Product datasheet
Anti-MYO9B antibody ab52026
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Overview
Product name
Anti-MYO9B antibody
Description
Goat polyclonal to MYO9B
Tested applications
ELISA, WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide:
C-KNDFEPVKQSKAQ
, corresponding to internal sequence amino acids 1605-1617 of Human MYO9B
Run BLAST with
Positive control
Run BLAST with
Human Liver lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab52026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ELISA
WB
Application notes
Peptide ELISA: Antibody detection limit dilution 1:2,000.
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WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted
molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Myosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I
and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte
differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and
Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B).
The isoforms of myosin IX have similar overall domain structure and are expressed in many
tissues and cell types, although they have distinct tissue expression patterns. The subcellular
localization of class IX myosins appears to be partly cytoplasmic, and partly associated with
membranes and the actin cytoskeleton. The expression level of myosins IX may vary during
development and differentiation.
Cellular localization
Cytoplasm; cell cortex. Cytoplasm; perinuclear region. Note=In undifferentiated cells colocalizes
with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the
highest levels in the perinuclear region.
Anti-MYO9B antibody images
Anti-MYO9B antibody (ab52026) at 2 µg/ml +
Human Liver lysate (35µg protein in RIPA
buffer).
Predicted band size : 244 kDa
Observed band size : 200 kDa
Western blot - MYO9B antibody (ab52026)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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