Product datasheet
Anti-Aldolase antibody [EPR9724(B)] ab169544
3 Abreviews 1 References 3 Images
Overview
Product name
Anti-Aldolase antibody [EPR9724(B)]
Description
Rabbit monoclonal [EPR9724(B)] to Aldolase
Tested applications
WB, IHC-P, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide corresponding to residues in Human Aldolase (UniProt P04075).
Positive control
A549, fetal liver, HeLa, fetal brain and Human skeletal muscle lysates; Human muscle and
ovarian carcinoma tissues.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Aldolase antibody (HRP) [EPR9724(B)] (ab200774)
Anti-Aldolase antibody (Alexa Fluor® 488) [EPR9724(B)] (ab200771)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR9724(B)
Isotype
IgG
Applications
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Our Abpromise guarantee covers the use of ab169544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 39 kDa.
IHC-P
1/100 - 1/250.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IP.
Target
Function
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding
protein.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate
from D-glucose: step 4/4.
Involvement in disease
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881];
also known as red cell aldolase deficiency. A metabolic disorder associated with increased
hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and
rhabdomyolysis.
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family.
Anti-Aldolase antibody [EPR9724(B)] images
Immunohistochemical analysis of paraffinembedded Human ovarian carcinoma tissue
labeling Aldolase with ab169544 at 1/100
dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Aldolase
antibody [EPR9724(B)] (ab169544)
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All lanes : Anti-Aldolase antibody
[EPR9724(B)] (ab169544) at 1/1000 dilution
Lane 1 : A549 lysate
Lane 2 : Fetal liver lysate
Lane 3 : HeLa lysate
Lane 4 : Fetal brain lysate
Lane 5 : Human skeletal muscle lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-Aldolase antibody
Secondary
[EPR9724(B)] (ab169544)
HRP labeled goat anti-rabbit at 1/2000
dilution
Predicted band size : 39 kDa
Immunohistochemical analysis of paraffinembedded Human muscle tissue labeling
Aldolase with ab169544 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Aldolase
antibody [EPR9724(B)] (ab169544)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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