Product datasheet
Anti-TRA2B antibody ab31353
1 Abreviews 11 References 5 Images
Overview
Product name
Anti-TRA2B antibody
Description
Rabbit polyclonal to TRA2B
Tested applications
CLIP, IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TRA2B.
Read Abcam's proprietary immunogen policy (Peptide available as ab32689.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Hela, Jurkat, A431,
HEK293 This antibody gave a positive signal in the following methanol fixed cell line: HeLa.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab31353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
CLIP
Use at an assay dependent concentration. PubMed: 22194695
IHC-P
1/300. Perform heat mediated antigen retrieval before commencing with IHC
staining protocol.
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Application
Abreviews
WB
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa
(predicted molecular weight: 33 kDa). Although the predicted molecular mass of
TRA2B is ~33 kDa, the available literature describes TRA2B running at ~40 kDa
in SDS-PAGE
ICC/IF
Use a concentration of 1 µg/ml.
Target
Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Tissue specificity
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta
and brain. Lowest expression in kidney and liver.
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications
Phosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric.
Cellular localization
Nucleus.
Anti-TRA2B antibody images
ICC/IF image of ab31353 stained HeLa cells.
The cells were 100% methanol fixed (5 min)
then permeabilised using 0.1% PBS-Triton
and then incubated in 1%BSA / 10% normal
goat serum / 0.3M glycine in 0.1% PBSTween for 1h to further permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab31353 at 5µg/ml
overnight at +4°C. The secondary antibody
(pseudo-colored green) was Alexa Fluor®
Immunocytochemistry/ Immunofluorescence -
488 goat anti- rabbit (ab150081) IgG (H+L)
Anti-TRA2B antibody (ab31353)
preadsorbed, used at a 1/1000 dilution for 1h.
Alexa Fluor® 594 WGA was used to label
plasma membranes (pseudo-colored red) at
a 1/200 dilution for 1h at room temperature.
DAPI was used to stain the cell nuclei
(pseudo-colored blue) at a concentration of
1.43µM for 1hour at room temperature.
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Image courtesy of Human Protein Atlas .
ab31353 staining TRA2B in human pancreas,
showing a distinct and strong nuclear staining
pattern in both exocrine pancreas and islet
cells. Paraffin embedded human pancreas
tissue was incubated with ab31353 (1/300
dilution) for 30 mins at room temperature.
Antigen retrieval was performed by heat
induction in citrate buffer pH 6.
ab31353 was tested in a tissue microarray
Immunohistochemistry (Formalin/PFA-fixed
(TMA) containing a wide range of normal and
paraffin-embedded sections) - TRA2B antibody
cancer tissues. Further results for this
(ab31353)
antibody can be found at
www.proteinatlas.org
All lanes : Anti-TRA2B antibody (ab31353)
at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblastlike cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney
cell line) Whole Cell Lysate
Western blot - TRA2B antibody (ab31353)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 40 kDa
Exposure time : 5 minutesAlthough the
predicted molecular mass of TRA2B is ~33
kDa, the available literature describes TRA2B
running at ~40 kDa in SDS-PAGE.
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Anti-TRA2B antibody (ab31353) at 1/100
dilution + NSC34 mouse cell lysate at 20 µg
Secondary
IRDYE® 800CW Donkey anti-Rabbit IgG at
1/3000 dilution
developed using the ECL technique
Western blot - Anti-TRA2B antibody (ab31353)
Performed under reducing conditions.
This image is courtesy of an anonymous Abreview.
Predicted band size : 33 kDa
Observed band size : 42 kDa
Exposure time : 1 minute
This image is courtesy of an anonymous
Abreview.
Immunohistochemical analysis of formalinfixed, paraffin-embedded mouse testis tissue,
staining TRA2B with ab31353. Staining was
detected using DAB.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-TRA2B
antibody (ab31353)
Image from Grellscheid S et al., PLoS Genet 7(12):
e1002390. Fig 1.; doi:
10.1371/journal.pgen.1002390. Epub 2011 Dec 15.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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