resistance methods.

To this end tines preliminary experiments have been run to establish methods and apparatus.

They are presented below in their on form4

Electrisal Resistance Weasurements in Hop Tissue evaluation of K 01 electrodes.

Nei* s

To determine if saturated I Cl electrodes with a strinewiek contact mould make satisfactory-contact with plant tissue so that resistances measurements could be made reliably.

Apparatus nu glass tubing was drawn to about a 1 mm bore and out to about 6 can length.

A 1 cm string was inserted in the drawn end and the tube filled with saturated I Cl.

A #00 stopper was cut about 0.5 cm deep with an 8 mm cork borer and cut off at about 1 cm. A small hole was drilled through the center and a 5 cm length of fine Pt wire was inserted through the hole.

The stopper was than pushed down over the tube so that the tube fit into the slice made by the cork borer.

A standard volt-ohmpappere test meter with a 120, used to make readings. The probes from the instrument were inserted into the stoppers of the I Cl electrodes.

e1 resistance values, p=

A ) indica

Calculation of resistivity that the cross sectional area of the different stems accounted for diffe rent resistances encountered,

ObJeets Thin me probes which could be inserted into the stems of hop sheets would make it:easier for readings to be made.

These might further make more reproduceable contact than K Cl electrodes.

Apparatus, He 25 hypodermic needles were silver soldered to the leadends and insulated with tygon tubing.

silver solder

Hypodermic needle gon tubing

Latex tubing

Lead

Me de A piece of iksulating wallboard was ruled from 0.25 cm.

Hop shoots were laid on this board and one electrode inserted through the stem into the board at 0 cm.

The other electrode then inserted at intervals to 25 cm.

Resets:

Resistances were just about double those from the K 01 electrode.

Resistance was not linear with distance, curving upward.

The order of resistances between shoots remained the sane at all distances.

If these electrodes were used in subsequent studies a standard distance would be required.

to K Cl.

From the standpoint of manipulation these electrodes are far superior

1

3.

2

1

2 t NO.

1

2

1

2

1

2 tl

137

1614

110

125

140

125

115

132

100

174

200

180

165

210

153

189

290

310

215

230

270

240

220

260

220

310

290

320

290

470

370

1420

1400

370

430

370

333

163

160

160

160

134

135

125

155

160 tas of variation in resistance men ute between nodes, plants and s

In order to determine changes in the realm of ha maturity progresses it will be necessary to select a site of ve the most uniform results.

This expo what section of the main stem measurements can be made variation leas than exists between shoots or between plan

8 to 10 nodes have developed.

over

To Cate about

Apparatus: Stainless steel described in experiment u, were taped to a piece of wood in such a fashion that their tips were exactly 3 cm apart

Readings were recorded for the first 3 cm of each interned* beginning between nodes 1 and 2, about 1 1/2 inches out of the ground.

These were repeated on three shoots from each of three plants.

The statistical treatment is them nodes are the main effects, plants are within nodes and shoots are

among the o the various but that a* much plants.

00131IT'S plants as of the m ltiple range test suggests that imasurements made at any of the first Live nodes and still obtain less variation than would be encountered among shoots or plants.

Nodes (am iwats (within)

(withi)

$ 033)623

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116.0

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122.3

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Li,

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1

1PPZ ID II

0

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1.1

1.2

1:4

2.1

2.2

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243

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85

154

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1063 2036

1082 2049

3059

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2028

1032

20141

1052 2042

1077 2002

1023 2039

3016

3009

3038

3064

3057

1043 2035

1044 2078

1020 2059

101,6 2068

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1087

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30114

30014

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30143

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1074 2071

1011 2026

1013 2032

3024

3001

3073

3059

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10149

2088

1026 2062

1027 20014

3061

3097

3086

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1094 2076

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3032

3029

3096

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1002

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3017

3053

30148

3070

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1015

2091

1065 2098

1022 20145

2099

3005

30314

3075

3090

4056 500a, wok

4022 5017 6015

4048 SaIii 6051

1 ol2 5019 6034

4035

14033

14051

5009 6037

5025 6009

5031 6018

4052 5059 6040

4003 5003 6032

4014 5028 6013

4042 5055 603.1

4041 5035 6002

14029

5007 6023

4060 5037 6052

4018 5051 6050

4021 5020 6005

4037 5047 6019

14030 5008 6020

4011 5050 6016

14046 5014 6010

4058 5022 6038

4057 5023 6026

4049 5027 6027

401.6

5033 6006

40314 50314 60414

4039 5024 6041

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1.6

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31 37 21 23 33

4

113

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4 15 21

4 8 13

1 8 6

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13 34 A

36 8 20

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17 21

24 23

121

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41 34 19

29 23 32

36 45

29 20

28 46

39 21

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20 25

27

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26 34 33 31 25 24 21

25 14 11 17

9 19

9 20 14

32

3 10 29 16 18 25 28

29 IQ 47 19

11 11 10 29 33 27 23 34 55 65 17

9 10

8 13 17 34

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37

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28

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29 42 al 38

16 33

42 31

45 23

25 35

34 24 27

30 23 25

22 13 23 26 22 30

24 33 27 40 26 33

31 33

49 30 34

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28 32 8

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44 15 46

19 24 24

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20 35 33 16

141

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34 28 21

9 11

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10 28 22

21 40

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21 35

10 15 11

18 22 25 25

18 44 33 19 52

4 11 17

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29 28

28 23

45 23

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23 80

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11 24 15

20

214

18

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17 27 11

114 140

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11 18 31

16 10 18

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16 33 17

980 61.1000

432 466 489

51 220 272 299 558 523 582 738 702 860

312 31410

26140 14989

12195 21232 17962 10116

34250 114628

22682 31008 41320

1429114

8610 11023

120

33

29

36

26

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314

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24 16 25

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22 19 13 30 7

28 34 18 21 8

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17 17 16 26 13

16 31 20

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18 13

19 11

23 11 37 16 23

38 23 21 38 14

21 41 24 16 16

39 1.9

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19 11 31 27 16

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21 15 12

218

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534 576 500 673 356 413

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63

65

60

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83 67 49

71 57 143

68 91 41

58 61 27

54 74 68

62 63 47

50 65 60

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66 64 43

63 50 51

141

72

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63 56 30

45 65 30

66 70 41

67

55

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1148 1.6©3 1122 385 1405

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15

11 35

11 9

27 23

17 13

14 7

18

114

25 27

19

12

13

21

31 18

114

22

9

18

13

12

19 15

12 10

114

22

1,5

13

15

314

114 214.

24 23

114

15

2

91/4 400 716

38578 21596

7168

130814

3.508

10896 19475

6382

7929 87960 532314 65113

106047 7653

7397

26 23 40 64 31 48

28 27 26

142

24 38

35 le 26

146

37

149

28 47

314

23 3.6

23

37

32

37

23 28

n

26 yr

44-4

61

39 42

142

34 32 59

24 16 29

314

28 66

21 4 6 30

25 26

214

24 15 50

34 19 56

43

144

35 38 29

29 19 29 60 140 50

34 33 33 46 39 32

45

45 32

25 34

1114

39 22 58

33 9 36

13 39 33

21 22 36

25 28 24

3o 25

143

38 38

35

34

33 22 37

35 40

68 36 48

69 33 38

22 2 5 32

20 28 42 62 32 59

19 2 3 49

36 23.

32

714

10

31 39

29 33

45 10 51 44 30 44

53 25 34 67 35 30

1170 852 1089 a 717 615 877

993 1164 eve

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22361 32749 59228 50265 44263 27398

17283 29986 58376

28

143

37

69 38

63

214

36 74 36

34 56 37

35 2 41

A

148

2

7 3 5,7

39 56

2 9

146

30

27

39 34

30 57

22

29

39 29 21

59 56

314

18

43 22

36 33 50

33 44 27

146

37

47

45

38

29

55

62

61 31.

59 34

7

40

35

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23

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19

27

2 5

3.6

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2

34 34

24 17

4 21

15 25

25 35

34 24

28 28

27 24

26 17

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214

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973. 625 611

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35 37

43 42

38 43

39 47

29 36

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39 2

36 29

35 35

28 28

33

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2

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32

32

3145

31

27

40

51

30

40

35

32

26

43

141

140

41

31

931 909 927

35769 355145

314631

32

21

17

13 32

18 9

24 19 27

29 1 3

3 2

17

28

23 22

16 16

24 26 17

21 20 24

20

16 13

19 24 25

19 33

22

16 11 14

20 18

34

214

20 21

29 23 28

22

18

15 26

21 18

15

11 21

26 25

26

19 20

214

37 52

48 26

1s9

54 63 41 26 23

3 25

3 26 29 32 48

35 45 53

14 14

59

6

22 24 38

26 27 39 a

42 5 57 95 26 35 29

59 36 47

25 39 52

50 27 72

83 50 64

85 46 70

59 50 56

35 22 28

28 16 21

49 18 32

35

144

39

79 51 62

32 58 30

37 38 63

68 49 65

78 65

146

30 30 27

27 18 41

15

314

12

37

148

63 28 32 17 35 39 62

58 41 90

48 43 52

55

146

37

60 65 51

31 40 75

141 73 59

57 43 67

64 62 52

72 63 51

54 72 71

35 32 15

37 33 28

33 23 33

36 24 27

32 23 44

1058 1107 1340

15148 1367 1597 810 668 697

47

43 39 s1 541 00 537

1114 945 20145

512

12739 12513

9594

149192

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761428 1008142 1014861

77463

18732

2 130 21589

551420 146237

37393

65 32

47 32

27 5 ils

57

53

57

38 20

4 1 4 8 5 0

72 51 31

32 53 41

36 30 54

35

35 48

40 33 53

49 36 51

52

28

144

148

414

44 3

35 46

32 20

33 59

51 37

27

0 the at Iamb Peel hilikalines, 1958.

