Product datasheet
Anti-SKIP antibody ab113441
2 Images
Overview
Product name
Anti-SKIP antibody
Description
Rabbit polyclonal to SKIP
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen
Recombinant fragment, corresponding to amino acids 183-430 of Human SKIP (BC004362).
Positive control
Human fetal heart lysate; Human fetal skeletal muscle tissue.
Properties
Form
Lyophilised:Reconstitute in 200ul sterile H2O
Storage instructions
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Purity
Immunogen affinity purified
Purification notes
ab113441 is purified by a protein affinity column.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab113441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular
weight: 51 kDa).
IHC-P
1/100 - 1/500.
Target
1
Function
Inositol 5-phosphatase which acts on inositol 1,4,5-trisphosphate, inositol 1,3,4,5tetrakisphosphate, phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5triphosphate. Has 6-fold higher affinity for phosphatidylinositol 4,5-bisphosphate than for inositol
1,4,5-trisphosphate. May negatively regulate assembly of the actin cytoskeleton.
Tissue specificity
Ubiquitously expressed with highest levels in skeletal muscle, heart and kidney.
Sequence similarities
Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
Cellular localization
Endoplasmic reticulum. Following stimulation with EGF, translocates to membrane ruffles.
Anti-SKIP antibody images
Anti-SKIP antibody (ab113441) at 1/500
dilution + Human fetal heart lysate
Predicted band size : 51 kDa
Western blot - SKIP antibody (ab113441)
ab113441 at 1/100 dilution staining SKIP in
Formalin-fixed, Paraffin-embedded Human
fetal skeletal muscle tissue by
Immunohistochemistry. Note cytoplasmic
staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - SKIP antibody
(ab113441)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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