Product datasheet
Anti-Glucose Transporter GLUT1 antibody [EPR3915]
(HRP) ab195021
2 Images
Overview
Product name
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (HRP)
Description
Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1 (HRP)
Conjugation
HRP
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human Glucose Transporter GLUT1 aa 450 to the C-terminus.
Positive control
WB: Mouse brain and Human fetal brain tissue lysates. IHC-P: normal human colon tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
Anti-Glucose Transporter GLUT1 antibody (Alexa Fluor® 488) [EPR3915] (ab195359)
Anti-Glucose Transporter GLUT1 antibody (Alexa Fluor® 594) [EPR3915] (ab206360)
Anti-Glucose Transporter GLUT1 antibody (Alexa Fluor® 647) [EPR3915] (ab195020)
Anti-Glucose Transporter GLUT1 antibody (Phycoerythrin) [EPR3915] (ab209449)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)
KD = 7.70 x 10 -12 M
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Learn more about KD
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Immunogen affinity purified
Clonality
Monoclonal
Clone number
EPR3915
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab195021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
WB
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 54
kDa).
Target
Function
Facilitative glucose transporter. This isoform may be responsible for constitutive or basal
glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses
including both pentoses and hexoses.
Tissue specificity
Expressed at variable levels in many human tissues.
Involvement in disease
Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome
(GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This
disease causes a defect in glucose transport across the blood-brain barrier. It is characterized
by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an
exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of
sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is
characterized by attacks of involuntary movements triggered by certain stimuli such as sudden
movement or prolonged exercise. In some patients involuntary exertion-induced dystonic,
choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia.
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose
transporter subfamily.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by
mass spectrometry in melanosome fractions from stage I to stage IV.
Anti-Glucose Transporter GLUT1 antibody [EPR3915] (HRP) images
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IHC image of Glucose Transporter GLUT1
staining in a section of formalin-fixed paraffinembedded normal human colon tissue*,
performed on a Leica BOND. The section
was pre-treated using heat mediated antigen
retrieval with sodium citrate buffer (pH6,
epitope retrieval solution 1) for 20mins. The
section was then incubated with ab195021 at
1/100 dilution, for 15 mins at room
temperature. DAB was used as the
chromogen. The section was then
Immunohistochemistry (Formalin/PFA-fixed
counterstained with haematoxylin and
paraffin-embedded sections) - Anti-Glucose
mounted with DPX. The inset negative control
Transporter GLUT1 antibody [EPR3915] (HRP)
image is taken from an identical assay
(ab195021)
without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
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All lanes : Anti-Glucose Transporter GLUT1
antibody [EPR3915] (HRP) (ab195021) at
1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Human) Tissue Lysate - fetal
normal tissue
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Western blot - Anti-Glucose Transporter GLUT1
antibody [EPR3915] (HRP) (ab195021)
Performed under reducing conditions.
Predicted band size : 54 kDa
Observed band size : 42 kDa
Exposure time : 30 seconds
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 3% milk before being incubated with
ab195021 overnight at 4°C. Antibody binding
was visualised using ECL development
solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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