(14)424

2 3

669

92

462

462

428

425

427

76

24

2433

652

7 1A8

95 61 275

79

86

71 294

64 290

8 89 318

7 46 264

287 3147 314

37

122 108 104

106 93 120

84 118 217

118

144

1:

128

319

3 3,

392

2965

39.5

611 207

29.4

931 909 ,27 2767

36.9

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1 109

2 129

155

134

5 la

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0 124

(16)

4

92

1 28 21

1 66 63

2 77 65

3 67 72

4 87

5 92

66

91

Si 514 470

(19}417

2

3

79

28

97

20

80 123

110 113

118 97

13.8

115

135

332

3

102 318

28

82

77

211

70 212

70 209

90 243

95 278

Total

1 2

(18)523

3

Total

3.

(4) 3

525 491 556 1572

71 50 88 209

108 112 139 359

96 109 141 346

78 126 149 353

70 210 109 289

110 109 158 377

412 271 367

77 52 83

157

200 182

207 205 232

223 207 204

220 202 235

252 230 294

537 1548

20.6

1058 1107

13140 3505

146.7

15148

1367 1597 14512

60.1

Total

1050

212

539

644

634

657

776

Total

292

74

312

352

370

467

3.

(20)524

2 4 Total

228 166 219 613

66 26 42 134

162 155 138 455

139 163 133 435

189 158 178 525

169 326 152

1149

191 249 183 523

Grant

3.t._1

11855

2521

6454

6519

6561

6414

7006

Avg.

7.9

1.7

4.3

14.3

4.4

4.3

4.7

668 697 2175

29.0

2144 945 1045 3134

41.8

47360

31.6

outer surface of sepals inner surface of sepals anthers

103.1

26.5

214

106.1

25.6

9

107.1

27.3

1064 22.1

10 109-I 25.1

11 1124 26.0

12 117 -I 23.8

13 118.1

27.2

14 121.1

26.0

15 122.1

24.2

16 123.1

22.9

17 124-1 24.0

18 125-1 29.5

19 126.1

27.5

20 127.1

19.9

21 1215.1

26.6

22 130.1

25.0

23.131`1

23.4

24 132.1

24.3

25 135 -I

26.1

26 138.1

26.5

27 139-I 27.9

28 141.1

24,8

29 144.1

23.9

0

23.0

21.3

24.6

20.t

24,3

27.4

26.3

24.6

24,1

23.6

24.0

23.5

27.5

26.7

24.0

26.0

24.7

23.4

24.4

24.0

26.3

26.7

23.6

23.2

22.6

27.0

26.0

23.5

21.6

20.2

23.7

24.9

24.1

23.6

24.4

24.7

27.2

27.8

22.3

26.6

26.0

24.2

26.7

25.6

25.7

26.9

23.7

26.6

741.4

722.4

739.3

2203.1

19137.84 18094.04 18957.77

165339.69

IS..

11.6

78.1,

90.5

78.9

66.6

76.9

77.9

66.4

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61.6

73.8

76.7

74,2

71.4

71.3

72.2

84.2

82.0

66.2

79.2

75.7

71.0

75.4

75.7

78.5

81.5

72.1

73.7

23.8

24.1

28.1

27.3

224

26,4

25.2

23,7

25.1

25.2

26,2

27.2

24.0

24.6

30,1

26.3

22.2

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Wil kaj

ill

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414.6

16 323 1

17 12k I

18 325 1

19 126 1

20 127 1

21 128 1

22 130 /

23 131 1

214 132 I

25 135 I

26 138 I

27 139 I

28 1141 I

29 1114 1

614.8

I 454

110.0

36.7.

1.8.5

86.0

51.0

14644

36.5

32.8

21.5

4.6

314

540 21.0

56.0

148.2

64.9

1.8.4

314.7

60.5

39.0

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21.7

142.2

23.0

54.7

114.14

20.6

68.5

124

0.6

13 .7

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47.5

147.6

1491

0

314.5

26.2

214 224

18.6

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took

176.0

tow

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226.3

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116.8

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280.14

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114.7

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140.6

282.5

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119.4

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240.3

53.5

66.14

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116.3

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167

Wel

90.0

1630

1770

794

279.8

1900

1340

1390

140.0

115.0

173.9

1180

542

(66.3) 2314 1580

4602 1454 21414.8

1660

344

32.8

208.3

11420

43.9

(40.9) 219.1.

55.6

54.9

11490

2463

1670

363

27.6

14.6

11.8.2

1010

5998

62.0

31.0

1106

56.0

288.5

56.5

58.2

58.9

272 .7

1960

1850

610

31 1281.4 1225.4 12263 11117.6

1190.6

606.3

11420

Ea

2

643112 .82

61175.36445

Harvest Weights (11:124/1210t) in the Experiverntil

TWA Trial, 19544956 inolusive

Entry

Hep.I

1

9511

1956

1--rigg1es 3%0 134g

2 L c 91.7

34.4

168

53.0

3 101.1

36.5

142.8

48.6

67.6

36

5

4 102 I 49.2

50.4

56.8

5

103 I 105.2

460

66,5

6 204 I 48.4

24.4

37.7

7 106 I 54.4

368 47.9

6346.1

58.7

66 .3

1414.3

88.1

8 107 I 95.2

34.3

63.7

9

108 1 10908 8.8

42.9

10 1091 91.4

16.0

38,8

11

12

112 I 116.6

37.2

74.6

117 I 46.2

31.2

45.1

13 128 I 99.0

72.0

48.7

69.3

63.5

88.4

50.8

81.9

14 121 I 78.4

36.0

63.4

15 122 I 88.4

43.2

56.8

16 123 I 83.5

29.2

80.5

62.7

66.14

57.0

55.9

143.6

17 124 I 76.2

51.6

71.5

18 125 I 74,0 141.6

58.0

19 126 I 83.2

32.0

6348

20 127 I 101.2

17.6

32.6

21 128 I 83.5

54.8

5141

22 130 I 89.2

34.4

62.9

23 131 I 46.7

26.8

43.1

24 132 I 81.3

24.0

40.9

25 135 I 73.6

36.8

53.3

54.9

86.8

64

4

147.1

53.2

534

26 138 I 75.0

12.4

43.6

27 139 I 89.4

50.8

44.8

28

1.141 I

29

1144

73.2

91.0

59.6

70,6

60 47.7

48.8

73.0

92.3

146.9

HI

512

1958 Total

33.8

177.3

49.8

276.9

24.5

186.9

35.3

2514.8

65.6

3400

554

2314.9

51.4

232.8

WO 325.7

224 252.9

37.0

2146.7

64.6

381814

31.1

2104.4

61448 366.14

45.9

47.9

286.14

3024

36.7

286.9

86.0

3141.2

46.4

263.6

32.8

2144.9

24.6

230.9

5409

56.0

306.6

64.9

228.6

3467

39.0

3314

234.1

2564

1.7

201.7

142.2

3004

514.7

35044

20.6

210.2

lg

.

100.6

36.8

140.5

28.14

5

39435.2

514,3

91.8

56.4

748

67.9

33.6

62 38 ea

59.6

99.2

14.9

5944 1444

56.8

52,3 1,2,0

2524

119.8 WA 55,3.

86.3

38.1

3143,

11592

129.1

104414

128.2

140.0

103.0

143.6

60,2

42.0

70.9 1064.

72.1

56.5

82.0

33.6

65.7

83.5

300

24.0

109.0

53.2

65.6

37.6

70,6

80.9

627

3%3

71.7

400

6144

81.2

60.7

63.7

53.6

92.8

4..0

34.6

37.8

86.2

56.14

3906 6262

73.6

143

674 28.0

68.3

61.9

69.0

25.6

16.14

Ma 6245

148 .9

7803

58.8

29.0

594 34.8

454

140.6

2

370

89

88.0

148.o

57.0

73.9

86.8

73.0

66.4

640 66.7

544

76.8

103.h

814.0

146.8

147.2

61.1

82

9.2

364

55.7

294

53.7

21.5

31.3

21..0

he.2

USA

6%5

59

68.5

WWI

124

2282.2

982.8 1543.5 1756.7 1288.11

7053.6 2427.3 1122.8 16674 00%6 1225ek 11$24

192887.94

86552.47

40566 56

64342.82

113620.43

218607.23

2213082.82

100708.

