Kit Summary AB108653 Cancer Antigen CA125 Human ELISA Kit Product Code(s) Product name Enzyme Conjugate Reagent Chemical Name EC-No CAS-No Weight % Classification (67/548) Classification (Reg. 1272/2008) REACH Registration Number STOT SE 3 (H335) no data available Skin Irrit. 2 (H315) Eye Irrit. 2 (H319) Tris 201-064-4 77-86-1 0.61 Xi; R36/37/38 Proclin - 55965-84-9 0.5 T; R20/21/22 C; R34 R43 N; R50-53 Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) no data available Chemical Name EC-No CAS-No Weight % Classification (67/548) Classification (Reg. 1272/2008) REACH Registration Number TMB 259-364-6 54827-17-7 0.05 Xi; R36/37/38 STOT SE 3 (H335) Skin Irrit. 2 (H315) Eye Irrit. 2 (H319) Hydrogen peroxide 231-765-0 7722-84-1 0.02 Xn; R20/22 Acute Tox. 4 (H302) Acute Tox. 4 (H332) Skin Corr. 1A (H314) Eye Dam. 1 (H318) TMB Reagent C; R35 Xi; R41 R5 O; R8 Proclin - 55965-84-9 0.0015 T; R20/21/22 C; R34 R43 N; R50-53 Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) Chemical Name EC-No CAS-No Weight % Classification (67/548) Classification (Reg. 1272/2008) Hydrochloric acid 231-595-7 7647-01-0 3.5 Stop Solution C; R34 Xi; R37 CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 REACH Registration Number STOT SE 3 (H335) no data available Skin Corr. 1B (H314) Chemical Name EC-No CAS-No Weight % Classification (67/548) Classification (Reg. 1272/2008) Proclin - 55965-84-9 1 T; R20/21/22 C; R34 R43 N; R50-53 Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) REACH Registration Number no data available GHS / CLP - GHS - Classification Signal Word Danger Hazard statements H314 - Causes severe skin burns and eye damage H318 - Causes serious eye damage H317 - May cause an allergic skin reaction H412 - Harmful to aquatic life with long lasting effects Precautionary Statements - EU (§28, 1272/2008) P310 - Immediately call a POISON CENTER or doctor/ physician P301 + P330 + P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing P304 + P340 - IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing P303 + P361 + P353 - IF ON SKIN (or hair): Remove/ Take off immediately all contaminated clothing. Rinse skin with water/ shower P260 - Do not breathe dust/fume/gas/mist/vapors/spray P280 - Wear protective gloves/ eye protection/ face protection P405 - Store locked up P261 - Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P264 - Wash face, hands and any exposed skin thoroughly after handling P273 - Avoid release to the environment P272 - Contaminated work clothing should not be allowed out of the workplace P363 - Wash contaminated clothing before reuse P501 - Dispose of contents/ container to an approved waste disposal plant 2.3 Other information TRANSPORT INFORMATION DOT Not dangerous goods IATA Not dangerous goods ADR Not dangerous goods Safety Data Sheet Issuing date no data available Revision Date 12-Oct-2015 Version 3 1. PRODUCT AND COMPANY IDENTIFICATION Product name Enzyme Conjugate Reagent Recommended use For research use only Supplier Address Abcam Inc 1 Kendall Square, Ste 341 Cambridge, MA 02139-1517 USA Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) Fax: (866) 739-9884 or (866) 457-9616 (both US toll free) E-mail address technical@abcam.com Emergency telephone Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) - Monday-Friday 8am-9pm EST 2. HAZARDS IDENTIFICATION GHS - Classification Skin corrosion/irritation Serious eye damage/eye irritation Skin sensitization Chronic aquatic toxicity Ozone GHS Label elements, including precautionary statements Signal Word Warning Hazard statements H315 - Causes skin irritation H319 - Causes serious eye irritation H317 - May cause an allergic skin reaction H412 - Harmful to aquatic life with long lasting effects Category 2 Category 2 Category 1 Category 3 Not applicable Revision Date 12-Oct-2015 AB108653-CP2498 - Enzyme Conjugate Reagent _____________________________________________________________________________________________ Precautionary Statements - EU (§28, 1272/2008) P333 + P313 - If skin irritation or rash occurs: Get medical advice/ attention P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing P362 - Take off contaminated clothing and wash before reuse P337 + P313 - If eye irritation persists: Get medical advice/ attention P261 - Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P272 - Contaminated work clothing should not be allowed out of the workplace P280 - Wear protective gloves/ eye protection/ face protection P501 - Dispose of contents/ container to an approved waste disposal plant P264 - Wash face, hands and any exposed skin thoroughly after handling P273 - Avoid release to the environment Other information No information available 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substances Chemical Name Tris CAS-No 77-86-1 Weight % 0.1-1 Classification (Reg. 1272/2008) STOT SE 3 (H335) Skin Irrit. 2 (H315) Eye Irrit. 2 (H319) Proclin 55965-84-9 0.1-1 Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) For the full text of the H-Statements mentioned in this Section, see Section 16 4. FIRST AID MEASURES General advice If symptoms persist, call a physician. Eye contact Immediately flush with plenty of water. After initial flushing, remove any contact lenses and continue flushing for at least 15 minutes. If symptoms persist, call a physician. Skin contact Wash off immediately with soap and plenty of water while removing all contaminated clothes and shoes. If symptoms persist, call a physician. May cause an allergic skin reaction. Inhalation Move to fresh air. If symptoms persist, call a physician. Ingestion Rinse mouth. If symptoms persist, call a physician. Notes to physician May cause sensitization of susceptible persons. