Brian M. Paegel
Associate Professor
Department of Chemistry
The Scripps Research Institute
Jupiter, FL 33458 USA
+1.561.228.2754 (O)
+1.561.228.3092 (F)
+1.619.840.0811 (M)
briandna@scripps.edu
EDUCATION
Ph. D., Chemistry, University of California, Berkeley, CA (2003)
B. S., Chemistry, magna cum laude, Duke University, Durham, NC (1998)
APPOINTMENTS
2014 –
Associate Professor, Department of Chemistry
The Scripps Research Institute, Jupiter, FL
2008 – 2014
Assistant Professor, Department of Chemistry,
The Scripps Research Institute, Jupiter, FL
2004 – 2008
NIH NRSA Postdoctoral Fellow, Department of Molecular Biology
The Scripps Research Institute, La Jolla, CA (Gerald F. Joyce, advisor)
1998 – 2003
Doctoral Student, Department of Chemistry
University of California, Berkeley, CA (Richard A. Mathies, advisor)
AWARDS
NSF CAREER Award (2013)
NIH Director’s New Innovator Award (2011)
NIH Pathway to Independence Award (2007)
NIH NRSA Postdoctoral Fellowship (2005)
NIH Program in Genomics Fellow (2000)
ACS Division of Analytical Chemistry Fellow (2000)
Hypercube Scholar (1998)
ACTIVITIES
2012 –
2012 –
2012 – 2013
2011 –
2011
2011
2010 – 2014
2009 –
2009 –
2008
2007 – 2013
Systems and Synthetic Biology Panel, NSF, Washington, D.C.
Academic Advisory Committee, TSRI, Jupiter, FL
Florida Theme Committee, TSRI, Jupiter, FL
SAB, GigaGen, San Francisco, CA
Exobiology Panel, NASA, Washington, D.C.
Executive Technical Planning Committee, MicroTAS, Seattle, WA
Chair, External Seminar Series Committee, TSRI, Jupiter, FL
Faculty Advisor, Education Outreach, TSRI, Jupiter, FL
Chair, Graduate Admissions Committee, TSRI, Jupiter, FL
Local Planning Committee, MicroTAS, San Diego, CA
Co-founder and SAB, Allopartis Biotechnologies, San Francisco, CA
PUBLICATIONS
A. K. Price, B. M. Paegel. “Discovery in Droplets.” Anal. Chem. 2016 (in press).
A. K. Price, A. B. MacConnell, B. M. Paegel. “hvSABR: Photochemical Dose-Response Bead
Screening in Droplets.” 2015 (submitted).
M. L. Malone, B. M. Paegel. “What is a ‘DNA-Compatible’ Reaction?” 2015 (submitted).
A. B. MacConnell, P. J. McEnaney, V. J. Cavett, B. M. Paegel. “DNA-Encoded Solid-Phase Synthesis:
Encoding Language Design and Complex Oligomer Library Synthesis.” ACS Combi. Sci. 2015, 17,
518.
A. K. Price, A. B. MacConnell, B. M. Paegel. “Microfluidic Bead Suspension Hopper.” Anal. Chem.
2014, 86, 5039.
S. Matosevic, B. M. Paegel. “Layer-by-Layer Cell Membrane Assembly.” Nat. Chem., 2013, 5, 958.
S. Matosevic, B. M. Paegel. “Stepwise Synthesis of Giant Unilamellar Vesicles on a Microfluidic
Assembly Line.” J. Am. Chem. Soc., 2011, 133, 2798.
B. M. Paegel. “Microfluidic Landscapes for Evolution.” Curr. Opin. Chem. Biol., 2010, 14, 568.
B.M. Paegel, G. F. Joyce. “Microfluidic Compartmentalized Directed Evolution.” Chem. Biol., 2010,
17, 717.
B.M. Paegel, G. F. Joyce. “Darwinian Evolution on a Chip.” PLoS Biol., 2008, 6, 900.
