Product datasheet
Anti-AGBL5 antibody ab118621
2 Images
Overview
Product name
Anti-AGBL5 antibody
Description
Rabbit polyclonal to AGBL5
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Recombinant fragment, corresponding to amino acids 150-396 of Human AGBL5, isoform 2
(BC007415).
Positive control
HeLa, 786 O and Human fetal brain cell lysates; Human fetal brain tissue.
Properties
Form
Lyophilised:Reconstitute in 200ul sterile water.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Purification notes
ab118621 is purified by a protein affinity column.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab118621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/1000. Detects a band of approximately 80, 90 kDa (predicted
molecular weight: 90 kDa).
IHC-P
1/100 - 1/500.
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Target
Function
Metallocarboxypeptidase that mediates tubulin deglutamylation. Specifically catalyzes the
deglutamylation of the branching point glutamate side chains generated by post-translational
glutamylation in proteins such as tubulins. In contrast, it is not able to act as a long-chain
deglutamylase that shortens long polyglutamate chains, a process catalyzed by AGTPBP1/
CCP1, AGBL1/CCP4 and AGBL4/CCP6.
Tissue specificity
Expressed in brain.
Sequence similarities
Belongs to the peptidase M14 family.
Cellular localization
Cytoplasm > cytosol. Nucleus. Mainly cytoplasmic. Slight accumulation in the nucleus is
observed.
Anti-AGBL5 antibody images
All lanes : Anti-AGBL5 antibody (ab118621)
at 1/500 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 786 O cell lysate
Lane 3 : Human fetal brain lysate
Western blot - Anti-AGBL5 antibody (ab118621)
Predicted band size : 90 kDa
Observed band size : 80,90 kDa
ab118621, at a 1/100 dilution, staining
AGBL5 in the cytoplasm and nucleus of
formalin fixed, paraffin embedded Human
fetal brain tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-AGBL5
antibody (ab118621)
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