A FACT, A CONCEPT • The cell is THE fundamental unit of living organisms From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "WORLD BOOK illustrations by Charles CELL THEORY HISTORY • Robert Hooke observed cork and saw this: 2 IMAGINE • Some world made up of free moving materials – Under some conditions, linked materials may “survive” better than when free and independent 3 NECESSITIES TO SURVIVE AND PROLIFERATE • Separation from environment • Integrity • Energy source • Reproduction NECESSITIES TO SURVIVE AND PROLIFERATE • Separation from environment • Integrity • Energy source • Reproduction 2’ND LAW OF THERMODYNAMICS • All natural systems or processes move toward disorder or randomness (entropy) ENERGY SOURCES • Internal - storage problems OR • External - acquisition problems ENERGY-SOURCE PROBLEMS • Internal – Storage – Diffusion – Size effects From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert ENERGY-SOURCE PROBLEMS • External – Requires a permeable membrane but this can cause leakage – Energy needs to maintain integrity – Surface to volume problems – Waste From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert A SURFACE TO VOLUME PROBLEM • A 1 cm cube gives 6 1x1 cm surface = 6 cm2 and 1x1x1 cm volume = 1cm3 • 6:1 surface to volume SURFACE TO VOLUME CONTINUED • A 2 cm cube gives 6 2x2 cm surface = 24 cm2 and 2x2x2 cm volume = 8 cm3 • 24:8 surface to volume WHY IS SURFACE TO VOLUME IMPORTANT? • Cell content volume increase at a faster rate than the surface through which materials must be brought in to support contents From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert A FACT • Whenever you observe a cell with exaggerated surface area you can assume that it has a limited surface problem WHY IS SURFACE TO VOLUME IMPORTANT IN GENERAL? Limited surface area can constrain many reactions ENERGY-SOURCE PROBLEMS • External – Energy needs to maintain integrity – Surface to volume problems – Requires a permeable membrane but this can cause leakage – Waste From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert NECESSITIES TO SURVIVE AND PROLIFERATE • Separation from environment • Integrity • Energy source • Reproduction From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert REPRODUCTION • To increase representation in the population reproduction is essential "It's the first self-replicating cell on the planet that's parent is a computer,” Craig Venter REPRODUCTION • … “He has not created life, only mimicked it,” Dr. Baltimore said. THE CELL THEORY • All living organisms are composed of cells • Life-dependent chemical reactions occur within cells • Cells arise from other cells • Hereditary information is passed from mother to daughter cell From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "Chuck Brown, Photo Researchers COMPARISON PROKARYOTES Localized Small Always Nucleus Size Cell Wall EUKARYOTES Distinct Large Sometimes A FACT • 1 kilogram of fertile soil may contain more than 1 trillion individual prokaryotes! ENERGY USERS • Autotrophs - do NOT require external sources of organic materials. They obtain energy from light or inorganic materials • Heterotrophs - Require external sources of organic materials for energy and building block materials. From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book diagram by David Cunningham SOMETHING TO KEEP IN MIND • Form and function A FACT • All living cells are surrounded by a plasma membrane PLASMA MEMBRANE COMPONENTS • Lipid bilayer • Cholesterol (in animals) • Integral proteins • Peripheral proteins • Glycolipids IMPORTANT FACT • Phospholipids will spontaneously form bi-layers when immersed in water. MORE KITCHEN PHYSICS • WATER TENDS TO LOWER POTENTIAL IN 3 WAYS: – Gravity wise – Pressure wise – Concentration wise TWO EXTREMES Dilution Fresh Water Lake Dehydration Ocean A FACT • Diffusion requires random, independent movement of particles. A THOUGHT EXPERIMENT • Open a perfume bottle. If more perfume particles are inside the bottle at the outset there should be a net flow (to or from?) the bottle based on chance alone? A FACT • Water moves DOWN a concentration gradient, the steeper the faster. OSMOSIS • Diffusion of water across a semipermeable membrane Hypotonic Hypertonic HOW TO OVERCOME OSMOTIC TENDENCY • IN FRESH WATER - Use a water pump to maintain hypertonic condition • IN SEA WATER - (1) Use salt pump to maintain hypotonic condition or (2) maintain isotonic condition. 3 WAYS TO GET MATERIALS IN AND OUT OF CELLS • Osmosis • Carrier assisted - passive or active • Vesicular From The World Book (TM) Multimedia Encyclopedia (c) 1999 World Book, Inc., 525 W. Monroe, Chicago, IL 60661. All rights reserved. "World Book illustration by John F. Eggert ANOTHER IMPORTANT FACT • The rate of facilitated diffusion depends upon the number of protein facilitators and the rate of movement through them 2 TYPES OF VESICULAR TRANSPORT • Exocytosis - out of cell • Endocytosis - into cell 36