Biology 612 Freshwater Biology Fall 2012 Exam 1 Name______________________ 1. Why do both human population growth and increasing affluence (standard of living) mean that freshwater resources will come under greater stress in the future? (5) 2. Describe how a swimming bacterium experiences swimming in water relative to a large fish with respect to turbulence, streamlining, the effect of a wall, viscosity, and inertia (5) 3. Describe 3 major ways that lakes form, and what you expect their relative age to be for each cause of formation (6) 4. Fill in the following table on properties of water (10) Property Aspect Causes water to assume a crystal shape upon freezing and many other water properties Temperature of maximum density Causes water to creep up fine tube Viscosity as temperature increases Relative amount of salt dissolved as temperature increases Relative amount of gas dissolved as temperature increases Density of solid phase relative to liquid phase Relative density as dissolved salt increases buffers water against rapid changes in temperature Effect of 5 degree C increase from 5 to 10 relative to that from 20 to 25 degrees C 5. How do the physical characteristics of a reservoir vary from that of most natural lakes? List 5 aspects. (5) 6. Josh Perkin talked about how the dewatering of streams can cause fragmentation of stream networks. What life history reproductive tactic of some fish species (e.g. the plains minnow) is particularly sensitive to this dewatering? (5) 7. What did James Whitney talk about (in a couple sentences) with respect to the Colorado River fish communities (5)? 8. Wetlands were formerly thought to be useless, but we now know wetlands provide some essential ecosystem goods and services to people. List 4 of those services (4). 9. What does the urbanization of watersheds do to peak discharge of floods and how would this influence: (a) erosion rates, (b) the average size of particle that moves downstream, and (c) the rate of meander formation ? (6) 10. Why is the biological species definition not useful many of the species we study as aquatic ecologists (3)? 1 11. Why are ribosomal RNA (rRNA) sequences useful taxonomic tools for determining large differences between organisms (i.e. long periods of evolutionary separation), as well as differences between closely related species? (5) 12. Match the terms with the letters (20) Dimictic Hypolimnion Epilithic Archaea Exploitation Mutualism Emergent A. B. C. D. E. F. G. Chemoautotrophic Heterotrophic H. I. Seiche Langmuir circulation J. K. Fetch L. Monomictic M. 2nd order stream Thalweg Everglades Karst N. O. P. Q. Blue light Water table R. S. Light attenuation T. forms when 2 first order streams join mixing twice a year fastest flowing portion of lake or river self feeding using chemicals causes streaks on lakes mixing once a year managers trying to save by diverting water flow back into this large wetland +/+ interaction between species rugged landscape formed by limestone dissolution growing on rocks transmitted more readily in pure water than red light must get carbon from organic carbon sources Where aquifer meets vadose zone or surface bottom of a stratified lake Growing out of the water one of the three domains of life generally logarithmic loss with depth in lake rocking of water in lake Distance across which water acts on a lake +/- interaction between two species 13. Fill in the blanks in the sentences (10). Molecular diffusion is slower than _____________ diffusion. Mixing causes this type of diffusion, but such mixing does not occur in the ___________ zone very close to solid surfaces. As geometric objects get larger their surface __________ to ____________ ratios get smaller, leading to diffusion limitations. Larger multicellular organisms cannot move materials into their cells quickly enough with molecular diffusion so they have _____________ systems that help overcome diffusion limitation. Other factors that influence diffusion rates include temperature (with _________ rates of diffusion at higher temperatures), concentration ( 2 with ____________ rates of diffusion with greater differences in concentration) and distance ( with _____________ rates of diffusion with greater distances between two points). Fine sediments have lower diffusion rates of materials through them than coarser gravel because __________ number is smaller, with _____________ viscosity because of small distances between particles. 