5052664

61175.36

2243606 th55517.0

Co'

Ili 1 :Inn ari 11%

vatitOPI cteei

ir.et

Mit

AOAIRV1 lwAUSWIAMA samgenteaqictnilvvit nill olsttas egPae=akiiiAReM22APA

'11'2"IuetiTilm'-°°1-1:141111-41411 ma,h(smogm... iiii4igftaaAsovz innn4n0001

SAACA''a4in aPA"""1410,53gAPt2°'

A llavvvrinctn.d.AnlarinvponwT0111

map-viliwgzagmal

.n

alaREMEEMEnUAA

nnlInsno.numil:nsfillinitletura

geM12gg436(22'32AR=7**Ae4A g v7In

114.1s1.,21;;IIgm111,dri .Amps

1W52Vont$1=Wilga34120AVAAW.2

cvin1150.tinninivinsiilinnnonno.

mmgig°:mcalAw4ggvt(gr-a142-4 vierv11:111 p-wp-.v3Au.

nwillvilcmlmcuctct

WarsIggIWPriiU

HNHHHHNHMHHHHHHHHHHWHHHHHHH

ROW4n5R4M°455

sot-goos$0 PI= "VliggPtrctir= M

N

129.

sic sx2

819.9 3,540.4 6,558.4 6,9944 4885.7 31.798.6

3,494933.63

1,521 340.52

490,00946

892,079.09

1083,624.30

35 870 051.28

130

28

2

56

86 Total

Harvest weights (

Lim

Rope.

Total (1as)

) 109

11

14

28

19584

202351036

369.655

15,148.505

,169.196

726.8227

ligfr

9244138

1141.7291s

Ramat weights (lba./Plot)

95144958

Linea 28 50,138,837 1,790.6728

Blocks (yrasuld reps.)

19 202,473.638 10,656.5073

Error

532 U2,493.978

2114548

Total

579 365406143

131

1 'L.C.

2 103.1

3

1044

4 107.1

5 108.1

6 109.1

7 122.1

8 223-1

9 124.1

10 127..I

11 132.1

12 135..1

13 138..I

14 139-1

15 144.1

16 B.G.

sx2

I II

In

24.1

24.0

26.2

224

25.4

27.4

22,5 21.9

21.9

26.1

25.5

254

28.8

23.2

27.3

24,1 23.6

22.7

22,7

260

25.9

23.9

25.0

24.9

23.6

22.9

24.1

22.7

25.8

29.7

26.1

28.4

27.5

25.6

24.1

25.6

25.8

25.4

26.3

28.4

29.8

26.9

24.9

29.7

25.3

31.2

25.3

30.3

408.6

409.1

4060 122567

70.2

79.0

66.3

76.7

76.5

68.6

79.2

73.8

70.6

80.8

79.4

75.3

77.5

85.1

75.5

91,2

1o506.44

10484.32

10563.37

9 4478.31

As

23.4

260

2261

25.6

25.5

22.9

26,4

24.6

21.5

26.9

26.5

25,1

25.8

28.4

25.2

30.4

Source of Variation

Lines

Reps.

Error

Total

2

30

47

194.01

.04

61.32

255.37

M

12.93

**

.02

2.04

ilorrest, OorililliaPriste

1

1$8.0

524

49.7

5 108 x 7144

6 lop I

45.4

7 112 X 149.8

8 1231

45.2

IP

124 I

10 127 I

24.5

39.9

11 132 X

12 135 I

52.9

314.4

13 138 I

14 139 I is 314 I

18.1

37.7

26.8

16 Bros Gold 1.9.6

SI

512

29.7

34.6

45.0

21.6

47.0

19.0

28.4

37.1

60.7

28.7

30,5

55.8

621.5

28392.03

590.2

214125.24

37.7

.6

.2

32.0

36.2

40.2

30.2

27.4

58.8

18.6

53.14

663.4

30312.64

1883.1

2376714.25

75.8

141..8

1614

1,140.3

11450

104.3

170.8

109.4

87.0

105.8

127.7

109.6

67.1

1143.5

614.4

128.8

1830

1590

1640

1180

19140

12140

990

1200

1450

12140

760

1630

730

1460

1330

133

a in the Co

1956

Rep.I

1957 1958 Total 1956 1957 1958

T

Re III

1957 1958 Total

58.5 55.9

114.0

128.4

48.4 83.9

2144 156.14

29.0 55.8 37.?

122.5

1 L.C.

2 103-I 31.2 60.2

48.0

139.4

40.9

73.8

141.2

255.9

28.3

54.6 52.6

135

3

1&

1044

38.0 44.5

52.1

1314,6

26.5

107 -I

334 53.5

1134

39.9

554 56.2

151.2

49.1

74.7

19.7

173.5

39.1

74.8 33.3

1147,2

28.5

94.0 57.3

179.8

5 106.4

464 50.6

71.4

168.1

47.6

59.1

2E1.4

135,1 604 62.2

454

1684

6 109-1

7 112 -I

43.3

86.7

45.4

175.4

21.6

60.14

37.1

119.1

31.5 38.5

21.8

91 8

37.7

95.9

49.8

183.4

30.9

82.14

60.7

MA

214.6

58.5 60.3

140.4

8 123-I

514.8

80.2

145.2

180.2

66.1

51.0

28.7

1145,8

78.9

69.0

35.5

183.1

9

10

11

12

19144 35.9

43.4

214.5

103.8

59.6

46.4 30.5

136.5

36.3

55.4 32.0

123.7

127.4

21.4 30.7

39.9

,2461,

46.2

25.8

29.7

91.7

29.9 13.7

36.2

1324 39.6

71.0

52.9

163.5

33.1 52.5 34.6

1204 264 4640 woe

404

112.3

135 -I 27.1

147.8

344 109.3

26.2

53.9 45.0

3254

23.7

52.4

30.2

106.3

13

138-I

31444

27.2

14 139 -I 31.14

184

7967 48.9 43.0 21.6

113.5

35.3

434

27.11

106.4

604 37.7

129.2

28.6

624 47.0

137.7

25.2

524 58,8 136.2

15 1444 35.2

71,3 26.8

133.3

23.1 60.0

194 102.1

48.8

59.0 18.6

1264

16 B.G.

29.0

51.14

19.6

1004 29.6 36.7 55.8

122.1

2841~

45.0

53.4

126.8

81 612.7

951.6 629.5

2193.8

606.4 898.9 590.2

2095.5

572,1 885,1 663.1$

=2

214973163

28392.03

62244.68

317999.50

25566.36

21425.214

54840.39

28151103

23938.39

30312.4

530249

291783.86 26360803

1

83.9 7

55.8

195.6

188.6

132.7

243.5

171.9

185.6

236,8

200.2

7 71.0 47.8 *7.2

pt!

53., 143.0.

7 46.0 52.4 43.7

2

169.5

154.1

113.9

71.3 51.4

60,0

59.0

36.7

1904

4

24.3. 41.2

374 52.6

524 49.7

7144 115.4

53.5

33.3 28.4 37.1

06.2

574 45.2 21.8

49.8 45.2

60.7 28.7

60.3 35.5

39.9 52.

30.5 29.7 346 1154

32.0 36.2 40.2 30.2

184 37.7

21.6 47.0

27.4 58.8

26.8 19.6

19.0 55.8

18.6 53.4

75.8

141

161.8

140,3

1454

104.3

170.8

109.14

87.0

105.8

127.7

109.6

67.1

343.5

64414

128.8

11/8)A

407.3

430.8

398.9

500.5

471.3

386.3

497.8

509.4

364.0

2$.5

340.7

299.6

403.1

361.8

348.9

62147301 54687.89

74413.97

93382.92

46023.28

547 5.95

31541,58 51831.86

65757.36

92595.23

54618.81

91968.1414

25889.93

41687.97

58266.65

14417)4,05

14237970.41

Method)

2.03-

107.1

100.1

109-1

9

10

11

7

8

5

6

1214I 3.2

127-1 13

132/

14

135.1

3$

138.1

16

139.1

17

144.1

18

1

2

3

5.84

5.78

5.02

3.27

1.53

4.65

3.20

3.140

3.76

8.1,

5.$2.

4.28

8.55

7.59

2.83

7.25

7.814

2.95

3,10

4.20

3.19

445

2.88

14.114

4.83

4.39

4.80

4.99

5.18

4.52

see 112.1, X.Y.T., below

7.98

3.46

3,08

4408

3.90

5.36

5.87

1.49

4.32

3.58

3.64

5.62

2.75

5.48

540 5.09

2.01

241

1.42

3.92

2.78

...

5.73

2.84

545

405

1.72

5.08

3.51

3.19

112 -I

128.1

* Fro* itepello

5.07

8.99

5.26

9.17

674

9.36

4.69

8.73

19.76

36.25

2, 3, 4 or Experimental Yield Trial

23.06

16.85

21.16

12.54

6.16

18.97

13.07

10.23

17.67

1,5

31.97

154.43

9.24

22.25

16.57

17.92

20.05

9,06

5.76

4.22

549

3.14

1.54

4.74

3.27

3.41

7.71

3.06

3.06

4.14

4.48

5.01

a Cis

15

16

17

5

6

3' a3.1ion

104

1044

7

8

9

107 -I

108-i

109-I

1124

U

1234.

12

13

12144

127.1

132 -I

114

135 -I

1384

1394

111144

1284

.93

2.94

2.3$

.73

1.11

.80

.85

.96

.96

.22

.75

.97

.52

.69

2.37

.67

2.49

2.61

.83

.65

1.06

1.03.

«65

1.28

.92

.73

.90

.22

.83

.65

142 835

47* 1.57

1.23

.88

.66

.86

.83

.65

.67

16

.30

1414

.33

514

2.03

a-

2.3.0

142

.64

.52

.59

2.15

WOW 0( than others Probably nixed either r sampling.

Not used in avg.

T.T.,, hills 420 & 4.

0,59

2.60

243.