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Not flammable. _____________________________________________________________________________________________ Page 5 / 28 Revision Date 12-Oct-2015 AB108653-CP2498 - Enzyme Conjugate Reagent _____________________________________________________________________________________________ Flash point not determined Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Explosion Data Sensitivity to Mechanical Impact Sensitivity to Static Discharge none. none. Specific hazards arising from the chemical Thermal decomposition can lead to release of irritating gases and vapors In the event of fire and/or explosion do not breathe fumes May cause sensitization by inhalation and skin contact Protective equipment and precautions for firefighters As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. 6. ACCIDENTAL RELEASE MEASURES Personal precautions Use personal protective equipment. Avoid contact with the skin and the eyes. Evacuate personnel to safe areas. Keep people away from and upwind of spill/leak. Environmental precautions Prevent further leakage or spillage if safe to do so. Try to prevent the material from entering drains or water courses. Methods for containment Prevent further leakage or spillage if safe to do so. Methods for cleaning up Soak up with inert absorbent material. Pick up and transfer to properly labeled containers. After cleaning, flush away traces with water. Prevent product from entering drains. Dam up. 7. HANDLING AND STORAGE Advice on safe handling Ensure adequate ventilation. Wear personal protective equipment. Use only in area provided with appropriate exhaust ventilation. Do not breathe vapors or spray mist. Technical measures/Storage conditions Keep out of the reach of children. Keep container tightly closed. Keep containers tightly closed in a cool, well-ventilated place. Keep in properly labeled containers. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Exposure Guidelines This product does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies. Showers Eyewash stations Ventilation systems Personal protective equipment Eye/face protection Skin and body protection Respiratory protection Hygiene measures Tightly fitting safety goggles. Face-shield. No special protective equipment required. If exposure limits are exceeded or irritation is experienced, NIOSH/MSHA approved respiratory protection should be worn. Positive-pressure supplied air respirators may be required for high airborne contaminant concentrations. Respiratory protection must be provided in accordance with current local regulations. When using, do not eat, drink or smoke. Remove and wash contaminated clothing before re-use. Provide regular cleaning of equipment, work area and clothing. _____________________________________________________________________________________________ Page 6 / 28 Revision Date 12-Oct-2015 AB108653-CP2498 - Enzyme Conjugate Reagent _____________________________________________________________________________________________ 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor Threshold pH Flash point Decomposition temperature Melting point/range No information available. No information available No information available No information available No information available No information available Explosion limits No information available Specific Gravity Evaporation rate VOC Content(%) no data available No information available not applicable Odor Physical State @20°C No information available. No information available Autoignition temperature Boiling point/boiling range No information available No information available Flammability Limits in Air No information available solubility Vapor density No information available. no data available 10. STABILITY AND REACTIVITY Stability Stable under recommended storage conditions. Incompatible products None known based on information supplied. Conditions to avoid None known based on information supplied. Hazardous decomposition products None known based on information supplied. Hazardous polymerization Hazardous polymerization does not occur. 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Irritating to skin, eyes and / or respiratory tract. May cause sensitization of susceptible persons. Inhalation May cause irritation of respiratory tract Eye contact May cause irritation Skin contact May cause irritation Ingestion Ingestion may cause irritation to mucous membranes Chemical Name Tris LD50 Oral 5900 mg/kg ( Rat ) Proclin 53 mg/kg ( Rat ) LD50 Dermal LC50 Inhalation Chronic toxicity Chronic toxicity Repeated contact may cause allergic reactions in very susceptible persons Avoid repeated exposure Sensitization As a precaution the product should be treated as a sensitizer. Target Organ Effects None known. _____________________________________________________________________________________________ Page 7 / 28 Revision Date 12-Oct-2015 AB108653-CP2498 - Enzyme Conjugate Reagent _____________________________________________________________________________________________ 12. ECOLOGICAL INFORMATION Ecotoxicity Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. 13. DISPOSAL CONSIDERATIONS Waste disposal methods This material, as supplied, is not a hazardous waste according to Federal regulations (40 Contaminated packaging Do not re-use empty containers. 14. TRANSPORT INFORMATION DOT Not dangerous goods IATA Not dangerous goods ADR Not dangerous goods 15. REGULATORY INFORMATION U.S. Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product does not contain any chemicals which are subject to the reporting requirements of the Act and Title 40 of the Code of Federal Regulations, Part 372. SARA 311/312 Hazard Categories Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard yes no no no no Clean Water Act This product does not contain any substances regulated as pollutants pursuant to the Clean Water Act (40 CFR 122.21 and 40 CFR 122.42). CERCLA This material, as supplied, does not contain any substances regulated as hazardous substances under the Comprehensive Environmental Response Compensation and Liability Act (CERCLA) (40 CFR 302) or the Superfund Amendments and Reauthorization Act (SARA) (40 CFR 355). There may be specific reporting requirements at the local, regional, or state level pertaining to releases of this material. U.S. State Regulations California Proposition 65 This product does not contain any Proposition 65 chemicals. _____________________________________________________________________________________________ Page 8 / 28 Revision Date 12-Oct-2015 AB108653-CP2498 - Enzyme Conjugate Reagent _____________________________________________________________________________________________ U.S. State Right-to-Know Regulations International Regulations WHMIS Note: This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. Full text of H-Statements referred to under sections 2 and 3 H314 - Causes severe skin burns and eye damage H317 - May cause an allergic skin reaction H400 - Very toxic to aquatic life H410 - Very toxic to aquatic life with long lasting effects Hazard statements H315 - Causes skin irritation H319 - Causes serious eye irritation H317 - May cause an allergic skin reaction H412 - Harmful to aquatic life with long lasting effects Revision Note No information available. Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal End of MSDS _____________________________________________________________________________________________ Safety Data Sheet Issuing date no data available Revision Date 12-Oct-2015 Version 2 1. PRODUCT AND COMPANY IDENTIFICATION Product name TMB Reagent Recommended use For research use only Supplier Address Abcam Inc 1 Kendall Square, Ste 341 Cambridge, MA 02139-1517 USA Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) Fax: (866) 739-9884 or (866) 457-9616 (both US toll free) E-mail address technical@abcam.com Emergency telephone Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) - Monday-Friday 8am-9pm EST 2. HAZARDS IDENTIFICATION GHS - Classification Skin sensitization Ozone GHS Label elements, including precautionary statements Signal Word Warning Hazard statements H317 - May cause an allergic skin reaction Precautionary Statements - EU (§28, 1272/2008) P302 + P352 - IF ON SKIN: Wash with plenty of soap and water P333 + P313 - If skin irritation or rash occurs: Get medical advice/ attention P363 - Wash contaminated clothing before reuse P261 - Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P272 - Contaminated work clothing should not be allowed out of the workplace P280 - Wear protective gloves/ eye protection/ face protection P501 - Dispose of contents/ container to an approved waste disposal plant Other information Category 1 Not applicable Revision Date 12-Oct-2015 AB108653-CP2501 - TMB Reagent _____________________________________________________________________________________________ No information available 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substances Chemical Name TMB CAS-No 54827-17-7 Weight % <0.1 Classification (Reg. 1272/2008) STOT SE 3 (H335) Skin Irrit. 2 (H315) Eye Irrit. 2 (H319) Hydrogen peroxide 7722-84-1 <0.1 Acute Tox. 4 (H302) Acute Tox. 4 (H332) Skin Corr. 1A (H314) Eye Dam. 1 (H318) Proclin 55965-84-9 <0.1 Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) For the full text of the H-Statements mentioned in this Section, see Section 16 4. FIRST AID MEASURES General advice If symptoms persist, call a physician. Eye contact Immediately flush with plenty of water. After initial flushing, remove any contact lenses and continue flushing for at least 15 minutes. Skin contact Wash off immediately with soap and plenty of water while removing all contaminated clothes and shoes. May cause an allergic skin reaction. If symptoms persist, call a physician. Inhalation Move to fresh air. Ingestion Clean mouth with water. Drink plenty of water. Notes to physician May cause sensitization of susceptible persons. Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Not flammable. Flash point not determined Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Explosion Data Sensitivity to Mechanical Impact Sensitivity to Static Discharge none. none. _____________________________________________________________________________________________ Page 11 / 28 Revision Date 12-Oct-2015 AB108653-CP2501 - TMB Reagent _____________________________________________________________________________________________ Specific hazards arising from the chemical Thermal decomposition can lead to release of irritating gases and vapors In the event of fire and/or explosion do not breathe fumes May cause sensitization by inhalation and skin contact Protective equipment and precautions for firefighters As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. 6. ACCIDENTAL RELEASE MEASURES Personal precautions Use personal protective equipment. Avoid contact with the skin and the eyes. Environmental precautions Try to prevent the material from entering drains or water courses. Methods for containment Prevent further leakage or spillage if safe to do so. Methods for cleaning up Soak up with inert absorbent material. Pick up and transfer to properly labeled containers. After cleaning, flush away traces with water. Prevent product from entering drains. 7. HANDLING AND STORAGE Advice on safe handling Ensure adequate ventilation. Wear personal protective equipment. Technical measures/Storage conditions Keep out of the reach of children. Keep container tightly closed. Keep containers tightly closed in a cool, well-ventilated place. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Exposure Guidelines Chemical Name Hydrogen peroxide 7722-84-1 This product does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies. ACGIH TLV TWA: 1 ppm OSHA PEL TWA: 1 ppm TWA: 1.4 mg/m3 (vacated) TWA: 1 ppm (vacated) TWA: 1.4 mg/m3 NIOSH IDLH IDLH: 75 ppm TWA: 1 ppm TWA: 1.4 mg/m3 NIOSH IDLH: Immediately Dangerous to Life or Health Other Exposure Guidelines Vacated limits revoked by the Court of Appeals decision in AFL-CIO v. OSHA, 965 F.2d 962 (11th Cir., 1992). Engineering measures Showers Eyewash stations Ventilation systems Personal protective equipment Eye/face protection Skin and body protection Respiratory protection Hygiene measures Tightly fitting safety goggles. No special protective equipment required. If exposure limits are exceeded or irritation is experienced, NIOSH/MSHA approved respiratory protection should be worn. Positive-pressure supplied air respirators may be required for high airborne contaminant concentrations. Respiratory protection must be provided in accordance with current local regulations. When using, do not eat, drink or smoke. Remove and wash contaminated clothing before re-use. _____________________________________________________________________________________________ Page 12 / 28 Revision Date 12-Oct-2015 AB108653-CP2501 - TMB Reagent _____________________________________________________________________________________________ 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor Threshold pH Flash point Decomposition temperature Melting point/range No information available. No information available No information available No information available No information available No information available Explosion limits No information available Specific Gravity Evaporation rate VOC Content(%) no data available No information available not applicable Odor Physical State @20°C No information available. No information available Autoignition temperature Boiling point/boiling range No information available No information available Flammability Limits in Air No information available solubility Vapor density No information available. no data available 10. STABILITY AND REACTIVITY Stability Stable under recommended storage conditions. Incompatible products None known based on information supplied. Conditions to avoid None known based on information supplied. Hazardous decomposition products None known based on information supplied. Hazardous polymerization Hazardous polymerization does not occur. 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information May cause sensitization of susceptible persons. Chemical Name Hydrogen peroxide LD50 Oral 801 mg/kg ( Rat ) Proclin 53 mg/kg ( Rat ) LD50 Dermal 4060 mg/kg ( Rat ) 2000 mg/kg ( Rabbit ) LC50 Inhalation 2 mg/L ( Rat ) 4 h Chronic toxicity Chronic toxicity Repeated contact may cause allergic reactions in very susceptible persons Avoid repeated exposure Carcinogenicity This product contains one or more substances which are classified by IARC as carcinogenic to humans (Group I), probably carcinogenic to humans (Group 2A) or possibly carcinogenic to humans (Group 2B) Chemical Name Hydrogen peroxide ACGIH A3 IARC Group 3 NTP Sensitization As a precaution the product should be treated as a sensitizer. Target Organ Effects None known. OSHA _____________________________________________________________________________________________ Page 13 / 28 Revision Date 12-Oct-2015 AB108653-CP2501 - TMB Reagent _____________________________________________________________________________________________ 12. ECOLOGICAL INFORMATION Ecotoxicity The environmental impact of this product has not been fully investigated. Chemical Name Toxicity to algae Hydrogen peroxide Toxicity to fish Toxicity to microorganisms 2.5: 72 h Chlorella vulgaris 16.4: 96 h Pimephales mg/L EC50 promelas mg/L LC50 18-56: 96 h Lepomis macrochirus mg/L LC50 static 10.0-32.0: 96 h Oncorhynchus mykiss mg/L LC50 static Toxicity to daphnia and other aquatic invertebrates 7.7: 24 h Daphnia magna mg/L EC50 18 - 32: 48 h Daphnia magna mg/L EC50 Static 13. DISPOSAL CONSIDERATIONS Waste disposal methods This material, as supplied, is not a hazardous waste according to Federal regulations (40 CFR 261). This material could become a hazardous waste if it is mixed with or otherwise comes in contact with a hazardous waste, if chemical additions are made to this material, or if the material is processed or otherwise altered. Consult 40 CFR 261 to determine whether the altered material is a hazardous waste. Consult the appropriate state, regional, or local regulations for additional requirements. Contaminated packaging Do not re-use empty containers. This product contains one or more substances that are listed with the State of California as a hazardous waste. Chemical Name Hydrogen peroxide California Hazardous Waste Status Toxic Corrosive Ignitable Reactive 14. TRANSPORT INFORMATION DOT Not dangerous goods IATA Not dangerous goods ADR Not dangerous goods 15. REGULATORY INFORMATION U.S. Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product does not contain any chemicals which are subject to the reporting requirements of the Act and Title 40 of the Code of Federal Regulations, Part 372. SARA 311/312 Hazard Categories Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard yes no no no no Clean Water Act This product does not contain any substances regulated as pollutants pursuant to the Clean Water Act (40 CFR 122.21 and 40 CFR 122.42). _____________________________________________________________________________________________ Page 14 / 28 Revision Date 12-Oct-2015 AB108653-CP2501 - TMB Reagent _____________________________________________________________________________________________ CERCLA This material, as supplied, does not contain any substances regulated as hazardous substances under the Comprehensive Environmental Response Compensation and Liability Act (CERCLA) (40 CFR 302) or the Superfund Amendments and Reauthorization Act (SARA) (40 CFR 355). There may be specific reporting requirements at the local, regional, or state level pertaining to releases of this material. Chemical Name Hazardous Substances RQs Extremely Hazardous Substances RQs 1000 lb Hydrogen peroxide RQ U.S. State Regulations California Proposition 65 This product does not contain any Proposition 65 chemicals. U.S. State Right-to-Know Regulations Chemical Name Hydrogen peroxide Massachusetts X New Jersey X Pennsylvania X Illinois Rhode Island X International Regulations Chemical Name Hydrogen peroxide WHMIS Note: Carcinogen Status A3 Exposure limits Mexico: TWA 1 ppm Mexico: TWA 1.5 mg/m3 Mexico: STEL 2 ppm Mexico: STEL 3 mg/m3 This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. 