2007 Extension Research Report Insect Management EFFICACY OF VENOM AND V-10170 APPLIED THROUGH DRIP IRRIGATION SYSTEM FOR MANAGEMENT OF SILVERLEAF IN SQUASH Alton N. Sparks, Jr., University of Georgia Cooperative Extension, Department of Entomology, Tifton, GA 31793, asparks@uga.edu of silverleaf were noted the day of application. These applications were applied through drip irrigation lines placed next to the plants (adjacent to the irrigation lines). An injection manifold was designed for this test to apply a single treatment to all four replications at one time. The manifold had a single injection port followed by a ‘mixing’ assembly (two 90 degree turns) followed by Tconnections leading to 4 solid injection lines (approximately 200 feet each). The injection lines were attached to 33 feet of drip tape. The drip tape was placed next to the plants of the specified plots for treatment. Once the tape was in place for all four plots of a treatment, the irrigation water was turned on and the system allowed to pressurize and run for approximately 5 minutes (and was checked for any leaks). The specified treatment (for all four plots) mixed in 3 liters of water was then injected into the running system. This required approximately 5 minutes to complete the injection. After injection of the treatment, the system was allowed to run for an additional 10 minutes to insure the full treatment was flushed from the irrigation lines and into the plots. The irrigation was then turned off and the lines were moved to the next set of plots and the process was repeated. After all treatments were applied, this system was set on the check plots and run for 20 minutes to insure equal moisture in the check treatment. Efficacy was evaluated by rating silverleaf symptom severity and yield in each plot. Plots were visually examined and rated on the amount and distribution of silverleaf symptoms as follows: 0 = no silverleaf Introduction The sweetpotato whitefly (a.k.a. silverleaf whitefly) can be a severe pest in Fall vegetable production in some areas of south Georgia. This pest is of particular concern in squash, where even low populations cause silverleaf symptoms. Neonicotinoid insecticides play a key role in managing this problem. Soil application of these products typically provides long residual control. This test was conducted to evaluate the efficacy and residual control of soil applied insecticides for management of silverleaf in squash. Materials and Methods A small plot trial was conducted at the University of Georgia Horticulture Farm in Tifton, Georgia. Squash, var. Destiny III, was direct seeded in single rows on six foot beds for this test on 7 Aug., 2006. Drip tape was immediately set on the rows to maintain adequate moisture for plant establishment and for irrigation during the season. After plant establishment, experimental plots measuring one row (assumed 36 inch for treatment calculations) by 33 feet were established in a randomized complete block design.. Treatments evaluated in the test were V10170 50 WD at 3 and 6 oz/ac, Venom 70SG at 7.5 oz/ac, and combination treatments of Venom at 7.5 oz/ac plus V-10170 at 3 and 6 oz/ac. These treatments were compared with Admire Pro 4.6F at 7.4 oz/ac and a non-treated control. All insecticides were applied through drip irrigation simulation. Insecticides applied through the drip system were applied on 15 Aug. Plants were well established at this time. Minimal signs 104 1 = light symptoms and spotty distribution within the plot 2 = light symptoms throughout the plot, or moderate symptoms and spotty 3 = moderate symptoms and even distribution 4 = heavy symptoms and spotty distribution 5 = heavy symptoms throughout the plot Yields were determined by hand harvesting each plot. On each harvest date, each plot was hand picked, with the entire plot picked. Fruit from each plot were counted and weighed. All data were analyzed with the PROC ANOVA procedure of PC-SAS. Where significant differences were detected (P<0.05), means were separated with LSD (P=0.05). performance of Admire in this tests is of concern. This may have resulted from the application technique (drip line on the soil surface and exposure to sunlight), but may also represent potential resistance development to the neonicotinoid insecticides. Even though Venom provided significant control in this test, it should be noted that silverleaf symptoms had increased in all treatments between 24 and 29 Aug. and the test was terminated after only two harvests because of overall poor condition of the plants. The 29 Aug. sample date was only 14 days after treatment, and both the Admire and Venom would be expected to provide at least 3 to 4 weeks of protection at the rates used. The lack of control may have also occurred because of the severe pest pressure; however, silverleaf is reported to result only from immature whiteflies. Silverleaf symptoms appearing at 14 days after treatment would suggest reproduction and survival of immatures within two weeks of treatment, which should not occur with either of these products under any level of pest pressure. Results and Discussion Pest pressure was extremely heavy in this test. The only treatments that statistically separated from the Check in the test were the three treatments that contained Venom (Table 1). Addition of V-10170 did not appear to improve performance of Venom. The relatively poor 105