The Georgia Agricultural Experiment Stations College of Agricultural and Environmental Sciences The University of Georgia Research Report Number 717 October 2008 Insect Screening Results Evaluation of Insect Resistance in Commercial Corn Hybrids Xinzhi Ni, Jeffrey P. Wilson, Brian T. Scully, and G. David Buntin In 2008, 65 commercial hybrids were examined for insect resistance at Tifton, Georgia. Fifteen of the 65 hybrids were new hybrids developed using new technology (named YieldGard VecTran Triple, abbreviated as VT or VT3), which allows the stack of multiple traits for stalk protection, root protection and weed control in one transformation event. We have had a relatively dry corn growing season in 2008 which was favorable for the rapid buildup of all insect populations in the irrigated experimental plots. Seven ear-feeding insects were recorded as ear and kernel-feeding insect pests. They are in the order of infestation severity: the corn earworm and the fall armyworm, the southern green and brown stink bugs, the pink scavenger caterpillar and the sap beetles, and the maize weevil. Disease surveys around flowering time showed that smut, southern rust, and southern and northern blight infections were not a concern this year for all these hybrids. All entries were planted on April 4, and harvested in the week of September 2, 2008. Plots were thinned to 20,000 plants per acre. Total insect damage rating was based on ear-feeding insect (i.e., corn earworm and fall armyworm) damage rating and percentage of damaged kernels caused by the stink bugs, the pink scavenger caterpillar and the sap beetles, and the maize weevil. Overall insect resistance rating used five categories; they were very good (VG), good (G), fair (F), poor (P), and very poor (VP) as shown in the following table. While VG represents the lowest amount of insect damage, VP represents the greatest amount of insect damage. Losses to maize weevil, pink scavenger caterpillar and sap beetles were based on damage by multiple generations of these insects as the crop matures in the field. Corn earworm and fall armyworm damage was combined because the damage was hard to be separated; pink scavenger and sap beetle damage also was evaluated together. Corn earworm and fall armyworm feeding penetration in corn ears on the 65 hybrids was between 1.6 and 6.4 cm, which was higher than what we observed in 2007 (0.7 - 3.7 cm). It is worth noting that stink bug damage in 2008 (0.3-4.6% of discolored kernels) was higher than in either 2006 (0-1.5%) or 2007 (0.02-2%). Other insect damage was also higher in 2008 than in the previous two years. Pink scavenger caterpillar and sap beetle damage was 0.4-7.6% in 2008 compared to 0.1-2.6% in 2007 and 0.1-3.2% in 2006, and maize weevil damage was 0.04-3.8% in 2008, compared to 0.1-0.8% in 2007 and 0-0.6% in 2006. The most significant insect damage was still corn earworm and fall armyworm feeding on developing cobs, although increases in other insect damage were recorded. Only 2 of 15 new transgenic hybrids developed with VT or VT3 showed very good insect resistance, while 5 hybrids showed poor or very poor insect damage ratings. The findings suggested insect resistance expressions might vary among the hybrids. Timely harvest can substantially reduce losses caused by these two insects. Rankings of the 65 hybrids for their resistance to the major earand kernel-feeding insects (i.e., corn earworm and fall armyworm, pink scavenger caterpillar and sap beetles, maize weevil, and stink bugs) are given in the following table. The lettered ratings in the table refer only to relative resistance to insects and are not indicative of yield. Please refer to the yield data in other tests for specific information. During the damage evaluation in 2008, two physical features (i.e., husk tightness and husk extension) of corn ears were examined. Husk tightness was assigned using a scale of 1 to 5, in which 1 = very loose and 5 = very tight. Because average rating for husk tightness ranged between 3.8 and 4.6, the ratings were further categorized into three categories as loose (L), medium (M), and tight (T). Husk extension was shorter compared to previous years, ranging between 1.2 