Overview
Product name
Description
Conjugation
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (Alexa Fluor® 488)
Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker (Alexa Fluor® 488)
Alexa Fluor® 488. Ex: 495nm, Em: 519nm
ICC/IF
Reacts with: Human
Does not react with: Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caveolin-1 aa 150 to the C-terminus.
ICC/IF: HeLa cells.
.
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Caveolin-1 antibody [E249] - Caveolae Marker ( ab32577 )
Anti-Caveolin-1 antibody (Alexa Fluor 647) [E249] - Caveolae Marker ( ab193892 )
Anti-Caveolin-1 antibody (HRP) [E249] - Caveolae Marker ( ab193893 ) property license from Life Technologies Corporation. As this product contains the Alexa Fluor
® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer
(whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor
® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing
5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Clone number
Isotype
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Immunogen affinity purified
Monoclonal
E249
IgG
Applications
Our Abpromise guarantee covers the use of ab185043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
ICC/IF 1/50.
Target
Function
Tissue specificity
Involvement in disease
Sequence similarities
Post-translational modifications
Cellular localization
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to
DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1mediated signaling through the Wnt pathway.
Expressed in muscle and lung, less so in liver, brain and kidney.
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3)
[MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3).
Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Belongs to the caveolin family.
The initiator methionine for isoform Beta is removed during or just after translation. The new Nterminal amino acid is then N-acetylated.
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (Alexa Fluor® 488) images
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ab185043 staining Caveolin-1 in HeLa cells.
The cells were fixed with 100% methanol
(5min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1%
BSA/10% normal goat serum/0.3M glycine in
0.1%PBS-Tween for 1h. The cells were then incubated with ab185043 at a working dilution of 1 in 50 (shown in green) and ab7291
(Mouse monoclonal [DM1A] to alpha Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h
Immunocytochemistry/ Immunofluorescence -
Anti-Caveolin-1 antibody [E249] - Caveolae Marker
(Alexa Fluor® 488) (ab185043) secondary ( ab150120 ) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with
DAPI.
Image was taken with a confocal microscope
(Leica-Microsystems, TCS SP8).
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