0.78

1.17

0.83

0,49

1.30

0.60

0.75

045

0.914

0.21

0.85

0.64

0.10

0.62.

2.1,6

the Corvallis4Proosor Tiold Trial, 1958

138

12 I

Total 34

2 51

3.3

8#

3.2e

15

35

0

18

14

19

21

0 0

9 0

33 21 n 31.

32 21

22 14

14 17

3 51

8,

148

52 Total 63 54

11

U

0

90

15 25 35 39 121

Total

37

43114 73

14

5'

17 0 7 0

8' 30 15 0 9

12# 36 32 39 22

Total 83

147 146

31

5 51

21 6 (6

8'

29 31 (29 (31

12'

33

41 (33 (la

Total 83

78 83 78

6 51 0 o 5 0

8'

7 3.2

32 13

12' 8 12 25

U

Total 15

214

62 24

7

5' 39 0 0 31

81 24 13 11 24

12' 24 19 10 23

Total 87 32 23.

78

8 51 0 0 15 26

81 21 20 0 36

32t 30 31 20 26

9

Total 53.

53.

35 88

5'

12'

1

0 0 0 19

11 6

10 5

Total 21 11 33 37

Rep.12

3 4

Grant

Total Total

8 0 0 6 8 r

6 0 12 23

21 18 10 50 26 104 17 37

66

0 7 63.

30 23 34 26 93 38 32 36 10 116

39

186

275

95

50

103

114

28 33 90 54 205

6 0 0 0 6

47 21 33 16 117

40 33 41 27 141

60 75 36 29 200

8 0 0 0

20 15 30 4

8

69

30 23 40 27 120

500

60 33 3 6 0

70 14 22 18 13

87 27 18 20 9

42

74

12

57 13

12 n

22 1.8

7

13

11

24

142

62

34 20 74

344

189

235

568

217 74 33

144

22 173 47 52 34 45 178

64

289

375

728

267 93 54 74

143

264 58 38 70 31 197

24 29 5 25

54

129

0

29 10 20 9

59

68

26 16 32 145

119

12

8

8

7

15 20

17 26

55

58

26 32 40 51

1149

138

180

397

715

207

54

120

148

814

31 77 54

2146

214

0

14

0

214

0 10

28 35 11 13

28

34

87

46

147

72 97 262

36 12 0 2/4

72

26 22

34

16 33 97

29 20 24 107

322

5

614

56

52 59

13.

27 149

0 0 o 36 36

4 15 30 25 74

21

214

17

141

103

96 63 36

83.

276

0 0 0 0

20 12 13 24

13 9 20

142

0

69

814

125 25 39

147

102 213

70

72

76

0 8 0 33

13 30 10 16

18 28 9 21

33 21 33 66 153

43.

26 38 0 34 98

69

3.2

76

20 16

3.9

19 25 17 38

67

99

218 31 66 19 70 186 57 83 33 91 264

Ia.

77

107 o 0 0 0

140

143

0

18

22 29

0 15 73

24 118

0 0 0 0

25 214

32 14

23 32 13 16

0

95

84

225

12

_.5

83

140

29 39

2 2

8 z 8

19 18

12

20

10

22

191 48 56

145

30 179

41

60

9

12

9

1k

13

.0

10

18 22 62

102 19 18 51 50 138 30 33 29

143

135

154

251

342

7147

41

207

243

1493.

209

208

251

668

41

245

309

595

167

375

loz

10 51 es

121

0 15

9 45

29 22

18

37 lb

Total 29 82

69 75 255 71

10

( 3

18

71 18

11 51

8'

12'

0 0 37

32 10 19

17 29 43

0

17 11.

6 47 42

6 95 33

8

0 0

11 12

5

26 15 17

Total

29.

39 79

225'

0

Total 18

0 0

8'

10 12

0

12'

8 14 20

12.

159 86

5

12

12

26 20 29

54

93 31

145

42

27 22 180 o 0 0

10 17

14 8 49

21 25.30

6 82

44

3.14

131

0

27

37

9

8

6

0 0

5 5

7 10

13

29

37

13 51 0

8' 6

12' 8

0

8

10

0 0

6 7

13.

8

Total lb

14 51 9

81 28

12' 19

18

9

18

38

17 15

22

36

0 27

33

18

Total 56 65 58 78

155'

0 0 8 0

8'

12'

10 10 10

9

15 12

12 12

Total 25 22 30 21

64 23

45

101

22

31

Ul

26

257

26 8

27 28

20 34

79 73

30

36

38

70 104

8

39

51

98

12 15 29

6 6 22

9

14 11

9

29 26 26 13

44 46 59 22

79

86

122

118

326

314

143

94

171.

23 30

30.101

146

94 271.

0 5

18 12

21 32

0 o 5

17

17

20

35

67

105

39 49 34

55

0 o 0 4

20 11 17

26 19 22 18

9

177

14

57

85

46 30 39 31 246

0 0 0 10 10

8

9

16 14 15

22

53

16 14 61

17 38

7 27

20 23.

15 26

42

0 8 12 4

13 7 9 7

24 5 5 8

37

30 39 124

26 10 70

36 24 101

39 20 100

74 103.

54 271

214

36

42

20 26 19 102

704

184

291.

516

9

140

221

370

23

109

135

267

201

324

329

854

66

118

187

371.

16 51

14 17

8 6

8'

33.

18 10 11

12'

8 6 4

214

45

70

42

24 17 10 22

29 26 23 19

19 29

25 21

73

97

94

10 13 16 0

27 26 33 13

29 39 33 18

39

99

119

157

266

255

Total 53

111

22 41 3.57

72 72 58 62 264 66 78 82 33.

257 678 sx 698 653. 759 753 2861 895 681 786 732

30914

752 858 746 836 3192 9147 sae

ST2

15644 19643

15359 18427

214949

20480

14839 18166

167914 19220

21662 22102

39880 147827

33339 146333

601467

60793 47250

34223 45208

652556

140362

42170

514432

2192805

53764

690040 5650499

L.C.

143.4

104-1

107 -I

108.1

109-1

112.1

123-1

124-1

121.1

132.1

135 -I

1384

139.1

144.1

sX sx2

156

3.324

1095

1323

2086

3.140

1533

1911

1156

1 060

749

3256

987

1876

1805

1

21159

1951

Data from the USDA

Hop varlets]. Trials in Cooperation with the Irrigation Experiment Station, Prosser Welk.

(tram C. Z. Nelsen)

Pounds pe

RsPa

!tail

.!!2111

2212

1953

720

1636

1820

778

1492

1305

1194

920

921

855

1350

1393

2530

89k

749

11156

1538

2120

1080

3315

1213

( 495)

736

350

894

2h34

1302

2009

1369

824

1417

2258

1012

1035

1.302

845

100.3

502

1124

1598

1327

1.558

1728

690

1305

1902

1449

1437

2614

2437

251

928

1069

941

2578 lobo

--EA

1211

2049

1454

814

1427

1921

1100

1315

1681

1169

495

869

620

960

1767

3 flowering Debi dab' ar"4444

18967 17066 17956 20027

157543 27537797 23224328 254308i2 34952723

6881 911,771

* Not harvested*

Fertilised Mar* 19-22 2 t. alfalfa/a plus

Banded 120 1b3.

immonlun nitrate*

1958 Data from the USDA Sep Varietal Trials in Ommummties with the Irrigation Experiment Station, Prosser Wash.

(from C E. Nelsen) acre

11.22.1.4

La.121

LI/

Flamering

Lira

L.C.

103-1

104.1

107 -I

108 -I

109-I

2432

1879

954

888

1659

880

112-1 870

123-1 1789

1244 691

127 -I 328

1324 783

135-1

138-I

685

868

137.1

1252

144-1 1006

3018

2388

865

842

1653

1484

1231

1699

1057

382

1.141

830

693

1382

1042

2872

2118

850

1042

2097

996

1099

1989

1045

461

1101

643

993

1177

1170

2741

1991

970

1137

1673

880

964

1623

1503

333

1203

824

1567

1042

1351

2593

1556

822

902

2370

1906

1075

1912

1049

389

1175

832

558

776

1592

19653 20,302 19727 SI 16964 197©7 a

2

23628370 32334575 31.766393 32311122 31857593

1081

763

936

31

1332

Fertiliser side dressed in

14 bands 70 deep April 10.

100# Eta as ammonium nitrate

101 ZN/a as situ) ammonium sulfite

671 P201.

as treble superphosphate

Irrigated every 2 weeks

1234

12144

127

132.I

1354

138.1

1394

114144

L.C.

1.1c7

3008

4.14

3.05

1.95

1.90

1 .87

2.75

2.145

1.73

4.78

1.77

78

2.27

3.214

5.22

3.00

2.18

1.82

2.11

2.68

2.22

1.83

3.06

2.68

2.91

3.146

628

680

1.57

1.61

1.55

2.87

2.49

2.81

14.811

0.66

9.78

13.914

10.85

5.70

5.33

5.53

7.30

7.16

6.37

12.68

2.89

3.26

14.65

3.62

1.90

1.78

1.84

2.43

2.39

2.12

14.23

107-1

1084

109.1

1124

1234

124-1

127-1

Me

Bud

L.O.

