Barley Genetics as a Model for International Collaborative Research
by
Lauren A. Osborn
A PROJECT
submitted to
Oregon State University
University Honors College
Bioresource Research
and
International Degree
in partial fulfillment of
the requirements for the
degrees of
Honors Baccalaureate of Science in Biology
and Bioresource Research (Honors Associate)
Honors Baccalaureate of Arts in International Degree (Honors Associate)
Presented June 7, 2007
Commencement June 2007, for degrees awarded Spring term 2007
An Abstract of the Thesis of
Lauren A. Osborn for the degree of Bachelor of Science in Bioresource Resource
Research, Honors College and International Degree presented on June 7, 2007.
Title: Barley Genetics as a Model for International Collaborative Research
Chapter 1: Inheritance of Final Leaf Number in Dicktoo ×Oregon Wolfe Barley
Dominant and Dicktoo × Calicuchima barley F2 populations
Abstract approved: _____________________________________________
Patrick Hayes, Mentor
Date
Temperate grasses need an extended exposure to low temperature (i.e.
vernalization) in order to properly regulate the transition from vegetative to reproductive
growth. In barley and other cereals there are several methods to measure the sensitivity or
response to the vernalization. The three most commonly used phenotypes are the double
ridge stage of meristem development (Kirby and Appleyard 1987), heading date
(Wehrhahn and Allard 1965), and final leaf number (FLN) (Wang et al. 1995). Heading
date is the simplest to measure however maturity genes may influence the measurement.
While the double ridge method is the most accurate, it is extremely time consuming and
results in the loss of the plant. Therefore, final leaf number (FLN) was used to measure
the phenotype on two populations: Dicktoo × OWB-D and Dicktoo × Calicuchima.
Heading date was measured on the same populations (Szűcs et al. 2007) and these results
were compared using a statistical correlation to show if the maturity genes had a
significant effect on the data from the heading date results.
An Abstract of the Thesis of
Lauren A. Osborn for the degree of Bachelor of Science in Bioresource Resource
Research, Honors College and International Degree presented on June 7, 2007.
Title: Barley Genetics as a Model for International Collaborative Research
Chapter 2: International and Local Research Experience
Abstract approved: ____________________________________________________
Patrick Hayes, Mentor
Date
It is important to stay up to date with research especially when conducting
research yourself. In studying barley genetics, there are many researchers participating
around the world. I was able to participate in two research labs; one at Oregon State
University in the Crop and Soil Science Department in the lab of Dr. Hayes and one
during a study abroad at the University of Lleida in the Agricultural Department in the
lab of Dr. Romagosa. Both research experiences were examined, comparing Oregon State
University and the University of Lleida in the areas of history, current university status
and academics and research resources and atmospheres in the labs.
©Copyright by Lauren A. Osborn
June 7, 2007
All Rights Reserved
Barley Genetics as a Model for International Collaborative Research
by
Lauren A. Osborn
A PROJECT
submitted to
Oregon State University
University Honors College
Bioresource Research
and
International Degree
in partial fulfillment of
the requirements for the
degrees of
Honors Baccalaureate of Science in Biology
and Bioresource Research (Honors Associate)
Honors Baccalaureate of Arts in International Degree (Honors Associate)
Presented June 7, 2007
Commencement June 2007, for degrees awarded Spring term 2007
Honors Bachelor of Science and Honors Baccalaureate of Arts thesis of Lauren A.
Osborn presented on June 7, 2007
Approved:
Mentor, representing Crop and Soil Science
Date
Committee Member, Crop and Soil Science
Date
Committee Member, Horticulture
Date
Director, Department of Bioresource Research
Date
Director, International Degree Program
Date
Dean, University Honors College
Date
I understand that my thesis will become part of the permanent collection of Oregon Sate
University Libraries. My signature below authorizes release of my thesis to any reader
upon request.
Lauren A. Osborn
Date
ACKNOWLEDGEMENTS
First, I would like to thank my mentor, Dr. Patrick Hayes for the opportunity to
work in his lab, and his guidance over the past few years which has been invaluable.
Special thanks to Dr. Peter Szűcs who not only has served on my committee, but has
helped me tremendously with the statistical analysis of my project. I wish them the best
of luck in their future research. I would also like to thank Dr. Tony Chen and Dr. Kate
Fields who have also served on my committee as well as Dr. Ignacio Romagosa who was
my mentor during my study abroad time in Spain. Ann Corey and Tanya Filichkina have
been of great help and support to me as well as the rest of the Barley Group and deserve
many thanks.
On a more personal note, I want to thank my parents, Joel and Sherrie Osborn and
my family for their unending support to put me through college; I will never know how to
thank them enough. I would like to thank Darin Olson as well for his constant
encouragement through the most stressful times of my thesis writing. Also, my roommate
and great friend, Sarah Covelle, who has helped proofread my thesis and has encouraged
me throughout the process.
I would like to give a special thanks to Wanda Crannell, my Bioresource Research
advisor, for her incredible advice, support and work that she has done to help me meet all
requirements for my three degrees. My advisors in the Honors College and International
Degree also deserve thanks for their support. I would like to also thank Anita Azaranko
for introducing me to the Bioresource Research program and who has been an incredible
source of advice and support throughout my college career.
If there is anyone I’ve missed, I’d like to thank them also. If it had not been for all
the people involved I would have never been able to finish my thesis. Thank you!
TABLE OF CONTENTS
Page
Inheritance of Final Leaf Number in Dicktoo × Oregon Wolfe Barley Dominant
and Dicktoo × Calicuchima barley F2 populations
1
Introduction
1
Materials and Methods
6
Results
9
Discussion
13
Bibliography
14
International and Local Research Experience
16
Introduction
16
University of Lleida
18
History
18
University of Lleida Currently
19
Research Experience
20
Oregon State University
23
History
23
Oregon State University Currently
25
Research Experience
26
Comparison
27
Bibliography
30
LIST OF FIGURES
Figure
1. Frequency distribution for the FLN in the Dicktoo × OWB-D F2 population
Page
10
2. Frequency distribution for the FLN in the Dicktoo × Calicuchima F2 population 10
3. Mean FLN in the nine VRN-H genotype classes of the Dicktoo × OWB-D
F2 population
11
4. Mean FLN in the nine VRN-H genotype classes of the Dicktoo × Calicuchima
F2 population
12
LIST OF TABLES
Table
Page
Inheritance of Final Leaf Number in Dicktoo × Oregon Wolfe Barley Dominant
and Dicktoo × Calicuchima barley F2 populations
1. Genotypes (in the homozygous condition) of the winter, spring, and
facultative barley types
4
2. Vernalization alleles and phenotypes of three barley germplasm accessions
and expected F2 segregating patterns in their F2 progeny
7
3. FLN, growth habit and VRN-H allele combinations for parent and
F1 genotypes
9
International and Local Research Experience
1. Comparison results from the Web of Knowledge (1996–present)
27
Preface
Collaboration is crucial when advancing in scientific research. One person cannot
study all aspects of a system. That is why it is important for the scientific community to
communicate with each other and stay updated on research being conducted in other labs
around the world. In my research, I was able to incorporate research collaboration on an
international level. A term of study abroad at the University of Lleida in Spain, gave me
an opportunity to work on research in another country. In both research projects, Oregon
Wolfe Barley was used as a model for study and was the common ground on which the
two research experiences connected. My thesis consists of two chapters; one which
describes my research at Oregon State University working with the Barley Project in the
Crop and Soil Science Department, and my second chapter is a comparison between the
University of Lleida and Oregon State University.