and 7.3 cm. The insect damage was not correlated to either of the husk features according to the data collected in 2008. Hybrids resistant to insects are highly recommended for planting and are presently the most economical means, especially in late plantings, for the reduction of ear-feeding insect damage. Consult your local county agent and/or extension entomologists for additional control recommendations for a specific insect pest in your region. Data for this report were collected by J. C. Mullis (USDA-ARS, Tifton, GA), Benjamin Berry, Justin Colbert, Cody Mauldin, and Austin Thompson (University of Georgia, Tifton, Georgia). Tifton, Georgia: Evaluations of Corn Hybrids for Insect Resistance, 2008 Overall Resistance to Insect Injury4 2 or more years 2008 Hybrid Name1 Maturity2 Days to Antheses Husk Extension cm Husk Tightness3 DeKalb Croplan Genetics Pioneer NK Croplan Genetics DKC69-71(RR2/YGCB) 851VT3 31N28(YGCB) N77-P5CB/LL 8702RR M M M S M 60 63 60 61 62 2.9 3.2 1.9 4 4.1 T M M M M VG VG VG VG VG G G G G G DeKalb Schillinger Golden Acres Croplan Genetics AgraTech DKC6640(YGCB) EX5066 28Z89 9009RR 7754 M M M M M 59 62 61 61 60 3.5 6.5 3.5 5.5 6.6 M L M T L VG VG VG VG VG . . . . . V5373 VT3 7891VT3 31P41 DKC66-23(RR2/YGCB) DKC67-23(RR2/YGCB) S M M M M 60 62 60 61 61 4.1 2.6 6.4 5.6 3.3 M M T L M VG G G G G . . G . G AgraTech Pioneer Croplan Genetics AgraTech Greenwood 695RR 33M57(HX/LL/RR2) 7505VT3 1777 TL3450RR S S S M M 61 62 59 61 61 4.5 3.2 5.8 4.8 4.1 M M M L M G G G G G G G GF G+ Golden Acres Croplan Genetics Schillinger Southern States Croplan Genetics 2989RRB 6831RH SX899 SS777VT3 8756RR/BT M S M M M 64 60 61 61 62 2.2 3.6 5.6 4.6 2.7 M M M M T G G G G G G G+ . . . Croplan Genetics Hytest Hytest Hytest Hytest 6886VT3 87-65RR/BT 87-20RH 68-13RH 90-19RR S M M S M 60 59 62 62 63 2.9 2.6 4.3 4 5.4 M M M M M G G G G G . . . . . AgraTech NK DynaGro DynaGro Greenwood EX8759 N78N-GT/CB/LL 58K81 V57YR82 EX3103RR M M M M M 59 60 61 60 62 4.5 2.9 2.4 5 2.7 M T M L M G G G G G . . . . . DynaGro Pioneer AgraTech Pioneer Pioneer CXO 7319 31D58 797RR 33V16(RR2/YGCB) 31G65(RR2) M M M S M 60 61 62 61 63 3.6 4.4 3.1 4.7 7.1 T M M M M G F F F F . F P GF- Company or Brand Name DynaGro Hytest Pioneer DeKalb DeKalb Tifton, Georgia: Evaluations of Corn Hybrids for Insect Resistance, 2008 (Continued) Overall Resistance to Insect Injury4 2 or more years 2008 Hybrid Name1 Maturity2 Days to Antheses Husk Extension cm Husk Tightness3 DKC67-87(RR2/YGCB) DKC69-40(VT3) DKC6458(YGCB) EX15133 SX896 M M S M M 61 59 59 61 63 4.3 3.9 4.5 7.3 2.2 M M M M M F F F F F . . . . . NG6811 NG6834 68-68VT3 7550VT3 751CBLL S S S S M 60 60 59 59 60 4.5 5.5 2.4 6.1 4.7 L L M M M F F F F F . . . . . AgraTech Garst DynaGro Greenwood Southern States EX8808 82R45GT 57V44 EX3280RR SS731CL M M S M S 61 59 60 62 61 5.2 2.6 4.7 2.4 3.9 M M M M M F F F F P . . . . P Southern States DeKalb Schillinger Fielder's Choice Southern States SS791CL DKC61-69(VT3) EX15135 NG6820 SS746VT3 M S S M S 63 60 62 59 62 1.6 3.6 5.4 1.2 3.2 M M M M M P P P P P . . . . . Hytest DynaGro DynaGro DeKalb Croplan Genetics 82-12VT3 CXO 7119 V60YR82 DKC63-42(VT3) 8221VT3 M M M S M 62 62 63 59 62 1.9 2.9 1.9 2.1 1.6 M T T L T P P P VP VP . . . . . Company or Brand Name DeKalb DeKalb DeKalb Schillinger Schillinger Fielder's Choice Direct Fielder's Choice Direct Hytest Hytest AgraTech All data in this table were collected from four replications. 1. The bolded entries have been examined for two or more years at Tifton, GA. 2. Maturity represents the hybrids categoried as short (S), medium (M), or long (L) season. Please note the Days to Anthesis (or flowering) data at Tifton, GA did not always support the categorization though. 3. L = loose husks, M = medium-tight husks, T = tight husks; 4. Overall insect resistance to ear-feeding insects (i.e., the corn earworm and the fall armyworm, the stink bugs, the pink scavenger caterpillar, and the maize weevil). Insect resistance measured by the ear-feeding insects from a total of 20 ears (five ears per replication) per hybrid, where VG = very good, G = good, F = fair, P = poor, and VP = very poor. The + and - signs for the average rating represent the inconsistancy among years.