.29

103-1

Al

1044 .28

132-I

135-1

138-1

.91

ash

.30

.32

.15

.40

.19

Ai

.51

.52

139-1

11414-1

.35

AO

Oil Coated': OorvolUisowtrosser (Prosser), tAtisACO pest* bow tby bads )

.26

.Lit

.30

.147

.29

.32

.21

ftti

4 fowl."

.29

AP 46

.25

.25

.12

.35

.15

.29

.63

.89

.21

.22

.39

la

.87

.37

AO

.20

.36

.52

.se

.33

.29

.15

.28

.51

.22

.1a3

31

.31

.18

.33.

.49

.29

.29

.145

.38

awes al Variatifa ate., Corvallis

15

Total

30

47

167.749

3,437.411

8,953.543

114.5)30

Linos

Reps.

Error a

Years

Limonx rears

Grand total

2

30

64

143

15

2

30

3

108.688

1082 961 1.36.099

11,299.259

5,649.630 *I*

10,731.771

357.726 **

6,746.423

105 413

40,550.525

Lines

Error

Total

15

2

958:

11,

02.0

301.1

558

575

70,025.6

32 028.7

780.13

41*

2.50.55

125.49

Yield (lbs./i), Prosser,

19572

Uses

11:

Reps.

Error

Total

56

74

280

864,991

5,243,857

2(4234128

1,008,734

41*

216,248

93,640

rita1 its4ressrp

Analysis ot

%tom

JE

seri

1958$

Ilt

56

711 l ,278,325 4734166

461,945

114146

72,42

28,712

60,222

**

lei. all per

MR Ai 4.11,

10 ma

2.$ «

14 *

*Pro bla0 Iielertaa

0.714 dry

0.94

%OS last

Goli sang* 51,

1t.37

128-I

135-4

MIL

4"44441

6.75

206

5.00

5.70

6

5.26

514

Backe

B 0.

L.G.

103-I

1074

108-I

112 -I

1351

139-I

114-1

Alpha-eeid Corm of Via. Brom (1958

(%a( said, .was, Colors metric 'frothed angle replication. 5 hill plots

5.63

8.014

8.03

2.20

4.214

7.09

6.24

2.63

14.27

3.52

Sacramento

County Hop Genetic Block, Bradley Ranch

Readings taken August 11, 1958 one week prior to harvest

(data from lift. 0. Golden, Jr.) h 8, 1958 - Flooded three times before emergenosi

Vines per Milt Number of vines trained on three strings. 0 means hilt miseing, replanting necessary

Average arm lengths Estimates in feet.

Relative puma yield: Readings based on Early estimated b plants in alterneating yield 7 -9 bales per acre.

Readings based on Early Cluster baby plants in alternating top-set and very adequate middle and bottom met.

NO positive mildew symptoms rated in genetic blook; field heavily infested during most of season.

within 1/14 mile wile

Genetic materiel to be hand harvested and replications bulked ecoording ter select/am'

Hops to be forwarded to Stan Brooks, Oregon State College.

Vines

r

1

2

3

It

5

6

4

6

0

0

3

3

1 -3 x Fa-5) a a

I

1

2

3

4

5

1

In

2

2

0

0

0

5

0

2

0

0

0

4

0

0

0

1

2

3

4

5

1

2

0

0

3

0

0

14

0

0

0

2

4

3

1/23

Relative gemsral zield

(Bel 31.3 x

2 -3

3-4 1-2

G x Bel 31)

P

SPind2.7 plant still SA %UV

Small cones, lets,

3.

vi numerous browsed airso nigh (spikes?) felimssiztlaigifgft.

Rep.

It

1,

2

5

1

2

3

14

3

2 p.

1

0

3

0

Aw r. a lent c

(13)

Relative general

yield

Cone distribatim

noel to

.

6 ft. t.

mire

112 Sam x (Bel 314 x Bel 31) sent.

1-2 312-.1

P P P

411401OmmbleOwt set below 6 ft.

Spindly plant. Wm arm & vines

2.3

4

P

P

Red vine, dart green foliage.

2 ft. below wire

1

2

3

5

1

2

3

0

0

5

1

4

0

1

I

0

0

0

1/2

3

2.3

1.2

3

3

P

0 a

X x U a

P1

P

Rep.

5

1

2

3

5

1

2

3

5

1

2

3

14

3

5

1

2

0

6

2

5

14

5

2

0

5

0

0

2

0

2

6-7

Avg.

1/2

6

Relative general.

J4

135 -I Fu x (R7 a

Unbmown 3)

1/2

3-4

2 -3

COPRIERCIAL INIERPIARTS (La

3-4

None

0

(7 139-ix Barr x (Bel 31.4 x Bel 31)

3

2-3

3

2-3

2

3

2

1/2.1

1/2-1

2.3

2 -3

1-3

1-3

1-2

1-2

1-3

0

P

K

P

N

K

P

K

K

P

K

PI

a

K

ANIONOWNIONIVINOW.

6 tt

X

0

P

Vigorous plant

A tow lemma blare

1 vino not to wire, e tbors just, to Ids*

Some burrs

la

5

1

2

3

1

2

3

4

5

1

2

3

4

Hill Vines

No.

per $1/1 arm len ground to

6 ft.

(8)

6

0

2

4

4

1-14

4

14

1/2..1

3

0

3

2

1-3

1-4 1/2.1

0

2

5

3-4

2.3

2 -3 1

Relative general

Yiald

Cone distributicn ground to 6

6 ft.

mire io

P

P

P

P

P

P

P

N

N

P x adjoimmg) adjoining)

1

Early Clusters adjoining)

155

STANDARD FORM

MEMORANDUM OF UNDERSTANDING between

Name of Grower

Post Office Address and

The Crops Research Division,

Agricultural Research Service, United States Department of Agriculture and

The Agricultural Experiment Station

The purpose of this informal cooperation is to test promising selections of hops.

The plant material noted below is furnished the Grower and accepted by him under the conditions cited in this memorandum.

le The above cooperating agencies agree to furnish to the Grower the following plant material:

2.

The Grower agrees to grow the above plant material in the following location(s):

3.

The Grower agrees to furnish the land and labor and meet all expenses involved in caring for the plant material and the harvesting and disposal of the crop.

It is agreed that the Grower may dispose of the crops as he sees fit, except for propagating purposes.

Representatives of the above cooperating agencies are to be permitted to make observations in the field from time to time and to obtain samples and propagating material from the plants when desired.

4.

The Grower further agrees not to propagate additional plants for his own use, nor to sell, give away, or otherwise distribute any of these selections until authorized to do so by the above cooperating agencies.

156

5.

The Grower agrees not to dispose of the land where such plants and/or propagating material are growing without first destroying such plants

2 unless the purchaser becomes a party to this agreement.

6.

The Grower agrees to permit the representative of the cooperating agencies to destroy this material if it proves diseased or off-type2 or if it is discarded.

7.

The cooperating agencies will furnish plant material believed to be free from serious diseases or insect pests.

The Grower further agrees that he will not hold the above cooperating agencies responsible should insect pests and diseases appear on the plants.

8.

In the event that the cooperating agencies decide to release any of the selections 2 the Grower agrees to distribute at least 75 percent of the cuttings for increase purposes to individuals designated by the cooperating agencies for a period of two years following the decision.

It is further agreed that the

Grower will sell such cuttings at a price determined at the time of release by an allocation committee of the cooperating agencies.

9.

No memb er of cr delegate to Congress or Resident Commissioner shall, be admitted to any share or part of this agreement or to any benefit that arise xherafrom, but this provision shall not be construed to extend to may this agreement if made with a corporation for its general benefit.

10. The coope-:°ating agencies designate the following representative as coordinator between the zooperating agencies and the Grower.

He will be responsible for the distribution of plants and for directing the evaluation of the material

Name

Address

Date Grower

Date

Directory Crops Research Division

Date

Directory

Experiment Station

Agricultural

desired plans testing of enpsasinoiatal help varieties in Orogen.

San may be necessary duo to liaitstions in trellis sise or shape

Any sodifiaatifens that do appear to be nee*

*red sines they may reduce the precision of the trial. Otherwise of fertilisers tivation, spraying She use will be consistent with the pCraetWoes in dement use by the grower-coo

1. Varieties to be tested*

112 I (0 19215) Sam z (Del x Bel 31)

128 I (C 19113) Du z (Sioux Unknown-5)

135 I (C 19151) Fn x (RV x Unknown-0

114& I (C 19077) iF z (1,0,xyg.$)

Tuggle'

19209)

2.

Planting stocks

A minimum of lop hills of each riety will be rem red in o sufficient hops will be available for large-scale brewing trials.

Planting stock of these varieties will be provided by the Collage under a mismoraNhalof understanding between the grower and the college.

3.

Fieldiplot diagrams

The locations of the varieties in a trial will be deterrined at random.

The plets will consist of single roue grown side by side with each series of the fire varieties being located adjacent to another series or replication.

The planting of border or guard rows will be permitted around the entire experimental area.

Guard rows may also be grown between the replications, but not within replications.

I 48 should lie I,aisi oat sash saw that theiir disessiisli a of slops Sr other Nil gisAients. Stringing and training should be dens in a manner whit% feeilitates harvesting of single plots ens of the following plestisg plans may be used

!bra particular trial (plots namborwitrem the leftleben teeing the trial from the Meese roadway), When three replications are used the plots 'mill be 35 bills lion0

When twee replications are used the plots will be 50 bills long.

!mg

Plan I asp= RepIA

103

0

I

102

201

205

202

Plan la

Repa

Variety

H22,14.

