Overview
Product name
Description
Conjugation
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-GAPDH antibody [EPR16891] (Phycoerythrin)
Rabbit monoclonal [EPR16891] to GAPDH (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
Flow Cyt, ICC/IF
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Fish, Monkey, Zebrafish, Xenopus tropicalis
Recombinant fragment within Mouse GAPDH aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P16858
Flow Cyt: HeLa cells ICC/IF: HeLa cells
This product is a recombinant rabbit monoclonal antibody.
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-GAPDH antibody [EPR16891] ( ab181602 )
Anti-GAPDH antibody [EPR16891] (Alexa Fluor 488) ( ab201768 )
Anti-GAPDH antibody [EPR16891] (Alexa Fluor 647) ( ab201272 )
Anti-GAPDH antibody [EPR16891] (HRP) ( ab201822 )
Anti-GAPDH antibody (Alexa Fluor 594) [EPR16891] ( ab206371 )
Properties
Form
Storage instructions
Storage buffer
Liquid
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at +4°C.
Do Not Freeze. Store In the Dark.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
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Purity
Clonality
Clone number
Isotype
Protein A purified
Monoclonal
EPR16891
IgG
Applications
Our Abpromise guarantee covers the use of ab208877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
Flow Cyt 1/2500.
ICC/IF ab209478 -Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody.
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)
Target
Function
Pathway
Sequence similarities
Post-translational modifications
Cellular localization
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as
SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting Dglyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated.
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Anti-GAPDH antibody [EPR16891] (Phycoerythrin) images
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Flow Cytometry - Anti-GAPDH antibody
[EPR16891] (Phycoerythrin) (ab208877)
Overlay histogram showing HeLa cells stained with ab208877 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-
20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody
(ab208877, 1/2500 dilution) for 30 min at
22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin
( ab209478 ) used at the same concentration and conditions as the primary antibody.
Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser
(488nm) and 585/40 bandpass filter.
Immunocytochemistry/ Immunofluorescence -
Anti-GAPDH antibody [EPR16891]
(Phycoerythrin) (ab208877) ab208877 staining GAPDH in HeLa cells.
The cells were fixed with 100% methanol
(5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1%
BSA/10% normal goat serum/0.3M glycine in
0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208877 at
1/100 dilution (Pseudocolored in green) and ab195884 , Rat monoclonal to Tubulin (Alexa
Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope
(Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
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Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
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