14. Fill in more blanks (11). Cyanobacteria are bacteria, not _____________. They grow in nutrient rich water and are problematic because they can be ________________ and also can make water unfit to drink because of ____________ and odor problems. They can synthesize at least two forms of toxins, ____________ and ____________. They do well in low light because they have special pigments that absorb in the green region, unlike eukaryotic algae that mainly use _______________ for light absorption. They are also successful because they synthesize gas vesicles that make them __________ dense than water and allow them to float. These floating scums are not pleasant and can ___________ property values of lake-front properties. They are unlike diatoms, in part, because they do not have a cell wall made of _____________. The cell wall of diatoms is used in forensics to help confirm victims of _______________. If cells of either of these groups are found on the bottom, they are referred to as benthic, if they are suspended they are referred to as ________________. 3 Freshwater Ecology Biol 612 2nd Midterm Fall 2012 ____________________Name 1. Draw the nitrogen cycle as presented in class and in the book (20) 2. Draw the carbon cycle as presented in class and in the book (20) \ 3. Match letter on right with term on left (20) Trichoptera Epemeroptera Plecoptera Sponge Unionid mussels Daphnia Copepoda Zebra mussel alpha diversity α beta diversity β eveness redox Amphipoda Isopoda Geologically old lakes large area versus small area habitat sulfate conductivity pH higher altitude A. Cladocera B. dorsal-ventrally flattened crustacean C. Mostly marine group with silica spicules but some species in fresh waters D. juvenile naupliar stages E. the relative number of free electrons in a solution F. laterally flattened crustacean G. more diversity expected H. stone fly I. can serve as an electron acceptor for anaerobic respiration J. diversity among habitats K. Caddis fly L. Introduced to great lakes in ship ballast M. saturation concentration of dissolved oxygen lower N. Endangered group, formerly used to make buttons O. a measure of the degree of equal distribution of numbers of each species in a community P. a measure of the number of dissolved ions in solution Q. negative log to base 10 of hydrogen ion concentration R. High degree of endemism S. within habitat diversity T. Mayfly 4 4. Amphibians are threatened by pollution and several other factors that are leading to their extinction around the globe. What are some of the other factors leading to the loss of species and what is it about amphibian life cycles that make them particularly susceptible to pollutants? (4) 5. Describe how nucleic acids (DNA and RNA), lipids, and proteins are altered in organisms that can withstand very high temperatures (6) 6. Why do depths of saline lakes typically fluctuate widely over years? (4) 7. Humans are causing the 6th great extinction on Earth, with probably half the species disappearing in your life time. Why does diversity matter to humanity? (5) 8. If you dumped a whole bunch of iron into a lake, what would you expect to happen to the concentrations of phosphate and sulfate? (4) 9. Why are deep groundwaters harsh habitats? (4) 10. What caused the disaster at Lake Nyos? (4) 11. Describe the difference between net and gross primary production and how they are related to respiration? You can do this verbally or with an equation (5) 12. Give the equations for photosynthesis and respiration (4) 5 Biol 612 Freshwater Ecology 3rd Midterm 2012 Name___________________ 1. Match the terms (20) Term Chronic Match A. Increase in concentration from environment into a single organism B. Greater aluminum solubility C. Growth as a function of external nutrient concentration D. Growth as a function of intracellular nutrient concentration E. 106:16:1 C:N:P F. More nutrient rich than mesotrophic G. Following magnetic lines to find sediment H. Using organism remains in sediments of lakes to infer past environmental conditions I. CH2O + O2 → CO2 + H2O+ chemical energy J. Using organisms present to indicate state of environment K. Uptake as a function of external concentration L. Search strategy M. Pulsed exposure N. Can be used as food web tracer O. Hormone mimics that are active at low concentration and influence development P. Macrophyte removal Q. Long term R. Can cause decreased diversity in urban areas S. CO2 + H2O + light → CH2O + O2 T. Increase of concentration through the food web Acute Bioconcentration Biomagnification Bioassessment Ecoestrogens Paleolimnology Increased fish mortality with lower pH Road salt Monod equation Michaelis-Menten equation Droop equation Redfield ratio Hypertrophic Grass carp Photosynthesis Respiration Random walk Magnetotaxis Stable isotopes of C and N 2. Suspended solids can be thought of as a pollutant, but they are a natural part of many ecosystems. Under what conditions do you think sediments should be defined as a pollutant, and what types of particulate materials might be beneficial to river and stream organisms? (5) 6 12 10 8 6 4 2 0 ingestion rate Filtering rate 3. What are the EC50 and LD50 , and which one is generally lower and why? (5) 4. Why is nutrient concentration not necessarily the best index of nutrient availability to satisfy demand? (5) 5. Why was eutrophication reversible in Lake Washington, but not in Lake Trummen? (5) 6. List 5 ways that aquatic prey have evolved to avoid predation, and potential non-lethal implications (evolutionary costs) of each (5) 7. Why is a “two story” fishery threatened by eutrophication? (5) 8. Why might the trophic cascade not apply to the microbial loop? (5) 9. We have a stream that has completely dried, and now just started flowing. What types of organisms might show up first and what might end up there last over the next six months? What life history characteristics are mostly likely to be associated with the first versus the last organisms (6)? 