Human Physiology Laboratory
BIO 5, Spring 2016
Denise Lim, Instructor
Aptos Office: Room 621, 479-6339, delim@cabrillo.edu
Purpose
The purpose of the laboratory portion of this course is to give you a hands-on demonstration of the
human physiological processes we discuss in lecture. You will measure the ways in which the body
responds to varying stimuli and observe how those responses contribute to the maintenance of
homeostasis. The labs are not only designed to support physiological concepts learned in lecture, but to
demonstrate the process of scientific investigation as well. We firmly believe the journey is just as
important as the destination and experimental methodology will be emphasized.
Learning Outcomes: By the end of this lab class there are a series of skills that you will have acquired.
You should be able to:
1. Describe with detail the functioning of specific body systems at both the cellular level and at
the system level to predict a response to changes in homeostasis.
2. Apply your understanding of the individual systems to interactions between multiple systems.
Lab Objectives:
1. Collect data from human subjects with a data acquisition program
2. Interpret laboratory results and draw scientific conclusions from those results
3. Contribute constructively to group discussions
Required Materials
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Human Physiology, BIO 5 Laboratory Manual. When you are notified, it will be available at the
Print Smith in Aptos Village (8047 Soquel Drive, 831-688-1538).
Pre-Lab Questions
– downloaded from the BIO 5 Lab website (http://www.cabrillo.edu/~dlim/bio5/bio5labs.html):
Graph paper
Calculator – bring one to each class meeting. You will need them for quizzes. You will not be
allowed to use your phone.
Supplies for the Spirometry Lab: Mouthpiece, filter, and a nose clip. These supplies are available at
the Cabrillo College Aptos Bookstore (about $10). Only one set of spirometry supplies are needed
for each group.
BIO 5 Lab Syllabus, Lim
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Pre-Lab Questions
These questions are based on the exercise we will doing that day and will require you to read the
exercise and any background material that is relevant. The single most important thing you can do to
ensure you will be as successful as possible is READ THE LAB EXERCISE BEFORE YOU COME
TO CLASS. This will help you understand the objectives of the day’s exercise and familiarize you with
protocols. These questions are due at the beginning of each lab class and will not be accepted late, unless
notified otherwise. If you miss a lab, the Pre-Lab questions cannot be turned in at a later date. One PreLab assignment will automatically be dropped in calculating your final grade (in other words, you get
one freebie).
Lab Exercises
You will work in groups of three or four students per workstation. The lab exercises include the use of a
computer-interfaced physiology data acquisition unit called BIOPAC, a variety of in vitro techniques to
study physiological phenomena, and computer simulations called PhysioEx. The Laboratory Manual can
be purchased from the Print Smith.
Follow Up Questions: These questions are found scattered throughout the Laboratory Manual,
particularly at the end of each exercise, and can be downloaded from the BIO 5 website for PhysioEx
exercises. They are meant to help you prepare for the weekly quizzes. If you discuss and answer these
questions with your lab partners before leaving class each week, you will do well on the quizzes.
PhysioEx: Computer Simulation Experiments
Simulations are used primarily because they allow us to perform experiments that may be too complex
or costly to perform in our lab environment. You are required to complete the entire assigned exercise in
class with your lab partners. The data you collect while performing the simulation will be recorded and
reviewed for the lab quizzes. Lab time will be spent collecting data and discussing the results and
Follow-Up Questions. You are expected to answer both the worksheet questions and the review
questions as a group. You will be quizzed on each PhysioEx exercise the following week, using the
same format as for other lab exercises.
Lab Quizzes
The week following each in-class lab exercise, you will be quizzed on your understanding of that
activity. Quizzes will consist of 10 short answer essay questions concerning physiological concepts,
experimental protocols, data analysis, and conclusions or explanations of the results of the experiments
you performed. This includes the underlying physiological processes or mechanisms involved, and any
experimental techniques, equipment, and reagents (chemicals) involved.
How to Study for a Lab Quizzes
1. Review physiological concepts
2. Review lab protocols – understand how a protocol contributed to obtaining the results
3. Review results – know what happened and why it happened
4. Review Questions from the lab exercises and the Pre-Lab assignment – this is probably the most
important part of preparing for the quizzes. Practice writing clear, concise answers to these
questions before you take the quiz.
You will be expected to understand the methods used to collect the results as well as the
physiological significance of the results themselves. Questions concerning data analysis will involve
interpretation, calculation and/or graphing, as well as explanation of unexpected results, depending on
the exercise. Please keep all graded Quizzes and that are returned until the semester is over. If
BIO 5 Lab Syllabus, Lim
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there is any question regarding a score, you will be required to provide the original quiz for
reconsideration.
If you miss a quiz because of an absence, no matter the reason, you have a maximum of two
weeks to make it up. You will lose 1 point for each week late. If it is not made up within two weeks, you
will get a zero for that quiz. It is your responsibility to request a make-up from me. Your lowest quiz
grade will be dropped at the end of the semester.
Poster Presentations
This is a group research project on a disease chosen by your group and approved by me. Your research
will be presented in poster format and presented to the class, much like the way scientific research is
presented at conferences. You will receive more detailed information and a grading rubric at a later date.
Calculating Your Grade
The final lab grade will then be combined with your lecture scores and weighted as 30% of your final
grade.
LAB GRADE BREAKDOWN
Your Points
Possible
Points
Pre-Lab Questions (12 X 5 pt. each)
60
Quizzes (approximately 10 quizzes X 10 pt. each)
100
Concept Map Take Home Assignment
15
Poster Presentations
50
Total
225
Average Lab Percent = Your Pts/Total Pts.
The total possible points may change depending the number of quizzes actually given. The points listed
above are an estimate.
Use the following formulae to calculate your total grade.
For the 70/30 grading option:
(Average Lecture Percentage X 0.7) + (Average Lab Percentage X 0.3)
BIO 5 Lab Syllabus, Lim
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Lab Schedule for Lim
Human Physiology, BIO 5
Spring, 2016
*Please wear athletic shoes for these exercises. You will be expected to participate in an aerobic step test
to evaluate your aerobic fitness unless you have health considerations.
**Purchase spirometry supplies: bio filter, mouthpiece, nose clip
DATE
LABORATORY
EXERCISES
January 27, 29
*Exercise 1: Aerobic Capacity
February 3, 5
Exercise 2: Acids and Buffers
WEEK
1
2
3
4
5
6
7
8
9
February 10, 12
Concept Mapping Take Home Assignment
February 17, 19
Exercise 3: Movement Across
Cell Membranes
Exercise 4: PhysioEx #4 (Activities 1-4)
The Endocrine System
Exercise 5: PhysioEx #3
Action Potentials
*Exercise 6:
Biopac Tutorial
Exercise 7:
Muscle Physiology I – Electromyography
Exercise 8:
Muscle Physiology II – Twitch and Summation
February 25, 27
March 2, 4
March 9, 11
March 16, 18
March 23, 25
Mar 30, April 1
10
11
12
13
14
15
April 6, 8
April 13, 15
April 20, 22
April 27, 29
May 4, 6
May 11, 13
BIO 5 Lab Syllabus, Lim
SPRING BREAK
*Exercise 9:
The Cardiac Cycle – Electrocardiography
Exercise 10: PhysioEx #5
Fluid Dynamics
Exercise 11: Blood Typing
**Exercise 12:
Spirometry & Pulmonary Function
Exercise 13: PhysioEx #9
Renal Physiology
Poster Presentations
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