Product datasheet
Anti-Myosin light chain antibody [MY-21] ab11082
2 Abreviews 4 References 1 Image
Overview
Product name
Anti-Myosin light chain antibody [MY-21]
Description
Mouse monoclonal [MY-21] to Myosin light chain
Tested applications
ICC/IF, Dot Blot, WB
Species reactivity
Reacts with: Rabbit, Chicken, Cow, Human, Pig
Immunogen
Chicken lens membranes.
Positive control
WB: chicken gizzard, intestine or pig stomach lysate ICC/IF: stress fibers of well spread chicken
or human fibroblast cells
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged
storage, clarify by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Purity
Ascites
Clonality
Monoclonal
Clone number
MY-21
Isotype
IgM
Applications
Our Abpromise guarantee covers the use of ab11082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/200.
Dot Blot
Use at an assay dependent dilution. Use at an assay dependent dilution. The
antibody reacts with purified myosin light chains (20,000 daltons) in both the
native protein state or an SDS-heat denatured, reduced state.
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Application
Abreviews
Notes
WB
1/5000. Detects a band of approximately 20 kDa. 1/5000 (see review). Detects a
band of approximately 20 kDa. The product shows no reactivity with intact myosin
molecules in their native state. Heat denaturation in the presence of SDS and a
reducing agent (e.g. mercaptoethanol) makes accessible for the monoclonal
antibody an epitope on the myosin light chains.
Target
Function
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and
nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor
capping, and cell locomotion.
Tissue specificity
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Sequence similarities
Contains 3 EF-hand domains.
Post-translational
modifications
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the
contractile activity. It is required to generate the driving force in the migration of the cells but not
necessary for localization of myosin-2 at the leading edge.
Anti-Myosin light chain antibody [MY-21] images
ab11082, at /200 dilution, staining 20kDa
myosin light chains in PTK2 cells by
immunoflourescence.
Immunocytochemistry/ Immunofluorescence Myosin light chain antibody [MY-21] (ab11082)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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