Product datasheet
Anti-AMPK alpha 1 antibody - Aminoterminal end
ab49656
1 Image
Overview
Product name
Anti-AMPK alpha 1 antibody - Aminoterminal end
Description
Rabbit polyclonal to AMPK alpha 1 - Aminoterminal end
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide:
ATAEKQKHDGRVKIGHYI
conjugated to KLH, corresponding to N terminal amino acids 2-19 of Human AMPK alpha 1
Run BLAST with
Positive control
Run BLAST with
Whole cell extract of mouse 3T3-L1 adipocytes and a whole cell extract of human kidney 293 cell
line.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab49656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 60 kDa.
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Target
Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA
carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of
hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a
metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP
levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a
catalytic subunit.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
Anti-AMPK alpha 1 antibody - Aminoterminal end images
Lanes 1 - 3 : Anti-AMPK alpha 1 antibody Aminoterminal end (ab49656) at 1/4000
dilution
Lane 4 : Anti-AMPK alpha 1 antibody Aminoterminal end (ab49656) at 1/8000
dilution
Lane 5 : Anti-AMPK alpha 1 antibody Aminoterminal end (ab49656) at 1/10000
dilution
Lane 1 : undifferentiated embryonic 3T3-L1
Western blot - AMPK alpha 1 antibody (ab49656)
cells
Lane 2 : 3T3-L1 differentiated into
adipocytes by treatment with dexamethasone
and insulin. with Inhibition with AMPKa
(human 2-19) immunizing peptide. at 20 µg/ml
Lane 3 : 3T3-L1 differentiated into
adipocytes by treatment with dexamethasone
and insulin.
Lane 4 : 3T3-L1 differentiated into
adipocytes by treatment with dexamethasone
and insulin.
Lane 5 : 3T3-L1 differentiated into
adipocytes by treatment with dexamethasone
and insulin.
Secondary
HRP conjugated Goat Anti-Rabbit IgG
Predicted band size : 60 kDa
Observed band size : 60 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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