Overview
Product name
Description
Conjugation
Tested applications
Species reactivity
Immunogen
Positive control
Anti-Aldolase antibody (HRP)
Goat polyclonal to Aldolase (HRP)
HRP
WB, Dot Blot, ELISA, Electron Microscopy
Reacts with: Rabbit, Human
Full length Aldolase protein (Rabbit muscle).
WB:Human A293, MCF7, NMB231 and HBL 100 cell lysates.
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Isotype
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
IgG fraction
This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab34598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
Dot Blot
ELISA
Electron Microscopy
Application notes Dot: Use at an assay dependent dilution.
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Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
WB: 1/1000. Predicted molecular weight: 39 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Pathway
Involvement in disease
Sequence similarities
Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
Belongs to the class I fructose-bisphosphate aldolase family.
Anti-Aldolase antibody (HRP) images
Anti-Aldolase antibody (HRP) (ab34598) at
1/1000 dilution + A293 cell lysate
Predicted band size : 39 kDa
Observed band size : 41 kDa
Western blot - Aldolase antibody (HRP) (ab34598)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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