Product datasheet
Anti-KDM5B / PLU1 / Jarid1B antibody ab56759
3 Abreviews 6 Images
Overview
Product name
Anti-KDM5B / PLU1 / Jarid1B antibody
Description
Mouse monoclonal to KDM5B / PLU1 / Jarid1B
Tested applications
WB, IHC-P, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment: AKRMRAEAMN IKIEPEETTE ARTHNLRRRM GCPTPKCENE
KEMKSSIKQE PIERKDYIVE NEKEKPKSRS KKATNAVDLY VCLLCGSGN, corresponding to
amino acids 231-320 of Human PLU1/ Jarid1B
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab56759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 190 kDa.
IHC-P
Use a concentration of 1 µg/ml.
ICC/IF
Use a concentration of 1 µg/ml.
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Application
Abreviews
Notes
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as
Flow Cyt
an isotype control with this antibody.
Target
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in
histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates
trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional
corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by
repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a
tumor suppressor for melanoma.
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and
glioblastoma. Up-regulated in breast cancer (at protein level).
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers.
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity.
Cellular localization
Nucleus.
Anti-KDM5B / PLU1 / Jarid1B antibody images
PLU1 / Jarid1B antibody (ab56759) used in
immunohistochemistry at 1ug/ml on formalin
fixed and paraffin embedded human colon.
IHC-P - PLU1 / Jarid1B antibody (ab56759)
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Predicted band size : 190 kDa
Western blot - KDM5B / PLU1 / Jarid1B antibody
(ab56759)
All lanes : Anti-KDM5B / PLU1 / Jarid1B
antibody (ab56759) at 1/500 dilution
Lane 1 : Human Pancreatic Cancer whole
cell lysate (Line AsPc1)
Lane 2 : Human Pancreatic Cancer whole
cell lysate (line Panc1)
Lane 3 : Human Pancreatic Cancer whole
Western blot - KDM5B / PLU1 / Jarid1B antibody
cell lysate (Line CaPan1)
(ab56759)
Lysates/proteins at 35 µg per lane.
Secondary
An HRP-conjugated Sheep anti-mouse
polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under non-reducing conditions.
Predicted band size : 190 kDa
Observed band size : 190 kDa
Exposure time : 15 minutes
This image is courtesy of an Abreview
submitted by Pawel Mazur
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ab56759 staining KDM5B/PLU1/Jarid1B in
Human pancreatic cancer tissue sections by
Immunohistochemistry (IHC-P - formaldehydefixed, paraffin-embedded sections). Tissue
was fixed with paraformaldehyde,
permeabilized with PBS + Triton 0.025% and
blocked with 10% serum for 1 hour at room
temperature; antigen retrieval was by heat
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - KDM5B / PLU1 /
Jarid1B antibody (ab56759)
This image is courtesy of an anonymous Abreview
mediation in citrate buffer. Samples were
incubated with primary antibody (1/100 in
PBS) for 8 hours at 4°C. A Biotin-conjugated
Goat anti-mouse IgG polyclonal (1/1000) was
used as the secondary antibody.
ICC/IF image of ab56759 stained HeLa cells.
The cells were 4% PFA fixed (10 min) and
then incubated in 1%BSA / 10% normal goat
serum / 0.3M glycine in 0.1% PBS-Tween for
1h to permeabilise the cells and block nonspecific protein-protein interactions. The cells
were then incubated with the antibody
(ab26759, 1µg/ml) overnight at +4°C. The
secondary antibody (green) was Alexa Fluor®
Immunocytochemistry/ ImmunofluorescenceKDM5B / PLU1 / Jarid1B antibody(ab56759)
488 goat anti-mouse IgG (H+L) used at a
1/1000 dilution for 1h. Alexa Fluor® 594 WGA
was used to label plasma membranes (red) at
a 1/200 dilution for 1h. DAPI was used to
stain the cell nuclei (blue) at a concentration of
1.43µM.
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Overlay histogram showing MCF7 cells
stained with ab56759 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-KDM5B / PLU1 / Jarid1B
antibody(ab56759)
(ab56759, 1µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG2a [ICIGG2A] (ab91361,
1µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed.
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