112 1

128 1

135 1

144 1

Tuggle*

102

101

105

103

104

204

205

201

202

203

305

301

304

303

302

Plan III

&spin 11.14'4

Variety

1121

328 1

1.35 1

144 I

Tuggle*

103

102

101

105

3.014

205

203

20k

201

202

305

301 ing and dryings is

Harvesting of the experimental plots is to be ac equipment of the grower-cooperator.

A college representative will be present at the time of harvesting and drying to assist in beeping the yields from the individual plots separate until a measure of the yield has been made.

He will

The also obtain samples at the time of harvest on an individual plot basis,

the drying vaxi

y'ney bar semosited for drying if it le

balm simples are is

will be nemseary to keep the varieties

s ism order that they can be

Wad separately

Data to be obtained,

The min *bleu, ram y, disease mistiest and picking ability. A minima et tefOre these cheviots stics of data (ii years actual) is ooneidered yearscan be determined with any degree of ee ye

Yield will be determined on an individual plot basis by co ting.or

'sighing the sacks of hops ebtained from thin. Samples will provide information og dry -down for adjusting yields to a demon moisture content.

b.

Quality will be determined by laboratory analysis of the samples provided on an individual plot basis and by brewing trials.

Baled lots of each variety will be provided fray one, too, or three trials each year Shich will be submitted for brewing trial analysis.

c.

Disease reaction will be determined by observation throughout each season in conjunction with more detailed studies at the college do Picking ability be determined by observations on shattering, leaf and stem content, and ease of picking at the time the plots are picked by oommercial equipment.

The release of any of these experimental, varieties to the hop growing industry will be determined on the basis of their performance in off station trials and their acceptance by growers and brewers.

6

SI

2

Treatments

Replications

Error

Total

30100

70

II

.4

17.3

24.9

23.0

28.9

32.2

342 33.4

244 20.9

27.3

16.2

21.1

24.2

"25.6

22.3

21.7

15.6

24.9

20.0

.1

109.6

131.7

11403 11769

2892.95 2576.34 3523.91 2777.29 46346.67

720

1070

750

760

730

4

3

19

MS

77.542

53.546

17.249

130.876

10.906

494.590

SI 20090 3084 362.8

328.7

311.5

354.9

#95.7

liar

8554.54 19969.47

27U0.50

22299,37 2062941 256117.39

3.8605.63

2162.5

96809

Irrigation levels

Replleations

Error a

Subtotal a

Fertiliser levels

I x

Error b

Subtotal b

6

24

90

0

4

3

12

19

1,1,6 292.3975 *

273.467

91.1557

690.625

57.5521

2,133.682

872.672

269.738

11.2391

4033.896

11.4877

4176.306

4.309 988

Note: The harvest wts

n in this and the precedi

on the basis of 25% dry-doun before moisture data were a dry-down was later determined to be 28.6%.

table were computed b The average

The yields in the to ch, in pounds

per acre, take this into consideration and are the correct value

(adjust x 1.14).

rrC

t96,1 aCC

6St

9rc

60*6E rtr /C

ErE

/ot!

Won

99C /nor i

5

Wat

92411

sori Ern

Cr at

Lot

CrE

WC z

Lo£ 69a

Sr(

aim' lir a

LE

ic

Cz

LosE 5£C trier

C

-NM

IC 91 tirCI

8L £t

VITT

05C 99C WC tiLC

Ma

Iraq IrrE

Or( WI

99TE

59°C

90°C

99°C

6r

E

ITC

/47* a

95C't

CL trc

Wirt

arr gEC

orc

sec or £

9PC

Irv(

ETC 59C 'Tr(

9rc

oc cri scc

II

C t a

ETT

tat

Plrivintott irialP6)00 a col/41-Pi so asTIPAA t

9e6 I a

C

0a.6 lrot

9o01 oot aPot

56'6

9

9r6 a

In6

09.6

Te6

Trot

'Wf

Ce9C nC.6C

8911

wog

Teo

/*ol C9651 evaltmay

Jo eaurralm v$TaaT uo ILI:rm.zos °P1-0,)0

21T.2.11 de% u)444:51

(x)

344g

(a). scan) *

(alca

IanTI

LOW

9910

9C9aC

Lo9Tt

90agn

610005 a

9

9

La,

Ln raC

Ober

9a05o

Mar

StiC69te

LLot64111

**

29C

LooSo

165 t.

oil per 100 grouts' boas dry istsis)

A

1

2

3

14

0

0

0.145

0.42

1.76

F.Total

5.66

Mean 0.147

0.0

0.38

0.67

0.60

2.20

0.1,0

0.33

0.49

0.148

1.89

041

0.56

0.50

0.148

2.05

6.50

0.69

0.59

0.83

0.65

2.56

thit7

0.55

0.39

0.148

0.514

0.55

0.60

0.72

0.52

2.39

0.0

0.66

0.43

0446

2 Alt

0.148

0.66

0.57

0.57

2.28

6.71

0.56

0.145

0.54

048

0.67

2414

6.62

0.55

6)0'02

0.67

0.614

2.58

0.48

0.42

0.148

0.52

1.90

0.61

0.147

1.89

2.20

2.00

2.14

3.23

25.149

0.53

a

C

Ort

srt

Tin

°PI

975 met

art

099

9

WI ort

Crt

crt a9'9

Owl

Cet

't

SS

11,*9

Ma&

'9

Z£'9

re9

6r9

Jo a

Armiros-tramoswi

-

Wvoloo

96eLo floa00°

MO'

C

9

LZ

9C

Lit

ono

/woo

WS'

69

*

3400 i

1 reaching WOO 274

5400 ppm reaching

812

27,6

6100 9pm Willi flower 23.0

7400 pia initial flaw 254

SI

185.2

182.8

2795

20,0

19.4

12.7

26.8

16.4

1762 141.0

5339.96

14907 58 4637 02 298902

960

10

990 99.7

81.5

75.7

6834

75222

750

Source of variation

Treatments

UP*.

Error

Total

6

3

18

27

178.709

86.3292 P < 10 *

59.56911

32.6509

* ir

2.614 reclaims 2.66

5%

WOO sp

woons tirr"ppowntrociao

uo isaktimu

sot

891:

*DD

411

03 001 Rild '44.941 I*9 c

00 otod 9.11 q; eti 001

1014 t-ot

9

1.01

'5

005 'Add

31-0T 61;

9'

09 pa

Iimpewcid 411:Plant L'11

'L cos

TiTvrtretwdet 21t-Tasmtou Ssot

Is a

Is ri tr9

65

9*

5'01

trot

III

rot

$*5

6*$

re a

1.°

am

96 56

Al

eat

0"9

C'$

re

L 1.

9'6

()on

WU

zon

114

rot

09 tirri r%

6C L

roil

1165

o'6

6'6

L'Ot

rot

1.19

VT9

90

595

9.1.,1Z

$9CC9 W095 10*(29 65'915 999ee6 soxaog

O uowirraweA

Bizeuroon sumirortdou moan

Troy

9

VT

La

Tit notts6t

** tC6 nest

2

3 500 pp at 114

ft.

37

It5

Is 100 ppm at 10.12 ft.

5 500 ppm at 10-12 ft.

32

6 100 pm at lit bar,

31

7 500 ppm at lit bum

30

2117

8859

311 ha

1e6

32

311

29

31

237

214

31

31

2/42

37

31

54

9382

123

980

13900

314

35

Source of variation

Treatimsvbs

Replications

Error

Total

6

3

18

27

99.9

700.0

5.55

(ixt.) in the Gibberellie-aeld.

Treat, 1 2 th

Total 1

Rep.II

2 3

14

Total 1

Check 141

71

0 0 0 0 0

10' 6

.1

5 e

2 -20

0

U

10

15

0

114

0

17

10

4 9 7 6 26 10 13 13 10 46

57

6

8

7

Total 10 16 12 8 46.

23.

38 27 27 113 21

2

141 14

0 15

214 143 8 18 8 0 34 36

7'

9 8 23 37 77 14 14 17 10 55 31

10' 11 9 16 24 60 13 19 15 13 60 33

Total

214

17 54 85 180 35 51 40 23

19

100

Re .111

2 ,3

14

16 0

'0

20 7 7

17

6 11

53 13 18

0 10

9

17 32 1.2

23 15 31

140

37 52

BaNIT

Total 1 2 3

22

42

41

105

55

72

102

229

7

114 lo

11

39

0 5

10 9

9 3

19 17

23

10

10

8

7 a

I 13

28 3.6

52

0

7

9

3 41

71

5 7 25 23

21 8 23 17

60

69

0 II 0 0

9 3.5

11

4 16

0 35 25

10' 17 13 29 24 83 15

111

23 18 70 27

Total 43 28 77 64 212 24 33 34 18 109 68

41

7' lit 19 17 8 58

18 8 12 1.6

514

0 15 7 3

3 9 12 16

25

140

0

20

10' 12 10 12 19 53 10 9 10 13

142

15

Total 44

37 la h3

145 33

33 PO a

109 35

5 41 0 0 13 0 13 0 7 25 20 52 0

7' 12 9 18 7

46 19 16 21 30 86 18

10'

27 31 13 9 60 12 10 25 23 70 20

Total 39 20 44 16 119 31 33 71 73 208 38

8

17

27

52

72

0 0

13 7

26 la

39 25

9

12

0 0

10 17

15

114

16

$ 11 -011

33

22

1.7

10 6

23 20

24 24

57 50

24 11 0

62 19 8

98 33 10

1814

63 18

9

0 0

59 0 15

60 21

114

In

21

149

83

8i;

0 0

7 13

13 23

217 20 36

0 0

9 18

8 11

17 29

5

19 19

21

214 igt ILI 1,38

0 0 0

11 18

4.9

16 16 41

27

Total

34 117

632

Side n h (in.) in the Gibberellic -acid Trial on Fuggles, 1958 -- pouts

Rep.I

Rep.II

Rep.III

Reptr

Treat. 12 3

4 Total 1 2 3 4

.