B. M. Paegel, W. H. Grover, A. M. Skelley, R. A. Mathies, G. F. Joyce. “Microfluidic Serial Dilution
Circuit.” Anal. Chem., 2006, 78, 7522.
E. A. S. Doherty, C. Kan, B. M. Paegel, S. H. I. Yeung, S. Cao, R. A. Mathies, A. E. Barron “Sparsely
Cross-Linked ‘Nanogel’ Matrixes as Fluid, Mechanically Stabilized Polymer Networks for HighThroughput Microchannel DNA Sequencing.” Anal. Chem., 2004, 76, 5249.
T. Kamei, B. M. Paegel, J. R. Scherer, A. M. Skelley, R. A. Street, R. A. Mathies. “Integrated
Hydrogenated Amorphous Si Photodiode Detector for Microfluidic Bioanalytical Devices.” Anal.
Chem., 2003, 75, 5300.
B. M. Paegel, R. G. Blazej, R. A. Mathies. “Microfluidic Devices for DNA Sequencing Sample
Preparation and Electrophoretic Analysis.” Curr. Opin. Biotechnol., 2003, 14, 42.
R. G. Blazej, B. M. Paegel, R. A. Mathies. “SNP Discovery and Screening by Polymorphism Ratio
Sequencing.” Genome Res., 2003, 13, 287.
B. M. Paegel, S. H. I. Yeung, R. A. Mathies. “Microchip Bioprocessor for Integrated Nanovolume
Sample Purification and DNA Sequencing.” Anal. Chem., 2002, 74, 5092.
B. M. Paegel, C. A. Emrich, G. J. Wedemayer, J. R. Scherer, R. A. Mathies. “High-Throughput DNA
Sequencing with a Microfabricated 96-Lane Capillary Array Electrophoresis Bioprocessor.” Proc.
Natl. Acad. Sci. U. S. A., 2002, 99, 574.
J. R. Scherer, B. M. Paegel, G. J. Wedemayer, C. A. Emrich, J. Lo, I. L. Medintz, R. A. Mathies. “HighPressure Gel Loader for Capillary Array Electrophoresis Microchannel Plates.” Biotechniques, 2001,
31, 1150.
I. L. Medintz, B. M. Paegel, R. G. Blazej, C. A. Emrich, L. Berti, J. R. Scherer, R. A. Mathies. “HighPerformance Genetic Analysis Using Microfabricated Capillary Array Electrophoresis.”
Electrophoresis, 2001, 22, 3845.
I. L. Medintz, B. M. Paegel, R. A. Mathies. “Microfabricated Capillary Array Electrophoresis DNA
Analysis Systems.” J. Chromatogr. A, 2001, 924, 265.
B. M. Paegel, L. D. Hutt, P. C. Simpson, R. A. Mathies. “Turn Geometry for Minimizing Band
Broadening in Microfabricated Capillary Electrophoresis Channels.” Anal. Chem., 2000, 72, 3030.
BOOK CHAPTERS
B. M. Paegel, R. G. Blazej, R. A. Mathies, “Microfabricated Devices for DNA Sequencing” in
Analytical Techniques in DNA Sequencing. Brian K. Nunally, Ed. Marcel Drekker, New York, 2003.
R. G. Blazej, B. M. Paegel, C. A. Emrich, R. A. Mathies, “Microfabricated Capillary Array
Electrophoresis: Implementation and Applications” in Lab-on-a-chip: Chemistry in Miniaturized
Synthesis and Analysis Systems. Albert van den Berg, Ed, Elsevier, Amsterdam, The Netherlands,
2003.
PATENTS
Brian M. Paegel, Andrew B. MacConnell, Patrick J. McEnaney, Thomas Kodadek, “DNA-based beadspecific barcoding for DNA-encoded solid phase library synthesis.”
Brian M. Paegel & Robert G. Blazej, “Method of Enhancing Enzyme Activity.”
