Anti-Aldolase antibody (HRP) ab34598 Product datasheet 1 Image Overview

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Overview

Product name

Description

Conjugation

Tested applications

Species reactivity

Immunogen

Positive control

Anti-Aldolase antibody (HRP)

Goat polyclonal to Aldolase (HRP)

HRP

WB, Dot Blot, ELISA, Electron Microscopy

Reacts with:

Rabbit, Human

Full length Aldolase protein (Rabbit muscle).

WB:Human A293, MCF7, NMB231 and HBL 100 cell lysates.

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Isotype

Liquid

Shipped at 4°C. Store at +4°C.

Preservative: 0.01% Gentamicin Sulfate

Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2

IgG fraction

This antibody was purified by a multi step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.

Polyclonal

IgG

Applications

Our Abpromise guarantee covers the use of

ab34598

in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes

WB

Dot Blot

ELISA

Electron Microscopy

Application notes

Dot: Use at an assay dependent dilution.

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Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.

ELISA: 1/1000 - 1/2000.

WB: 1/1000. Predicted molecular weight: 39 kDa.

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Pathway

Involvement in disease

Sequence similarities

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.

Belongs to the class I fructose-bisphosphate aldolase family.

Anti-Aldolase antibody (HRP) images

Anti-Aldolase antibody (HRP) (ab34598) at

1/1000 dilution + A293 cell lysate

Predicted band size :

39 kDa

Observed band size :

41 kDa

Western blot - Aldolase antibody (HRP) (ab34598)

Please note:

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Our Abpromise to you: Quality guaranteed and expert technical support

Replacement or refund for products not performing as stated on the datasheet

Valid for 12 months from date of delivery

Response to your inquiry within 24 hours

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We investigate all quality concerns to ensure our products perform to the highest standards

If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.

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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors

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