Product datasheet
Anti-SMAD6 antibody ab63713
1 Abreviews 1 References 1 Image
Overview
Product name
Anti-SMAD6 antibody
Description
Rabbit polyclonal to SMAD6
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human
SMAD6.Read Abcam's proprietary immunogen policy(Peptide available as ab66351.)
Positive control
This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat,
A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab63713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa
(predicted molecular weight: 52 kDa).
Target
1
Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily
members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling
by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory
SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter
regions.
Tissue specificity
Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased
heart tissue.
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
Post-translational
modifications
Phosphorylated by BMP type 1 receptor kinase and by PRKX.
Ubiquitinated by WWP1.
Cellular localization
Nucleus.
Anti-SMAD6 antibody images
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All lanes : Anti-SMAD6 antibody (ab63713)
at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblastlike cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate
Western blot - SMAD6 antibody (ab63713)
Lane 4 : HEK293 (Human embryonic kidney
cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver
carcinoma cell line) Whole Cell Lysate
Lane 6 : MCF7 (Human breast
adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma
cell line) Whole Cell Lysate
Lane 8 : U2OS (Human osteosarcoma cell
line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - PreAdsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 130 kDa,70 kDa. We
are unsure as to the identity of these extra
bands.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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