This is a supplement to your lab notes and your notes are the best guide for studying- note that not all terms and concepts may be listed below and that you should study only the labs that we reviewed this semester! Lab 8 Diffusion and Osmosis Define diffusion How does temperature affect the rate of diffusion? How does molecular weight affect trhe rate of diffusion? Describe selectively permeable as it relates to membranes. Define solute and concentration gradient, chemical potential. Compare hypertonic, isotonic, and hypotonic solutions Describe Osmosis. What is osmotic potential dependent upon? Define osmotic potential and osmotic pressure. Describe what causes plasmolysis in living plants. Describe the reaction of Elodea or Spirogyra when NaCl is added. Why did the solution in the osmometer move up the tube? Lab 9 Mitosis Know the events of Interphase, Mitosis and Cytokinesis. G1, S and G2, cell division DNA replication. Identify organelles of the cell that aid in cell division. What is a chromatid? What is a chromosome? What is a centromere? Homologous pairs, gene, allele, loci Phases of the cell cycle in the onion root tip and approximate % time spent in each. Identify models/slides in each phase of cell divison. *Lab 13 (used for reviewing Meiosis) Meiosis Handout (reference lab 13 in lab book) Know the events of Interphase, Meiosis and cytokinesis Describe each phase of meiosis I and II What is a Tetrad and crossover? When does this occur? Briefly describe how cells are able to increase diversity in the genetic makeup of gametes. Understand Independent assortment and segregation Homozygous, heterozygous, dominant, recessive, Linked and unlinked genes. 1 Drosophila Genetics Know the binomial name of the fruit fly- Drosophila melanogaster Know Life stage of fruit fly and how long they stay in each stage and how long they live. What is the importance of Virgin flies? How to identify the sex of fruit fly General Procedure for sexing them Be able to calculate F1 generation, F2 generations for both dominant and recessive genes. What type of mutants did we work with and which were recessive/dominant traits. What is the phenotype for wild type. Be able to recreate F1 cross and F2 cross and determine genotypes and phenotypes of each mutation- Be able to use the correct nomenclature for each phenotype i.e. wild vs. mutant. Lab 15- Human genetics Traits and karyotyping handout Be able to Identify the karyotypes presented in lab and their general phenotypes. Define terms presented in handout and be able to use them appropriately Genetic I- Monohybrid cross Be able to workout any problem in the worksheets Define terms presented in handout and be able to use them appropriately Genetic II- Dihybrid cross Be able to workout any problem in the worksheets Define terms, symbols presented in handout and be able to use them appropriately Genetic III - Pedigree Be able to workout any problem in the worksheets Define terms, legends, Mode of inheritance presented in handout and be able to use them appropriately 2 *Diversity Lab 22 and 23 Know the various Kingdoms and Domains (specific examples seen in lab) Be able to ID slides of the various kingdoms Describe morphology of bacteria and be able to ID What type of organism is penicillin Know the various types of Fungi and examples of each Ascomycota, basiodiomycota and zygomycota (be able to draw or ID general structure Describe a protista and modes of locomotion. What is symbiosis? Mutualism? Commensalism? Know Examples seen in lab Describe characteristics of Lichen and be able to ID Fructiose, Foliose, and Crustose What is nitrogen fixation and describe the biochemical pathway Describe Rhizobium and its symbiotic relationship with some plants. Where do you find Rhizobium? Know the various types of Algae Know the structure for plant and animal cells Diversity of Plants 24 Be able to distinguish between tracheophytes from non tracheophytes. What is a gymnosperm? What is an angiosperm? Archegonia vs. anther Microspores vs. megaspores Identify structures of a flower Identify male vs female plant structures Describe evolution of water to land (i.e. vascularization and mechanisms of fertilization. *Population Genetics CalculationsBe able to write out the Hardy-Weinberg equation and how to solve problems. What is the frequency of Homozygous recessive, Heterozygous dominant, and heterozygous specimens if given a example population. Define gene pool, population, allele, genotype, phenotype, allelic frequency, genotypic frequency What are some of the conditions that must exist in order for this HardyWeinberg equation to exist? 3