Product datasheet
Anti-Apolipoprotein B antibody [EPR2914] ab139401
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Overview
Product name
Anti-Apolipoprotein B antibody [EPR2914]
Description
Rabbit monoclonal [EPR2914] to Apolipoprotein B
Tested applications
Flow Cyt, IP, WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Positive control
Human plasma and Human serum lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20ºC.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR2914
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab139401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
Flow Cyt
Notes
1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
IP
Use at an assay dependent concentration.
WB
1/10000 - 1/50000. Predicted molecular weight: 515 kDa.Can be blocked with
Human Apolipoprotein B peptide (ab199783).
Application notes
Is unsuitable for ICC/IF or IHC-P.
Target
Function
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100)
and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and
internalization of LDL particles by the apoB/E receptor.
Involvement in disease
Hypobetalipoproteinemia, familial, 1
Familial ligand-defective apolipoprotein B-100
Defects in APOB associated with defects in other genes (polygenic) can contribute to
hypocholesterolemia.
Sequence similarities
Contains 1 vitellogenin domain.
Post-translational
modifications
Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the
lipoprotein particle.
Cellular localization
Cytoplasm. Secreted.
Anti-Apolipoprotein B antibody [EPR2914] images
Overlay histogram showing HepG2 cells
stained with ab139401 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
(ab139401, 1/1000 dilution) for 30 min at
Flow Cytometry - Anti-Apolipoprotein B antibody
22°C. The secondary antibody used was
[EPR2914] (ab139401)
Alexa Fluor® 488 goat anti-rabbit IgG (H+L)
(ab150077) at 1/2000 dilution for 30 min at
22°C. Isotype control antibody (black line)
was rabbit IgG (monoclonal) (0.1μg/1x106
cells) used under the same conditions.
Unlabelled sample (blue line) was also used
as a control. Acquisition of >5,000 events
were collected using a 20mW Argon ion laser
(488nm) and 525/30 bandpass filter.
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All lanes : Anti-Apolipoprotein B antibody
[EPR2914] (ab139401) at 1/10000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000
Western blot - Anti-Apolipoprotein B antibody
dilution
[EPR2914] (ab139401)
Predicted band size : 515 kDa
Western blot analysis on Immunoprecipitation
pellet from human serum lysate using antiApolipoprotein B RabMAb (ab139401).
Immunoprecipitation - Anti-Apolipoprotein B
antibody [EPR2914] (ab139401)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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