16. OTHER INFORMATION Full text of H-Statements referred to under sections 2 and 3 H314 - Causes severe skin burns and eye damage H317 - May cause an allergic skin reaction H400 - Very toxic to aquatic life H410 - Very toxic to aquatic life with long lasting effects H302 - Harmful if swallowed H332 - Harmful if inhaled H318 - Causes serious eye damage Hazard statements H317 - May cause an allergic skin reaction 12-Oct-2015 Revision Date No information available. Revision Note Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. End of MSDS _____________________________________________________________________________________________ Page 15 / 28 Safety Data Sheet Issuing date no data available Revision Date 12-Oct-2015 Version 3 1. PRODUCT AND COMPANY IDENTIFICATION Product name Stop Solution Recommended use For research use only Supplier Address Abcam Inc 1 Kendall Square, Ste 341 Cambridge, MA 02139-1517 USA Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) Fax: (866) 739-9884 or (866) 457-9616 (both US toll free) E-mail address technical@abcam.com Emergency telephone Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) - Monday-Friday 8am-9pm EST 2. HAZARDS IDENTIFICATION GHS - Classification Ozone Not applicable GHS Label elements, including precautionary statements Other information No information available 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substances Chemical Name Hydrochloric acid CAS-No 7647-01-0 Weight % 1-5 For the full text of the H-Statements mentioned in this Section, see Section 16 Classification (Reg. 1272/2008) STOT SE 3 (H335) Skin Corr. 1B (H314) Revision Date 12-Oct-2015 AB108653-CP2502 - Stop Solution _____________________________________________________________________________________________ 4. FIRST AID MEASURES Eye contact Immediately flush with plenty of water. After initial flushing, remove any contact lenses and continue flushing for at least 15 minutes. Skin contact Wash off immediately with soap and plenty of water while removing all contaminated clothes and shoes. Inhalation Move to fresh air. Ingestion Clean mouth with water. Drink plenty of water. Notes to physician Treat symptomatically. 5. FIRE-FIGHTING MEASURES Flammable properties Not flammable. Flash point not determined Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Explosion Data Sensitivity to Mechanical Impact Sensitivity to Static Discharge none. none. Protective equipment and precautions for firefighters As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. 6. ACCIDENTAL RELEASE MEASURES Personal precautions Ensure adequate ventilation. Environmental precautions Try to prevent the material from entering drains or water courses. Methods for containment Prevent further leakage or spillage if safe to do so. Methods for cleaning up Pick up and transfer to properly labeled containers. 7. HANDLING AND STORAGE Advice on safe handling Handle in accordance with good industrial hygiene and safety practice. Technical measures/Storage conditions Keep containers tightly closed in a dry, cool and well-ventilated place. _____________________________________________________________________________________________ Page 17 / 28 Revision Date 12-Oct-2015 AB108653-CP2502 - Stop Solution _____________________________________________________________________________________________ 8. EXPOSURE CONTROLS/PERSONAL PROTECTION This product does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies. Exposure Guidelines Chemical Name Hydrochloric acid 7647-01-0 ACGIH TLV Ceiling: 2 ppm OSHA PEL (vacated) Ceiling: 5 ppm (vacated) Ceiling: 7 mg/m3 Ceiling: 5 ppm Ceiling: 7 mg/m3 NIOSH IDLH IDLH: 50 ppm Ceiling: 5 ppm Ceiling: 7 mg/m3 NIOSH IDLH: Immediately Dangerous to Life or Health Other Exposure Guidelines Vacated limits revoked by the Court of Appeals decision in AFL-CIO v. OSHA, 965 F.2d 962 (11th Cir., 1992). Engineering measures Showers Eyewash stations Ventilation systems Personal protective equipment Eye/face protection Skin and body protection Respiratory protection Hygiene measures No special protective equipment required. No special protective equipment required. If exposure limits are exceeded or irritation is experienced, NIOSH/MSHA approved respiratory protection should be worn. Positive-pressure supplied air respirators may be required for high airborne contaminant concentrations. Respiratory protection must be provided in accordance with current local regulations. Handle in accordance with good industrial hygiene and safety practice. 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor Threshold pH Flash point Decomposition temperature Melting point/range No information available. No information available No information available No information available No information available No information available Explosion limits No information available Specific Gravity Evaporation rate VOC Content(%) no data available No information available not applicable Odor Physical State @20°C No information available. No information available Autoignition temperature Boiling point/boiling range No information available No information available Flammability Limits in Air No information available solubility Vapor density No information available. no data available 10. STABILITY AND REACTIVITY Stability Stable under recommended storage conditions. Incompatible products None known based on information supplied. Conditions to avoid None known based on information supplied. Hazardous decomposition products None known based on information supplied. Hazardous polymerization Hazardous polymerization does not occur. _____________________________________________________________________________________________ Page 18 / 28 Revision Date 12-Oct-2015 AB108653-CP2502 - Stop Solution _____________________________________________________________________________________________ 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Chemical Name Hydrochloric acid Product does not present an acute toxicity hazard based on known or supplied information. LD50 Oral 700 mg/kg ( Rat ) LD50 Dermal 5010 mg/kg ( Rabbit ) LC50 Inhalation 3124 ppm ( Rat ) 1 h Chronic toxicity Carcinogenicity Chemical Name Hydrochloric acid This product contains one or more substances which are classified by IARC as carcinogenic to humans (Group I), probably carcinogenic to humans (Group 2A) or possibly carcinogenic to humans (Group 2B) ACGIH IARC Group 3 NTP OSHA Toxicity to microorganisms Toxicity to daphnia and other aquatic invertebrates IARC: (International Agency for Research on Cancer) Group 3 - Not Classifiable as to Carcinogenicity in Humans Target Organ Effects Eyes. Respiratory system. Skin. 12. ECOLOGICAL INFORMATION Ecotoxicity The environmental impact of this product has not been fully investigated. Chemical Name Hydrochloric acid Toxicity to algae Toxicity to fish 282: 96 h Gambusia affinis mg/L LC50 static 13. DISPOSAL CONSIDERATIONS Waste disposal methods This material, as supplied, is not a hazardous waste according to Federal regulations (40 CFR 261). This material could become a hazardous waste if it is mixed with or otherwise comes in contact with a hazardous waste, if chemical additions are made to this material, or if the material is processed or otherwise altered. Consult 40 CFR 261 to determine whether the altered material is a hazardous waste. Consult the appropriate state, regional, or local regulations for additional requirements. Contaminated packaging Do not re-use empty containers. 14. TRANSPORT INFORMATION DOT Not dangerous goods IATA Not dangerous goods ADR Not dangerous goods 15. REGULATORY INFORMATION U.S. Federal Regulations _____________________________________________________________________________________________ Page 19 / 28 Revision Date 12-Oct-2015 AB108653-CP2502 - Stop Solution _____________________________________________________________________________________________ SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product contains a chemical or chemicals which are subject to the reporting requirements of the Act and Title 40 of the Code of Federal Regulations, Part 372: Chemical Name CAS-No Weight % Hydrochloric acid 7647-01-0 3.5 SARA 311/312 Hazard Categories Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard SARA 313 - Threshold Values % 1.0 no no no no no Clean Water Act This product does not contain any substances regulated as pollutants pursuant to the Clean Water Act (40 CFR 122.21 and 40 CFR 122.42). Chemical Name CWA - Reportable Quantities 5000 lb Hydrochloric acid CWA - Toxic Pollutants CWA - Priority Pollutants CWA - Hazardous Substances X CERCLA This material, as supplied, does not contain any substances regulated as hazardous substances under the Comprehensive Environmental Response Compensation and Liability Act (CERCLA) (40 CFR 302) or the Superfund Amendments and Reauthorization Act (SARA) (40 CFR 355). There may be specific reporting requirements at the local, regional, or state level pertaining to releases of this material. Chemical Name Hazardous Substances RQs Hydrochloric acid 5000 lb Extremely Hazardous Substances RQ RQs 5000 lb RQ 5000 lb final RQ RQ 2270 kg final RQ U.S. State Regulations California Proposition 65 This product does not contain any Proposition 65 chemicals. U.S. State Right-to-Know Regulations Chemical Name Hydrochloric acid Massachusetts X New Jersey X Pennsylvania X Illinois X Rhode Island X International Regulations Chemical Name Hydrochloric acid WHMIS Note: Carcinogen Status Exposure limits Mexico: Ceiling 5 ppm Mexico: Ceiling 7 mg/m3 This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. _____________________________________________________________________________________________ Page 20 / 28 Revision Date 12-Oct-2015 AB108653-CP2502 - Stop Solution _____________________________________________________________________________________________ 16. OTHER INFORMATION Full text of H-Statements referred to under sections 2 and 3 H335 - May cause respiratory irritation H314 - Causes severe skin burns and eye damage Revision Date Revision Note 12-Oct-2015 No information available. Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. End of MSDS _____________________________________________________________________________________________ Page 21 / 28 Safety Data Sheet Issuing date no data available Revision Date 12-Oct-2015 Version 4 1. PRODUCT AND COMPANY IDENTIFICATION Product name CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 Recommended use For research use only Supplier Address Abcam Inc 1 Kendall Square, Ste 341 Cambridge, MA 02139-1517 USA Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) Fax: (866) 739-9884 or (866) 457-9616 (both US toll free) E-mail address technical@abcam.com Emergency telephone Tel: (617)225-2272 or 888-77-ABCAM (22226) (US toll free) - Monday-Friday 8am-9pm EST 2. HAZARDS IDENTIFICATION GHS - Classification Skin corrosion/irritation Serious eye damage/eye irritation Skin sensitization Chronic aquatic toxicity Ozone Category 1 Category 1 Category 1 Category 3 Not applicable GHS Label elements, including precautionary statements Signal Word Danger Hazard statements H314 - Causes severe skin burns and eye damage H318 - Causes serious eye damage H317 - May cause an allergic skin reaction H412 - Harmful to aquatic life with long lasting effects _____________________________________________________________________________________________ Page 22 / 28 Revision Date 12-Oct-2015 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 _____________________________________________________________________________________________ Precautionary Statements - EU (§28, 1272/2008) P310 - Immediately call a POISON CENTER or doctor/ physician P301 + P330 + P331 - IF SWALLOWED: rinse mouth. Do NOT induce vomiting P305 + P351 + P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing P304 + P340 - IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing P303 + P361 + P353 - IF ON SKIN (or hair): Remove/ Take off immediately all contaminated clothing. Rinse skin with water/ shower P260 - Do not breathe dust/fume/gas/mist/vapors/spray P280 - Wear protective gloves/ eye protection/ face protection P405 - Store locked up P261 - Avoid breathing dust/ fume/ gas/ mist/ vapors/ spray P264 - Wash face, hands and any exposed skin thoroughly after handling P273 - Avoid release to the environment P272 - Contaminated work clothing should not be allowed out of the workplace P363 - Wash contaminated clothing before reuse P501 - Dispose of contents/ container to an approved waste disposal plant Other information No information available 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1 Substances Chemical Name Proclin CAS-No 55965-84-9 Weight % 1-5 Classification (Reg. 1272/2008) Skin Corr. 1B (H314) Skin Sens. 1 (H317) Aquatic Acute 1 (H400) Aquatic Chronic 1 (H410) For the full text of the H-Statements mentioned in this Section, see Section 16 _____________________________________________________________________________________________ Page 23 / 28 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 Revision Date 12-Oct-2015 _____________________________________________________________________________________________ 4. FIRST AID MEASURES General advice Immediate medical attention is required. If symptoms persist, call a physician. Eye contact Immediately flush with plenty of water. After initial flushing, remove any contact lenses and continue flushing for at least 15 minutes. Immediate medical attention is required. Skin contact Wash off immediately with soap and plenty of water while removing all contaminated clothes and shoes. Immediate medical attention is required. May cause an allergic skin reaction. If symptoms persist, call a physician. Inhalation Move to fresh air. Avoid direct contact with skin. Use barrier to give mouth-to-mouth resuscitation. Immediate medical attention is required. Ingestion Rinse mouth. Do NOT induce vomiting. Immediate medical attention is required. Notes to physician Product is a corrosive material. Use of gastric lavage or emesis is contraindicated. Possible perforation of stomach or esophagus should be investigated. Do not give chemical antidotes. Asphyxia from glottal edema may occur. Marked decrease in blood pressure may occur with moist rales, frothy sputum, and high pulse pressure. Treat symptomatically. May cause sensitization of susceptible persons. Protection of first-aiders Use personal protective equipment. Ensure that medical personnel are aware of the material(s) involved, and take precautions to protect themselves. 5. FIRE-FIGHTING MEASURES Flammable properties Not flammable. Flash point not determined Suitable extinguishing media Use extinguishing measures that are appropriate to local circumstances and the surrounding environment. Explosion Data Sensitivity to Mechanical Impact Sensitivity to Static Discharge none. none. Specific hazards arising from the chemical The product causes burns of eyes, skin and mucous membranes Thermal decomposition can lead to release of irritating gases and vapors In the event of fire and/or explosion do not breathe fumes Protective equipment and precautions for firefighters As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full protective gear. _____________________________________________________________________________________________ Page 24 / 28 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 Revision Date 12-Oct-2015 _____________________________________________________________________________________________ 6. ACCIDENTAL RELEASE MEASURES Avoid contact with skin, eyes and clothing. Use personal protective equipment. Evacuate personnel to safe areas. Keep people away from and upwind of spill/leak. Environmental precautions Prevent further leakage or spillage if safe to do so. Try to prevent the material from entering drains or water courses. Methods for containment Prevent further leakage or spillage if safe to do so. Methods for cleaning up Take up mechanically and collect in suitable container for disposal. Dike far ahead of liquid spill for later disposal. Soak up with inert absorbent material. Clean contaminated surface thoroughly. Prevent product from entering drains. Dam up. After cleaning, flush away traces with water. Other information Refer to protective measures listed in sections 7 and 8. 7. HANDLING AND STORAGE Advice on safe handling Avoid contact with skin, eyes and clothing. Wear personal protective equipment. Ensure adequate ventilation. In case of insufficient ventilation, wear suitable respiratory equipment. Handle product only in closed system or provide appropriate exhaust ventilation at machinery. Technical measures/Storage conditions Keep container tightly closed in a dry and well-ventilated place. Keep out of the reach of children. Keep containers tightly closed in a cool, well-ventilated place. Keep containers tightly closed in a dry, cool and well-ventilated place. Keep in properly labeled containers. