Product datasheet
Anti-Aspartate Aminotransferase antibody [EPR12145]
(HRP) ab201765
1 Image
Overview
Product name
Anti-Aspartate Aminotransferase antibody [EPR12145] (HRP)
Description
Rabbit monoclonal [EPR12145] to Aspartate Aminotransferase (HRP)
Conjugation
HRP
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Aspartate Aminotransferase aa 150-250 (Cysteine residue). The exact sequence is
proprietary.
Database link: P17174
Positive control
WB: HepG2; MCF-7 and K562 whole cell lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-Aspartate Aminotransferase antibody [EPR12145] (ab170950)
Anti-Aspartate Aminotransferase antibody (Alexa Fluor® 647) [EPR12145] (ab201043)
Anti-Aspartate Aminotransferase antibody (Alexa Fluor® 488) [EPR12145] (ab201269)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Affinity Purified
Clonality
Monoclonal
1
Clone number
EPR12145
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab201765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/5000. Detects a band of approximately 46 kDa (predicted molecular weight: 46
kDa).
Target
Function
Plays a key role in amino acid metabolism.
Sequence similarities
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Cellular localization
Cytoplasm.
Anti-Aspartate Aminotransferase antibody [EPR12145] (HRP) images
2
All lanes : Anti-Aspartate Aminotransferase
antibody [EPR12145] (HRP) (ab201765) at
1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver
carcinoma cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast
adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid
leukemia cell line) Whole Cell Lysate
Western blot - Anti-Aspartate Aminotransferase
Lysates/proteins at 10 µg per lane.
antibody [EPR12145] (HRP) (ab201765)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 46 kDa
Exposure time : 4 minutes
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 3% milk before being incubated with
ab201765 overnight at 4°C. Antibody binding
was visualised using ECL development
solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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