Brian M. Paegel, William H. Grover, Alison M. Skelley, Richard A. Mathies, Gerald F. Joyce,
“Microfluidic Serial Dilution Circuit.”
William G. Grover, Alison M. Skelley, Chung N. Liu, Eric T. Lagally, Brian M. Paegel, Richard A.
Mathies, “Methods and Apparatus for Pathogen Detection and Analysis.”
Brian M. Paegel, Lester D. Hutt, Peter C. Simpson, Richard A. Mathies, “Apparatus and Methods for
High Resolution Separation of Sample Components on Microfabricated Channel Devices.”
Brian M. Paegel & Gerald F. Joyce, “Biochemical Applications of a Microfluidic Serial Dilution
Circuit.”
Brian M. Paegel, William H. Grover, Alison M. Skelley, Richard A. Mathies, Gerald F. Joyce,
“Microfluidic Serial Dilution Circuit.”
PRESENTATIONS
“Cells Under Construction.” FNANO, Snowbird, UT (2016)
“The Revolution will be Compartmentalized.” Duke University, Durham, NC (2016)
“The Revolution will be Compartmentalized.” University of Florida, Gainesville, FL (2016)
“The Revolution will be Compartmentalized.” NIST, Gaithersburg, MD (2015)
“The Revolution will be Compartmentalized.” The Ohio State University, Columbus, NC (2015)
“The Revolution will be Compartmentalized.” Caltech, Pasadena, CA (2014)
“The Revolution will be Compartmentalized.” Florida Atlantic University, Boca Raton, FL (2014)
“The Revolution will be Compartmentalized.” Florida Southern College, Lakeland, FL (2014)
“The Revolution will be Compartmentalized.” University of Texas, Austin, TX (2014)
“The Revolution will be Compartmentalized.” The Scripps Research Institute, Jupiter, FL (2013)
“The Revolution will be Compartmentalized.” Furman University, Greenville, SC (2013)
“Distributed Drug Discovery.” Florida State University, Tallahassee, FL (2013)
“Distributed Drug Discovery.” Pennsylvania State University, State College, PA (2013)
“Distributed Drug Discovery.” Society for Lab Automation and Screening, Orlando, FL (2013)
“Cells Under Construction.” Florida Atlantic University, Boca Raton, FL (2012)
“Total Synthesis of a Cell.” UC Berkeley, Berkeley, CA, (2011)
“Cells Under Construction.” NIST, Gaithersburg, MD (2011)
“Cells Under Construction.” University of Maryland, Shady Grove, MD (2011)
“Cells Under Construction.” The Scripps Research Institute, Jupiter, FL (2011)
“New Protease Tools for Phospho-proteomics.” Cell Signaling Technology, Beverly, MA (2010)
“Microfluidic Compartmentalized Directed Evolution.” µTAS, Groningen, The Netherlands, (2010)
“Oceans and Archipelagos: Landscapes for Extracellular Darwinian Evolution.” Furman University,
Greenville, SC (2010)
“Oceans and Archipelagos: Landscapes for Extracellular Darwinian Evolution.” Second Annual
Lasker Lectures, Jupiter, FL (2010)
“Microfluidic Landscapes for Extracellular Darwinian Evolution.” ASME 2010 First Global Congress
on NanoEngineering for Medicine and Biology, Houston, TX (2010).
