Product datasheet
Anti-STAT5b antibody [EPR16671] (HRP) ab199570
2 Images
Overview
Product name
Anti-STAT5b antibody [EPR16671] (HRP)
Description
Rabbit monoclonal [EPR16671] to STAT5b (HRP)
Conjugation
HRP
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Recombinant fragment within Mouse STAT5b aa 600 to the C-terminus. The exact sequence is
proprietary.
Database link: P42232
Positive control
WB: K562, HeLa and NIH/3T3 whole cell lysates. IHC: Human spleen (paraffin embedded
sections)
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-STAT5b antibody [EPR16671] (ab178941)
Anti-STAT5b antibody (Alexa Fluor® 488) [EPR16671] (ab199767)
Anti-STAT5b antibody (Alexa Fluor® 647) [EPR16671] (ab199846)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Immunogen affinity purified
Clonality
Monoclonal
1
Clone number
EPR16671
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab199570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90
kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0
before commencing with IHC staining protocol.
Target
Function
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular
responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and
activates PRL-induced transcription.
Involvement in disease
Growth hormone insensitivity with immunodeficiency
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to
the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type
FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3.
Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK
leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by
PTPN2 negatively regulates prolactin signaling pathway.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Anti-STAT5b antibody [EPR16671] (HRP) images
2
All lanes : Anti-STAT5b antibody
[EPR16671] (HRP) (ab199570) at 1/5000
dilution
Lane 1 : K562 (Human erythromyeloblastoid
leukemia cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic
fibroblast cell line) Whole Cell Lysate
Western blot - Anti-STAT5b antibody [EPR16671]
Lysates/proteins at 10 µg per lane.
(HRP) (ab199570)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 90 kDa
Observed band size : 90 kDa
Exposure time : 1 minute
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 2% Bovine Serum Albumin before
being incubated with ab199570 overnight at
4°C. Antibody binding was visualised using
ECL development solution ab133406.
3
IHC image of STAT5b staining in a section of
formalin-fixed paraffin-embedded normal
human spleen, performed on a Leica
BONDTM. The section was pre-treated using
heat mediated antigen retrieval with
Tris/EDTA buffer (pH9, epitope retrieval
solution 2) for 20mins. The section was then
incubated with ab199570, 1/100 dilution, for
15 mins at room temperature. DAB was used
as the chromogen. The section was then
counterstained with haematoxylin and
Immunohistochemistry (Formalin/PFA-fixed
mounted with DPX. The inset negative control
paraffin-embedded sections) - Anti-STAT5b
image is taken from an identical assay
antibody [EPR16671] (HRP) (ab199570)
without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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