Product datasheet
Anti-EPO antibody ab66071
2 Images
Overview
Product name
Anti-EPO antibody
Description
Mouse monoclonal to EPO
Tested applications
ELISA, WB, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Recombinant full length protein (Human) with tag.
Positive control
EPO transfected 293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab66071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
Use at an assay dependent dilution.
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 28 kDa
(predicted molecular weight: 21 kDa).
ICC/IF
Use a concentration of 10 µg/ml.
Target
1
Relevance
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated
cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma
and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin
synthesis. This protein also has neuroprotective activity against a variety of potential brain
injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of
adult mammals and by liver of fetal or neonatal mammals.
Cellular localization
Secreted
Anti-EPO antibody images
All lanes : Anti-EPO antibody (ab66071) at 1
µg/ml
Lane 1 : EPO transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate.
Lysates/proteins at 50 µg per lane.
Western blot - EPO antibody (ab66071)
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500
dilution
Predicted band size : 21 kDa
Observed band size : 28 kDa
ICC/IF image of ab66071 stained HepG2
cells. The cells were 4% formaldehyde (10
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab66071, 10µg/ml)
overnight at +4°C. The secondary antibody
(green) was ab96899 Dylight 488 goat antiImmunocytochemistry/ Immunofluorescence Anti-EPO antibody (ab66071)
rabbit IgG (H+L) used at a 1/250 dilution for
1h. Alexa Fluor® 594 WGA was used to label
plasma membranes (red) at a 1/200 dilution
for 1h. DAPI was used to stain the cell nuclei
(blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
2
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3