Product datasheet
Anti-Tyrosine Hydroxylase antibody [EP1533Y] ab75875
4 Abreviews 2 References 5 Images
Overview
Product name
Anti-Tyrosine Hydroxylase antibody [EP1533Y]
Description
Rabbit monoclonal [EP1533Y] to Tyrosine Hydroxylase
Tested applications
WB, ICC, Flow Cyt, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Tyrosine Hydroxylase aa 50-150 (phospho S70). The exact sequence is proprietary.
Database link: P07101
Positive control
Human adrenal gland lysate. This antibody gave a positive result in IF in the following
Formaldehyde fixed cell line: SKNSH. IHC-P: Human cerebral cortex FFPE tissue sections.
General notes
This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb®
technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or
7,429,487.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated ‘PUR’ on our product labels.
If you have any questions regarding this update, please contact our Scientific Support
team.
Alternative versions available:
Anti-Tyrosine Hydroxylase antibody (Alexa Fluor® 488) [EP1533Y] (ab192463)
Anti-Tyrosine Hydroxylase antibody (HRP) [EP1533Y] (ab193083)
Anti-Tyrosine Hydroxylase antibody (Phycoerythrin) [EP1533Y] (ab209921)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1533Y
1
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab75875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500. Predicted molecular weight: 59 kDa.
ICC
1/100 - 1/250.
Flow Cyt
1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control
with this antibody.
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.
Target
Function
Plays an important role in the physiology of adrenergic neurons.
Tissue specificity
Mainly expressed in the brain and adrenal glands.
Pathway
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Involvement in disease
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD)
[MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of
DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the
presence of sustained involuntary muscle contractions, often leading to abnormal postures.
Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of
dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy
number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient
but not in any controls.
Sequence similarities
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Anti-Tyrosine Hydroxylase antibody [EP1533Y] images
2
Anti-Tyrosine Hydroxylase antibody
[EP1533Y] (ab75875) at 1/500 dilution +
human adrenal gland lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/1000 dilution
Predicted band size : 59 kDa
Observed band size : 59 kDa
Western blot - Tyrosine Hydroxylase antibody
[EP1533Y] (ab75875)
ab75875 staining Tyrosine Hydroxylase in Pig
Myocardiun tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with formaldehyde
and blocked with 1% BSA for 10 minutes at
21°C; antigen retrieval was by heat mediation
in a citrate buffer. Samples were incubated
with primary antibody (1/1000 in blocking
Immunohistochemistry (Formalin/PFA-fixed
buffer) for 2 hours at 21°C. A Biotin-
paraffin-embedded sections) - Anti-Tyrosine
conjugated Goat anti-rabbit IgG polyclonal
Hydroxylase antibody [EP1533Y] (ab75875)
(1/250) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by
Carl Hobbs
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IHC image of Tyrosine Hydroxylase staining
in human cerebral cortex formalin fixed
paraffin embedded tissue section, performed
on a Leica Bond system using the standard
protocol F. The section was pre-treated using
heat mediated antigen retrieval with sodium
citrate buffer (pH6, epitope retrieval solution
1) for 20 mins. The section was then
incubated with ab75875, 1/200 dilution, for 15
mins at room temperature and detected using
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Tyrosine
Hydroxylase antibody [EP1533Y] (ab75875)
an HRP conjugated compact polymer system.
DAB was used as the chromogen. The
section was then counterstained with
haematoxylin and mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
ICC/IF image of ab75875 stained SKNSH
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab75875 at 1/100 dilution
overnight at +4°C. The secondary antibody
(green) was DyLight® 488 goat anti- rabbit
(ab96899) IgG (H+L) used at a 1/1000
Immunocytochemistry/ Immunofluorescence -
dilution for 1h. Alexa Fluor® 594 WGA was
Anti-Tyrosine Hydroxylase antibody [EP1533Y]
used to label plasma membranes (red) at a
(ab75875)
1/200 dilution for 1h. DAPI was used to stain
the cell nuclei (blue) at a concentration of
1.43µM.
4
Overlay histogram showing SH-SY5Y cells
stained with ab75875 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
protein-protein interactions followed by the
Flow Cytometry-Anti-Tyrosine Hydroxylase
antibody [EP1533Y](ab75875)
antibody (ab75875, 1/100 dilution) for 30 min
at 22ºC. The secondary antibody used was
DyLight® 488 goat anti-rabbit IgG (H+L)
(ab96899) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
rabbit IgG (monoclonal) (1µg/1x106 cells)
used under the same conditions. Acquisition
of >5,000 events was performed. This
antibody gave a positive signal in SH-SY5Y
cells fixed with 80% methanol (5
min)/permeabilized with 0.1% PBS-Tween for
20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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