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Exposure Guidelines This product does not contain any hazardous materials with occupational exposure limits established by the region specific regulatory bodies. Engineering measures Showers Eyewash stations Ventilation systems Personal protective equipment Eye/face protection Skin and body protection Respiratory protection Hygiene measures Tightly fitting safety goggles. Face-shield. No special protective equipment required. If exposure limits are exceeded or irritation is experienced, NIOSH/MSHA approved respiratory protection should be worn. Positive-pressure supplied air respirators may be required for high airborne contaminant concentrations. Respiratory protection must be provided in accordance with current local regulations. When using, do not eat, drink or smoke. Remove and wash contaminated clothing before re-use. Keep away from food, drink and animal feeding stuffs. Contaminated work clothing should not be allowed out of the workplace. Provide regular cleaning of equipment, work area and clothing. Avoid contact with skin, eyes and clothing. For environmental protection, remove and wash all contaminated protective equipment before re-use. Wear suitable gloves and eye/face protection. _____________________________________________________________________________________________ Page 25 / 28 Revision Date 12-Oct-2015 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 _____________________________________________________________________________________________ 9. PHYSICAL AND CHEMICAL PROPERTIES Appearance Odor Threshold pH Flash point Decomposition temperature Melting point/range No information available. No information available No information available No information available No information available No information available Explosion limits No information available Specific Gravity Evaporation rate VOC Content(%) no data available No information available not applicable Odor Physical State @20°C No information available. No information available Autoignition temperature Boiling point/boiling range No information available No information available Flammability Limits in Air No information available solubility Vapor density No information available. no data available 10. STABILITY AND REACTIVITY Stability Stable under recommended storage conditions. Incompatible products Incompatible with strong acids and bases. Incompatible with oxidizing agents. Conditions to avoid Exposure to air or moisture over prolonged periods. Hazardous decomposition products Thermal decomposition can lead to release of irritating gases and vapors. Hazardous polymerization Hazardous polymerization does not occur. 11. TOXICOLOGICAL INFORMATION Acute toxicity Product Information Corrosive to skin, eyes and/or respiratory tract. May cause sensitization of susceptible persons. Inhalation Corrosive by inhalation Causes burns Eye contact Causes burns Corrosive to eyes Skin contact Corrosive Causes burns Ingestion Causes burns Chronic toxicity Chronic toxicity Chronic exposure to corrosive fumes/gases may cause erosion of the teeth followed by jaw necrosis. Bronchial irritation with chronic cough and frequent attacks of pneumonia are common. Gastrointestinal disturbances may also be seen Avoid repeated exposure Possible risks of irreversible effects Sensitization As a precaution the product should be treated as a sensitizer. Target Organ Effects None known. _____________________________________________________________________________________________ Page 26 / 28 Revision Date 12-Oct-2015 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 _____________________________________________________________________________________________ 12. ECOLOGICAL INFORMATION Ecotoxicity Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. 13. DISPOSAL CONSIDERATIONS Waste disposal methods Contaminated packaging Do not re-use empty containers. 14. TRANSPORT INFORMATION DOT Not dangerous goods IATA Not dangerous goods ADR Not dangerous goods 15. REGULATORY INFORMATION U.S. Federal Regulations SARA 313 Section 313 of Title III of the Superfund Amendments and Reauthorization Act of 1986 (SARA). This product does not contain any chemicals which are subject to the reporting requirements of the Act and Title 40 of the Code of Federal Regulations, Part 372. SARA 311/312 Hazard Categories Acute Health Hazard Chronic Health Hazard Fire Hazard Sudden Release of Pressure Hazard Reactive Hazard yes yes no no no Clean Water Act This product does not contain any substances regulated as pollutants pursuant to the Clean Water Act (40 CFR 122.21 and 40 CFR 122.42). CERCLA This material, as supplied, does not contain any substances regulated as hazardous substances under the Comprehensive Environmental Response Compensation and Liability Act (CERCLA) (40 CFR 302) or the Superfund Amendments and Reauthorization Act (SARA) (40 CFR 355). There may be specific reporting requirements at the local, regional, or state level pertaining to releases of this material. U.S. State Regulations California Proposition 65 This product does not contain any Proposition 65 chemicals. _____________________________________________________________________________________________ Page 27 / 28 AB108653-CP91363 - CA125 reference standards containing; 0, 15, 50, 100, 200, and 400 Unit/ml of CA125 Revision Date 12-Oct-2015 _____________________________________________________________________________________________ U.S. State Right-to-Know Regulations International Regulations WHMIS Note: This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations (CPR) and the MSDS contains all the information required by the CPR. 16. OTHER INFORMATION Full text of H-Statements referred to under sections 2 and 3 H314 - Causes severe skin burns and eye damage H317 - May cause an allergic skin reaction H400 - Very toxic to aquatic life H410 - Very toxic to aquatic life with long lasting effects Hazard statements H314 - Causes severe skin burns and eye damage H318 - Causes serious eye damage H317 - May cause an allergic skin reaction H412 - Harmful to aquatic life with long lasting effects 12-Oct-2015 Revision Date No information available. Revision Note Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its and release and is not to be considered a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text. End of MSDS _____________________________________________________________________________________________ Page 28 / 28