Product datasheet
Anti-Lactate Dehydrogenase antibody (Biotin) ab7638
1 Abreviews 3 References
Overview
Product name
Anti-Lactate Dehydrogenase antibody (Biotin)
Description
Goat polyclonal to Lactate Dehydrogenase (Biotin)
Conjugation
Biotin
Tested applications
ELISA, Immunomicroscopy, WB, Dot Blot
Species reactivity
Reacts with: Mouse, Rabbit, Human, Chinese Hamster
Predicted to work with: a wide range of other species
Immunogen
Full length native Lactate Dehydrogenase (purified).
Positive control
purified enzyme from rabbit muscle(Worthington Biochemicals)
General notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC
molecules per goat IgG molecule.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Purity
IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step
process which includes delipidation, salt fractionation and ion exchange chromatography
followed by extensive dialysis against the buffer stated above.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab7638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
1
Application
Abreviews
Notes
Immunomicroscopy
WB
Dot Blot
Application notes
Dot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Please note: The non-conjugated version of this
antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on
the datasheet for ab2101).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
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