Product datasheet
Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (FITC)
ab179898
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Overview
Product name
Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (FITC)
Description
Mouse monoclonal [5MC-CD] to 5-methylcytosine (5-mC) (FITC)
Conjugation
FITC. Ex: 493nm, Em: 528nm
Tested applications
Southern Blot, ICC/IF
Species reactivity
Reacts with: a wide range of other species
Immunogen
Chemical/ Small Molecule corresponding to 5-methylcytosine (5-mC) conjugated to Bovine
Serum Albumin (BSA).
Positive control
Mouse embryonic stem cells; Chlamidomonas me-1 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark.
Storage buffer
Constituents: 50% Glycerol, 50% PBS
Purity
Purified IgM
Purification notes
ab179898 is filter-sterilised.
Clonality
Monoclonal
Clone number
5MC-CD
Isotype
IgM
Applications
Our Abpromise guarantee covers the use of ab179898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Southern Blot
Abreviews
Notes
1/1000.
Immuno-blotting detection of DNA with 5-methylcytosine on nitrocellulose.
ICC/IF
1/50 - 1/100.
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Target
Form
The native context of double-stranded DNA may obstruct antibody binding to 5-methylcytosine.
For successful detection of 5-methylcytosine, we recommend that the DNA is denatured to make
the nucleotides accessible for the antibody. Denaturing methods vary depending on each
application.
Anti-5-methylcytosine (5-mC) antibody [5MC-CD] (FITC) images
Immunofluorescent analysis
of Chlamidomonas me-1 cells, labeling 5Methyl Cytosine with ab179898 at 1/50
dilution, followed by a FITC-conjugated
secondary antibody (middle panel). Left:
DAPI-stained cells. Right: Merged
image. Chloroplast DNA is exclusively
methylated in gamete cells (bottom row), as
opposed to vegetative cells (top row).
Immunocytochemistry/ Immunofluorescence Anti-5-methylcytosine (5-mC) [5MC-CD] antibody
(FITC) (ab179898)
Immunofluorescent analysis of mouse
embryonic stem cells labeling 5-Methyl
Cytosine with ab179898 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence Anti-5-methylcytosine (5-mC) [5MC-CD] antibody
(FITC) (ab179898)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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