10. Describe the seasonal succession in a temperate oligotrophic lake with respect to phosphorus, silica, diatoms, cyanobacteria, phytoplankton, zooplankton, and larval fish. You can use a series of graphs or words (7) 11. Fill in the following graphs for Daphnia feeding on different concentrations of algae (one line each) (6) 0 0.5 1 15 10 5 0 0 1.5 0.5 1 1.5 Algal cells per uL Algal cells per uL 12. Fill in the blanks: Species_____________ will win at high SiO4 and low PO4 species, and Species ______ will win at low SiO4 and low PO4 and they will ____________ _______ (6) 7 13. Give some reasons why top down control of eutrophication might fail to lower algae in the in a short term and long term management plan (5). 14. List 5 potential negative consequences of non-native fish species introductions (5) 15. List 3 problems that are endangering the Everglades in Florida (3) 8 Biology 612 Final Fall 2012 name_________________________ 1. Describe how a reservoir might disrupt the natural sequence of a river continuum (5) 2. Indicate , high (H) medium (M) or low (L) , how the habitats rank on the following abiotic and biotic scales. See first line for example (5) Feature Deep groundwater Light L Hydrologic turnover time Seasonal variance Allochthonous C inputs Terrestrial input Importance photosynthesis Lake H Small Stream M 3. Describe the concept of nutrient spiraling and how temperature and water velocity might be related to spiral length (5) 4. Match the material (25) Item Phytoplankton zooplankton benthic Related A. On the bottom B. Macrophyte removal C. Effect of top predator on primary producer D. Without dissolved oxygen E. Low Reynolds number F. One of three domains of life G. Suspended producers H. Between habitats I. Mineralization/ remineralization J. Area above groundwater K. Grabben and horst L. Carbon flux through microbial component of food web M. More intense runoff N. Suspended animals O. Increase in toxin moving up the food web P. Ephemeroptera Q. Silicon cell wall (frustule) R. Both species benefit S. Organism colonization in new habitat mutualism photoinhibition Cyanobacteria Mayfly Caddisfly Nutrient supply source Diatom Tectonic lake Net primary production Biomagnification Redfield ratio Asian carp Primary succession Trophic cascade Microbial loop Anoxic 9 Light attenuation High viscosity Beta diversity Vadose zone Urbanization Archaea T. U. V. W. X. Y. Logarithmic with depth High light Trichoptera Gross primary production - respiration 106:16:1 C:N:P Nitrogen fixing heterocysts 5. Diagram and label the nitrogen cycle as it was done in class (10) 6. Describe the seasonal pattern of lake stratification in a temperate deep lake, and what causes it to form and mix and when (5) 7. The trophic cascade has been linked to deeper epilimnion because it clears the water column of algae, why might this happen? (5) 8. List 5 values of ecosystem goods and services and how lake eutrophication might harm each one of them (10) 9. Fill in the following table by circling one of the two words in each cell of the right column (10) Property Reynolds number when water flows through fine sediments relative to coarse sediment Viscosity of water trying to filter bacteria as opposed to trying to filter larger algae Need to streamline for large swimming organisms compared to small ones (e.g. trout vs bacterium) Inertial of large swimming organisms compared to small ones (e.g. trout vs bacterium) Viscosity of cold water compared to warm water Surface area to volume ratio of small sphere compared to large sphere Rate of diffusion cause by molecular diffusion relative to turbulent flow (eddy diffusion) Light attenuation in a eutrophic versus an oligotrophic lake Attenuation of blue light relative to red light in an oligotrophic lake Density of ice compared to liquid water Relative amount or magnitude Larger smaller Greater Less More Less Greater Less Greater Less Greater Less Greater Less Greater Less Greater Less Greater Less 10. Why is it more difficult to reverse eutrophication once oxygen disappears from the hypolimmion as a lake becomes more eutrophic? (5) 11. List at least one author of the textbook for the class(5), if you get both then 2 points extra credit 12. What are the major goals of sewage treatment plants and how do they accomplish those goals? (10) 10