“Oceans and Archipelagos: Landscapes for Extracellular Darwinian Evolution.” NIST, Gaithersburg,
MD (2009)
“Microfluidic Darwinian Evolution.” µTAS, San Diego, CA (2008)
“Microfluidic Darwinian Evolution.” The Scripps Research Institute, Jupiter, FL (2008)
“Microfluidic Darwinian Evolution.” LS9, Inc., San Francisco, CA (2008)
“Microfluidic Darwinian Evolution.” UCSF, San Francisco, CA (2008)
“Microfluidic Darwinian Evolution.” Caltech, Pasadena, CA (2008)
“Microfluidic Evolution of RNA Catalysts.” TSRI Society of Fellows Fall Research Symposium, La
Jolla, CA (2006)
“Microfluidic Serial Transfer Circuit: Automated Evolution of RNA Catalysts.” NIST, Gaithersburg,
MD (2006)
“Microfluidic Evolution of RNA Catalysts.” µTAS, Boston, MA, (2005)
“Microfluidic Circuit for Integrated Sample Purification and Electrophoretic Analysis: Application to
DNA Sequencing.” PittCon, Orlando, FL (2003).
“Integrated Bioprocessors for Nano-volume DNA Sequencing Sample Processing and Analysis.”
μTAS, Nara, Japan (2002)
“Microfabricated Capillary Array Electrophoresis: High-Throughput DNA Sequencing and
Polymorphism Analysis.” μTAS, Monterrey, CA (2001)
“Minimization of Turn-Induced Band Broadening in Microfabricated Capillary Electrophoresis
Devices.” Frederick Conference on Capillary Electrophoresis, Frederick, MD (1999)
TRAINEES
Andrew MacConnell
Marie Malone
Wesley Cochrane
TSRI Graduate Student
TSRI Graduate Student
TSRI Graduate Student
2011 –
2013 –
2014 –
Sandro Matosevic
Josh Kostera
Alex Price
Duc Tran
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
Postdoctoral Fellow
2009 – 2013
2010 – 2013
2010 –
2013 –
Lillian McBee
Andrew Acevedo
Christine Chapman
Tate Storey
Vuong Dang
Gage Brummer
Max Morgenstern
Michael Richmond
Byron Weiss
Reuben Moncada
Karla Montejo
Stanford (UG)
Washington U. St. Louis (UG)
Brown (UG)
U. Florida (UG)
Swarthmore (UG)
Kansas State U. (UG)
U. Penn. (UG)
Florida Atlantic U. (UG)
Columbia (UG)
Vassar (UG)
Florida International University (UG)
2009 – 2011; 2013
2009 – 2010; 2013
2009 – 2011
2010 – 2012
2010
2011
2011 – 2012
2011 – 2012
2013
2014
2015
Joseph Pellizi
John Cassel
Suyash Kumar
Claire Dykas
Brett Gileau
John Lenard High School
Wellington High School
Boca Raton High School
Spanish River High School
Wellington High School
2009
2010
2011
2012
2013
FUNDING
2014 – 2018
DARPA Fold F(x) Program
DNA-Encoded Molecular Library Synthesis and HTS Platform Engineering
2013 – 2018
NSF CAREER Award (MCB1255250)
Total Synthesis of a Cell
2011 – 2016
NIH Director’s New Innovator Award (DP2OD008535)
Evolving and Engineering New Protease Tools for Mass Spectrometry Proteomics
2007 – 2011
NIH Pathway to Independence Award (R00GM083155)
Microfluidic Processors for Directed Evolution and Synthetic Biology
BIO
Brian M. Paegel is associate professor in the Department of Chemistry at The Scripps Research
Institute. He is interested in the assembly of cell-like compartments and the unique chemistry and
biology that can be conducted in their confines. He studies cellular membrane assembly, evolution
of new proteases for mass spectrometry-based proteomics, DNA-encoded compound library
synthesis, and picoliter-scale compound screening.
Paegel earned his undergraduate degree in chemistry from Duke University and his doctoral degree
in chemistry from UC Berkeley as a student of Richard Mathies. He pursued postdoctoral studies in
chemical biology and molecular evolution under the mentorship of Gerald Joyce at The Scripps
Research Institute, where he was the recipient of both a NIH National Research Service Award and a
Pathway to Independence Award. In 2008 he was appointed to the chemistry faculty and relocated
to TSRI’s new east coast campus in South Florida where he received a NIH Director’s New Innovator
award and a NSF CAREER award.