DEPARTMENT OF PHARMACY UNIVERSITY OF MALTA Pharmaceutics IV PHR4308 Practicals Handbook Practical 1: Buffering Pharmaceutical Preparations Practical 2: Extemporaneous Preparations Practical 3: Particle Size Distribution Pharmaceutics Co-ordinator: Prof Anthony Serracino-Inglott Compiled by Marie Clare Zammit Updated by Nicolette Bartolo January 2012 PHR 4308 Practical 1 BUFFERING PHARMACEUTICAL PREPARATIONS Name: Group: Date: AIM Investigation of the buffering capacity of systems of interest to the pharmaceutical industry APPARATUS Beakers 4 x 100ml Burette 1 x 50ml Electronic balance Magnetic stirrer Measuring cylinder 1 x 50ml pH meter MATERIALS Boric acid Citric acid Phosphoric acid 2g/100ml Potassium Dihydrogen Phosphate Sodium Hydroxide 0.1M Sodium Hydroxide 0.5M WARNING: The pH meter electrode is very fragile. Handle with care. Always rinse well with water after using it. Leave in fresh distilled water when ready. METHOD i. Fill two burettes, one with the 0.1M and the other with the 0.5M sodium hydroxide solution. ii. Accurately weight about 0.155g of boric acid. iii. Dissolve the boric acid in 50ml water using a magnetic stirrer. iv. Place the pH meter electrode in the solution making sure that the stirrer does not hit the electrode bulb (lower end of electrode). v. When the pH is steady take the reading. vi. Add 1ml of 0.1M sodium hydroxide solution, from the burette, to the boric acid solution while stirring and keeping the pH meter electrode in the solution. When adding the sodium hydroxide solution, make sure that it goes directly into the solution. vii. Take the reading of the pH when it is steady. viii. Keep repeating step v and vi until the pH is above 11. ix. Accurately weight about 2.01g of citric acid and repeat steps ii to vii using 0.5M sodium hydroxide. Continue until the pH is above 12. ASI/NB/MCZ/MZA 2012 2 PHR 4308 x. Place 50ml of phosphoric acid in a beaker and use a magnetic stirrer to mix the solution. xi. Place the pH meter electrode in the solution making sure that the stirrer does not hit the electrode bulb. xii. When the pH is steady take the reading. xiii. Keeping the electrode in the solution and the solution stirred, add 1.0ml of 0.5M sodium hydroxide solution from a burette making sure that the sodium hydroxide goes directly in the solution. xiv. When the pH is steady take the reading. xv. Keep repeating steps xii and xiii until the pH is above 11. xvi. Accurately weight about 0.354g of potassium dihydrogen phosphate. xvii. Dissolve in 50ml water using the magnetic stirrer. xviii. Place the pH meter electrode in the solution making sure that the stirrer does not hit the electrode bulb. xix. When the pH is steady take the reading. xx. Keeping the electrode in the solution and the solution stirred, add 1.0ml of 0.1M sodium hydroxide solution from a burette making sure that the sodium hydroxide goes directly in the solution. xxi. When the pH is steady, take the reading. xxii. Keeping repeating steps xix and xx until the pH is above 11.5. QUESTIONS 1. Calculate the molarity of each of the solutions used. Calculations Solution Molarity Boric acid Citric acid Phosphoric acid 2g/100ml Potassium Dihydrogen Phosphate ASI/NB/MCZ/MZA 2012 3 PHR 4308 2. Plot a graph of pH against volume of sodium hydroxide added for each solution. Comment on the graphs obtained. ASI/NB/MCZ/MZA 2012 4 PHR 4308 ASI/NB/MCZ/MZA 2012 5 PHR 4308 ASI/NB/MCZ/MZA 2012 6 PHR 4308 ASI/NB/MCZ/MZA 2012 7 PHR 4308 3. What inferences can be made from the graphs obtained in 2? _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ 4. Using a mixture of the buffers studied, how would it be possible to have a buffer capable of buffering between pH 2 and pH 9? _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ _________________________________________________________________________ ASI/NB/MCZ/MZA 2012 8 PHR 4308 REFERENCES 1. Aulton ME, ed. Pharmaceutics: The science of dosage form design. 2nd ed. New York: Churchill Livingstone; 2002. 2. European Pharmacopoeia. 7th ed. Germany: Druckerei CH Beck, Volume1 2010:746-7. 3. Remington: The science and practice of pharmacy. 21st ed. UK: Pharmaceutical press; 2011. 4. Sinko PJ. Martin’s physical pharmacy and pharmaceutical sciences. 6th ed. US: Lippincott Williams & Wilkins; 2011. Demonstrator Name ASI/NB/MCZ/MZA 2012 Signature 9 PHR 4308 Practical 2 EXTEMPORANEOUS PREPARATIONS Name: Group: Date: AIM To make an extemporaneous preparation of an ointment and suspension APPARATUS Electronic balance Glass plate Wooden spatula Plastic container Mortar and pestle Glass funnel Glass bottle Labels Gloves MATERIALS Petroleum jelly Salicylic acid Tablet X 100mg Sodium benzoate METHOD Ointment preparation i. Calculate the amount of petroleum jelly and salicylic acid required to produce 10g of ointment containing 1% salicylic acid. ii. Clean and dry well the glass plate. iii. Place the glass plate on the balance and press Tare. iv. Weigh accurately the amount of petroleum jelly required, as calculated. v. Remove the glass plate from the balance and put the plastic container instead and press tare. vi. Weigh accurately the amount of salicylic acid required, as calculated. vii. Put the salicylic acid on the ointment and mix them by moving the spatula in the figure of 8 repeatedly. viii. Collect the prepared ointment using the wooden spatula and place it in the plastic container used to weigh the salicylic acid. ix. Mix the ointment again in the plastic container to ensure that any traces of salicylic acid powder previously left in the container are amalgamated well into the ointment. ASI/NB/MCZ/MZA 2012 10 PHR 4308 x. Label the ointment produced. xi. Repeat steps ii to ix to prepare 10g of ointment containing petroleum jelly and salicylic acid at a ratio of 11:0.25 respectively. Calculations: Amount of salicylic acid required for preparation 1: ________g Amount of salicylic acid required for preparation 2: ________g Suspension preparation A patient came to the pharmacy with the following prescription: Joe Borg Borg Street Msida Age: 50 Rx 1/1/2012 Compound a 10mg/ml of suspension X pt to take 50mg bd x5 days Dr Borg Reg No 0000 Clinic Street