Product datasheet
Anti-Slingshot homolog 1 antibody ab76943
5 References 3 Images
Overview
Product name
Anti-Slingshot homolog 1 antibody
Description
Rabbit polyclonal to Slingshot homolog 1
Tested applications
WB, IP, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Gorilla, Orangutan
Immunogen
Synthetic peptide corresponding to a region between residues 675 and 725 of human Slingshot
homolog 1 (NP_061857.2)
Positive control
HeLa and 293T whole cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Purity
Immunogen affinity purified
Purification notes
ab76943 was affinity purified using an epitope specific to Slingshot homolog 1 immobilized on
solid support.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab76943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/2000 - 1/10000. Detects a band of approximately 150 kDa (predicted
molecular weight: 116 kDa).
IP
Use at 2-5 - 5 µg/mg of lysate.
ICC/IF
Use a concentration of 1 µg/ml.
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Target
Function
Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates
the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and
stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which
may also be dephosphorylated and inactivated by this protein.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Post-translational
modifications
Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ.
Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and
lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A.
Phosphorylation decreases immediately prior to telophase.
Cellular localization
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cleavage furrow. Midbody. Also
recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control
of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the
midbody during cytokinesis.
Anti-Slingshot homolog 1 antibody images
All lanes : Anti-Slingshot homolog 1 antibody
(ab76943) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Western blot - Slingshot homolog 1 antibody
(ab76943)
Predicted band size : 116 kDa
Observed band size : 150 kDa
ab76943 at 1µg/ml staining Slingshot
homolog 1 in HeLa whole cell lysate
immunoprecipitated using ab76943 at
3µg/mg lysate (1 mg/IP; 20% of IP
loaded/lane).
Immunoprecipitation - Slingshot homolog 1
antibody (ab76943)
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ICC/IF image of ab76943 stained HeLa cells.
The cells were 4% formaldehyde fixed (10
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab76943, 1µg/ml) overnight
at +4°C. The secondary antibody (green) was
Alexa Fluor® 488 goat anti-rabbit IgG (H+L)
used at a 1/1000 dilution for 1h. Alexa Fluor®
Immunocytochemistry/ Immunofluorescence-
594 WGA was used to label plasma
Slingshot homolog 1 antibody(ab76943)
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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