Product datasheet
Anti-Glycogen synthase 1 antibody [EP817Y] (HRP)
ab202546
2 Images
Overview
Product name
Anti-Glycogen synthase 1 antibody [EP817Y] (HRP)
Description
Rabbit monoclonal [EP817Y] to Glycogen synthase 1 (HRP)
Conjugation
HRP
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Human Glycogen Synthase.
Positive control
WB: Hela whole cell lysate. IHC-P: FFPE human skeletal muscle (normal) tissue sections.
General notes
Alternative versions available:
Anti-Glycogen synthase 1 antibody [EP817Y] (ab40810)
Anti-Glycogen synthase 1 antibody (Alexa Fluor® 488) [EP817Y] (ab202759)
Anti-Glycogen synthase 1 antibody (Alexa Fluor® 647) [EP817Y] (ab202761)
Anti-Glycogen synthase 1 antibody (Phycoerythrin) [EP817Y] (ab210583)
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EP817Y
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab202546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 85 kDa (predicted molecular weight: 81
kDa).
Target
Function
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Pathway
Glycan biosynthesis; glycogen biosynthesis.
Involvement in disease
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b)
[MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic
disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role
of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle
work.
Sequence similarities
Belongs to the glycosyltransferase 3 family.
Anti-Glycogen synthase 1 antibody [EP817Y] (HRP) images
2
IHC image of Glycogen synthase 1 staining in
a section of formalin-fixed paraffin-embedded
normal human skeletal muscle*. The section
was pre-treated using pressure cooker heat
mediated antigen retrieval with sodium citrate
buffer (pH6) for 30mins, and incubated
overnight at +4°C with ab202546 at 1/100
dilution. DAB was used as the chromogen
(ab103723), diluted 1/100 and incubated for
10min at room temperature. The section was
counterstained with haematoxylin and
Immunohistochemistry (Formalin/PFA-fixed
mounted with DPX. The inset negative control
paraffin-embedded sections) - Anti-Glycogen
image is taken from an identical assay
synthase 1 antibody [EP817Y] (HRP) (ab202546)
without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
3
Anti-Glycogen synthase 1 antibody [EP817Y]
(HRP) (ab202546) at 1/5000 dilution + HeLa
(Human epithelial carcinoma cell line) Whole
Cell Lysate at 10 µg
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 81 kDa
Observed band size : 85 kDa
Western blot - Anti-Glycogen synthase 1 antibody
[EP817Y] (HRP) (ab202546)
Exposure time : 4 minutes
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 3% milk before being incubated with
ab202546 overnight at 4°C. Antibody binding
was visualised using ECL development
solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
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