Msida i. ii. iii. iv. v. vi. vii. viii. ix. x. Provided that the dosage strength of the tablets available containing substance X is 100mg, calculate the amount of tablets X and volume of water required to produce a 10mg/ml suspension. Calculate the weight of sodium benzoate required (0.1% w/v) for the suspension, to act as a preservative. Mix the sodium benzoate with the calculated volume of water (referred to as vehicle). Pulverise the tablets using a mortar and pestle. Add one third of the vehicle to the pulverised tablets and triturate the powder until a uniform suspension is achieved. Transfer the suspension produced into the glass bottle using a funnel. Use one third of the vehicle to rinse the mortar and pestle by a triturating motion and transfer the vehicle to the bottle. Use the remaining volume of vehicle to rinse the funnel. Shake the produced suspension well. Label the bottle containing the produced suspension. ASI/NB/MCZ/MZA 2012 11 PHR 4308 Calculations: Quantity of tablets X required: ________ Volume of suspension required: ________ml Weight of sodium benzoate required: ________g Questions 1. What information should be included on the label of extemporaneous preparations? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ 2. What is the shelf-life of the extemporaneous preparations produced? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ 3. Why is the ointment slab used made of glass? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ 4. What volume of suspension X should be taken daily? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ ASI/NB/MCZ/MZA 2012 12 PHR 4308 5. What type of tablets should not be used for the preparation of suspension? Why? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ 6. Describe the ideal containers for the storage of extemporaneous ointments and syrups. ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ _____________________________________________________________________ REFERENCES 1. Roche®. Tamiflu® (oseltamivir) – Product information. [Online]. [cited 2012 Feb 1]. Available from: URL: http://www.roche-australia.com/fmfiles/re7229005/downloads/antivirals/tamiflu-pi.pdf Demonstrator Name ASI/NB/MCZ/MZA 2012 Signature 13 PHR 4308 Practical 3 Particle Size Distribution Name: Group: Date: Powders generally consist of particles with irregular shapes and non-uniform sizes. This means that size distributions are often complex. Particle size is therefore described by determining the number of particles in successive size ranges. The distribution is often represented by a histogram in which the width of the bar represents the size range and the height represents the frequency of occurrence in each range. A smooth curve can then be drawn through the midpoints of the tops of the bars. A particle size distribution curve is obtained in this way. In order to obtain a distribution curve, the powder is separated into fractions depending on particle size. The most frequently used method to separate powders is sieving. Basically, this technique consists of placing a nest of sieves of different aperture on top of one another with the finest aperture sieve being placed at the bottom and subsequent sieves being placed in ascending degrees of coarseness. The sieves are then shaken mechanically or electromagnetically. The sieve motion applied can be oriented horizontally which tends to loosen the packing of the particles in contact with the sieve surface, or vertically which tends to agitate and mix the particles. Method i. Accurately weigh each sieve to be used, to the nearest 0.1g. ii. Set up the nest of sieves on the mechanical shaker in an ascending order i.e. the finest sieve is at the bottom and the coarsest sieve is on top. iii. Accurately weigh out approximately 25g, 50g and 100g of the powder. iv. Place the first sample carefully on the topmost sieve and close the cover. v. Shake the powder through the sieves at 50% amplitude for 5 minutes. Then clean the sieves and repeat for each sample size so as to determine what sample size should be used. vi. Accurately weigh the approximate sample size to be tested. Clean and accurately weigh once again each sieve. vii. Place the sample on the top most sieve, close the lid and vibrate at 50% amplitude until the end-point is reached. This is achieved when the weight held on each one of the sieves does not change by more than 5% or 0.1g (or 20% if the weight on that particular sieve is less than 5% of the test sample size). viii. If any sieve is found to hold more than 50% of the test sample, then the test is to be repeated with the inclusion of an additional sieve of intermediate coarseness between this sieve and the next coarser one. You should repeat the test if more than 5% of your sample is lost. ASI/NB/MCZ/MZA 2012 14 PHR 4308 ix. Your report must include the total sieving time, the methodology used (Dry Sieving Method) and the set values for all variable parameters. Questions 1. Draw a histogram of your particle size distribution and a particle size distribution curve. Comment on the shape of this curve. Comment on the shape of this curve. ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ASI/NB/MCZ/MZA 2012 15 PHR 4308 2. What is the finest particle size you would normally use this method for? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 3. What problems can be encountered if the particle size is finer than this? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 4. What is the commonest difficulty encountered when testing particle size by this method? How can this problem be overcome? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 5. What settings can be varied in this test? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ 6. How should sieves be cleaned? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ASI/NB/MCZ/MZA 2012 16 PHR 4308 7. If electrostatic charge is affecting your analysis, what corrective action should you take? ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ ______________________________________________________________________ Demonstrator Name ASI/NB/MCZ/MZA 2012 Signature 17 PHR 4308 ASI/NB/MCZ/MZA 2012 18