Total 1 2 3 4 Total 1 2 3 k

T

6 41

7'

101

0 0 0 (0)

4

8

0

4 13

(7) 28

10 12 (10) 40

19 17 0 14

8 14 13 12

14 19 12 12

50

47

57

36 0 6 0

13 8 11 7

18 13 10 21

42

39

52

0 15 6 0

13 11 10 9

10 9 14

8

Total 12 14 25 17 68

41 50 25

38 154

7 4'

7'

101

14 8

28

27

6 0

14 21

8

28

71

17 13 26

73

Total 69 39

40 24 172

0 11 0 23

22 17 19 14

14 19 22 17

34

72

72

36 47 41 54 178

67 21 27 18 133

0 24 0- 0

17 21 17

27 27 17

19

21

24

74

92

44 72

34 40 190

23 35 30 17

0 0 0 7

15 20 12

14'

24 22 6 13

7

61

65

39 42 18

34 133

T

41 171 293 257 962

201 285 267 265 1018 373 346 231 236 1186 196 216 188 225 825

4351

1949

5277

5235

2871

4221

4735

4743

9261

7136

3735

4258

3646

3230

2302

_3621

10887 14831

4815 14355

155054 12021 12295

6349 11673 156964

24159

19218

8769

9166

214584

7260

5580

8251

Alpbameold Costomt, Oibberellia ANSA (Mg)

(%o(.astalo Detre 001021ailirla Illathad) a tral

110 ppa 449 high 2

1

Ma biz& lea ft:it

Miati

328

4.06

441 448

3.65

3.94

4.Cts

4.39

500 Xs 3 4.38

448

642 4.24

110 PM 10.

500 P a w

4

5

4.014

4.60

4414

4.17

14.41

4.28

4.36

3.62

100 ppm at burr 6 3.64

3.51

3.62

3.64

SOO ppm 7

240 2.69

2.64

2.63

17.32

16.87

1645

14.41

10.76

kW OP

16.23

4.e6

1642 4.0 0

4.33

4.12

4.1,

3.60

2.69

26.39

26,79 27.70

27.48

7148,36 3.87

Treat.

Esp.

Error

Total

Analysl.o of Variances Gibberollia Acrid: .0(-021A.

SS

7.8021

.1578

1.7890

9.7489

6

3

18

27

1.3003

.0526

.09939

13.0828

4141.

A.S.

0 Sew« hill

D Mod. hill

Pled 14/25

SI

sit

23.6

22

23.2

16.2

20.2

21.4

26.4

234

2140

234

17.1

25.2

17.6

28,6 21.9

23.7

22 26,0 25.8

24

28,9 22.9

21.8

15.9

32.1

16.6

19.6

181:4 830

19.3

1734 800

19.9

30.5

25.6

31.1

1940 !1©

11}.4 117.5

1014.9

118.8 110.9 13344 109.6

123.6

933.1

2656.96

2800.37

2293.31

2845.26

2552.23

3640.68

2463.84

17h,50141

3170.02.

Analysis of Variance

Source of Variation

DE

Treatment 4

Repo

Error

Total

28

7

39

SS

45.75

11.44

3.12.02

16.00

498.01

17..79

655.78

150 Mat

26.0 112

23.6 108

23.2

98.

211.0 1090

17.6 89.7

38.1 32.5

4440 33.7

53.3 35.5

5440 3514

46.1 43.2

22.4 9344

22.2 99.9

20.2 209.0

23.T113.4

24051110

35.4 28.3 19f

.5 23.4 164 724

.5 33.4 ask 980

.5 31.6 174 89.2

310 21.9 98.0

221,5

183.4

114.4

519.3

235.5

180.8

117.5

533.8

.6

155.3

latiot

1465.8

9909.99

2656.96

1126901

6777.50

5429745

2800.37

6607.24

57389094

8557.60

2293411

4905.89

441 8

Rep419

A Se

8 mod#4/11

C seve4/11 g 26.4

32.9

39.4

47.3

47.5

42.4 20.1

304 26.4

33.5 23.4

404 25.2

27.4 23,7

88.

904

96.3

112.8

98.6

sic

Bop."'

R.P.VI

32.3 140.6 23.8

51.4 34.1 22.4

45.4 37.1 28.9

49,4 32.5 15.9

33.4 29.8 19.9

96.9

37.4 36.3 21.9

95.6

107.9

44.0

310 26.01.7

111,4 45.3

37.7

22.9 105.9

97.8

56.9

)9.9

434 434

143.9

32.1

1200

30.5 117.4

193.5

1744

1124

486.8

211.9

174.3

U0.9

4974

226.6

189.5

133.4

549.5

7825.27

2845.26

6249.67

47758.74

9302.33

2552.23

6148.15

10473046

49912,43

3640,68

.

7263.09

61095.03

A No Pruning

B Mod

C Swv.

D Mod.4

Mod.4/2

Rep.VIII

45.7 45.6 19.8

54.1 38.7 25.8

48.4 41.6 21.8

60.5 43.1 16.6

414.45.9 25.6

111.1

40,5 38.1 29.0 107,6

118.6

53.3 37,4 214.6 115,3

111,8

120.2

41,7 38,4 19.6

99,7

41.0 46.0 19.3 1014

1134

9.4.1 50.0 31.1 13205

Treatment

Total

813.5

014.5

831.5

879,5

850.6

Si 250.6

2169

109.6

575.1

228.7

209.9

12306

562.2

4189.6

SI2

12773.72

2463.84

9272.03

10609'23

66216.01

3170,02

8940.93

63838,60

3

5.36

33:9

33.9

34.6

36.6

35.4

Error b

6

70

610.329

564.789

11.557,29115 .*

76.2911 ** soak

12,2254579

Treatment z Tears 2-wy table

A

B C I)

IL

1956

1957

1958

EL

SI2

3094

311.9

191.7

361.1

266.2

187.2

361.9

288.2

18144

397.h

308.2

173.9

3143.6

308.1

198.9

813.5

81144 831.5

879.5

850.6

230,068.51

236.299.49

2146,936.81

283,155.21

252,5147.78

Treatments

Reps.

Error

Total

Analysis of variances sidearm lengths 1958

14

3

232

239

1,476

779

13,238

114,993

MS

269.0 **

259.7

56.6

Total

1,773.9

1,1482.6

933.1

4.189.6

A No pruning

C

Mod. pruning

Ser. Pruning

D Nod. pruning 14A8

It Mod. pruning

14/25

SX

Em2

31

19

23

27

28

23

33

25

27

24

21

30 32

118

2890

128

33614

132

35145

118

2870

'14

Source of variation

Treatments

Replications

Irror

Total

14

3

12

19

69.7

30.4

271.1

3712

17.42

10.13

22.59

Sidearm Length (in.) in the Time and Severity of Pruning Trial en niggles, 3158.

Rep.I

Treat.

1 2 3 4 Total

A

Rep.I/

2 3 4

141

32 28 33 3 96

7' 4 2 13 3 22

0 0

8

0 10

0 7 0

10' 12 5 16 12

145

12 6 7 7

Total

1 2 3

10 19 37 22

15

32

18 13 5

19 14 9

4

8

9

Total

93

51

2

Rpar

3 4 T

O 39 30 23

114

16

114

9

28 33

23 9

Total 48 35 62 18 163 20 6 14 17

57

56 64 36

32 188

B 141

7'

0

114

2 12

5 11

7 6

28

29

0

14

6 0

9 8 6 8

10' 10

114

9 9

142

12 9 8 9

Total 15 39 18 27 99

21 21 20 17

10

31

38

79

8

9

0 11 0

7

5 12

5

17 23 3.7

39

17

15

71

50

37 la.

128

G itt 13 7 0

7' 9 7

5 12

0.

20 0 7 0 0

33

10' 12 8 7 11 38

0 11

4 4

31 12

7 9

7

19

39

8

0 13

10 10 11

9 14 13

27 24 37

0

10

21

39

46

D

Total

314

22 12 23 91

11 30 11 13

141

0 6 0 0 6 0

7' 5 7 16 6 34 8 17

10' 11 9 12 8 40 9 33

0 16

114

9 12

11 33

Total 16 22

28

114

80

65

42

146

17 30 36 39 122

E 41

7'

10'

0

17

15

0

8

0

7

O

O

9

0

25

39

0

6 7

0 20

0 20

11 32 36

10 11 17

21 59

Total

32 12 11

9 614

16 18 48

33 115

SI 1145 1,30131 91 497

314

9

0

9 28

12 9

18

48

50 21

143

70

0 0 0 0

7 9 6 5

8

114 u.

