Product datasheet
Anti-Glycogen synthase 1 antibody [EP817Y] ab40810
3 Abreviews 3 References 3 Images
Overview
Product name
Anti-Glycogen synthase 1 antibody [EP817Y]
Description
Rabbit monoclonal [EP817Y] to Glycogen synthase 1
Tested applications
IHC-P, WB, ICC/IF, Flow Cyt, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Human Glycogen Synthase.
Positive control
Hela cell lysate.
General notes
This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb®
technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or
7,429,487.
Alternative versions available:
Anti-Glycogen synthase 1 antibody (Alexa Fluor® 488) [EP817Y] (ab202759)
Anti-Glycogen synthase 1 antibody (Alexa Fluor® 647) [EP817Y] (ab202761)
Anti-Glycogen synthase 1 antibody (HRP) [EP817Y] (ab202546)
Anti-Glycogen synthase 1 antibody (Phycoerythrin) [EP817Y] (ab210583)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EP817Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab40810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
IHC-P
Use at an assay dependent concentration.
WB
1/10000 - 1/50000. Detects a band of approximately 85 kDa (predicted
molecular weight: 81 kDa).
ICC/IF
1/250 - 1/500.
Flow Cyt
1/50 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an
isotype control with this antibody.
IP
1/50.
Target
Function
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Pathway
Glycan biosynthesis; glycogen biosynthesis.
Involvement in disease
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b)
[MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic
disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role
of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle
work.
Sequence similarities
Belongs to the glycosyltransferase 3 family.
Anti-Glycogen synthase 1 antibody [EP817Y] images
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Overlay histogram showing HEK293 cells
stained with ab40810 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions followed by the
Flow Cytometry - Anti-Glycogen synthase 1
antibody (ab40810, 1/1000 dilution) for 30
antibody [EP817Y] (ab40810)
min at 22°C. The secondary antibody used
was DyLight® 488 goat anti-rabbit IgG (H+L)
(ab96899) at 1/500 dilution for 30 min at
22°C. Isotype control antibody (black line)
was rabbit IgG (monoclonal) (0.1μg/1x106
cells) used under the same conditions.
Unlabelled sample (blue line) was also used
as a control. Acquisition of >5,000 events
were collected using a 20mW Argon ion laser
(488nm) and 525/30 bandpass filter. This
antibody gave a positive signal in HEK293
cells fixed with 80% methanol (5
min)/permeabilized with 0.1% PBS-Tween for
20 min used under the same conditions.
Anti-Glycogen synthase 1 antibody [EP817Y]
(ab40810) at 1/50000 dilution + HeLa cell
lysate at 10 µg
Predicted band size : 81 kDa
Observed band size : 85 kDa
Western blot - Glycogen synthase 1 antibody
[EP817Y] (ab40810)
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ab40810 at a 1:250 dilution staining Hela
cells using anti-Glycogen Synthase 1
RabMAb.
Immunocytochemistry/ Immunofluorescence Glycogen synthase 1 antibody [EP817Y]
(ab40810)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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