Product datasheet Anti-Aldolase antibody [EPR9724(B)] ab169544 3 Abreviews 1 References 3 Images Overview Product name Anti-Aldolase antibody [EPR9724(B)] Description Rabbit monoclonal [EPR9724(B)] to Aldolase Tested applications WB, IHC-P, ICC/IF Species reactivity Reacts with: Mouse, Rat, Human, Pig Immunogen Synthetic peptide corresponding to residues in Human Aldolase (UniProt P04075). Positive control A549, fetal liver, HeLa, fetal brain and Human skeletal muscle lysates; Human muscle and ovarian carcinoma tissues. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Alternative versions available: Anti-Aldolase antibody (HRP) [EPR9724(B)] (ab200774) Anti-Aldolase antibody (Alexa Fluor® 488) [EPR9724(B)] (ab200771) Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR9724(B) Isotype IgG Applications 1 Our Abpromise guarantee covers the use of ab169544 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes WB 1/1000 - 1/10000. Predicted molecular weight: 39 kDa. IHC-P 1/100 - 1/250. ICC/IF 1/50 - 1/100. Application notes Is unsuitable for Flow Cyt or IP. Target Function Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein. Pathway Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. Involvement in disease Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis. Sequence similarities Belongs to the class I fructose-bisphosphate aldolase family. Anti-Aldolase antibody [EPR9724(B)] images Immunohistochemical analysis of paraffinembedded Human ovarian carcinoma tissue labeling Aldolase with ab169544 at 1/100 dilution. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR9724(B)] (ab169544) 2 All lanes : Anti-Aldolase antibody [EPR9724(B)] (ab169544) at 1/1000 dilution Lane 1 : A549 lysate Lane 2 : Fetal liver lysate Lane 3 : HeLa lysate Lane 4 : Fetal brain lysate Lane 5 : Human skeletal muscle lysate Lysates/proteins at 10 µg per lane. Western blot - Anti-Aldolase antibody Secondary [EPR9724(B)] (ab169544) HRP labeled goat anti-rabbit at 1/2000 dilution Predicted band size : 39 kDa Immunohistochemical analysis of paraffinembedded Human muscle tissue labeling Aldolase with ab169544 at 1/100 dilution. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase antibody [EPR9724(B)] (ab169544) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3 4