7

15 23 17 12

106

108

35

141

184

0

27

40

67

85 105 129 119 438 165 155 150 203 673 sx2

2363

1734

2187

869 835

1139

3567

1485 2835

2727

2196

5189

27 68 (fr 37 229

O 14

7

12 9 8

16 13 14 11

0

j

n

35

28 26 29 20

O

9

16

0 24 0

11 10

23 22

25 48 22

23

3.0

103

84

314 no

16 21 u

56

38 49 22 57 166

0 o 0 0

13 13 20 32

14 12 12 12

27 25 32 a

135 193 198 VA

3747

1863 3

© 32

1b,

2596

58

50

108

656

, erg 4965 5217

3858 1859

55147

1507

2601

14317

3357

41824

6879 5092

6171 11433

101309 5951 6075

391/ 9142 101530

&eta Mg

181

te*

D

E

603

3.62

3.72

4.21

4.08

00(mosaid, D4445:$ celeriac rio mothod

Pussies

!Mt

4.34

3.85

3.26

3.96

3.96

4.18

444

3.68

3.51.

4.22

Ba41

4.04

14.62

3.40

3.96

3.60

kiiit

1.6.59

16.63

1606

15.61

15.86

445

4.16

3.52

3.90

3.96

19.63

19.62

78.75

3.94

Treat.

Rep.

Error

Total

Analysis; of Variances

09194

06089

.34294

2.49577

4

3

32

19 cc

MS

0.2729

0.0203

0.1119

2.431

1.3.

M.3.

mid

8.145

oil 0.85

rug. a-acid 5.20

oil, 1.56

B.Q.

a-acid 9.43

oil 2.08

a-acid 6.17

oil 1.59

Hall a-acid 5.92

oil 1.49

103 -I a-acid 3.41

oil 0.92

a-acid 5.61

oil 1.40

108 -I **acid 8.56

oil 1.85

109-1 a-acid 6.93

oil 0.88

112 -1 a-acid 7.05

oil 1.59

123-I a-acid oil

124-1 a-acid oil

6,92

1.1?

6.82

1.32

127-1 a-acid 6.28

oil

128.1 a-acid 10,30 oil 1.44

129 -I a-acid 6.25

oil 1,42

1.32 -I a-acid oil

3.10

0.31

54 a-acid 2.85

oil 1.43

7.72

1.13

3.3?

0.33

MN&

.

3.77

0.90

5.18

0.95

8.14

1.58

6.43

0.83

9.96

1.20

8.84

042

$.58

1.21

NOS

8.39

1.05

8.33

1.21

6.21

1.54

144-I

3.72

0.38

ha%

1.20

8

4

6

7

1

Fugg.

ii.0.

Bull.

1031

107 -I

1081

109-1

2.67

2.78

2.59

2.43

3.36

3.64

3.39

3.48

4.28

4.67

4.28

4.39

5.56

5.94

5.74

5.38

5.78

.....,

7.59

7.44

7.147

7.88

7.84

7.65

7.40

4.43

4.64

4.61

4.28

5411

5.06

4.81

5.02

5.68

5.49

5006

5.45

6.24

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

0

3

6

10

.

..

.

._

..

2.68

2.42

1.92

2.21

3.85

3.95

3.63

3.69

3.67

3.76

3.34

3.57

3.78

3.67

3.50

3.12

7.10

5.87

5.28

5.20

5.80

4.45

3009

3.99

505%

5.35

5.43

5042

5.28

54.;2

5.85

34100

2.72

2.74

2.56

14.10

4.25

4.01

3,86

6.42

5.10

4.63

4.18

..

....

.00

3.43

3.76

3.11

3.40

5.09

5.37

5.06

4.79

5..73

5.85

5.76

5.42

5.64

5.57

5.33

8.88

8.44

8.86

8.32

..

....

....

...

..

7.32

7.67

6.86

6.99

3.06

2.65

2.72

2.45

3.92

4.21

3.73

608

3.75

3.94

3.60

3.65

4.94

3.83

3.71

3.52

6,10

4.68

4.29

4.12

4.50

5.09

4.47

4.41

.

5.00

5.10

14,86

4.83

7.68

7.87

7.14

6.78

5.34

5.39

5.25

5.39

8,04

7.93

7.80

7.52

7,60

7.76

7.26

7.20

3.29

341

3.00

2.88

4.28

4.10

3.88

3.73

6.30

5.40

4.99

4.72

5.35

4.77

4.23

4.88

3.47

3.68

3.26

3.39

3.97

4.3.6

3.85

3.87

9

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"C8

* Of

F.O.

nexem City, Washington

Dear Mr. Dwinal tour letter of March reviewed.

In accordance istratoris Mies has been advi tie* of the National Hop Research

C. Culbertson has been requirements, the Admin..

of the etleetivies and ergenisa.

Committee.

WS believe that the Committee will serve a very useful function and are pleased to approve the appointments of Mr. Stanley Brooks as representative for the U. S. Department of Agriculture and Dr. Chester

Horner as representatives for the state of 'Oregon.

For the Committee to accomplish the work of evaluating research results end preparing information for release to growers we agree that meetings should be held not lees than two and not more than three times each year.

Your organisation has taken, a mast progressive step in sponsoring the organization of a National Hop Research Committee.

The purpose of all State and Federal research on agricultural problems is to reduce production hazards, increase efficiency of crop production, and improve the quality and farm value of crops produced.

The job is not finished until research result3 are tads available to growers.

It is our hope that the new Committee will function effectively in helping to bridge the gap between research workers and growers.

Sincerely yours,

L. M. Fultz, Acting hief

Oilseed and Industrial

Crops Research Branch cc: M. W. Parker

J. O. Culbertson

S. N. Brooks

C. E. Horner

121?; ass

183

..COPTes

lanwert Norris Chief 14004001014 211011r04 and Asgard* Staff, Oral* itAAMInistraWre

ASS

L. S. Pelts, hating Obi41/01340041 awl

ImSnotrial Crepe Mosearch Bra m&

Organisation and purpose of National Sep Researeh Commit

At the roiliest of the Sop &ewors sf Anerisa, Ins., a national organism' hopgrowors, a National Sop Beseareh Committee has boon formed. The objeotives of the eommittee will hes (1) to provide for correlation of research aottvities and for exchange of rOseareh information among speeialists invpstigating hop production problems in the four hop-greying states; and (1) to provide for evaluation of research results and for dissemination of current findings among growers.

As new planned, the sommittee will be a continuing body until, t ted tor agreement among the sponsoring agency and members of the ttee.

Meetings of the National Hop Research Committees will be held not less than two nor wore than throe times per year.

For 1958 the menherthip of the Committee will be as follows*

Naas and Organisation Representing

S. N. Brooks Chairman of Committee

Research Agninemist, Crops lessareh

Division, ARS

B.S. Department of Agriculture

C. B. Skotland,. Spore

Plant Pathologist of the Committee*

Washington Agricultural Experiment Station

State of Washington

Hotter, AgenPathologitt)

Greps;HaCearchtDivisioni,ARS-anU

Oregon Agr icultural Eiperiment Station

State of Oregon

W. G. Golden Jr., Farm Advisor

California Agricultural Extension Service State of California

* R. R. Romanke* Plant Pathologist

Idaho Agricultural Experiment Station State of Idaho

* Omitted on original list through what is presumsd to be an oversight (Sip!).

ting of National Ho k Hotel, Yakima. Wishiagtes,

S. N. Brooks, Chai

USDA Oregon State

C. S. Horner, USDA and Oregon State

R4 R. Romanko, Idaho Agri. Experiment

W. G. Golden, Farm Advisor, Sacramento County, California

C. B. Skotland, Secretary, Washington Irrigation

Experiment Station, Presser of the conclusions reached,

1.

be one day before the Hop Growers

1 convention.

2.

Before this meeting an exchange of summary research reports.

Reports to be summarized as quickly as possible in the fall.

(This has been dons for the past season's work.)

During this meeting a combined state report will be drawn up based on the summary reports and will be submitted to the

Hop Growers of America, Inc.

3, Ross Dwinell, Executive Secretary of the Hop Growers of

America, Inc., is to contact each worker for information for his bulletin.

1.

Each member of the committee discussed his present program under investigation.

The committee =aborts agreed that it would be advisable for each research worker to observe work in progress at other stations, contingent on the availability of travel funds.

184

185

Dobta Romanke, Asst. Plant Pathologist, Idaho h ISpata.,Parsea, Ida*

B. N. Brooks, Research Agronomist, USDA and Oregon State College, Corvallis

C.

$. Horner, Plant Pathologist, USDA and Oregon State College, Corvallis

C. B. Nelsen, Agronomist, Washington State College, US, Prosser, Mesh.

G. B. Skotland, Asst. Plant Pathologist, Wash. State College, IRS, Prosser

Minutest

A short discassion was held on what should report of this committee to the "Hop Growers of suggestions were made:

1. This was to be a progress type

2* Reports should include description of the work and summary and interpretation of results.

Information to be included would be at the discretion of the individual researcher.

No recommendations to be included in this report since in most instances might have to be modified after further work.

List the publications for the current year from respective state.

Reports are to be handled as follows:

1.

Get report cleared through proper administrative charnaels

4 Send three copies (at least) to the secretary of the "National

Hop Research Committee."

The secretary is to combine am and forward them to the secretary of the "Hop Growers of America" as the annual report of the "National Hop Research Committee*" One copy is to be sent to chairman of NHRC, one other to be retained in secretarial files.

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