Biology 6 Schedule – Spring 2016 Week Date Lecture Topic Chap. Laboratory Exercises T 1/26 Introduction to Microbiology 1 Introduction to the Lab Ex. 2-1: Ubiquity of Microorganisms Th 1/28 Chemistry 2 Ex. 1-3: Aseptic Transfer and Inoculation Ex. 1-4: Streak Plate Method of Isolation T 2/2 Prokaryotic Cells 4 Ex. 2-2: Colony Morphology Ex. 2-3: Slant Morphology Ex. 2-4: Broth Morphology Th 2/4 Eukaryotic Cells 4 Observe Tuesday’s Cultures T 2/9 Metabolism 5 Ex. 2-7: Fluid Thioglycollate Medium Ex. 7-3: Antimicrobial Susceptibility *Disinfectants Th 2/11 Metabolism 5 Observe Tuesday’s Cultures T 2/16 Test 1 Growth and Culturing 6 *Ex. 2-9: Temperature Ex. 2-11: Osmotic Pressure Th 2/18 Growth and Culturing 6 Observe Tuesday’s Cultures Ex. 3-5: Smear Preparation T 2/23 Molecular Biology 8 Ex. 3-1: Introduction to the Microscope Ex. 3-5: Simple Stains Th 2/25 Molecular Biology 8 Ex. 3-7: Gram Stain T 3/1 Molecular Biology 8 Ex. 3-8: Acid Fast Stain Ex. 3-10: Endospore Stain Th 3/3 Biotechnology 9 Lab Exam #1 (Through Week 5) T 3/8 Test 2 Classification 10 Th 3/10 Viruses 13 Notes 1 2 3 4 5 6 7 T 3/15 Prokaryotes 11 Th 3/17 Eukaryotes 12 8 Ex. 4-4: Mannitol Salts Agar Ex. 4-5: MacConkey Agar Ex. 5-3: Phenol Red Broth Observe Tuesday’s Cultures Ex. 5-6: Catalase Test Ex. 5-7: Oxidase Test Ex. 5-15: Lipid Hydrolysis Ex. 5-16: Casein Hydrolysis Ex. 5-17: Gelatin Hydrolysis Observe Tuesday’s Cultures Ex. 5-4: MR-VP Ex. 5-8: Nitrate Reduction See Text Ch. 6 *See Lab Notes for protocol *See Lab Notes for protocol T 3/22 Eukaryotes 12 Ex. 5-9: Citrase Test Ex. 5-18: Urea Hydrolysis Ex. 5-20: SIM Medium Th 3/24 Antimicrobial Agents 7, 20 Observe Tuesday’s Cultures 9 T 3/29 Spring Break Th 3/31 T 4/5 Pathology and Epidemiology Th 4/7 Pathogenicity 14 10 15 Staphylococcus ID: Ex. 4-4: Mannitol Salt Agar Ex. 5-14: DNA Hydrolysis Ex. 5-27: Coagulase Test Ex. 5-24: Novobiocin Test Lab Exam #2 (Weeks 6-9) Observe Tuesday’s Cultures Streptoccocus ID: Ex. 4-3: Bile Esculin Test Ex. 5-24: Bacitracin Test Ex. 5-25: Blood Agar Plate * NaCl Broth Test 3 (Weeks 7-9) Innate Immunity 16 Th 4/14 Innate Immunity 16 Observe Tuesday’s Cultures *Dichotomous Key Exercise *See Lab Notes for protocol T 4/19 Adaptive Immunity 17 *Culture Identification Exercise *See Lab Notes for protocol Th 4/21 Applications of Immunity 18 Culture Identification Exercise T 4/26 Test 4 (Weeks 10-12) HIV and AIDS 19 Th 4/28 HIV and AIDS 19 Ex. 5-30: Enterotube Test Culture Identification Exercise T 4/12 11 *See Lab Notes for protocol 12 Culture Identification Exercise 13 T 5/3 Diseases of the Skin 21 *Staphylococcus Quick Agglutination Test *Streptococcus Quick ELISA Test Culture Identification Exercise Th 5/5 Diseases of the Nervous, Circulatory and Lymphatic Systems 22, 23 Ex. 11-6: ELISA Culture Identification Exercise T 5/10 Diseases of the Respiratory and Digestive Systems 24, 25 Review and Lab Cleanup Culture Identification Reports Due Th 5/12 Diseases of the Reproductive System Extra Credit Due 26 Lab Final Exam (Comprehensive) Th 5/19 Test 5 – 4:00pm 14 15 Final *See Lab Notes for protocol