Product datasheet
Anti-Nurr1 antibody ab93332
2 Abreviews 3 Images
Overview
Product name
Anti-Nurr1 antibody
Description
Rabbit polyclonal to Nurr1
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide comprising an internal sequence of Human Nurr1 protein.
Positive control
Human hippocampus lysate; adult Rat hippocampus tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab93332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
IHC-P
Application notes
IHC-P: 1/100 - 1/200.
WB: 1/1000 - 1/2000. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Function
Transcriptional regulator which is important for the differentiation and maintenance of mesodiencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a
set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of
mdDA neurons.
Tissue specificity
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in
brain tissue.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Developmental stage
Rapidly and only very transiently expressed after cell activation, during the G0-G1 transition of
the cell cycle.
Cellular localization
Nucleus.
Anti-Nurr1 antibody images
All lanes : Anti-Nurr1 antibody (ab93332) at
1/1000 dilution
Lane 1 : MW marker
Lane 2 : Human hippocampus tissue lysate
at 20 µg
Lane 3 : Human hippocampus tissue lysate
at 20 µg with immunizing peptide
Lane 4 : Human hippocampus tissue lysate
at 20 µg
Western blot - Nurr1 antibody (ab93332)
Secondary
AP-conjugated secondary antibody at 1/5000
dilution
Predicted band size : 67 kDa
ab93332 at 1/100 staining Nurr1 in 20 µm
thick sections of formalin-fixed adult Rat
hippocampus tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Nurr1 antibody
(ab93332)
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ab93332 staining Nurr1 in human brain tissue
by Immunohistochemistry (Formalin/PFAfixed paraffin-embedded sections).
Tissue was fixed in formaldehyde and an
enzymatic antigen retrieval step was
performed using Proteinase K. Samples were
then incubated with ab93332 at a 1/250
dilution for 20 minutes at 25°C. The
secondary used was an undiluted HRP
Immunohistochemistry (Formalin/PFA-fixed
conjugated goat anti-mouse/ rabbit polyclonal.
paraffin-embedded sections) - Anti-Nurr1 antibody
(ab93332)
This image is courtesy of an anonymous Abreview.
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