Product datasheet
Anti-MTCO2 antibody [EPR3314] (HRP) ab200615
2 Images
Overview
Product name
Anti-MTCO2 antibody [EPR3314] (HRP)
Description
Rabbit monoclonal [EPR3314] to MTCO2 (HRP)
Conjugation
HRP
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human MTCO2 aa 200 to the C-terminus.
Positive control
WB: K562, MCF7, THP1 and HeLa whole cell lysates. IHC-P: normal human liver tissue sections
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-MTCO2 antibody [EPR3314] (ab79393)
Anti-MTCO2 antibody (Alexa Fluor® 488) [EPR3314] (ab200613)
Anti-MTCO2 antibody (Alexa Fluor® 647) [EPR3314] (ab200525)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C. Store In the Dark.
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Purity
Affinity Purified
Clonality
Monoclonal
Clone number
EPR3314
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab200615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 25
kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
Target
Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of
oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2
transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic
center of the catalytic subunit 1.
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D)
[MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial
respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to
severe multisystem disease affecting several tissues and organs. Features include hypertrophic
cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise
intolerance, developmental delay, delayed motor development and mental retardation. A subset
of patients manifest Leigh syndrome.
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family.
Cellular localization
Mitochondrion inner membrane.
Anti-MTCO2 antibody [EPR3314] (HRP) images
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IHC image of MTCO2 staining in a section of
formalin-fixed paraffin-embedded normal
human liver*, performed on a Leica BOND.
The section was pre-treated using heat
mediated antigen retrieval with sodium citrate
buffer (pH6, epitope retrieval solution 1) for
20mins. The section was then incubated with
ab200615, 1/100 dilution, for 15 mins at room
temperature. DAB was used as the
chromogen. The section was then
counterstained with haematoxylin and
Immunohistochemistry (Formalin/PFA-fixed
mounted with DPX. The inset negative control
paraffin-embedded sections) - Anti-MTCO2
image is taken from an identical assay
antibody [EPR3314] (HRP) (ab200615)
without primary antibody.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
*Tissue obtained from the Human Research
Tissue Bank, supported by the NIHR
Cambridge Biomedical Research Centre
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All lanes : Anti-MTCO2 antibody [EPR3314]
(HRP) (ab200615) at 1/5000 dilution
Lane 1 : K562 (Human erythromyeloblastoid
leukemia cell line) Whole Cell Lysate
Lane 2 : MCF7 (Human breast
adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : THP1 (Human acute monocytic
leukemia cell line) Whole Cell Lysate
Lane 4 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Western blot - Anti-MTCO2 antibody [EPR3314]
(HRP) (ab200615)
Lysates/proteins at 10 µg per lane.
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 21 kDa
Exposure time : 30 seconds
This blot was produced using a 4-12% Bistris gel under the MES buffer system. The gel
was run at 200V for 35 minutes before being
transferred onto a Nitrocellulose membrane at
30V for 70 minutes. The membrane was then
blocked for an hour using 2% Bovine Serum
Albumin before being incubated with
ab200615 overnight at 4°C. Antibody binding
was visualised using ECL development
solution ab133406.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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